Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58023.t1 | XP_003609432.1 | 98.7 | 159 | 2 | 0 | 8 | 166 | 90 | 248 | 2.40E-72 | 281.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58023.t1 | Q9ASQ5 | 42.9 | 163 | 84 | 2 | 12 | 166 | 96 | 257 | 2.7e-16 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58023.t1 | G7JFF3 | 98.7 | 159 | 2 | 0 | 8 | 166 | 90 | 248 | 1.7e-72 | 281.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053278 | MS.gene58023 | 0.842034 | 3.31E-58 | -1.69E-46 |
| MS.gene060336 | MS.gene58023 | 0.836991 | 6.76E-57 | -1.69E-46 |
| MS.gene060337 | MS.gene58023 | 0.843012 | 1.82E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58023.t1 | MTR_4g115630 | 98.742 | 159 | 2 | 0 | 8 | 166 | 90 | 248 | 4.66e-110 | 322 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58023.t1 | AT2G11520 | 47.500 | 160 | 75 | 3 | 15 | 166 | 99 | 257 | 4.13e-33 | 122 |
| MS.gene58023.t1 | AT5G58940 | 43.529 | 85 | 47 | 1 | 82 | 166 | 96 | 179 | 9.37e-12 | 62.4 |
| MS.gene58023.t1 | AT5G58940 | 43.529 | 85 | 47 | 1 | 82 | 166 | 96 | 179 | 9.37e-12 | 62.4 |
| MS.gene58023.t1 | AT5G58940 | 43.529 | 85 | 47 | 1 | 82 | 166 | 96 | 179 | 9.37e-12 | 62.4 |
| MS.gene58023.t1 | AT5G58940 | 43.529 | 85 | 47 | 1 | 82 | 166 | 96 | 179 | 9.37e-12 | 62.4 |
Find 38 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATCAACTCACTTGAATT+TGG | 0.113511 | 8.3:+10933031 | None:intergenic |
| CAAATAGGAGAAGGAGGTTT+TGG | 0.222709 | 8.3:-10932388 | MS.gene58023:CDS |
| TTTGCTCTTGCAGTGGAATT+AGG | 0.233044 | 8.3:-10932577 | MS.gene58023:intron |
| GGCAACTTCTCATGCTGTTC+TGG | 0.280389 | 8.3:-10933062 | MS.gene58023:CDS |
| GTTGTTTGTCCTTGTTTCTA+TGG | 0.288044 | 8.3:-10933097 | MS.gene58023:CDS |
| GACTGAAGTCTGCTGAGTTT+TGG | 0.319257 | 8.3:+10932469 | None:intergenic |
| TGGCTGTAAAACGTGCAAAA+AGG | 0.327174 | 8.3:-10932327 | MS.gene58023:CDS |
| AGTTTCCTTCCTGCCTTTGA+AGG | 0.372344 | 8.3:+10933229 | None:intergenic |
| ACATCAACTCACTTGAATTT+GGG | 0.375068 | 8.3:+10933032 | None:intergenic |
| TTCTCATGCTGTTCTGGAAA+AGG | 0.431491 | 8.3:-10933056 | MS.gene58023:CDS |
| GTTAATTTGCCTTCAAAGGC+AGG | 0.446182 | 8.3:-10933238 | MS.gene58023:CDS |
| ATTACATCTCAACCTGAGTC+AGG | 0.448193 | 8.3:-10932446 | MS.gene58023:CDS |
| GGAGACATTGAGAATCTTGA+CGG | 0.458728 | 8.3:+10932490 | None:intergenic |
| TTGTTTGTCCTTGTTTCTAT+GGG | 0.461186 | 8.3:-10933096 | MS.gene58023:CDS |
| GATCCCGGCTAGTCCACTTC+GGG | 0.470734 | 8.3:-10932524 | MS.gene58023:CDS |
| GGCACCCGAAGTGGACTAGC+CGG | 0.472365 | 8.3:+10932520 | None:intergenic |
| TTCAGTTAATTTGCCTTCAA+AGG | 0.486885 | 8.3:-10933242 | MS.gene58023:CDS |
| TTTAGACGATGGCCTGGTTG+TGG | 0.491375 | 8.3:-10932347 | MS.gene58023:CDS |
| TTTCTATGGGAAGAGAAGAA+AGG | 0.495384 | 8.3:-10933083 | MS.gene58023:CDS |
| GGCTCATTTAGACGATGGCC+TGG | 0.511850 | 8.3:-10932353 | MS.gene58023:CDS |
| CTTCTCTTCCCATAGAAACA+AGG | 0.521374 | 8.3:+10933088 | None:intergenic |
| GCACCCGAAGTGGACTAGCC+GGG | 0.531645 | 8.3:+10932521 | None:intergenic |
| ATCAGAAACTGATGGTTCAA+AGG | 0.546450 | 8.3:+10932549 | None:intergenic |
| CAAGTGTCAAGCAGCCAAGT+TGG | 0.547377 | 8.3:-10933157 | MS.gene58023:CDS |
| AACAATAATTCGACGTCACA+AGG | 0.551644 | 8.3:-10933190 | MS.gene58023:CDS |
| AGATCCCGGCTAGTCCACTT+CGG | 0.553615 | 8.3:-10932525 | MS.gene58023:CDS |
| GGACTAGGTGGCACCCGAAG+TGG | 0.567503 | 8.3:+10932511 | None:intergenic |
| ATCAGTTTCTGATAAGATCC+CGG | 0.569707 | 8.3:-10932539 | MS.gene58023:CDS |
| ATTGAGAATCTTGACGGACT+AGG | 0.584446 | 8.3:+10932496 | None:intergenic |
| ATTTGCCTTCAAAGGCAGGA+AGG | 0.590231 | 8.3:-10933234 | MS.gene58023:CDS |
| GAAACATTACAAATAGGAGA+AGG | 0.594509 | 8.3:-10932397 | MS.gene58023:CDS |
| TAGCTTTAGAAACCTGACTC+AGG | 0.608297 | 8.3:+10932434 | None:intergenic |
| ACATTACAAATAGGAGAAGG+AGG | 0.614749 | 8.3:-10932394 | MS.gene58023:CDS |
| AGAAACTGATGGTTCAAAGG+AGG | 0.644221 | 8.3:+10932552 | None:intergenic |
| TACAAGGCTCATTTAGACGA+TGG | 0.650447 | 8.3:-10932358 | MS.gene58023:CDS |
| GGGATCTTATCAGAAACTGA+TGG | 0.663836 | 8.3:+10932541 | None:intergenic |
| GAGAATCTTGACGGACTAGG+TGG | 0.679579 | 8.3:+10932499 | None:intergenic |
| ATAGAAACAAGGACAAACAA+CGG | 0.698270 | 8.3:+10933099 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATAAATGAAAACTGTT+TGG | + | chr8.3:10932656-10932675 | None:intergenic | 15.0% |
| !! | ACAATGGAAACTAAAAATTA+AGG | + | chr8.3:10932942-10932961 | None:intergenic | 20.0% |
| !! | CAATGGAAACTAAAAATTAA+GGG | + | chr8.3:10932941-10932960 | None:intergenic | 20.0% |
| !! | TTCCCTATAATTTATGAAAA+CGG | + | chr8.3:10932843-10932862 | None:intergenic | 20.0% |
| !!! | AATCCGTTTTCATAAATTAT+AGG | - | chr8.3:10932837-10932856 | MS.gene58023:intron | 20.0% |
| !!! | ATCCGTTTTCATAAATTATA+GGG | - | chr8.3:10932838-10932857 | MS.gene58023:intron | 20.0% |
| !!! | GTAATGTTTCTGAAAAATTA+CGG | + | chr8.3:10933179-10933198 | None:intergenic | 20.0% |
| !!! | TAATGTTTCTGAAAAATTAC+GGG | + | chr8.3:10933178-10933197 | None:intergenic | 20.0% |
| !!! | TTTTCAGAAACATTACAAAT+AGG | - | chr8.3:10933183-10933202 | MS.gene58023:CDS | 20.0% |
| ! | AGATTGTACTTTATTACCTT+AGG | - | chr8.3:10932617-10932636 | MS.gene58023:intron | 25.0% |
| ! | GATTGTACTTTATTACCTTA+GGG | - | chr8.3:10932618-10932637 | MS.gene58023:intron | 25.0% |
| AAACTGTTTGGTAAGTAAAC+AGG | + | chr8.3:10932644-10932663 | None:intergenic | 30.0% | |
| AACATCAACTCACTTGAATT+TGG | + | chr8.3:10932558-10932577 | None:intergenic | 30.0% | |
| ACATCAACTCACTTGAATTT+GGG | + | chr8.3:10932557-10932576 | None:intergenic | 30.0% | |
| ATAGAAACAAGGACAAACAA+CGG | + | chr8.3:10932490-10932509 | None:intergenic | 30.0% | |
| CTACTAGTTATCAAACTATG+AGG | + | chr8.3:10932599-10932618 | None:intergenic | 30.0% | |
| GAAACATTACAAATAGGAGA+AGG | - | chr8.3:10933189-10933208 | MS.gene58023:CDS | 30.0% | |
| GTTATCAAACTATGAGGTAA+AGG | + | chr8.3:10932593-10932612 | None:intergenic | 30.0% | |
| !! | TTCAGTTAATTTGCCTTCAA+AGG | - | chr8.3:10932344-10932363 | MS.gene58023:CDS | 30.0% |
| !! | TTGTTTGTCCTTGTTTCTAT+GGG | - | chr8.3:10932490-10932509 | MS.gene58023:CDS | 30.0% |
| AACAATAATTCGACGTCACA+AGG | - | chr8.3:10932396-10932415 | MS.gene58023:CDS | 35.0% | |
| ACATTACAAATAGGAGAAGG+AGG | - | chr8.3:10933192-10933211 | MS.gene58023:CDS | 35.0% | |
| ACTAGTCATTCAAAGGTAAG+AGG | + | chr8.3:10932979-10932998 | None:intergenic | 35.0% | |
| ATCAGAAACTGATGGTTCAA+AGG | + | chr8.3:10933040-10933059 | None:intergenic | 35.0% | |
| ATCAGTTTCTGATAAGATCC+CGG | - | chr8.3:10933047-10933066 | MS.gene58023:CDS | 35.0% | |
| GTTCATCACTAGTCATTCAA+AGG | + | chr8.3:10932986-10933005 | None:intergenic | 35.0% | |
| TTGAAGAGAAGATGAAAAGC+AGG | - | chr8.3:10932895-10932914 | MS.gene58023:intron | 35.0% | |
| TTTCTATGGGAAGAGAAGAA+AGG | - | chr8.3:10932503-10932522 | MS.gene58023:CDS | 35.0% | |
| ! | GGAAGTGCAATCTATAACAA+TGG | + | chr8.3:10932958-10932977 | None:intergenic | 35.0% |
| !! | GTTGTTTGTCCTTGTTTCTA+TGG | - | chr8.3:10932489-10932508 | MS.gene58023:CDS | 35.0% |
| !! | TTCATTTTAAGTCGCTGACA+AGG | - | chr8.3:10932682-10932701 | MS.gene58023:intron | 35.0% |
| AGAAACTGATGGTTCAAAGG+AGG | + | chr8.3:10933037-10933056 | None:intergenic | 40.0% | |
| AGACAGACAGACAGATAGTT+AGG | - | chr8.3:10932749-10932768 | MS.gene58023:intron | 40.0% | |
| ATTACATCTCAACCTGAGTC+AGG | - | chr8.3:10933140-10933159 | MS.gene58023:CDS | 40.0% | |
| CTTCTCTTCCCATAGAAACA+AGG | + | chr8.3:10932501-10932520 | None:intergenic | 40.0% | |
| GACAGACAGACAGATAGTTA+GGG | - | chr8.3:10932750-10932769 | MS.gene58023:intron | 40.0% | |
| GGAGACATTGAGAATCTTGA+CGG | + | chr8.3:10933099-10933118 | None:intergenic | 40.0% | |
| GGGATCTTATCAGAAACTGA+TGG | + | chr8.3:10933048-10933067 | None:intergenic | 40.0% | |
| TACAAGGCTCATTTAGACGA+TGG | - | chr8.3:10933228-10933247 | MS.gene58023:CDS | 40.0% | |
| TGGCTGTAAAACGTGCAAAA+AGG | - | chr8.3:10933259-10933278 | MS.gene58023:CDS | 40.0% | |
| TTTGCTCTTGCAGTGGAATT+AGG | - | chr8.3:10933009-10933028 | MS.gene58023:intron | 40.0% | |
| ! | CAAATAGGAGAAGGAGGTTT+TGG | - | chr8.3:10933198-10933217 | MS.gene58023:CDS | 40.0% |
| ! | GAACAATTTTGCTCTTGCAG+TGG | - | chr8.3:10933002-10933021 | MS.gene58023:intron | 40.0% |
| !! | AGCCAAGTTGGAATTTTTGC+GGG | - | chr8.3:10932441-10932460 | MS.gene58023:CDS | 40.0% |
| !! | ATTGAGAATCTTGACGGACT+AGG | + | chr8.3:10933093-10933112 | None:intergenic | 40.0% |
| !! | CAGCCAAGTTGGAATTTTTG+CGG | - | chr8.3:10932440-10932459 | MS.gene58023:CDS | 40.0% |
| !! | GTTAATTTGCCTTCAAAGGC+AGG | - | chr8.3:10932348-10932367 | MS.gene58023:CDS | 40.0% |
| !! | TAGCTTTAGAAACCTGACTC+AGG | + | chr8.3:10933155-10933174 | None:intergenic | 40.0% |
| !! | TTCTCATGCTGTTCTGGAAA+AGG | - | chr8.3:10932530-10932549 | MS.gene58023:CDS | 40.0% |
| !!! | AGGTTTTGGAACTGTCTACA+AGG | - | chr8.3:10933212-10933231 | MS.gene58023:CDS | 40.0% |
| AGTTTCCTTCCTGCCTTTGA+AGG | + | chr8.3:10932360-10932379 | None:intergenic | 45.0% | |
| ATTTGCCTTCAAAGGCAGGA+AGG | - | chr8.3:10932352-10932371 | MS.gene58023:CDS | 45.0% | |
| CACCCGCAAAAATTCCAACT+TGG | + | chr8.3:10932446-10932465 | None:intergenic | 45.0% | |
| TGGTAAGTAAACAGGCCCTA+AGG | + | chr8.3:10932636-10932655 | None:intergenic | 45.0% | |
| ! | GACTGAAGTCTGCTGAGTTT+TGG | + | chr8.3:10933120-10933139 | None:intergenic | 45.0% |
| !! | CAAGTTGGAATTTTTGCGGG+TGG | - | chr8.3:10932444-10932463 | MS.gene58023:CDS | 45.0% |
| CAAGTGTCAAGCAGCCAAGT+TGG | - | chr8.3:10932429-10932448 | MS.gene58023:CDS | 50.0% | |
| GGCAACTTCTCATGCTGTTC+TGG | - | chr8.3:10932524-10932543 | MS.gene58023:CDS | 50.0% | |
| TTTAGACGATGGCCTGGTTG+TGG | - | chr8.3:10933239-10933258 | MS.gene58023:CDS | 50.0% | |
| ! | CACGTTTTACAGCCACAACC+AGG | + | chr8.3:10933254-10933273 | None:intergenic | 50.0% |
| !! | GAGAATCTTGACGGACTAGG+TGG | + | chr8.3:10933090-10933109 | None:intergenic | 50.0% |
| !! | TTTTGCGGGTGGAGCTTTGT+TGG | - | chr8.3:10932455-10932474 | MS.gene58023:CDS | 50.0% |
| GGCTCATTTAGACGATGGCC+TGG | - | chr8.3:10933233-10933252 | MS.gene58023:CDS | 55.0% | |
| ! | AGATCCCGGCTAGTCCACTT+CGG | - | chr8.3:10933061-10933080 | MS.gene58023:CDS | 55.0% |
| ! | GATCCCGGCTAGTCCACTTC+GGG | - | chr8.3:10933062-10933081 | MS.gene58023:CDS | 60.0% |
| GGACTAGGTGGCACCCGAAG+TGG | + | chr8.3:10933078-10933097 | None:intergenic | 65.0% | |
| !! | GCACCCGAAGTGGACTAGCC+GGG | + | chr8.3:10933068-10933087 | None:intergenic | 65.0% |
| !! | GGCACCCGAAGTGGACTAGC+CGG | + | chr8.3:10933069-10933088 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 10932327 | 10933281 | 10932327 | ID=MS.gene58023 |
| chr8.3 | mRNA | 10932327 | 10933281 | 10932327 | ID=MS.gene58023.t1;Parent=MS.gene58023 |
| chr8.3 | exon | 10933044 | 10933281 | 10933044 | ID=MS.gene58023.t1.exon1;Parent=MS.gene58023.t1 |
| chr8.3 | CDS | 10933044 | 10933281 | 10933044 | ID=cds.MS.gene58023.t1;Parent=MS.gene58023.t1 |
| chr8.3 | exon | 10932327 | 10932586 | 10932327 | ID=MS.gene58023.t1.exon2;Parent=MS.gene58023.t1 |
| chr8.3 | CDS | 10932327 | 10932586 | 10932327 | ID=cds.MS.gene58023.t1;Parent=MS.gene58023.t1 |
| Gene Sequence |
| Protein sequence |
