Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050410.t1 | AES82182.2 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 6.40E-71 | 276.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050410.t1 | Q32L65 | 36.1 | 72 | 34 | 2 | 10 | 71 | 44 | 113 | 4.2e-07 | 55.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050410.t1 | G7KST1 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 4.6e-71 | 276.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050409 | MS.gene050410 | 0.900655 | 5.90E-78 | -1.69E-46 |
| MS.gene050410 | MS.gene050412 | 0.905224 | 5.38E-80 | -1.69E-46 |
| MS.gene050410 | MS.gene065073 | 0.81375 | 2.18E-51 | -1.69E-46 |
| MS.gene050410 | MS.gene067785 | 0.805262 | 1.45E-49 | -1.69E-46 |
| MS.gene050410 | MS.gene07045 | 0.878537 | 2.59E-69 | -1.69E-46 |
| MS.gene050410 | MS.gene07046 | 0.906766 | 1.04E-80 | -1.69E-46 |
| MS.gene050410 | MS.gene07049 | 0.88672 | 2.67E-72 | -1.69E-46 |
| MS.gene050410 | MS.gene072704 | 0.807289 | 5.44E-50 | -1.69E-46 |
| MS.gene050410 | MS.gene36464 | 0.802365 | 5.81E-49 | -1.69E-46 |
| MS.gene050410 | MS.gene58303 | 0.800821 | 1.20E-48 | -1.69E-46 |
| MS.gene050410 | MS.gene61746 | 0.800563 | 1.36E-48 | -1.69E-46 |
| MS.gene050410 | MS.gene77183 | 0.806533 | 7.86E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050410.t1 | MTR_7g109460 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 7.77e-97 | 276 |
| MS.gene050410.t1 | MTR_7g109460 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 1.95e-96 | 276 |
| MS.gene050410.t1 | MTR_1g087150 | 64.138 | 145 | 43 | 2 | 1 | 136 | 1 | 145 | 3.56e-59 | 182 |
| MS.gene050410.t1 | MTR_1g087150 | 64.138 | 145 | 43 | 2 | 1 | 136 | 1 | 145 | 7.74e-59 | 182 |
| MS.gene050410.t1 | MTR_1g108490 | 42.063 | 126 | 63 | 3 | 17 | 135 | 56 | 178 | 4.58e-29 | 105 |
| MS.gene050410.t1 | MTR_1g108490 | 42.063 | 126 | 63 | 3 | 17 | 135 | 56 | 178 | 1.16e-28 | 105 |
| MS.gene050410.t1 | MTR_8g467220 | 58.904 | 73 | 28 | 1 | 9 | 81 | 56 | 126 | 2.58e-25 | 97.4 |
| MS.gene050410.t1 | MTR_5g040650 | 42.623 | 122 | 58 | 4 | 20 | 132 | 70 | 188 | 5.68e-25 | 95.9 |
| MS.gene050410.t1 | MTR_5g040650 | 42.623 | 122 | 58 | 4 | 20 | 132 | 70 | 188 | 1.08e-24 | 95.9 |
| MS.gene050410.t1 | MTR_7g082950 | 64.516 | 62 | 19 | 2 | 19 | 80 | 15 | 73 | 7.11e-24 | 90.9 |
| MS.gene050410.t1 | MTR_7g082950 | 64.516 | 62 | 19 | 2 | 19 | 80 | 15 | 73 | 1.01e-23 | 91.7 |
| MS.gene050410.t1 | MTR_7g010610 | 38.318 | 107 | 57 | 4 | 1 | 105 | 16 | 115 | 5.82e-18 | 75.9 |
| MS.gene050410.t1 | MTR_3g053370 | 51.613 | 62 | 6 | 1 | 32 | 93 | 3 | 40 | 1.55e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050410.t1 | AT1G02610 | 52.518 | 139 | 59 | 3 | 1 | 135 | 1 | 136 | 3.46e-40 | 133 |
| MS.gene050410.t1 | AT1G02610 | 52.518 | 139 | 59 | 3 | 1 | 135 | 1 | 136 | 5.47e-40 | 133 |
| MS.gene050410.t1 | AT4G02075 | 50.725 | 138 | 58 | 4 | 1 | 136 | 1 | 130 | 4.95e-36 | 122 |
| MS.gene050410.t1 | AT4G02075 | 50.725 | 138 | 58 | 4 | 1 | 136 | 1 | 130 | 3.77e-35 | 120 |
| MS.gene050410.t1 | AT4G02075 | 50.725 | 138 | 58 | 4 | 1 | 136 | 1 | 130 | 4.20e-35 | 121 |
| MS.gene050410.t1 | AT2G01275 | 46.774 | 124 | 56 | 5 | 16 | 135 | 81 | 198 | 2.46e-30 | 110 |
| MS.gene050410.t1 | AT2G01275 | 46.774 | 124 | 56 | 5 | 16 | 135 | 81 | 198 | 2.46e-30 | 110 |
| MS.gene050410.t1 | AT2G01275 | 46.774 | 124 | 56 | 5 | 16 | 135 | 55 | 172 | 2.56e-30 | 110 |
| MS.gene050410.t1 | AT2G01275 | 46.774 | 124 | 56 | 5 | 16 | 135 | 55 | 172 | 2.56e-30 | 110 |
| MS.gene050410.t1 | AT2G01275 | 46.774 | 124 | 56 | 5 | 16 | 135 | 55 | 172 | 2.56e-30 | 110 |
| MS.gene050410.t1 | AT5G38070 | 64.062 | 64 | 21 | 1 | 15 | 78 | 49 | 110 | 2.53e-26 | 99.8 |
| MS.gene050410.t1 | AT2G02960 | 44.167 | 120 | 57 | 3 | 20 | 132 | 43 | 159 | 6.47e-26 | 99.0 |
| MS.gene050410.t1 | AT2G02960 | 44.167 | 120 | 57 | 3 | 20 | 132 | 43 | 159 | 6.47e-26 | 99.0 |
| MS.gene050410.t1 | AT2G02960 | 44.167 | 120 | 57 | 3 | 20 | 132 | 43 | 159 | 6.47e-26 | 99.0 |
| MS.gene050410.t1 | AT2G02960 | 44.167 | 120 | 57 | 3 | 20 | 132 | 43 | 159 | 6.47e-26 | 99.0 |
| MS.gene050410.t1 | AT2G02960 | 44.167 | 120 | 57 | 3 | 20 | 132 | 43 | 159 | 6.47e-26 | 99.0 |
| MS.gene050410.t1 | AT2G02960 | 44.167 | 120 | 57 | 3 | 20 | 132 | 43 | 159 | 2.18e-25 | 97.4 |
| MS.gene050410.t1 | AT3G47550 | 42.623 | 122 | 56 | 3 | 20 | 132 | 69 | 185 | 4.36e-24 | 93.6 |
| MS.gene050410.t1 | AT3G47550 | 45.455 | 121 | 54 | 4 | 20 | 132 | 69 | 185 | 6.18e-24 | 92.8 |
| MS.gene050410.t1 | AT3G47550 | 42.623 | 122 | 56 | 3 | 20 | 132 | 69 | 185 | 1.28e-23 | 93.2 |
| MS.gene050410.t1 | AT3G47550 | 42.623 | 122 | 56 | 3 | 20 | 132 | 69 | 185 | 1.28e-23 | 93.2 |
| MS.gene050410.t1 | AT5G62460 | 69.643 | 56 | 15 | 1 | 20 | 75 | 78 | 131 | 2.86e-23 | 92.4 |
| MS.gene050410.t1 | AT5G62460 | 69.643 | 56 | 15 | 1 | 20 | 75 | 78 | 131 | 2.86e-23 | 92.4 |
| MS.gene050410.t1 | AT5G62460 | 69.643 | 56 | 15 | 1 | 20 | 75 | 91 | 144 | 2.92e-23 | 92.8 |
| MS.gene050410.t1 | AT1G14260 | 41.935 | 124 | 58 | 4 | 20 | 134 | 57 | 175 | 8.92e-21 | 85.1 |
| MS.gene050410.t1 | AT1G14260 | 41.935 | 124 | 58 | 4 | 20 | 134 | 57 | 175 | 8.92e-21 | 85.1 |
| MS.gene050410.t1 | AT1G14260 | 41.935 | 124 | 58 | 4 | 20 | 134 | 57 | 175 | 8.92e-21 | 85.1 |
| MS.gene050410.t1 | AT4G32670 | 38.462 | 65 | 37 | 2 | 20 | 82 | 33 | 96 | 3.27e-11 | 60.1 |
| MS.gene050410.t1 | AT4G32670 | 38.462 | 65 | 37 | 2 | 20 | 82 | 33 | 96 | 3.27e-11 | 60.1 |
Find 24 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTACTAATCGATGATTTA+AGG | 0.215992 | 7.1:+4835620 | MS.gene050410:CDS |
| GAAGCACCTTGTTCTTGTTC+TGG | 0.299149 | 7.1:+4835709 | MS.gene050410:CDS |
| CGCGTTTCACTGTCGATCAT+TGG | 0.320595 | 7.1:+4836923 | MS.gene050410:CDS |
| ACAAGAACAAGGTGCTTCTA+AGG | 0.336965 | 7.1:-4835704 | None:intergenic |
| ACAAGGTGCTTCTAAGGCTT+TGG | 0.357018 | 7.1:-4835698 | None:intergenic |
| AGCTTTCAAACTCTTCTTCA+TGG | 0.379338 | 7.1:-4835675 | None:intergenic |
| TTCTTGTTCTGGAACTGTTA+AGG | 0.379888 | 7.1:+4835720 | MS.gene050410:CDS |
| TAGTGGTAGAGCTGCATTCC+GGG | 0.387977 | 7.1:-4836893 | None:intergenic |
| TTAGTGGTAGAGCTGCATTC+CGG | 0.388354 | 7.1:-4836894 | None:intergenic |
| TGGAGGAGGAGCTGTATATC+CGG | 0.422547 | 7.1:-4836509 | None:intergenic |
| AACGCGGTAAAGTACCTTAT+GGG | 0.451704 | 7.1:-4836562 | None:intergenic |
| ATGATGAAGCAATGCCCATA+AGG | 0.464358 | 7.1:+4836548 | MS.gene050410:CDS |
| GTGAAACGCGGTTCTGTTAG+TGG | 0.469197 | 7.1:-4836910 | None:intergenic |
| CAAAGATGGTGTAACGAGAA+AGG | 0.507130 | 7.1:+4835902 | MS.gene050410:CDS |
| GATGATTTAAGGTTACTCTC+TGG | 0.510571 | 7.1:+4835631 | MS.gene050410:CDS |
| GGAGGAGGAGCTGTATATCC+GGG | 0.519973 | 7.1:-4836508 | None:intergenic |
| ATAGAGATTGCATACAAAGA+TGG | 0.547272 | 7.1:+4835888 | MS.gene050410:CDS |
| AACATGTGAAATATGTCTCC+AGG | 0.548388 | 7.1:+4835931 | MS.gene050410:CDS |
| TGTAATAGAAAGTGATTACC+CGG | 0.600485 | 7.1:+4836875 | MS.gene050410:CDS |
| ATAAGAGAAAGTGAGGTGGA+AGG | 0.645126 | 7.1:+4836849 | MS.gene050410:CDS |
| AAACGCGATAAGAGAAAGTG+AGG | 0.650705 | 7.1:+4836842 | MS.gene050410:CDS |
| CGCGATAAGAGAAAGTGAGG+TGG | 0.650995 | 7.1:+4836845 | MS.gene050410:CDS |
| CAATGATCGACAGTGAAACG+CGG | 0.703113 | 7.1:-4836922 | None:intergenic |
| ACAGTTCCAGAACAAGAACA+AGG | 0.719305 | 7.1:-4835715 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAATAACACATTTAAT+TGG | + | chr7.1:4836749-4836768 | MS.gene050410:intron | 10.0% |
| !! | AATAGAAAGAAAAAATAAAG+AGG | + | chr7.1:4836080-4836099 | MS.gene050410:intron | 15.0% |
| !! | ATAGAAAGAAAAAATAAAGA+GGG | + | chr7.1:4836081-4836100 | MS.gene050410:intron | 15.0% |
| !! | ATATAAGTCAAAATTAGATT+GGG | - | chr7.1:4836181-4836200 | None:intergenic | 15.0% |
| !! | CTACAAATTATAATATCAAA+TGG | - | chr7.1:4836352-4836371 | None:intergenic | 15.0% |
| !! | TATATAAGTCAAAATTAGAT+TGG | - | chr7.1:4836182-4836201 | None:intergenic | 15.0% |
| !!! | AAATTTGATAACACATTTTA+AGG | + | chr7.1:4836802-4836821 | MS.gene050410:intron | 15.0% |
| !!! | AATTTGATAACACATTTTAA+GGG | + | chr7.1:4836803-4836822 | MS.gene050410:intron | 15.0% |
| !!! | CATTTGATATTATAATTTGT+AGG | + | chr7.1:4836350-4836369 | MS.gene050410:intron | 15.0% |
| !! | CATGATAAATATAACAAGAT+GGG | + | chr7.1:4836435-4836454 | MS.gene050410:intron | 20.0% |
| !!! | ATTTGATAACACATTTTAAG+GGG | + | chr7.1:4836804-4836823 | MS.gene050410:intron | 20.0% |
| !!! | TATAATTTGTAGGAATTATC+AGG | + | chr7.1:4836360-4836379 | MS.gene050410:intron | 20.0% |
| !!! | TCATTTATCTCTGATTTTTT+TGG | - | chr7.1:4836532-4836551 | None:intergenic | 20.0% |
| ! | AATTATGTTGTGACGTAAAT+TGG | + | chr7.1:4836147-4836166 | MS.gene050410:intron | 25.0% |
| ! | GAAATGAAGATAGAACTAAA+GGG | - | chr7.1:4836330-4836349 | None:intergenic | 25.0% |
| ! | GCATGATAAATATAACAAGA+TGG | + | chr7.1:4836434-4836453 | MS.gene050410:intron | 25.0% |
| ! | TGTTACTAATCGATGATTTA+AGG | + | chr7.1:4835620-4835639 | MS.gene050410:CDS | 25.0% |
| ! | TTAAATTTCTCAGCTAATCA+TGG | + | chr7.1:4836304-4836323 | MS.gene050410:intron | 25.0% |
| ! | TTATGTTCATTGTATGCATA+AGG | - | chr7.1:4836396-4836415 | None:intergenic | 25.0% |
| !!! | CTTTTTGATTGAATTAAACG+CGG | - | chr7.1:4836581-4836600 | None:intergenic | 25.0% |
| !!! | TTTATCTCTGATTTTTTTGG+AGG | - | chr7.1:4836529-4836548 | None:intergenic | 25.0% |
| !!! | TTTGATAACACATTTTAAGG+GGG | + | chr7.1:4836805-4836824 | MS.gene050410:intron | 25.0% |
| ATAGAGATTGCATACAAAGA+TGG | + | chr7.1:4835888-4835907 | MS.gene050410:CDS | 30.0% | |
| GGAAATGAAGATAGAACTAA+AGG | - | chr7.1:4836331-4836350 | None:intergenic | 30.0% | |
| TATTAAGTCTTAGCTACACA+TGG | + | chr7.1:4836639-4836658 | MS.gene050410:intron | 30.0% | |
| TGTAATAGAAAGTGATTACC+CGG | + | chr7.1:4836875-4836894 | MS.gene050410:CDS | 30.0% | |
| !! | TTGATAACACATTTTAAGGG+GGG | + | chr7.1:4836806-4836825 | MS.gene050410:intron | 30.0% |
| AACATGTGAAATATGTCTCC+AGG | + | chr7.1:4835931-4835950 | MS.gene050410:CDS | 35.0% | |
| AGCTTTCAAACTCTTCTTCA+TGG | - | chr7.1:4835678-4835697 | None:intergenic | 35.0% | |
| ATGTTGTGACGTAAATTGGA+TGG | + | chr7.1:4836151-4836170 | MS.gene050410:intron | 35.0% | |
| TAATCATCATCACACATACC+TGG | - | chr7.1:4835952-4835971 | None:intergenic | 35.0% | |
| TGTTGTGACGTAAATTGGAT+GGG | + | chr7.1:4836152-4836171 | MS.gene050410:intron | 35.0% | |
| ! | GATGATTTAAGGTTACTCTC+TGG | + | chr7.1:4835631-4835650 | MS.gene050410:CDS | 35.0% |
| ! | GGTGCAACTATTTTATACGT+TGG | + | chr7.1:4836668-4836687 | MS.gene050410:intron | 35.0% |
| ! | TTTTTGCAGCAATATGAACC+CGG | + | chr7.1:4836490-4836509 | MS.gene050410:intron | 35.0% |
| !! | TTCTTGTTCTGGAACTGTTA+AGG | + | chr7.1:4835720-4835739 | MS.gene050410:CDS | 35.0% |
| !!! | ATCTCTGATTTTTTTGGAGG+AGG | - | chr7.1:4836526-4836545 | None:intergenic | 35.0% |
| AAACGCGATAAGAGAAAGTG+AGG | + | chr7.1:4836842-4836861 | MS.gene050410:CDS | 40.0% | |
| ACAGTTCCAGAACAAGAACA+AGG | - | chr7.1:4835718-4835737 | None:intergenic | 40.0% | |
| ATAAGAGAAAGTGAGGTGGA+AGG | + | chr7.1:4836849-4836868 | MS.gene050410:CDS | 40.0% | |
| ATGATGAAGCAATGCCCATA+AGG | + | chr7.1:4836548-4836567 | MS.gene050410:CDS | 40.0% | |
| CAAAGATGGTGTAACGAGAA+AGG | + | chr7.1:4835902-4835921 | MS.gene050410:CDS | 40.0% | |
| ! | AAACGCGGTAAAGTACCTTA+TGG | - | chr7.1:4836566-4836585 | None:intergenic | 40.0% |
| ! | AACGCGGTAAAGTACCTTAT+GGG | - | chr7.1:4836565-4836584 | None:intergenic | 40.0% |
| ! | ACAAGAACAAGGTGCTTCTA+AGG | - | chr7.1:4835707-4835726 | None:intergenic | 40.0% |
| !! | ACACGTATTTTGCTGTCGTT+AGG | + | chr7.1:4836124-4836143 | MS.gene050410:intron | 40.0% |
| AGTCTTAGCTACACATGGCA+TGG | + | chr7.1:4836644-4836663 | MS.gene050410:intron | 45.0% | |
| CAATGATCGACAGTGAAACG+CGG | - | chr7.1:4836925-4836944 | None:intergenic | 45.0% | |
| GGGAGAAAGAGTTATCTGTG+AGG | + | chr7.1:4836101-4836120 | MS.gene050410:intron | 45.0% | |
| TTAGTGGTAGAGCTGCATTC+CGG | - | chr7.1:4836897-4836916 | None:intergenic | 45.0% | |
| ! | GAAGCACCTTGTTCTTGTTC+TGG | + | chr7.1:4835709-4835728 | MS.gene050410:CDS | 45.0% |
| !! | ACAAGGTGCTTCTAAGGCTT+TGG | - | chr7.1:4835701-4835720 | None:intergenic | 45.0% |
| CGCGATAAGAGAAAGTGAGG+TGG | + | chr7.1:4836845-4836864 | MS.gene050410:CDS | 50.0% | |
| CGCGTTTCACTGTCGATCAT+TGG | + | chr7.1:4836923-4836942 | MS.gene050410:CDS | 50.0% | |
| CTTAGCTACACATGGCATGG+AGG | + | chr7.1:4836647-4836666 | MS.gene050410:intron | 50.0% | |
| GTGAAACGCGGTTCTGTTAG+TGG | - | chr7.1:4836913-4836932 | None:intergenic | 50.0% | |
| TAGTGGTAGAGCTGCATTCC+GGG | - | chr7.1:4836896-4836915 | None:intergenic | 50.0% | |
| TGGAGGAGGAGCTGTATATC+CGG | - | chr7.1:4836512-4836531 | None:intergenic | 50.0% | |
| GGAGGAGGAGCTGTATATCC+GGG | - | chr7.1:4836511-4836530 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 4835607 | 4836953 | 4835607 | ID=MS.gene050410 |
| chr7.1 | mRNA | 4835607 | 4836953 | 4835607 | ID=MS.gene050410.t1;Parent=MS.gene050410 |
| chr7.1 | exon | 4835607 | 4835741 | 4835607 | ID=MS.gene050410.t1.exon1;Parent=MS.gene050410.t1 |
| chr7.1 | CDS | 4835607 | 4835741 | 4835607 | ID=cds.MS.gene050410.t1;Parent=MS.gene050410.t1 |
| chr7.1 | exon | 4835881 | 4835952 | 4835881 | ID=MS.gene050410.t1.exon2;Parent=MS.gene050410.t1 |
| chr7.1 | CDS | 4835881 | 4835952 | 4835881 | ID=cds.MS.gene050410.t1;Parent=MS.gene050410.t1 |
| chr7.1 | exon | 4836499 | 4836569 | 4836499 | ID=MS.gene050410.t1.exon3;Parent=MS.gene050410.t1 |
| chr7.1 | CDS | 4836499 | 4836569 | 4836499 | ID=cds.MS.gene050410.t1;Parent=MS.gene050410.t1 |
| chr7.1 | exon | 4836824 | 4836953 | 4836824 | ID=MS.gene050410.t1.exon4;Parent=MS.gene050410.t1 |
| chr7.1 | CDS | 4836824 | 4836953 | 4836824 | ID=cds.MS.gene050410.t1;Parent=MS.gene050410.t1 |
| Gene Sequence |
| Protein sequence |
