Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58910.t1 | KEH39385.1 | 63 | 81 | 19 | 1 | 19 | 88 | 375 | 455 | 1.00E-13 | 85.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58910.t1 | Q9LHB1 | 50.0 | 58 | 29 | 0 | 22 | 79 | 380 | 437 | 2.3e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58910.t1 | A0A072VMP6 | 63.0 | 81 | 19 | 1 | 19 | 88 | 375 | 455 | 7.4e-14 | 85.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05288 | MS.gene58910 | 0.92514 | 2.75E-90 | -1.69E-46 |
| MS.gene05289 | MS.gene58910 | 0.903568 | 3.03E-79 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58910.t1 | MTR_4g126200 | 66.667 | 51 | 17 | 0 | 38 | 88 | 3 | 53 | 1.45e-13 | 63.9 |
| MS.gene58910.t1 | MTR_2g095400 | 68.750 | 64 | 20 | 0 | 19 | 82 | 375 | 438 | 2.06e-12 | 61.2 |
| MS.gene58910.t1 | MTR_5g079940 | 50.000 | 62 | 31 | 0 | 19 | 80 | 859 | 920 | 3.52e-12 | 60.8 |
| MS.gene58910.t1 | MTR_2g097010 | 67.188 | 64 | 21 | 0 | 19 | 82 | 401 | 464 | 2.37e-11 | 58.2 |
| MS.gene58910.t1 | MTR_2g097020 | 60.870 | 46 | 18 | 0 | 38 | 83 | 409 | 454 | 7.00e-11 | 57.0 |
| MS.gene58910.t1 | MTR_3g067815 | 50.820 | 61 | 30 | 0 | 22 | 82 | 382 | 442 | 7.33e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58910.t1 | AT4G01180 | 49.180 | 61 | 31 | 0 | 16 | 76 | 277 | 337 | 5.36e-11 | 57.4 |
Find 13 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGATGCAAGACATGCTT+TGG | 0.365400 | 6.1:+71472555 | MS.gene58910:CDS |
| TGCAAGACATGCTTTGGAAT+TGG | 0.380086 | 6.1:+71472561 | MS.gene58910:CDS |
| AAAGAAGAAATGGCGGAAGC+TGG | 0.447708 | 6.1:+71472293 | None:intergenic |
| ATTGCATCACTTATCTCGTC+TGG | 0.458810 | 6.1:-71472338 | None:intergenic |
| TTGGATGTTGCAGAAAGAAA+AGG | 0.471032 | 6.1:+71472495 | MS.gene58910:intron |
| AAGAAGAAATGGCGGAAGCT+GGG | 0.487627 | 6.1:+71472294 | None:intergenic |
| TGCAATATCAGATCAACAAA+AGG | 0.494458 | 6.1:+71472355 | MS.gene58910:CDS |
| GCGTTTGGCTGAAGACCATA+AGG | 0.535802 | 6.1:+71472394 | MS.gene58910:CDS |
| ATATCAGATCAACAAAAGGC+TGG | 0.578847 | 6.1:+71472359 | MS.gene58910:CDS |
| AGAAGAAATGGCGGAAGCTG+GGG | 0.585508 | 6.1:+71472295 | None:intergenic |
| AGAAATGGCGGAAGCTGGGG+TGG | 0.586264 | 6.1:+71472298 | None:intergenic |
| TCACAAGAAAATGCATGACC+TGG | 0.631525 | 6.1:+71472525 | MS.gene58910:CDS |
| TTGGAGATAGAACAGCTGAA+AGG | 0.635079 | 6.1:+71472580 | MS.gene58910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAAAAAATGTTGATAAA+AGG | - | chr6.1:71472435-71472454 | None:intergenic | 10.0% |
| ! | AAAATCAAATGTGTACCTTA+TGG | - | chr6.1:71472412-71472431 | None:intergenic | 25.0% |
| !!! | TTATGTTTTGATGACTCTTT+TGG | + | chr6.1:71472472-71472491 | MS.gene58910:intron | 25.0% |
| AAGAGTCATCAAAACATAAG+TGG | - | chr6.1:71472471-71472490 | None:intergenic | 30.0% | |
| TGCAATATCAGATCAACAAA+AGG | + | chr6.1:71472355-71472374 | MS.gene58910:CDS | 30.0% | |
| TTTCCGTGTAATGAAAAAAC+TGG | + | chr6.1:71472606-71472625 | MS.gene58910:CDS | 30.0% | |
| ATATCAGATCAACAAAAGGC+TGG | + | chr6.1:71472359-71472378 | MS.gene58910:CDS | 35.0% | |
| TGGTGAAAAAATGATGCGTT+TGG | + | chr6.1:71472379-71472398 | MS.gene58910:CDS | 35.0% | |
| TTGGATGTTGCAGAAAGAAA+AGG | + | chr6.1:71472495-71472514 | MS.gene58910:intron | 35.0% | |
| !! | GTTTTGATGACTCTTTTGGT+TGG | + | chr6.1:71472476-71472495 | MS.gene58910:intron | 35.0% |
| !!! | CCTCCAGTTTTTTCATTACA+CGG | - | chr6.1:71472612-71472631 | None:intergenic | 35.0% |
| ACTTGATGCAAGACATGCTT+TGG | + | chr6.1:71472555-71472574 | MS.gene58910:CDS | 40.0% | |
| ATTGCATCACTTATCTCGTC+TGG | - | chr6.1:71472341-71472360 | None:intergenic | 40.0% | |
| CCGTGTAATGAAAAAACTGG+AGG | + | chr6.1:71472609-71472628 | MS.gene58910:CDS | 40.0% | |
| TCACAAGAAAATGCATGACC+TGG | + | chr6.1:71472525-71472544 | MS.gene58910:CDS | 40.0% | |
| TTGGAGATAGAACAGCTGAA+AGG | + | chr6.1:71472580-71472599 | MS.gene58910:CDS | 40.0% | |
| ! | TTGCATCAAGTCTTTTCACC+AGG | - | chr6.1:71472546-71472565 | None:intergenic | 40.0% |
| !! | TGCAAGACATGCTTTGGAAT+TGG | + | chr6.1:71472561-71472580 | MS.gene58910:CDS | 40.0% |
| GCGTTTGGCTGAAGACCATA+AGG | + | chr6.1:71472394-71472413 | MS.gene58910:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 71472302 | 71472660 | 71472302 | ID=MS.gene58910 |
| chr6.1 | mRNA | 71472302 | 71472660 | 71472302 | ID=MS.gene58910.t1;Parent=MS.gene58910 |
| chr6.1 | exon | 71472302 | 71472415 | 71472302 | ID=MS.gene58910.t1.exon1;Parent=MS.gene58910.t1 |
| chr6.1 | CDS | 71472302 | 71472415 | 71472302 | ID=cds.MS.gene58910.t1;Parent=MS.gene58910.t1 |
| chr6.1 | exon | 71472508 | 71472660 | 71472508 | ID=MS.gene58910.t1.exon2;Parent=MS.gene58910.t1 |
| chr6.1 | CDS | 71472508 | 71472660 | 71472508 | ID=cds.MS.gene58910.t1;Parent=MS.gene58910.t1 |
| Gene Sequence |
| Protein sequence |
