Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57652.t1 | XP_003610042.1 | 87.8 | 329 | 14 | 2 | 1 | 303 | 1 | 329 | 4.90E-148 | 533.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57652.t1 | Q9LU73 | 55.7 | 237 | 64 | 5 | 1 | 200 | 1 | 233 | 8.5e-48 | 192.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57652.t1 | G7JU94 | 87.8 | 329 | 14 | 2 | 1 | 303 | 1 | 329 | 3.6e-148 | 533.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051067 | MS.gene57652 | 0.822137 | 2.78E-53 | -1.69E-46 |
| MS.gene055537 | MS.gene57652 | 0.807209 | 5.65E-50 | -1.69E-46 |
| MS.gene057082 | MS.gene57652 | 0.802362 | 5.82E-49 | -1.69E-46 |
| MS.gene058823 | MS.gene57652 | 0.803412 | 3.53E-49 | -1.69E-46 |
| MS.gene059300 | MS.gene57652 | 0.808814 | 2.57E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGAAAACATTGCCTTTC+TGG | 0.197917 | 8.4:+7424724 | MS.gene57652:CDS |
| TTGCTGCTGCTGCTGTTCTA+TGG | 0.269718 | 8.4:-7423528 | None:intergenic |
| GCTTGACCCTTCATTCTTCC+AGG | 0.278647 | 8.4:+7423921 | MS.gene57652:CDS |
| TTTAAATGTTATAATAGTTC+TGG | 0.304204 | 8.4:+7423707 | MS.gene57652:CDS |
| ATTCTCTTGTGTTGGCTAGA+AGG | 0.358333 | 8.4:+7423233 | MS.gene57652:CDS |
| TTTGAAGGAACTTAGTCTTA+AGG | 0.360363 | 8.4:-7423305 | None:intergenic |
| TTTCAGACAGAACAATTTCA+TGG | 0.360555 | 8.4:+7423802 | MS.gene57652:CDS |
| AAAGCCCTTTAGCAGTAATA+AGG | 0.361117 | 8.4:+7424621 | MS.gene57652:CDS |
| TGGCACAGAAGAACAAAATA+AGG | 0.368675 | 8.4:+7424758 | MS.gene57652:CDS |
| TAAGACTAAGTTCCTTCAAA+AGG | 0.375696 | 8.4:+7423308 | MS.gene57652:CDS |
| CCTTTCTGGCTTCAGTCTCA+TGG | 0.390835 | 8.4:+7424738 | MS.gene57652:CDS |
| TTGTTTCCAGCATATGAGAT+AGG | 0.393515 | 8.4:-7424600 | None:intergenic |
| TTGATGATCCTAGTGTTAGT+AGG | 0.397262 | 8.4:+7423606 | MS.gene57652:CDS |
| GTGGATGTTGTGAAGTTTGA+TGG | 0.403077 | 8.4:-7423357 | None:intergenic |
| CTGTGATTCTCACTAGTTGA+AGG | 0.411339 | 8.4:-7423758 | None:intergenic |
| AGTAGGGTTATGAGAGAAGC+TGG | 0.424536 | 8.4:+7423623 | MS.gene57652:CDS |
| TGCACCTTATTACTGCTAAA+GGG | 0.429521 | 8.4:-7424625 | None:intergenic |
| CAATGCAGCAATGAGAGCAT+TGG | 0.451420 | 8.4:-7423474 | None:intergenic |
| TTTGAAGCATTCTCTTGTGT+TGG | 0.454108 | 8.4:+7423225 | MS.gene57652:CDS |
| AATATTACCTGGAAGAATGA+AGG | 0.464963 | 8.4:-7423928 | None:intergenic |
| ATGCACCTTATTACTGCTAA+AGG | 0.466662 | 8.4:-7424626 | None:intergenic |
| GCAGCAGCAGCAACAGCAAA+CGG | 0.470948 | 8.4:+7423538 | MS.gene57652:CDS |
| AAGACTAAGTTCCTTCAAAA+GGG | 0.480191 | 8.4:+7423309 | MS.gene57652:CDS |
| ATCAAAGGAGAAGGTGTTGT+TGG | 0.481111 | 8.4:-7423431 | None:intergenic |
| ACTGAGTTGACGAGTACATC+AGG | 0.485948 | 8.4:+7423890 | MS.gene57652:CDS |
| TGATGATCCTAGTGTTAGTA+GGG | 0.489344 | 8.4:+7423607 | MS.gene57652:CDS |
| AGATGCTCATCTTGGAATCA+AGG | 0.504959 | 8.4:-7424570 | None:intergenic |
| GTTCTGAGAGATGCTCATCT+TGG | 0.505466 | 8.4:-7424578 | None:intergenic |
| GAAGGCTGAGAGTGAATCAA+AGG | 0.518124 | 8.4:-7423446 | None:intergenic |
| TGTTGTGAAGTTTGATGGTG+TGG | 0.518229 | 8.4:-7423352 | None:intergenic |
| TCAGAACCTATCTCATATGC+TGG | 0.523908 | 8.4:+7424594 | MS.gene57652:CDS |
| CCATGAGACTGAAGCCAGAA+AGG | 0.527419 | 8.4:-7424738 | None:intergenic |
| ACGAGTACATCAGGTGGACT+TGG | 0.541210 | 8.4:+7423899 | MS.gene57652:CDS |
| ATATATCAAGTGATGCGCTC+AGG | 0.544488 | 8.4:+7423157 | None:intergenic |
| TGAAGCAGCCTCTGCTGTGA+GGG | 0.545563 | 8.4:-7423200 | None:intergenic |
| GCTCAATTTCTCAGACCTGC+AGG | 0.549147 | 8.4:+7424699 | MS.gene57652:intron |
| AAATGTTATAATAGTTCTGG+TGG | 0.549816 | 8.4:+7423710 | MS.gene57652:CDS |
| ATATTACCTGGAAGAATGAA+GGG | 0.561317 | 8.4:-7423927 | None:intergenic |
| TGTTCTGTCTGAAACTGCTA+AGG | 0.561994 | 8.4:-7423793 | None:intergenic |
| GCACAAGCTCATCAAAGAAG+AGG | 0.562580 | 8.4:+7423503 | MS.gene57652:CDS |
| TCACTAGTTGAAGGTGAACA+TGG | 0.565455 | 8.4:-7423749 | None:intergenic |
| TGAGAGTGAATCAAAGGAGA+AGG | 0.577096 | 8.4:-7423440 | None:intergenic |
| CTCATAACCCTACTAACACT+AGG | 0.579503 | 8.4:-7423614 | None:intergenic |
| AAAGTGATGGCTACATGTAG+AGG | 0.593224 | 8.4:-7423277 | None:intergenic |
| TCTTAAGGTAAGTAAAGTGA+TGG | 0.602666 | 8.4:-7423290 | None:intergenic |
| CTTGTGTTGGCTAGAAGGAG+AGG | 0.606431 | 8.4:+7423238 | MS.gene57652:CDS |
| CTGAAGCAGCCTCTGCTGTG+AGG | 0.607118 | 8.4:-7423201 | None:intergenic |
| ATGAGAGCATTGGAAAGTGA+AGG | 0.611410 | 8.4:-7423464 | None:intergenic |
| TAGCAGTAATAAGGTGCATG+AGG | 0.621940 | 8.4:+7424630 | MS.gene57652:CDS |
| TATCAAGTGATGCGCTCAGG+AGG | 0.632949 | 8.4:+7423160 | None:intergenic |
| TGTTATAATAGTTCTGGTGG+TGG | 0.635592 | 8.4:+7423713 | MS.gene57652:CDS |
| GCAGCAGACCCTCACAGCAG+AGG | 0.647393 | 8.4:+7423192 | MS.gene57652:CDS |
| TAGAAGGAGAGGTCATCCAC+AGG | 0.649362 | 8.4:+7423249 | MS.gene57652:CDS |
| ACATGTAGAGGAGTGACCTG+TGG | 0.684775 | 8.4:-7423265 | None:intergenic |
| GAGTTGACGAGTACATCAGG+TGG | 0.710146 | 8.4:+7423893 | MS.gene57652:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAATGTTATAATAGTTC+TGG | + | chr8.4:7423707-7423726 | MS.gene57652:CDS | 15.0% |
| !!! | TTCTTAATTTGTTTGATTTA+AGG | + | chr8.4:7424071-7424090 | MS.gene57652:intron | 15.0% |
| !! | AAGCAAAATAAAATATTACC+TGG | - | chr8.4:7423942-7423961 | None:intergenic | 20.0% |
| !! | CATTTAAAAACAGAATTAGA+AGG | - | chr8.4:7423695-7423714 | None:intergenic | 20.0% |
| !! | CCATAATTAAGCAAATAAAT+AGG | - | chr8.4:7424517-7424536 | None:intergenic | 20.0% |
| !! | CCTATTTATTTGCTTAATTA+TGG | + | chr8.4:7424514-7424533 | MS.gene57652:intron | 20.0% |
| !!! | TGTTTGATTTAAGGTATAAA+AGG | + | chr8.4:7424080-7424099 | MS.gene57652:intron | 20.0% |
| ! | AAAGACTACACTTTATCTAA+AGG | + | chr8.4:7424399-7424418 | MS.gene57652:intron | 25.0% |
| ! | ACACATGTTATGTTACTAAA+AGG | - | chr8.4:7424052-7424071 | None:intergenic | 25.0% |
| ! | ATATACACTTAGTACATGTT+TGG | + | chr8.4:7423971-7423990 | MS.gene57652:intron | 25.0% |
| ! | TTAAATGCAACCAAAAAGAA+AGG | + | chr8.4:7424118-7424137 | MS.gene57652:intron | 25.0% |
| !! | CTTATGTTTACCTTTCTTTT+TGG | - | chr8.4:7424131-7424150 | None:intergenic | 25.0% |
| !!! | AAATGTTATAATAGTTCTGG+TGG | + | chr8.4:7423710-7423729 | MS.gene57652:CDS | 25.0% |
| !!! | TCAGTCAAATTATTTTGAGT+TGG | + | chr8.4:7424368-7424387 | MS.gene57652:intron | 25.0% |
| !!! | TTGAGCTTCTTTTTCATAAT+TGG | - | chr8.4:7424685-7424704 | None:intergenic | 25.0% |
| AAGACTAAGTTCCTTCAAAA+GGG | + | chr8.4:7423309-7423328 | MS.gene57652:CDS | 30.0% | |
| AATATTACCTGGAAGAATGA+AGG | - | chr8.4:7423931-7423950 | None:intergenic | 30.0% | |
| ATATTACCTGGAAGAATGAA+GGG | - | chr8.4:7423930-7423949 | None:intergenic | 30.0% | |
| GTTTCAATGTAGCTCTTAAT+AGG | + | chr8.4:7423405-7423424 | MS.gene57652:intron | 30.0% | |
| TAAGACTAAGTTCCTTCAAA+AGG | + | chr8.4:7423308-7423327 | MS.gene57652:CDS | 30.0% | |
| TCTTAAGGTAAGTAAAGTGA+TGG | - | chr8.4:7423293-7423312 | None:intergenic | 30.0% | |
| TTTCAGACAGAACAATTTCA+TGG | + | chr8.4:7423802-7423821 | MS.gene57652:CDS | 30.0% | |
| !! | ACACATGTTAAAAGCATTGT+TGG | + | chr8.4:7424257-7424276 | MS.gene57652:intron | 30.0% |
| !! | ATGTTAAAAGCATTGTTGGT+TGG | + | chr8.4:7424261-7424280 | MS.gene57652:intron | 30.0% |
| !! | TTTGAAGGAACTTAGTCTTA+AGG | - | chr8.4:7423308-7423327 | None:intergenic | 30.0% |
| AAAGCCCTTTAGCAGTAATA+AGG | + | chr8.4:7424621-7424640 | MS.gene57652:CDS | 35.0% | |
| AGAAGAAAACATTGCCTTTC+TGG | + | chr8.4:7424724-7424743 | MS.gene57652:CDS | 35.0% | |
| ATGCACCTTATTACTGCTAA+AGG | - | chr8.4:7424629-7424648 | None:intergenic | 35.0% | |
| CTAAAAGGTTAGTGCATGTT+TGG | - | chr8.4:7424037-7424056 | None:intergenic | 35.0% | |
| TGATGATCCTAGTGTTAGTA+GGG | + | chr8.4:7423607-7423626 | MS.gene57652:CDS | 35.0% | |
| TGCACCTTATTACTGCTAAA+GGG | - | chr8.4:7424628-7424647 | None:intergenic | 35.0% | |
| TTGACTGATTAACGTGTAAG+AGG | - | chr8.4:7424356-7424375 | None:intergenic | 35.0% | |
| TTGTTTCCAGCATATGAGAT+AGG | - | chr8.4:7424603-7424622 | None:intergenic | 35.0% | |
| ! | AATGTGTTGAGGAGTATGTA+CGG | + | chr8.4:7424216-7424235 | MS.gene57652:intron | 35.0% |
| ! | TTGATGATCCTAGTGTTAGT+AGG | + | chr8.4:7423606-7423625 | MS.gene57652:CDS | 35.0% |
| ! | TTTGAAGCATTCTCTTGTGT+TGG | + | chr8.4:7423225-7423244 | MS.gene57652:CDS | 35.0% |
| !! | AAAGTGTTGTCTAGTTCTGA+TGG | + | chr8.4:7424163-7424182 | MS.gene57652:intron | 35.0% |
| !! | ATTTGTTGTGCAATGTGTTG+AGG | + | chr8.4:7424205-7424224 | MS.gene57652:intron | 35.0% |
| !! | TGTTATAATAGTTCTGGTGG+TGG | + | chr8.4:7423713-7423732 | MS.gene57652:CDS | 35.0% |
| AAAGTGATGGCTACATGTAG+AGG | - | chr8.4:7423280-7423299 | None:intergenic | 40.0% | |
| AGATGCTCATCTTGGAATCA+AGG | - | chr8.4:7424573-7424592 | None:intergenic | 40.0% | |
| CTCATAACCCTACTAACACT+AGG | - | chr8.4:7423617-7423636 | None:intergenic | 40.0% | |
| CTGTGATTCTCACTAGTTGA+AGG | - | chr8.4:7423761-7423780 | None:intergenic | 40.0% | |
| GTGGATGTTGTGAAGTTTGA+TGG | - | chr8.4:7423360-7423379 | None:intergenic | 40.0% | |
| TAGCAGTAATAAGGTGCATG+AGG | + | chr8.4:7424630-7424649 | MS.gene57652:CDS | 40.0% | |
| TATGTACGGTTACTGAGCTA+TGG | + | chr8.4:7424230-7424249 | MS.gene57652:intron | 40.0% | |
| TCACTAGTTGAAGGTGAACA+TGG | - | chr8.4:7423752-7423771 | None:intergenic | 40.0% | |
| TCAGAACCTATCTCATATGC+TGG | + | chr8.4:7424594-7424613 | MS.gene57652:CDS | 40.0% | |
| TGAGAGTGAATCAAAGGAGA+AGG | - | chr8.4:7423443-7423462 | None:intergenic | 40.0% | |
| ! | AATGTTTTCTTCTGTCCTGC+AGG | - | chr8.4:7424717-7424736 | None:intergenic | 40.0% |
| ! | CTCGTCAACTCAGTTCTTTT+TGG | - | chr8.4:7423884-7423903 | None:intergenic | 40.0% |
| ! | CTTAAGACAAGCCCTTTTGA+AGG | - | chr8.4:7423323-7423342 | None:intergenic | 40.0% |
| ! | GTCAACTCAGTTCTTTTTGG+TGG | - | chr8.4:7423881-7423900 | None:intergenic | 40.0% |
| ! | TGTTCTGTCTGAAACTGCTA+AGG | - | chr8.4:7423796-7423815 | None:intergenic | 40.0% |
| !! | AGCATTGTTGGTTGGTAGTA+AGG | + | chr8.4:7424269-7424288 | MS.gene57652:intron | 40.0% |
| !! | ATCAAAGGAGAAGGTGTTGT+TGG | - | chr8.4:7423434-7423453 | None:intergenic | 40.0% |
| !! | ATGAGAGCATTGGAAAGTGA+AGG | - | chr8.4:7423467-7423486 | None:intergenic | 40.0% |
| !! | ATTCTCTTGTGTTGGCTAGA+AGG | + | chr8.4:7423233-7423252 | MS.gene57652:CDS | 40.0% |
| !! | TGTTGTGAAGTTTGATGGTG+TGG | - | chr8.4:7423355-7423374 | None:intergenic | 40.0% |
| AGTAGGGTTATGAGAGAAGC+TGG | + | chr8.4:7423623-7423642 | MS.gene57652:CDS | 45.0% | |
| GAAGGCTGAGAGTGAATCAA+AGG | - | chr8.4:7423449-7423468 | None:intergenic | 45.0% | |
| GCACAAGCTCATCAAAGAAG+AGG | + | chr8.4:7423503-7423522 | MS.gene57652:CDS | 45.0% | |
| ! | ACTGAGTTGACGAGTACATC+AGG | + | chr8.4:7423890-7423909 | MS.gene57652:CDS | 45.0% |
| ! | CAATGCAGCAATGAGAGCAT+TGG | - | chr8.4:7423477-7423496 | None:intergenic | 45.0% |
| !! | CAAGAGCTCTAGATTGACAG+TGG | - | chr8.4:7423379-7423398 | None:intergenic | 45.0% |
| !! | GTTCTGAGAGATGCTCATCT+TGG | - | chr8.4:7424581-7424600 | None:intergenic | 45.0% |
| ACATGTAGAGGAGTGACCTG+TGG | - | chr8.4:7423268-7423287 | None:intergenic | 50.0% | |
| CCATGAGACTGAAGCCAGAA+AGG | - | chr8.4:7424741-7424760 | None:intergenic | 50.0% | |
| GAGTTGACGAGTACATCAGG+TGG | + | chr8.4:7423893-7423912 | MS.gene57652:CDS | 50.0% | |
| GCTCAATTTCTCAGACCTGC+AGG | + | chr8.4:7424699-7424718 | MS.gene57652:intron | 50.0% | |
| GCTTGACCCTTCATTCTTCC+AGG | + | chr8.4:7423921-7423940 | MS.gene57652:CDS | 50.0% | |
| TAGAAGGAGAGGTCATCCAC+AGG | + | chr8.4:7423249-7423268 | MS.gene57652:CDS | 50.0% | |
| TTGCTGCTGCTGCTGTTCTA+TGG | - | chr8.4:7423531-7423550 | None:intergenic | 50.0% | |
| ! | ACGAGTACATCAGGTGGACT+TGG | + | chr8.4:7423899-7423918 | MS.gene57652:CDS | 50.0% |
| !! | CCTTTCTGGCTTCAGTCTCA+TGG | + | chr8.4:7424738-7424757 | MS.gene57652:CDS | 50.0% |
| !! | CTTGTGTTGGCTAGAAGGAG+AGG | + | chr8.4:7423238-7423257 | MS.gene57652:CDS | 50.0% |
| GCAGCAGCAGCAACAGCAAA+CGG | + | chr8.4:7423538-7423557 | MS.gene57652:CDS | 55.0% | |
| TGAAGCAGCCTCTGCTGTGA+GGG | - | chr8.4:7423203-7423222 | None:intergenic | 55.0% | |
| CTGAAGCAGCCTCTGCTGTG+AGG | - | chr8.4:7423204-7423223 | None:intergenic | 60.0% | |
| GCAGCAGACCCTCACAGCAG+AGG | + | chr8.4:7423192-7423211 | MS.gene57652:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 7423167 | 7424779 | 7423167 | ID=MS.gene57652 |
| chr8.4 | mRNA | 7423167 | 7424779 | 7423167 | ID=MS.gene57652.t1;Parent=MS.gene57652 |
| chr8.4 | exon | 7423167 | 7423380 | 7423167 | ID=MS.gene57652.t1.exon1;Parent=MS.gene57652.t1 |
| chr8.4 | CDS | 7423167 | 7423380 | 7423167 | ID=cds.MS.gene57652.t1;Parent=MS.gene57652.t1 |
| chr8.4 | exon | 7423427 | 7423942 | 7423427 | ID=MS.gene57652.t1.exon2;Parent=MS.gene57652.t1 |
| chr8.4 | CDS | 7423427 | 7423942 | 7423427 | ID=cds.MS.gene57652.t1;Parent=MS.gene57652.t1 |
| chr8.4 | exon | 7424567 | 7424667 | 7424567 | ID=MS.gene57652.t1.exon3;Parent=MS.gene57652.t1 |
| chr8.4 | CDS | 7424567 | 7424667 | 7424567 | ID=cds.MS.gene57652.t1;Parent=MS.gene57652.t1 |
| chr8.4 | exon | 7424700 | 7424779 | 7424700 | ID=MS.gene57652.t1.exon4;Parent=MS.gene57652.t1 |
| chr8.4 | CDS | 7424700 | 7424779 | 7424700 | ID=cds.MS.gene57652.t1;Parent=MS.gene57652.t1 |
| Gene Sequence |
| Protein sequence |
