Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57973.t1 | XP_003609498.2 | 96 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 1.30E-69 | 272.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57973.t1 | G7JGN5 | 96.0 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 9.5e-70 | 272.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053104 | MS.gene57973 | 0.802514 | 5.41E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene57973 | 0.802752 | 4.83E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene57973 | 0.807761 | 4.32E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene57973 | 0.804948 | 1.69E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57973.t1 | MTR_4g116360 | 96.000 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 3.92e-95 | 271 |
| MS.gene57973.t1 | MTR_2g450420 | 46.809 | 141 | 63 | 5 | 18 | 150 | 17 | 153 | 1.01e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCTCACACCTCAGTAAA+TGG | 0.325930 | 8.1:-10611142 | MS.gene57973:CDS |
| TAGACTCAATTAATTGTGTA+TGG | 0.388334 | 8.1:+10610985 | None:intergenic |
| CTGCAGGAAATATAAAGAAT+AGG | 0.441652 | 8.1:-10611331 | MS.gene57973:intron |
| CTAGAAATGTGAATGTGAAT+GGG | 0.472633 | 8.1:-10611114 | MS.gene57973:CDS |
| GTGTGTGCAGGGATACGTTT+GGG | 0.472885 | 8.1:-10612051 | MS.gene57973:intron |
| GTTATGTCTACAAGGTCCTC+AGG | 0.481183 | 8.1:+10611043 | None:intergenic |
| ACTAGAAATGTGAATGTGAA+TGG | 0.484726 | 8.1:-10611115 | MS.gene57973:CDS |
| GCAGGGATACGTTTGGGAAA+AGG | 0.491954 | 8.1:-10612045 | MS.gene57973:intron |
| TGTGTGTGCAGGGATACGTT+TGG | 0.497727 | 8.1:-10612052 | MS.gene57973:intron |
| TACAACTAAATCCTTGTCAA+AGG | 0.499214 | 8.1:-10611285 | MS.gene57973:intron |
| CCATCTCAGTTATGTCTACA+AGG | 0.517334 | 8.1:+10611035 | None:intergenic |
| CCTTGTAGACATAACTGAGA+TGG | 0.558484 | 8.1:-10611035 | MS.gene57973:CDS |
| TTTAGTTGTAGAAATTGAAG+TGG | 0.566395 | 8.1:+10611298 | None:intergenic |
| TGTGAAGAACACTGAGGATG+TGG | 0.567568 | 8.1:-10611161 | MS.gene57973:CDS |
| AGAGAGACTGCTACACAAGA+AGG | 0.574354 | 8.1:+10612183 | None:intergenic |
| AGACTCAATTAATTGTGTAT+GGG | 0.578163 | 8.1:+10610986 | None:intergenic |
| AAGAAGTAACACTGATTCTG+AGG | 0.585024 | 8.1:-10611858 | MS.gene57973:CDS |
| AGATGGATTATTCACCAGCT+AGG | 0.588549 | 8.1:-10611018 | MS.gene57973:CDS |
| TCTAGTATTGCCATTTACTG+AGG | 0.596383 | 8.1:+10611132 | None:intergenic |
| TTATAAACCAACCTTTGACA+AGG | 0.606206 | 8.1:+10611274 | None:intergenic |
| TTATGTCTACAAGGTCCTCA+GGG | 0.619907 | 8.1:+10611044 | None:intergenic |
| TAGAAATGTGAATGTGAATG+GGG | 0.652177 | 8.1:-10611113 | MS.gene57973:CDS |
| GAATGTGAATGGGGAAGCAA+AGG | 0.660105 | 8.1:-10611104 | MS.gene57973:CDS |
| TACTTCTAATTATCTCCCTG+AGG | 0.676211 | 8.1:-10611059 | MS.gene57973:CDS |
| ACAGAATGTGAAGAACACTG+AGG | 0.882326 | 8.1:-10611167 | MS.gene57973:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAGATGTAAAAAAAA+AGG | + | chr8.1:10611971-10611990 | None:intergenic | 10.0% |
| !! | ATAAATTATATTATAAACCT+TGG | - | chr8.1:10611940-10611959 | MS.gene57973:intron | 10.0% |
| !! | ATCATGAAAGAAATTTAATT+AGG | - | chr8.1:10611759-10611778 | MS.gene57973:intron | 15.0% |
| !! | TAATTAAATTTCTTTCATGA+TGG | + | chr8.1:10611760-10611779 | None:intergenic | 15.0% |
| !! | TAAAAAAAAAGGTAATTCCA+AGG | + | chr8.1:10611960-10611979 | None:intergenic | 20.0% |
| !! | TCAAATCTACAATTGTAAAA+TGG | + | chr8.1:10611585-10611604 | None:intergenic | 20.0% |
| !! | TGGAAAATTATTATCTAAAG+TGG | - | chr8.1:10611550-10611569 | MS.gene57973:intron | 20.0% |
| !!! | TTTTAAAAAAATTAGGGTAG+TGG | - | chr8.1:10611530-10611549 | MS.gene57973:intron | 20.0% |
| ! | AAAAAAGGAAGAAAAACTTG+TGG | + | chr8.1:10611508-10611527 | None:intergenic | 25.0% |
| ! | ATATGTCCTTGTAATTTCAA+AGG | + | chr8.1:10611615-10611634 | None:intergenic | 25.0% |
| ! | TTTAGTTGTAGAAATTGAAG+TGG | + | chr8.1:10611888-10611907 | None:intergenic | 25.0% |
| !! | ATTTACCTTGAGTTTTTGTA+AGG | + | chr8.1:10611044-10611063 | None:intergenic | 25.0% |
| !! | TTGCATAGTCATAAGTTTTT+GGG | + | chr8.1:10611665-10611684 | None:intergenic | 25.0% |
| !! | TTTGCATAGTCATAAGTTTT+TGG | + | chr8.1:10611666-10611685 | None:intergenic | 25.0% |
| !!! | AAATGTTTTTATTTTGCTGC+AGG | - | chr8.1:10611836-10611855 | MS.gene57973:CDS | 25.0% |
| ACTAGAAATGTGAATGTGAA+TGG | - | chr8.1:10612068-10612087 | MS.gene57973:intron | 30.0% | |
| CAGATTCATGAAGATTACTA+TGG | - | chr8.1:10611440-10611459 | MS.gene57973:intron | 30.0% | |
| CTAGAAATGTGAATGTGAAT+GGG | - | chr8.1:10612069-10612088 | MS.gene57973:intron | 30.0% | |
| CTGCAGGAAATATAAAGAAT+AGG | - | chr8.1:10611852-10611871 | MS.gene57973:CDS | 30.0% | |
| GGAAGATTAGAAATGTAACA+AGG | + | chr8.1:10611106-10611125 | None:intergenic | 30.0% | |
| TACAACTAAATCCTTGTCAA+AGG | - | chr8.1:10611898-10611917 | MS.gene57973:intron | 30.0% | |
| TAGAAATGTGAATGTGAATG+GGG | - | chr8.1:10612070-10612089 | MS.gene57973:intron | 30.0% | |
| TAGTAATCTTCATGAATCTG+TGG | + | chr8.1:10611440-10611459 | None:intergenic | 30.0% | |
| TATGACTATGCAAAAATGCA+AGG | - | chr8.1:10611672-10611691 | MS.gene57973:intron | 30.0% | |
| TGTGGACTTGAAAAAACTAA+TGG | + | chr8.1:10611490-10611509 | None:intergenic | 30.0% | |
| TTATAAACCAACCTTTGACA+AGG | + | chr8.1:10611912-10611931 | None:intergenic | 30.0% | |
| ! | TGCATGCTTTCACATTTTTA+TGG | - | chr8.1:10611242-10611261 | MS.gene57973:intron | 30.0% |
| !!! | AGTGTTACTTCTTTTCAACA+AGG | + | chr8.1:10611318-10611337 | None:intergenic | 30.0% |
| AATGACCAACAAAAATGCAC+AGG | - | chr8.1:10611362-10611381 | MS.gene57973:intron | 35.0% | |
| ACTAAATCCTTGTCAAAGGT+TGG | - | chr8.1:10611902-10611921 | MS.gene57973:intron | 35.0% | |
| CTTCTCCTTACAAAAACTCA+AGG | - | chr8.1:10611036-10611055 | MS.gene57973:CDS | 35.0% | |
| GACAAAATTTGTCAGCTTAG+TGG | + | chr8.1:10611643-10611662 | None:intergenic | 35.0% | |
| GAGCAACAAAAACAACATGT+AGG | - | chr8.1:10611174-10611193 | MS.gene57973:intron | 35.0% | |
| TACTTCTAATTATCTCCCTG+AGG | - | chr8.1:10612124-10612143 | MS.gene57973:intron | 35.0% | |
| TATTCTCTTCTCTTGGTTTC+TGG | - | chr8.1:10611698-10611717 | MS.gene57973:intron | 35.0% | |
| ! | ATTATCTAAAGTGGCGCTAA+TGG | - | chr8.1:10611559-10611578 | MS.gene57973:intron | 35.0% |
| ! | TAACTGGTTGTGTGATTTTC+TGG | + | chr8.1:10611417-10611436 | None:intergenic | 35.0% |
| ! | TCTAGTATTGCCATTTACTG+AGG | + | chr8.1:10612054-10612073 | None:intergenic | 35.0% |
| !! | AAGAAGTAACACTGATTCTG+AGG | - | chr8.1:10611325-10611344 | MS.gene57973:CDS | 35.0% |
| !! | AAGGCTTTATTCTCTTCTCT+TGG | - | chr8.1:10611691-10611710 | MS.gene57973:intron | 35.0% |
| !! | CTTCGTCCTTTGAAATTACA+AGG | - | chr8.1:10611606-10611625 | MS.gene57973:intron | 35.0% |
| !! | CTTTAGAAGCTGTTTTCTTC+AGG | + | chr8.1:10611391-10611410 | None:intergenic | 35.0% |
| ACAGAATGTGAAGAACACTG+AGG | - | chr8.1:10612016-10612035 | MS.gene57973:CDS | 40.0% | |
| CCATCTCAGTTATGTCTACA+AGG | + | chr8.1:10612151-10612170 | None:intergenic | 40.0% | |
| CCTTGTAGACATAACTGAGA+TGG | - | chr8.1:10612148-10612167 | MS.gene57973:CDS | 40.0% | |
| CTTCATGAATCTGTGGTAAC+TGG | + | chr8.1:10611433-10611452 | None:intergenic | 40.0% | |
| TATCCCTGCACACACAAAAA+AGG | + | chr8.1:10611127-10611146 | None:intergenic | 40.0% | |
| TTATGTCTACAAGGTCCTCA+GGG | + | chr8.1:10612142-10612161 | None:intergenic | 40.0% | |
| !! | AAGTTCACTTTTCTGACTGC+AGG | - | chr8.1:10611277-10611296 | MS.gene57973:intron | 40.0% |
| !! | AGATGGATTATTCACCAGCT+AGG | - | chr8.1:10612165-10612184 | MS.gene57973:CDS | 40.0% |
| !!! | CTTCCTTTTTTGTGTGTGCA+GGG | - | chr8.1:10611121-10611140 | MS.gene57973:CDS | 40.0% |
| !!! | TCTTCCTTTTTTGTGTGTGC+AGG | - | chr8.1:10611120-10611139 | MS.gene57973:CDS | 40.0% |
| AGAGAGACTGCTACACAAGA+AGG | + | chr8.1:10611003-10611022 | None:intergenic | 45.0% | |
| GAATGTGAATGGGGAAGCAA+AGG | - | chr8.1:10612079-10612098 | MS.gene57973:intron | 45.0% | |
| GACCTAGACGACATAGATCT+AGG | - | chr8.1:10611462-10611481 | MS.gene57973:intron | 45.0% | |
| GTTATGTCTACAAGGTCCTC+AGG | + | chr8.1:10612143-10612162 | None:intergenic | 45.0% | |
| TGCCTAGATCTATGTCGTCT+AGG | + | chr8.1:10611467-10611486 | None:intergenic | 45.0% | |
| TGTGAAGAACACTGAGGATG+TGG | - | chr8.1:10612022-10612041 | MS.gene57973:CDS | 45.0% | |
| ! | ACTTTTCTGACTGCAGGATG+AGG | - | chr8.1:10611283-10611302 | MS.gene57973:intron | 45.0% |
| ! | GGCTACCTGTGCATTTTTGT+TGG | + | chr8.1:10611370-10611389 | None:intergenic | 45.0% |
| !! | ATGGGAGACTTTTTCCTAGC+TGG | + | chr8.1:10612182-10612201 | None:intergenic | 45.0% |
| !!! | AATTTTTTTAAAAGAAAAAA+AGG | + | chr8.1:10611523-10611542 | None:intergenic | 5.0% |
| !!! | TTTTTCTTTTAAAAAAATTA+GGG | - | chr8.1:10611524-10611543 | MS.gene57973:intron | 5.0% |
| !!! | TTTTTTCTTTTAAAAAAATT+AGG | - | chr8.1:10611523-10611542 | MS.gene57973:intron | 5.0% |
| GCAGGGATACGTTTGGGAAA+AGG | - | chr8.1:10611138-10611157 | MS.gene57973:CDS | 50.0% | |
| GTGGCTCACACCTCAGTAAA+TGG | - | chr8.1:10612041-10612060 | MS.gene57973:CDS | 50.0% | |
| GTGTGTGCAGGGATACGTTT+GGG | - | chr8.1:10611132-10611151 | MS.gene57973:CDS | 50.0% | |
| TGTGTGTGCAGGGATACGTT+TGG | - | chr8.1:10611131-10611150 | MS.gene57973:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 10610994 | 10612211 | 10610994 | ID=MS.gene57973 |
| chr8.1 | mRNA | 10610994 | 10612211 | 10610994 | ID=MS.gene57973.t1;Parent=MS.gene57973 |
| chr8.1 | exon | 10612148 | 10612211 | 10612148 | ID=MS.gene57973.t1.exon1;Parent=MS.gene57973.t1 |
| chr8.1 | CDS | 10612148 | 10612211 | 10612148 | ID=cds.MS.gene57973.t1;Parent=MS.gene57973.t1 |
| chr8.1 | exon | 10612014 | 10612063 | 10612014 | ID=MS.gene57973.t1.exon2;Parent=MS.gene57973.t1 |
| chr8.1 | CDS | 10612014 | 10612063 | 10612014 | ID=cds.MS.gene57973.t1;Parent=MS.gene57973.t1 |
| chr8.1 | exon | 10611822 | 10611906 | 10611822 | ID=MS.gene57973.t1.exon3;Parent=MS.gene57973.t1 |
| chr8.1 | CDS | 10611822 | 10611906 | 10611822 | ID=cds.MS.gene57973.t1;Parent=MS.gene57973.t1 |
| chr8.1 | exon | 10611286 | 10611347 | 10611286 | ID=MS.gene57973.t1.exon4;Parent=MS.gene57973.t1 |
| chr8.1 | CDS | 10611286 | 10611347 | 10611286 | ID=cds.MS.gene57973.t1;Parent=MS.gene57973.t1 |
| chr8.1 | exon | 10610994 | 10611185 | 10610994 | ID=MS.gene57973.t1.exon5;Parent=MS.gene57973.t1 |
| chr8.1 | CDS | 10610994 | 10611185 | 10610994 | ID=cds.MS.gene57973.t1;Parent=MS.gene57973.t1 |
| Gene Sequence |
| Protein sequence |
