Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055157.t1 | XP_003623166.1 | 95.7 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 9.10E-58 | 232.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055157.t1 | G7L5L7 | 95.7 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 6.5e-58 | 232.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050426 | MS.gene055157 | 0.813302 | 2.74E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene055157 | 0.809203 | 2.12E-50 | -1.69E-46 |
| MS.gene052223 | MS.gene055157 | 0.801464 | 8.89E-49 | -1.69E-46 |
| MS.gene055157 | MS.gene055158 | 0.817108 | 3.91E-52 | -1.69E-46 |
| MS.gene055157 | MS.gene055159 | 0.863254 | 2.84E-64 | -1.69E-46 |
| MS.gene055157 | MS.gene055601 | 0.800181 | 1.63E-48 | -1.69E-46 |
| MS.gene055157 | MS.gene056899 | 0.800746 | 1.25E-48 | -1.69E-46 |
| MS.gene055157 | MS.gene062491 | 0.825225 | 5.26E-54 | -1.69E-46 |
| MS.gene055157 | MS.gene30518 | 0.815443 | 9.22E-52 | -1.69E-46 |
| MS.gene055157 | MS.gene30519 | 0.811412 | 7.09E-51 | -1.69E-46 |
| MS.gene055157 | MS.gene30520 | 0.818792 | 1.63E-52 | -1.69E-46 |
| MS.gene055157 | MS.gene33522 | 0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene055157 | MS.gene44958 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene055157 | MS.gene54611 | 0.801429 | 9.04E-49 | -1.69E-46 |
| MS.gene055157 | MS.gene57737 | 0.825487 | 4.56E-54 | -1.69E-46 |
| MS.gene055157 | MS.gene58740 | 0.820191 | 7.81E-53 | -1.69E-46 |
| MS.gene055157 | MS.gene59078 | 0.846775 | 1.76E-59 | -1.69E-46 |
| MS.gene055157 | MS.gene62454 | 0.85548 | 6.13E-62 | -1.69E-46 |
| MS.gene055157 | MS.gene62885 | 0.82368 | 1.22E-53 | -1.69E-46 |
| MS.gene055157 | MS.gene62886 | 0.823718 | 1.19E-53 | -1.69E-46 |
| MS.gene055157 | MS.gene70064 | 0.801179 | 1.02E-48 | -1.69E-46 |
| MS.gene055157 | MS.gene71924 | 0.835005 | 2.16E-56 | -1.69E-46 |
| MS.gene055157 | MS.gene71927 | 0.823434 | 1.39E-53 | -1.69E-46 |
| MS.gene055157 | MS.gene72924 | 0.821399 | 4.12E-53 | -1.69E-46 |
| MS.gene055157 | MS.gene74032 | 0.832319 | 1.01E-55 | -1.69E-46 |
| MS.gene055157 | MS.gene79232 | 0.800095 | 1.69E-48 | -1.69E-46 |
| MS.gene055157 | MS.gene83008 | 0.820872 | 5.45E-53 | -1.69E-46 |
| MS.gene055157 | MS.gene83011 | 0.835532 | 1.59E-56 | -1.69E-46 |
| MS.gene055157 | MS.gene85276 | 0.809882 | 1.52E-50 | -1.69E-46 |
| MS.gene055157 | MS.gene89264 | 0.818876 | 1.56E-52 | -1.69E-46 |
| MS.gene055157 | MS.gene89267 | 0.830915 | 2.24E-55 | -1.69E-46 |
| MS.gene055157 | MS.gene90104 | 0.818266 | 2.14E-52 | -1.69E-46 |
| MS.gene055157 | MS.gene92106 | -0.80164 | 8.19E-49 | -1.69E-46 |
| MS.gene055157 | MS.gene94255 | 0.804764 | 1.85E-49 | -1.69E-46 |
| MS.gene055157 | MS.gene94256 | 0.82356 | 1.30E-53 | -1.69E-46 |
| MS.gene055157 | MS.gene94257 | 0.856937 | 2.30E-62 | -1.69E-46 |
| MS.gene055157 | MS.gene99544 | 0.80077 | 1.23E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055157.t1 | MTR_7g065210 | 95.726 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 7.14e-81 | 233 |
| MS.gene055157.t1 | MTR_7g065160 | 85.470 | 117 | 17 | 0 | 1 | 117 | 1 | 117 | 1.23e-73 | 214 |
| MS.gene055157.t1 | MTR_8g070040 | 53.684 | 95 | 42 | 2 | 24 | 117 | 27 | 120 | 1.31e-26 | 95.9 |
| MS.gene055157.t1 | MTR_8g070015 | 50.000 | 88 | 42 | 2 | 24 | 110 | 27 | 113 | 5.86e-21 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055157.t1 | AT3G53980 | 69.474 | 95 | 28 | 1 | 23 | 117 | 21 | 114 | 1.30e-45 | 143 |
| MS.gene055157.t1 | AT3G53980 | 69.474 | 95 | 28 | 1 | 23 | 117 | 21 | 114 | 1.30e-45 | 143 |
| MS.gene055157.t1 | AT5G05960 | 58.120 | 117 | 44 | 2 | 5 | 117 | 1 | 116 | 7.16e-43 | 137 |
| MS.gene055157.t1 | AT2G37870 | 47.706 | 109 | 53 | 3 | 11 | 117 | 9 | 115 | 9.80e-27 | 95.9 |
Find 23 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCATTATC+TGG | 0.246677 | 7.4:-39443432 | None:intergenic |
| TAGCAAAGTTGCAGCGTTTA+GGG | 0.296288 | 7.4:-39443617 | None:intergenic |
| GTCCTTGTTGCAGGGATTCA+TGG | 0.338655 | 7.4:+39443372 | MS.gene055157:CDS |
| TTAGCAAAGTTGCAGCGTTT+AGG | 0.339318 | 7.4:-39443618 | None:intergenic |
| TTTCTTAGTCTGAGCACAAC+AGG | 0.455007 | 7.4:-39443505 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.4:+39443567 | MS.gene055157:CDS |
| GGATAGTAATAGCAATTTGA+GGG | 0.469235 | 7.4:-39443596 | None:intergenic |
| GGGATAGTAATAGCAATTTG+AGG | 0.475358 | 7.4:-39443597 | None:intergenic |
| AACTTTGCTAATCGTCCCAT+TGG | 0.509591 | 7.4:+39443630 | MS.gene055157:CDS |
| CGTCCGCACTTATAACCAAT+GGG | 0.524534 | 7.4:-39443645 | None:intergenic |
| ACGTCCGCACTTATAACCAA+TGG | 0.525340 | 7.4:-39443646 | None:intergenic |
| ATGAAGATGATGTGCCTAGT+TGG | 0.533861 | 7.4:+39443342 | MS.gene055157:CDS |
| GTTGAAAGTGCTGGTGAATG+TGG | 0.534504 | 7.4:+39443396 | MS.gene055157:CDS |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7.4:-39443459 | None:intergenic |
| AAAGTGCTGGTGAATGTGGA+AGG | 0.549386 | 7.4:+39443400 | MS.gene055157:CDS |
| CAAGGACTACAAAACCAACT+AGG | 0.588924 | 7.4:-39443356 | None:intergenic |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.4:-39443540 | None:intergenic |
| CTCCATGAATCCCTGCAACA+AGG | 0.594877 | 7.4:-39443374 | None:intergenic |
| TGTGCTCAGACTAAGAAACT+TGG | 0.609770 | 7.4:+39443510 | MS.gene055157:CDS |
| ATTCATGGAGTTGAAAGTGC+TGG | 0.616969 | 7.4:+39443387 | MS.gene055157:CDS |
| AGTGCTGGTGAATGTGGAAG+GGG | 0.630405 | 7.4:+39443402 | MS.gene055157:CDS |
| AAGTGCTGGTGAATGTGGAA+GGG | 0.641454 | 7.4:+39443401 | MS.gene055157:CDS |
| CGTCCCATTGGTTATAAGTG+CGG | 0.667176 | 7.4:+39443642 | MS.gene055157:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAGAAATATATTAATAT+AGG | + | chr7.4:39443855-39443874 | MS.gene055157:intron | 10.0% |
| !! | TATATTAATATAGGAAAAAA+AGG | + | chr7.4:39443864-39443883 | MS.gene055157:intron | 10.0% |
| !! | AAAATAAAAAAAATAACACG+TGG | + | chr7.4:39444032-39444051 | MS.gene055157:intron | 15.0% |
| !! | AAGAATAAATATAAACGAAA+TGG | - | chr7.4:39443725-39443744 | None:intergenic | 15.0% |
| !!! | ATTTTGTAGTTTTTTTTCTT+AGG | - | chr7.4:39443941-39443960 | None:intergenic | 15.0% |
| !!! | TTTAGTAGTTTTTTTTTCTT+AGG | + | chr7.4:39444145-39444164 | MS.gene055157:intron | 15.0% |
| !!! | TTTTATTTTTAAATTCATGC+AGG | - | chr7.4:39444021-39444040 | None:intergenic | 15.0% |
| !!! | TTTTGTAGTTTTTTTTCTTA+GGG | - | chr7.4:39443940-39443959 | None:intergenic | 15.0% |
| !! | AACAACAAAAAAATGTGAAA+CGG | + | chr7.4:39444240-39444259 | MS.gene055157:intron | 20.0% |
| !!! | CTATTTGTTAATTTTCTTGA+TGG | + | chr7.4:39444301-39444320 | MS.gene055157:intron | 20.0% |
| !!! | TTTGTAGTTTTTTTTCTTAG+GGG | - | chr7.4:39443939-39443958 | None:intergenic | 20.0% |
| !! | AATCGCAACTTTGAAAATTT+AGG | + | chr7.4:39444175-39444194 | MS.gene055157:intron | 25.0% |
| !! | AATTTTCAAAGTTGCGATTT+TGG | - | chr7.4:39444174-39444193 | None:intergenic | 25.0% |
| !! | ATCGCAACTTTGAAAATTTA+GGG | + | chr7.4:39444176-39444195 | MS.gene055157:intron | 25.0% |
| !!! | AGTAGTTTTTTTTTCTTAGG+AGG | + | chr7.4:39444148-39444167 | MS.gene055157:intron | 25.0% |
| !!! | TTGTAGTTTTTTTTCTTAGG+GGG | - | chr7.4:39443938-39443957 | None:intergenic | 25.0% |
| AGCTTAAATGCTTCATTATC+TGG | - | chr7.4:39443435-39443454 | None:intergenic | 30.0% | |
| GACAACTTAAAAGAAACTGA+GGG | - | chr7.4:39443687-39443706 | None:intergenic | 30.0% | |
| GGATAGTAATAGCAATTTGA+GGG | - | chr7.4:39443599-39443618 | None:intergenic | 30.0% | |
| AAAACTGCACAAAGACAACT+TGG | - | chr7.4:39443543-39443562 | None:intergenic | 35.0% | |
| CGACAACTTAAAAGAAACTG+AGG | - | chr7.4:39443688-39443707 | None:intergenic | 35.0% | |
| CTAAAATCACAACTTCGTGA+AGG | - | chr7.4:39443915-39443934 | None:intergenic | 35.0% | |
| GGGATAGTAATAGCAATTTG+AGG | - | chr7.4:39443600-39443619 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7.4:39443567-39443586 | MS.gene055157:CDS | 35.0% | |
| TGCTACAGTTGCAAAACTTA+GGG | - | chr7.4:39443968-39443987 | None:intergenic | 35.0% | |
| ! | TTTTGCAACTGTAGCAGTTT+TGG | + | chr7.4:39443972-39443991 | MS.gene055157:intron | 35.0% |
| AACTTTGCTAATCGTCCCAT+TGG | + | chr7.4:39443630-39443649 | MS.gene055157:CDS | 40.0% | |
| ATCACAACTTCGTGAAGGTT+AGG | - | chr7.4:39443910-39443929 | None:intergenic | 40.0% | |
| ATGAAGATGATGTGCCTAGT+TGG | + | chr7.4:39443342-39443361 | MS.gene055157:CDS | 40.0% | |
| CAAGGACTACAAAACCAACT+AGG | - | chr7.4:39443359-39443378 | None:intergenic | 40.0% | |
| CTACAGTTGCAAAACTTAGG+GGG | - | chr7.4:39443966-39443985 | None:intergenic | 40.0% | |
| CTGCTACAGTTGCAAAACTT+AGG | - | chr7.4:39443969-39443988 | None:intergenic | 40.0% | |
| GCTACAGTTGCAAAACTTAG+GGG | - | chr7.4:39443967-39443986 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | - | chr7.4:39443620-39443639 | None:intergenic | 40.0% | |
| TCACAACTTCGTGAAGGTTA+GGG | - | chr7.4:39443909-39443928 | None:intergenic | 40.0% | |
| TCATGCAGGTGCAAAAAACT+TGG | - | chr7.4:39444007-39444026 | None:intergenic | 40.0% | |
| TGTGCTCAGACTAAGAAACT+TGG | + | chr7.4:39443510-39443529 | MS.gene055157:CDS | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | - | chr7.4:39443621-39443640 | None:intergenic | 40.0% | |
| TTTCTTAGTCTGAGCACAAC+AGG | - | chr7.4:39443508-39443527 | None:intergenic | 40.0% | |
| ! | ATTCATGGAGTTGAAAGTGC+TGG | + | chr7.4:39443387-39443406 | MS.gene055157:CDS | 40.0% |
| ! | GTTTTGTAGTCCTTGTTGCA+GGG | + | chr7.4:39443364-39443383 | MS.gene055157:CDS | 40.0% |
| AACACGTGGCACCTCATATA+GGG | + | chr7.4:39444046-39444065 | MS.gene055157:intron | 45.0% | |
| ACAACTTCGTGAAGGTTAGG+GGG | - | chr7.4:39443907-39443926 | None:intergenic | 45.0% | |
| ACGTCCGCACTTATAACCAA+TGG | - | chr7.4:39443649-39443668 | None:intergenic | 45.0% | |
| AGCGTAGACCGAGTCAAAAT+TGG | + | chr7.4:39444078-39444097 | MS.gene055157:intron | 45.0% | |
| AGGTGCAAAAAACTTGGCCT+TGG | - | chr7.4:39444001-39444020 | None:intergenic | 45.0% | |
| CACAACTTCGTGAAGGTTAG+GGG | - | chr7.4:39443908-39443927 | None:intergenic | 45.0% | |
| CGTCCGCACTTATAACCAAT+GGG | - | chr7.4:39443648-39443667 | None:intergenic | 45.0% | |
| TAACACGTGGCACCTCATAT+AGG | + | chr7.4:39444045-39444064 | MS.gene055157:intron | 45.0% | |
| ! | CGTCCCATTGGTTATAAGTG+CGG | + | chr7.4:39443642-39443661 | MS.gene055157:CDS | 45.0% |
| ! | GGTGCAAAAAACTTGGCCTT+GGG | - | chr7.4:39444000-39444019 | None:intergenic | 45.0% |
| !! | AAAGTGCTGGTGAATGTGGA+AGG | + | chr7.4:39443400-39443419 | MS.gene055157:CDS | 45.0% |
| !! | AAGTGCTGGTGAATGTGGAA+GGG | + | chr7.4:39443401-39443420 | MS.gene055157:CDS | 45.0% |
| !! | ACCGAGTCAAAATTGGCCTT+GGG | + | chr7.4:39444085-39444104 | MS.gene055157:intron | 45.0% |
| !! | GGTTTTGTAGTCCTTGTTGC+AGG | + | chr7.4:39443363-39443382 | MS.gene055157:CDS | 45.0% |
| !! | GTGCAAAAAACTTGGCCTTG+GGG | - | chr7.4:39443999-39444018 | None:intergenic | 45.0% |
| !! | GTTGAAAGTGCTGGTGAATG+TGG | + | chr7.4:39443396-39443415 | MS.gene055157:CDS | 45.0% |
| !! | TGCAAAAAACTTGGCCTTGG+GGG | - | chr7.4:39443998-39444017 | None:intergenic | 45.0% |
| CCACCGGTGCAAAACTTAGA+GGG | + | chr7.4:39444119-39444138 | MS.gene055157:intron | 50.0% | |
| CTCCATGAATCCCTGCAACA+AGG | - | chr7.4:39443377-39443396 | None:intergenic | 50.0% | |
| CTGATGTGGCACCCTATATG+AGG | - | chr7.4:39444060-39444079 | None:intergenic | 50.0% | |
| GTCCTTGTTGCAGGGATTCA+TGG | + | chr7.4:39443372-39443391 | MS.gene055157:CDS | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | - | chr7.4:39443462-39443481 | None:intergenic | 50.0% | |
| ! | GACCGAGTCAAAATTGGCCT+TGG | + | chr7.4:39444084-39444103 | MS.gene055157:intron | 50.0% |
| !! | AGTGCTGGTGAATGTGGAAG+GGG | + | chr7.4:39443402-39443421 | MS.gene055157:CDS | 50.0% |
| !! | CCGAGTCAAAATTGGCCTTG+GGG | + | chr7.4:39444086-39444105 | MS.gene055157:intron | 50.0% |
| !! | TTTTGCACCGGTGGCAGTTT+TGG | - | chr7.4:39444113-39444132 | None:intergenic | 50.0% |
| !!! | CCCCAAGGCCAATTTTGACT+CGG | - | chr7.4:39444089-39444108 | None:intergenic | 50.0% |
| CCGGTGCAAAACTTAGAGGG+CGG | + | chr7.4:39444122-39444141 | MS.gene055157:intron | 55.0% | |
| GCCACCGGTGCAAAACTTAG+AGG | + | chr7.4:39444118-39444137 | MS.gene055157:intron | 55.0% | |
| TTGGGGACCAAAACTGCCAC+CGG | + | chr7.4:39444103-39444122 | MS.gene055157:intron | 55.0% | |
| ! | CCCTCTAAGTTTTGCACCGG+TGG | - | chr7.4:39444122-39444141 | None:intergenic | 55.0% |
| ! | CCGCCCTCTAAGTTTTGCAC+CGG | - | chr7.4:39444125-39444144 | None:intergenic | 55.0% |
| !! | TGACTCGGTCTACGCTGATG+TGG | - | chr7.4:39444074-39444093 | None:intergenic | 55.0% |
| !! | TGTAGCAGTTTTGGCCCCCA+AGG | + | chr7.4:39443981-39444000 | MS.gene055157:intron | 55.0% |
| !! | GGTGGCAGTTTTGGTCCCCA+AGG | - | chr7.4:39444104-39444123 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 39443330 | 39444381 | 39443330 | ID=MS.gene055157 |
| chr7.4 | mRNA | 39443330 | 39444381 | 39443330 | ID=MS.gene055157.t1;Parent=MS.gene055157 |
| chr7.4 | exon | 39443330 | 39443666 | 39443330 | ID=MS.gene055157.t1.exon1;Parent=MS.gene055157.t1 |
| chr7.4 | CDS | 39443330 | 39443666 | 39443330 | ID=cds.MS.gene055157.t1;Parent=MS.gene055157.t1 |
| chr7.4 | exon | 39444365 | 39444381 | 39444365 | ID=MS.gene055157.t1.exon2;Parent=MS.gene055157.t1 |
| chr7.4 | CDS | 39444365 | 39444381 | 39444365 | ID=cds.MS.gene055157.t1;Parent=MS.gene055157.t1 |
| Gene Sequence |
| Protein sequence |
