Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059776.t1 | XP_013464663.1 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.40E-50 | 208.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059776.t1 | A0A072VB72 | 92.4 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.0e-50 | 208.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05474 | MS.gene059776 | 0.807829 | 4.18E-50 | -1.69E-46 |
| MS.gene059776 | MS.gene58353 | 0.993884 | 6.13E-203 | -1.69E-46 |
| MS.gene059776 | MS.gene58355 | 0.998467 | 6.27E-266 | -1.69E-46 |
| MS.gene059776 | MS.gene67045 | 0.99294 | 2.07E-196 | -1.69E-46 |
| MS.gene059776 | MS.gene71246 | 0.994047 | 3.67E-204 | -1.69E-46 |
| MS.gene059776 | MS.gene71278 | 0.99255 | 5.71E-194 | -1.69E-46 |
| MS.gene059776 | MS.gene71279 | 0.998418 | 1.69E-264 | -1.69E-46 |
| MS.gene059776 | MS.gene71882 | 0.996948 | 1.45E-234 | -1.69E-46 |
| MS.gene059776 | MS.gene98856 | 0.818331 | 2.07E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059776.t1 | MTR_2g078780 | 92.437 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.50e-76 | 222 |
| MS.gene059776.t1 | MTR_1g061895 | 93.939 | 99 | 6 | 0 | 1 | 99 | 1 | 99 | 4.04e-63 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGATTTATTGCTCCTAA+TGG | 0.262425 | 2.4:-19644038 | None:intergenic |
| AGAAGTAGTGAAGATTTATC+AGG | 0.364128 | 2.4:+19644215 | MS.gene059776:CDS |
| AACAAAGAAAACTGTACAAT+TGG | 0.403825 | 2.4:+19644136 | MS.gene059776:CDS |
| GTCTTTGCACTGAGCAAGAA+AGG | 0.411430 | 2.4:-19643815 | None:intergenic |
| ATTCAGGGTGGTGATCCATT+AGG | 0.431783 | 2.4:+19644023 | MS.gene059776:intron |
| GTTGATAACTTTAGCAGCAT+TGG | 0.452113 | 2.4:-19643854 | None:intergenic |
| CTAGAGAGTGTTGAAGAAGT+TGG | 0.457188 | 2.4:+19644083 | MS.gene059776:CDS |
| GAGTGTTGAAGAAGTTGGTA+AGG | 0.463197 | 2.4:+19644088 | MS.gene059776:CDS |
| TCTTGCTCAGTGCAAAGACT+TGG | 0.489170 | 2.4:+19643819 | MS.gene059776:CDS |
| AACTTGGAGATGGCTGTGAT+AGG | 0.503601 | 2.4:+19644240 | MS.gene059776:CDS |
| AACTTTAGCAGCATTGGTAG+TGG | 0.505111 | 2.4:-19643848 | None:intergenic |
| CCATTAGGAGCAATAAATCA+AGG | 0.506320 | 2.4:+19644038 | MS.gene059776:CDS |
| GAAGATTTATCAGGGCAACT+TGG | 0.516603 | 2.4:+19644224 | MS.gene059776:CDS |
| AGGAAAATGTGGAAACTGCA+TGG | 0.519693 | 2.4:+19644108 | MS.gene059776:CDS |
| TTATCAGGGCAACTTGGAGA+TGG | 0.537867 | 2.4:+19644230 | MS.gene059776:CDS |
| AGAAGTTGGTAAGGAAAATG+TGG | 0.552960 | 2.4:+19644097 | MS.gene059776:CDS |
| GAAGTAGTGAAGATTTATCA+GGG | 0.567179 | 2.4:+19644216 | MS.gene059776:CDS |
| TCTTTGCACTGAGCAAGAAA+GGG | 0.570085 | 2.4:-19643814 | None:intergenic |
| AGGGTCAGAGAAAGCAAGAG+AGG | 0.619602 | 2.4:+19644058 | MS.gene059776:CDS |
| GACTGAGACAACCACTGCTG+AGG | 0.629251 | 2.4:+19644160 | MS.gene059776:CDS |
| TGAGGCTGATATGAATGTTG+TGG | 0.639431 | 2.4:+19644178 | MS.gene059776:CDS |
| CATTAGGAGCAATAAATCAA+GGG | 0.647640 | 2.4:+19644039 | MS.gene059776:CDS |
| ATTCATATCAGCCTCAGCAG+TGG | 0.674496 | 2.4:-19644171 | None:intergenic |
| GGAAAATGTGGAAACTGCAT+GGG | 0.686615 | 2.4:+19644109 | MS.gene059776:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTGAAAGAAAAAAAATTG+AGG | - | chr2.4:19643921-19643940 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTTAAATTCAGGG+TGG | + | chr2.4:19644011-19644030 | MS.gene059776:intron | 20.0% |
| ! | AACAAAGAAAACTGTACAAT+TGG | + | chr2.4:19644136-19644155 | MS.gene059776:CDS | 25.0% |
| AGAAGTAGTGAAGATTTATC+AGG | + | chr2.4:19644215-19644234 | MS.gene059776:CDS | 30.0% | |
| CATTAGGAGCAATAAATCAA+GGG | + | chr2.4:19644039-19644058 | MS.gene059776:CDS | 30.0% | |
| GAAGTAGTGAAGATTTATCA+GGG | + | chr2.4:19644216-19644235 | MS.gene059776:CDS | 30.0% | |
| CCATTAGGAGCAATAAATCA+AGG | + | chr2.4:19644038-19644057 | MS.gene059776:CDS | 35.0% | |
| CCTTGATTTATTGCTCCTAA+TGG | - | chr2.4:19644041-19644060 | None:intergenic | 35.0% | |
| !! | AGAAGTTGGTAAGGAAAATG+TGG | + | chr2.4:19644097-19644116 | MS.gene059776:CDS | 35.0% |
| !! | GTTGATAACTTTAGCAGCAT+TGG | - | chr2.4:19643857-19643876 | None:intergenic | 35.0% |
| AGGAAAATGTGGAAACTGCA+TGG | + | chr2.4:19644108-19644127 | MS.gene059776:CDS | 40.0% | |
| GAAGATTTATCAGGGCAACT+TGG | + | chr2.4:19644224-19644243 | MS.gene059776:CDS | 40.0% | |
| GGAAAATGTGGAAACTGCAT+GGG | + | chr2.4:19644109-19644128 | MS.gene059776:CDS | 40.0% | |
| TCTTTGCACTGAGCAAGAAA+GGG | - | chr2.4:19643817-19643836 | None:intergenic | 40.0% | |
| TGAGGCTGATATGAATGTTG+TGG | + | chr2.4:19644178-19644197 | MS.gene059776:CDS | 40.0% | |
| !! | AACTTTAGCAGCATTGGTAG+TGG | - | chr2.4:19643851-19643870 | None:intergenic | 40.0% |
| !! | CTAGAGAGTGTTGAAGAAGT+TGG | + | chr2.4:19644083-19644102 | MS.gene059776:CDS | 40.0% |
| !! | GAGTGTTGAAGAAGTTGGTA+AGG | + | chr2.4:19644088-19644107 | MS.gene059776:CDS | 40.0% |
| AACTTGGAGATGGCTGTGAT+AGG | + | chr2.4:19644240-19644259 | MS.gene059776:CDS | 45.0% | |
| ATTCAGGGTGGTGATCCATT+AGG | + | chr2.4:19644023-19644042 | MS.gene059776:intron | 45.0% | |
| ATTCATATCAGCCTCAGCAG+TGG | - | chr2.4:19644174-19644193 | None:intergenic | 45.0% | |
| GTCTTTGCACTGAGCAAGAA+AGG | - | chr2.4:19643818-19643837 | None:intergenic | 45.0% | |
| TCTTGCTCAGTGCAAAGACT+TGG | + | chr2.4:19643819-19643838 | MS.gene059776:CDS | 45.0% | |
| TTATCAGGGCAACTTGGAGA+TGG | + | chr2.4:19644230-19644249 | MS.gene059776:CDS | 45.0% | |
| !!! | TTTTTTTTTTTTTAAATTCA+GGG | + | chr2.4:19644008-19644027 | MS.gene059776:intron | 5.0% |
| !!! | TTTTTTTTTTTTTTAAATTC+AGG | + | chr2.4:19644007-19644026 | MS.gene059776:intron | 5.0% |
| AGGGTCAGAGAAAGCAAGAG+AGG | + | chr2.4:19644058-19644077 | MS.gene059776:CDS | 50.0% | |
| GACTGAGACAACCACTGCTG+AGG | + | chr2.4:19644160-19644179 | MS.gene059776:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 19643799 | 19644274 | 19643799 | ID=MS.gene059776 |
| chr2.4 | mRNA | 19643799 | 19644274 | 19643799 | ID=MS.gene059776.t1;Parent=MS.gene059776 |
| chr2.4 | exon | 19643799 | 19643912 | 19643799 | ID=MS.gene059776.t1.exon1;Parent=MS.gene059776.t1 |
| chr2.4 | CDS | 19643799 | 19643912 | 19643799 | ID=cds.MS.gene059776.t1;Parent=MS.gene059776.t1 |
| chr2.4 | exon | 19644029 | 19644274 | 19644029 | ID=MS.gene059776.t1.exon2;Parent=MS.gene059776.t1 |
| chr2.4 | CDS | 19644029 | 19644274 | 19644029 | ID=cds.MS.gene059776.t1;Parent=MS.gene059776.t1 |
| Gene Sequence |
| Protein sequence |
