AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58427

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58427.t1 MTR_5g014710 64.968 314 108 1 90 403 47 358 5.88e-146 419
MS.gene58427.t1 MTR_4g092690 65.079 315 108 1 89 403 44 356 1.51e-141 407
MS.gene58427.t1 MTR_8g463760 60.121 331 126 3 77 403 15 343 1.86e-139 402
MS.gene58427.t1 MTR_8g463760 63.547 203 71 1 77 276 15 217 3.66e-88 268
MS.gene58427.t1 MTR_2g021700 72.549 51 14 0 256 306 52 102 4.04e-16 75.5
MS.gene58427.t1 MTR_2g045010 72.549 51 14 0 256 306 52 102 4.04e-16 75.5
MS.gene58427.t1 MTR_4g033285 73.333 45 12 0 256 300 52 96 1.45e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58427.t1 AT3G47520 77.309 379 76 4 20 398 23 391 0.0 573
MS.gene58427.t1 AT1G53240 64.537 313 109 1 91 403 31 341 2.71e-144 414
MS.gene58427.t1 AT2G22780 63.609 327 117 1 77 403 30 354 1.13e-143 413
MS.gene58427.t1 AT3G15020 63.259 313 113 1 91 403 31 341 1.47e-140 404
MS.gene58427.t1 AT5G09660 60.870 345 129 3 63 403 12 354 1.64e-135 392
MS.gene58427.t1 AT5G09660 64.762 315 109 1 89 403 21 333 3.87e-135 390
MS.gene58427.t1 AT5G09660 60.580 345 129 4 63 403 12 353 3.54e-133 386
MS.gene58427.t1 AT3G15020 64.338 272 97 0 91 362 31 302 9.82e-125 363
MS.gene58427.t1 AT5G09660 63.322 289 102 2 63 347 12 300 6.41e-117 345
MS.gene58427.t1 AT5G09660 68.605 258 81 0 90 347 22 279 2.05e-116 343
MS.gene58427.t1 AT3G53910 49.438 89 45 0 318 406 9 97 2.55e-26 102

Find 108 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTCAATCTAGTCCTTT+TGG 0.231181 8.3:+15872634 MS.gene58427:CDS
CAACACCCCTTCGAAAGTTT+TGG 0.243573 8.3:+15872984 MS.gene58427:CDS
GAAACACCAAAGAGCTTCTT+AGG 0.295270 8.3:-15873258 None:intergenic
GCAAGTTGCGGGTGAGGTTT+CGG 0.332641 8.3:-15872807 None:intergenic
GAGGAAGTGACCTCCCTGTT+TGG 0.346298 8.3:-15872618 None:intergenic
GTTAATTTCAACACCTTCTC+TGG 0.351785 8.3:+15872676 MS.gene58427:CDS
ACTATTGGAACCACCCAAAC+AGG 0.361628 8.3:+15872604 MS.gene58427:CDS
AGCGGCACAAGTTTCTTTGC+CGG 0.363971 8.3:-15872746 None:intergenic
TCGATGCTGGCCTTAAGTTC+TGG 0.369041 8.3:-15873738 None:intergenic
AGGAAGTGACCTCCCTGTTT+GGG 0.375677 8.3:-15872617 None:intergenic
TCTCATCAAGATGTCGCCTT+TGG 0.378753 8.3:+15872897 MS.gene58427:CDS
CTTGGTGCTGCAGGAGGGAT+TGG 0.382446 8.3:+15872859 MS.gene58427:CDS
ATAATATGAATAAAAGCATT+GGG 0.384767 8.3:-15873168 None:intergenic
TGAGTATGAGCAGAAGGCTT+TGG 0.385556 8.3:+15873704 MS.gene58427:CDS
CGAGTCATGCCGGGCTTCCT+AGG 0.395727 8.3:-15873078 None:intergenic
TTTAGAATTTCAGCGGCAAT+AGG 0.400849 8.3:-15873216 None:intergenic
TCCTACAAAGTAGCGGTTCT+TGG 0.402994 8.3:+15872841 MS.gene58427:CDS
CTATGATATCGCGAATGTTA+AGG 0.403817 8.3:+15872936 MS.gene58427:CDS
GTTGAGGCGAAGGCTGGTGC+AGG 0.406244 8.3:+15873483 MS.gene58427:CDS
TGCCTGAGGCTGCAAGTTGC+GGG 0.408008 8.3:-15872818 None:intergenic
TATGATATCGCGAATGTTAA+GGG 0.410197 8.3:+15872937 MS.gene58427:CDS
TCGTCTCTTCGTGCACTTGA+CGG 0.411087 8.3:+15873552 MS.gene58427:CDS
CAGTTGTTGGAGGTCATGCC+GGG 0.417388 8.3:+15873355 MS.gene58427:CDS
ACCAAGAACCGCTACTTTGT+AGG 0.417991 8.3:-15872842 None:intergenic
CACCCGCAACTTGCAGCCTC+AGG 0.423294 8.3:+15872816 MS.gene58427:CDS
CCGGCATGACCTCCAACAAC+TGG 0.427912 8.3:-15873354 None:intergenic
ACATCAATGCTGGTATAGTC+AGG 0.428901 8.3:+15873115 MS.gene58427:CDS
TTATCCCTGCTGGTGTTCCT+AGG 0.429918 8.3:+15873061 MS.gene58427:CDS
GGCTTCCTAGGAACACCAGC+AGG 0.432243 8.3:-15873066 None:intergenic
AGCACTTAAGCCAGAACTTA+AGG 0.432437 8.3:+15873728 MS.gene58427:CDS
TGAGAGATGGCATGGCTTTG+AGG 0.432465 8.3:-15872699 None:intergenic
TATGATCCTAAGAAGCTCTT+TGG 0.434339 8.3:+15873252 MS.gene58427:CDS
AGGGTCTGCTACTTTGTCAA+TGG 0.435055 8.3:+15873503 MS.gene58427:CDS
GAGGCTGCAAGTTGCGGGTG+AGG 0.436560 8.3:-15872813 None:intergenic
GATGTGGATGTTCCAGTTGT+TGG 0.440014 8.3:+15873342 MS.gene58427:CDS
GTAGCGGTTCTTGGTGCTGC+AGG 0.442098 8.3:+15872850 MS.gene58427:CDS
CTCAATCCTTGGAGATCAGT+TGG 0.450054 8.3:-15873681 None:intergenic
GATAATATGAATAAAAGCAT+TGG 0.454366 8.3:-15873169 None:intergenic
CCAGTTGTTGGAGGTCATGC+CGG 0.456311 8.3:+15873354 MS.gene58427:CDS
TTTCACTGATGAAGAAATTG+AGG 0.470058 8.3:+15873422 MS.gene58427:CDS
ATGCCTGAGGCTGCAAGTTG+CGG 0.479068 8.3:-15872819 None:intergenic
GCTCATACTCACTCAATCCT+TGG 0.489832 8.3:-15873692 None:intergenic
GATGTTGTTGTTATCCCTGC+TGG 0.490735 8.3:+15873051 MS.gene58427:CDS
GAATTGAATTTCAAACCAAA+AGG 0.499197 8.3:-15872649 None:intergenic
TGGTTTGAAATTCAATTCCC+AGG 0.499844 8.3:+15872654 MS.gene58427:CDS
GACTCTGAATCATCTTTCTC+CGG 0.500629 8.3:+15872727 MS.gene58427:CDS
CTTCAAGGGTGAAGATTGGT+AGG 0.501008 8.3:+15873637 MS.gene58427:CDS
TACCAGCATTGATGTTGAAA+AGG 0.504347 8.3:-15873107 None:intergenic
TTTGCTTCAAGGGTGAAGAT+TGG 0.507205 8.3:+15873633 MS.gene58427:CDS
GAGTTAGCAAATTGTTTGAA+AGG 0.510601 8.3:+15873024 MS.gene58427:CDS
AAGGTCGTCACGAGTCATGC+CGG 0.512854 8.3:-15873088 None:intergenic
AAACCAAAAGGACTAGATTG+AGG 0.513013 8.3:-15872637 None:intergenic
AGCAAATTGTTTGAAAGGTG+TGG 0.519677 8.3:+15873029 MS.gene58427:CDS
ATAGGAACTGTAGAGTTCAC+TGG 0.520893 8.3:-15873198 None:intergenic
CGCTGAAATTCTAAAACAGA+AGG 0.523131 8.3:+15873224 MS.gene58427:CDS
AGGAGGGATTGGTCAGCCAC+TGG 0.524866 8.3:+15872870 MS.gene58427:CDS
CGCTGGAACTGAGGTTGTTG+AGG 0.525890 8.3:+15873467 MS.gene58427:CDS
TGCTGGTATAGTCAGGGACT+TGG 0.529987 8.3:+15873122 MS.gene58427:CDS
GTGGATGTTCCAGTTGTTGG+AGG 0.532818 8.3:+15873345 MS.gene58427:CDS
GCGGTTCTTGGTGCTGCAGG+AGG 0.533048 8.3:+15872853 MS.gene58427:CDS
GTGAAATCCAAAACTTTCGA+AGG 0.537192 8.3:-15872991 None:intergenic
TCGCGATATCATAGAGATGC+AGG 0.542453 8.3:-15872927 None:intergenic
AGGTCGTCACGAGTCATGCC+GGG 0.542713 8.3:-15873087 None:intergenic
AGGGTGAAGATTGGTAGGAA+AGG 0.542764 8.3:+15873642 MS.gene58427:CDS
AAGTGACCTCCCTGTTTGGG+TGG 0.544528 8.3:-15872614 None:intergenic
GAGAGGAAGAATTGTAATCC+CGG 0.547677 8.3:-15873373 None:intergenic
AGAAGGTGTTGAAATTAACC+TGG 0.550640 8.3:-15872672 None:intergenic
TTTCTACACTTGATGTTGTG+AGG 0.552915 8.3:+15873277 MS.gene58427:CDS
GAGGTTGTTGAGGCGAAGGC+TGG 0.553690 8.3:+15873477 MS.gene58427:CDS
ATTGAGTGAGTATGAGCAGA+AGG 0.555968 8.3:+15873698 MS.gene58427:CDS
AACTGAGGTTGTTGAGGCGA+AGG 0.556107 8.3:+15873473 MS.gene58427:CDS
TTAATTCCAACTGATCTCCA+AGG 0.558509 8.3:+15873675 MS.gene58427:CDS
GATTCAAAACGCTGGAACTG+AGG 0.561075 8.3:+15873458 MS.gene58427:CDS
GCTGGTGTTCCTAGGAAGCC+CGG 0.561452 8.3:+15873069 MS.gene58427:CDS
CTTAAGGCCAGCATCGAGAA+GGG 0.563554 8.3:+15873744 MS.gene58427:CDS
TCATCAGTGAAACTCACTGA+GGG 0.567339 8.3:-15873411 None:intergenic
AAATTGAGGAGCTAACTGTC+AGG 0.577890 8.3:+15873436 MS.gene58427:CDS
GTCACATCTGAGAGATGGCA+TGG 0.579241 8.3:-15872707 None:intergenic
ACTTAAGGCCAGCATCGAGA+AGG 0.580005 8.3:+15873743 MS.gene58427:CDS
CGGTTCTTGGTGCTGCAGGA+GGG 0.586824 8.3:+15872854 MS.gene58427:CDS
GAACCTGAGGTTGATTGATG+TGG 0.587092 8.3:+15873326 MS.gene58427:CDS
TTCTACACTTGATGTTGTGA+GGG 0.588106 8.3:+15873278 MS.gene58427:CDS
CATCCACATCAATCAACCTC+AGG 0.589306 8.3:-15873329 None:intergenic
TGAAATCCAAAACTTTCGAA+GGG 0.589668 8.3:-15872990 None:intergenic
CATGGCTTTGAGGCCAGAGA+AGG 0.592005 8.3:-15872689 None:intergenic
TCAGAGTCACATCTGAGAGA+TGG 0.601214 8.3:-15872712 None:intergenic
TTGAGGCGAAGGCTGGTGCA+GGG 0.614890 8.3:+15873484 MS.gene58427:CDS
ATTGGGGCAATTATCTGCAA+CGG 0.623966 8.3:-15873151 None:intergenic
TTGCTCAGAAGAAGAACCTG+AGG 0.625392 8.3:+15873313 MS.gene58427:CDS
CATCAATGCTGGTATAGTCA+GGG 0.635474 8.3:+15873116 MS.gene58427:CDS
CTATTGGAACCACCCAAACA+GGG 0.635885 8.3:+15872605 MS.gene58427:CDS
AGATGCAGGTCGGAAACCAA+AGG 0.636274 8.3:-15872913 None:intergenic
TGCAAAAGTTGCACGCAGAG+CGG 0.636866 8.3:-15872764 None:intergenic
GAAGGTGTTGAAATTAACCT+GGG 0.642084 8.3:-15872671 None:intergenic
GCTGAAATTCTAAAACAGAA+GGG 0.647110 8.3:+15873225 MS.gene58427:CDS
TTAAGGCCAGCATCGAGAAG+GGG 0.649629 8.3:+15873745 MS.gene58427:CDS
TAAGGCCAGCATCGAGAAGG+GGG 0.655068 8.3:+15873746 MS.gene58427:CDS
ACTGTCAGGATTCAAAACGC+TGG 0.660417 8.3:+15873450 MS.gene58427:CDS
TAATATGAATAAAAGCATTG+GGG 0.668981 8.3:-15873167 None:intergenic
GATTGGTAGGAAAGGAGTCG+AGG 0.670539 8.3:+15873650 MS.gene58427:CDS
GCTTCCTAGGAACACCAGCA+GGG 0.676922 8.3:-15873065 None:intergenic
TTCATCAGTGAAACTCACTG+AGG 0.681230 8.3:-15873412 None:intergenic
TCAGGCATCCTACAAAGTAG+CGG 0.690931 8.3:+15872834 MS.gene58427:CDS
GAAATCCAAAACTTTCGAAG+GGG 0.694170 8.3:-15872989 None:intergenic
GCTACTTTGTAGGATGCCTG+AGG 0.706674 8.3:-15872832 None:intergenic
GATATCATAGAGATGCAGGT+CGG 0.718519 8.3:-15872923 None:intergenic
ATCTTGATGAGAAGTGCCAG+TGG 0.724135 8.3:-15872886 None:intergenic
TTGGAACCACCCAAACAGGG+AGG 0.754881 8.3:+15872608 MS.gene58427:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATGAATAAAAGCATT+GGG - chr8.3:15873171-15873190 None:intergenic 15.0%
!!! GATAATATGAATAAAAGCAT+TGG - chr8.3:15873172-15873191 None:intergenic 20.0%
!!! TAATATGAATAAAAGCATTG+GGG - chr8.3:15873170-15873189 None:intergenic 20.0%
! GAATTGAATTTCAAACCAAA+AGG - chr8.3:15872652-15872671 None:intergenic 25.0%
GAGTTAGCAAATTGTTTGAA+AGG + chr8.3:15873024-15873043 MS.gene58427:CDS 30.0%
TATGATATCGCGAATGTTAA+GGG + chr8.3:15872937-15872956 MS.gene58427:CDS 30.0%
TGAAATCCAAAACTTTCGAA+GGG - chr8.3:15872993-15873012 None:intergenic 30.0%
TTTCACTGATGAAGAAATTG+AGG + chr8.3:15873422-15873441 MS.gene58427:CDS 30.0%
!! GCTGAAATTCTAAAACAGAA+GGG + chr8.3:15873225-15873244 MS.gene58427:CDS 30.0%
!!! CTTCTGTTTTAGAATTTCAG+CGG - chr8.3:15873226-15873245 None:intergenic 30.0%
AAACCAAAAGGACTAGATTG+AGG - chr8.3:15872640-15872659 None:intergenic 35.0%
AGCAAATTGTTTGAAAGGTG+TGG + chr8.3:15873029-15873048 MS.gene58427:CDS 35.0%
CTATGATATCGCGAATGTTA+AGG + chr8.3:15872936-15872955 MS.gene58427:CDS 35.0%
GAAATCCAAAACTTTCGAAG+GGG - chr8.3:15872992-15873011 None:intergenic 35.0%
GTGAAATCCAAAACTTTCGA+AGG - chr8.3:15872994-15873013 None:intergenic 35.0%
GTTAATTTCAACACCTTCTC+TGG + chr8.3:15872676-15872695 MS.gene58427:CDS 35.0%
TATGATCCTAAGAAGCTCTT+TGG + chr8.3:15873252-15873271 MS.gene58427:CDS 35.0%
TTAATTCCAACTGATCTCCA+AGG + chr8.3:15873675-15873694 MS.gene58427:CDS 35.0%
TTCACCCTTGAAGCAAAAAA+CGG - chr8.3:15873630-15873649 None:intergenic 35.0%
TTCTACACTTGATGTTGTGA+GGG + chr8.3:15873278-15873297 MS.gene58427:CDS 35.0%
TTTCTACACTTGATGTTGTG+AGG + chr8.3:15873277-15873296 MS.gene58427:CDS 35.0%
! TGGTTTGAAATTCAATTCCC+AGG + chr8.3:15872654-15872673 MS.gene58427:CDS 35.0%
! TTTAGAATTTCAGCGGCAAT+AGG - chr8.3:15873219-15873238 None:intergenic 35.0%
!! AGAAGGTGTTGAAATTAACC+TGG - chr8.3:15872675-15872694 None:intergenic 35.0%
!! CGCTGAAATTCTAAAACAGA+AGG + chr8.3:15873224-15873243 MS.gene58427:CDS 35.0%
!! GAAGGTGTTGAAATTAACCT+GGG - chr8.3:15872674-15872693 None:intergenic 35.0%
!! TACCAGCATTGATGTTGAAA+AGG - chr8.3:15873110-15873129 None:intergenic 35.0%
!!! GCATCAGCTACTTTTACTAT+TGG + chr8.3:15872589-15872608 MS.gene58427:CDS 35.0%
!!! TCGAAAGTTTTGGATTTCAC+AGG + chr8.3:15872994-15873013 MS.gene58427:CDS 35.0%
AAATTGAGGAGCTAACTGTC+AGG + chr8.3:15873436-15873455 MS.gene58427:CDS 40.0%
ACATCAATGCTGGTATAGTC+AGG + chr8.3:15873115-15873134 MS.gene58427:CDS 40.0%
AGCACTTAAGCCAGAACTTA+AGG + chr8.3:15873728-15873747 MS.gene58427:CDS 40.0%
ATAGGAACTGTAGAGTTCAC+TGG - chr8.3:15873201-15873220 None:intergenic 40.0%
ATTGAGTGAGTATGAGCAGA+AGG + chr8.3:15873698-15873717 MS.gene58427:CDS 40.0%
ATTGGGGCAATTATCTGCAA+CGG - chr8.3:15873154-15873173 None:intergenic 40.0%
CATCAATGCTGGTATAGTCA+GGG + chr8.3:15873116-15873135 MS.gene58427:CDS 40.0%
GAAACACCAAAGAGCTTCTT+AGG - chr8.3:15873261-15873280 None:intergenic 40.0%
GACTCTGAATCATCTTTCTC+CGG + chr8.3:15872727-15872746 MS.gene58427:CDS 40.0%
GAGAGGAAGAATTGTAATCC+CGG - chr8.3:15873376-15873395 None:intergenic 40.0%
GATATCATAGAGATGCAGGT+CGG - chr8.3:15872926-15872945 None:intergenic 40.0%
TCATCAGTGAAACTCACTGA+GGG - chr8.3:15873414-15873433 None:intergenic 40.0%
TGAAGCAAAAAACGGAAGGT+CGG - chr8.3:15873622-15873641 None:intergenic 40.0%
TTCATCAGTGAAACTCACTG+AGG - chr8.3:15873415-15873434 None:intergenic 40.0%
TTTGCTTCAAGGGTGAAGAT+TGG + chr8.3:15873633-15873652 MS.gene58427:CDS 40.0%
! CGGTTTTCCTTTTGAGCTTT+GGG - chr8.3:15872790-15872809 None:intergenic 40.0%
! CTTCCTCAATCTAGTCCTTT+TGG + chr8.3:15872634-15872653 MS.gene58427:CDS 40.0%
! GACCTTTTCAACATCAATGC+TGG + chr8.3:15873105-15873124 MS.gene58427:CDS 40.0%
! TCGGTTTTCCTTTTGAGCTT+TGG - chr8.3:15872791-15872810 None:intergenic 40.0%
! TTTTGCACCCAAAGCTCAAA+AGG + chr8.3:15872780-15872799 MS.gene58427:CDS 40.0%
!! GGTTTTGTCTTTGACAAGAG+AGG - chr8.3:15873393-15873412 None:intergenic 40.0%
!!! ACCTTCCGTTTTTTGCTTCA+AGG + chr8.3:15873622-15873641 MS.gene58427:CDS 40.0%
!!! CCTTCCGTTTTTTGCTTCAA+GGG + chr8.3:15873623-15873642 MS.gene58427:CDS 40.0%
ACTATTGGAACCACCCAAAC+AGG + chr8.3:15872604-15872623 MS.gene58427:CDS 45.0%
ACTGTCAGGATTCAAAACGC+TGG + chr8.3:15873450-15873469 MS.gene58427:CDS 45.0%
AGGGTGAAGATTGGTAGGAA+AGG + chr8.3:15873642-15873661 MS.gene58427:CDS 45.0%
CATCCACATCAATCAACCTC+AGG - chr8.3:15873332-15873351 None:intergenic 45.0%
CCCTTGAAGCAAAAAACGGA+AGG - chr8.3:15873626-15873645 None:intergenic 45.0%
CTATTGGAACCACCCAAACA+GGG + chr8.3:15872605-15872624 MS.gene58427:CDS 45.0%
CTCAATCCTTGGAGATCAGT+TGG - chr8.3:15873684-15873703 None:intergenic 45.0%
CTTCAAGGGTGAAGATTGGT+AGG + chr8.3:15873637-15873656 MS.gene58427:CDS 45.0%
GATGTGGATGTTCCAGTTGT+TGG + chr8.3:15873342-15873361 MS.gene58427:CDS 45.0%
GATTCAAAACGCTGGAACTG+AGG + chr8.3:15873458-15873477 MS.gene58427:CDS 45.0%
GCTCATACTCACTCAATCCT+TGG - chr8.3:15873695-15873714 None:intergenic 45.0%
TCAGAGTCACATCTGAGAGA+TGG - chr8.3:15872715-15872734 None:intergenic 45.0%
TCAGGCATCCTACAAAGTAG+CGG + chr8.3:15872834-15872853 MS.gene58427:CDS 45.0%
TCCTACAAAGTAGCGGTTCT+TGG + chr8.3:15872841-15872860 MS.gene58427:CDS 45.0%
TCGCGATATCATAGAGATGC+AGG - chr8.3:15872930-15872949 None:intergenic 45.0%
TCTCATCAAGATGTCGCCTT+TGG + chr8.3:15872897-15872916 MS.gene58427:CDS 45.0%
TGAGTATGAGCAGAAGGCTT+TGG + chr8.3:15873704-15873723 MS.gene58427:CDS 45.0%
TTGCTCAGAAGAAGAACCTG+AGG + chr8.3:15873313-15873332 MS.gene58427:CDS 45.0%
! ACCAAGAACCGCTACTTTGT+AGG - chr8.3:15872845-15872864 None:intergenic 45.0%
! CAACACCCCTTCGAAAGTTT+TGG + chr8.3:15872984-15873003 MS.gene58427:CDS 45.0%
! GAACCTGAGGTTGATTGATG+TGG + chr8.3:15873326-15873345 MS.gene58427:CDS 45.0%
! GATGTTGTTGTTATCCCTGC+TGG + chr8.3:15873051-15873070 MS.gene58427:CDS 45.0%
!! AGGGTCTGCTACTTTGTCAA+TGG + chr8.3:15873503-15873522 MS.gene58427:CDS 45.0%
!! ATCTTGATGAGAAGTGCCAG+TGG - chr8.3:15872889-15872908 None:intergenic 45.0%
AACTGAGGTTGTTGAGGCGA+AGG + chr8.3:15873473-15873492 MS.gene58427:CDS 50.0%
AGATGCAGGTCGGAAACCAA+AGG - chr8.3:15872916-15872935 None:intergenic 50.0%
AGCGGCACAAGTTTCTTTGC+CGG - chr8.3:15872749-15872768 None:intergenic 50.0%
AGGAAGTGACCTCCCTGTTT+GGG - chr8.3:15872620-15872639 None:intergenic 50.0%
GATTGGTAGGAAAGGAGTCG+AGG + chr8.3:15873650-15873669 MS.gene58427:CDS 50.0%
GTCACATCTGAGAGATGGCA+TGG - chr8.3:15872710-15872729 None:intergenic 50.0%
GTGGATGTTCCAGTTGTTGG+AGG + chr8.3:15873345-15873364 MS.gene58427:CDS 50.0%
TCGTCTCTTCGTGCACTTGA+CGG + chr8.3:15873552-15873571 MS.gene58427:CDS 50.0%
TGCAAAAGTTGCACGCAGAG+CGG - chr8.3:15872767-15872786 None:intergenic 50.0%
TGCTGGTATAGTCAGGGACT+TGG + chr8.3:15873122-15873141 MS.gene58427:CDS 50.0%
TTATCCCTGCTGGTGTTCCT+AGG + chr8.3:15873061-15873080 MS.gene58427:CDS 50.0%
! ACTTAAGGCCAGCATCGAGA+AGG + chr8.3:15873743-15873762 MS.gene58427:CDS 50.0%
! CTTAAGGCCAGCATCGAGAA+GGG + chr8.3:15873744-15873763 MS.gene58427:CDS 50.0%
! GCTACTTTGTAGGATGCCTG+AGG - chr8.3:15872835-15872854 None:intergenic 50.0%
! TGAGAGATGGCATGGCTTTG+AGG - chr8.3:15872702-15872721 None:intergenic 50.0%
! TTAAGGCCAGCATCGAGAAG+GGG + chr8.3:15873745-15873764 MS.gene58427:CDS 50.0%
!! TCGATGCTGGCCTTAAGTTC+TGG - chr8.3:15873741-15873760 None:intergenic 50.0%
AAGGTCGTCACGAGTCATGC+CGG - chr8.3:15873091-15873110 None:intergenic 55.0%
AAGTGACCTCCCTGTTTGGG+TGG - chr8.3:15872617-15872636 None:intergenic 55.0%
ATGCCTGAGGCTGCAAGTTG+CGG - chr8.3:15872822-15872841 None:intergenic 55.0%
CAGTTGTTGGAGGTCATGCC+GGG + chr8.3:15873355-15873374 MS.gene58427:CDS 55.0%
CCAGTTGTTGGAGGTCATGC+CGG + chr8.3:15873354-15873373 MS.gene58427:CDS 55.0%
CGCTGGAACTGAGGTTGTTG+AGG + chr8.3:15873467-15873486 MS.gene58427:CDS 55.0%
GAGGAAGTGACCTCCCTGTT+TGG - chr8.3:15872621-15872640 None:intergenic 55.0%
GCAAGTTGCGGGTGAGGTTT+CGG - chr8.3:15872810-15872829 None:intergenic 55.0%
GCTTCCTAGGAACACCAGCA+GGG - chr8.3:15873068-15873087 None:intergenic 55.0%
TTGGAACCACCCAAACAGGG+AGG + chr8.3:15872608-15872627 MS.gene58427:CDS 55.0%
! TAAGGCCAGCATCGAGAAGG+GGG + chr8.3:15873746-15873765 MS.gene58427:CDS 55.0%
!! CATGGCTTTGAGGCCAGAGA+AGG - chr8.3:15872692-15872711 None:intergenic 55.0%
AGCAACCCCCTTCTCGATGC+TGG - chr8.3:15873754-15873773 None:intergenic 60.0%
AGGTCGTCACGAGTCATGCC+GGG - chr8.3:15873090-15873109 None:intergenic 60.0%
CCGGCATGACCTCCAACAAC+TGG - chr8.3:15873357-15873376 None:intergenic 60.0%
GAGGTTGTTGAGGCGAAGGC+TGG + chr8.3:15873477-15873496 MS.gene58427:CDS 60.0%
GCTGGTGTTCCTAGGAAGCC+CGG + chr8.3:15873069-15873088 MS.gene58427:CDS 60.0%
GGCTTCCTAGGAACACCAGC+AGG - chr8.3:15873069-15873088 None:intergenic 60.0%
TGCCTGAGGCTGCAAGTTGC+GGG - chr8.3:15872821-15872840 None:intergenic 60.0%
TTGAGGCGAAGGCTGGTGCA+GGG + chr8.3:15873484-15873503 MS.gene58427:CDS 60.0%
!! AGGAGGGATTGGTCAGCCAC+TGG + chr8.3:15872870-15872889 MS.gene58427:CDS 60.0%
!! CGGTTCTTGGTGCTGCAGGA+GGG + chr8.3:15872854-15872873 MS.gene58427:CDS 60.0%
!! CTTGGTGCTGCAGGAGGGAT+TGG + chr8.3:15872859-15872878 MS.gene58427:CDS 60.0%
!! GTAGCGGTTCTTGGTGCTGC+AGG + chr8.3:15872850-15872869 MS.gene58427:CDS 60.0%
CACCCGCAACTTGCAGCCTC+AGG + chr8.3:15872816-15872835 MS.gene58427:CDS 65.0%
CGAGTCATGCCGGGCTTCCT+AGG - chr8.3:15873081-15873100 None:intergenic 65.0%
GAGGCTGCAAGTTGCGGGTG+AGG - chr8.3:15872816-15872835 None:intergenic 65.0%
GTTGAGGCGAAGGCTGGTGC+AGG + chr8.3:15873483-15873502 MS.gene58427:CDS 65.0%
!! GCGGTTCTTGGTGCTGCAGG+AGG + chr8.3:15872853-15872872 MS.gene58427:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 15872577 15873803 15872577 ID=MS.gene58427
chr8.3 mRNA 15872577 15873803 15872577 ID=MS.gene58427.t1;Parent=MS.gene58427
chr8.3 exon 15872577 15873803 15872577 ID=MS.gene58427.t1.exon1;Parent=MS.gene58427.t1
chr8.3 CDS 15872577 15873803 15872577 ID=cds.MS.gene58427.t1;Parent=MS.gene58427.t1
Gene Sequence

>MS.gene58427

ATGGCGGCAGCAGCATCAGCTACTTTTACTATTGGAACCACCCAAACAGGGAGGTCACTTCCTCAATCTAGTCCTTTTGGTTTGAAATTCAATTCCCAGGTTAATTTCAACACCTTCTCTGGCCTCAAAGCCATGCCATCTCTCAGATGTGACTCTGAATCATCTTTCTCCGGCAAAGAAACTTGTGCCGCTCTGCGTGCAACTTTTGCACCCAAAGCTCAAAAGGAAAACCGAAACCTCACCCGCAACTTGCAGCCTCAGGCATCCTACAAAGTAGCGGTTCTTGGTGCTGCAGGAGGGATTGGTCAGCCACTGGCACTTCTCATCAAGATGTCGCCTTTGGTTTCCGACCTGCATCTCTATGATATCGCGAATGTTAAGGGAGTTGCTGCTGATATCAGTCATTGCAACACCCCTTCGAAAGTTTTGGATTTCACAGGTGCTTCTGAGTTAGCAAATTGTTTGAAAGGTGTGGATGTTGTTGTTATCCCTGCTGGTGTTCCTAGGAAGCCCGGCATGACTCGTGACGACCTTTTCAACATCAATGCTGGTATAGTCAGGGACTTGGTCACTGCCGTTGCAGATAATTGCCCCAATGCTTTTATTCATATTATCAGTAATCCAGTGAACTCTACAGTTCCTATTGCCGCTGAAATTCTAAAACAGAAGGGTGTCTATGATCCTAAGAAGCTCTTTGGTGTTTCTACACTTGATGTTGTGAGGGCAAACACATTTGTTGCTCAGAAGAAGAACCTGAGGTTGATTGATGTGGATGTTCCAGTTGTTGGAGGTCATGCCGGGATTACAATTCTTCCTCTCTTGTCAAAGACAAAACCCTCAGTGAGTTTCACTGATGAAGAAATTGAGGAGCTAACTGTCAGGATTCAAAACGCTGGAACTGAGGTTGTTGAGGCGAAGGCTGGTGCAGGGTCTGCTACTTTGTCAATGGCTTATGCAGCAGCAAGATTTGTTGAATCGTCTCTTCGTGCACTTGACGGAGACGCTGATGTGTATGAGTGCTCATTCGTACAGTCAGATCTGACCGACCTTCCGTTTTTTGCTTCAAGGGTGAAGATTGGTAGGAAAGGAGTCGAGGCTTTAATTCCAACTGATCTCCAAGGATTGAGTGAGTATGAGCAGAAGGCTTTGGAAGCACTTAAGCCAGAACTTAAGGCCAGCATCGAGAAGGGGGTTGCTTTTGCTCAAAAGCAAACTGTTACTGCTTAG
Protein sequence

>MS.gene58427.t1

MAAAASATFTIGTTQTGRSLPQSSPFGLKFNSQVNFNTFSGLKAMPSLRCDSESSFSGKETCAALRATFAPKAQKENRNLTRNLQPQASYKVAVLGAAGGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSKVLDFTGASELANCLKGVDVVVIPAGVPRKPGMTRDDLFNINAGIVRDLVTAVADNCPNAFIHIISNPVNSTVPIAAEILKQKGVYDPKKLFGVSTLDVVRANTFVAQKKNLRLIDVDVPVVGGHAGITILPLLSKTKPSVSFTDEEIEELTVRIQNAGTEVVEAKAGAGSATLSMAYAAARFVESSLRALDGDADVYECSFVQSDLTDLPFFASRVKIGRKGVEALIPTDLQGLSEYEQKALEALKPELKASIEKGVAFAQKQTVTA