Alfalfa Gene Editing Database
Nr:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene053278.t1 | XP_003604146.2 | 98.4 | 516 | 8 | 0 | 1 | 516 | 87 | 602 | 1.30E-273 | 951.8 |
Swissprot:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene053278.t1 | Q9LTB0 | 74.9 | 517 | 124 | 4 | 1 | 516 | 81 | 592 | 2.0e-206 | 719.9 |
Trembl:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene053278.t1 | G7JVK6 | 98.4 | 516 | 8 | 0 | 1 | 516 | 87 | 602 | 9.3e-274 | 951.8 |
TFs/TRs:
Gene ID | Type | Classification |
---|
Protein Kinases:
Gene ID | Type | Classification |
---|
Network:
Co-expression Network:
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MS.gene053278 | MS.gene055719 | 0.811483 | 6.84E-51 | -1.69E-46 |
MS.gene053278 | MS.gene06297 | 0.80758 | 4.72E-50 | -1.69E-46 |
MS.gene053278 | MS.gene06713 | 0.807045 | 6.12E-50 | -1.69E-46 |
MS.gene053278 | MS.gene070003 | 0.804782 | 1.83E-49 | -1.69E-46 |
MS.gene053278 | MS.gene09078 | 0.801997 | 6.91E-49 | -1.69E-46 |
MS.gene053278 | MS.gene33980 | 0.816616 | 5.05E-52 | -1.69E-46 |
MS.gene053278 | MS.gene34509 | 0.834136 | 3.57E-56 | -1.69E-46 |
MS.gene053278 | MS.gene40337 | 0.819557 | 1.09E-52 | -1.69E-46 |
MS.gene053278 | MS.gene41628 | 0.801051 | 1.08E-48 | -1.69E-46 |
MS.gene053278 | MS.gene43154 | 0.81062 | 1.05E-50 | -1.69E-46 |
MS.gene053278 | MS.gene47799 | 0.801196 | 1.01E-48 | -1.69E-46 |
MS.gene053278 | MS.gene50521 | 0.810627 | 1.05E-50 | -1.69E-46 |
MS.gene053278 | MS.gene57987 | 0.805535 | 1.27E-49 | -1.69E-46 |
MS.gene053278 | MS.gene58023 | 0.842034 | 3.31E-58 | -1.69E-46 |
MS.gene053278 | MS.gene67063 | 0.801511 | 8.70E-49 | -1.69E-46 |
MS.gene053278 | MS.gene76513 | 0.804711 | 1.89E-49 | -1.69E-46 |
MS.gene053278 | MS.gene80717 | 0.803937 | 2.75E-49 | -1.69E-46 |
MS.gene053278 | MS.gene82544 | 0.80268 | 5.00E-49 | -1.69E-46 |
MS.gene053278 | MS.gene85128 | 0.801507 | 8.72E-49 | -1.69E-46 |
MS.gene053278 | MS.gene97437 | 0.801216 | 1.00E-48 | -1.69E-46 |
MS.gene053278 | MS.gene97861 | 0.818048 | 2.40E-52 | -1.69E-46 |
MS.gene053278 | MS.gene98153 | 0.824818 | 6.57E-54 | -1.69E-46 |
PPI:
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 0 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!! | GACAAAATAAAAAGATGAAA+AGG | - | 32807:1049891-1049910 | None:intergenic | 20.0% |
!!! | ATTTTCAATTTACTTTAAGC+TGG | + | 32807:1050066-1050085 | MS.gene053278:intron | 20.0% |
!!! | GGTAACTGTTTTTTTTTTAA+TGG | - | 32807:1049724-1049743 | None:intergenic | 20.0% |
!!! | TTTTCAATTTACTTTAAGCT+GGG | + | 32807:1050067-1050086 | MS.gene053278:intron | 20.0% |
! | AAAAAACCACAAACTACATA+AGG | - | 32807:1050046-1050065 | None:intergenic | 25.0% |
! | AAAAACCACAAACTACATAA+GGG | - | 32807:1050045-1050064 | None:intergenic | 25.0% |
! | AAAATTCACATTGCAAATCA+AGG | - | 32807:1049745-1049764 | None:intergenic | 25.0% |
! | ATCATAGACTAATAGACTAA+AGG | - | 32807:1049845-1049864 | None:intergenic | 25.0% |
! | TAATTGTGTGAAAAAACAGA+TGG | + | 32807:1049789-1049808 | MS.gene053278:intron | 25.0% |
! | TATGACATAGAATGATGTTT+GGG | + | 32807:1049626-1049645 | MS.gene053278:intron | 25.0% |
! | TTATGACATAGAATGATGTT+TGG | + | 32807:1049625-1049644 | MS.gene053278:intron | 25.0% |
!! | CTAAAAAGTTTCAAAAGTAC+AGG | - | 32807:1049390-1049409 | None:intergenic | 25.0% |
!!! | CAGCTGCATTATTTTAATAT+TGG | + | 32807:1049988-1050007 | MS.gene053278:intron | 25.0% |
!!! | GGTATTTTTTATGCTTATAC+AGG | + | 32807:1050088-1050107 | MS.gene053278:intron | 25.0% |
ACATAAAGAAATATCAGACC+TGG | + | 32807:1048726-1048745 | MS.gene053278:CDS | 30.0% | |
AGCTGACATGTATATAAGTA+TGG | + | 32807:1050556-1050575 | MS.gene053278:CDS | 30.0% | |
ATAGACTAATAGACTAAAGG+TGG | - | 32807:1049842-1049861 | None:intergenic | 30.0% | |
GACATGTATATAAGTATGGA+AGG | + | 32807:1050560-1050579 | MS.gene053278:CDS | 30.0% | |
TACAGAAAAACCATCAGAAT+CGG | - | 32807:1048686-1048705 | None:intergenic | 30.0% | |
TATAAGTATGGAAGGAAATG+CGG | + | 32807:1050568-1050587 | MS.gene053278:CDS | 30.0% | |
TGGAAACAAAATTGTACGAA+TGG | + | 32807:1050259-1050278 | MS.gene053278:CDS | 30.0% | |
TTTCAGCCCTTAAAAAACTT+GGG | + | 32807:1048998-1049017 | MS.gene053278:CDS | 30.0% | |
! | TCTGATGGTTTTTCTGTAAA+TGG | + | 32807:1048688-1048707 | MS.gene053278:CDS | 30.0% |
! | TTTTTCTGTAAATGGTACAC+TGG | + | 32807:1048696-1048715 | MS.gene053278:CDS | 30.0% |
!! | TGAGTAGTTGTTCTAAAACT+AGG | + | 32807:1049684-1049703 | MS.gene053278:intron | 30.0% |
AAAATCGTCATCCTGAACAA+CGG | + | 32807:1050426-1050445 | MS.gene053278:CDS | 35.0% | |
AAATATCAGACCTGGACAAA+TGG | + | 32807:1048734-1048753 | MS.gene053278:CDS | 35.0% | |
AACCATCAAATTGAAGCGAT+GGG | - | 32807:1049571-1049590 | None:intergenic | 35.0% | |
AAGAGAACATCCAAAAGATC+AGG | - | 32807:1048772-1048791 | None:intergenic | 35.0% | |
AAGGAGATATCCGATTTAGA+AGG | + | 32807:1048568-1048587 | MS.gene053278:CDS | 35.0% | |
AGAATTCTATTACCGAACGT+AGG | + | 32807:1048902-1048921 | MS.gene053278:CDS | 35.0% | |
AGTATAACATGCAAAGTCTC+CGG | + | 32807:1049073-1049092 | MS.gene053278:CDS | 35.0% | |
ATTCTTCTGAACTTGTGATG+TGG | + | 32807:1049211-1049230 | MS.gene053278:CDS | 35.0% | |
CATATCCTCCTAATGCTAAT+CGG | + | 32807:1049499-1049518 | MS.gene053278:CDS | 35.0% | |
CTTACAGGAATCTTGGTTTA+GGG | - | 32807:1050375-1050394 | None:intergenic | 35.0% | |
GAGTGTGTTCAAATAGCATT+GGG | + | 32807:1049324-1049343 | MS.gene053278:CDS | 35.0% | |
GTTTCAGCCCTTAAAAAACT+TGG | + | 32807:1048997-1049016 | MS.gene053278:CDS | 35.0% | |
TATCATCCTTACAGGAATCT+TGG | - | 32807:1050382-1050401 | None:intergenic | 35.0% | |
TCTAATGCTTGTTCAACACT+AGG | - | 32807:1049414-1049433 | None:intergenic | 35.0% | |
TGAAAGCTCACCTTCTAAAT+CGG | - | 32807:1048581-1048600 | None:intergenic | 35.0% | |
TTATCGATATCCGATTCTGA+TGG | + | 32807:1048673-1048692 | MS.gene053278:CDS | 35.0% | |
TTCAGCCCTTAAAAAACTTG+GGG | + | 32807:1048999-1049018 | MS.gene053278:CDS | 35.0% | |
TTCTAAATCGGATATCTCCT+TGG | - | 32807:1048569-1048588 | None:intergenic | 35.0% | |
TTCTTCTGAACTTGTGATGT+GGG | + | 32807:1049212-1049231 | MS.gene053278:CDS | 35.0% | |
TTGAAATGCTGTTGTACTAC+CGG | - | 32807:1049529-1049548 | None:intergenic | 35.0% | |
! | AGCTAATTTTTGCCTACGTT+CGG | - | 32807:1048917-1048936 | None:intergenic | 35.0% |
! | AGTTGAGTTTCCTGATCTTT+TGG | + | 32807:1048759-1048778 | MS.gene053278:CDS | 35.0% |
! | TGATCTTTTGGATGTTCTCT+TGG | + | 32807:1048771-1048790 | MS.gene053278:CDS | 35.0% |
! | TTTGAGCCTGATTTTTACAG+AGG | + | 32807:1050514-1050533 | MS.gene053278:CDS | 35.0% |
!! | AAAAGCACTACCAGAATCAA+TGG | + | 32807:1050211-1050230 | MS.gene053278:CDS | 35.0% |
!! | AGTGACTCATTGGCTATTTT+TGG | + | 32807:1049174-1049193 | MS.gene053278:CDS | 35.0% |
!!! | ACCATCCCCAAGTTTTTTAA+GGG | - | 32807:1049007-1049026 | None:intergenic | 35.0% |
!!! | TCAGGTGAGACTTTTAGTTT+TGG | + | 32807:1049590-1049609 | MS.gene053278:intron | 35.0% |
AAATCAGGCTCAAAGCATGT+TGG | - | 32807:1050508-1050527 | None:intergenic | 40.0% | |
AAATCGTCATCCTGAACAAC+GGG | + | 32807:1050427-1050446 | MS.gene053278:CDS | 40.0% | |
AACTACTCACATTGTCCCAA+CGG | - | 32807:1049673-1049692 | None:intergenic | 40.0% | |
AATAGTGATATAGCAGCCGT+TGG | + | 32807:1049654-1049673 | MS.gene053278:intron | 40.0% | |
ACAAGTTCAGAAGAATACGC+TGG | - | 32807:1049207-1049226 | None:intergenic | 40.0% | |
ACTCAACTAACCATTTGTCC+AGG | - | 32807:1048747-1048766 | None:intergenic | 40.0% | |
ACTCAAGCAACCTTGATACA+TGG | + | 32807:1048622-1048641 | MS.gene053278:CDS | 40.0% | |
AGCAACCTTGATACATGGTT+TGG | + | 32807:1048627-1048646 | MS.gene053278:CDS | 40.0% | |
AGTATATGCTCCTCCATATG+AGG | - | 32807:1049109-1049128 | None:intergenic | 40.0% | |
AGTGGATAGTAGGTTTCTGA+TGG | - | 32807:1048605-1048624 | None:intergenic | 40.0% | |
ATAGTGATATAGCAGCCGTT+GGG | + | 32807:1049655-1049674 | MS.gene053278:intron | 40.0% | |
ATGTATCAAGGTTGCTTGAG+TGG | - | 32807:1048623-1048642 | None:intergenic | 40.0% | |
CAAACTACATAAGGGAAACG+TGG | - | 32807:1050037-1050056 | None:intergenic | 40.0% | |
CACCATCCTCTGTAAAAATC+AGG | - | 32807:1050523-1050542 | None:intergenic | 40.0% | |
CATTTGCTCTTCTGGTGAAA+AGG | + | 32807:1049253-1049272 | MS.gene053278:CDS | 40.0% | |
CCTAAACCAAGATTCCTGTA+AGG | + | 32807:1050373-1050392 | MS.gene053278:CDS | 40.0% | |
CCTTACAGGAATCTTGGTTT+AGG | - | 32807:1050376-1050395 | None:intergenic | 40.0% | |
CGTTTCCCTTATGTAGTTTG+TGG | + | 32807:1050037-1050056 | MS.gene053278:intron | 40.0% | |
GAAATGCGGATGAAGTTGAA+TGG | + | 32807:1050582-1050601 | MS.gene053278:CDS | 40.0% | |
GAACCATCAAATTGAAGCGA+TGG | - | 32807:1049572-1049591 | None:intergenic | 40.0% | |
GCAGAGACAGAAGTAAAGAT+GGG | - | 32807:1048882-1048901 | None:intergenic | 40.0% | |
GCTTATACAGGACTTCTTTG+AGG | + | 32807:1050100-1050119 | MS.gene053278:intron | 40.0% | |
GGAGTGTGTTCAAATAGCAT+TGG | + | 32807:1049323-1049342 | MS.gene053278:CDS | 40.0% | |
TAGTTCATCAGCCAGTAATG+AGG | - | 32807:1050324-1050343 | None:intergenic | 40.0% | |
TCTACGGTTATCATCCTTAC+AGG | - | 32807:1050390-1050409 | None:intergenic | 40.0% | |
TGAATCGATGTGAACTCCAT+CGG | - | 32807:1048653-1048672 | None:intergenic | 40.0% | |
TGCAGAGACAGAAGTAAAGA+TGG | - | 32807:1048883-1048902 | None:intergenic | 40.0% | |
TGCTCATTATTAGAAGCTCG+TGG | + | 32807:1049351-1049370 | MS.gene053278:CDS | 40.0% | |
! | AGCCTGATTTTTACAGAGGA+TGG | + | 32807:1050518-1050537 | MS.gene053278:CDS | 40.0% |
! | ATGACGATTTTGCCTTTCTG+TGG | - | 32807:1050417-1050436 | None:intergenic | 40.0% |
! | TCGCTTCAATTTGATGGTTC+AGG | + | 32807:1049572-1049591 | MS.gene053278:CDS | 40.0% |
!! | AACAGAAGCATTTGCTCTTC+TGG | + | 32807:1049245-1049264 | MS.gene053278:CDS | 40.0% |
!! | GAAGAAAGCTTTGAGGATTC+TGG | + | 32807:1050239-1050258 | MS.gene053278:CDS | 40.0% |
!! | TCTTCTTCGTCCATTGATTC+TGG | - | 32807:1050224-1050243 | None:intergenic | 40.0% |
!!! | CTCATTGGCTATTTTTGGTG+AGG | + | 32807:1049179-1049198 | MS.gene053278:CDS | 40.0% |
!!! | GACCATCCCCAAGTTTTTTA+AGG | - | 32807:1049008-1049027 | None:intergenic | 40.0% |
AAGGCGAGTAGAAGAAGCTT+TGG | + | 32807:1048798-1048817 | MS.gene053278:CDS | 45.0% | |
AATCTGCTGGGCTTCAGTTT+CGG | - | 32807:1050285-1050304 | None:intergenic | 45.0% | |
ACAAGCTGCAAGTGACTCAT+TGG | + | 32807:1049164-1049183 | MS.gene053278:CDS | 45.0% | |
ACAGGAATCTTGGTTTAGGG+AGG | - | 32807:1050372-1050391 | None:intergenic | 45.0% | |
ACCGTAGAAGAACCACAGAA+AGG | + | 32807:1050402-1050421 | MS.gene053278:CDS | 45.0% | |
ACCTCTGCTTAGTATGCAGT+TGG | + | 32807:1050130-1050149 | MS.gene053278:CDS | 45.0% | |
ACGTTCTCCTTCATCAAGAG+CGG | - | 32807:1048824-1048843 | None:intergenic | 45.0% | |
AGGTTGCTTGAGTGGATAGT+AGG | - | 32807:1048615-1048634 | None:intergenic | 45.0% | |
ATCGGCCAAACCATGTATCA+AGG | - | 32807:1048635-1048654 | None:intergenic | 45.0% | |
ATGCACGAAGTTCAGATCCA+CGG | - | 32807:1048975-1048994 | None:intergenic | 45.0% | |
CCTCTGCTTAGTATGCAGTT+GGG | + | 32807:1050131-1050150 | MS.gene053278:CDS | 45.0% | |
GCCCTTAAAAAACTTGGGGA+TGG | + | 32807:1049003-1049022 | MS.gene053278:CDS | 45.0% | |
GCCTTTCTGTGGTTCTTCTA+CGG | - | 32807:1050406-1050425 | None:intergenic | 45.0% | |
GCTAGCTAATGCCTCATTAC+TGG | + | 32807:1050310-1050329 | MS.gene053278:CDS | 45.0% | |
GGACGAAGAAGAAAGCTTTG+AGG | + | 32807:1050232-1050251 | MS.gene053278:CDS | 45.0% | |
TATGGCTGCTGCTGATGATT+GGG | + | 32807:1049470-1049489 | MS.gene053278:CDS | 45.0% | |
TGCCCATCGCTTCAATTTGA+TGG | + | 32807:1049566-1049585 | MS.gene053278:CDS | 45.0% | |
TGGATGTTCTCTTGGCTGAA+AGG | + | 32807:1048779-1048798 | MS.gene053278:CDS | 45.0% | |
! | CAGGACTTCTTTGAGGATGT+AGG | + | 32807:1050107-1050126 | MS.gene053278:intron | 45.0% |
! | CCTCCATATGAGGTGTTTGA+CGG | - | 32807:1049099-1049118 | None:intergenic | 45.0% |
! | TCTGGTGAAAAGGCATGCAT+TGG | + | 32807:1049263-1049282 | MS.gene053278:CDS | 45.0% |
! | TTGATACATGGTTTGGCCGA+TGG | + | 32807:1048634-1048653 | MS.gene053278:CDS | 45.0% |
!! | ACAAGAGAGTACTGCTGCTA+TGG | + | 32807:1049452-1049471 | MS.gene053278:CDS | 45.0% |
AGCTAGCAGTGCAATCTGCT+GGG | - | 32807:1050297-1050316 | None:intergenic | 50.0% | |
AGGAGGTTTAAGAGCTGCTG+CGG | + | 32807:1049302-1049321 | MS.gene053278:CDS | 50.0% | |
AGTAGCAAGCTGTGAGCATG+CGG | - | 32807:1049030-1049049 | None:intergenic | 50.0% | |
AGTTCAGATCCACGGGTAGA+AGG | - | 32807:1048967-1048986 | None:intergenic | 50.0% | |
ATCCTGAACAACGGGAATGG+AGG | + | 32807:1050435-1050454 | MS.gene053278:CDS | 50.0% | |
CCCAACTGCATACTAAGCAG+AGG | - | 32807:1050134-1050153 | None:intergenic | 50.0% | |
CCGTCAAACACCTCATATGG+AGG | + | 32807:1049096-1049115 | MS.gene053278:CDS | 50.0% | |
CCTAATGCTAATCGGCAGAC+CGG | + | 32807:1049507-1049526 | MS.gene053278:CDS | 50.0% | |
CTATGGCTGCTGCTGATGAT+TGG | + | 32807:1049469-1049488 | MS.gene053278:CDS | 50.0% | |
CTGCTTAGTATGCAGTTGGG+AGG | + | 32807:1050134-1050153 | MS.gene053278:CDS | 50.0% | |
GTCATCCTGAACAACGGGAA+TGG | + | 32807:1050432-1050451 | MS.gene053278:CDS | 50.0% | |
GTTTAGGGAGGAGAGCTTCA+TGG | - | 32807:1050360-1050379 | None:intergenic | 50.0% | |
TAGCTAGCAGTGCAATCTGC+TGG | - | 32807:1050298-1050317 | None:intergenic | 50.0% | |
TGCACGAAGTTCAGATCCAC+GGG | - | 32807:1048974-1048993 | None:intergenic | 50.0% | |
! | CATATGAGGTGTTTGACGGC+CGG | - | 32807:1049095-1049114 | None:intergenic | 50.0% |
! | GTCTGCCGATTAGCATTAGG+AGG | - | 32807:1049507-1049526 | None:intergenic | 50.0% |
! | TACTGCAGCTCTAGCACAAC+TGG | + | 32807:1049125-1049144 | MS.gene053278:CDS | 50.0% |
CGGCCGTCAAACACCTCATA+TGG | + | 32807:1049093-1049112 | MS.gene053278:CDS | 55.0% | |
CTCCTCCATTCCCGTTGTTC+AGG | - | 32807:1050440-1050459 | None:intergenic | 55.0% | |
GAATGGAGGAGACGACTCGT+GGG | + | 32807:1050449-1050468 | MS.gene053278:CDS | 55.0% | |
GACTCGTGGGTTCAGTTGAC+AGG | + | 32807:1050462-1050481 | MS.gene053278:CDS | 55.0% | |
GCTTTGGCCGCTCTTGATGA+AGG | + | 32807:1048814-1048833 | MS.gene053278:CDS | 55.0% | |
TTGGCTTCATCAGCAGCTGC+AGG | + | 32807:1049282-1049301 | MS.gene053278:CDS | 55.0% | |
! | CCGGTCTGCCGATTAGCATT+AGG | - | 32807:1049510-1049529 | None:intergenic | 55.0% |
!! | TGGGAGGACAAGCTCTGGAA+GGG | + | 32807:1050150-1050169 | MS.gene053278:CDS | 55.0% |
!! | TTGGGAGGACAAGCTCTGGA+AGG | + | 32807:1050149-1050168 | MS.gene053278:CDS | 55.0% |
GCAGTTGGGAGGACAAGCTC+TGG | + | 32807:1050145-1050164 | MS.gene053278:CDS | 60.0% | |
GCTTCATCAGCAGCTGCAGG+AGG | + | 32807:1049285-1049304 | MS.gene053278:CDS | 60.0% | |
GCTTGTCAGCCTTCTACCCG+TGG | + | 32807:1048955-1048974 | MS.gene053278:CDS | 60.0% | |
GGAATGGAGGAGACGACTCG+TGG | + | 32807:1050448-1050467 | MS.gene053278:CDS | 60.0% | |
GAGAGCTTCATGGCTGCGCG+TGG | - | 32807:1050350-1050369 | None:intergenic | 65.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
32807 | gene | 1048561 | 1050625 | 1048561 | ID=MS.gene053278 |
32807 | mRNA | 1048561 | 1050625 | 1048561 | ID=MS.gene053278.t1;Parent=MS.gene053278 |
32807 | exon | 1048561 | 1049593 | 1048561 | ID=MS.gene053278.t1.exon1;Parent=MS.gene053278.t1 |
32807 | CDS | 1048561 | 1049593 | 1048561 | ID=cds.MS.gene053278.t1;Parent=MS.gene053278.t1 |
32807 | exon | 1050110 | 1050625 | 1050110 | ID=MS.gene053278.t1.exon2;Parent=MS.gene053278.t1 |
32807 | CDS | 1050110 | 1050625 | 1050110 | ID=cds.MS.gene053278.t1;Parent=MS.gene053278.t1 |
Gene Sequence |
Protein sequence |