Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59078.t1 | XP_003609265.1 | 99.1 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 8.60E-47 | 196.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59078.t1 | Q02921 | 84.8 | 99 | 15 | 0 | 9 | 107 | 5 | 103 | 1.8e-37 | 156.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59078.t1 | G7JVA6 | 99.1 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 6.2e-47 | 196.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055157 | MS.gene59078 | 0.846775 | 1.76E-59 | -1.69E-46 |
| MS.gene056899 | MS.gene59078 | 0.801241 | 9.88E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59078.t1 | MTR_4g113820 | 99.065 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 1.75e-72 | 211 |
| MS.gene59078.t1 | MTR_1g007360 | 60.759 | 79 | 31 | 0 | 21 | 99 | 26 | 104 | 7.36e-29 | 101 |
| MS.gene59078.t1 | MTR_3g110390 | 53.086 | 81 | 38 | 0 | 21 | 101 | 27 | 107 | 1.56e-25 | 92.8 |
| MS.gene59078.t1 | MTR_3g110390 | 51.852 | 81 | 38 | 1 | 21 | 101 | 27 | 106 | 8.87e-23 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59078.t1 | AT5G25940 | 67.347 | 49 | 16 | 0 | 52 | 100 | 55 | 103 | 9.73e-18 | 72.8 |
Find 31 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACCAGCTTGGCTTATC+TGG | 0.217498 | 8.4:-14099795 | MS.gene59078:CDS |
| GAGCTTGAGCAGTGGGATTC+AGG | 0.269132 | 8.4:+14099379 | None:intergenic |
| TGCATTGGAGGGTCGGTTGA+TGG | 0.374033 | 8.4:+14099182 | None:intergenic |
| TGCATTTGTAATTCATGCAT+TGG | 0.411064 | 8.4:+14099167 | None:intergenic |
| CTAGTGTTAGGATGCTACCT+TGG | 0.442749 | 8.4:-14099413 | MS.gene59078:CDS |
| CAAGCTCTCATAATTTCTAC+AGG | 0.456647 | 8.4:-14099363 | MS.gene59078:intron |
| ATTAGCAGCAACATTTCTCT+TGG | 0.469394 | 8.4:+14099829 | None:intergenic |
| GCTTGGCCATGGCCAACCTC+TGG | 0.472159 | 8.4:+14099771 | None:intergenic |
| ACATGCAGCTGGCTAGTGTT+AGG | 0.477537 | 8.4:-14099425 | MS.gene59078:intron |
| GGCTTATCTGGACCAGAGGT+TGG | 0.484520 | 8.4:-14099783 | MS.gene59078:CDS |
| TCCTCTTGAGAGAACCAGCT+TGG | 0.495079 | 8.4:-14099804 | MS.gene59078:CDS |
| ATGAGAACAGCGCTTGGCCA+TGG | 0.500574 | 8.4:+14099760 | None:intergenic |
| AGTAGCTGACAAGACTGTAT+TGG | 0.503109 | 8.4:-14099223 | MS.gene59078:CDS |
| GGGTGTAATGGCTGGAGCTA+AGG | 0.508105 | 8.4:-14099642 | MS.gene59078:CDS |
| TTCAGGTTTGCTCTTGCCCA+AGG | 0.514820 | 8.4:+14099396 | None:intergenic |
| TAGTGTTAGGATGCTACCTT+GGG | 0.518544 | 8.4:-14099412 | MS.gene59078:CDS |
| ATGGCCAAGCGCTGTTCTCA+TGG | 0.525880 | 8.4:-14099758 | MS.gene59078:intron |
| TAATTCATGCATTGGAGGGT+CGG | 0.526783 | 8.4:+14099175 | None:intergenic |
| ATTATGAGAGCTTGAGCAGT+GGG | 0.542769 | 8.4:+14099372 | None:intergenic |
| CTGGTCCAGATAAGCCAAGC+TGG | 0.549243 | 8.4:+14099790 | None:intergenic |
| ATTTGTAATTCATGCATTGG+AGG | 0.558334 | 8.4:+14099170 | None:intergenic |
| AGGAGTAGTTACAGTTGGAA+TGG | 0.559635 | 8.4:+14099592 | None:intergenic |
| TAAACAGAGGGTGTAATGGC+TGG | 0.563650 | 8.4:-14099650 | MS.gene59078:intron |
| TTTGTAATTCATGCATTGGA+GGG | 0.572592 | 8.4:+14099171 | None:intergenic |
| TGTGTAAACAGAGGGTGTAA+TGG | 0.582073 | 8.4:-14099654 | MS.gene59078:intron |
| GCCAAGCTGGTTCTCTCAAG+AGG | 0.593616 | 8.4:+14099803 | None:intergenic |
| AGTTACAGTTGGAATGGCAG+TGG | 0.597023 | 8.4:+14099598 | None:intergenic |
| TCTGGACCAGAGGTTGGCCA+TGG | 0.615026 | 8.4:-14099777 | MS.gene59078:CDS |
| ATTACCATGAGAACAGCGCT+TGG | 0.632062 | 8.4:+14099754 | None:intergenic |
| GCTTGGCTTATCTGGACCAG+AGG | 0.644191 | 8.4:-14099787 | MS.gene59078:CDS |
| AATTATGAGAGCTTGAGCAG+TGG | 0.660731 | 8.4:+14099371 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACTAAATACAATAACAA+AGG | + | chr8.4:14099328-14099347 | None:intergenic | 15.0% |
| !! | CAGAACTATATCAAAATTAA+TGG | + | chr8.4:14099478-14099497 | None:intergenic | 20.0% |
| !! | TCAATGTATAATGTAATCTT+TGG | - | chr8.4:14099299-14099318 | MS.gene59078:intron | 20.0% |
| !! | TTTCTTAGGATATTTAAAAG+TGG | + | chr8.4:14099525-14099544 | None:intergenic | 20.0% |
| !!! | CTTTTTGGTATATAATTATC+TGG | - | chr8.4:14099549-14099568 | MS.gene59078:intron | 20.0% |
| ! | TGAAGCAAAGAAAATTGAAT+AGG | + | chr8.4:14099450-14099469 | None:intergenic | 25.0% |
| !! | ATCCTAAGAAAGATACTTTT+TGG | - | chr8.4:14099534-14099553 | MS.gene59078:intron | 25.0% |
| !!! | TACCAAAAAGTATCTTTCTT+AGG | + | chr8.4:14099539-14099558 | None:intergenic | 25.0% |
| AAGAAAATTGAATAGGTGGA+AGG | + | chr8.4:14099443-14099462 | None:intergenic | 30.0% | |
| AGCAAAGAAAATTGAATAGG+TGG | + | chr8.4:14099447-14099466 | None:intergenic | 30.0% | |
| CCACTACAAACAAAATATGA+GGG | + | chr8.4:14099750-14099769 | None:intergenic | 30.0% | |
| ATTAGCAGCAACATTTCTCT+TGG | + | chr8.4:14099186-14099205 | None:intergenic | 35.0% | |
| CAAGCTCTCATAATTTCTAC+AGG | - | chr8.4:14099649-14099668 | MS.gene59078:intron | 35.0% | |
| GCCACTACAAACAAAATATG+AGG | + | chr8.4:14099751-14099770 | None:intergenic | 35.0% | |
| TGTATCAAGCAATGATTGTG+AGG | + | chr8.4:14099718-14099737 | None:intergenic | 35.0% | |
| ! | TTGATCAGTGTTGAGCAATA+AGG | + | chr8.4:14099688-14099707 | None:intergenic | 35.0% |
| !! | CATATTTTGTTTGTAGTGGC+TGG | - | chr8.4:14099751-14099770 | MS.gene59078:intron | 35.0% |
| !! | CCCTCATATTTTGTTTGTAG+TGG | - | chr8.4:14099747-14099766 | MS.gene59078:intron | 35.0% |
| AATTATGAGAGCTTGAGCAG+TGG | + | chr8.4:14099644-14099663 | None:intergenic | 40.0% | |
| AGGAGTAGTTACAGTTGGAA+TGG | + | chr8.4:14099423-14099442 | None:intergenic | 40.0% | |
| AGTAGCTGACAAGACTGTAT+TGG | - | chr8.4:14099789-14099808 | MS.gene59078:CDS | 40.0% | |
| ATATATGCTTGACATGCAGC+TGG | - | chr8.4:14099576-14099595 | MS.gene59078:intron | 40.0% | |
| ATTATGAGAGCTTGAGCAGT+GGG | + | chr8.4:14099643-14099662 | None:intergenic | 40.0% | |
| CCATTCCTTGTGTAAACAGA+GGG | - | chr8.4:14099350-14099369 | MS.gene59078:intron | 40.0% | |
| TAGTGTTAGGATGCTACCTT+GGG | - | chr8.4:14099600-14099619 | MS.gene59078:intron | 40.0% | |
| TCCATTCCTTGTGTAAACAG+AGG | - | chr8.4:14099349-14099368 | MS.gene59078:intron | 40.0% | |
| TTACACCCTCTGTTTACACA+AGG | + | chr8.4:14099358-14099377 | None:intergenic | 40.0% | |
| ! | ATCAGTGTTGAGCAATAAGG+AGG | + | chr8.4:14099685-14099704 | None:intergenic | 40.0% |
| ! | TGTGTAAACAGAGGGTGTAA+TGG | - | chr8.4:14099358-14099377 | MS.gene59078:intron | 40.0% |
| AGTTACAGTTGGAATGGCAG+TGG | + | chr8.4:14099417-14099436 | None:intergenic | 45.0% | |
| ATTACCATGAGAACAGCGCT+TGG | + | chr8.4:14099261-14099280 | None:intergenic | 45.0% | |
| CCCTCTGTTTACACAAGGAA+TGG | + | chr8.4:14099353-14099372 | None:intergenic | 45.0% | |
| CTAGTGTTAGGATGCTACCT+TGG | - | chr8.4:14099599-14099618 | MS.gene59078:intron | 45.0% | |
| GTGGAAGGAGTAGTTACAGT+TGG | + | chr8.4:14099428-14099447 | None:intergenic | 45.0% | |
| ! | TAAACAGAGGGTGTAATGGC+TGG | - | chr8.4:14099362-14099381 | MS.gene59078:intron | 45.0% |
| ACATGCAGCTGGCTAGTGTT+AGG | - | chr8.4:14099587-14099606 | MS.gene59078:intron | 50.0% | |
| GAGAACCAGCTTGGCTTATC+TGG | - | chr8.4:14099217-14099236 | MS.gene59078:CDS | 50.0% | |
| TCCTCTTGAGAGAACCAGCT+TGG | - | chr8.4:14099208-14099227 | MS.gene59078:CDS | 50.0% | |
| ! | TTCAGGTTTGCTCTTGCCCA+AGG | + | chr8.4:14099619-14099638 | None:intergenic | 50.0% |
| ATGAGAACAGCGCTTGGCCA+TGG | + | chr8.4:14099255-14099274 | None:intergenic | 55.0% | |
| CTGGTCCAGATAAGCCAAGC+TGG | + | chr8.4:14099225-14099244 | None:intergenic | 55.0% | |
| GAGCTTGAGCAGTGGGATTC+AGG | + | chr8.4:14099636-14099655 | None:intergenic | 55.0% | |
| GCTTGGCTTATCTGGACCAG+AGG | - | chr8.4:14099225-14099244 | MS.gene59078:CDS | 55.0% | |
| GGCTTATCTGGACCAGAGGT+TGG | - | chr8.4:14099229-14099248 | MS.gene59078:CDS | 55.0% | |
| GGGTGTAATGGCTGGAGCTA+AGG | - | chr8.4:14099370-14099389 | MS.gene59078:CDS | 55.0% | |
| TGCATTGGAGGGTCGGTTGA+TGG | + | chr8.4:14099833-14099852 | None:intergenic | 55.0% | |
| ! | ATGGCCAAGCGCTGTTCTCA+TGG | - | chr8.4:14099254-14099273 | MS.gene59078:intron | 55.0% |
| ! | GCCAAGCTGGTTCTCTCAAG+AGG | + | chr8.4:14099212-14099231 | None:intergenic | 55.0% |
| ! | TCTGGACCAGAGGTTGGCCA+TGG | - | chr8.4:14099235-14099254 | MS.gene59078:CDS | 60.0% |
| GCTTGGCCATGGCCAACCTC+TGG | + | chr8.4:14099244-14099263 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 14099179 | 14099855 | 14099179 | ID=MS.gene59078 |
| chr8.4 | mRNA | 14099179 | 14099855 | 14099179 | ID=MS.gene59078.t1;Parent=MS.gene59078 |
| chr8.4 | exon | 14099759 | 14099855 | 14099759 | ID=MS.gene59078.t1.exon1;Parent=MS.gene59078.t1 |
| chr8.4 | CDS | 14099759 | 14099855 | 14099759 | ID=cds.MS.gene59078.t1;Parent=MS.gene59078.t1 |
| chr8.4 | exon | 14099604 | 14099665 | 14099604 | ID=MS.gene59078.t1.exon2;Parent=MS.gene59078.t1 |
| chr8.4 | CDS | 14099604 | 14099665 | 14099604 | ID=cds.MS.gene59078.t1;Parent=MS.gene59078.t1 |
| chr8.4 | exon | 14099364 | 14099439 | 14099364 | ID=MS.gene59078.t1.exon3;Parent=MS.gene59078.t1 |
| chr8.4 | CDS | 14099364 | 14099439 | 14099364 | ID=cds.MS.gene59078.t1;Parent=MS.gene59078.t1 |
| chr8.4 | exon | 14099179 | 14099267 | 14099179 | ID=MS.gene59078.t1.exon4;Parent=MS.gene59078.t1 |
| chr8.4 | CDS | 14099179 | 14099267 | 14099179 | ID=cds.MS.gene59078.t1;Parent=MS.gene59078.t1 |
| Gene Sequence |
| Protein sequence |
