AlfalfaGEDB | MS.gene57465

M. sativa cultivar XinJiangDaYe / MS.gene57465

KEGG: -
GO: -
PFAM: PF05678.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57465.t1	XP_003594889.2	96.6	237	7	1	1	236	1	237	1.20E-122	449.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57465.t1	Q9LIE6	49.3	75	37	1	63	136	42	116	5.5e-10	66.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57465.t1	G7INF7	96.6	237	7	1	1	236	1	237	9.0e-123	449.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052053	MS.gene57465	0.820955	5.22E-53	-1.69E-46
MS.gene052091	MS.gene57465	0.815173	1.06E-51	-1.69E-46
MS.gene053932	MS.gene57465	0.803044	4.21E-49	-1.69E-46
MS.gene060326	MS.gene57465	0.853576	2.18E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57465.t1	MTR_2g035850	96.624	237	7	1	1	236	1	237	1.31e-169	467
MS.gene57465.t1	MTR_4g094698	49.254	134	45	5	1	131	1	114	5.01e-32	115

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 43 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTACCTTGTTGAAAGTTTA+AGG	0.076288	2.2:+50743155	None:intergenic
TCATCACATAAAAGTATTAT+TGG	0.207412	2.2:+50742840	None:intergenic
GCTCTAGATCTTCTTCTAAT+TGG	0.209397	2.2:+50743312	None:intergenic
TTGCACAAGTTGTCTAAAAT+TGG	0.266497	2.2:+50743248	None:intergenic
GATAGTATTGCATCCAATAT+TGG	0.285397	2.2:+50743502	None:intergenic
TTGGTTGTGGATAATGATTT+TGG	0.308541	2.2:-50742892	MS.gene57465:CDS
TGTTGAAAGTTTAAGGTAAT+AGG	0.323637	2.2:+50743162	None:intergenic
CTGCTTCTACTGACCAATAT+TGG	0.333962	2.2:-50743515	MS.gene57465:CDS
AGATCTTCTTCTAATTGGTT+TGG	0.337371	2.2:+50743317	None:intergenic
CTCAAAATCATAGTCTCTAA+TGG	0.337812	2.2:+50743399	None:intergenic
TGCTCCTTGTTCCATGGAAA+TGG	0.342079	2.2:+50743039	None:intergenic
CTTGGATGTTTCTGATGGTT+TGG	0.350629	2.2:-50742911	MS.gene57465:CDS
GACGACGAGTTACCCGAATT+CGG	0.392141	2.2:+50742946	None:intergenic
TTGGATATATTACCACCCTT+TGG	0.400197	2.2:+50743336	None:intergenic
ATCATAGTCTCTAATGGAAC+AGG	0.408085	2.2:+50743405	None:intergenic
GTGTTTGTGTATGAATTTGT+AGG	0.408854	2.2:+50743375	None:intergenic
TACCACCCTTTGGTGTCAAT+TGG	0.410144	2.2:+50743346	None:intergenic
TTTGCATTAAGTAGTGTTGT+TGG	0.420896	2.2:+50743276	None:intergenic
GTCGTCAAGGCCTAGTAACT+TGG	0.429524	2.2:-50742929	MS.gene57465:CDS
TGTTTCTGATGGTTTGGTTG+TGG	0.449182	2.2:-50742905	MS.gene57465:CDS
TGAATTTGCTCCTTGTTCCA+TGG	0.471507	2.2:+50743033	None:intergenic
TTGTCCTCGTCGTCGTCGCT+AGG	0.494806	2.2:-50743198	MS.gene57465:CDS
ATACTATCAACAACCTCTCA+TGG	0.500539	2.2:-50743487	MS.gene57465:CDS
AGTAACTTGGATGTTTCTGA+TGG	0.501901	2.2:-50742916	MS.gene57465:CDS
TCGTCGCTAGGTGTTCATAA+AGG	0.509070	2.2:-50743186	MS.gene57465:CDS
TGTTTGTGTATGAATTTGTA+GGG	0.517144	2.2:+50743376	None:intergenic
GACGAGGACAAAGCTGTACT+AGG	0.537020	2.2:+50743210	None:intergenic
TCAGAAACATCCAAGTTACT+AGG	0.537297	2.2:+50742919	None:intergenic
AGTGACCAATTGACACCAAA+GGG	0.545409	2.2:-50743351	MS.gene57465:CDS
CAACTTGTGCAACAATTCAC+AGG	0.545584	2.2:-50743237	MS.gene57465:CDS
AGGAGCAAATTCAAGCGCCT+CGG	0.584501	2.2:-50743023	MS.gene57465:CDS
TAACCACTCTGCTGTTGCCG+AGG	0.590379	2.2:+50743006	None:intergenic
TTGGTCAGTAGAAGCAGTCA+TGG	0.595873	2.2:+50743521	None:intergenic
AAGTGACTAAGCCATTTCCA+TGG	0.609099	2.2:-50743050	MS.gene57465:CDS
TAGTGACCAATTGACACCAA+AGG	0.616846	2.2:-50743352	MS.gene57465:CDS
AACACCTAGCGACGACGACG+AGG	0.626702	2.2:+50743194	None:intergenic
ATTCGGGTAACTCGTCGTCA+AGG	0.628642	2.2:-50742942	MS.gene57465:CDS
TAAGCCATTTCCATGGAACA+AGG	0.637693	2.2:-50743043	MS.gene57465:CDS
TTCAATCCATAACACATGAG+TGG	0.650240	2.2:-50743546	None:intergenic
TTTAGAGTATGATCCATGAG+AGG	0.654825	2.2:+50743474	None:intergenic
ATTACCTTAAACTTTCAACA+AGG	0.690320	2.2:-50743159	MS.gene57465:CDS
GACCAATTGACACCAAAGGG+TGG	0.701501	2.2:-50743348	MS.gene57465:CDS
GCGCCTCGGCAACAGCAGAG+TGG	0.703474	2.2:-50743009	MS.gene57465:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTACCTTAAACTTTCAACA+AGG	-	chr2.2:50743215-50743234	MS.gene57465:CDS	25.0%
!	TGTTTGTGTATGAATTTGTA+GGG	+	chr2.2:50743001-50743020	None:intergenic	25.0%
!!	GAATCAGAGAAATTTTCTAT+AGG	+	chr2.2:50742930-50742949	None:intergenic	25.0%
!!	TGTTGAAAGTTTAAGGTAAT+AGG	+	chr2.2:50743215-50743234	None:intergenic	25.0%
	ACTACCTTGTTGAAAGTTTA+AGG	+	chr2.2:50743222-50743241	None:intergenic	30.0%
	AGATCTTCTTCTAATTGGTT+TGG	+	chr2.2:50743060-50743079	None:intergenic	30.0%
	CTCAAAATCATAGTCTCTAA+TGG	+	chr2.2:50742978-50742997	None:intergenic	30.0%
	GATAGTATTGCATCCAATAT+TGG	+	chr2.2:50742875-50742894	None:intergenic	30.0%
	GTGTTTGTGTATGAATTTGT+AGG	+	chr2.2:50743002-50743021	None:intergenic	30.0%
	TTTGCATTAAGTAGTGTTGT+TGG	+	chr2.2:50743101-50743120	None:intergenic	30.0%
!	AACAATTTTGTTCCGAATTC+GGG	-	chr2.2:50743416-50743435	MS.gene57465:CDS	30.0%
!	CAACAATTTTGTTCCGAATT+CGG	-	chr2.2:50743415-50743434	MS.gene57465:CDS	30.0%
!	TCAGAGAAATTTTCTATAGG+TGG	+	chr2.2:50742927-50742946	None:intergenic	30.0%
!	TTGCACAAGTTGTCTAAAAT+TGG	+	chr2.2:50743129-50743148	None:intergenic	30.0%
!	TTGGTTGTGGATAATGATTT+TGG	-	chr2.2:50743482-50743501	MS.gene57465:CDS	30.0%
	ATACTATCAACAACCTCTCA+TGG	-	chr2.2:50742887-50742906	MS.gene57465:CDS	35.0%
	ATCATAGTCTCTAATGGAAC+AGG	+	chr2.2:50742972-50742991	None:intergenic	35.0%
	TCAGAAACATCCAAGTTACT+AGG	+	chr2.2:50743458-50743477	None:intergenic	35.0%
	TTGGATATATTACCACCCTT+TGG	+	chr2.2:50743041-50743060	None:intergenic	35.0%
!	AGTAACTTGGATGTTTCTGA+TGG	-	chr2.2:50743458-50743477	MS.gene57465:CDS	35.0%
!	GCTCTAGATCTTCTTCTAAT+TGG	+	chr2.2:50743065-50743084	None:intergenic	35.0%
!	TTTAGAGTATGATCCATGAG+AGG	+	chr2.2:50742903-50742922	None:intergenic	35.0%
	AAGTGACTAAGCCATTTCCA+TGG	-	chr2.2:50743324-50743343	MS.gene57465:CDS	40.0%
	AGTGACCAATTGACACCAAA+GGG	-	chr2.2:50743023-50743042	MS.gene57465:CDS	40.0%
	CAACTTGTGCAACAATTCAC+AGG	-	chr2.2:50743137-50743156	MS.gene57465:CDS	40.0%
	CTGCTTCTACTGACCAATAT+TGG	-	chr2.2:50742859-50742878	MS.gene57465:CDS	40.0%
	TAAGCCATTTCCATGGAACA+AGG	-	chr2.2:50743331-50743350	MS.gene57465:CDS	40.0%
	TAGTGACCAATTGACACCAA+AGG	-	chr2.2:50743022-50743041	MS.gene57465:CDS	40.0%
!	CTTGGATGTTTCTGATGGTT+TGG	-	chr2.2:50743463-50743482	MS.gene57465:CDS	40.0%
!	TGAATTTGCTCCTTGTTCCA+TGG	+	chr2.2:50743344-50743363	None:intergenic	40.0%
!!	TGTTTCTGATGGTTTGGTTG+TGG	-	chr2.2:50743469-50743488	MS.gene57465:CDS	40.0%
	TCGTCGCTAGGTGTTCATAA+AGG	-	chr2.2:50743188-50743207	MS.gene57465:CDS	45.0%
	TGCTCCTTGTTCCATGGAAA+TGG	+	chr2.2:50743338-50743357	None:intergenic	45.0%
	TTGGTCAGTAGAAGCAGTCA+TGG	+	chr2.2:50742856-50742875	None:intergenic	45.0%
!!	TACCACCCTTTGGTGTCAAT+TGG	+	chr2.2:50743031-50743050	None:intergenic	45.0%
	AGGAGCAAATTCAAGCGCCT+CGG	-	chr2.2:50743351-50743370	MS.gene57465:CDS	50.0%
	ATTCGGGTAACTCGTCGTCA+AGG	-	chr2.2:50743432-50743451	MS.gene57465:CDS	50.0%
	GACCAATTGACACCAAAGGG+TGG	-	chr2.2:50743026-50743045	MS.gene57465:CDS	50.0%
	GACGACGAGTTACCCGAATT+CGG	+	chr2.2:50743431-50743450	None:intergenic	50.0%
	GACGAGGACAAAGCTGTACT+AGG	+	chr2.2:50743167-50743186	None:intergenic	50.0%
	GTCGTCAAGGCCTAGTAACT+TGG	-	chr2.2:50743445-50743464	MS.gene57465:CDS	50.0%
	TAACCACTCTGCTGTTGCCG+AGG	+	chr2.2:50743371-50743390	None:intergenic	55.0%
	AACACCTAGCGACGACGACG+AGG	+	chr2.2:50743183-50743202	None:intergenic	60.0%
	TTGTCCTCGTCGTCGTCGCT+AGG	-	chr2.2:50743176-50743195	MS.gene57465:CDS	60.0%
	GCGCCTCGGCAACAGCAGAG+TGG	-	chr2.2:50743365-50743384	MS.gene57465:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	50742843	50743553	50742843	ID=MS.gene57465
chr2.2	mRNA	50742843	50743553	50742843	ID=MS.gene57465.t1;Parent=MS.gene57465
chr2.2	exon	50742843	50743553	50742843	ID=MS.gene57465.t1.exon1;Parent=MS.gene57465.t1
chr2.2	CDS	50742843	50743553	50742843	ID=cds.MS.gene57465.t1;Parent=MS.gene57465.t1

Gene Sequence

>MS.gene57465

ATGAGTGGTACCATGACTGCTTCTACTGACCAATATTGGATGCAATACTATCAACAACCTCTCATGGATCATACTCTAAATCCACCTATAGAAAATTTCTCTGATTCAACTGTGATGACAACAAATCCTGTTCCATTAGAGACTATGATTTTGAGCCCTACAAATTCATACACAAACACTAGTGACCAATTGACACCAAAGGGTGGTAATATATCCAAACCAATTAGAAGAAGATCTAGAGCTTCTAAGAGAACACCAACAACACTACTTAATGCAAACACAACCAATTTTAGACAACTTGTGCAACAATTCACAGGTTGTCCTAGTACAGCTTTGTCCTCGTCGTCGTCGCTAGGTGTTCATAAAGGACCTATTACCTTAAACTTTCAACAAGGTAGTAGCAAACAGAATGTTCAACATGACACAACAGCTAGATTAATGACACAACTTAGTAGCACAAGTTACAATCAAGTTCATCATCAAGTGACTAAGCCATTTCCATGGAACAAGGAGCAAATTCAAGCGCCTCGGCAACAGCAGAGTGGTTATAACTCATTTGAATATGTCAAGAACAACAATTTTGTTCCGAATTCGGGTAACTCGTCGTCAAGGCCTAGTAACTTGGATGTTTCTGATGGTTTGGTTGTGGATAATGATTTTGGTTTGCATGATCTAACTGTTAATGAATTCTCCAATAATACTTTTATGTGA

Protein sequence

>MS.gene57465.t1

MSGTMTASTDQYWMQYYQQPLMDHTLNPPIENFSDSTVMTTNPVPLETMILSPTNSYTNTSDQLTPKGGNISKPIRRRSRASKRTPTTLLNANTTNFRQLVQQFTGCPSTALSSSSSLGVHKGPITLNFQQGSSKQNVQHDTTARLMTQLSSTSYNQVHHQVTKPFPWNKEQIQAPRQQQSGYNSFEYVKNNNFVPNSGNSSSRPSNLDVSDGLVVDNDFGLHDLTVNEFSNNTFM