AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58161

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58161.t1 MTR_8g092060 98.326 239 4 0 31 269 791 1029 1.30e-165 485
MS.gene58161.t1 MTR_2g028030 57.983 238 81 4 31 266 785 1005 5.38e-78 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58161.t1 AT1G10490 53.165 237 105 3 31 266 796 1027 2.56e-68 228
MS.gene58161.t1 AT1G10490 53.165 237 105 3 31 266 796 1027 2.56e-68 228
MS.gene58161.t1 AT3G57940 46.330 218 109 2 31 248 787 996 1.35e-49 175
MS.gene58161.t1 AT3G57940 46.330 218 109 2 31 248 792 1001 1.45e-49 175

Find 45 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTGTCTCTATTTATAA+AGG 0.271442 4.2:+10121226 MS.gene58161:CDS
CAGCCATAAATCAAGTAAAA+AGG 0.320830 4.2:+10121944 MS.gene58161:CDS
GCATACCGCTGGAGCAATTC+AGG 0.326482 4.2:-10121762 None:intergenic
TTGTCAGCAGTTGTCTTGAT+AGG 0.327701 4.2:-10120014 None:intergenic
TTCTGTTTAACAGGGACAAA+TGG 0.330082 4.2:+10121187 MS.gene58161:intron
GACAATGCCTTGCAAAATAC+TGG 0.353277 4.2:+10121810 MS.gene58161:CDS
TCTACATGGTCTTGCATTCA+AGG 0.399145 4.2:+10121271 MS.gene58161:CDS
CAATCTTGCTGACTTTCATC+TGG 0.401834 4.2:+10120103 MS.gene58161:CDS
TCTGTTTAACAGGGACAAAT+GGG 0.420355 4.2:+10121188 MS.gene58161:intron
CTGCACAGGTCTGCAGAATC+AGG 0.422621 4.2:+10120769 MS.gene58161:CDS
TGAGCATGTGATAATGTAAC+TGG 0.428130 4.2:-10120731 None:intergenic
TATATAAGTATATGAGGATA+AGG 0.438678 4.2:-10119160 None:intergenic
CAGCGGTATGCCTTTGAAGA+TGG 0.471481 4.2:+10121774 MS.gene58161:CDS
TATATGTATATAAGTATATG+AGG 0.491166 4.2:-10119166 None:intergenic
GGCTATCCTTCTTTAAAACA+AGG 0.510932 4.2:+10119111 MS.gene58161:CDS
GCTTGTGTGTTACTCTGCAC+AGG 0.520610 4.2:+10120755 MS.gene58161:CDS
TTGTCAGTATAAAATCGAAC+CGG 0.524708 4.2:+10121856 MS.gene58161:CDS
TACATCTTTCAGCCGTGGCA+TGG 0.532959 4.2:-10121306 None:intergenic
TCAGGACATTTCGCACATTG+AGG 0.533303 4.2:+10120787 MS.gene58161:CDS
AGGGACAAATGGGGTTGGAA+AGG 0.535630 4.2:+10121198 MS.gene58161:intron
GGAACCTCTTAGTGTTTCTG+TGG 0.536334 4.2:+10121547 MS.gene58161:CDS
TGGATGAAGACCTCAACGAT+GGG 0.551646 4.2:+10121567 MS.gene58161:CDS
CATCTTCAAAGGCATACCGC+TGG 0.566759 4.2:-10121773 None:intergenic
GCTAGATTCTCCATCTTCAA+AGG 0.571573 4.2:-10121784 None:intergenic
TACTGTATAGGACGATATGA+AGG 0.574051 4.2:+10121716 MS.gene58161:intron
TTAACAGGGACAAATGGGGT+TGG 0.574911 4.2:+10121193 MS.gene58161:intron
AAGTACAGGTGCGAAAGTGC+AGG 0.580964 4.2:-10120695 None:intergenic
TATACTGACAAGACCACCTG+TGG 0.585384 4.2:-10121844 None:intergenic
TTAAAGTCTGAAAACGAGCA+TGG 0.587411 4.2:+10121882 MS.gene58161:CDS
CGATGGGGCTAAACAAGTTG+AGG 0.590660 4.2:+10121583 MS.gene58161:CDS
TTAAAACAAGGTTTAGTGTG+CGG 0.597179 4.2:+10119123 MS.gene58161:CDS
AAATTCTACAAATATCTACA+TGG 0.602639 4.2:+10121257 MS.gene58161:CDS
TCATCCACAGAAACACTAAG+AGG 0.607502 4.2:-10121551 None:intergenic
TAACTTACATCTTTCAGCCG+TGG 0.614000 4.2:-10121311 None:intergenic
GTGGATGAAGACCTCAACGA+TGG 0.630682 4.2:+10121566 MS.gene58161:CDS
CTTGTTTAGCCCCATCGTTG+AGG 0.638898 4.2:-10121577 None:intergenic
CTGTTTAACAGGGACAAATG+GGG 0.641129 4.2:+10121189 MS.gene58161:intron
AGATCGAATCAACCATGCCA+CGG 0.650410 4.2:+10121294 MS.gene58161:CDS
TCACTCCTGAATTGCTCCAG+CGG 0.651035 4.2:+10121757 MS.gene58161:CDS
TCTAGTCGCTTCATATCATG+TGG 0.661842 4.2:-10120068 None:intergenic
ACATGATATGAAGCGACTAG+AGG 0.666290 4.2:+10120070 MS.gene58161:CDS
AATGCCTTGCAAAATACTGG+TGG 0.667743 4.2:+10121813 MS.gene58161:CDS
ATACTGACAAGACCACCTGT+GGG 0.694097 4.2:-10121843 None:intergenic
GGATGAAGACCTCAACGATG+GGG 0.701949 4.2:+10121568 MS.gene58161:CDS
TATATACATATAATTGCACA+AGG 0.726498 4.2:+10119177 MS.gene58161:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAATAAATAAAAACAA+AGG - chr4.2:10120656-10120675 None:intergenic 10.0%
!! AAAGATACTAATAAGAAATT+TGG + chr4.2:10119391-10119410 MS.gene58161:intron 15.0%
!! AAGATACTAATAAGAAATTT+GGG + chr4.2:10119392-10119411 MS.gene58161:intron 15.0%
!! AATAAATACTTCAACTTTAT+GGG - chr4.2:10121099-10121118 None:intergenic 15.0%
!! AATATTGTCTCTATTTATAA+AGG + chr4.2:10121226-10121245 MS.gene58161:CDS 15.0%
!! ATAAAAAATAAAGCAAATGA+TGG - chr4.2:10121618-10121637 None:intergenic 15.0%
!! TATATGTATATAAGTATATG+AGG - chr4.2:10119169-10119188 None:intergenic 15.0%
!! TTTGTGTTATAAAATTTGAT+TGG + chr4.2:10120357-10120376 MS.gene58161:intron 15.0%
!!! AGATGAAATTCTATTTTAAA+TGG - chr4.2:10120266-10120285 None:intergenic 15.0%
!!! ATATTTTGAAAGATAAAATG+AGG - chr4.2:10119828-10119847 None:intergenic 15.0%
!!! ATTTTATGTTGTATTATTGT+AGG - chr4.2:10119542-10119561 None:intergenic 15.0%
!!! TTGAAATTTGTTAATTACAT+TGG + chr4.2:10120975-10120994 MS.gene58161:intron 15.0%
!!! TTTTATGTTGTATTATTGTA+GGG - chr4.2:10119541-10119560 None:intergenic 15.0%
!! AAATTCTACAAATATCTACA+TGG + chr4.2:10121257-10121276 MS.gene58161:CDS 20.0%
!! AGATACTAATAAGAAATTTG+GGG + chr4.2:10119393-10119412 MS.gene58161:intron 20.0%
!! ATAAATCAAGTAAAAAGGAA+AGG + chr4.2:10121949-10121968 MS.gene58161:CDS 20.0%
!! GAAATCCATAATTTATAGTT+GGG - chr4.2:10119754-10119773 None:intergenic 20.0%
!! GAATAAATACTTCAACTTTA+TGG - chr4.2:10121100-10121119 None:intergenic 20.0%
!! TAACAAATTTCAAAAGAATG+AGG - chr4.2:10120968-10120987 None:intergenic 20.0%
!! TATAACCCAACTATAAATTA+TGG + chr4.2:10119746-10119765 MS.gene58161:intron 20.0%
!! TATATAAGTATATGAGGATA+AGG - chr4.2:10119163-10119182 None:intergenic 20.0%
!! TATATACATATAATTGCACA+AGG + chr4.2:10119177-10119196 MS.gene58161:CDS 20.0%
!! TGAAAGATGTAAGTTAATAA+TGG + chr4.2:10121318-10121337 MS.gene58161:intron 20.0%
!! TGAAATCCATAATTTATAGT+TGG - chr4.2:10119755-10119774 None:intergenic 20.0%
!! TTCAAAATATAAGAAGTTAG+AGG + chr4.2:10119838-10119857 MS.gene58161:intron 20.0%
!!! TAAAAAGCACTATGAATAAA+AGG - chr4.2:10119939-10119958 None:intergenic 20.0%
!!! TTTCCTTTTTACTTGATTTA+TGG - chr4.2:10121950-10121969 None:intergenic 20.0%
! AAAACAATCGTGAATAGTTA+GGG - chr4.2:10120313-10120332 None:intergenic 25.0%
! AAAGAAGTTCAAGAATTACA+AGG + chr4.2:10119601-10119620 MS.gene58161:intron 25.0%
! AACTTTATGGGAAAAAAAGA+TGG - chr4.2:10121087-10121106 None:intergenic 25.0%
! AAGTTAAATGGCCTAAAAAA+TGG + chr4.2:10120502-10120521 MS.gene58161:intron 25.0%
! TAATAATTGCATCGAAGTTT+AGG + chr4.2:10120595-10120614 MS.gene58161:intron 25.0%
! TCATCATCAAGTAAAAAGAA+TGG - chr4.2:10120150-10120169 None:intergenic 25.0%
! TCATTGCTTGATATTATAAG+AGG + chr4.2:10121051-10121070 MS.gene58161:intron 25.0%
! TTTGATTGGATAAACTGAAA+TGG + chr4.2:10120371-10120390 MS.gene58161:intron 25.0%
!! AGACCCAAAAATCAATTTTA+CGG + chr4.2:10119983-10120002 MS.gene58161:intron 25.0%
!! GTATGTTCTATTTTCCTTTT+AGG - chr4.2:10119898-10119917 None:intergenic 25.0%
!! TTACAAGACCAAAAAAGTAT+CGG - chr4.2:10120178-10120197 None:intergenic 25.0%
!! TTCATCTCATTTTCTGATTT+TGG - chr4.2:10120472-10120491 None:intergenic 25.0%
!!! ACTAAACCTTGTTTTAAAGA+AGG - chr4.2:10119120-10119139 None:intergenic 25.0%
!!! TTTTTAGGCCATTTAACTTA+AGG - chr4.2:10120501-10120520 None:intergenic 25.0%
AAAATCTTACTCGTCAGTAT+TGG - chr4.2:10119802-10119821 None:intergenic 30.0%
AAATATGAGAGACACGATAT+AGG - chr4.2:10119214-10119233 None:intergenic 30.0%
AACTTCTTTCTTTCCTTGTT+GGG - chr4.2:10119590-10119609 None:intergenic 30.0%
AATATGAGAGACACGATATA+GGG - chr4.2:10119213-10119232 None:intergenic 30.0%
AATGGTTGAACATTGTGTAT+TGG + chr4.2:10121336-10121355 MS.gene58161:intron 30.0%
ACTTATTTACACTTCGTGTT+GGG - chr4.2:10119648-10119667 None:intergenic 30.0%
ATTAGATGTGGAAAGAAACA+AGG - chr4.2:10121663-10121682 None:intergenic 30.0%
CAACTTCAGTTATTTGTACA+CGG - chr4.2:10120570-10120589 None:intergenic 30.0%
CAGCCATAAATCAAGTAAAA+AGG + chr4.2:10121944-10121963 MS.gene58161:CDS 30.0%
CTGCAAGATTAATCAAGTAA+TGG - chr4.2:10120935-10120954 None:intergenic 30.0%
CTGTACAATACATATTAGGT+AGG - chr4.2:10120880-10120899 None:intergenic 30.0%
GAAAACAATCGTGAATAGTT+AGG - chr4.2:10120314-10120333 None:intergenic 30.0%
GTCATAAGAGTGATAAAAAG+CGG + chr4.2:10121907-10121926 MS.gene58161:CDS 30.0%
TACTTATTTACACTTCGTGT+TGG - chr4.2:10119649-10119668 None:intergenic 30.0%
TCTGGTAATTTCTCAAATGA+TGG + chr4.2:10120121-10120140 MS.gene58161:intron 30.0%
TTAAAACAAGGTTTAGTGTG+CGG + chr4.2:10119123-10119142 MS.gene58161:CDS 30.0%
TTGGCTGTACAATACATATT+AGG - chr4.2:10120884-10120903 None:intergenic 30.0%
TTGTATTATTGTAGGGCTTA+CGG - chr4.2:10119534-10119553 None:intergenic 30.0%
TTGTCAGTATAAAATCGAAC+CGG + chr4.2:10121856-10121875 MS.gene58161:CDS 30.0%
! ACAACTGCTGACAAATTTTT+GGG + chr4.2:10120023-10120042 MS.gene58161:CDS 30.0%
! ATGTAACTGGAATCTTTTCT+TGG - chr4.2:10120721-10120740 None:intergenic 30.0%
! GAGCTTCTAAAAATTAGATG+TGG - chr4.2:10121675-10121694 None:intergenic 30.0%
! TAAGTTCCATCTTTTTGACA+AGG - chr4.2:10121147-10121166 None:intergenic 30.0%
! TTAAAAGCACACCAAATCAA+TGG - chr4.2:10120621-10120640 None:intergenic 30.0%
!! CGTTTTCAGACTTTAATTTC+CGG - chr4.2:10121878-10121897 None:intergenic 30.0%
!! GCTCCGTAAAATTGATTTTT+GGG - chr4.2:10119989-10120008 None:intergenic 30.0%
!! TGCTCCGTAAAATTGATTTT+TGG - chr4.2:10119990-10120009 None:intergenic 30.0%
AAGTAATGGTAGTCAGCATT+TGG - chr4.2:10120921-10120940 None:intergenic 35.0%
ACAAGGATGGGTATTATCTT+AGG - chr4.2:10121646-10121665 None:intergenic 35.0%
ACATCACTGGATTACTGTAT+AGG + chr4.2:10121704-10121723 MS.gene58161:intron 35.0%
ATAGCGTATGTTGTAATGAG+TGG - chr4.2:10119729-10119748 None:intergenic 35.0%
ATGTCCAATGCTACAACATA+AGG - chr4.2:10120541-10120560 None:intergenic 35.0%
ATGTGGAAAGAAACAAGGAT+GGG - chr4.2:10121658-10121677 None:intergenic 35.0%
CAAGTAAAAAGGAAAGGAGA+AGG + chr4.2:10121955-10121974 MS.gene58161:CDS 35.0%
CCAACAGGGAAGTAATTAAA+CGG - chr4.2:10121122-10121141 None:intergenic 35.0%
CTTATTTACACTTCGTGTTG+GGG - chr4.2:10119647-10119666 None:intergenic 35.0%
GAACTTCTTTCTTTCCTTGT+TGG - chr4.2:10119591-10119610 None:intergenic 35.0%
GCAAGTACTTCTGTTTAACA+GGG + chr4.2:10121179-10121198 MS.gene58161:intron 35.0%
GGATGAAATATGCTCCTAAA+AGG + chr4.2:10119881-10119900 MS.gene58161:intron 35.0%
TACTGTATAGGACGATATGA+AGG + chr4.2:10121716-10121735 MS.gene58161:intron 35.0%
TCAATATGTGAGAACTGTGA+TGG + chr4.2:10120445-10120464 MS.gene58161:intron 35.0%
TCTGTTTAACAGGGACAAAT+GGG + chr4.2:10121188-10121207 MS.gene58161:intron 35.0%
TGCAAGTACTTCTGTTTAAC+AGG + chr4.2:10121178-10121197 MS.gene58161:intron 35.0%
TGTAATGAGTGGAACTATGA+CGG - chr4.2:10119718-10119737 None:intergenic 35.0%
TTAAAGTCTGAAAACGAGCA+TGG + chr4.2:10121882-10121901 MS.gene58161:CDS 35.0%
TTCTGTTTAACAGGGACAAA+TGG + chr4.2:10121187-10121206 MS.gene58161:intron 35.0%
! GACAACTGCTGACAAATTTT+TGG + chr4.2:10120022-10120041 MS.gene58161:CDS 35.0%
! GAGAAAAAGTACATAACCTG+CGG + chr4.2:10119860-10119879 MS.gene58161:intron 35.0%
! GCTCTTATCTTTTACATCAC+TGG + chr4.2:10121691-10121710 MS.gene58161:intron 35.0%
! TCGAGGACGAAATTCTTTTA+GGG - chr4.2:10119435-10119454 None:intergenic 35.0%
! TGAGCATGTGATAATGTAAC+TGG - chr4.2:10120734-10120753 None:intergenic 35.0%
! TTCGAGGACGAAATTCTTTT+AGG - chr4.2:10119436-10119455 None:intergenic 35.0%
! TTTTCCACCAGTATTTTGCA+AGG - chr4.2:10121820-10121839 None:intergenic 35.0%
! TTTTTCTTTTCCCCCTTTGT+CGG + chr4.2:10121494-10121513 MS.gene58161:intron 35.0%
!! ATGCTTGTTTGTTGTTGTCA+TGG - chr4.2:10119674-10119693 None:intergenic 35.0%
!! GAATCTTTTCTTGGAAGTAC+AGG - chr4.2:10120712-10120731 None:intergenic 35.0%
!! GGCTATCCTTCTTTAAAACA+AGG + chr4.2:10119111-10119130 MS.gene58161:CDS 35.0%
AAACAGAAGAAACCCGACAA+AGG - chr4.2:10121510-10121529 None:intergenic 40.0%
AACAGAAGAAACCCGACAAA+GGG - chr4.2:10121509-10121528 None:intergenic 40.0%
AACAGGCTCAAGATTAGTGA+AGG - chr4.2:10121476-10121495 None:intergenic 40.0%
AAGAGGTTCCATGACAATCT+AGG - chr4.2:10121537-10121556 None:intergenic 40.0%
AAGCCCTGCCTTAAGTTAAA+TGG + chr4.2:10120490-10120509 MS.gene58161:intron 40.0%
AATGCCTTGCAAAATACTGG+TGG + chr4.2:10121813-10121832 MS.gene58161:CDS 40.0%
AATGGCCTAAAAAATGGTCC+AGG + chr4.2:10120508-10120527 MS.gene58161:intron 40.0%
ACATGATATGAAGCGACTAG+AGG + chr4.2:10120070-10120089 MS.gene58161:CDS 40.0%
AGAGGTTCCATGACAATCTA+GGG - chr4.2:10121536-10121555 None:intergenic 40.0%
CAAAGGGGGAAAAGAAAAAC+AGG - chr4.2:10121493-10121512 None:intergenic 40.0%
CCGTTTAATTACTTCCCTGT+TGG + chr4.2:10121119-10121138 MS.gene58161:intron 40.0%
CTGTTTAACAGGGACAAATG+GGG + chr4.2:10121189-10121208 MS.gene58161:intron 40.0%
GAATTTCGTCCTCGAAATTC+AGG + chr4.2:10119440-10119459 MS.gene58161:intron 40.0%
GACAATGCCTTGCAAAATAC+TGG + chr4.2:10121810-10121829 MS.gene58161:CDS 40.0%
GATGTGGAAAGAAACAAGGA+TGG - chr4.2:10121659-10121678 None:intergenic 40.0%
GCTAGATTCTCCATCTTCAA+AGG - chr4.2:10121787-10121806 None:intergenic 40.0%
GTAATGAGTGGAACTATGAC+GGG - chr4.2:10119717-10119736 None:intergenic 40.0%
GTTGCCTTATGTTGTAGCAT+TGG + chr4.2:10120534-10120553 MS.gene58161:intron 40.0%
TAACTTACATCTTTCAGCCG+TGG - chr4.2:10121314-10121333 None:intergenic 40.0%
TCATCCACAGAAACACTAAG+AGG - chr4.2:10121554-10121573 None:intergenic 40.0%
TCTAGTCGCTTCATATCATG+TGG - chr4.2:10120071-10120090 None:intergenic 40.0%
TTCCTTGTTGGGAGTCTATA+GGG - chr4.2:10119579-10119598 None:intergenic 40.0%
TTGTCAGCAGTTGTCTTGAT+AGG - chr4.2:10120017-10120036 None:intergenic 40.0%
TTTCCTTGTTGGGAGTCTAT+AGG - chr4.2:10119580-10119599 None:intergenic 40.0%
TTTGGTTGTTCTTCGTCACT+TGG - chr4.2:10120903-10120922 None:intergenic 40.0%
! AGGCCATTTAACTTAAGGCA+GGG - chr4.2:10120496-10120515 None:intergenic 40.0%
! CGAAGTTTAGGCCATTGATT+TGG + chr4.2:10120607-10120626 MS.gene58161:intron 40.0%
! CGAGGACGAAATTCTTTTAG+GGG - chr4.2:10119434-10119453 None:intergenic 40.0%
! CGTGAATAGTTAGGGTGTTT+GGG - chr4.2:10120305-10120324 None:intergenic 40.0%
! GAGGACGAAATTCTTTTAGG+GGG - chr4.2:10119433-10119452 None:intergenic 40.0%
! GTGAATAGTTAGGGTGTTTG+GGG - chr4.2:10120304-10120323 None:intergenic 40.0%
! GTTTTGTCCCTAGATTGTCA+TGG + chr4.2:10121526-10121545 MS.gene58161:intron 40.0%
! TAGGCCATTTAACTTAAGGC+AGG - chr4.2:10120497-10120516 None:intergenic 40.0%
! TCGTGAATAGTTAGGGTGTT+TGG - chr4.2:10120306-10120325 None:intergenic 40.0%
! TCTACATGGTCTTGCATTCA+AGG + chr4.2:10121271-10121290 MS.gene58161:CDS 40.0%
! TGAATAGTTAGGGTGTTTGG+GGG - chr4.2:10120303-10120322 None:intergenic 40.0%
! TTGGTGCCTTGTCAAAAAGA+TGG + chr4.2:10121138-10121157 MS.gene58161:intron 40.0%
! TTTTCTTTTCCCCCTTTGTC+GGG + chr4.2:10121495-10121514 MS.gene58161:intron 40.0%
!! AGACCAAAAAAGTATCGGAC+TGG - chr4.2:10120173-10120192 None:intergenic 40.0%
!! AGGACGAAATTCTTTTAGGG+GGG - chr4.2:10119432-10119451 None:intergenic 40.0%
!! CAATCTTGCTGACTTTCATC+TGG + chr4.2:10120103-10120122 MS.gene58161:CDS 40.0%
!! GTCAGTATTGGTGCTCTATT+TGG - chr4.2:10119790-10119809 None:intergenic 40.0%
!! TTTTTGACAAGGCACCAACA+GGG - chr4.2:10121136-10121155 None:intergenic 40.0%
!!! GATTTTTGGGTCTATGATGC+TGG - chr4.2:10119976-10119995 None:intergenic 40.0%
!!! TGACCAGTCCGATACTTTTT+TGG + chr4.2:10120167-10120186 MS.gene58161:intron 40.0%
AAGAGAGAGTGAGAACAGCT+AGG - chr4.2:10120229-10120248 None:intergenic 45.0%
ACAGAAGAAACCCGACAAAG+GGG - chr4.2:10121508-10121527 None:intergenic 45.0%
ACTGGTGGAAAAATACCCAC+AGG + chr4.2:10121828-10121847 MS.gene58161:CDS 45.0%
AGATCGAATCAACCATGCCA+CGG + chr4.2:10121294-10121313 MS.gene58161:CDS 45.0%
ATACTGACAAGACCACCTGT+GGG - chr4.2:10121846-10121865 None:intergenic 45.0%
GAACAGCTAGGACTTAAGAG+TGG - chr4.2:10120217-10120236 None:intergenic 45.0%
GAGTGATAAAAAGCGGCGTA+TGG + chr4.2:10121914-10121933 MS.gene58161:CDS 45.0%
GCCTAAAAAATGGTCCAGGA+CGG + chr4.2:10120512-10120531 MS.gene58161:intron 45.0%
TATACTGACAAGACCACCTG+TGG - chr4.2:10121847-10121866 None:intergenic 45.0%
TCAGGACATTTCGCACATTG+AGG + chr4.2:10120787-10120806 MS.gene58161:CDS 45.0%
TCCTTGTTGGGAGTCTATAG+GGG - chr4.2:10119578-10119597 None:intergenic 45.0%
TGAGAGACACGATATAGGGA+TGG - chr4.2:10119209-10119228 None:intergenic 45.0%
TGGATGAAGACCTCAACGAT+GGG + chr4.2:10121567-10121586 MS.gene58161:CDS 45.0%
TTAACAGGGACAAATGGGGT+TGG + chr4.2:10121193-10121212 MS.gene58161:intron 45.0%
TTCTCGTGTCCTGAATTTCG+AGG - chr4.2:10119452-10119471 None:intergenic 45.0%
! ATGCAAGTGCTGCAGTATGT+AGG + chr4.2:10121410-10121429 MS.gene58161:intron 45.0%
! GGAACCTCTTAGTGTTTCTG+TGG + chr4.2:10121547-10121566 MS.gene58161:CDS 45.0%
! TAGGAGCATATTTCATCCGC+AGG - chr4.2:10119879-10119898 None:intergenic 45.0%
! TGCAAGTGCTGCAGTATGTA+GGG + chr4.2:10121411-10121430 MS.gene58161:intron 45.0%
!! CTTTTTGACAAGGCACCAAC+AGG - chr4.2:10121137-10121156 None:intergenic 45.0%
!!! ACCGTCCTGGACCATTTTTT+AGG - chr4.2:10120516-10120535 None:intergenic 45.0%
!!! GAATATTAATTTAATTTTTA+TGG - chr4.2:10120397-10120416 None:intergenic 5.0%
ACAACATAAGGCAACCGTCC+TGG - chr4.2:10120529-10120548 None:intergenic 50.0%
AGGGACAAATGGGGTTGGAA+AGG + chr4.2:10121198-10121217 MS.gene58161:intron 50.0%
CAGAAGAAACCCGACAAAGG+GGG - chr4.2:10121507-10121526 None:intergenic 50.0%
CGATGGGGCTAAACAAGTTG+AGG + chr4.2:10121583-10121602 MS.gene58161:CDS 50.0%
CTTGTTTAGCCCCATCGTTG+AGG - chr4.2:10121580-10121599 None:intergenic 50.0%
GGATGAAGACCTCAACGATG+GGG + chr4.2:10121568-10121587 MS.gene58161:CDS 50.0%
GGTGGAAAAATACCCACAGG+TGG + chr4.2:10121831-10121850 MS.gene58161:CDS 50.0%
GTGGATGAAGACCTCAACGA+TGG + chr4.2:10121566-10121585 MS.gene58161:CDS 50.0%
TACATCTTTCAGCCGTGGCA+TGG - chr4.2:10121309-10121328 None:intergenic 50.0%
TACTGCAGCACTTGCATGTG+AGG - chr4.2:10121407-10121426 None:intergenic 50.0%
TCACTCCTGAATTGCTCCAG+CGG + chr4.2:10121757-10121776 MS.gene58161:CDS 50.0%
TCCCCTATAGACTCCCAACA+AGG + chr4.2:10119574-10119593 MS.gene58161:intron 50.0%
! AAGTACAGGTGCGAAAGTGC+AGG - chr4.2:10120698-10120717 None:intergenic 50.0%
! AGTTAGGGTGTTTGGGGGAA+GGG - chr4.2:10120298-10120317 None:intergenic 50.0%
! CAGCGGTATGCCTTTGAAGA+TGG + chr4.2:10121774-10121793 MS.gene58161:CDS 50.0%
! CATCTTCAAAGGCATACCGC+TGG - chr4.2:10121776-10121795 None:intergenic 50.0%
! GCTTGTGTGTTACTCTGCAC+AGG + chr4.2:10120755-10120774 MS.gene58161:CDS 50.0%
! TAGTTAGGGTGTTTGGGGGA+AGG - chr4.2:10120299-10120318 None:intergenic 50.0%
!! TGAGTGGAACTATGACGGGT+TGG - chr4.2:10119713-10119732 None:intergenic 50.0%
CTGCACAGGTCTGCAGAATC+AGG + chr4.2:10120769-10120788 MS.gene58161:CDS 55.0%
GCATACCGCTGGAGCAATTC+AGG - chr4.2:10121765-10121784 None:intergenic 55.0%
! GTTAGGGTGTTTGGGGGAAG+GGG - chr4.2:10120297-10120316 None:intergenic 55.0%
! TTAGGGTGTTTGGGGGAAGG+GGG - chr4.2:10120296-10120315 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 10119108 10121983 10119108 ID=MS.gene58161
chr4.2 mRNA 10119108 10121983 10119108 ID=MS.gene58161.t1;Parent=MS.gene58161
chr4.2 exon 10119108 10119198 10119108 ID=MS.gene58161.t1.exon1;Parent=MS.gene58161.t1
chr4.2 CDS 10119108 10119198 10119108 ID=cds.MS.gene58161.t1;Parent=MS.gene58161.t1
chr4.2 exon 10119985 10120124 10119985 ID=MS.gene58161.t1.exon2;Parent=MS.gene58161.t1
chr4.2 CDS 10119985 10120124 10119985 ID=cds.MS.gene58161.t1;Parent=MS.gene58161.t1
chr4.2 exon 10120680 10120808 10120680 ID=MS.gene58161.t1.exon3;Parent=MS.gene58161.t1
chr4.2 CDS 10120680 10120808 10120680 ID=cds.MS.gene58161.t1;Parent=MS.gene58161.t1
chr4.2 exon 10121200 10121325 10121200 ID=MS.gene58161.t1.exon4;Parent=MS.gene58161.t1
chr4.2 CDS 10121200 10121325 10121200 ID=cds.MS.gene58161.t1;Parent=MS.gene58161.t1
chr4.2 exon 10121539 10121604 10121539 ID=MS.gene58161.t1.exon5;Parent=MS.gene58161.t1
chr4.2 CDS 10121539 10121604 10121539 ID=cds.MS.gene58161.t1;Parent=MS.gene58161.t1
chr4.2 exon 10121726 10121983 10121726 ID=MS.gene58161.t1.exon6;Parent=MS.gene58161.t1
chr4.2 CDS 10121726 10121983 10121726 ID=cds.MS.gene58161.t1;Parent=MS.gene58161.t1
Gene Sequence

>MS.gene58161

ATGGGCTATCCTTCTTTAAAACAAGGTTTAGTGTGCGGAACAAGTTTTCTAACCTTATCCTCATATACTTATATACATATAATTGCACAAGGTAAACACCATCCCTATATCGTGTCTCTCATATTTAATATTATATTATTATTCTATTCAAAACAAGAAATTCTATCTAATTATTACTTTTAAATATTTTAGTTTATTAACATTTACATAGATTAAGACAAGCTTACTTCTAGAAAGTTTTAGTGAACAATAATAGTAATAATAATTTATCATGATATTATAAAAAGATACTAATAAGAAATTTGGGGTGTTACAACTCTACCCCCCTAAAAGAATTTCGTCCTCGAAATTCAGGACACGAGAAAATAAATTTAAGTATTGCTCAAATACATTAAACTATAAAGTTTTTCAAACACTAACTCACCGTAAGCCCTACAATAATACAACATAAAATCAAAACAAATTTTCCCCTATAGACTCCCAACAAGGAAAGAAAGAAGTTCAAGAATTACAAGGCGAATAAATCAGTCATAAAGCCCCAACACGAAGTGTAAATAAGTAAACCATGACAACAACAAACAAGCATATGAAAATAAGAGCTACCAACCCGTCATAGTTCCACTCATTACAACATACGCTATAACCCAACTATAAATTATGGATTTCAATTAATAGAGAACCAAATAGAGCACCAATACTGACGAGTAAGATTTTTCACCTCATTTTATCTTTCAAAATATAAGAAGTTAGAGGAGAAAAAGTACATAACCTGCGGATGAAATATGCTCCTAAAAGGAAAATAGAACATACAGAATTTTATTCATAGTGCCTTTTATTCATAGTGCTTTTTATTTCGATAATGTATCCAGCATCATAGACCCAAAAATCAATTTTACGGAGCAAGAGCCTATCAAGACAACTGCTGACAAATTTTTGGGATCAGTTAAAGAAAATTTATCACCACATGATATGAAGCGACTAGAGGCTTATGTTGACAATCTTGCTGACTTTCATCTGGTAATTTCTCAAATGATGGATACCATTCTTTTTACTTGATGATGACCAGTCCGATACTTTTTTGGTCTTGTAAACTTGCTATGTGTACTCCACTCTTAAGTCCTAGCTGTTCTCACTCTCTCTTGATAATAGATGATTCCATTTAAAATAGAATTTCATCTTGCTTCTCCCCCTTCCCCCAAACACCCTAACTATTCACGATTGTTTTCTTACAGAAGTATATATCTTTAAGTTTGTGTTATAAAATTTGATTGGATAAACTGAAATGGCCATAAAAATTAAATTAATATTCACTTGCGTTTGCTTCAAGTTCATGAGCATCAATATGTGAGAACTGTGATGGACCAAAATCAGAAAATGAGATGAAGCCCTGCCTTAAGTTAAATGGCCTAAAAAATGGTCCAGGACGGTTGCCTTATGTTGTAGCATTGGACATTATGCACCGTGTACAAATAACTGAAGTTGTGATGTAATAATTGCATCGAAGTTTAGGCCATTGATTTGGTGTGCTTTTAAGTATGCTCTTAACCTTTGTTTTTATTTATTTTTTGACAGATTTTAGATTTAGTTCCTGCACTTTCGCACCTGTACTTCCAAGAAAAGATTCCAGTTACATTATCACATGCTCAAGCTTGTGTGTTACTCTGCACAGGTCTGCAGAATCAGGACATTTCGCACATTGAGGTCTGCTCACTAACTTGCAACATTTTTTGTTGAAATAACACAATCTTGTGATTATCTAGCAGTTTGCACCTACCTAATATGTATTGTACAGCCAAGTGACGAAGAACAACCAAATGCTGACTACCATTACTTGATTAATCTTGCAGAAAATTTTATCCTCATTCTTTTGAAATTTGTTAATTACATTGGTCAATTCATTATGATAACAAATTGTTTATTAAATCAACGAATAACTCTAAAATTCATTGCTTGATATTATAAGAGGTTTGAATAGACCATCTTTTTTTCCCATAAAGTTGAAGTATTTATTCCGTTTAATTACTTCCCTGTTGGTGCCTTGTCAAAAAGATGGAACTTAATTCTCATTTGTGCAAGTACTTCTGTTTAACAGGGACAAATGGGGTTGGAAAGGCAACAAATATTGTCTCTATTTATAAAGGTTATGAAGAAATTCTACAAATATCTACATGGTCTTGCATTCAAGGAGATCGAATCAACCATGCCACGGCTGAAAGATGTAAGTTAATAATGGTTGAACATTGTGTATTGGCTTGATGTTTTCACTGAATTTCGTAGCAAGTTGAGTTAATGAAATCCTCACATGCAAGTGCTGCAGTATGTAGGGACACAAATTCTACTAATTTGCAGCAGCTTTGCTTTTTATCCTTCACTAATCTTGAGCCTGTTTTTCTTTTCCCCCTTTGTCGGGTTTCTTCTGTTTTGTCCCTAGATTGTCATGGAACCTCTTAGTGTTTCTGTGGATGAAGACCTCAACGATGGGGCTAAACAAGTTGAGGTATGTACTTCCATCATTTGCTTTATTTTTTATAAGATCCTAAGATAATACCCATCCTTGTTTCTTTCCACATCTAATTTTTAGAAGCTCTTATCTTTTACATCACTGGATTACTGTATAGGACGATATGAAGGCAAAATCAGATTCTCTCTTCACTCCTGAATTGCTCCAGCGGTATGCCTTTGAAGATGGAGAATCTAGCTTTGACAATGCCTTGCAAAATACTGGTGGAAAAATACCCACAGGTGGTCTTGTCAGTATAAAATCGAACCGGAAATTAAAGTCTGAAAACGAGCATGGAAGTCATAAGAGTGATAAAAAGCGGCGTATGGACAATCACAGCCATAAATCAAGTAAAAAGGAAAGGAGAAGGTCTTGA
Protein sequence

>MS.gene58161.t1

MGYPSLKQGLVCGTSFLTLSSYTYIHIIAQDPKINFTEQEPIKTTADKFLGSVKENLSPHDMKRLEAYVDNLADFHLILDLVPALSHLYFQEKIPVTLSHAQACVLLCTGLQNQDISHIEGQMGLERQQILSLFIKVMKKFYKYLHGLAFKEIESTMPRLKDIVMEPLSVSVDEDLNDGAKQVEDDMKAKSDSLFTPELLQRYAFEDGESSFDNALQNTGGKIPTGGLVSIKSNRKLKSENEHGSHKSDKKRRMDNHSHKSSKKERRRS