Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57646.t1 | XP_013458483.1 | 98.2 | 220 | 4 | 0 | 1 | 220 | 203 | 422 | 1.20E-122 | 449.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57646.t1 | Q9LTS3 | 53.6 | 222 | 95 | 2 | 1 | 219 | 205 | 421 | 2.5e-65 | 250.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57646.t1 | A0A072URS8 | 98.2 | 220 | 4 | 0 | 1 | 220 | 203 | 422 | 8.4e-123 | 449.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05104 | MS.gene57646 | 0.805566 | 1.25E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene57646 | 0.809152 | 2.18E-50 | -1.69E-46 |
| MS.gene051863 | MS.gene57646 | 0.909329 | 6.43E-82 | -1.69E-46 |
| MS.gene053488 | MS.gene57646 | 0.801937 | 7.11E-49 | -1.69E-46 |
| MS.gene058093 | MS.gene57646 | 0.815434 | 9.26E-52 | -1.69E-46 |
| MS.gene058099 | MS.gene57646 | 0.831088 | 2.03E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGGTCTAGGCCAATT+TGG | 0.234892 | 8.4:-8706266 | MS.gene57646:CDS |
| ATGCGAGGTTGATCAATTTC+TGG | 0.237168 | 8.4:+8705621 | None:intergenic |
| TCAATGAGATTAAATGTTCT+TGG | 0.259196 | 8.4:+8705727 | None:intergenic |
| TGATGTAGATTATGCCATAT+TGG | 0.273247 | 8.4:+8705583 | None:intergenic |
| GAACATGTTACTTACCCTTT+TGG | 0.283606 | 8.4:+8706205 | None:intergenic |
| ACTTACTTTGTTCGGTTCAT+TGG | 0.299489 | 8.4:+8705117 | None:intergenic |
| TTGGAGGTCTAGGCCAATTT+GGG | 0.311083 | 8.4:-8706265 | MS.gene57646:CDS |
| CCCGGGTTATAATCCCAAAT+TGG | 0.317668 | 8.4:+8706252 | None:intergenic |
| TGGTTAGACCTATTTGTTCC+AGG | 0.317776 | 8.4:-8705225 | MS.gene57646:CDS |
| CACTAAGGGCACAAGGGCTT+TGG | 0.322463 | 8.4:-8705263 | MS.gene57646:CDS |
| GATGTAGATTATGCCATATT+GGG | 0.334379 | 8.4:+8705584 | None:intergenic |
| ACTTCCTTGGTAACCCAATA+TGG | 0.343635 | 8.4:-8705597 | MS.gene57646:CDS |
| AATGCACCTAATTATGTAGA+AGG | 0.361571 | 8.4:-8705687 | MS.gene57646:CDS |
| CTTACTTTGTTCGGTTCATT+GGG | 0.402378 | 8.4:+8705118 | None:intergenic |
| CTCTCAAGATCATGTTGATC+AGG | 0.409029 | 8.4:-8705532 | MS.gene57646:intron |
| GAACATTTAATCTCATTGAA+TGG | 0.419334 | 8.4:-8705723 | MS.gene57646:CDS |
| TATGCGGTTCTTGGAGGTCT+AGG | 0.420552 | 8.4:-8706275 | MS.gene57646:CDS |
| ACTAAGGGCACAAGGGCTTT+GGG | 0.426367 | 8.4:-8705262 | MS.gene57646:CDS |
| CTCTTGGACCTGCACCCAAA+AGG | 0.431378 | 8.4:-8706220 | MS.gene57646:CDS |
| GTCCAAGAGCTATTCTTGCC+CGG | 0.443689 | 8.4:+8706234 | None:intergenic |
| TTTGGGATATCCCACATCCT+TGG | 0.453084 | 8.4:-8705245 | MS.gene57646:CDS |
| AATTCAGACATTCTAGATCC+TGG | 0.456400 | 8.4:+8705207 | None:intergenic |
| AGAAGTCGTTGTAAAGTAAA+CGG | 0.480233 | 8.4:+8705766 | None:intergenic |
| ATTTAATGAAGGTGTGTTCA+AGG | 0.481817 | 8.4:-8705187 | MS.gene57646:CDS |
| TTCTAGATCCTGGAACAAAT+AGG | 0.484678 | 8.4:+8705217 | None:intergenic |
| TTAATTTCTTGTAGGTAAAG+TGG | 0.485135 | 8.4:-8705791 | MS.gene57646:intron |
| TCAACCTCGCATAACTTCCT+TGG | 0.491369 | 8.4:-8705610 | MS.gene57646:CDS |
| TCTTGGACCTGCACCCAAAA+GGG | 0.495846 | 8.4:-8706219 | MS.gene57646:intron |
| AATAGGTCTAACCAAGGATG+TGG | 0.503374 | 8.4:+8705234 | None:intergenic |
| ACCCGGGCAAGAATAGCTCT+TGG | 0.507789 | 8.4:-8706236 | MS.gene57646:CDS |
| GCCAATTTGGGATTATAACC+CGG | 0.514228 | 8.4:-8706253 | MS.gene57646:CDS |
| TCCAAGAGCTATTCTTGCCC+GGG | 0.517850 | 8.4:+8706235 | None:intergenic |
| CCAATTTGGGATTATAACCC+GGG | 0.522205 | 8.4:-8706252 | MS.gene57646:CDS |
| TTTCATAGGGAAAGGAGACT+TGG | 0.523319 | 8.4:-8706333 | None:intergenic |
| AGAATGTCTGAATTTAATGA+AGG | 0.553370 | 8.4:-8705198 | MS.gene57646:CDS |
| CTTAAACAAAACATTACAGC+AGG | 0.556213 | 8.4:-8705156 | MS.gene57646:CDS |
| TTTAATGAAGGTGTGTTCAA+GGG | 0.557994 | 8.4:-8705186 | MS.gene57646:CDS |
| TACTGATGAGCTAACACTAA+GGG | 0.595369 | 8.4:-8705277 | MS.gene57646:CDS |
| ATACTGATGAGCTAACACTA+AGG | 0.611848 | 8.4:-8705278 | MS.gene57646:CDS |
| GAGCTAACACTAAGGGCACA+AGG | 0.614915 | 8.4:-8705270 | MS.gene57646:CDS |
| TATGCCATATTGGGTTACCA+AGG | 0.624328 | 8.4:+8705593 | None:intergenic |
| AGCTAACACTAAGGGCACAA+GGG | 0.649584 | 8.4:-8705269 | MS.gene57646:CDS |
| ATAGGTCTAACCAAGGATGT+GGG | 0.663453 | 8.4:+8705235 | None:intergenic |
| GGAACAAATAGGTCTAACCA+AGG | 0.693806 | 8.4:+8705228 | None:intergenic |
| GTTACCAAGGAAGTTATGCG+AGG | 0.707702 | 8.4:+8705606 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATTTACAAAAATTTA+AGG | + | chr8.4:8705406-8705425 | None:intergenic | 10.0% |
| !! | GTTTAATTAGTAATTTAATT+TGG | + | chr8.4:8705627-8705646 | None:intergenic | 10.0% |
| !!! | TTGTAAATTCTTTTATTATA+GGG | - | chr8.4:8705413-8705432 | MS.gene57646:intron | 10.0% |
| !!! | TTTGTAAATTCTTTTATTAT+AGG | - | chr8.4:8705412-8705431 | MS.gene57646:intron | 10.0% |
| !! | AATATATTTACTTATTCGTA+TGG | - | chr8.4:8705273-8705292 | MS.gene57646:CDS | 15.0% |
| !! | AAAAAACCTTCTACATAATT+AGG | + | chr8.4:8705770-8705789 | None:intergenic | 20.0% |
| !! | CAAATGTAGAAATAATGTAT+TGG | + | chr8.4:8705556-8705575 | None:intergenic | 20.0% |
| !! | TTGTTTGATTAATTTCTTGT+AGG | - | chr8.4:8705649-8705668 | MS.gene57646:CDS | 20.0% |
| !!! | AGTAATTTAATTTGGGATAA+AGG | + | chr8.4:8705619-8705638 | None:intergenic | 20.0% |
| !!! | CAATACATTATTTCTACATT+TGG | - | chr8.4:8705554-8705573 | MS.gene57646:CDS | 20.0% |
| !!! | CTTTTTCGAATTTAAAAGTA+GGG | + | chr8.4:8706117-8706136 | None:intergenic | 20.0% |
| !!! | GTAATTTAATTTGGGATAAA+GGG | + | chr8.4:8705618-8705637 | None:intergenic | 20.0% |
| !!! | TCTTTTTCGAATTTAAAAGT+AGG | + | chr8.4:8706118-8706137 | None:intergenic | 20.0% |
| ! | AATTGAATGCACAAAGTTTA+AGG | + | chr8.4:8705496-8705515 | None:intergenic | 25.0% |
| ! | AGAATGTCTGAATTTAATGA+AGG | - | chr8.4:8706250-8706269 | MS.gene57646:CDS | 25.0% |
| ! | ATTGAATGCACAAAGTTTAA+GGG | + | chr8.4:8705495-8705514 | None:intergenic | 25.0% |
| ! | GAACATTTAATCTCATTGAA+TGG | - | chr8.4:8705725-8705744 | MS.gene57646:CDS | 25.0% |
| ! | TCAATGAGATTAAATGTTCT+TGG | + | chr8.4:8705724-8705743 | None:intergenic | 25.0% |
| ! | TTAATTTCTTGTAGGTAAAG+TGG | - | chr8.4:8705657-8705676 | MS.gene57646:CDS | 25.0% |
| !! | GAAATTGAAATTTTGCTTCA+GGG | - | chr8.4:8706079-8706098 | MS.gene57646:intron | 25.0% |
| !! | TTGATCAATTTCTGGAAAAA+AGG | + | chr8.4:8705822-8705841 | None:intergenic | 25.0% |
| !!! | GAACTCAGAATTATTTTATG+CGG | - | chr8.4:8705157-8705176 | MS.gene57646:CDS | 25.0% |
| !!! | TATCGTTTTGTTTCTTGAAT+AGG | - | chr8.4:8706057-8706076 | MS.gene57646:intron | 25.0% |
| AATGCACCTAATTATGTAGA+AGG | - | chr8.4:8705761-8705780 | MS.gene57646:CDS | 30.0% | |
| AGAAGTCGTTGTAAAGTAAA+CGG | + | chr8.4:8705685-8705704 | None:intergenic | 30.0% | |
| CTTAAACAAAACATTACAGC+AGG | - | chr8.4:8706292-8706311 | MS.gene57646:CDS | 30.0% | |
| GATGTAGATTATGCCATATT+GGG | + | chr8.4:8705867-8705886 | None:intergenic | 30.0% | |
| TGAAAATAGCATGACTACTT+TGG | + | chr8.4:8705995-8706014 | None:intergenic | 30.0% | |
| TGATGTAGATTATGCCATAT+TGG | + | chr8.4:8705868-8705887 | None:intergenic | 30.0% | |
| TTTCTAAACTTGTGCCTTAA+GGG | - | chr8.4:8705577-8705596 | MS.gene57646:CDS | 30.0% | |
| ! | AGAGTTTTCGTCGTAATATT+TGG | + | chr8.4:8705900-8705919 | None:intergenic | 30.0% |
| ! | ATTTAATGAAGGTGTGTTCA+AGG | - | chr8.4:8706261-8706280 | MS.gene57646:CDS | 30.0% |
| ! | CAATTTCTGGAAAAAAGGAT+AGG | + | chr8.4:8705817-8705836 | None:intergenic | 30.0% |
| ! | GGAAATTGAAATTTTGCTTC+AGG | - | chr8.4:8706078-8706097 | MS.gene57646:intron | 30.0% |
| ! | TTTAATGAAGGTGTGTTCAA+GGG | - | chr8.4:8706262-8706281 | MS.gene57646:CDS | 30.0% |
| !! | AACATGTTACTTACCCTTTT+GGG | + | chr8.4:8705245-8705264 | None:intergenic | 30.0% |
| !! | GAATTATTTTATGCGGTTCT+TGG | - | chr8.4:8705164-8705183 | MS.gene57646:CDS | 30.0% |
| !!! | CTGCTGTAATGTTTTGTTTA+AGG | + | chr8.4:8706294-8706313 | None:intergenic | 30.0% |
| AATTCAGACATTCTAGATCC+TGG | + | chr8.4:8706244-8706263 | None:intergenic | 35.0% | |
| ATACTGATGAGCTAACACTA+AGG | - | chr8.4:8706170-8706189 | MS.gene57646:intron | 35.0% | |
| ATTTCTTAACATGTGCCCTA+AGG | + | chr8.4:8705455-8705474 | None:intergenic | 35.0% | |
| GTTTCTAAACTTGTGCCTTA+AGG | - | chr8.4:8705576-8705595 | MS.gene57646:CDS | 35.0% | |
| TACTGATGAGCTAACACTAA+GGG | - | chr8.4:8706171-8706190 | MS.gene57646:intron | 35.0% | |
| TTCTAGATCCTGGAACAAAT+AGG | + | chr8.4:8706234-8706253 | None:intergenic | 35.0% | |
| TTTCTTAACATGTGCCCTAA+GGG | + | chr8.4:8705454-8705473 | None:intergenic | 35.0% | |
| ! | CTTCATATGACGAGTCTTTT+TGG | + | chr8.4:8705969-8705988 | None:intergenic | 35.0% |
| !! | GAACATGTTACTTACCCTTT+TGG | + | chr8.4:8705246-8705265 | None:intergenic | 35.0% |
| !! | TTATTTTATGCGGTTCTTGG+AGG | - | chr8.4:8705167-8705186 | MS.gene57646:CDS | 35.0% |
| AAAAAGGATAGGTCTAGTGG+TGG | + | chr8.4:8705806-8705825 | None:intergenic | 40.0% | |
| AATAGGTCTAACCAAGGATG+TGG | + | chr8.4:8706217-8706236 | None:intergenic | 40.0% | |
| AATGTTAACGTGTGCCCTTA+AGG | + | chr8.4:8705594-8705613 | None:intergenic | 40.0% | |
| ATAGGTCTAACCAAGGATGT+GGG | + | chr8.4:8706216-8706235 | None:intergenic | 40.0% | |
| CTCTCAAGATCATGTTGATC+AGG | - | chr8.4:8705916-8705935 | MS.gene57646:intron | 40.0% | |
| GGAAAAAAGGATAGGTCTAG+TGG | + | chr8.4:8705809-8705828 | None:intergenic | 40.0% | |
| GGAACAAATAGGTCTAACCA+AGG | + | chr8.4:8706223-8706242 | None:intergenic | 40.0% | |
| TATGCCATATTGGGTTACCA+AGG | + | chr8.4:8705858-8705877 | None:intergenic | 40.0% | |
| TGGTTAGACCTATTTGTTCC+AGG | - | chr8.4:8706223-8706242 | MS.gene57646:CDS | 40.0% | |
| ! | AACGACTTCTCTGCATTTTC+TGG | - | chr8.4:8705695-8705714 | MS.gene57646:CDS | 40.0% |
| ! | ACTTCCTTGGTAACCCAATA+TGG | - | chr8.4:8705851-8705870 | MS.gene57646:intron | 40.0% |
| ! | AGTGTTAACTTGAGCCCTTA+GGG | - | chr8.4:8705437-8705456 | MS.gene57646:intron | 40.0% |
| ! | ATGCGAGGTTGATCAATTTC+TGG | + | chr8.4:8705830-8705849 | None:intergenic | 40.0% |
| ! | CCAATTTGGGATTATAACCC+GGG | - | chr8.4:8705196-8705215 | MS.gene57646:CDS | 40.0% |
| ! | GCCAATTTGGGATTATAACC+CGG | - | chr8.4:8705195-8705214 | MS.gene57646:CDS | 40.0% |
| ! | TAGTGTTAACTTGAGCCCTT+AGG | - | chr8.4:8705436-8705455 | MS.gene57646:intron | 40.0% |
| CCCGGGTTATAATCCCAAAT+TGG | + | chr8.4:8705199-8705218 | None:intergenic | 45.0% | |
| GTACCCAATGAACCGAACAA+AGG | - | chr8.4:8706327-8706346 | MS.gene57646:CDS | 45.0% | |
| GTTACCAAGGAAGTTATGCG+AGG | + | chr8.4:8705845-8705864 | None:intergenic | 45.0% | |
| TCAACCTCGCATAACTTCCT+TGG | - | chr8.4:8705838-8705857 | MS.gene57646:intron | 45.0% | |
| TTGGAGGTCTAGGCCAATTT+GGG | - | chr8.4:8705183-8705202 | MS.gene57646:CDS | 45.0% | |
| ! | AGCTAACACTAAGGGCACAA+GGG | - | chr8.4:8706179-8706198 | MS.gene57646:intron | 45.0% |
| ! | TTTGGGATATCCCACATCCT+TGG | - | chr8.4:8706203-8706222 | MS.gene57646:intron | 45.0% |
| !! | TTACTTACCCTTTTGGGTGC+AGG | + | chr8.4:8705239-8705258 | None:intergenic | 45.0% |
| !!! | TTTAATTAGTAATTTAATTT+GGG | + | chr8.4:8705626-8705645 | None:intergenic | 5.0% |
| CTTGGAGGTCTAGGCCAATT+TGG | - | chr8.4:8705182-8705201 | MS.gene57646:CDS | 50.0% | |
| GTCCAAGAGCTATTCTTGCC+CGG | + | chr8.4:8705217-8705236 | None:intergenic | 50.0% | |
| TATGCGGTTCTTGGAGGTCT+AGG | - | chr8.4:8705173-8705192 | MS.gene57646:CDS | 50.0% | |
| TCCAAGAGCTATTCTTGCCC+GGG | + | chr8.4:8705216-8705235 | None:intergenic | 50.0% | |
| TCTTGGACCTGCACCCAAAA+GGG | - | chr8.4:8705229-8705248 | MS.gene57646:CDS | 50.0% | |
| ! | ACTAAGGGCACAAGGGCTTT+GGG | - | chr8.4:8706186-8706205 | MS.gene57646:intron | 50.0% |
| ! | GAGCTAACACTAAGGGCACA+AGG | - | chr8.4:8706178-8706197 | MS.gene57646:intron | 50.0% |
| ACCCGGGCAAGAATAGCTCT+TGG | - | chr8.4:8705212-8705231 | MS.gene57646:CDS | 55.0% | |
| CTCTTGGACCTGCACCCAAA+AGG | - | chr8.4:8705228-8705247 | MS.gene57646:CDS | 55.0% | |
| ! | CACTAAGGGCACAAGGGCTT+TGG | - | chr8.4:8706185-8706204 | MS.gene57646:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 8705123 | 8706347 | 8705123 | ID=MS.gene57646 |
| chr8.4 | mRNA | 8705123 | 8706347 | 8705123 | ID=MS.gene57646.t1;Parent=MS.gene57646 |
| chr8.4 | exon | 8706220 | 8706347 | 8706220 | ID=MS.gene57646.t1.exon1;Parent=MS.gene57646.t1 |
| chr8.4 | CDS | 8706220 | 8706347 | 8706220 | ID=cds.MS.gene57646.t1;Parent=MS.gene57646.t1 |
| chr8.4 | exon | 8705533 | 8705799 | 8705533 | ID=MS.gene57646.t1.exon2;Parent=MS.gene57646.t1 |
| chr8.4 | CDS | 8705533 | 8705799 | 8705533 | ID=cds.MS.gene57646.t1;Parent=MS.gene57646.t1 |
| chr8.4 | exon | 8705123 | 8705391 | 8705123 | ID=MS.gene57646.t1.exon3;Parent=MS.gene57646.t1 |
| chr8.4 | CDS | 8705123 | 8705391 | 8705123 | ID=cds.MS.gene57646.t1;Parent=MS.gene57646.t1 |
| Gene Sequence |
| Protein sequence |
