Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58305.t1 | XP_013444909.1 | 87.1 | 178 | 22 | 1 | 1 | 177 | 1 | 178 | 1.40E-81 | 312.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58305.t1 | Q58FX0 | 34.2 | 202 | 100 | 6 | 3 | 175 | 8 | 205 | 2.7e-22 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58305.t1 | I3SZG8 | 87.1 | 178 | 22 | 1 | 1 | 177 | 1 | 178 | 9.8e-82 | 312.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051837 | MS.gene58305 | 0.810788 | 9.68E-51 | -1.69E-46 |
| MS.gene06061 | MS.gene58305 | 0.842205 | 2.98E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58305.t1 | MTR_8g432370 | 87.079 | 178 | 22 | 1 | 1 | 177 | 1 | 178 | 1.16e-110 | 313 |
| MS.gene58305.t1 | MTR_8g432390 | 88.268 | 179 | 19 | 2 | 1 | 177 | 1 | 179 | 1.83e-110 | 312 |
| MS.gene58305.t1 | MTR_8g432350 | 86.592 | 179 | 22 | 2 | 1 | 177 | 1 | 179 | 5.41e-109 | 308 |
| MS.gene58305.t1 | MTR_8g432400 | 88.827 | 179 | 18 | 2 | 1 | 177 | 1 | 179 | 1.53e-108 | 307 |
| MS.gene58305.t1 | MTR_7g076000 | 53.039 | 181 | 65 | 6 | 1 | 176 | 1 | 166 | 5.65e-54 | 169 |
| MS.gene58305.t1 | MTR_8g432440 | 36.612 | 183 | 98 | 5 | 2 | 173 | 5 | 180 | 2.73e-28 | 104 |
| MS.gene58305.t1 | MTR_8g432420 | 36.612 | 183 | 98 | 5 | 2 | 173 | 5 | 180 | 2.73e-28 | 104 |
| MS.gene58305.t1 | MTR_8g432470 | 37.158 | 183 | 97 | 5 | 2 | 173 | 5 | 180 | 7.74e-28 | 103 |
| MS.gene58305.t1 | MTR_8g432450 | 35.593 | 177 | 98 | 3 | 2 | 167 | 5 | 176 | 2.15e-26 | 99.4 |
| MS.gene58305.t1 | MTR_8g432460 | 35.165 | 182 | 101 | 5 | 2 | 173 | 5 | 179 | 6.43e-24 | 92.8 |
| MS.gene58305.t1 | MTR_8g432490 | 35.165 | 182 | 101 | 5 | 2 | 173 | 5 | 179 | 6.26e-23 | 90.5 |
| MS.gene58305.t1 | MTR_3g064780 | 34.066 | 182 | 103 | 5 | 2 | 173 | 5 | 179 | 1.28e-21 | 88.6 |
| MS.gene58305.t1 | MTR_8g432480 | 32.727 | 165 | 98 | 3 | 2 | 155 | 7 | 169 | 1.30e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58305.t1 | AT2G45760 | 36.585 | 205 | 95 | 8 | 1 | 175 | 6 | 205 | 1.06e-32 | 116 |
| MS.gene58305.t1 | AT3G61190 | 37.912 | 182 | 103 | 6 | 1 | 177 | 16 | 192 | 3.29e-29 | 107 |
| MS.gene58305.t1 | AT3G61190 | 37.912 | 182 | 103 | 6 | 1 | 177 | 16 | 192 | 3.29e-29 | 107 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACAACAAAGCCATAAA+AGG | 0.230681 | 8.2:-65548939 | MS.gene58305:CDS |
| CACCCTTTCAAATGTGTCTT+TGG | 0.267837 | 8.2:+65548469 | None:intergenic |
| CAACAGGGATTCCTGCTGTT+TGG | 0.341709 | 8.2:-65548500 | MS.gene58305:CDS |
| ATAATAGAGTTAGGAGAAAC+GGG | 0.341844 | 8.2:-65548650 | MS.gene58305:CDS |
| GGTGTTCCGGTGGCCGGTAA+TGG | 0.375336 | 8.2:-65548540 | MS.gene58305:CDS |
| TCCTGCAGCAGAGTTGCAAC+AGG | 0.395408 | 8.2:-65548516 | MS.gene58305:CDS |
| ATTAGTGGAGTTCCGGTAAC+TGG | 0.400709 | 8.2:-65548561 | MS.gene58305:CDS |
| AATTAGAACAAGAACTTTGT+TGG | 0.403293 | 8.2:+65548595 | None:intergenic |
| ATTAGAACAAGAACTTTGTT+GGG | 0.411878 | 8.2:+65548596 | None:intergenic |
| GTCATCGATTAGTGGAGTTC+CGG | 0.435279 | 8.2:-65548568 | MS.gene58305:CDS |
| GACATACCCTCCAAGAAAAT+CGG | 0.435714 | 8.2:+65548713 | None:intergenic |
| CCCTGTTGCAACTCTGCTGC+AGG | 0.438745 | 8.2:+65548515 | None:intergenic |
| TCTGCTGCAGGAGCCATTAC+CGG | 0.444058 | 8.2:+65548527 | None:intergenic |
| AGTTCCGGTAACTGGTGTTC+CGG | 0.450510 | 8.2:-65548553 | MS.gene58305:CDS |
| AATTCGATGTCATCGATTAG+TGG | 0.458468 | 8.2:-65548576 | MS.gene58305:CDS |
| AGTAGGGTAACTTCCGCCTT+CGG | 0.464742 | 8.2:+65548851 | None:intergenic |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8.2:-65548659 | MS.gene58305:CDS |
| CCAACTTCTCGTTCCAAGTA+GGG | 0.480749 | 8.2:+65548835 | None:intergenic |
| AAGTAGTAGCAGTGTTGGTA+TGG | 0.480912 | 8.2:-65548751 | MS.gene58305:CDS |
| AAGGCGGAAGTTACCCTACT+TGG | 0.483567 | 8.2:-65548848 | MS.gene58305:CDS |
| TGGTAGTTTAACCAAACAGC+AGG | 0.487555 | 8.2:+65548489 | None:intergenic |
| GCCTTCGGAATCAAGTTTCG+TGG | 0.489360 | 8.2:+65548866 | None:intergenic |
| GTTATGAACCTTGCATGTAA+TGG | 0.492338 | 8.2:+65548801 | None:intergenic |
| AGAACAACAAAGCCATAAAA+GGG | 0.500399 | 8.2:-65548938 | MS.gene58305:CDS |
| GCCACCGGAACACCAGTTAC+CGG | 0.504701 | 8.2:+65548549 | None:intergenic |
| GATAATAGAGTTAGGAGAAA+CGG | 0.505051 | 8.2:-65548651 | MS.gene58305:CDS |
| GTAACTGGTGTTCCGGTGGC+CGG | 0.509562 | 8.2:-65548546 | MS.gene58305:CDS |
| TGTTGGTATGGCTAGAATAC+CGG | 0.512707 | 8.2:-65548739 | MS.gene58305:CDS |
| AGAGGAAGTAGTAGCAGTGT+TGG | 0.515573 | 8.2:-65548756 | MS.gene58305:CDS |
| CCTCCAAGAAAATCGGAAAC+CGG | 0.520232 | 8.2:+65548720 | None:intergenic |
| TGCAAGGTTCATAACAATAG+AGG | 0.521084 | 8.2:-65548793 | MS.gene58305:CDS |
| TTGGAACGAGAAGTTGGTAA+TGG | 0.534411 | 8.2:-65548829 | MS.gene58305:CDS |
| CAGGAGCCATTACCGGCCAC+CGG | 0.541843 | 8.2:+65548534 | None:intergenic |
| CCCTACTTGGAACGAGAAGT+TGG | 0.543637 | 8.2:-65548835 | MS.gene58305:CDS |
| TCCGGTAACTGGTGTTCCGG+TGG | 0.577500 | 8.2:-65548550 | MS.gene58305:CDS |
| GGTTGTAAATATTTCAGTGA+AGG | 0.577908 | 8.2:-65548628 | MS.gene58305:CDS |
| ACCAACTTCTCGTTCCAAGT+AGG | 0.585914 | 8.2:+65548834 | None:intergenic |
| TTTGTCACGGTTCAATGTGA+CGG | 0.605787 | 8.2:-65548909 | MS.gene58305:CDS |
| TGGATGTGCCATTACATGCA+AGG | 0.624126 | 8.2:-65548809 | MS.gene58305:CDS |
| GAGGTGAAGTACAAAACTAG+AGG | 0.629180 | 8.2:-65548774 | MS.gene58305:CDS |
| ACGAAACTTGATTCCGAAGG+CGG | 0.631770 | 8.2:-65548864 | MS.gene58305:CDS |
| AAAAGGGAACACATTTGTCA+CGG | 0.633602 | 8.2:-65548922 | MS.gene58305:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.642396 | 8.2:-65548867 | MS.gene58305:CDS |
| CCTGCAGCAGAGTTGCAACA+GGG | 0.674047 | 8.2:-65548515 | MS.gene58305:CDS |
| TAATAGAGTTAGGAGAAACG+GGG | 0.700815 | 8.2:-65548649 | MS.gene58305:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | - | chr8.2:65548783-65548802 | MS.gene58305:CDS | 15.0% |
| !!! | AAAATTGCAATTGATTTTCA+TGG | + | chr8.2:65548775-65548794 | None:intergenic | 20.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | - | chr8.2:65548789-65548808 | MS.gene58305:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | - | chr8.2:65548790-65548809 | MS.gene58305:CDS | 20.0% |
| ! | AATTAGAACAAGAACTTTGT+TGG | + | chr8.2:65548871-65548890 | None:intergenic | 25.0% |
| ! | ATTAGAACAAGAACTTTGTT+GGG | + | chr8.2:65548870-65548889 | None:intergenic | 25.0% |
| AGAACAACAAAGCCATAAAA+GGG | - | chr8.2:65548525-65548544 | MS.gene58305:CDS | 30.0% | |
| ATAATAGAGTTAGGAGAAAC+GGG | - | chr8.2:65548813-65548832 | MS.gene58305:CDS | 30.0% | |
| GATAATAGAGTTAGGAGAAA+CGG | - | chr8.2:65548812-65548831 | MS.gene58305:CDS | 30.0% | |
| GGTTGTAAATATTTCAGTGA+AGG | - | chr8.2:65548835-65548854 | MS.gene58305:CDS | 30.0% | |
| AAAAGGGAACACATTTGTCA+CGG | - | chr8.2:65548541-65548560 | MS.gene58305:CDS | 35.0% | |
| GAGAACAACAAAGCCATAAA+AGG | - | chr8.2:65548524-65548543 | MS.gene58305:CDS | 35.0% | |
| GTTATGAACCTTGCATGTAA+TGG | + | chr8.2:65548665-65548684 | None:intergenic | 35.0% | |
| TAATAGAGTTAGGAGAAACG+GGG | - | chr8.2:65548814-65548833 | MS.gene58305:CDS | 35.0% | |
| TGCAAGGTTCATAACAATAG+AGG | - | chr8.2:65548670-65548689 | MS.gene58305:CDS | 35.0% | |
| ! | AATTCGATGTCATCGATTAG+TGG | - | chr8.2:65548887-65548906 | MS.gene58305:CDS | 35.0% |
| ! | AGAGATGACTATTTTGTCAG+CGG | - | chr8.2:65548490-65548509 | MS.gene58305:CDS | 35.0% |
| ! | GACAAATGTGTTCCCTTTTA+TGG | + | chr8.2:65548540-65548559 | None:intergenic | 35.0% |
| GACATACCCTCCAAGAAAAT+CGG | + | chr8.2:65548753-65548772 | None:intergenic | 40.0% | |
| GAGGTGAAGTACAAAACTAG+AGG | - | chr8.2:65548689-65548708 | MS.gene58305:CDS | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | - | chr8.2:65548804-65548823 | MS.gene58305:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+AGG | + | chr8.2:65548977-65548996 | None:intergenic | 40.0% | |
| TGTTGGTATGGCTAGAATAC+CGG | - | chr8.2:65548724-65548743 | MS.gene58305:CDS | 40.0% | |
| TTTGTCACGGTTCAATGTGA+CGG | - | chr8.2:65548554-65548573 | MS.gene58305:CDS | 40.0% | |
| !! | AAGTAGTAGCAGTGTTGGTA+TGG | - | chr8.2:65548712-65548731 | MS.gene58305:CDS | 40.0% |
| !! | TTGGAACGAGAAGTTGGTAA+TGG | - | chr8.2:65548634-65548653 | MS.gene58305:CDS | 40.0% |
| !!! | ATACCGGTTTCCGATTTTCT+TGG | - | chr8.2:65548740-65548759 | MS.gene58305:CDS | 40.0% |
| ACCAACTTCTCGTTCCAAGT+AGG | + | chr8.2:65548632-65548651 | None:intergenic | 45.0% | |
| ACCACGAAACTTGATTCCGA+AGG | - | chr8.2:65548596-65548615 | MS.gene58305:CDS | 45.0% | |
| ACGAAACTTGATTCCGAAGG+CGG | - | chr8.2:65548599-65548618 | MS.gene58305:CDS | 45.0% | |
| AGAGGAAGTAGTAGCAGTGT+TGG | - | chr8.2:65548707-65548726 | MS.gene58305:CDS | 45.0% | |
| ATTAGTGGAGTTCCGGTAAC+TGG | - | chr8.2:65548902-65548921 | MS.gene58305:CDS | 45.0% | |
| CCAACTTCTCGTTCCAAGTA+GGG | + | chr8.2:65548631-65548650 | None:intergenic | 45.0% | |
| CCTCCAAGAAAATCGGAAAC+CGG | + | chr8.2:65548746-65548765 | None:intergenic | 45.0% | |
| ! | CGGTTTCCGATTTTCTTGGA+GGG | - | chr8.2:65548744-65548763 | MS.gene58305:CDS | 45.0% |
| ! | GTCATCGATTAGTGGAGTTC+CGG | - | chr8.2:65548895-65548914 | MS.gene58305:CDS | 45.0% |
| ! | TGGATGTGCCATTACATGCA+AGG | - | chr8.2:65548654-65548673 | MS.gene58305:CDS | 45.0% |
| AAGGCGGAAGTTACCCTACT+TGG | - | chr8.2:65548615-65548634 | MS.gene58305:CDS | 50.0% | |
| AGTAGGGTAACTTCCGCCTT+CGG | + | chr8.2:65548615-65548634 | None:intergenic | 50.0% | |
| AGTTCCGGTAACTGGTGTTC+CGG | - | chr8.2:65548910-65548929 | MS.gene58305:CDS | 50.0% | |
| CAACAGGGATTCCTGCTGTT+TGG | - | chr8.2:65548963-65548982 | MS.gene58305:CDS | 50.0% | |
| CCCTACTTGGAACGAGAAGT+TGG | - | chr8.2:65548628-65548647 | MS.gene58305:CDS | 50.0% | |
| GCCTTCGGAATCAAGTTTCG+TGG | + | chr8.2:65548600-65548619 | None:intergenic | 50.0% | |
| ! | CCGGTTTCCGATTTTCTTGG+AGG | - | chr8.2:65548743-65548762 | MS.gene58305:CDS | 50.0% |
| CCTGCAGCAGAGTTGCAACA+GGG | - | chr8.2:65548948-65548967 | MS.gene58305:CDS | 55.0% | |
| TCCTGCAGCAGAGTTGCAAC+AGG | - | chr8.2:65548947-65548966 | MS.gene58305:CDS | 55.0% | |
| TCTGCTGCAGGAGCCATTAC+CGG | + | chr8.2:65548939-65548958 | None:intergenic | 55.0% | |
| CCCTGTTGCAACTCTGCTGC+AGG | + | chr8.2:65548951-65548970 | None:intergenic | 60.0% | |
| GCCACCGGAACACCAGTTAC+CGG | + | chr8.2:65548917-65548936 | None:intergenic | 60.0% | |
| GTAACTGGTGTTCCGGTGGC+CGG | - | chr8.2:65548917-65548936 | MS.gene58305:CDS | 60.0% | |
| TCCGGTAACTGGTGTTCCGG+TGG | - | chr8.2:65548913-65548932 | MS.gene58305:CDS | 60.0% | |
| CAGGAGCCATTACCGGCCAC+CGG | + | chr8.2:65548932-65548951 | None:intergenic | 65.0% | |
| GGTGTTCCGGTGGCCGGTAA+TGG | - | chr8.2:65548923-65548942 | MS.gene58305:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 65548476 | 65549009 | 65548476 | ID=MS.gene58305 |
| chr8.2 | mRNA | 65548476 | 65549009 | 65548476 | ID=MS.gene58305.t1;Parent=MS.gene58305 |
| chr8.2 | exon | 65548476 | 65549009 | 65548476 | ID=MS.gene58305.t1.exon1;Parent=MS.gene58305.t1 |
| chr8.2 | CDS | 65548476 | 65549009 | 65548476 | ID=cds.MS.gene58305.t1;Parent=MS.gene58305.t1 |
| Gene Sequence |
| Protein sequence |
