Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58303.t1 | XP_013444914.1 | 89.7 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 2.00E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58303.t1 | A0A072TN78 | 89.7 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 1.5e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050410 | MS.gene58303 | 0.800821 | 1.20E-48 | -1.69E-46 |
| MS.gene055150 | MS.gene58303 | 0.827541 | 1.48E-54 | -1.69E-46 |
| MS.gene055886 | MS.gene58303 | 0.800879 | 1.17E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58303.t1 | MTR_8g432440 | 89.674 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.96e-121 | 339 |
| MS.gene58303.t1 | MTR_8g432420 | 89.674 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.96e-121 | 339 |
| MS.gene58303.t1 | MTR_8g432460 | 87.978 | 183 | 22 | 0 | 1 | 183 | 1 | 183 | 1.77e-119 | 335 |
| MS.gene58303.t1 | MTR_8g432470 | 86.413 | 184 | 24 | 1 | 1 | 183 | 1 | 184 | 1.74e-115 | 325 |
| MS.gene58303.t1 | MTR_8g432490 | 83.607 | 183 | 30 | 0 | 1 | 183 | 1 | 183 | 1.27e-112 | 318 |
| MS.gene58303.t1 | MTR_8g432450 | 85.876 | 177 | 23 | 2 | 1 | 175 | 1 | 177 | 3.02e-107 | 304 |
| MS.gene58303.t1 | MTR_3g064780 | 80.556 | 180 | 35 | 0 | 1 | 180 | 1 | 180 | 8.93e-107 | 306 |
| MS.gene58303.t1 | MTR_8g432480 | 53.073 | 179 | 56 | 5 | 5 | 174 | 7 | 166 | 8.37e-50 | 159 |
| MS.gene58303.t1 | MTR_7g076000 | 34.078 | 179 | 90 | 6 | 5 | 176 | 2 | 159 | 5.65e-23 | 90.1 |
| MS.gene58303.t1 | MTR_8g432370 | 35.106 | 188 | 102 | 7 | 5 | 183 | 2 | 178 | 3.69e-22 | 88.6 |
| MS.gene58303.t1 | MTR_8g432400 | 33.690 | 187 | 107 | 6 | 5 | 183 | 2 | 179 | 1.71e-21 | 86.7 |
| MS.gene58303.t1 | MTR_8g432350 | 35.165 | 182 | 98 | 7 | 5 | 177 | 2 | 172 | 7.75e-21 | 85.1 |
| MS.gene58303.t1 | MTR_8g432390 | 34.921 | 189 | 102 | 8 | 5 | 183 | 2 | 179 | 3.81e-20 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTTATAAATTGAGGAAT+TGG | 0.246315 | 8.2:-65528039 | MS.gene58303:CDS |
| AATTATGGTGGTCGGTTAAC+TGG | 0.256041 | 8.2:-65527914 | MS.gene58303:CDS |
| TCAAAGGTATTCGACCTATT+GGG | 0.300168 | 8.2:-65528126 | MS.gene58303:CDS |
| TTCAAAGGTATTCGACCTAT+TGG | 0.303554 | 8.2:-65528127 | MS.gene58303:CDS |
| TTGCTATTGGTATTCCCTTT+TGG | 0.313784 | 8.2:-65527859 | MS.gene58303:CDS |
| AATTGGGAAGGGAGGCAAAA+TGG | 0.332260 | 8.2:-65528022 | MS.gene58303:CDS |
| TTCACAAACATTAGAAATTA+CGG | 0.337907 | 8.2:-65528353 | MS.gene58303:CDS |
| TTAGAAATTACGGTTATATC+AGG | 0.339630 | 8.2:-65528343 | MS.gene58303:CDS |
| GTGGCGGATGGAAAGAATTA+TGG | 0.368827 | 8.2:-65527929 | MS.gene58303:CDS |
| GCAATTCTTGCCACCCCAAT+AGG | 0.391470 | 8.2:+65528112 | None:intergenic |
| GACTCTAATGGTGTTGCTAT+TGG | 0.404474 | 8.2:-65527872 | MS.gene58303:CDS |
| GGTGGCAAGAATTGCTCTTT+CGG | 0.416259 | 8.2:-65528104 | MS.gene58303:CDS |
| GCAACACCATTAGAGTCCTT+TGG | 0.425939 | 8.2:+65527878 | None:intergenic |
| CACCGTTTGCTTCTCCGCCT+CGG | 0.429544 | 8.2:+65527949 | None:intergenic |
| GAGTTATAAATTGAGGAATT+GGG | 0.456017 | 8.2:-65528038 | MS.gene58303:CDS |
| TATTCTCAATGGCGCGGTAT+CGG | 0.469676 | 8.2:-65528080 | MS.gene58303:CDS |
| GCTCTTTCGGATATTCTCAA+TGG | 0.472815 | 8.2:-65528091 | MS.gene58303:CDS |
| TTTGGGTAATTCCACCAAAA+GGG | 0.478388 | 8.2:+65527845 | None:intergenic |
| TGAAGGAAAATGATAATTGT+GGG | 0.480545 | 8.2:-65528243 | MS.gene58303:CDS |
| GGCGGAGAAGCAAACGGTGG+CGG | 0.482611 | 8.2:-65527945 | MS.gene58303:CDS |
| GTTGGTCCAAAGGACTCTAA+TGG | 0.484161 | 8.2:-65527884 | MS.gene58303:CDS |
| GTGAAGGAAAATGATAATTG+TGG | 0.484213 | 8.2:-65528244 | MS.gene58303:CDS |
| TAGTGTGAGGGTGGTTGTGC+CGG | 0.504410 | 8.2:-65527987 | MS.gene58303:CDS |
| TGCAAGGTCAATCACGTTTG+AGG | 0.523501 | 8.2:-65528164 | MS.gene58303:CDS |
| CAAAGGTATTCGACCTATTG+GGG | 0.532466 | 8.2:-65528125 | MS.gene58303:CDS |
| GAGAAGCAAACGGTGGCGGA+TGG | 0.538736 | 8.2:-65527941 | MS.gene58303:CDS |
| CGGTTAACTGGAATGGATGT+TGG | 0.551714 | 8.2:-65527902 | MS.gene58303:CDS |
| TGGTGGTCGGTTAACTGGAA+TGG | 0.553085 | 8.2:-65527909 | MS.gene58303:CDS |
| AATTGAGGAATTGGGAAGGG+AGG | 0.560763 | 8.2:-65528030 | MS.gene58303:CDS |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.2:-65528180 | MS.gene58303:CDS |
| GATGCATACGTTGTTGTTCG+AGG | 0.567581 | 8.2:-65528298 | MS.gene58303:CDS |
| TATAAATTGAGGAATTGGGA+AGG | 0.569289 | 8.2:-65528034 | MS.gene58303:CDS |
| GGTTGTGCCGGAGCAAAGAT+CGG | 0.574606 | 8.2:-65527975 | MS.gene58303:CDS |
| ATAAATTGAGGAATTGGGAA+GGG | 0.583144 | 8.2:-65528033 | MS.gene58303:CDS |
| TACCGAGGCGGAGAAGCAAA+CGG | 0.584351 | 8.2:-65527951 | MS.gene58303:CDS |
| AGGCGAAAATATTCATGTAA+CGG | 0.589149 | 8.2:-65528323 | MS.gene58303:CDS |
| TGGAATGGATGTTGGTCCAA+AGG | 0.593717 | 8.2:-65527894 | MS.gene58303:CDS |
| GTGCAATGCAAGAAGTTCAA+AGG | 0.594547 | 8.2:-65528142 | MS.gene58303:CDS |
| TTCGGATATTCTCAATGGCG+CGG | 0.599946 | 8.2:-65528086 | MS.gene58303:CDS |
| TCGGTAACCGATCTTTGCTC+CGG | 0.614768 | 8.2:+65527968 | None:intergenic |
| GTGATTGACCTTGCATGCAA+TGG | 0.618595 | 8.2:+65528172 | None:intergenic |
| ATGGAAAGAATTATGGTGGT+CGG | 0.623818 | 8.2:-65527922 | MS.gene58303:CDS |
| TGTTTGTGAAGCTACAACCA+TGG | 0.632825 | 8.2:+65528366 | None:intergenic |
| CGAAAATATTCATGTAACGG+AGG | 0.635995 | 8.2:-65528320 | MS.gene58303:CDS |
| GCAAAGATCGGTTACCGAGG+CGG | 0.653482 | 8.2:-65527963 | MS.gene58303:CDS |
| GCGGATGGAAAGAATTATGG+TGG | 0.668878 | 8.2:-65527926 | MS.gene58303:CDS |
| AGGTATTCGACCTATTGGGG+TGG | 0.674947 | 8.2:-65528122 | MS.gene58303:CDS |
| GGAGCAAAGATCGGTTACCG+AGG | 0.696731 | 8.2:-65527966 | MS.gene58303:CDS |
| CGAGGCGGAGAAGCAAACGG+TGG | 0.746726 | 8.2:-65527948 | MS.gene58303:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAGTTACACAACAAAAA+CGG | - | chr8.2:65527933-65527952 | MS.gene58303:CDS | 20.0% |
| !! | TTCACAAACATTAGAAATTA+CGG | - | chr8.2:65527846-65527865 | MS.gene58303:CDS | 20.0% |
| !!! | GAATTTTGAGTTATAAATTG+AGG | - | chr8.2:65528154-65528173 | MS.gene58303:CDS | 20.0% |
| ! | GAGTTATAAATTGAGGAATT+GGG | - | chr8.2:65528161-65528180 | MS.gene58303:CDS | 25.0% |
| ! | TGAAGGAAAATGATAATTGT+GGG | - | chr8.2:65527956-65527975 | MS.gene58303:CDS | 25.0% |
| ! | TGAGTTATAAATTGAGGAAT+TGG | - | chr8.2:65528160-65528179 | MS.gene58303:CDS | 25.0% |
| ! | TTAGAAATTACGGTTATATC+AGG | - | chr8.2:65527856-65527875 | MS.gene58303:CDS | 25.0% |
| !!! | AAAACTCGAGTTTTATTTCA+TGG | - | chr8.2:65527980-65527999 | MS.gene58303:CDS | 25.0% |
| !!! | AGTTATTCATTTTAGTGTGA+GGG | - | chr8.2:65528200-65528219 | MS.gene58303:CDS | 25.0% |
| AGGCGAAAATATTCATGTAA+CGG | - | chr8.2:65527876-65527895 | MS.gene58303:CDS | 30.0% | |
| ATAAATTGAGGAATTGGGAA+GGG | - | chr8.2:65528166-65528185 | MS.gene58303:CDS | 30.0% | |
| GTGAAGGAAAATGATAATTG+TGG | - | chr8.2:65527955-65527974 | MS.gene58303:CDS | 30.0% | |
| TATAAATTGAGGAATTGGGA+AGG | - | chr8.2:65528165-65528184 | MS.gene58303:CDS | 30.0% | |
| !! | GAGTTATTCATTTTAGTGTG+AGG | - | chr8.2:65528199-65528218 | MS.gene58303:CDS | 30.0% |
| ATGGAAAGAATTATGGTGGT+CGG | - | chr8.2:65528277-65528296 | MS.gene58303:CDS | 35.0% | |
| CGAAAATATTCATGTAACGG+AGG | - | chr8.2:65527879-65527898 | MS.gene58303:CDS | 35.0% | |
| TTACACAACAAAAACGGTGA+AGG | - | chr8.2:65527939-65527958 | MS.gene58303:CDS | 35.0% | |
| TTTGGGTAATTCCACCAAAA+GGG | + | chr8.2:65528357-65528376 | None:intergenic | 35.0% | |
| !! | TATTCATTTTAGTGTGAGGG+TGG | - | chr8.2:65528203-65528222 | MS.gene58303:CDS | 35.0% |
| !! | TCAAAGGTATTCGACCTATT+GGG | - | chr8.2:65528073-65528092 | MS.gene58303:CDS | 35.0% |
| !! | TTCAAAGGTATTCGACCTAT+TGG | - | chr8.2:65528072-65528091 | MS.gene58303:CDS | 35.0% |
| !! | TTGCTATTGGTATTCCCTTT+TGG | - | chr8.2:65528340-65528359 | MS.gene58303:CDS | 35.0% |
| !! | TTTTGGGTAATTCCACCAAA+AGG | + | chr8.2:65528358-65528377 | None:intergenic | 35.0% |
| AATTATGGTGGTCGGTTAAC+TGG | - | chr8.2:65528285-65528304 | MS.gene58303:CDS | 40.0% | |
| GCTCTTTCGGATATTCTCAA+TGG | - | chr8.2:65528108-65528127 | MS.gene58303:CDS | 40.0% | |
| GTGCAATGCAAGAAGTTCAA+AGG | - | chr8.2:65528057-65528076 | MS.gene58303:CDS | 40.0% | |
| ! | CTATTGGTATTCCCTTTTGG+TGG | - | chr8.2:65528343-65528362 | MS.gene58303:CDS | 40.0% |
| !! | CAAAGGTATTCGACCTATTG+GGG | - | chr8.2:65528074-65528093 | MS.gene58303:CDS | 40.0% |
| !! | GACTCTAATGGTGTTGCTAT+TGG | - | chr8.2:65528327-65528346 | MS.gene58303:CDS | 40.0% |
| AATTGAGGAATTGGGAAGGG+AGG | - | chr8.2:65528169-65528188 | MS.gene58303:CDS | 45.0% | |
| AATTGGGAAGGGAGGCAAAA+TGG | - | chr8.2:65528177-65528196 | MS.gene58303:CDS | 45.0% | |
| CGGTTAACTGGAATGGATGT+TGG | - | chr8.2:65528297-65528316 | MS.gene58303:CDS | 45.0% | |
| GCAACACCATTAGAGTCCTT+TGG | + | chr8.2:65528324-65528343 | None:intergenic | 45.0% | |
| GCGGATGGAAAGAATTATGG+TGG | - | chr8.2:65528273-65528292 | MS.gene58303:CDS | 45.0% | |
| GGTGGCAAGAATTGCTCTTT+CGG | - | chr8.2:65528095-65528114 | MS.gene58303:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.2:65528030-65528049 | None:intergenic | 45.0% | |
| GTTGGTCCAAAGGACTCTAA+TGG | - | chr8.2:65528315-65528334 | MS.gene58303:CDS | 45.0% | |
| TATTCTCAATGGCGCGGTAT+CGG | - | chr8.2:65528119-65528138 | MS.gene58303:CDS | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.2:65528019-65528038 | MS.gene58303:CDS | 45.0% | |
| TGCAAGGTCAATCACGTTTG+AGG | - | chr8.2:65528035-65528054 | MS.gene58303:CDS | 45.0% | |
| TGGAATGGATGTTGGTCCAA+AGG | - | chr8.2:65528305-65528324 | MS.gene58303:CDS | 45.0% | |
| TTCGGATATTCTCAATGGCG+CGG | - | chr8.2:65528113-65528132 | MS.gene58303:CDS | 45.0% | |
| ! | GATGCATACGTTGTTGTTCG+AGG | - | chr8.2:65527901-65527920 | MS.gene58303:CDS | 45.0% |
| !! | GTGGCGGATGGAAAGAATTA+TGG | - | chr8.2:65528270-65528289 | MS.gene58303:CDS | 45.0% |
| AGGTATTCGACCTATTGGGG+TGG | - | chr8.2:65528077-65528096 | MS.gene58303:CDS | 50.0% | |
| GCAATTCTTGCCACCCCAAT+AGG | + | chr8.2:65528090-65528109 | None:intergenic | 50.0% | |
| TCGGTAACCGATCTTTGCTC+CGG | + | chr8.2:65528234-65528253 | None:intergenic | 50.0% | |
| TGGTGGTCGGTTAACTGGAA+TGG | - | chr8.2:65528290-65528309 | MS.gene58303:CDS | 50.0% | |
| GCAAAGATCGGTTACCGAGG+CGG | - | chr8.2:65528236-65528255 | MS.gene58303:CDS | 55.0% | |
| GGAGCAAAGATCGGTTACCG+AGG | - | chr8.2:65528233-65528252 | MS.gene58303:CDS | 55.0% | |
| GGTTGTGCCGGAGCAAAGAT+CGG | - | chr8.2:65528224-65528243 | MS.gene58303:CDS | 55.0% | |
| TACCGAGGCGGAGAAGCAAA+CGG | - | chr8.2:65528248-65528267 | MS.gene58303:CDS | 55.0% | |
| TAGTGTGAGGGTGGTTGTGC+CGG | - | chr8.2:65528212-65528231 | MS.gene58303:CDS | 55.0% | |
| CACCGTTTGCTTCTCCGCCT+CGG | + | chr8.2:65528253-65528272 | None:intergenic | 60.0% | |
| !! | GAGAAGCAAACGGTGGCGGA+TGG | - | chr8.2:65528258-65528277 | MS.gene58303:CDS | 60.0% |
| CGAGGCGGAGAAGCAAACGG+TGG | - | chr8.2:65528251-65528270 | MS.gene58303:CDS | 65.0% | |
| GGCGGAGAAGCAAACGGTGG+CGG | - | chr8.2:65528254-65528273 | MS.gene58303:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 65527835 | 65528386 | 65527835 | ID=MS.gene58303 |
| chr8.2 | mRNA | 65527835 | 65528386 | 65527835 | ID=MS.gene58303.t1;Parent=MS.gene58303 |
| chr8.2 | exon | 65527835 | 65528386 | 65527835 | ID=MS.gene58303.t1.exon1;Parent=MS.gene58303.t1 |
| chr8.2 | CDS | 65527835 | 65528386 | 65527835 | ID=cds.MS.gene58303.t1;Parent=MS.gene58303.t1 |
| Gene Sequence |
| Protein sequence |
