AlfalfaGEDB | MS.gene59408

M. sativa cultivar XinJiangDaYe / MS.gene59408

KEGG: -
GO: GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0030001 GO:0043167 GO:0043169 GO:0046872 GO:0051179 GO:0051234
PFAM: PF00403.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59408.t1	XP_003612070.1	79	157	12	1	1	136	1	157	3.20E-46	194.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59408.t1	Q0WV37	41.0	61	36	0	6	66	13	73	5.0e-08	58.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59408.t1	G7KF08	79.0	157	12	1	1	136	1	157	2.3e-46	194.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050082	MS.gene59408	0.815796	7.69E-52	-1.69E-46
MS.gene058111	MS.gene59408	0.814271	1.68E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59408.t1	MTR_5g020960	83.439	157	5	1	1	136	1	157	6.11e-87	250
MS.gene59408.t1	MTR_2g022250	41.429	70	41	0	2	71	9	78	3.04e-12	62.8
MS.gene59408.t1	MTR_4g117140	39.437	71	43	0	2	72	9	79	8.18e-12	61.6
MS.gene59408.t1	MTR_4g117220	39.437	71	43	0	2	72	9	79	8.46e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59408.t1	AT3G06130	37.975	79	47	1	2	80	9	85	1.82e-11	60.5
MS.gene59408.t1	AT5G19090	39.189	74	45	0	2	75	9	82	2.33e-11	60.5
MS.gene59408.t1	AT5G19090	39.189	74	45	0	2	75	9	82	2.33e-11	60.5
MS.gene59408.t1	AT5G19090	39.189	74	45	0	2	75	9	82	2.40e-11	60.5
MS.gene59408.t1	AT5G19090	39.189	74	45	0	2	75	9	82	2.40e-11	60.5
MS.gene59408.t1	AT5G19090	39.189	74	45	0	2	75	9	82	2.40e-11	60.5
MS.gene59408.t1	AT3G06130	37.975	79	47	1	2	80	9	85	2.80e-11	60.1
MS.gene59408.t1	AT3G05220	38.462	65	40	0	2	66	9	73	4.49e-11	59.7

Find 38 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGGAACATTGACAATATT+AGG	0.225728	5.4:+12817621	MS.gene59408:CDS
TATAGGGCACATAAGATATT+TGG	0.287159	5.4:-12817854	None:intergenic
CATAATAATGCAAGATGTAT+TGG	0.312448	5.4:-12817952	None:intergenic
ATCCGAACTATTATGTCATA+TGG	0.314119	5.4:-12817654	None:intergenic
TAAGCCCAGCCCAGTGAGTT+TGG	0.325149	5.4:+12817830	MS.gene59408:CDS
TGGTCCAAACTCACTGGGCT+GGG	0.376798	5.4:-12817834	None:intergenic
TGGTACTGTGGGTAGTAATA+AGG	0.380119	5.4:-12817885	None:intergenic
AGGGAACATTGACAATATTA+GGG	0.387340	5.4:+12817622	MS.gene59408:CDS
GTAATAAGGCATCTGCGTAT+AGG	0.404741	5.4:-12817871	None:intergenic
AAAACTGTCTCTTCTCTTGA+AGG	0.408686	5.4:+12817405	MS.gene59408:CDS
TAATAAGGCATCTGCGTATA+GGG	0.410219	5.4:-12817870	None:intergenic
ACTGGCTGGCCCTGGTACTG+TGG	0.417755	5.4:-12817897	None:intergenic
ATAATAATGCAAGATGTATT+GGG	0.421751	5.4:-12817951	None:intergenic
TTCATGTGCACCACAGCAAC+TGG	0.422470	5.4:-12817915	None:intergenic
AAAGAGTAAGCCTGGTGATA+AGG	0.441480	5.4:+12817806	MS.gene59408:CDS
GGTCAAGAAAACTGTCTTGA+AGG	0.445221	5.4:+12817344	MS.gene59408:CDS
TTGGTCCAAACTCACTGGGC+TGG	0.450959	5.4:-12817835	None:intergenic
ATGACATAATAGTTCGGATA+AGG	0.460277	5.4:+12817658	MS.gene59408:CDS
GATATTTGGTCCAAACTCAC+TGG	0.465365	5.4:-12817840	None:intergenic
TTATTACTACCCACAGTACC+AGG	0.477048	5.4:+12817887	MS.gene59408:CDS
CTGGGCTTATCCTTATCACC+AGG	0.486005	5.4:-12817816	None:intergenic
CCAGGGCCAGCCAGTTGCTG+TGG	0.497168	5.4:+12817905	MS.gene59408:CDS
CTGGCTGGCCCTGGTACTGT+GGG	0.497483	5.4:-12817896	None:intergenic
CCCGAAGAAAAGAGTAAGCC+TGG	0.497932	5.4:+12817798	MS.gene59408:CDS
CCACAGCAACTGGCTGGCCC+TGG	0.503535	5.4:-12817905	None:intergenic
TTGCTGTGGTGCACATGAAT+AGG	0.509643	5.4:+12817919	MS.gene59408:CDS
ACCCATATGACATAATAGTT+CGG	0.515071	5.4:+12817652	MS.gene59408:CDS
TCCGAACTATTATGTCATAT+GGG	0.521231	5.4:-12817653	None:intergenic
TGTGCACCACAGCAACTGGC+TGG	0.525693	5.4:-12817911	None:intergenic
ATAGTTCGGATAAGGAAAGC+AGG	0.528580	5.4:+12817666	MS.gene59408:CDS
AATTGAAGCTGATGAAGTGA+AGG	0.538003	5.4:+12817602	MS.gene59408:CDS
CTGTGGTGCACATGAATAGG+TGG	0.563070	5.4:+12817922	MS.gene59408:CDS
ATTGAAGCTGATGAAGTGAA+GGG	0.616569	5.4:+12817603	MS.gene59408:CDS
ATATTTGGTCCAAACTCACT+GGG	0.624772	5.4:-12817839	None:intergenic
TAGTTCGGATAAGGAAAGCA+GGG	0.637627	5.4:+12817667	MS.gene59408:CDS
TGTGGTGCACATGAATAGGT+GGG	0.692350	5.4:+12817923	MS.gene59408:CDS
TATTACTACCCACAGTACCA+GGG	0.693803	5.4:+12817888	MS.gene59408:CDS
GGGAACATTGACAATATTAG+GGG	0.701854	5.4:+12817623	MS.gene59408:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATTGAATTAACATTTAAAA+TGG	+	chr5.4:12817504-12817523	MS.gene59408:intron	15.0%
!!	ATAATAATGCAAGATGTATT+GGG	-	chr5.4:12817954-12817973	None:intergenic	20.0%
!!!	AATTTTTTTCATTGAATTGC+AGG	+	chr5.4:12817570-12817589	MS.gene59408:intron	20.0%
!!!	ATTTTTTTCATTGAATTGCA+GGG	+	chr5.4:12817571-12817590	MS.gene59408:intron	20.0%
!	CATAATAATGCAAGATGTAT+TGG	-	chr5.4:12817955-12817974	None:intergenic	25.0%
!!	ATGAGTTTCTTTTTGCATTT+GGG	-	chr5.4:12817384-12817403	None:intergenic	25.0%
	AAGGGAACATTGACAATATT+AGG	+	chr5.4:12817621-12817640	MS.gene59408:CDS	30.0%
	ACCCATATGACATAATAGTT+CGG	+	chr5.4:12817652-12817671	MS.gene59408:CDS	30.0%
	AGGAGATTAAGTACTAATGT+AGG	-	chr5.4:12817458-12817477	None:intergenic	30.0%
	AGGGAACATTGACAATATTA+GGG	+	chr5.4:12817622-12817641	MS.gene59408:CDS	30.0%
	ATCCGAACTATTATGTCATA+TGG	-	chr5.4:12817657-12817676	None:intergenic	30.0%
	ATGACATAATAGTTCGGATA+AGG	+	chr5.4:12817658-12817677	MS.gene59408:CDS	30.0%
	CATAACAAAAATAGTGAGAG+AGG	-	chr5.4:12817484-12817503	None:intergenic	30.0%
	TATAGGGCACATAAGATATT+TGG	-	chr5.4:12817857-12817876	None:intergenic	30.0%
	TCCGAACTATTATGTCATAT+GGG	-	chr5.4:12817656-12817675	None:intergenic	30.0%
	TGTGTATAATCTAAATGCTG+TGG	-	chr5.4:12817434-12817453	None:intergenic	30.0%
!	GATGAGTTTCTTTTTGCATT+TGG	-	chr5.4:12817385-12817404	None:intergenic	30.0%
!!!	CTCTCTCACTATTTTTGTTA+TGG	+	chr5.4:12817482-12817501	MS.gene59408:intron	30.0%
	AAAAATAGTGAGAGAGGAGA+AGG	-	chr5.4:12817478-12817497	None:intergenic	35.0%
	AAAACTGTCTCTTCTCTTGA+AGG	+	chr5.4:12817405-12817424	MS.gene59408:CDS	35.0%
	AATTGAAGCTGATGAAGTGA+AGG	+	chr5.4:12817602-12817621	MS.gene59408:CDS	35.0%
	ATATTTGGTCCAAACTCACT+GGG	-	chr5.4:12817842-12817861	None:intergenic	35.0%
	ATGCTGAAGTTGTGAGTATA+GGG	+	chr5.4:12817694-12817713	MS.gene59408:intron	35.0%
	ATTGAAGCTGATGAAGTGAA+GGG	+	chr5.4:12817603-12817622	MS.gene59408:CDS	35.0%
	GGGAACATTGACAATATTAG+GGG	+	chr5.4:12817623-12817642	MS.gene59408:CDS	35.0%
!	TAATAAGGCATCTGCGTATA+GGG	-	chr5.4:12817873-12817892	None:intergenic	35.0%
!!!	TTTTTTGCAGCATCCTGTTT+CGG	-	chr5.4:12817759-12817778	None:intergenic	35.0%
	AAAGAGTAAGCCTGGTGATA+AGG	+	chr5.4:12817806-12817825	MS.gene59408:CDS	40.0%
	ATAGTTCGGATAAGGAAAGC+AGG	+	chr5.4:12817666-12817685	MS.gene59408:CDS	40.0%
	CATGCTGAAGTTGTGAGTAT+AGG	+	chr5.4:12817693-12817712	MS.gene59408:intron	40.0%
	GATATTTGGTCCAAACTCAC+TGG	-	chr5.4:12817843-12817862	None:intergenic	40.0%
	GGTCAAGAAAACTGTCTTGA+AGG	+	chr5.4:12817344-12817363	MS.gene59408:CDS	40.0%
	TAGTTCGGATAAGGAAAGCA+GGG	+	chr5.4:12817667-12817686	MS.gene59408:CDS	40.0%
	TATTACTACCCACAGTACCA+GGG	+	chr5.4:12817888-12817907	MS.gene59408:CDS	40.0%
	TGGTACTGTGGGTAGTAATA+AGG	-	chr5.4:12817888-12817907	None:intergenic	40.0%
	TTATTACTACCCACAGTACC+AGG	+	chr5.4:12817887-12817906	MS.gene59408:CDS	40.0%
!	ACCAGGCTTACTCTTTTCTT+CGG	-	chr5.4:12817802-12817821	None:intergenic	40.0%
!	GTAATAAGGCATCTGCGTAT+AGG	-	chr5.4:12817874-12817893	None:intergenic	40.0%
!!!	TCGGGCTTTTTATTTTCTGC+AGG	-	chr5.4:12817783-12817802	None:intergenic	40.0%
	TGTGGTGCACATGAATAGGT+GGG	+	chr5.4:12817923-12817942	MS.gene59408:CDS	45.0%
	TTGCTGTGGTGCACATGAAT+AGG	+	chr5.4:12817919-12817938	MS.gene59408:CDS	45.0%
!	CCAGGCTTACTCTTTTCTTC+GGG	-	chr5.4:12817801-12817820	None:intergenic	45.0%
	CCCGAAGAAAAGAGTAAGCC+TGG	+	chr5.4:12817798-12817817	MS.gene59408:CDS	50.0%
	CTGGGCTTATCCTTATCACC+AGG	-	chr5.4:12817819-12817838	None:intergenic	50.0%
	CTGTGGTGCACATGAATAGG+TGG	+	chr5.4:12817922-12817941	MS.gene59408:CDS	50.0%
	TTCATGTGCACCACAGCAAC+TGG	-	chr5.4:12817918-12817937	None:intergenic	50.0%
	TTTGCAGCATCCTGTTTCGG+AGG	-	chr5.4:12817756-12817775	None:intergenic	50.0%
	TAAGCCCAGCCCAGTGAGTT+TGG	+	chr5.4:12817830-12817849	MS.gene59408:CDS	55.0%
	TGGTCCAAACTCACTGGGCT+GGG	-	chr5.4:12817837-12817856	None:intergenic	55.0%
	TTGGTCCAAACTCACTGGGC+TGG	-	chr5.4:12817838-12817857	None:intergenic	55.0%
	TGTGCACCACAGCAACTGGC+TGG	-	chr5.4:12817914-12817933	None:intergenic	60.0%
	GTGAGTATAGGGCCTCCCCC+AGG	+	chr5.4:12817705-12817724	MS.gene59408:intron	65.0%
!	ACTGGCTGGCCCTGGTACTG+TGG	-	chr5.4:12817900-12817919	None:intergenic	65.0%
!	CTGGCTGGCCCTGGTACTGT+GGG	-	chr5.4:12817899-12817918	None:intergenic	65.0%
!	GCATCCTGTTTCGGAGGCGC+CGG	-	chr5.4:12817750-12817769	None:intergenic	65.0%
!!	CATCCTGTTTCGGAGGCGCC+GGG	-	chr5.4:12817749-12817768	None:intergenic	65.0%
	CCAGGGCCAGCCAGTTGCTG+TGG	+	chr5.4:12817905-12817924	MS.gene59408:CDS	70.0%
!	CCACAGCAACTGGCTGGCCC+TGG	-	chr5.4:12817908-12817927	None:intergenic	70.0%
	AGGTGGAGGGGCAGGGCCTG+GGG	-	chr5.4:12817724-12817743	None:intergenic	75.0%
	CAGGTGGAGGGGCAGGGCCT+GGG	-	chr5.4:12817725-12817744	None:intergenic	75.0%
	TGGCCCGGCGCCTCCGAAAC+AGG	+	chr5.4:12817743-12817762	MS.gene59408:intron	75.0%
!!	TGTTTCGGAGGCGCCGGGCC+AGG	-	chr5.4:12817744-12817763	None:intergenic	75.0%
!	CCAGGCCCTGCCCCTCCACC+TGG	+	chr5.4:12817723-12817742	MS.gene59408:intron	80.0%
!	CCAGGTGGAGGGGCAGGGCC+TGG	-	chr5.4:12817726-12817745	None:intergenic	80.0%
!	CCCTGCCCCTCCACCTGGCC+CGG	+	chr5.4:12817728-12817747	MS.gene59408:intron	80.0%
!	CCGGGCCAGGTGGAGGGGCA+GGG	-	chr5.4:12817731-12817750	None:intergenic	80.0%
!	GGTGGAGGGGCAGGGCCTGG+GGG	-	chr5.4:12817723-12817742	None:intergenic	80.0%
!!!	AGGCGCCGGGCCAGGTGGAG+GGG	-	chr5.4:12817736-12817755	None:intergenic	80.0%
!!!	GAGGCGCCGGGCCAGGTGGA+GGG	-	chr5.4:12817737-12817756	None:intergenic	80.0%
!!!	TTCGGAGGCGCCGGGCCAGG+TGG	-	chr5.4:12817741-12817760	None:intergenic	80.0%
!	GCCGGGCCAGGTGGAGGGGC+AGG	-	chr5.4:12817732-12817751	None:intergenic	85.0%
!	GGAGGGGCAGGGCCTGGGGG+AGG	-	chr5.4:12817720-12817739	None:intergenic	85.0%
!!!	GGAGGCGCCGGGCCAGGTGG+AGG	-	chr5.4:12817738-12817757	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	12817342	12817977	12817342	ID=MS.gene59408
chr5.4	mRNA	12817342	12817977	12817342	ID=MS.gene59408.t1;Parent=MS.gene59408
chr5.4	exon	12817342	12817426	12817342	ID=MS.gene59408.t1.exon1;Parent=MS.gene59408.t1
chr5.4	CDS	12817342	12817426	12817342	ID=cds.MS.gene59408.t1;Parent=MS.gene59408.t1
chr5.4	exon	12817592	12817704	12817592	ID=MS.gene59408.t1.exon2;Parent=MS.gene59408.t1
chr5.4	CDS	12817592	12817704	12817592	ID=cds.MS.gene59408.t1;Parent=MS.gene59408.t1
chr5.4	exon	12817765	12817977	12817765	ID=MS.gene59408.t1.exon3;Parent=MS.gene59408.t1
chr5.4	CDS	12817765	12817977	12817765	ID=cds.MS.gene59408.t1;Parent=MS.gene59408.t1

Gene Sequence

>MS.gene59408

ATGGTCAAGAAAACTGTCTTGAAGGTTAATATCGATTGTCCCAAATGCAAAAAGAAACTCATCAAAACTGTCTCTTCTCTTGAAGGTAACCACAGCATTTAGATTATACACAACCTACATTAGTACTTAATCTCCTTCTCCTCTCTCACTATTTTTGTTATGGATTGAATTAACATTTAAAATGGTTATTGCTTGCTATAAAGATTTTGAGTAGAAACATATGTTGTGAATTTTTTTCATTGAATTGCAGGGATAGATAAAATTGAAGCTGATGAAGTGAAGGGAACATTGACAATATTAGGGGATGCAGACCCATATGACATAATAGTTCGGATAAGGAAAGCAGGGAAACATGCTGAAGTTGTGAGTATAGGGCCTCCCCCAGGCCCTGCCCCTCCACCTGGCCCGGCGCCTCCGAAACAGGATGCTGCAAAAAAGCCTGCAGAAAATAAAAAGCCCGAAGAAAAGAGTAAGCCTGGTGATAAGGATAAGCCCAGCCCAGTGAGTTTGGACCAAATATCTTATGTGCCCTATACGCAGATGCCTTATTACTACCCACAGTACCAGGGCCAGCCAGTTGCTGTGGTGCACATGAATAGGTGGGATGAACCCAATACATCTTGCATTATTATGTGA

Protein sequence

>MS.gene59408.t1

MVKKTVLKVNIDCPKCKKKLIKTVSSLEGIDKIEADEVKGTLTILGDADPYDIIVRIRKAGKHAEVDAAKKPAENKKPEEKSKPGDKDKPSPVSLDQISYVPYTQMPYYYPQYQGQPVAVVHMNRWDEPNTSCIIM