Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58355.t1 | XP_013464663.1 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.40E-50 | 208.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58355.t1 | A0A072VB72 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.0e-50 | 208.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05474 | MS.gene58355 | 0.808205 | 3.47E-50 | -1.69E-46 |
| MS.gene059776 | MS.gene58355 | 0.998467 | 6.27E-266 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58355.t1 | MTR_2g078780 | 92.437 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.50e-76 | 222 |
| MS.gene58355.t1 | MTR_1g061895 | 93.939 | 99 | 6 | 0 | 1 | 99 | 1 | 99 | 4.04e-63 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTGAAAGAAAAAAAAATTG+AGG | - | 14450:6348-6367 | MS.gene58355:intergenic | 20.0% |
| !!! | TTTTTTTTTTAAATTCAGGG+TGG | + | 14450:6432-6451 | MS.gene58355:intron | 20.0% |
| ! | AACAAAGAAAACTGTACAAT+TGG | + | 14450:6557-6576 | MS.gene58355:CDS | 25.0% |
| AGAAGTAGTGAAGATTTATC+AGG | + | 14450:6636-6655 | MS.gene58355:CDS | 30.0% | |
| CATTAGGAGCAATAAATCAA+GGG | + | 14450:6460-6479 | MS.gene58355:CDS | 30.0% | |
| GAAGTAGTGAAGATTTATCA+GGG | + | 14450:6637-6656 | MS.gene58355:CDS | 30.0% | |
| CCATTAGGAGCAATAAATCA+AGG | + | 14450:6459-6478 | MS.gene58355:CDS | 35.0% | |
| CCTTGATTTATTGCTCCTAA+TGG | - | 14450:6462-6481 | MS.gene58355:intergenic | 35.0% | |
| !! | AGAAGTTGGTAAGGAAAATG+TGG | + | 14450:6518-6537 | MS.gene58355:CDS | 35.0% |
| !! | GTTGATAACTTTAGCAGCAT+TGG | - | 14450:6284-6303 | MS.gene58355:intergenic | 35.0% |
| AGGAAAATGTGGAAACTGCA+TGG | + | 14450:6529-6548 | MS.gene58355:CDS | 40.0% | |
| GAAGATTTATCAGGGCAACT+TGG | + | 14450:6645-6664 | MS.gene58355:CDS | 40.0% | |
| GGAAAATGTGGAAACTGCAT+GGG | + | 14450:6530-6549 | MS.gene58355:CDS | 40.0% | |
| TCTTTGCACTGAGCAAGAAA+GGG | - | 14450:6244-6263 | MS.gene58355:intergenic | 40.0% | |
| TGAGGCTGATATGAATGTTG+TGG | + | 14450:6599-6618 | MS.gene58355:CDS | 40.0% | |
| !! | AACTTTAGCAGCATTGGTAG+TGG | - | 14450:6278-6297 | MS.gene58355:intergenic | 40.0% |
| !! | CTAGAGAGTGTTGAAGAAGT+TGG | + | 14450:6504-6523 | MS.gene58355:CDS | 40.0% |
| !! | GAGTGTTGAAGAAGTTGGTA+AGG | + | 14450:6509-6528 | MS.gene58355:CDS | 40.0% |
| AACTTGGAGATGGCTGTGAT+AGG | + | 14450:6661-6680 | MS.gene58355:CDS | 45.0% | |
| ATTCAGGGTGGTGATCCATT+AGG | + | 14450:6444-6463 | MS.gene58355:intron | 45.0% | |
| ATTCATATCAGCCTCAGCAG+TGG | - | 14450:6595-6614 | MS.gene58355:intergenic | 45.0% | |
| GTCTTTGCACTGAGCAAGAA+AGG | - | 14450:6245-6264 | MS.gene58355:intergenic | 45.0% | |
| TCTTGCTCAGTGCAAAGACT+TGG | + | 14450:6246-6265 | MS.gene58355:CDS | 45.0% | |
| TTATCAGGGCAACTTGGAGA+TGG | + | 14450:6651-6670 | MS.gene58355:CDS | 45.0% | |
| !!! | TTTTTTTTTTTTTAAATTCA+GGG | + | 14450:6429-6448 | MS.gene58355:intron | 5.0% |
| !!! | TTTTTTTTTTTTTTAAATTC+AGG | + | 14450:6428-6447 | MS.gene58355:intron | 5.0% |
| AGGGTCAGAGAAAGCAAGAG+AGG | + | 14450:6479-6498 | MS.gene58355:CDS | 50.0% | |
| GACTGAGACAACCACTGCTG+AGG | + | 14450:6581-6600 | MS.gene58355:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 14450 | gene | 6226 | 6695 | 6226 | ID=MS.gene58355 |
| 14450 | mRNA | 6226 | 6695 | 6226 | ID=MS.gene58355.t1;Parent=MS.gene58355 |
| 14450 | exon | 6226 | 6339 | 6226 | ID=MS.gene58355.t1.exon1;Parent=MS.gene58355.t1 |
| 14450 | CDS | 6226 | 6339 | 6226 | ID=cds.MS.gene58355.t1;Parent=MS.gene58355.t1 |
| 14450 | exon | 6450 | 6695 | 6450 | ID=MS.gene58355.t1.exon2;Parent=MS.gene58355.t1 |
| 14450 | CDS | 6450 | 6695 | 6450 | ID=cds.MS.gene58355.t1;Parent=MS.gene58355.t1 |
| Gene Sequence |
| Protein sequence |
