Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59580.t1 | RHN39757.1 | 89.8 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 2.20E-63 | 251.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59580.t1 | A0A396GM00 | 89.8 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 1.6e-63 | 251.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052187 | MS.gene59580 | 0.804528 | 2.07E-49 | -1.69E-46 |
| MS.gene059632 | MS.gene59580 | 0.800592 | 1.34E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59580.t1 | MTR_8g027905 | 85.039 | 127 | 19 | 0 | 1 | 127 | 1 | 127 | 1.44e-76 | 227 |
| MS.gene59580.t1 | MTR_8g027305 | 90.411 | 73 | 7 | 0 | 1 | 73 | 1 | 73 | 2.12e-44 | 140 |
| MS.gene59580.t1 | MTR_8g027915 | 39.796 | 98 | 53 | 1 | 1 | 98 | 1 | 92 | 8.56e-14 | 64.3 |
| MS.gene59580.t1 | MTR_7g117120 | 44.643 | 56 | 31 | 0 | 18 | 73 | 8 | 63 | 6.89e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59580.t1 | AT3G61660 | 50.000 | 60 | 26 | 1 | 8 | 67 | 2 | 57 | 6.45e-14 | 64.7 |
| MS.gene59580.t1 | AT3G61660 | 60.976 | 41 | 16 | 0 | 27 | 67 | 7 | 47 | 2.26e-13 | 62.8 |
Find 19 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTGGGTCACAAGCTTT+CGG | 0.332858 | 8.3:+65867859 | MS.gene59580:CDS |
| AGTTCATATGAATCATATAA+AGG | 0.353897 | 8.3:-65867708 | None:intergenic |
| AGAAAGCAAAAGAGTCTTCT+TGG | 0.367922 | 8.3:+65867844 | MS.gene59580:CDS |
| AGAATTTGAAAAGGATGAAC+AGG | 0.368505 | 8.3:+65867617 | MS.gene59580:CDS |
| CTCTTGATCATCACATCAAC+AGG | 0.370624 | 8.3:+65867736 | MS.gene59580:CDS |
| TATTCATGATTCTCATGATA+TGG | 0.389972 | 8.3:+65867812 | MS.gene59580:CDS |
| TTTATATGATTCATATGAAC+TGG | 0.406641 | 8.3:+65867710 | MS.gene59580:CDS |
| GATGAAGATGATGAAGAGTA+AGG | 0.428689 | 8.3:+65867890 | MS.gene59580:CDS |
| GTTCATATGAATCATATAAA+GGG | 0.444445 | 8.3:-65867707 | None:intergenic |
| TCACATCAACAGGAATCTAA+TGG | 0.461388 | 8.3:+65867746 | MS.gene59580:CDS |
| GGATGAACAGGAAGCATTGA+AGG | 0.461606 | 8.3:+65867629 | MS.gene59580:CDS |
| ATGATGAAGAGTAAGGATAG+TGG | 0.486977 | 8.3:+65867897 | MS.gene59580:CDS |
| GAAAGCAAAAGAGTCTTCTT+GGG | 0.496086 | 8.3:+65867845 | MS.gene59580:CDS |
| GAGTGGAAAAGAATTTGAAA+AGG | 0.533590 | 8.3:+65867608 | MS.gene59580:CDS |
| CAAGGAAATGAGAAGAGGTA+TGG | 0.543597 | 8.3:+65867941 | MS.gene59580:CDS |
| GCTAGGGCAATGTGGGATTG+TGG | 0.564301 | 8.3:+65867681 | MS.gene59580:CDS |
| AAGAACAAGGAAATGAGAAG+AGG | 0.574484 | 8.3:+65867936 | MS.gene59580:CDS |
| ATGGCATTTCCATCTCTCCA+TGG | 0.584910 | 8.3:+65867765 | MS.gene59580:CDS |
| GATGAACAGGAAGCATTGAA+GGG | 0.594134 | 8.3:+65867630 | MS.gene59580:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTCATATGAATCATATAA+AGG | - | chr8.3:65867711-65867730 | None:intergenic | 20.0% |
| !! | GTTCATATGAATCATATAAA+GGG | - | chr8.3:65867710-65867729 | None:intergenic | 20.0% |
| !! | TTTATATGATTCATATGAAC+TGG | + | chr8.3:65867710-65867729 | MS.gene59580:CDS | 20.0% |
| ! | AGAAAAACTAAAGAAGAACA+AGG | + | chr8.3:65867923-65867942 | MS.gene59580:CDS | 25.0% |
| ! | TATTCATGATTCTCATGATA+TGG | + | chr8.3:65867812-65867831 | MS.gene59580:CDS | 25.0% |
| !! | GTAAATTTTCTTTCTTGTGA+AGG | + | chr8.3:65867975-65867994 | MS.gene59580:CDS | 25.0% |
| AGAAAGAAGAAGAAAAAGCT+AGG | + | chr8.3:65867664-65867683 | MS.gene59580:CDS | 30.0% | |
| AGAATTTGAAAAGGATGAAC+AGG | + | chr8.3:65867617-65867636 | MS.gene59580:CDS | 30.0% | |
| GAAAGAAGAAGAAAAAGCTA+GGG | + | chr8.3:65867665-65867684 | MS.gene59580:CDS | 30.0% | |
| GAGTGGAAAAGAATTTGAAA+AGG | + | chr8.3:65867608-65867627 | MS.gene59580:CDS | 30.0% | |
| ! | ACTCTTTTGCTTTCTCTATA+TGG | - | chr8.3:65867839-65867858 | None:intergenic | 30.0% |
| AAGAACAAGGAAATGAGAAG+AGG | + | chr8.3:65867936-65867955 | MS.gene59580:CDS | 35.0% | |
| AGAAAGCAAAAGAGTCTTCT+TGG | + | chr8.3:65867844-65867863 | MS.gene59580:CDS | 35.0% | |
| ATGATGAAGAGTAAGGATAG+TGG | + | chr8.3:65867897-65867916 | MS.gene59580:CDS | 35.0% | |
| GAAAGCAAAAGAGTCTTCTT+GGG | + | chr8.3:65867845-65867864 | MS.gene59580:CDS | 35.0% | |
| GATGAAGATGATGAAGAGTA+AGG | + | chr8.3:65867890-65867909 | MS.gene59580:CDS | 35.0% | |
| TCACATCAACAGGAATCTAA+TGG | + | chr8.3:65867746-65867765 | MS.gene59580:CDS | 35.0% | |
| AAGAAAAAGCTAGGGCAATG+TGG | + | chr8.3:65867673-65867692 | MS.gene59580:CDS | 40.0% | |
| AGAAAAAGCTAGGGCAATGT+GGG | + | chr8.3:65867674-65867693 | MS.gene59580:CDS | 40.0% | |
| CAAGGAAATGAGAAGAGGTA+TGG | + | chr8.3:65867941-65867960 | MS.gene59580:CDS | 40.0% | |
| CTCTTGATCATCACATCAAC+AGG | + | chr8.3:65867736-65867755 | MS.gene59580:CDS | 40.0% | |
| !! | CGATTATGCGTTTTGATCCA+TGG | - | chr8.3:65867785-65867804 | None:intergenic | 40.0% |
| !! | GATGAACAGGAAGCATTGAA+GGG | + | chr8.3:65867630-65867649 | MS.gene59580:CDS | 40.0% |
| ATGGCATTTCCATCTCTCCA+TGG | + | chr8.3:65867765-65867784 | MS.gene59580:CDS | 45.0% | |
| CTTCTTGGGTCACAAGCTTT+CGG | + | chr8.3:65867859-65867878 | MS.gene59580:CDS | 45.0% | |
| !! | CGTTTTGATCCATGGAGAGA+TGG | - | chr8.3:65867777-65867796 | None:intergenic | 45.0% |
| !! | GGATGAACAGGAAGCATTGA+AGG | + | chr8.3:65867629-65867648 | MS.gene59580:CDS | 45.0% |
| GCTAGGGCAATGTGGGATTG+TGG | + | chr8.3:65867681-65867700 | MS.gene59580:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 65867606 | 65868019 | 65867606 | ID=MS.gene59580 |
| chr8.3 | mRNA | 65867606 | 65868019 | 65867606 | ID=MS.gene59580.t1;Parent=MS.gene59580 |
| chr8.3 | exon | 65867606 | 65868019 | 65867606 | ID=MS.gene59580.t1.exon1;Parent=MS.gene59580.t1 |
| chr8.3 | CDS | 65867606 | 65868019 | 65867606 | ID=cds.MS.gene59580.t1;Parent=MS.gene59580.t1 |
| Gene Sequence |
| Protein sequence |
