Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053932.t1 | XP_013452693.1 | 98.2 | 399 | 7 | 0 | 1 | 399 | 1 | 399 | 2.70E-231 | 810.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053932.t1 | A0A072UB96 | 98.2 | 399 | 7 | 0 | 1 | 399 | 1 | 399 | 2.0e-231 | 810.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene053932 | 0.827016 | 1.97E-54 | -1.69E-46 |
| MS.gene049457 | MS.gene053932 | 0.802507 | 5.43E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene053932 | 0.801382 | 9.24E-49 | -1.69E-46 |
| MS.gene051959 | MS.gene053932 | 0.861396 | 1.06E-63 | -1.69E-46 |
| MS.gene051960 | MS.gene053932 | 0.857533 | 1.53E-62 | -1.69E-46 |
| MS.gene052053 | MS.gene053932 | 0.82584 | 3.76E-54 | -1.69E-46 |
| MS.gene052091 | MS.gene053932 | 0.847203 | 1.34E-59 | -1.69E-46 |
| MS.gene053932 | MS.gene055549 | 0.839115 | 1.92E-57 | -1.69E-46 |
| MS.gene053932 | MS.gene055550 | 0.844245 | 8.52E-59 | -1.69E-46 |
| MS.gene053932 | MS.gene055551 | 0.831807 | 1.35E-55 | -1.69E-46 |
| MS.gene053932 | MS.gene056365 | 0.817903 | 2.59E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene057001 | 0.852194 | 5.42E-61 | -1.69E-46 |
| MS.gene053932 | MS.gene06012 | 0.80632 | 8.71E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene060237 | 0.826875 | 2.13E-54 | -1.69E-46 |
| MS.gene053932 | MS.gene06023 | 0.815441 | 9.23E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene06130 | 0.817146 | 3.84E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene063094 | 0.822608 | 2.16E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene064275 | 0.888003 | 8.68E-73 | -1.69E-46 |
| MS.gene053932 | MS.gene065073 | 0.821439 | 4.04E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene065089 | 0.807653 | 4.55E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene065509 | 0.811492 | 6.81E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene067477 | 0.844604 | 6.82E-59 | -1.69E-46 |
| MS.gene053932 | MS.gene070320 | 0.836786 | 7.63E-57 | -1.69E-46 |
| MS.gene053932 | MS.gene074124 | 0.801958 | 7.04E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene08120 | 0.818514 | 1.88E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene09926 | 0.815123 | 1.09E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene09927 | 0.819857 | 9.32E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene24099 | 0.860311 | 2.26E-63 | -1.69E-46 |
| MS.gene053932 | MS.gene25530 | 0.809082 | 2.26E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene27099 | 0.869401 | 3.18E-66 | -1.69E-46 |
| MS.gene053932 | MS.gene27204 | 0.806366 | 8.52E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene28352 | 0.808779 | 2.62E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene28828 | 0.810584 | 1.07E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene31091 | 0.827969 | 1.17E-54 | -1.69E-46 |
| MS.gene053932 | MS.gene31130 | 0.851201 | 1.04E-60 | -1.69E-46 |
| MS.gene053932 | MS.gene31861 | 0.830931 | 2.22E-55 | -1.69E-46 |
| MS.gene053932 | MS.gene31868 | 0.813629 | 2.32E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene33645 | 0.825454 | 4.65E-54 | -1.69E-46 |
| MS.gene053932 | MS.gene34636 | 0.811244 | 7.71E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene36266 | 0.804987 | 1.66E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene36267 | 0.818834 | 1.59E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene36464 | 0.81987 | 9.25E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene36749 | 0.812466 | 4.18E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene36830 | 0.823896 | 1.08E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene38536 | 0.81306 | 3.10E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene39380 | 0.802528 | 5.38E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene39743 | 0.80016 | 1.64E-48 | -1.69E-46 |
| MS.gene053932 | MS.gene40619 | 0.842336 | 2.75E-58 | -1.69E-46 |
| MS.gene053932 | MS.gene40620 | 0.848252 | 6.90E-60 | -1.69E-46 |
| MS.gene053932 | MS.gene42130 | 0.859501 | 3.96E-63 | -1.69E-46 |
| MS.gene053932 | MS.gene46919 | 0.821333 | 4.27E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene51514 | 0.815715 | 8.02E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene54570 | 0.809622 | 1.73E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene54571 | 0.811494 | 6.81E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene55745 | 0.801482 | 8.82E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene55755 | 0.816713 | 4.80E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene56667 | 0.813007 | 3.18E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene57332 | 0.815863 | 7.43E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene57465 | 0.803044 | 4.21E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene58691 | 0.808738 | 2.67E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene60161 | 0.81522 | 1.03E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene61746 | 0.818073 | 2.37E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene67236 | 0.850199 | 1.98E-60 | -1.69E-46 |
| MS.gene053932 | MS.gene67744 | 0.827672 | 1.37E-54 | -1.69E-46 |
| MS.gene053932 | MS.gene68141 | 0.819526 | 1.11E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene69210 | 0.88626 | 3.99E-72 | -1.69E-46 |
| MS.gene053932 | MS.gene72157 | 0.830886 | 2.28E-55 | -1.69E-46 |
| MS.gene053932 | MS.gene72160 | 0.819151 | 1.35E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene72178 | 0.820787 | 5.70E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene72198 | 0.833389 | 5.49E-56 | -1.69E-46 |
| MS.gene053932 | MS.gene73393 | 0.810019 | 1.42E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene74337 | 0.815953 | 7.10E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene76159 | 0.855991 | 4.35E-62 | -1.69E-46 |
| MS.gene053932 | MS.gene76423 | 0.832285 | 1.03E-55 | -1.69E-46 |
| MS.gene053932 | MS.gene77183 | 0.810598 | 1.06E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene78383 | 0.836827 | 7.45E-57 | -1.69E-46 |
| MS.gene053932 | MS.gene78419 | 0.81221 | 4.75E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene78745 | 0.882518 | 9.74E-71 | -1.69E-46 |
| MS.gene053932 | MS.gene81108 | 0.810899 | 9.16E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene82549 | 0.80785 | 4.13E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene83604 | 0.826229 | 3.04E-54 | -1.69E-46 |
| MS.gene053932 | MS.gene85232 | 0.849893 | 2.41E-60 | -1.69E-46 |
| MS.gene053932 | MS.gene87017 | 0.83744 | 5.19E-57 | -1.69E-46 |
| MS.gene053932 | MS.gene87798 | 0.827665 | 1.38E-54 | -1.69E-46 |
| MS.gene053932 | MS.gene88946 | 0.839318 | 1.70E-57 | -1.69E-46 |
| MS.gene053932 | MS.gene88977 | 0.851818 | 6.93E-61 | -1.69E-46 |
| MS.gene053932 | MS.gene89083 | 0.803897 | 2.80E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene89256 | 0.8025 | 5.45E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene89702 | 0.833428 | 5.37E-56 | -1.69E-46 |
| MS.gene053932 | MS.gene90362 | 0.83515 | 1.98E-56 | -1.69E-46 |
| MS.gene053932 | MS.gene93160 | 0.862606 | 4.50E-64 | -1.69E-46 |
| MS.gene053932 | MS.gene93163 | 0.817611 | 3.01E-52 | -1.69E-46 |
| MS.gene053932 | MS.gene95714 | 0.829577 | 4.76E-55 | -1.69E-46 |
| MS.gene053932 | MS.gene95833 | 0.83024 | 3.28E-55 | -1.69E-46 |
| MS.gene053932 | MS.gene99711 | 0.821471 | 3.97E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027537 | MS.gene053932 | PPI |
| MS.gene45116 | MS.gene053932 | PPI |
| MS.gene68298 | MS.gene053932 | PPI |
| MS.gene053932 | MS.gene042700 | PPI |
| MS.gene045965 | MS.gene053932 | PPI |
| MS.gene027539 | MS.gene053932 | PPI |
| MS.gene68293 | MS.gene053932 | PPI |
| MS.gene027535 | MS.gene053932 | PPI |
| MS.gene68297 | MS.gene053932 | PPI |
| MS.gene027538 | MS.gene053932 | PPI |
| MS.gene45115 | MS.gene053932 | PPI |
| MS.gene027540 | MS.gene053932 | PPI |
| MS.gene43936 | MS.gene053932 | PPI |
| MS.gene45118 | MS.gene053932 | PPI |
| MS.gene68296 | MS.gene053932 | PPI |
| MS.gene027536 | MS.gene053932 | PPI |
| MS.gene68292 | MS.gene053932 | PPI |
| MS.gene027543 | MS.gene053932 | PPI |
| MS.gene45117 | MS.gene053932 | PPI |
| MS.gene68295 | MS.gene053932 | PPI |
| MS.gene053932 | MS.gene78745 | PPI |
| MS.gene43935 | MS.gene053932 | PPI |
| MS.gene45114 | MS.gene053932 | PPI |
| MS.gene053932 | MS.gene027536 | PPI |
| MS.gene027544 | MS.gene053932 | PPI |
| MS.gene027534 | MS.gene053932 | PPI |
| MS.gene45119 | MS.gene053932 | PPI |
| MS.gene45120 | MS.gene053932 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053932.t1 | MTR_6g472110 | 98.246 | 399 | 7 | 0 | 1 | 399 | 1 | 399 | 0.0 | 817 |
| MS.gene053932.t1 | MTR_5g035690 | 50.985 | 406 | 172 | 12 | 1 | 399 | 1 | 386 | 8.47e-131 | 381 |
| MS.gene053932.t1 | MTR_5g035665 | 51.000 | 400 | 174 | 9 | 1 | 399 | 1 | 379 | 2.25e-128 | 375 |
| MS.gene053932.t1 | MTR_5g035750 | 49.505 | 404 | 182 | 10 | 1 | 399 | 1 | 387 | 2.74e-125 | 367 |
| MS.gene053932.t1 | MTR_5g035760 | 50.125 | 401 | 177 | 11 | 1 | 399 | 1 | 380 | 1.30e-121 | 357 |
| MS.gene053932.t1 | MTR_5g035670 | 48.750 | 400 | 185 | 8 | 1 | 399 | 1 | 381 | 2.10e-120 | 354 |
| MS.gene053932.t1 | MTR_6g016660 | 42.298 | 409 | 215 | 10 | 1 | 399 | 1 | 398 | 8.20e-102 | 307 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053932.t1 | AT1G13480 | 43.781 | 402 | 208 | 9 | 1 | 399 | 1 | 387 | 4.83e-104 | 313 |
| MS.gene053932.t1 | AT1G13520 | 44.307 | 404 | 203 | 10 | 1 | 399 | 1 | 387 | 9.91e-102 | 307 |
| MS.gene053932.t1 | AT1G13530 | 44.802 | 404 | 199 | 10 | 1 | 399 | 1 | 385 | 3.71e-97 | 295 |
| MS.gene053932.t1 | AT1G13490 | 43.424 | 403 | 203 | 8 | 1 | 399 | 1 | 382 | 3.93e-93 | 285 |
| MS.gene053932.t1 | AT1G13500 | 45.343 | 408 | 192 | 12 | 1 | 399 | 1 | 386 | 1.76e-92 | 283 |
| MS.gene053932.t1 | AT1G13470 | 44.665 | 403 | 194 | 14 | 1 | 399 | 1 | 378 | 1.82e-90 | 278 |
| MS.gene053932.t1 | AT1G13490 | 42.537 | 402 | 189 | 9 | 1 | 399 | 1 | 363 | 2.15e-87 | 270 |
| MS.gene053932.t1 | AT1G13540 | 41.176 | 408 | 204 | 14 | 1 | 399 | 1 | 381 | 2.41e-87 | 270 |
| MS.gene053932.t1 | AT1G61600 | 38.837 | 430 | 223 | 11 | 1 | 399 | 1 | 421 | 1.40e-84 | 264 |
| MS.gene053932.t1 | AT1G13510 | 42.118 | 406 | 202 | 14 | 1 | 399 | 1 | 380 | 1.72e-84 | 263 |
| MS.gene053932.t1 | AT1G13550 | 39.851 | 404 | 216 | 9 | 1 | 396 | 1 | 385 | 1.15e-79 | 250 |
Find 88 sgRNAs with CRISPR-Local
Find 139 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACCAAAACACCAGAATT+AGG | 0.174233 | 5.3:+42490712 | None:intergenic |
| AGAATTTGAAGGGACTAATA+AGG | 0.248217 | 5.3:-42489073 | MS.gene053932:CDS |
| TGATAATGATGATGGTAAAA+AGG | 0.249854 | 5.3:-42489340 | MS.gene053932:CDS |
| TTGAAAACAACCTCTTGTTT+TGG | 0.266662 | 5.3:-42490676 | MS.gene053932:CDS |
| AGGCCCTAATTCTGGTGTTT+TGG | 0.301149 | 5.3:-42490716 | MS.gene053932:CDS |
| GCTATGGTGAAATCGAATTC+TGG | 0.330908 | 5.3:+42489516 | None:intergenic |
| CCACCAGAAGGCCCTAATTC+TGG | 0.339823 | 5.3:-42490724 | MS.gene053932:CDS |
| CTGATATCTAAAGGATGAAT+TGG | 0.361943 | 5.3:+42489738 | None:intergenic |
| ACACCAGAATTAGGGCCTTC+TGG | 0.363127 | 5.3:+42490721 | None:intergenic |
| AGATTACACTAGAGCAGAAA+TGG | 0.366742 | 5.3:-42489380 | MS.gene053932:CDS |
| CAGGAGAGGGTTGGATGGCT+TGG | 0.376011 | 5.3:-42489132 | MS.gene053932:CDS |
| GGGAGAGTACCATGAAGTTA+AGG | 0.382423 | 5.3:-42490650 | MS.gene053932:CDS |
| TACTATGTCATACATCTTCA+TGG | 0.383281 | 5.3:-42490499 | MS.gene053932:CDS |
| GAACAGTGTGGGTTTGAGTA+TGG | 0.387431 | 5.3:-42489181 | MS.gene053932:CDS |
| CCCTTCATGTTTATCAAAGA+AGG | 0.391686 | 5.3:-42489450 | MS.gene053932:CDS |
| AAAGTGTTGTTGTGGGAAAA+TGG | 0.398385 | 5.3:-42489479 | MS.gene053932:CDS |
| ACATCAAGTGATTCAAGTCT+TGG | 0.402196 | 5.3:-42489579 | MS.gene053932:CDS |
| GGAGAGTACCATGAAGTTAA+GGG | 0.402330 | 5.3:-42490649 | MS.gene053932:CDS |
| ATCATCAGTTAAGGAGCAAA+TGG | 0.407106 | 5.3:-42488957 | MS.gene053932:CDS |
| GTAGAAGCAAAGTTACATTA+AGG | 0.416879 | 5.3:-42489683 | MS.gene053932:CDS |
| TTACCAAAACACCAGAATTA+GGG | 0.422217 | 5.3:+42490713 | None:intergenic |
| AAAGAAGTGGTTGTAATTGC+TGG | 0.422244 | 5.3:-42489285 | MS.gene053932:CDS |
| TGAGAGAATGAAGTGGGAGC+AGG | 0.430736 | 5.3:-42489151 | MS.gene053932:CDS |
| GCTAAATCTGTTGCTGAAGA+TGG | 0.430828 | 5.3:-42489648 | MS.gene053932:CDS |
| GGAATTTCAAGTTAAGAAAG+TGG | 0.435170 | 5.3:-42489097 | MS.gene053932:CDS |
| TCAAACACCATCATCAGTTA+AGG | 0.444565 | 5.3:-42488966 | MS.gene053932:CDS |
| AAGGCAAACCCTTAACTTCA+TGG | 0.449187 | 5.3:+42490641 | None:intergenic |
| AAGAAAGTGGAAGAATTTGA+AGG | 0.451778 | 5.3:-42489084 | MS.gene053932:CDS |
| GAATGAAGTGGGAGCAGGAG+AGG | 0.455395 | 5.3:-42489146 | MS.gene053932:CDS |
| GACAATGGCTGATTAAGAAC+TGG | 0.473764 | 5.3:+42490532 | None:intergenic |
| ATTTGCTCCTTAACTGATGA+TGG | 0.474106 | 5.3:+42488959 | None:intergenic |
| AATGAAGTGGGAGCAGGAGA+GGG | 0.474655 | 5.3:-42489145 | MS.gene053932:CDS |
| GAAATCGAATTCTGGTTTCC+CGG | 0.474718 | 5.3:+42489524 | None:intergenic |
| GACTTCTTGTACAAAGAAAG+AGG | 0.484167 | 5.3:+42490769 | None:intergenic |
| GAATTTGAAGGGACTAATAA+GGG | 0.485288 | 5.3:-42489072 | MS.gene053932:CDS |
| GAGAGGGTTGGATGGCTTGG+TGG | 0.489813 | 5.3:-42489129 | MS.gene053932:CDS |
| TGCTGGGTGGGAAGCTATGA+TGG | 0.490086 | 5.3:-42489268 | MS.gene053932:CDS |
| TATTTGTCACTGATATCTAA+AGG | 0.492371 | 5.3:+42489729 | None:intergenic |
| TCAAGTCTTGGAGAAGCATC+AGG | 0.497137 | 5.3:-42489567 | MS.gene053932:CDS |
| TCCTTCTTTGATAAACATGA+AGG | 0.498261 | 5.3:+42489449 | None:intergenic |
| ATGTATGACATAGTATTGAT+TGG | 0.499829 | 5.3:+42490507 | None:intergenic |
| CATCTTCATGGCAAGAAAAG+AGG | 0.503885 | 5.3:-42490487 | MS.gene053932:CDS |
| TTCTGAACATCTGTTTGCTA+TGG | 0.504829 | 5.3:+42489500 | None:intergenic |
| TTTGTTATAAAGAGATTGGA+TGG | 0.511821 | 5.3:-42489012 | MS.gene053932:CDS |
| GTAGATGATTCCCTCCGTGC+CGG | 0.518251 | 5.3:-42489543 | MS.gene053932:CDS |
| AAGTGGGAGCAGGAGAGGGT+TGG | 0.525230 | 5.3:-42489141 | MS.gene053932:CDS |
| TAGATGATTCCCTCCGTGCC+GGG | 0.526204 | 5.3:-42489542 | MS.gene053932:CDS |
| CTGATTAAGAACTGGAATGA+AGG | 0.530740 | 5.3:+42490540 | None:intergenic |
| TTCTGGTTTCCCGGCACGGA+GGG | 0.530934 | 5.3:+42489533 | None:intergenic |
| GAATTAGGGCCTTCTGGTGG+AGG | 0.531123 | 5.3:+42490727 | None:intergenic |
| CGAATTCTGGTTTCCCGGCA+CGG | 0.531178 | 5.3:+42489529 | None:intergenic |
| ATGGCTTGGTGGGAAAGAAA+AGG | 0.534199 | 5.3:-42489118 | MS.gene053932:CDS |
| TTTAATAGTATCAATAATGT+AGG | 0.536802 | 5.3:-42489210 | MS.gene053932:CDS |
| AGCTATGATGGATAATAAGA+TGG | 0.539308 | 5.3:-42489256 | MS.gene053932:CDS |
| TCATCAGTTAAGGAGCAAAT+GGG | 0.542302 | 5.3:-42488956 | MS.gene053932:CDS |
| AGAGGGTTGGATGGCTTGGT+GGG | 0.548797 | 5.3:-42489128 | MS.gene053932:CDS |
| GATTACACTAGAGCAGAAAT+GGG | 0.553713 | 5.3:-42489379 | MS.gene053932:CDS |
| TTTGTACAAGAAGTCTGAAG+AGG | 0.555051 | 5.3:-42490761 | MS.gene053932:CDS |
| GGGAGCAGGAGAGGGTTGGA+TGG | 0.555243 | 5.3:-42489137 | MS.gene053932:CDS |
| CTGATCTATAAAACAACTCA+AGG | 0.565365 | 5.3:+42490602 | None:intergenic |
| ATCTTCATGGCAAGAAAAGA+GGG | 0.569344 | 5.3:-42490486 | MS.gene053932:intron |
| AGTGGTTGTAATTGCTGGGT+GGG | 0.569655 | 5.3:-42489280 | MS.gene053932:CDS |
| TTGAAGGGACTAATAAGGGA+TGG | 0.569939 | 5.3:-42489068 | MS.gene053932:CDS |
| TGTTCAGAAAGTGTTGTTGT+GGG | 0.572278 | 5.3:-42489486 | MS.gene053932:CDS |
| TAACTCAAAGGAGGAAGATG+TGG | 0.576074 | 5.3:-42489796 | MS.gene053932:CDS |
| AGCAAAGTTACATTAAGGAG+TGG | 0.577098 | 5.3:-42489678 | MS.gene053932:CDS |
| AAAATTTGGATGTTATGTGT+TGG | 0.578946 | 5.3:-42489043 | MS.gene053932:CDS |
| AGAAAGTGGAAGAATTTGAA+GGG | 0.580351 | 5.3:-42489083 | MS.gene053932:CDS |
| TATGACATAGTATTGATTGG+AGG | 0.584322 | 5.3:+42490510 | None:intergenic |
| CCTTCTTTGATAAACATGAA+GGG | 0.590946 | 5.3:+42489450 | None:intergenic |
| GTAGGAGAAAAGAACAGTGT+GGG | 0.596095 | 5.3:-42489192 | MS.gene053932:CDS |
| CTGAAGATGGATACCCTCCA+AGG | 0.601083 | 5.3:-42489635 | MS.gene053932:CDS |
| CAATAGTTGAGAGAATGAAG+TGG | 0.612002 | 5.3:-42489158 | MS.gene053932:CDS |
| ATGTTCAGAAAGTGTTGTTG+TGG | 0.614049 | 5.3:-42489487 | MS.gene053932:CDS |
| GGAAGCATACAATAACTCAA+AGG | 0.617419 | 5.3:-42489808 | MS.gene053932:CDS |
| CTCTCATTACCTCCACCAGA+AGG | 0.619265 | 5.3:-42490736 | MS.gene053932:CDS |
| CTCTCCCAACCCAAAACAAG+AGG | 0.619270 | 5.3:+42490666 | None:intergenic |
| TGTAGGAGAAAAGAACAGTG+TGG | 0.624432 | 5.3:-42489193 | MS.gene053932:CDS |
| AATAGTTGAGAGAATGAAGT+GGG | 0.629735 | 5.3:-42489157 | MS.gene053932:CDS |
| AAGAAGTGGTTGTAATTGCT+GGG | 0.631620 | 5.3:-42489284 | MS.gene053932:CDS |
| TATAGAATATGTATGTGACT+AGG | 0.634027 | 5.3:-42490792 | None:intergenic |
| AGCATACAATAACTCAAAGG+AGG | 0.645051 | 5.3:-42489805 | MS.gene053932:CDS |
| TAGTATTGATTGGAGGACAA+TGG | 0.649023 | 5.3:+42490517 | None:intergenic |
| CCAGAATTAGGGCCTTCTGG+TGG | 0.656141 | 5.3:+42490724 | None:intergenic |
| AATGTAACTTTGCTTCTACG+AGG | 0.675300 | 5.3:+42489687 | None:intergenic |
| AAGTGGTTGTAATTGCTGGG+TGG | 0.699538 | 5.3:-42489281 | MS.gene053932:CDS |
| ATTCTGGTTTCCCGGCACGG+AGG | 0.710825 | 5.3:+42489532 | None:intergenic |
| ATGATGGTGTTTGAAAGCAT+AGG | 0.759923 | 5.3:+42488975 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAATAATTTCTTAACT+TGG | - | chr5.3:42489290-42489309 | MS.gene053932:CDS | 10.0% |
| !! | TCATGTAAAAAATTAAAAAT+TGG | + | chr5.3:42489845-42489864 | None:intergenic | 10.0% |
| !! | TTTAATAGTATCAATAATGT+AGG | - | chr5.3:42490525-42490544 | MS.gene053932:CDS | 15.0% |
| !! | AGTACTATATAACTAACATT+TGG | + | chr5.3:42489348-42489367 | None:intergenic | 20.0% |
| !! | AGTATTATACTGATGTAATT+TGG | - | chr5.3:42489890-42489909 | MS.gene053932:intron | 20.0% |
| !!! | AATTAAAAATTGGTTCAACA+CGG | + | chr5.3:42489835-42489854 | None:intergenic | 20.0% |
| !!! | TAAATTTTTCGTCTGTTATT+TGG | + | chr5.3:42489501-42489520 | None:intergenic | 20.0% |
| ! | AAAATTTGGATGTTATGTGT+TGG | - | chr5.3:42490692-42490711 | MS.gene053932:CDS | 25.0% |
| ! | AAATAACAGGGAATATACTT+AGG | - | chr5.3:42489382-42489401 | MS.gene053932:CDS | 25.0% |
| ! | AATAAGGGATGGAAAAAATT+TGG | - | chr5.3:42490678-42490697 | MS.gene053932:CDS | 25.0% |
| ! | TATTTGTCACTGATATCTAA+AGG | + | chr5.3:42490009-42490028 | None:intergenic | 25.0% |
| ! | TTATATAGTACTACGACATA+AGG | - | chr5.3:42489356-42489375 | MS.gene053932:CDS | 25.0% |
| !! | TTTCTAGTATTGTAGCATAA+GGG | - | chr5.3:42489614-42489633 | MS.gene053932:CDS | 25.0% |
| !! | TTTGTTATAAAGAGATTGGA+TGG | - | chr5.3:42490723-42490742 | MS.gene053932:CDS | 25.0% |
| !!! | AATTTGGTTTTTCTTCTGTA+GGG | - | chr5.3:42489906-42489925 | MS.gene053932:intron | 25.0% |
| !!! | ATGTATGACATAGTATTGAT+TGG | + | chr5.3:42489231-42489250 | None:intergenic | 25.0% |
| !!! | GACTTTTGTTATAAAGAGAT+TGG | - | chr5.3:42490719-42490738 | MS.gene053932:CDS | 25.0% |
| !!! | GATAATGATGATGGTAAAAA+GGG | - | chr5.3:42490396-42490415 | MS.gene053932:intron | 25.0% |
| !!! | TAATTTGGTTTTTCTTCTGT+AGG | - | chr5.3:42489905-42489924 | MS.gene053932:intron | 25.0% |
| !!! | TGATAATGATGATGGTAAAA+AGG | - | chr5.3:42490395-42490414 | MS.gene053932:intron | 25.0% |
| !!! | TTTTCATGTGATAATGATGA+TGG | - | chr5.3:42490387-42490406 | MS.gene053932:intron | 25.0% |
| !!! | TTTTCTAGTATTGTAGCATA+AGG | - | chr5.3:42489613-42489632 | MS.gene053932:CDS | 25.0% |
| AAGAAAGTGGAAGAATTTGA+AGG | - | chr5.3:42490651-42490670 | MS.gene053932:CDS | 30.0% | |
| AATAGTTGAGAGAATGAAGT+GGG | - | chr5.3:42490578-42490597 | MS.gene053932:CDS | 30.0% | |
| ACATTGAATTTCGATGACAT+TGG | - | chr5.3:42489407-42489426 | MS.gene053932:CDS | 30.0% | |
| AGAAAGTGGAAGAATTTGAA+GGG | - | chr5.3:42490652-42490671 | MS.gene053932:CDS | 30.0% | |
| AGAATTTGAAGGGACTAATA+AGG | - | chr5.3:42490662-42490681 | MS.gene053932:CDS | 30.0% | |
| ATTACCAAAACACCAGAATT+AGG | + | chr5.3:42489026-42489045 | None:intergenic | 30.0% | |
| CCTTCTTTGATAAACATGAA+GGG | + | chr5.3:42490288-42490307 | None:intergenic | 30.0% | |
| CTGATATCTAAAGGATGAAT+TGG | + | chr5.3:42490000-42490019 | None:intergenic | 30.0% | |
| CTGATCTATAAAACAACTCA+AGG | + | chr5.3:42489136-42489155 | None:intergenic | 30.0% | |
| GAATTTGAAGGGACTAATAA+GGG | - | chr5.3:42490663-42490682 | MS.gene053932:CDS | 30.0% | |
| GACATAAGGTTGAAATAACA+GGG | - | chr5.3:42489370-42489389 | MS.gene053932:CDS | 30.0% | |
| GTAGAAGCAAAGTTACATTA+AGG | - | chr5.3:42490052-42490071 | MS.gene053932:intron | 30.0% | |
| GTTAGATTTGAATACAGGTT+GGG | - | chr5.3:42489750-42489769 | MS.gene053932:CDS | 30.0% | |
| TAACTAACATTTGGTTGAAC+TGG | + | chr5.3:42489339-42489358 | None:intergenic | 30.0% | |
| TACTATGTCATACATCTTCA+TGG | - | chr5.3:42489236-42489255 | MS.gene053932:CDS | 30.0% | |
| TCCTTCTTTGATAAACATGA+AGG | + | chr5.3:42490289-42490308 | None:intergenic | 30.0% | |
| TGTTAGATTTGAATACAGGT+TGG | - | chr5.3:42489749-42489768 | MS.gene053932:CDS | 30.0% | |
| TTACCAAAACACCAGAATTA+GGG | + | chr5.3:42489025-42489044 | None:intergenic | 30.0% | |
| TTCTACTTAAAAACCTTGGA+GGG | + | chr5.3:42490116-42490135 | None:intergenic | 30.0% | |
| ! | ACTTCAGTGACTTTAAGTTT+CGG | + | chr5.3:42489473-42489492 | None:intergenic | 30.0% |
| !! | AGCTATGATGGATAATAAGA+TGG | - | chr5.3:42490479-42490498 | MS.gene053932:intron | 30.0% |
| !! | CTTGAGTTGTTTTATAGATC+AGG | - | chr5.3:42489134-42489153 | MS.gene053932:CDS | 30.0% |
| !! | GGAATTTCAAGTTAAGAAAG+TGG | - | chr5.3:42490638-42490657 | MS.gene053932:CDS | 30.0% |
| !! | TATGACATAGTATTGATTGG+AGG | + | chr5.3:42489228-42489247 | None:intergenic | 30.0% |
| !! | TTGAAAACAACCTCTTGTTT+TGG | - | chr5.3:42489059-42489078 | MS.gene053932:CDS | 30.0% |
| !!! | ATGTAGCTGAAAGTTTTTTG+TGG | - | chr5.3:42490502-42490521 | MS.gene053932:CDS | 30.0% |
| !!! | GACATCAGTGTATTTTGATA+AGG | - | chr5.3:42489431-42489450 | MS.gene053932:CDS | 30.0% |
| !!! | TGAAAACAACCTCTTGTTTT+GGG | - | chr5.3:42489060-42489079 | MS.gene053932:CDS | 30.0% |
| AAAGAAGTGGTTGTAATTGC+TGG | - | chr5.3:42490450-42490469 | MS.gene053932:intron | 35.0% | |
| AAGAAGTGGTTGTAATTGCT+GGG | - | chr5.3:42490451-42490470 | MS.gene053932:intron | 35.0% | |
| AATGTAACTTTGCTTCTACG+AGG | + | chr5.3:42490051-42490070 | None:intergenic | 35.0% | |
| ACATCAAGTGATTCAAGTCT+TGG | - | chr5.3:42490156-42490175 | MS.gene053932:intron | 35.0% | |
| AGATTACACTAGAGCAGAAA+TGG | - | chr5.3:42490355-42490374 | MS.gene053932:intron | 35.0% | |
| AGCAAAGTTACATTAAGGAG+TGG | - | chr5.3:42490057-42490076 | MS.gene053932:intron | 35.0% | |
| AGCATACAATAACTCAAAGG+AGG | - | chr5.3:42489930-42489949 | MS.gene053932:intron | 35.0% | |
| ATCATCAGTTAAGGAGCAAA+TGG | - | chr5.3:42490778-42490797 | MS.gene053932:CDS | 35.0% | |
| ATCTTCATGGCAAGAAAAGA+GGG | - | chr5.3:42489249-42489268 | MS.gene053932:CDS | 35.0% | |
| ATTTGCTCCTTAACTGATGA+TGG | + | chr5.3:42490779-42490798 | None:intergenic | 35.0% | |
| CAATAGTTGAGAGAATGAAG+TGG | - | chr5.3:42490577-42490596 | MS.gene053932:CDS | 35.0% | |
| CACCTTCTACTTAAAAACCT+TGG | + | chr5.3:42490120-42490139 | None:intergenic | 35.0% | |
| CCCTTCATGTTTATCAAAGA+AGG | - | chr5.3:42490285-42490304 | MS.gene053932:intron | 35.0% | |
| CGACATAAGGTTGAAATAAC+AGG | - | chr5.3:42489369-42489388 | MS.gene053932:CDS | 35.0% | |
| CTGATTAAGAACTGGAATGA+AGG | + | chr5.3:42489198-42489217 | None:intergenic | 35.0% | |
| CTTAAGGGTGGAATTAAAGA+TGG | - | chr5.3:42489546-42489565 | MS.gene053932:CDS | 35.0% | |
| CTTCTACTTAAAAACCTTGG+AGG | + | chr5.3:42490117-42490136 | None:intergenic | 35.0% | |
| GAAACTTAAAGTCACTGAAG+TGG | - | chr5.3:42489472-42489491 | MS.gene053932:CDS | 35.0% | |
| GACTTCTTGTACAAAGAAAG+AGG | + | chr5.3:42488969-42488988 | None:intergenic | 35.0% | |
| GAGCTGTTAGATTTGAATAC+AGG | - | chr5.3:42489745-42489764 | MS.gene053932:CDS | 35.0% | |
| GATTACACTAGAGCAGAAAT+GGG | - | chr5.3:42490356-42490375 | MS.gene053932:intron | 35.0% | |
| TCAAACACCATCATCAGTTA+AGG | - | chr5.3:42490769-42490788 | MS.gene053932:CDS | 35.0% | |
| TCATCAGTTAAGGAGCAAAT+GGG | - | chr5.3:42490779-42490798 | MS.gene053932:CDS | 35.0% | |
| TGCTGTTGTTCAAAAAGAAG+TGG | - | chr5.3:42490437-42490456 | MS.gene053932:intron | 35.0% | |
| TTAAACTCGATCATCAAAGC+AGG | - | chr5.3:42489518-42489537 | MS.gene053932:CDS | 35.0% | |
| TTTGTACAAGAAGTCTGAAG+AGG | - | chr5.3:42488974-42488993 | MS.gene053932:CDS | 35.0% | |
| ! | AACTCAAGGTTCATGTTTTG+AGG | + | chr5.3:42489122-42489141 | None:intergenic | 35.0% |
| ! | ATGATGGTGTTTGAAAGCAT+AGG | + | chr5.3:42490763-42490782 | None:intergenic | 35.0% |
| ! | GGAAGCATACAATAACTCAA+AGG | - | chr5.3:42489927-42489946 | MS.gene053932:intron | 35.0% |
| ! | TTCTGAACATCTGTTTGCTA+TGG | + | chr5.3:42490238-42490257 | None:intergenic | 35.0% |
| !! | AAAGTGTTGTTGTGGGAAAA+TGG | - | chr5.3:42490256-42490275 | MS.gene053932:intron | 35.0% |
| !! | ACTCAAGGTTCATGTTTTGA+GGG | + | chr5.3:42489121-42489140 | None:intergenic | 35.0% |
| !! | ATGTTCAGAAAGTGTTGTTG+TGG | - | chr5.3:42490248-42490267 | MS.gene053932:intron | 35.0% |
| !! | TAGTATTGATTGGAGGACAA+TGG | + | chr5.3:42489221-42489240 | None:intergenic | 35.0% |
| !! | TGTTCAGAAAGTGTTGTTGT+GGG | - | chr5.3:42490249-42490268 | MS.gene053932:intron | 35.0% |
| !!! | CAAGGTTTTTAAGTAGAAGG+TGG | - | chr5.3:42490118-42490137 | MS.gene053932:intron | 35.0% |
| !!! | CTCCAAGGTTTTTAAGTAGA+AGG | - | chr5.3:42490115-42490134 | MS.gene053932:intron | 35.0% |
| AAGGCAAACCCTTAACTTCA+TGG | + | chr5.3:42489097-42489116 | None:intergenic | 40.0% | |
| CATCTTCATGGCAAGAAAAG+AGG | - | chr5.3:42489248-42489267 | MS.gene053932:CDS | 40.0% | |
| GACAATGGCTGATTAAGAAC+TGG | + | chr5.3:42489206-42489225 | None:intergenic | 40.0% | |
| GCTAAATCTGTTGCTGAAGA+TGG | - | chr5.3:42490087-42490106 | MS.gene053932:intron | 40.0% | |
| GCTATGGTGAAATCGAATTC+TGG | + | chr5.3:42490222-42490241 | None:intergenic | 40.0% | |
| GTAGGAGAAAAGAACAGTGT+GGG | - | chr5.3:42490543-42490562 | MS.gene053932:CDS | 40.0% | |
| TAACTCAAAGGAGGAAGATG+TGG | - | chr5.3:42489939-42489958 | MS.gene053932:intron | 40.0% | |
| TATCTCTATTACCCTGAACG+TGG | + | chr5.3:42489811-42489830 | None:intergenic | 40.0% | |
| TCATGCACATGTACTCATCA+TGG | + | chr5.3:42489683-42489702 | None:intergenic | 40.0% | |
| TGTAGGAGAAAAGAACAGTG+TGG | - | chr5.3:42490542-42490561 | MS.gene053932:CDS | 40.0% | |
| TTGAAGGGACTAATAAGGGA+TGG | - | chr5.3:42490667-42490686 | MS.gene053932:CDS | 40.0% | |
| !! | GAAATCGAATTCTGGTTTCC+CGG | + | chr5.3:42490214-42490233 | None:intergenic | 40.0% |
| !! | GGAGAGTACCATGAAGTTAA+GGG | - | chr5.3:42489086-42489105 | MS.gene053932:CDS | 40.0% |
| !!! | AACAACCTCTTGTTTTGGGT+TGG | - | chr5.3:42489064-42489083 | MS.gene053932:CDS | 40.0% |
| !!! | ACAACCTCTTGTTTTGGGTT+GGG | - | chr5.3:42489065-42489084 | MS.gene053932:CDS | 40.0% |
| !!! | AGGTTCATGTTTTGAGGGAA+AGG | + | chr5.3:42489116-42489135 | None:intergenic | 40.0% |
| AAGTGGTTGTAATTGCTGGG+TGG | - | chr5.3:42490454-42490473 | MS.gene053932:intron | 45.0% | |
| AGTGGTTGTAATTGCTGGGT+GGG | - | chr5.3:42490455-42490474 | MS.gene053932:intron | 45.0% | |
| ATCAAAGCAGGCGTCACTTA+AGG | - | chr5.3:42489530-42489549 | MS.gene053932:CDS | 45.0% | |
| GAACAGTGTGGGTTTGAGTA+TGG | - | chr5.3:42490554-42490573 | MS.gene053932:CDS | 45.0% | |
| TCAAAGCAGGCGTCACTTAA+GGG | - | chr5.3:42489531-42489550 | MS.gene053932:CDS | 45.0% | |
| ! | AGGCCCTAATTCTGGTGTTT+TGG | - | chr5.3:42489019-42489038 | MS.gene053932:CDS | 45.0% |
| !! | ATGGCTTGGTGGGAAAGAAA+AGG | - | chr5.3:42490617-42490636 | MS.gene053932:CDS | 45.0% |
| !! | GGGAGAGTACCATGAAGTTA+AGG | - | chr5.3:42489085-42489104 | MS.gene053932:CDS | 45.0% |
| !! | TCAAGTCTTGGAGAAGCATC+AGG | - | chr5.3:42490168-42490187 | MS.gene053932:intron | 45.0% |
| AACTCTGTTGCACGATGCAG+CGG | - | chr5.3:42489716-42489735 | MS.gene053932:CDS | 50.0% | |
| AATGAAGTGGGAGCAGGAGA+GGG | - | chr5.3:42490590-42490609 | MS.gene053932:CDS | 50.0% | |
| ACACCAGAATTAGGGCCTTC+TGG | + | chr5.3:42489017-42489036 | None:intergenic | 50.0% | |
| CTCTCATTACCTCCACCAGA+AGG | - | chr5.3:42488999-42489018 | MS.gene053932:CDS | 50.0% | |
| CTCTCCCAACCCAAAACAAG+AGG | + | chr5.3:42489072-42489091 | None:intergenic | 50.0% | |
| CTGAAGATGGATACCCTCCA+AGG | - | chr5.3:42490100-42490119 | MS.gene053932:intron | 50.0% | |
| GCAGCGAGTGCTATAACTGT+TGG | + | chr5.3:42489789-42489808 | None:intergenic | 50.0% | |
| GCATCGTGCAACAGAGTTAG+AGG | + | chr5.3:42489714-42489733 | None:intergenic | 50.0% | |
| TGAGAGAATGAAGTGGGAGC+AGG | - | chr5.3:42490584-42490603 | MS.gene053932:CDS | 50.0% | |
| AAGCAGGCGTCACTTAAGGG+TGG | - | chr5.3:42489534-42489553 | MS.gene053932:CDS | 55.0% | |
| CCACCAGAAGGCCCTAATTC+TGG | - | chr5.3:42489011-42489030 | MS.gene053932:CDS | 55.0% | |
| CCAGAATTAGGGCCTTCTGG+TGG | + | chr5.3:42489014-42489033 | None:intergenic | 55.0% | |
| GAATGAAGTGGGAGCAGGAG+AGG | - | chr5.3:42490589-42490608 | MS.gene053932:CDS | 55.0% | |
| GAATTAGGGCCTTCTGGTGG+AGG | + | chr5.3:42489011-42489030 | None:intergenic | 55.0% | |
| GTAGATGATTCCCTCCGTGC+CGG | - | chr5.3:42490192-42490211 | MS.gene053932:intron | 55.0% | |
| TAGATGATTCCCTCCGTGCC+GGG | - | chr5.3:42490193-42490212 | MS.gene053932:intron | 55.0% | |
| TGCTGGGTGGGAAGCTATGA+TGG | - | chr5.3:42490467-42490486 | MS.gene053932:intron | 55.0% | |
| !! | AGAGGGTTGGATGGCTTGGT+GGG | - | chr5.3:42490607-42490626 | MS.gene053932:CDS | 55.0% |
| !! | CGAATTCTGGTTTCCCGGCA+CGG | + | chr5.3:42490209-42490228 | None:intergenic | 55.0% |
| AAGTGGGAGCAGGAGAGGGT+TGG | - | chr5.3:42490594-42490613 | MS.gene053932:CDS | 60.0% | |
| AGCACTCGCTGCCACGTTCA+GGG | - | chr5.3:42489797-42489816 | MS.gene053932:CDS | 60.0% | |
| CAGGAGAGGGTTGGATGGCT+TGG | - | chr5.3:42490603-42490622 | MS.gene053932:CDS | 60.0% | |
| TAGCACTCGCTGCCACGTTC+AGG | - | chr5.3:42489796-42489815 | MS.gene053932:CDS | 60.0% | |
| ! | GAGAGGGTTGGATGGCTTGG+TGG | - | chr5.3:42490606-42490625 | MS.gene053932:CDS | 60.0% |
| !! | ATTCTGGTTTCCCGGCACGG+AGG | + | chr5.3:42490206-42490225 | None:intergenic | 60.0% |
| !! | TTCTGGTTTCCCGGCACGGA+GGG | + | chr5.3:42490205-42490224 | None:intergenic | 60.0% |
| GGGAGCAGGAGAGGGTTGGA+TGG | - | chr5.3:42490598-42490617 | MS.gene053932:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 42488951 | 42490806 | 42488951 | ID=MS.gene053932 |
| chr5.3 | mRNA | 42488951 | 42490806 | 42488951 | ID=MS.gene053932.t1;Parent=MS.gene053932 |
| chr5.3 | exon | 42490487 | 42490806 | 42490487 | ID=MS.gene053932.t1.exon1;Parent=MS.gene053932.t1 |
| chr5.3 | CDS | 42490487 | 42490806 | 42490487 | ID=cds.MS.gene053932.t1;Parent=MS.gene053932.t1 |
| chr5.3 | exon | 42488951 | 42489830 | 42488951 | ID=MS.gene053932.t1.exon2;Parent=MS.gene053932.t1 |
| chr5.3 | CDS | 42488951 | 42489830 | 42488951 | ID=cds.MS.gene053932.t1;Parent=MS.gene053932.t1 |
| Gene Sequence |
| Protein sequence |