AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58556

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58556.t1 MTR_5g037080 95.556 315 13 1 1 315 1 314 0.0 506
MS.gene58556.t1 MTR_4g100630 49.020 204 65 5 3 187 1 184 2.47e-56 185
MS.gene58556.t1 MTR_2g100930 49.275 207 59 6 3 182 1 188 4.88e-54 180
MS.gene58556.t1 MTR_4g107230 56.923 195 51 7 29 208 13 189 3.13e-48 163
MS.gene58556.t1 MTR_6g092540 47.727 176 67 5 3 167 1 162 1.67e-46 159
MS.gene58556.t1 MTR_8g101650 41.748 206 84 7 5 187 4 196 1.95e-39 140
MS.gene58556.t1 MTR_0036s0260 82.353 68 12 0 102 169 28 95 1.80e-34 125
MS.gene58556.t1 MTR_3g462790 68.966 87 25 1 91 175 134 220 6.21e-34 127
MS.gene58556.t1 MTR_2g090305 80.882 68 13 0 102 169 28 95 1.41e-33 122
MS.gene58556.t1 MTR_4g111975 72.603 73 20 0 96 168 132 204 3.50e-33 124
MS.gene58556.t1 MTR_5g069710 70.513 78 23 0 96 173 126 203 2.36e-32 122
MS.gene58556.t1 MTR_3g104370 41.954 174 77 6 1 166 1 158 8.96e-32 119
MS.gene58556.t1 MTR_7g067080 67.949 78 25 0 96 173 134 211 5.12e-31 119
MS.gene58556.t1 MTR_1g111830 68.421 76 24 0 93 168 115 190 1.12e-30 116
MS.gene58556.t1 MTR_5g081860 50.847 118 53 2 89 204 112 226 1.01e-29 114
MS.gene58556.t1 MTR_8g063600 60.759 79 29 1 91 167 95 173 4.50e-25 102
MS.gene58556.t1 MTR_5g088010 33.939 165 97 6 96 252 54 214 2.18e-20 89.0
MS.gene58556.t1 MTR_4g015130 56.923 65 28 0 112 176 3 67 3.60e-20 83.6
MS.gene58556.t1 MTR_1g048660 55.224 67 30 0 103 169 13 79 8.84e-20 82.8
MS.gene58556.t1 MTR_5g027570 50.617 81 34 2 95 169 81 161 1.13e-19 86.7
MS.gene58556.t1 MTR_8g063870 56.061 66 27 1 104 167 94 159 4.73e-19 85.5
MS.gene58556.t1 MTR_5g088060 43.750 96 48 2 104 193 274 369 9.72e-19 86.7
MS.gene58556.t1 MTR_5g488160 43.750 96 48 2 104 193 274 369 9.72e-19 86.7
MS.gene58556.t1 MTR_5g027550 63.333 60 20 1 95 154 70 127 2.04e-18 82.8
MS.gene58556.t1 MTR_1g033620 72.549 51 14 0 118 168 21 71 2.14e-18 83.2
MS.gene58556.t1 MTR_5g075760 45.455 77 39 2 94 168 97 172 7.57e-13 67.0
MS.gene58556.t1 MTR_1g083180 48.571 70 32 2 104 170 63 131 1.10e-12 65.9
MS.gene58556.t1 MTR_5g075790 49.254 67 31 2 104 168 115 180 4.50e-12 65.1
MS.gene58556.t1 MTR_3g070110 45.833 72 32 1 104 168 97 168 6.65e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58556.t1 AT1G70000 48.923 325 90 11 3 315 1 261 1.24e-73 228
MS.gene58556.t1 AT1G70000 48.923 325 90 11 3 315 1 261 1.24e-73 228
MS.gene58556.t1 AT5G47390 52.717 184 53 3 3 171 1 165 7.37e-57 188
MS.gene58556.t1 AT5G61620 45.856 181 83 5 1 173 6 179 4.93e-46 158
MS.gene58556.t1 AT5G56840 47.727 176 81 5 3 176 1 167 6.49e-44 150
MS.gene58556.t1 AT3G16350 43.723 231 65 5 3 182 1 217 7.38e-44 154
MS.gene58556.t1 AT1G74840 53.368 193 55 5 20 196 15 188 4.66e-43 148
MS.gene58556.t1 AT1G74840 55.932 177 45 4 20 182 15 172 9.10e-43 148
MS.gene58556.t1 AT1G19000 52.308 195 52 6 20 192 13 188 1.64e-42 148
MS.gene58556.t1 AT1G19000 52.308 195 52 6 20 192 13 188 1.64e-42 148
MS.gene58556.t1 AT5G58900 64.773 88 29 1 92 177 129 216 2.29e-33 124
MS.gene58556.t1 AT1G49010 73.973 73 19 0 96 168 128 200 3.34e-33 124
MS.gene58556.t1 AT5G08520 69.863 73 22 0 96 168 112 184 8.52e-32 120
MS.gene58556.t1 AT2G38090 69.333 75 23 0 97 171 136 210 9.35e-32 120
MS.gene58556.t1 AT5G04760 73.611 72 19 0 97 168 94 165 2.76e-31 117
MS.gene58556.t1 AT5G01200 67.123 73 24 0 96 168 140 212 1.40e-29 114
MS.gene58556.t1 AT3G11280 60.976 82 32 0 91 172 116 197 1.36e-28 111
MS.gene58556.t1 AT3G11280 60.976 82 32 0 91 172 116 197 1.36e-28 111
MS.gene58556.t1 AT5G05790 59.756 82 33 0 91 172 120 201 1.02e-27 109
MS.gene58556.t1 AT5G05790 59.756 82 33 0 91 172 120 201 1.02e-27 109
MS.gene58556.t1 AT5G23650 56.579 76 33 0 92 167 110 185 4.09e-22 95.1
MS.gene58556.t1 AT3G10580 57.143 70 28 1 98 167 91 158 1.32e-21 92.4
MS.gene58556.t1 AT3G10580 57.143 70 28 1 98 167 91 158 1.95e-21 92.4
MS.gene58556.t1 AT4G09450 51.685 89 39 2 83 167 68 156 2.08e-21 90.5
MS.gene58556.t1 AT3G10590 50.617 81 37 2 92 170 99 178 7.03e-19 83.6

Find 106 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATTGGTGTTGGCCTAAT+TGG 0.245624 5.3:-25453928 None:intergenic
GTGGTGGTGGTGGAGGTGTT+GGG 0.249956 5.3:-25452521 None:intergenic
GGTGGTGGTGGTGGAGGTGT+TGG 0.254614 5.3:-25452522 None:intergenic
ATCTCCGGCGGCGGCGATTC+TGG 0.255355 5.3:-25452938 None:intergenic
CTTCTTGTTGGGGTTGCAAC+AGG 0.297790 5.3:+25452812 MS.gene58556:CDS
GACGAATGGCTGCTACTTTC+CGG 0.303324 5.3:-25454072 None:intergenic
CTGACCTCGTTTGCGTTCTC+TGG 0.320215 5.3:-25452678 None:intergenic
TGGTGGTGGTGGAGGTGTTG+GGG 0.324638 5.3:-25452520 None:intergenic
GAACACAAGCTCTTCTTGTT+GGG 0.336533 5.3:+25452801 MS.gene58556:CDS
GATGGAACCTTCAACTATAA+TGG 0.360343 5.3:+25453747 MS.gene58556:CDS
ATTGTTATTGGTGGTGGTGG+TGG 0.365994 5.3:-25452534 None:intergenic
AAAGAGTGGCCAAGCCAATT+AGG 0.366908 5.3:+25453914 MS.gene58556:CDS
CTCATTACCGGCAATGTTAT+CGG 0.388012 5.3:-25453886 None:intergenic
ACTCTTTCAGCACTCATTAC+CGG 0.403021 5.3:-25453898 None:intergenic
GTGGTTGTTCATATTGAGAA+AGG 0.407804 5.3:-25452586 None:intergenic
AGGATTGTTATTGGTGGTGG+TGG 0.412317 5.3:-25452537 None:intergenic
TCATCTTCCATTATAGTTGA+AGG 0.430302 5.3:-25453754 None:intergenic
GTCGTCATCGGCTTCATCAT+CGG 0.450173 5.3:+25454092 MS.gene58556:CDS
AACGACGGTGGAGAAGAAAA+AGG 0.452054 5.3:+25452455 MS.gene58556:CDS
CACATTACGGCGGCATCCCC+GGG 0.455334 5.3:+25453839 MS.gene58556:CDS
TCACATTACGGCGGCATCCC+CGG 0.455435 5.3:+25453838 MS.gene58556:CDS
AACAGGTTGGTAAAGGTGAT+TGG 0.456244 5.3:+25452829 MS.gene58556:CDS
AGAACACAAGCTCTTCTTGT+TGG 0.458844 5.3:+25452800 MS.gene58556:CDS
ATGGCTGCAGGGAAGTATAA+TGG 0.461919 5.3:+25454126 MS.gene58556:CDS
TCTTGGTCCGATAACATTGC+CGG 0.478043 5.3:+25453879 MS.gene58556:CDS
GATGTCATCGGATACATAAC+CGG 0.484684 5.3:-25452636 None:intergenic
GAAAGGATTGTTATTGGTGG+TGG 0.486611 5.3:-25452540 None:intergenic
ACCCATCGGAAAAGGTGTCC+CGG 0.489443 5.3:-25453857 None:intergenic
GATACATAACCGGCATCCGC+GGG 0.489451 5.3:-25452626 None:intergenic
TGGTTGGTGAAGGTGTTCCT+TGG 0.492029 5.3:+25452769 MS.gene58556:intron
CCCATCGGAAAAGGTGTCCC+GGG 0.492080 5.3:-25453856 None:intergenic
GTCATTGAAGCTGCAAACTT+CGG 0.497600 5.3:+25454029 MS.gene58556:CDS
TCTGAAAGGATTGTTATTGG+TGG 0.500851 5.3:-25452543 None:intergenic
AGATTAGCCATCTTAGAAGA+AGG 0.500963 5.3:-25453955 None:intergenic
TTGTGTTCTTCCTCTGTCCA+AGG 0.501291 5.3:-25452786 None:intergenic
GTTGGGGTTGTTGTTTCCTC+CGG 0.501389 5.3:-25452504 None:intergenic
TGGTGTTGGCCTAATTGGCT+TGG 0.501496 5.3:-25453923 None:intergenic
TGACAATGGAAAAGGCTCAA+TGG 0.502601 5.3:-25454010 None:intergenic
CTAGAAACTTTGTGAAAACA+AGG 0.504909 5.3:+25452862 MS.gene58556:CDS
GATGACATCGTTCACGCCTC+TGG 0.506341 5.3:+25452650 MS.gene58556:CDS
AGCTTCAATGACAATGGAAA+AGG 0.506440 5.3:-25454018 None:intergenic
GCATATCCATCCTCACATTA+CGG 0.507263 5.3:+25453826 MS.gene58556:CDS
ATAACCGGCATCCGCGGGGT+TGG 0.510315 5.3:-25452621 None:intergenic
GGACCAAGACCCATCGGAAA+AGG 0.510867 5.3:-25453865 None:intergenic
GGGTTGCAACAGGTTGGTAA+AGG 0.511124 5.3:+25452822 MS.gene58556:CDS
CGTTGCATGTTCTCGAGTTG+TGG 0.513462 5.3:-25452436 None:intergenic
GCTGCCTCCTTCTTCTAAGA+TGG 0.520621 5.3:+25453948 MS.gene58556:CDS
TAGCAGCCATTCGTCGTCAT+CGG 0.522877 5.3:+25454080 MS.gene58556:CDS
TGTGAGGATGGATATGCGGC+GGG 0.524024 5.3:-25453820 None:intergenic
AACACAAGCTCTTCTTGTTG+GGG 0.525017 5.3:+25452802 MS.gene58556:CDS
GGTGGTAATATCAAAGAGAC+TGG 0.526759 5.3:-25452961 None:intergenic
TTAGAAGAAGGAGGCAGCAT+TGG 0.530338 5.3:-25453943 None:intergenic
AAGAATAAATATTACCGTAT+CGG 0.530503 5.3:-25452982 None:intergenic
TGAGGATGGATATGCGGCGG+GGG 0.530821 5.3:-25453818 None:intergenic
GATTCCAACCCCGCGGATGC+CGG 0.538280 5.3:+25452617 MS.gene58556:CDS
ATAACCAGAATCGCCGCCGC+CGG 0.542005 5.3:+25452934 MS.gene58556:CDS
AGAGACTGGATCTCCGGCGG+CGG 0.545278 5.3:-25452947 None:intergenic
GCAGGGAAGTATAATGGTGG+GGG 0.547007 5.3:+25454132 MS.gene58556:CDS
TATCAAAGAGACTGGATCTC+CGG 0.548838 5.3:-25452953 None:intergenic
CTTTGATATTACCACCGATA+CGG 0.550867 5.3:+25452968 MS.gene58556:CDS
TTGTTCATATTGAGAAAGGT+TGG 0.551197 5.3:-25452582 None:intergenic
GGGTTGGAATCTTGTGGAGG+TGG 0.557505 5.3:-25452605 None:intergenic
TTGTTGGGGTTGCAACAGGT+TGG 0.558381 5.3:+25452816 MS.gene58556:CDS
GCTGCAAACTTCGGATCCAT+CGG 0.562043 5.3:+25454038 MS.gene58556:CDS
TCCGGTGAATGATCTTCCGA+TGG 0.563931 5.3:-25454054 None:intergenic
ATTATACTTCCCTGCAGCCA+TGG 0.565515 5.3:-25454124 None:intergenic
TCCGCGGGGTTGGAATCTTG+TGG 0.576478 5.3:-25452611 None:intergenic
GAAGGAGGCAGCATTGGTGT+TGG 0.579116 5.3:-25453937 None:intergenic
CTGCAGGGAAGTATAATGGT+GGG 0.579931 5.3:+25454130 MS.gene58556:CDS
TGCAGGGAAGTATAATGGTG+GGG 0.579949 5.3:+25454131 MS.gene58556:CDS
GTTTGCAGCTTCAATGACAA+TGG 0.579978 5.3:-25454024 None:intergenic
AATAAATATTACCGTATCGG+TGG 0.581515 5.3:-25452979 None:intergenic
GCATGACTTGCAACCTGAGT+TGG 0.584887 5.3:-25452888 None:intergenic
TGCTCACAGTGCGGCAACAA+CGG 0.587587 5.3:+25452413 MS.gene58556:CDS
GAAAACAAGGACACCAACTC+AGG 0.592034 5.3:+25452875 MS.gene58556:CDS
GGGGATGCCGCCGTAATGTG+AGG 0.595124 5.3:-25453836 None:intergenic
ATGCCGCCGTAATGTGAGGA+TGG 0.597354 5.3:-25453832 None:intergenic
GGCGGCGATTCTGGTTATGA+CGG 0.597516 5.3:-25452929 None:intergenic
GCGTTCTCTGGATCGACCAG+AGG 0.617091 5.3:-25452666 None:intergenic
GTTATCGGACCAAGACCCAT+CGG 0.618672 5.3:-25453871 None:intergenic
ATGAAGCCGATGACGACGAA+TGG 0.619919 5.3:-25454086 None:intergenic
TGAAGGTGTTCCTTGGACAG+AGG 0.622183 5.3:+25452776 MS.gene58556:intron
TCGAGAACATGCAACGACGG+TGG 0.622712 5.3:+25452443 MS.gene58556:CDS
GGATACATAACCGGCATCCG+CGG 0.626209 5.3:-25452627 None:intergenic
TCCATCGGAAGATCATTCAC+CGG 0.629452 5.3:+25454053 MS.gene58556:CDS
AGAGGCGTGAACGATGTCAT+CGG 0.629917 5.3:-25452648 None:intergenic
TTTGGTGTTCGACTCACCGG+AGG 0.638984 5.3:+25452488 MS.gene58556:CDS
GATTCTGGTTATGACGGCGG+AGG 0.639164 5.3:-25452923 None:intergenic
ATGTGAGGATGGATATGCGG+CGG 0.648268 5.3:-25453821 None:intergenic
AACTCGAGAACATGCAACGA+CGG 0.649079 5.3:+25452440 MS.gene58556:CDS
GCTGCAGGGAAGTATAATGG+TGG 0.654189 5.3:+25454129 MS.gene58556:CDS
GGTAATGAGTGCTGAAAGAG+TGG 0.656043 5.3:+25453900 MS.gene58556:CDS
CGATCCAGAGAACGCAAACG+AGG 0.663587 5.3:+25452674 MS.gene58556:CDS
GTTGGTAAAGGTGATTGGAG+AGG 0.666734 5.3:+25452834 MS.gene58556:CDS
TTAGCCATCTTAGAAGAAGG+AGG 0.666938 5.3:-25453952 None:intergenic
GGCGATTCTGGTTATGACGG+CGG 0.668821 5.3:-25452926 None:intergenic
GCGGGGTTGGAATCTTGTGG+AGG 0.674293 5.3:-25452608 None:intergenic
CCATCGGAAAAGGTGTCCCG+GGG 0.680456 5.3:-25453855 None:intergenic
CAAAGAGACTGGATCTCCGG+CGG 0.680570 5.3:-25452950 None:intergenic
GTGAGGATGGATATGCGGCG+GGG 0.685316 5.3:-25453819 None:intergenic
TCTCGCACGTGCTCACAGTG+CGG 0.694033 5.3:+25452404 MS.gene58556:CDS
GTAATGTGAGGATGGATATG+CGG 0.700958 5.3:-25453824 None:intergenic
TCAATTTCAGCAAGAAACAG+TGG 0.701868 5.3:+25453777 MS.gene58556:CDS
TCCACAAGATTCCAACCCCG+CGG 0.717102 5.3:+25452610 MS.gene58556:CDS
ATACATAACCGGCATCCGCG+GGG 0.725166 5.3:-25452625 None:intergenic
TATCCATCCTCACATTACGG+CGG 0.751877 5.3:+25453829 MS.gene58556:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATACGAAAATATTTA+AGG - chr5.3:25453098-25453117 None:intergenic 10.0%
!! TAAAATCAAATTATTAGAAA+AGG - chr5.3:25453051-25453070 None:intergenic 10.0%
!!! CAATTTTGATTATTTATTTT+AGG + chr5.3:25453723-25453742 MS.gene58556:intron 10.0%
!!! TATATTAATGTTTTTTGTTT+TGG + chr5.3:25452749-25452768 MS.gene58556:intron 10.0%
!! AAGAATAAATATTACCGTAT+CGG - chr5.3:25452985-25453004 None:intergenic 20.0%
!! CGAAAATATTTAAGGAAATT+TGG - chr5.3:25453090-25453109 None:intergenic 20.0%
!! GTCTCAAAAAAAATTATCTA+CGG + chr5.3:25453619-25453638 MS.gene58556:intron 20.0%
!!! GTAGATAATTTTTTTTGAGA+CGG - chr5.3:25453620-25453639 None:intergenic 20.0%
!!! TGATTATTTATTTTAGGTGA+TGG + chr5.3:25453729-25453748 MS.gene58556:intron 20.0%
!!! TTAATGTTTTTTGTTTTGGT+TGG + chr5.3:25452753-25452772 MS.gene58556:intron 20.0%
!!! TTTTCTGAAAGGATTGTTAT+TGG - chr5.3:25452549-25452568 None:intergenic 25.0%
AATAAATATTACCGTATCGG+TGG - chr5.3:25452982-25453001 None:intergenic 30.0%
CTAGAAACTTTGTGAAAACA+AGG + chr5.3:25452862-25452881 MS.gene58556:CDS 30.0%
TCATCTTCCATTATAGTTGA+AGG - chr5.3:25453757-25453776 None:intergenic 30.0%
TTGTTCATATTGAGAAAGGT+TGG - chr5.3:25452585-25452604 None:intergenic 30.0%
!!! GAAAAAGGCATCATGATTTT+TGG + chr5.3:25452470-25452489 MS.gene58556:CDS 30.0%
!!! GATAATTTTTTTTGAGACGG+AGG - chr5.3:25453617-25453636 None:intergenic 30.0%
!!! TTTTTTGTTTTGGTTGGTGA+AGG + chr5.3:25452759-25452778 MS.gene58556:intron 30.0%
AGCTTCAATGACAATGGAAA+AGG - chr5.3:25454021-25454040 None:intergenic 35.0%
CTTTGATATTACCACCGATA+CGG + chr5.3:25452968-25452987 MS.gene58556:CDS 35.0%
GATGGAACCTTCAACTATAA+TGG + chr5.3:25453747-25453766 MS.gene58556:CDS 35.0%
GTGGTTGTTCATATTGAGAA+AGG - chr5.3:25452589-25452608 None:intergenic 35.0%
TCAATTTCAGCAAGAAACAG+TGG + chr5.3:25453777-25453796 MS.gene58556:CDS 35.0%
! AGATTAGCCATCTTAGAAGA+AGG - chr5.3:25453958-25453977 None:intergenic 35.0%
! TCTGAAAGGATTGTTATTGG+TGG - chr5.3:25452546-25452565 None:intergenic 35.0%
!!! ATCATCGGCTTTTAAAACCA+TGG + chr5.3:25454107-25454126 MS.gene58556:CDS 35.0%
!!! ATGCTAGCACTTTTTCTGAA+AGG - chr5.3:25452560-25452579 None:intergenic 35.0%
AACACAAGCTCTTCTTGTTG+GGG + chr5.3:25452802-25452821 MS.gene58556:CDS 40.0%
ACTCTTTCAGCACTCATTAC+CGG - chr5.3:25453901-25453920 None:intergenic 40.0%
AGAACACAAGCTCTTCTTGT+TGG + chr5.3:25452800-25452819 MS.gene58556:CDS 40.0%
CTCATTACCGGCAATGTTAT+CGG - chr5.3:25453889-25453908 None:intergenic 40.0%
GAACACAAGCTCTTCTTGTT+GGG + chr5.3:25452801-25452820 MS.gene58556:CDS 40.0%
GATGTCATCGGATACATAAC+CGG - chr5.3:25452639-25452658 None:intergenic 40.0%
GCATATCCATCCTCACATTA+CGG + chr5.3:25453826-25453845 MS.gene58556:CDS 40.0%
GGTGGTAATATCAAAGAGAC+TGG - chr5.3:25452964-25452983 None:intergenic 40.0%
GTAATGTGAGGATGGATATG+CGG - chr5.3:25453827-25453846 None:intergenic 40.0%
GTCATTGAAGCTGCAAACTT+CGG + chr5.3:25454029-25454048 MS.gene58556:CDS 40.0%
GTTTGCAGCTTCAATGACAA+TGG - chr5.3:25454027-25454046 None:intergenic 40.0%
TATCAAAGAGACTGGATCTC+CGG - chr5.3:25452956-25452975 None:intergenic 40.0%
TGACAATGGAAAAGGCTCAA+TGG - chr5.3:25454013-25454032 None:intergenic 40.0%
TTAGCCATCTTAGAAGAAGG+AGG - chr5.3:25453955-25453974 None:intergenic 40.0%
! GAAAGGATTGTTATTGGTGG+TGG - chr5.3:25452543-25452562 None:intergenic 40.0%
!! AACAGGTTGGTAAAGGTGAT+TGG + chr5.3:25452829-25452848 MS.gene58556:CDS 40.0%
!!! ATTTTTGGTGTTCGACTCAC+CGG + chr5.3:25452485-25452504 MS.gene58556:CDS 40.0%
!!! CTTTTAAAACCATGGCTGCA+GGG + chr5.3:25454115-25454134 MS.gene58556:CDS 40.0%
AAAGAGTGGCCAAGCCAATT+AGG + chr5.3:25453914-25453933 MS.gene58556:CDS 45.0%
AACGACGGTGGAGAAGAAAA+AGG + chr5.3:25452455-25452474 MS.gene58556:CDS 45.0%
AACTCGAGAACATGCAACGA+CGG + chr5.3:25452440-25452459 MS.gene58556:CDS 45.0%
ATTATACTTCCCTGCAGCCA+TGG - chr5.3:25454127-25454146 None:intergenic 45.0%
GAAAACAAGGACACCAACTC+AGG + chr5.3:25452875-25452894 MS.gene58556:CDS 45.0%
TATCCATCCTCACATTACGG+CGG + chr5.3:25453829-25453848 MS.gene58556:CDS 45.0%
TCCATCGGAAGATCATTCAC+CGG + chr5.3:25454053-25454072 MS.gene58556:CDS 45.0%
TCTTGGTCCGATAACATTGC+CGG + chr5.3:25453879-25453898 MS.gene58556:CDS 45.0%
TTAGAAGAAGGAGGCAGCAT+TGG - chr5.3:25453946-25453965 None:intergenic 45.0%
TTGTGTTCTTCCTCTGTCCA+AGG - chr5.3:25452789-25452808 None:intergenic 45.0%
! ATGGCTGCAGGGAAGTATAA+TGG + chr5.3:25454126-25454145 MS.gene58556:CDS 45.0%
! CTGCAGGGAAGTATAATGGT+GGG + chr5.3:25454130-25454149 MS.gene58556:CDS 45.0%
! GTTGGTAAAGGTGATTGGAG+AGG + chr5.3:25452834-25452853 MS.gene58556:CDS 45.0%
! TGCAGGGAAGTATAATGGTG+GGG + chr5.3:25454131-25454150 MS.gene58556:CDS 45.0%
!! AGCATTGGTGTTGGCCTAAT+TGG - chr5.3:25453931-25453950 None:intergenic 45.0%
!! AGGATTGTTATTGGTGGTGG+TGG - chr5.3:25452540-25452559 None:intergenic 45.0%
!! ATTGTTATTGGTGGTGGTGG+TGG - chr5.3:25452537-25452556 None:intergenic 45.0%
!! GGTAATGAGTGCTGAAAGAG+TGG + chr5.3:25453900-25453919 MS.gene58556:CDS 45.0%
!!! GCTTTTAAAACCATGGCTGC+AGG + chr5.3:25454114-25454133 MS.gene58556:CDS 45.0%
ATGAAGCCGATGACGACGAA+TGG - chr5.3:25454089-25454108 None:intergenic 50.0%
ATGTGAGGATGGATATGCGG+CGG - chr5.3:25453824-25453843 None:intergenic 50.0%
CTTCTTGTTGGGGTTGCAAC+AGG + chr5.3:25452812-25452831 MS.gene58556:CDS 50.0%
GCTGCAAACTTCGGATCCAT+CGG + chr5.3:25454038-25454057 MS.gene58556:CDS 50.0%
GTTATCGGACCAAGACCCAT+CGG - chr5.3:25453874-25453893 None:intergenic 50.0%
TAGCAGCCATTCGTCGTCAT+CGG + chr5.3:25454080-25454099 MS.gene58556:CDS 50.0%
TCCGGTGAATGATCTTCCGA+TGG - chr5.3:25454057-25454076 None:intergenic 50.0%
TTGTTGGGGTTGCAACAGGT+TGG + chr5.3:25452816-25452835 MS.gene58556:CDS 50.0%
! ACACCTTTTCCGATGGGTCT+TGG + chr5.3:25453862-25453881 MS.gene58556:CDS 50.0%
! AGAGGCGTGAACGATGTCAT+CGG - chr5.3:25452651-25452670 None:intergenic 50.0%
! CGTTGCATGTTCTCGAGTTG+TGG - chr5.3:25452439-25452458 None:intergenic 50.0%
! GAAGTATAATGGTGGGGGTG+GGG + chr5.3:25454137-25454156 MS.gene58556:CDS 50.0%
! GCAGGGAAGTATAATGGTGG+GGG + chr5.3:25454132-25454151 MS.gene58556:CDS 50.0%
! GCATGACTTGCAACCTGAGT+TGG - chr5.3:25452891-25452910 None:intergenic 50.0%
! GCTGCAGGGAAGTATAATGG+TGG + chr5.3:25454129-25454148 MS.gene58556:CDS 50.0%
! GCTGCCTCCTTCTTCTAAGA+TGG + chr5.3:25453948-25453967 MS.gene58556:CDS 50.0%
! GGAAGTATAATGGTGGGGGT+GGG + chr5.3:25454136-25454155 MS.gene58556:CDS 50.0%
!! GACGAATGGCTGCTACTTTC+CGG - chr5.3:25454075-25454094 None:intergenic 50.0%
!! GGGTTGCAACAGGTTGGTAA+AGG + chr5.3:25452822-25452841 MS.gene58556:CDS 50.0%
!! GTCGTCATCGGCTTCATCAT+CGG + chr5.3:25454092-25454111 MS.gene58556:CDS 50.0%
!! GTTGGGGTTGTTGTTTCCTC+CGG - chr5.3:25452507-25452526 None:intergenic 50.0%
!! TGAAGGTGTTCCTTGGACAG+AGG + chr5.3:25452776-25452795 MS.gene58556:intron 50.0%
!! TGGTGTTGGCCTAATTGGCT+TGG - chr5.3:25453926-25453945 None:intergenic 50.0%
!! TGGTTGGTGAAGGTGTTCCT+TGG + chr5.3:25452769-25452788 MS.gene58556:intron 50.0%
ATACATAACCGGCATCCGCG+GGG - chr5.3:25452628-25452647 None:intergenic 55.0%
ATGCCGCCGTAATGTGAGGA+TGG - chr5.3:25453835-25453854 None:intergenic 55.0%
CAAAGAGACTGGATCTCCGG+CGG - chr5.3:25452953-25452972 None:intergenic 55.0%
CGATCCAGAGAACGCAAACG+AGG + chr5.3:25452674-25452693 MS.gene58556:CDS 55.0%
CTGACCTCGTTTGCGTTCTC+TGG - chr5.3:25452681-25452700 None:intergenic 55.0%
GATACATAACCGGCATCCGC+GGG - chr5.3:25452629-25452648 None:intergenic 55.0%
GATGACATCGTTCACGCCTC+TGG + chr5.3:25452650-25452669 MS.gene58556:CDS 55.0%
GGACCAAGACCCATCGGAAA+AGG - chr5.3:25453868-25453887 None:intergenic 55.0%
GGATACATAACCGGCATCCG+CGG - chr5.3:25452630-25452649 None:intergenic 55.0%
GGGTTGGAATCTTGTGGAGG+TGG - chr5.3:25452608-25452627 None:intergenic 55.0%
TCCACAAGATTCCAACCCCG+CGG + chr5.3:25452610-25452629 MS.gene58556:CDS 55.0%
TCGAGAACATGCAACGACGG+TGG + chr5.3:25452443-25452462 MS.gene58556:CDS 55.0%
TGCTCACAGTGCGGCAACAA+CGG + chr5.3:25452413-25452432 MS.gene58556:CDS 55.0%
TGTGAGGATGGATATGCGGC+GGG - chr5.3:25453823-25453842 None:intergenic 55.0%
! GATTCTGGTTATGACGGCGG+AGG - chr5.3:25452926-25452945 None:intergenic 55.0%
! GGCGATTCTGGTTATGACGG+CGG - chr5.3:25452929-25452948 None:intergenic 55.0%
! GGCGGCGATTCTGGTTATGA+CGG - chr5.3:25452932-25452951 None:intergenic 55.0%
! GGGAAGTATAATGGTGGGGG+TGG + chr5.3:25454135-25454154 MS.gene58556:CDS 55.0%
!! ACCCATCGGAAAAGGTGTCC+CGG - chr5.3:25453860-25453879 None:intergenic 55.0%
!! GAAGGAGGCAGCATTGGTGT+TGG - chr5.3:25453940-25453959 None:intergenic 55.0%
!! GTTATTGGTGGTGGTGGTGG+TGG - chr5.3:25452534-25452553 None:intergenic 55.0%
!! TTTGGTGTTCGACTCACCGG+AGG + chr5.3:25452488-25452507 MS.gene58556:CDS 55.0%
ATAACCAGAATCGCCGCCGC+CGG + chr5.3:25452934-25452953 MS.gene58556:CDS 60.0%
GCGGGGTTGGAATCTTGTGG+AGG - chr5.3:25452611-25452630 None:intergenic 60.0%
GCGTTCTCTGGATCGACCAG+AGG - chr5.3:25452669-25452688 None:intergenic 60.0%
GTGAGGATGGATATGCGGCG+GGG - chr5.3:25453822-25453841 None:intergenic 60.0%
TCACATTACGGCGGCATCCC+CGG + chr5.3:25453838-25453857 MS.gene58556:CDS 60.0%
TCCGCGGGGTTGGAATCTTG+TGG - chr5.3:25452614-25452633 None:intergenic 60.0%
TCTCGCACGTGCTCACAGTG+CGG + chr5.3:25452404-25452423 MS.gene58556:CDS 60.0%
TGAGGATGGATATGCGGCGG+GGG - chr5.3:25453821-25453840 None:intergenic 60.0%
! CCCGGGACACCTTTTCCGAT+GGG + chr5.3:25453856-25453875 MS.gene58556:CDS 60.0%
!! ATTGGTGGTGGTGGTGGTGG+AGG - chr5.3:25452531-25452550 None:intergenic 60.0%
!! CCATCGGAAAAGGTGTCCCG+GGG - chr5.3:25453858-25453877 None:intergenic 60.0%
!! CCCATCGGAAAAGGTGTCCC+GGG - chr5.3:25453859-25453878 None:intergenic 60.0%
!! GTGGTGGTGGTGGAGGTGTT+GGG - chr5.3:25452524-25452543 None:intergenic 60.0%
!! TGGTGGTGGTGGAGGTGTTG+GGG - chr5.3:25452523-25452542 None:intergenic 60.0%
AGAGACTGGATCTCCGGCGG+CGG - chr5.3:25452950-25452969 None:intergenic 65.0%
ATAACCGGCATCCGCGGGGT+TGG - chr5.3:25452624-25452643 None:intergenic 65.0%
CACATTACGGCGGCATCCCC+GGG + chr5.3:25453839-25453858 MS.gene58556:CDS 65.0%
GATGGATATGCGGCGGGGGT+TGG - chr5.3:25453817-25453836 None:intergenic 65.0%
GATTCCAACCCCGCGGATGC+CGG + chr5.3:25452617-25452636 MS.gene58556:CDS 65.0%
GGGGATGCCGCCGTAATGTG+AGG - chr5.3:25453839-25453858 None:intergenic 65.0%
! CCCCGGGACACCTTTTCCGA+TGG + chr5.3:25453855-25453874 MS.gene58556:CDS 65.0%
!! GGTGGTGGTGGTGGAGGTGT+TGG - chr5.3:25452525-25452544 None:intergenic 65.0%
ATCTCCGGCGGCGGCGATTC+TGG - chr5.3:25452941-25452960 None:intergenic 70.0%
GGATATGCGGCGGGGGTTGG+TGG - chr5.3:25453814-25453833 None:intergenic 70.0%
!! TATGCGGCGGGGGTTGGTGG+TGG - chr5.3:25453811-25453830 None:intergenic 70.0%
!! GCGGGGGTTGGTGGTGGCAG+TGG - chr5.3:25453805-25453824 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 25452395 25454182 25452395 ID=MS.gene58556
chr5.3 mRNA 25452395 25454182 25452395 ID=MS.gene58556.t1;Parent=MS.gene58556
chr5.3 exon 25452395 25452695 25452395 ID=MS.gene58556.t1.exon1;Parent=MS.gene58556.t1
chr5.3 CDS 25452395 25452695 25452395 ID=cds.MS.gene58556.t1;Parent=MS.gene58556.t1
chr5.3 exon 25452781 25452989 25452781 ID=MS.gene58556.t1.exon2;Parent=MS.gene58556.t1
chr5.3 CDS 25452781 25452989 25452781 ID=cds.MS.gene58556.t1;Parent=MS.gene58556.t1
chr5.3 exon 25453745 25454182 25453745 ID=MS.gene58556.t1.exon3;Parent=MS.gene58556.t1
chr5.3 CDS 25453745 25454182 25453745 ID=cds.MS.gene58556.t1;Parent=MS.gene58556.t1
Gene Sequence

>MS.gene58556

ATGTCTTTGTCTCGCACGTGCTCACAGTGCGGCAACAACGGCCACAACTCGAGAACATGCAACGACGGTGGAGAAGAAAAAGGCATCATGATTTTTGGTGTTCGACTCACCGGAGGAAACAACAACCCCAACACCTCCACCACCACCACCACCAATAACAATCCTTTCAGAAAAAGTGCTAGCATGACCAACCTTTCTCAATATGAACAACCACCTCCACAAGATTCCAACCCCGCGGATGCCGGTTATGTATCCGATGACATCGTTCACGCCTCTGGTCGATCCAGAGAACGCAAACGAGGTCAGTATTTAATCTAGATACATCAACTTTTGTTTGATATATTTTTGCTTATGTATATTAATGTTTTTTGTTTTGGTTGGTGAAGGTGTTCCTTGGACAGAGGAAGAACACAAGCTCTTCTTGTTGGGGTTGCAACAGGTTGGTAAAGGTGATTGGAGAGGAATTTCTAGAAACTTTGTGAAAACAAGGACACCAACTCAGGTTGCAAGTCATGCTCAGAAGTATTTCCTCCGCCGTCATAACCAGAATCGCCGCCGCCGGAGATCCAGTCTCTTTGATATTACCACCGATACGGTAATATTTATTCTTTGTTTTAATCTTCTTTTAATAACTTTTATATTTTTAACAATTTCCTTTTCTAATAATTTGATTTTATTAGTTTAGTCAAAAACCAAATTTCCTTAAATATTTTCGTATAATTTTATTTTTCAAAGTAATAATGATGATGATAAATAATTAATAATAATTTACTCAAAAATAATAATTAATAATAATGTATTTTTTATTCAAATATATATAATCTTCATTTATTTTATTATATAAATATTAATGATGATGATGATGATGATGATAAATAATTAATAATAATGTGATTTTTCTACAAATATATAATCATAATTTAGTTTATTATACTTATTATATAAATATTAGATTTAATTATATATTATTAGTGTAAAATATTATTACATTATGAACTAATTGTAATCATCATATATTAATTTTTTATCTTTTTATTTTAGTTATAATTAAATTATAAATTACATATATGTTTGACTGTGATTTTAAATCTATTGACAATGTATCAATTAAATCTATACATATTTTTAACACAAATAATAATGTGATTTGATTTGATACGAGTAAAAAAATAACAATTGCATGTTTTTTTTTTACTATTGTGCTGTAGTATTTTTTACTCCTCCGTCTCAAAAAAAATTATCTACGGACAAATATTTTTACAAACGTGACGATTATTATGATAAAGAGATAAAGAGATAGTACTGTTGTATTAAAATTATCATTAATGCAATTTTGATTATTTATTTTAGGTGATGGAACCTTCAACTATAATGGAAGATGATCAATTTCAGCAAGAAACAGTGGCGCCACTGCCACCACCAACCCCCGCCGCATATCCATCCTCACATTACGGCGGCATCCCCGGGACACCTTTTCCGATGGGTCTTGGTCCGATAACATTGCCGGTAATGAGTGCTGAAAGAGTGGCCAAGCCAATTAGGCCAACACCAATGCTGCCTCCTTCTTCTAAGATGGCTAATCTGAACTTGAAAGACAAAGCTTCTTCTTCTTCTTCCATTGAGCCTTTTCCATTGTCATTGAAGCTGCAAACTTCGGATCCATCGGAAGATCATTCACCGGAAAGTAGCAGCCATTCGTCGTCATCGGCTTCATCATCGGCTTTTAAAACCATGGCTGCAGGGAAGTATAATGGTGGGGGTGGGGATAGCATTATCAGTGTTGCTTGA
Protein sequence

>MS.gene58556.t1

MSLSRTCSQCGNNGHNSRTCNDGGEEKGIMIFGVRLTGGNNNPNTSTTTTTNNNPFRKSASMTNLSQYEQPPPQDSNPADAGYVSDDIVHASGRSRERKRGVPWTEEEHKLFLLGLQQVGKGDWRGISRNFVKTRTPTQVASHAQKYFLRRHNQNRRRRRSSLFDITTDTVMEPSTIMEDDQFQQETVAPLPPPTPAAYPSSHYGGIPGTPFPMGLGPITLPVMSAERVAKPIRPTPMLPPSSKMANLNLKDKASSSSSIEPFPLSLKLQTSDPSEDHSPESSSHSSSSASSSAFKTMAAGKYNGGGGDSIISVA