AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59522

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59522.t1 MTR_8g027495 96.951 328 10 0 151 478 296 623 0.0 614
MS.gene59522.t1 MTR_8g027495 98.684 152 2 0 1 152 20 171 1.55e-104 325
MS.gene59522.t1 MTR_7g117670 61.721 337 84 12 156 478 262 567 6.27e-107 329
MS.gene59522.t1 MTR_7g117670 58.434 166 54 4 1 152 16 180 1.52e-52 186
MS.gene59522.t1 MTR_7g096350 54.922 193 65 5 286 473 327 502 1.53e-56 196
MS.gene59522.t1 MTR_7g096350 30.061 163 83 7 1 152 15 157 5.51e-13 71.2
MS.gene59522.t1 MTR_8g067280 44.240 217 98 5 278 472 424 639 2.21e-44 165
MS.gene59522.t1 MTR_5g030430 42.793 222 102 4 274 472 451 670 7.83e-43 161
MS.gene59522.t1 MTR_5g030430 38.017 121 68 3 32 152 43 156 1.11e-12 70.9
MS.gene59522.t1 MTR_8g024790 38.953 172 96 1 293 455 271 442 7.69e-34 133
MS.gene59522.t1 MTR_8g024770 41.270 189 97 4 293 468 259 446 1.31e-33 132
MS.gene59522.t1 MTR_4g092700 36.923 130 67 3 311 425 156 285 1.55e-16 80.9
MS.gene59522.t1 MTR_2g010450 31.618 136 84 2 313 439 177 312 2.31e-16 80.9
MS.gene59522.t1 MTR_5g014640 39.252 107 60 2 314 415 155 261 8.95e-16 78.6
MS.gene59522.t1 MTR_5g014600 29.730 148 83 3 313 439 149 296 2.70e-15 77.4
MS.gene59522.t1 MTR_8g098275 51.389 72 35 0 310 381 241 312 3.76e-15 77.8
MS.gene59522.t1 MTR_8g098275 51.389 72 35 0 310 381 305 376 5.48e-15 77.8
MS.gene59522.t1 MTR_2g104550 29.221 154 81 3 290 415 96 249 5.52e-15 76.3
MS.gene59522.t1 MTR_8g098275 28.500 200 106 4 310 473 398 596 5.71e-15 77.8
MS.gene59522.t1 MTR_4g067010 30.286 175 101 3 292 458 136 297 9.69e-15 75.5
MS.gene59522.t1 MTR_3g498695 35.036 137 71 2 294 420 287 415 1.42e-14 76.3
MS.gene59522.t1 MTR_3g498695 35.036 137 71 2 294 420 287 415 1.48e-14 76.3
MS.gene59522.t1 MTR_1g072320 55.000 60 27 0 313 372 470 529 1.61e-14 76.6
MS.gene59522.t1 MTR_4g097950 35.537 121 63 2 313 418 70 190 2.60e-14 73.2
MS.gene59522.t1 MTR_1g112680 41.489 94 49 2 278 371 288 375 2.72e-14 75.5
MS.gene59522.t1 MTR_6g047550 51.562 64 31 0 313 376 173 236 4.15e-14 72.8
MS.gene59522.t1 MTR_0250s0040 44.318 88 44 2 290 377 70 152 4.46e-14 72.8
MS.gene59522.t1 MTR_2g084280 27.011 174 113 4 293 460 121 286 4.52e-14 73.6
MS.gene59522.t1 MTR_5g014520 27.922 154 103 1 313 458 157 310 6.72e-14 73.2
MS.gene59522.t1 MTR_4g097940 34.146 123 62 3 301 413 147 260 6.92e-14 73.2
MS.gene59522.t1 MTR_2g038040 29.268 164 95 4 314 456 53 216 7.49e-14 71.6
MS.gene59522.t1 MTR_0246s0020 44.872 78 37 1 299 376 190 261 7.58e-14 73.2
MS.gene59522.t1 MTR_0246s0020 44.872 78 37 1 299 376 190 261 8.74e-14 73.2
MS.gene59522.t1 MTR_4g066380 46.753 77 31 1 292 368 142 208 9.97e-14 72.4
MS.gene59522.t1 MTR_4g057270 32.353 170 100 7 317 471 153 322 1.13e-13 72.4
MS.gene59522.t1 MTR_4g066460 46.753 77 31 1 292 368 144 210 2.20e-13 71.6
MS.gene59522.t1 MTR_0011s0210 29.885 174 107 3 313 474 183 353 2.81e-13 71.6
MS.gene59522.t1 MTR_3g030700 26.131 199 133 3 293 477 176 374 2.83e-13 71.6
MS.gene59522.t1 MTR_6g047570 29.221 154 93 2 313 454 159 308 3.14e-13 71.2
MS.gene59522.t1 MTR_0011s0260 28.736 174 109 3 313 474 183 353 3.87e-13 71.2
MS.gene59522.t1 MTR_7g083900 53.333 60 28 0 311 370 284 343 4.83e-13 71.2
MS.gene59522.t1 MTR_7g083900 53.333 60 28 0 311 370 284 343 4.89e-13 71.2
MS.gene59522.t1 MTR_5g014560 26.744 172 103 3 315 470 133 297 1.13e-12 68.9
MS.gene59522.t1 MTR_2g104490 31.405 121 76 3 301 415 126 245 2.01e-12 68.6
MS.gene59522.t1 MTR_2g104490 31.405 121 76 3 301 415 126 245 2.20e-12 68.2
MS.gene59522.t1 MTR_4g009110 38.961 77 43 1 299 375 164 236 3.15e-12 68.2
MS.gene59522.t1 MTR_4g097920 48.276 58 30 0 309 366 136 193 5.93e-12 67.0
MS.gene59522.t1 MTR_3g104100 25.568 176 112 5 317 473 199 374 6.11e-12 67.4
MS.gene59522.t1 MTR_2g104560 24.419 172 123 2 314 478 55 226 6.68e-12 65.5
MS.gene59522.t1 MTR_4g097920 48.276 58 30 0 309 366 151 208 6.92e-12 67.0
MS.gene59522.t1 MTR_4g009540 27.820 133 74 3 299 415 142 268 1.00e-11 66.2
MS.gene59522.t1 MTR_4g009540 27.820 133 74 3 299 415 142 268 1.21e-11 66.2
MS.gene59522.t1 MTR_2g081040 24.432 176 114 4 317 473 181 356 1.27e-11 66.2
MS.gene59522.t1 MTR_5g030420 35.537 121 71 3 32 152 26 139 1.74e-11 64.7
MS.gene59522.t1 MTR_5g030420 35.537 121 71 3 32 152 26 139 1.86e-11 64.7
MS.gene59522.t1 MTR_8g009010 31.452 124 85 0 245 368 66 189 2.46e-11 65.1
MS.gene59522.t1 MTR_2g022280 24.294 177 113 3 312 467 1 177 8.09e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59522.t1 AT4G16430 35.363 509 238 19 1 473 14 467 8.07e-77 248
MS.gene59522.t1 AT2G46510 43.529 340 126 10 157 477 258 550 2.06e-71 237
MS.gene59522.t1 AT2G46510 55.625 160 59 2 1 152 15 170 4.90e-49 177
MS.gene59522.t1 AT1G01260 42.818 369 141 14 152 478 242 582 2.76e-71 237
MS.gene59522.t1 AT1G01260 67.320 153 49 1 1 152 15 167 6.16e-63 215
MS.gene59522.t1 AT1G01260 42.818 369 141 14 152 478 242 582 2.76e-71 237
MS.gene59522.t1 AT1G01260 67.320 153 49 1 1 152 15 167 6.16e-63 215
MS.gene59522.t1 AT1G01260 42.818 369 141 14 152 478 242 582 2.76e-71 237
MS.gene59522.t1 AT1G01260 67.320 153 49 1 1 152 15 167 6.16e-63 215
MS.gene59522.t1 AT5G46760 44.954 218 104 5 268 471 369 584 1.09e-46 171
MS.gene59522.t1 AT1G32640 45.933 209 100 3 272 471 414 618 1.21e-45 168
MS.gene59522.t1 AT1G32640 35.833 120 72 3 32 150 66 181 4.17e-11 65.9
MS.gene59522.t1 AT4G17880 42.056 214 111 4 274 475 373 585 1.95e-44 164
MS.gene59522.t1 AT4G17880 35.156 128 69 3 32 152 61 181 6.60e-12 68.2
MS.gene59522.t1 AT5G46830 31.195 452 254 12 52 455 49 491 7.44e-40 150
MS.gene59522.t1 AT5G57150 32.796 186 102 5 293 461 40 219 4.90e-20 89.7
MS.gene59522.t1 AT4G00870 50.549 91 35 1 298 378 224 314 6.45e-20 92.4
MS.gene59522.t1 AT5G57150 31.667 180 100 4 293 455 40 213 2.06e-19 87.4
MS.gene59522.t1 AT5G57150 31.667 180 100 4 293 455 41 214 2.93e-19 87.0
MS.gene59522.t1 AT5G57150 33.333 159 89 3 314 455 56 214 3.07e-19 87.8
MS.gene59522.t1 AT5G57150 33.333 159 89 3 314 455 56 214 3.08e-19 86.7
MS.gene59522.t1 AT4G37850 29.648 199 120 3 280 463 119 312 7.00e-17 82.0
MS.gene59522.t1 AT4G37850 29.648 199 120 3 280 463 119 312 7.00e-17 82.0
MS.gene59522.t1 AT4G29930 29.412 170 103 4 297 460 43 201 7.90e-16 77.0
MS.gene59522.t1 AT4G29930 28.659 164 102 4 297 455 43 196 9.72e-16 77.0
MS.gene59522.t1 AT4G29930 28.144 167 112 3 297 460 43 204 1.36e-15 76.6
MS.gene59522.t1 AT4G29930 58.621 58 24 0 315 372 56 113 2.97e-14 71.6
MS.gene59522.t1 AT4G09820 45.570 79 32 1 293 371 354 421 3.12e-14 75.5
MS.gene59522.t1 AT4G29930 48.684 76 34 1 297 372 43 113 4.26e-14 72.8
MS.gene59522.t1 AT4G29930 48.684 76 34 1 297 372 43 113 4.26e-14 72.8
MS.gene59522.t1 AT4G29930 48.684 76 34 1 297 372 43 113 4.49e-14 72.4
MS.gene59522.t1 AT2G22750 36.111 144 57 4 296 415 133 265 7.05e-14 73.2
MS.gene59522.t1 AT2G22750 36.111 144 57 4 296 415 137 269 7.72e-14 73.2
MS.gene59522.t1 AT2G22750 36.111 144 57 4 296 415 109 241 7.74e-14 72.8
MS.gene59522.t1 AT2G22750 36.111 144 57 4 296 415 137 269 8.00e-14 72.8
MS.gene59522.t1 AT2G22750 36.111 144 57 4 296 415 109 241 8.56e-14 72.4
MS.gene59522.t1 AT3G26744 37.500 120 65 1 311 420 305 424 8.82e-14 73.9
MS.gene59522.t1 AT3G26744 37.500 120 65 1 311 420 305 424 1.04e-13 73.6
MS.gene59522.t1 AT3G26744 37.500 120 65 1 311 420 305 424 1.04e-13 73.6
MS.gene59522.t1 AT3G26744 37.500 120 65 1 311 420 305 424 1.04e-13 73.6
MS.gene59522.t1 AT1G12860 49.333 75 34 1 311 385 265 335 1.35e-13 73.2
MS.gene59522.t1 AT1G12860 49.333 75 34 1 311 385 265 335 1.38e-13 73.2
MS.gene59522.t1 AT2G16910 28.022 182 99 3 317 467 318 498 1.46e-13 73.6
MS.gene59522.t1 AT2G22760 33.333 117 59 3 314 415 120 232 1.50e-13 71.6
MS.gene59522.t1 AT2G22760 33.333 117 59 3 314 415 120 232 1.51e-13 71.6
MS.gene59522.t1 AT1G63650 47.692 65 34 0 313 377 405 469 1.94e-13 73.2
MS.gene59522.t1 AT1G63650 47.692 65 34 0 313 377 405 469 1.94e-13 73.2
MS.gene59522.t1 AT1G63650 47.692 65 34 0 313 377 405 469 1.94e-13 73.2
MS.gene59522.t1 AT5G41315 45.070 71 39 0 307 377 443 513 1.15e-12 70.9
MS.gene59522.t1 AT5G41315 45.070 71 39 0 307 377 440 510 1.22e-12 70.5
MS.gene59522.t1 AT5G41315 45.070 71 39 0 307 377 435 505 1.26e-12 70.5
MS.gene59522.t1 AT2G28160 30.380 158 96 5 317 461 135 291 1.65e-12 68.6
MS.gene59522.t1 AT2G28160 30.380 158 96 5 317 461 135 291 1.82e-12 68.6
MS.gene59522.t1 AT2G22770 27.536 207 116 5 280 455 95 298 3.70e-12 67.8
MS.gene59522.t1 AT1G10610 26.452 155 102 3 314 456 265 419 6.43e-11 64.7
MS.gene59522.t1 AT1G10610 26.452 155 102 3 314 456 235 389 9.14e-11 64.3
MS.gene59522.t1 AT1G10610 26.452 155 102 3 314 456 168 322 9.28e-11 63.5

Find 104 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAAAAGGAACAGAATT+TGG 0.212083 8.4:-69253239 MS.gene59522:CDS
CTATAAGGAGTTGGTTTCTT+TGG 0.221769 8.4:+69251692 None:intergenic
GATGATGACAATTATGCTTT+TGG 0.241708 8.4:-69253130 MS.gene59522:CDS
CTGATGTTGAAGCTTCTTGC+AGG 0.272050 8.4:-69252274 MS.gene59522:CDS
GGTTCTACCCTTCCATTTGC+TGG 0.277893 8.4:+69252197 None:intergenic
TGAACTCGGCCACACAATTT+CGG 0.285202 8.4:-69252610 MS.gene59522:CDS
GAAACTTGCTATTAGGAAAA+TGG 0.287769 8.4:-69252585 MS.gene59522:CDS
TTCGGGAGAAACTTGCTATT+AGG 0.289104 8.4:-69252592 MS.gene59522:CDS
GAACTCGGCCACACAATTTC+GGG 0.292031 8.4:-69252609 MS.gene59522:CDS
CGAATTTCTCAGTCAAAGTT+TGG 0.303426 8.4:-69253310 MS.gene59522:CDS
TTGTCCATCTTGGAAATATT+CGG 0.320643 8.4:+69252098 None:intergenic
GAGGAAAAGGAACAGAATTT+GGG 0.321673 8.4:-69253238 MS.gene59522:CDS
CAAAGATGCTTTGTCCATCT+TGG 0.334458 8.4:+69252088 None:intergenic
CAGAAGCTTCATGCAACATT+TGG 0.335516 8.4:-69253160 MS.gene59522:CDS
CGACTCAACGATATCGATTT+CGG 0.339702 8.4:+69251812 None:intergenic
CTTGAACCCTGAACACCATT+TGG 0.342953 8.4:+69252512 None:intergenic
TCAGTCAAAGTTTGGTGATT+GGG 0.345613 8.4:-69253302 MS.gene59522:CDS
CAAATCCTGCCCGAAAATCT+TGG 0.349356 8.4:+69252631 None:intergenic
CCTCCTATCATCGGAGGGAC+TGG 0.349816 8.4:+69252241 None:intergenic
GTTGGGGAGATGGGTGTTGT+AGG 0.351229 8.4:-69253273 MS.gene59522:CDS
CTCAGTCAAAGTTTGGTGAT+TGG 0.372558 8.4:-69253303 MS.gene59522:CDS
TTTCGGTGTCTTTGAATGAT+TGG 0.372935 8.4:+69251829 None:intergenic
CCTGGTCCACCATGTCCATT+AGG 0.373360 8.4:+69253049 None:intergenic
GGCTTTCTGCCACGTTTCCT+TGG 0.373396 8.4:+69252218 None:intergenic
CCTCCCACCGTTTGCTAGCT+CGG 0.375137 8.4:+69252412 None:intergenic
GAATATGAACTATGGCTATA+AGG 0.379179 8.4:+69251677 None:intergenic
ATGGACAAAGCATCTTTGTT+AGG 0.382051 8.4:-69252083 MS.gene59522:CDS
CATGGTAGAGTTGAGGGGAT+TGG 0.398380 8.4:-69252659 MS.gene59522:intron
CTCTGCCTCCACATGATTGA+GGG 0.404175 8.4:+69252175 None:intergenic
GAATCCGAGAGAGAGCAATT+AGG 0.414650 8.4:-69252011 MS.gene59522:CDS
AGCAACAAAGAATGAGGAAA+AGG 0.416440 8.4:-69253189 MS.gene59522:CDS
TCTCTGCCTCCACATGATTG+AGG 0.421326 8.4:+69252174 None:intergenic
GCAGCTTGAATATCGATATC+AGG 0.436804 8.4:+69251915 None:intergenic
GAGATCAATTGTTGGTGAAT+CGG 0.451925 8.4:-69252303 MS.gene59522:CDS
AAATCCTGCCCGAAAATCTT+GGG 0.454281 8.4:+69252632 None:intergenic
CTCCTATCATCGGAGGGACT+GGG 0.458724 8.4:+69252242 None:intergenic
ATCCCAGTCCCTCCGATGAT+AGG 0.460893 8.4:-69252244 MS.gene59522:CDS
CCTAATGGACATGGTGGACC+AGG 0.463512 8.4:-69253049 MS.gene59522:intron
CGTGGCAGAAAGCCAGCAAA+TGG 0.467789 8.4:-69252209 MS.gene59522:CDS
CTATTAGGAAAATGGATGAT+AGG 0.467799 8.4:-69252577 MS.gene59522:CDS
CATGGGGAGGCCGTCCGAAT+GGG 0.483663 8.4:-69252550 MS.gene59522:CDS
GAACTATGGCTATAAGGAGT+TGG 0.485125 8.4:+69251683 None:intergenic
AAGCTTCATGCAACATTTGG+TGG 0.485880 8.4:-69253157 MS.gene59522:CDS
CTAACGTTTCGAATTTCAGT+TGG 0.492616 8.4:-69253351 MS.gene59522:CDS
AAATGGATGATAGGCAATCA+TGG 0.495557 8.4:-69252568 MS.gene59522:CDS
CCTTCAGTGAGATCAATTGT+TGG 0.497365 8.4:-69252311 MS.gene59522:CDS
GGAGGCCGTCCGAATGGGAA+TGG 0.498948 8.4:-69252545 MS.gene59522:CDS
TTTCCTTGGCCTCCTATCAT+CGG 0.501052 8.4:+69252232 None:intergenic
AAACTAATTCCATTCCCATT+CGG 0.503424 8.4:+69252536 None:intergenic
TGATGTTGAAGCTTCTTGCA+GGG 0.510338 8.4:-69252273 MS.gene59522:CDS
GGAACCTAAAGAGGAGGAAA+AGG 0.510397 8.4:-69253251 MS.gene59522:CDS
TCATGGGGAGGCCGTCCGAA+TGG 0.512768 8.4:-69252551 MS.gene59522:CDS
CTTCTCCTTTGTCAGCTGCT+CGG 0.521163 8.4:+69251731 None:intergenic
TTGGGGAGATGGGTGTTGTA+GGG 0.522649 8.4:-69253272 MS.gene59522:CDS
GCATTGCTCGATGAAGGCCT+AGG 0.524340 8.4:+69252437 None:intergenic
GCAACAAAGAATGAGGAAAA+GGG 0.524452 8.4:-69253188 MS.gene59522:CDS
GATTCCTAATTGCTCTCTCT+CGG 0.526835 8.4:+69252007 None:intergenic
TTAGGAATCGCTTCTAGAGA+TGG 0.528320 8.4:-69251993 MS.gene59522:CDS
GATATTCAAGCTGCACAAGA+TGG 0.542476 8.4:-69251906 MS.gene59522:CDS
ATGAAAAGCAACAAAGAATG+AGG 0.547021 8.4:-69253195 MS.gene59522:CDS
AATGCAGCCGAGCTAGCAAA+CGG 0.548916 8.4:-69252419 MS.gene59522:CDS
TCGGCTGCATTGCTCGATGA+AGG 0.551061 8.4:+69252431 None:intergenic
AATGGATGATAGGCAATCAT+GGG 0.552039 8.4:-69252567 MS.gene59522:CDS
CCGAGCTAGCAAACGGTGGG+AGG 0.554145 8.4:-69252412 MS.gene59522:CDS
TGTGCCGAATATTTCCAAGA+TGG 0.560772 8.4:-69252102 MS.gene59522:CDS
ATTGGACCGTGTGACCGATA+CGG 0.561637 8.4:-69253107 MS.gene59522:CDS
CTTGGCCTCCTATCATCGGA+GGG 0.566577 8.4:+69252236 None:intergenic
GCAGAAAGCCAGCAAATGGA+AGG 0.566807 8.4:-69252205 MS.gene59522:CDS
CCTTGGCCTCCTATCATCGG+AGG 0.567260 8.4:+69252235 None:intergenic
TTCTAGAGATGGATCATCAT+CGG 0.575066 8.4:-69251982 MS.gene59522:CDS
CCAGTCCCTCCGATGATAGG+AGG 0.584284 8.4:-69252241 MS.gene59522:CDS
CAGCCGAGCTAGCAAACGGT+GGG 0.586818 8.4:-69252415 MS.gene59522:CDS
TTTCGGGCAGGATTTGAACT+CGG 0.588089 8.4:-69252624 MS.gene59522:CDS
ATGGTGTTCAGGGTTCAAGT+TGG 0.593722 8.4:-69252508 MS.gene59522:CDS
TCTGCCTCCACATGATTGAG+GGG 0.599597 8.4:+69252176 None:intergenic
GTGTTGTAGGGAACCTAAAG+AGG 0.602831 8.4:-69253260 MS.gene59522:CDS
TAATTCCATTCCCATTCGGA+CGG 0.603735 8.4:+69252540 None:intergenic
AAGTTTCTCCCGAAATTGTG+TGG 0.607085 8.4:+69252601 None:intergenic
AAGAAAGAACATCTCCGTAT+CGG 0.609322 8.4:+69253093 None:intergenic
CCTCCGATGATAGGAGGCCA+AGG 0.615234 8.4:-69252235 MS.gene59522:CDS
AGGTTCCGAGCAGCTGACAA+AGG 0.619745 8.4:-69251736 MS.gene59522:CDS
GTTCAGGGTTCAAGTTGGAG+AGG 0.625845 8.4:-69252503 MS.gene59522:CDS
ACAAGATGGAGTTATCGTAA+AGG 0.628284 8.4:-69251892 MS.gene59522:CDS
CCAACAATTGATCTCACTGA+AGG 0.629626 8.4:+69252311 None:intergenic
TCAAGCAAAACTCAAGACGA+TGG 0.633732 8.4:-69252033 MS.gene59522:CDS
AAGCTCGTTGATGTAAGCAA+TGG 0.643072 8.4:+69252055 None:intergenic
GGTAGAACCCCTCAATCATG+TGG 0.644553 8.4:-69252183 MS.gene59522:CDS
GTGACTTAATTATGAATGTG+TGG 0.646250 8.4:+69251760 None:intergenic
AGGAAAAGGAACAGAATTTG+GGG 0.646477 8.4:-69253237 MS.gene59522:CDS
ACATCTCCGTATCGGTCACA+CGG 0.649933 8.4:+69253101 None:intergenic
GATAGGAGGCCAAGGAAACG+TGG 0.651587 8.4:-69252227 MS.gene59522:CDS
ATAATCATGGTAGAGTTGAG+GGG 0.664095 8.4:-69252664 MS.gene59522:intron
TCAACAACTATCAACAGCAA+CGG 0.665554 8.4:-69252376 MS.gene59522:CDS
GGAGAGGTAGTCAAGTTGTG+AGG 0.666503 8.4:-69252487 MS.gene59522:CDS
GCAGCCGAGCTAGCAAACGG+TGG 0.666556 8.4:-69252416 MS.gene59522:CDS
TTGTAGGGAACCTAAAGAGG+AGG 0.672145 8.4:-69253257 MS.gene59522:CDS
TGTTGAAGCTTCTTGCAGGG+AGG 0.673213 8.4:-69252270 MS.gene59522:CDS
TCAATCATGTGGAGGCAGAG+AGG 0.677489 8.4:-69252172 MS.gene59522:CDS
AGAACCCCTCAATCATGTGG+AGG 0.677611 8.4:-69252180 MS.gene59522:CDS
GATGATAGGCAATCATGGGG+AGG 0.677666 8.4:-69252563 MS.gene59522:CDS
CAGAAAGCCAGCAAATGGAA+GGG 0.688128 8.4:-69252204 MS.gene59522:CDS
ACATTCATAATTAAGTCACA+AGG 0.691255 8.4:-69251756 MS.gene59522:CDS
TCTCACTGAAGGTCTCGATG+TGG 0.695158 8.4:+69252322 None:intergenic
ATGGATGATAGGCAATCATG+GGG 0.703008 8.4:-69252566 MS.gene59522:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAACAATAGTAATAATAA+TGG - chr8.4:69252460-69252479 MS.gene59522:CDS 15.0%
!!! TGTTAATGCTTTTGATTATT+TGG - chr8.4:69251712-69251731 MS.gene59522:CDS 20.0%
! ACATTCATAATTAAGTCACA+AGG - chr8.4:69253420-69253439 MS.gene59522:CDS 25.0%
! CATCAGAATAATGATAATCA+TGG - chr8.4:69252499-69252518 MS.gene59522:CDS 25.0%
! GAAAAATCAATTTGCATTTG+TGG + chr8.4:69252829-69252848 None:intergenic 25.0%
!! ATGTATTTCTCTTTTCCTAA+TGG - chr8.4:69252112-69252131 MS.gene59522:CDS 25.0%
!!! AATGGAATTAGTTTTCCAAA+TGG - chr8.4:69252649-69252668 MS.gene59522:CDS 25.0%
!!! CAAATGCAAATTGATTTTTC+AGG - chr8.4:69252829-69252848 MS.gene59522:intron 25.0%
!!! CTTTTTCTTGTTTGAAAGTT+GGG - chr8.4:69252428-69252447 MS.gene59522:CDS 25.0%
!!! TTTTTCTTGTTTGAAAGTTG+GGG - chr8.4:69252429-69252448 MS.gene59522:CDS 25.0%
AAACTAATTCCATTCCCATT+CGG + chr8.4:69252643-69252662 None:intergenic 30.0%
ATGAAAAGCAACAAAGAATG+AGG - chr8.4:69251981-69252000 MS.gene59522:CDS 30.0%
CCAACTTTCAAACAAGAAAA+AGG + chr8.4:69252430-69252449 None:intergenic 30.0%
CTATTAGGAAAATGGATGAT+AGG - chr8.4:69252599-69252618 MS.gene59522:CDS 30.0%
GTGACTTAATTATGAATGTG+TGG + chr8.4:69253419-69253438 None:intergenic 30.0%
TTGCTCTGATTATTGTGTTA+GGG - chr8.4:69252204-69252223 MS.gene59522:CDS 30.0%
TTGTCCATCTTGGAAATATT+CGG + chr8.4:69253081-69253100 None:intergenic 30.0%
! AGAATCTTTTGACAGCAATT+GGG - chr8.4:69251756-69251775 MS.gene59522:CDS 30.0%
! GATGATGACAATTATGCTTT+TGG - chr8.4:69252046-69252065 MS.gene59522:CDS 30.0%
! TGACAATTATGCTTTTGGAT+TGG - chr8.4:69252051-69252070 MS.gene59522:CDS 30.0%
!! CCTTTTTCTTGTTTGAAAGT+TGG - chr8.4:69252427-69252446 MS.gene59522:CDS 30.0%
!! GAAACTTGCTATTAGGAAAA+TGG - chr8.4:69252591-69252610 MS.gene59522:CDS 30.0%
!! GATTTAGGTGTTGTTGAATT+GGG - chr8.4:69252277-69252296 MS.gene59522:CDS 30.0%
!! TGATTTAGGTGTTGTTGAAT+TGG - chr8.4:69252276-69252295 MS.gene59522:CDS 30.0%
!!! GTTGGAATTATGCGATTTTT+TGG - chr8.4:69251843-69251862 MS.gene59522:CDS 30.0%
!!! GTTTTAGTTCCTACTGATTT+AGG - chr8.4:69252262-69252281 MS.gene59522:CDS 30.0%
!!! TTATTGTGTTAGGGCTTTTT+TGG - chr8.4:69252213-69252232 MS.gene59522:CDS 30.0%
AAATGGATGATAGGCAATCA+TGG - chr8.4:69252608-69252627 MS.gene59522:CDS 35.0%
AAGAAAGAACATCTCCGTAT+CGG + chr8.4:69252086-69252105 None:intergenic 35.0%
AATGGATGATAGGCAATCAT+GGG - chr8.4:69252609-69252628 MS.gene59522:CDS 35.0%
ACAAGATGGAGTTATCGTAA+AGG - chr8.4:69253284-69253303 MS.gene59522:CDS 35.0%
AGCAACAAAGAATGAGGAAA+AGG - chr8.4:69251987-69252006 MS.gene59522:CDS 35.0%
AGGAAAAGGAACAGAATTTG+GGG - chr8.4:69251939-69251958 MS.gene59522:CDS 35.0%
ATAATCATGGTAGAGTTGAG+GGG - chr8.4:69252512-69252531 MS.gene59522:CDS 35.0%
CGAATTTCTCAGTCAAAGTT+TGG - chr8.4:69251866-69251885 MS.gene59522:CDS 35.0%
CTAACGTTTCGAATTTCAGT+TGG - chr8.4:69251825-69251844 MS.gene59522:CDS 35.0%
GAGGAAAAGGAACAGAATTT+GGG - chr8.4:69251938-69251957 MS.gene59522:CDS 35.0%
GATAATCATGGTAGAGTTGA+GGG - chr8.4:69252511-69252530 MS.gene59522:CDS 35.0%
GCAACAAAGAATGAGGAAAA+GGG - chr8.4:69251988-69252007 MS.gene59522:CDS 35.0%
GTTGCTCTGATTATTGTGTT+AGG - chr8.4:69252203-69252222 MS.gene59522:CDS 35.0%
TCAACAACACCTAAATCAGT+AGG + chr8.4:69252274-69252293 None:intergenic 35.0%
TCAACAACTATCAACAGCAA+CGG - chr8.4:69252800-69252819 MS.gene59522:intron 35.0%
TGAATTGGGTTCTGTTAGAA+CGG - chr8.4:69252291-69252310 MS.gene59522:CDS 35.0%
TGATAATCATGGTAGAGTTG+AGG - chr8.4:69252510-69252529 MS.gene59522:CDS 35.0%
! AACTCAATCATCGTTTGCTA+GGG - chr8.4:69252360-69252379 MS.gene59522:CDS 35.0%
! AGTTTTCCAAATGGTGTTCA+GGG - chr8.4:69252658-69252677 MS.gene59522:intron 35.0%
! ATGGACAAAGCATCTTTGTT+AGG - chr8.4:69253093-69253112 MS.gene59522:CDS 35.0%
! ATTGGAGTTCCCAAGATTTT+CGG - chr8.4:69252535-69252554 MS.gene59522:CDS 35.0%
! CTATAAGGAGTTGGTTTCTT+TGG + chr8.4:69253487-69253506 None:intergenic 35.0%
! GAGAATCTTTTGACAGCAAT+TGG - chr8.4:69251755-69251774 MS.gene59522:CDS 35.0%
! GAGATCAATTGTTGGTGAAT+CGG - chr8.4:69252873-69252892 MS.gene59522:intron 35.0%
! TAGTTTTCCAAATGGTGTTC+AGG - chr8.4:69252657-69252676 MS.gene59522:intron 35.0%
! TCAGTCAAAGTTTGGTGATT+GGG - chr8.4:69251874-69251893 MS.gene59522:CDS 35.0%
! TTCTAGAGATGGATCATCAT+CGG - chr8.4:69253194-69253213 MS.gene59522:CDS 35.0%
!! AAGTTTGGTGATTGGGTTTT+AGG - chr8.4:69251881-69251900 MS.gene59522:CDS 35.0%
!! TTCTCTTTTCCTAATGGACA+TGG - chr8.4:69252118-69252137 MS.gene59522:CDS 35.0%
!! TTTCGGTGTCTTTGAATGAT+TGG + chr8.4:69253350-69253369 None:intergenic 35.0%
!!! GATTGGACGAGTTTTGAAAT+AGG + chr8.4:69253333-69253352 None:intergenic 35.0%
!!! GCTTTTTTGGCTAAATCTGT+TGG - chr8.4:69252226-69252245 MS.gene59522:CDS 35.0%
AAATCCTGCCCGAAAATCTT+GGG + chr8.4:69252547-69252566 None:intergenic 40.0%
AAGCTCGTTGATGTAAGCAA+TGG + chr8.4:69253124-69253143 None:intergenic 40.0%
AAGCTTCATGCAACATTTGG+TGG - chr8.4:69252019-69252038 MS.gene59522:CDS 40.0%
AAGTTTCTCCCGAAATTGTG+TGG + chr8.4:69252578-69252597 None:intergenic 40.0%
ATGGATGATAGGCAATCATG+GGG - chr8.4:69252610-69252629 MS.gene59522:CDS 40.0%
CAAAGATGCTTTGTCCATCT+TGG + chr8.4:69253091-69253110 None:intergenic 40.0%
CAACTCAATCATCGTTTGCT+AGG - chr8.4:69252359-69252378 MS.gene59522:CDS 40.0%
CAGAAGCTTCATGCAACATT+TGG - chr8.4:69252016-69252035 MS.gene59522:CDS 40.0%
CCAACAATTGATCTCACTGA+AGG + chr8.4:69252868-69252887 None:intergenic 40.0%
CCTTCAGTGAGATCAATTGT+TGG - chr8.4:69252865-69252884 MS.gene59522:intron 40.0%
GATATTCAAGCTGCACAAGA+TGG - chr8.4:69253270-69253289 MS.gene59522:CDS 40.0%
GATTCCTAATTGCTCTCTCT+CGG + chr8.4:69253172-69253191 None:intergenic 40.0%
GCAGCTTGAATATCGATATC+AGG + chr8.4:69253264-69253283 None:intergenic 40.0%
GGAGGAAAAGGAACAGAATT+TGG - chr8.4:69251937-69251956 MS.gene59522:CDS 40.0%
TAATTCCATTCCCATTCGGA+CGG + chr8.4:69252639-69252658 None:intergenic 40.0%
TCAAGCAAAACTCAAGACGA+TGG - chr8.4:69253143-69253162 MS.gene59522:CDS 40.0%
TGATGTTGAAGCTTCTTGCA+GGG - chr8.4:69252903-69252922 MS.gene59522:intron 40.0%
TGTGCCGAATATTTCCAAGA+TGG - chr8.4:69253074-69253093 MS.gene59522:CDS 40.0%
! AAGTCGATTTCATCGTTGGA+TGG - chr8.4:69252385-69252404 MS.gene59522:CDS 40.0%
! CAGGTAAGTGTTTTGCATCT+GGG - chr8.4:69252146-69252165 MS.gene59522:CDS 40.0%
! CTCAGTCAAAGTTTGGTGAT+TGG - chr8.4:69251873-69251892 MS.gene59522:CDS 40.0%
! TGTTCCTTTTCCTCCTCTTT+AGG + chr8.4:69251932-69251951 None:intergenic 40.0%
! TTAGGAATCGCTTCTAGAGA+TGG - chr8.4:69253183-69253202 MS.gene59522:CDS 40.0%
! TTGGAGTTCCCAAGATTTTC+GGG - chr8.4:69252536-69252555 MS.gene59522:CDS 40.0%
!! CGACTCAACGATATCGATTT+CGG + chr8.4:69253367-69253386 None:intergenic 40.0%
!! TCTTTTCCTAATGGACATGG+TGG - chr8.4:69252121-69252140 MS.gene59522:CDS 40.0%
!! TTCGGGAGAAACTTGCTATT+AGG - chr8.4:69252584-69252603 MS.gene59522:CDS 40.0%
!!! TGGTGATTGGGTTTTAGGTT+GGG - chr8.4:69251886-69251905 MS.gene59522:CDS 40.0%
!!! TTGGTGATTGGGTTTTAGGT+TGG - chr8.4:69251885-69251904 MS.gene59522:CDS 40.0%
!!! TTTTCTGCTCTAGAGTTTGC+TGG + chr8.4:69253225-69253244 None:intergenic 40.0%
ATGGTGTTCAGGGTTCAAGT+TGG - chr8.4:69252668-69252687 MS.gene59522:intron 45.0%
CAAATCCTGCCCGAAAATCT+TGG + chr8.4:69252548-69252567 None:intergenic 45.0%
CAGAAAGCCAGCAAATGGAA+GGG - chr8.4:69252972-69252991 MS.gene59522:intron 45.0%
CCAGATGCAAAACACTTACC+TGG + chr8.4:69252148-69252167 None:intergenic 45.0%
CTAGGAGCAAAAACATCGAC+AGG + chr8.4:69252724-69252743 None:intergenic 45.0%
CTCGTAAAGCGTAAAAACGC+TGG + chr8.4:69253050-69253069 None:intergenic 45.0%
CTGATGTTGAAGCTTCTTGC+AGG - chr8.4:69252902-69252921 MS.gene59522:intron 45.0%
CTTGAACCCTGAACACCATT+TGG + chr8.4:69252667-69252686 None:intergenic 45.0%
GAATCCGAGAGAGAGCAATT+AGG - chr8.4:69253165-69253184 MS.gene59522:CDS 45.0%
GGAACCTAAAGAGGAGGAAA+AGG - chr8.4:69251925-69251944 MS.gene59522:CDS 45.0%
GTGTTGTAGGGAACCTAAAG+AGG - chr8.4:69251916-69251935 MS.gene59522:CDS 45.0%
TGAACTCGGCCACACAATTT+CGG - chr8.4:69252566-69252585 MS.gene59522:CDS 45.0%
TTGGGTTCTGTTAGAACGGT+TGG - chr8.4:69252295-69252314 MS.gene59522:CDS 45.0%
TTGTAGGGAACCTAAAGAGG+AGG - chr8.4:69251919-69251938 MS.gene59522:CDS 45.0%
TTTCCTTGGCCTCCTATCAT+CGG + chr8.4:69252947-69252966 None:intergenic 45.0%
TTTCGGGCAGGATTTGAACT+CGG - chr8.4:69252552-69252571 MS.gene59522:CDS 45.0%
! CCAGGTAAGTGTTTTGCATC+TGG - chr8.4:69252145-69252164 MS.gene59522:CDS 45.0%
! CTTTTGACAGCAATTGGGAG+TGG - chr8.4:69251761-69251780 MS.gene59522:CDS 45.0%
! GGCTAAGTCGATTTCATCGT+TGG - chr8.4:69252381-69252400 MS.gene59522:CDS 45.0%
!! CTGTCGATGTTTTTGCTCCT+AGG - chr8.4:69252722-69252741 MS.gene59522:intron 45.0%
!! TTGCATCTGGGAAGCATTTG+TGG - chr8.4:69252158-69252177 MS.gene59522:CDS 45.0%
!!! GGTGATTGGGTTTTAGGTTG+GGG - chr8.4:69251887-69251906 MS.gene59522:CDS 45.0%
AATGCAGCCGAGCTAGCAAA+CGG - chr8.4:69252757-69252776 MS.gene59522:intron 50.0%
AGAACCCCTCAATCATGTGG+AGG - chr8.4:69252996-69253015 MS.gene59522:intron 50.0%
ATTGGACCGTGTGACCGATA+CGG - chr8.4:69252069-69252088 MS.gene59522:CDS 50.0%
CATGGTAGAGTTGAGGGGAT+TGG - chr8.4:69252517-69252536 MS.gene59522:CDS 50.0%
CTCTGCCTCCACATGATTGA+GGG + chr8.4:69253004-69253023 None:intergenic 50.0%
CTTCTCCTTTGTCAGCTGCT+CGG + chr8.4:69253448-69253467 None:intergenic 50.0%
GAACTCGGCCACACAATTTC+GGG - chr8.4:69252567-69252586 MS.gene59522:CDS 50.0%
GATGATAGGCAATCATGGGG+AGG - chr8.4:69252613-69252632 MS.gene59522:CDS 50.0%
GCAGAAAGCCAGCAAATGGA+AGG - chr8.4:69252971-69252990 MS.gene59522:intron 50.0%
GGTAGAACCCCTCAATCATG+TGG - chr8.4:69252993-69253012 MS.gene59522:intron 50.0%
GGTTCTACCCTTCCATTTGC+TGG + chr8.4:69252982-69253001 None:intergenic 50.0%
TCAATCATGTGGAGGCAGAG+AGG - chr8.4:69253004-69253023 MS.gene59522:intron 50.0%
TCTCACTGAAGGTCTCGATG+TGG + chr8.4:69252857-69252876 None:intergenic 50.0%
TCTCTGCCTCCACATGATTG+AGG + chr8.4:69253005-69253024 None:intergenic 50.0%
TCTGCCTCCACATGATTGAG+GGG + chr8.4:69253003-69253022 None:intergenic 50.0%
TGTTGAAGCTTCTTGCAGGG+AGG - chr8.4:69252906-69252925 MS.gene59522:intron 50.0%
! ACATCTCCGTATCGGTCACA+CGG + chr8.4:69252078-69252097 None:intergenic 50.0%
! GGAGAGGTAGTCAAGTTGTG+AGG - chr8.4:69252689-69252708 MS.gene59522:intron 50.0%
! TTGGGGAGATGGGTGTTGTA+GGG - chr8.4:69251904-69251923 MS.gene59522:CDS 50.0%
!! AGTTCCCAAGATTTTCGGGC+AGG - chr8.4:69252540-69252559 MS.gene59522:CDS 50.0%
!! GTTCAGGGTTCAAGTTGGAG+AGG - chr8.4:69252673-69252692 MS.gene59522:intron 50.0%
!!! GGGTTTTAGGTTGGGGAGAT+GGG - chr8.4:69251894-69251913 MS.gene59522:CDS 50.0%
!!! TGGGTTTTAGGTTGGGGAGA+TGG - chr8.4:69251893-69251912 MS.gene59522:CDS 50.0%
AGGTTCCGAGCAGCTGACAA+AGG - chr8.4:69253440-69253459 MS.gene59522:CDS 55.0%
ATCCCAGTCCCTCCGATGAT+AGG - chr8.4:69252932-69252951 MS.gene59522:intron 55.0%
CCTGGTCCACCATGTCCATT+AGG + chr8.4:69252130-69252149 None:intergenic 55.0%
CGTGGCAGAAAGCCAGCAAA+TGG - chr8.4:69252967-69252986 MS.gene59522:intron 55.0%
CTCCTATCATCGGAGGGACT+GGG + chr8.4:69252937-69252956 None:intergenic 55.0%
CTTGGCCTCCTATCATCGGA+GGG + chr8.4:69252943-69252962 None:intergenic 55.0%
GATAGGAGGCCAAGGAAACG+TGG - chr8.4:69252949-69252968 MS.gene59522:intron 55.0%
GCATTGCTCGATGAAGGCCT+AGG + chr8.4:69252742-69252761 None:intergenic 55.0%
GGCTTTCTGCCACGTTTCCT+TGG + chr8.4:69252961-69252980 None:intergenic 55.0%
TCGGCTGCATTGCTCGATGA+AGG + chr8.4:69252748-69252767 None:intergenic 55.0%
! GTTGGGGAGATGGGTGTTGT+AGG - chr8.4:69251903-69251922 MS.gene59522:CDS 55.0%
!! CCTAATGGACATGGTGGACC+AGG - chr8.4:69252127-69252146 MS.gene59522:CDS 55.0%
CAGCCGAGCTAGCAAACGGT+GGG - chr8.4:69252761-69252780 MS.gene59522:intron 60.0%
CCAGTCCCTCCGATGATAGG+AGG - chr8.4:69252935-69252954 MS.gene59522:intron 60.0%
CCTCCGATGATAGGAGGCCA+AGG - chr8.4:69252941-69252960 MS.gene59522:intron 60.0%
CCTCCTATCATCGGAGGGAC+TGG + chr8.4:69252938-69252957 None:intergenic 60.0%
CCTTGGCCTCCTATCATCGG+AGG + chr8.4:69252944-69252963 None:intergenic 60.0%
! CCTCCCACCGTTTGCTAGCT+CGG + chr8.4:69252767-69252786 None:intergenic 60.0%
CATGGGGAGGCCGTCCGAAT+GGG - chr8.4:69252626-69252645 MS.gene59522:CDS 65.0%
CCGAGCTAGCAAACGGTGGG+AGG - chr8.4:69252764-69252783 MS.gene59522:intron 65.0%
GCAGCCGAGCTAGCAAACGG+TGG - chr8.4:69252760-69252779 MS.gene59522:intron 65.0%
GGAGGCCGTCCGAATGGGAA+TGG - chr8.4:69252631-69252650 MS.gene59522:CDS 65.0%
TCATGGGGAGGCCGTCCGAA+TGG - chr8.4:69252625-69252644 MS.gene59522:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 69251692 69253506 69251692 ID=MS.gene59522
chr8.4 mRNA 69251692 69253506 69251692 ID=MS.gene59522.t1;Parent=MS.gene59522
chr8.4 exon 69253050 69253506 69253050 ID=MS.gene59522.t1.exon1;Parent=MS.gene59522.t1
chr8.4 CDS 69253050 69253506 69253050 ID=cds.MS.gene59522.t1;Parent=MS.gene59522.t1
chr8.4 exon 69251692 69252671 69251692 ID=MS.gene59522.t1.exon2;Parent=MS.gene59522.t1
chr8.4 CDS 69251692 69252671 69251692 ID=cds.MS.gene59522.t1;Parent=MS.gene59522.t1
Gene Sequence

>MS.gene59522

ATGGTGGTTGAAGTTTTAGGTGTTAATGCTTTTGATTATTTGGTTACAAATTCAGTTTCAAATGAGAATCTTTTGACAGCAATTGGGAGTGGTGAGAATCTTGAAAACAAGCTTTCTGATCTAGTTGAAAGATCTAACGTTTCGAATTTCAGTTGGAATTATGCGATTTTTTGGCGAATTTCTCAGTCAAAGTTTGGTGATTGGGTTTTAGGTTGGGGAGATGGGTGTTGTAGGGAACCTAAAGAGGAGGAAAAGGAACAGAATTTGGGGATGAAAAGTGTTGTTGAAGATGAAAAGCAACAAAGAATGAGGAAAAGGGTGTTGCAGAAGCTTCATGCAACATTTGGTGGTTTAGATGATGACAATTATGCTTTTGGATTGGACCGTGTGACCGATACGGAGATGTTCTTTCTTGCATCAATGTATTTCTCTTTTCCTAATGGACATGGTGGACCAGGTAAGTGTTTTGCATCTGGGAAGCATTTGTGGTTATGTGATGCATTGAAATCTAGTTGCTCTGATTATTGTGTTAGGGCTTTTTTGGCTAAATCTGTTGGATTTAAGACTGTTGTTTTAGTTCCTACTGATTTAGGTGTTGTTGAATTGGGTTCTGTTAGAACGGTTGGTGAGAGCGTTGAATTGTTGCAAACTATTAAATCTGTGTTTTCAACTCAATCATCGTTTGCTAGGGCTAAGTCGATTTCATCGTTGGATGGAAAGAGAGATGAGAATGCTCCTTTTTCTTGTTTGAAAGTTGGGGAGAGTGTTAAGAACAATAGTAATAATAATGGTAATCTTCATCTTCATCATCAGAATAATGATAATCATGGTAGAGTTGAGGGGATTGGAGTTCCCAAGATTTTCGGGCAGGATTTGAACTCGGCCACACAATTTCGGGAGAAACTTGCTATTAGGAAAATGGATGATAGGCAATCATGGGGAGGCCGTCCGAATGGGAATGGAATTAGTTTTCCAAATGGTGTTCAGGGTTCAAGTTGGAGAGGTAGTCAAGTTGTGAGGCAGCATGCGCCTGTCGATGTTTTTGCTCCTAGGCCTTCATCGAGCAATGCAGCCGAGCTAGCAAACGGTGGGAGGCAAGATTTCGTGCTCAACAACTATCAACAGCAACGGAAGCCACAAATGCAAATTGATTTTTCAGGAGCCACATCGAGACCTTCAGTGAGATCAATTGTTGGTGAATCGGAGCTTTCTGATGTTGAAGCTTCTTGCAGGGAGGAGAATCCCAGTCCCTCCGATGATAGGAGGCCAAGGAAACGTGGCAGAAAGCCAGCAAATGGAAGGGTAGAACCCCTCAATCATGTGGAGGCAGAGAGGCAAAGACGCGAGAAGCTAAACCAGCGTTTTTACGCTTTACGAGCTGTTGTGCCGAATATTTCCAAGATGGACAAAGCATCTTTGTTAGGAGATGCCATTGCTTACATCAACGAGCTTCAAGCAAAACTCAAGACGATGGAATCCGAGAGAGAGCAATTAGGAATCGCTTCTAGAGATGGATCATCATCGGTATTGCCAGCAAACTCTAGAGCAGAAAATCATCAAAGCAGTGCTCCTGATATCGATATTCAAGCTGCACAAGATGGAGTTATCGTAAAGGTGAGTTGTTCTATTGATGTTCATCCTATTTCAAAACTCGTCCAATCATTCAAAGACACCGAAATCGATATCGTTGAGTCGAAACTCAATGCTGCAAATGATACTGTCTTCCACACATTCATAATTAAGTCACAAGGTTCCGAGCAGCTGACAAAGGAGAAGTTGATTGCAGCATTTTCCAAAGAAACCAACTCCTTATAG
Protein sequence

>MS.gene59522.t1

MVVEVLGVNAFDYLVTNSVSNENLLTAIGSGENLENKLSDLVERSNVSNFSWNYAIFWRISQSKFGDWVLGWGDGCCREPKEEEKEQNLGMKSVVEDEKQQRMRKRVLQKLHATFGGLDDDNYAFGLDRVTDTEMFFLASMYFSFPNGHGGPVEGIGVPKIFGQDLNSATQFREKLAIRKMDDRQSWGGRPNGNGISFPNGVQGSSWRGSQVVRQHAPVDVFAPRPSSSNAAELANGGRQDFVLNNYQQQRKPQMQIDFSGATSRPSVRSIVGESELSDVEASCREENPSPSDDRRPRKRGRKPANGRVEPLNHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAIAYINELQAKLKTMESEREQLGIASRDGSSSVLPANSRAENHQSSAPDIDIQAAQDGVIVKVSCSIDVHPISKLVQSFKDTEIDIVESKLNAANDTVFHTFIIKSQGSEQLTKEKLIAAFSKETNSL