Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052187.t1 | AES72186.1 | 91.8 | 122 | 9 | 1 | 1 | 122 | 1 | 121 | 5.50E-58 | 233.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052187.t1 | Q9LFT5 | 44.0 | 125 | 60 | 3 | 4 | 122 | 2 | 122 | 6.1e-21 | 101.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052187.t1 | G7J3Q1 | 91.8 | 122 | 9 | 1 | 1 | 122 | 1 | 121 | 4.0e-58 | 233.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049194 | MS.gene052187 | 0.811601 | 6.45E-51 | -1.69E-46 |
| MS.gene050100 | MS.gene052187 | 0.809871 | 1.53E-50 | -1.69E-46 |
| MS.gene05143 | MS.gene052187 | 0.801933 | 7.13E-49 | -1.69E-46 |
| MS.gene052142 | MS.gene052187 | 0.820801 | 5.66E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene053074 | 0.837961 | 3.81E-57 | -1.69E-46 |
| MS.gene052187 | MS.gene053383 | 0.804492 | 2.10E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene055359 | 0.868558 | 5.97E-66 | -1.69E-46 |
| MS.gene052187 | MS.gene058168 | 0.837463 | 5.12E-57 | -1.69E-46 |
| MS.gene052187 | MS.gene059006 | 0.821133 | 4.75E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene059632 | 0.849717 | 2.70E-60 | -1.69E-46 |
| MS.gene052187 | MS.gene061336 | 0.850976 | 1.20E-60 | -1.69E-46 |
| MS.gene052187 | MS.gene061337 | 0.834802 | 2.43E-56 | -1.69E-46 |
| MS.gene052187 | MS.gene072320 | 0.806032 | 1.00E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene07942 | 0.821261 | 4.44E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene08896 | 0.804576 | 2.02E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene22109 | 0.821088 | 4.86E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene25133 | 0.857236 | 1.87E-62 | -1.69E-46 |
| MS.gene052187 | MS.gene31254 | 0.834646 | 2.66E-56 | -1.69E-46 |
| MS.gene052187 | MS.gene31394 | 0.812769 | 3.59E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene32761 | 0.845571 | 3.74E-59 | -1.69E-46 |
| MS.gene052187 | MS.gene34106 | 0.821933 | 3.10E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene35428 | 0.813381 | 2.63E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene35695 | 0.817311 | 3.52E-52 | -1.69E-46 |
| MS.gene052187 | MS.gene36896 | 0.834444 | 2.99E-56 | -1.69E-46 |
| MS.gene052187 | MS.gene38528 | 0.808497 | 3.01E-50 | -1.69E-46 |
| MS.gene052187 | MS.gene38771 | 0.802096 | 6.60E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene39152 | 0.821809 | 3.31E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene42679 | 0.831126 | 1.99E-55 | -1.69E-46 |
| MS.gene052187 | MS.gene51424 | 0.812989 | 3.21E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene54033 | 0.817945 | 2.53E-52 | -1.69E-46 |
| MS.gene052187 | MS.gene54531 | 0.832551 | 8.86E-56 | -1.69E-46 |
| MS.gene052187 | MS.gene56024 | 0.818148 | 2.28E-52 | -1.69E-46 |
| MS.gene052187 | MS.gene56057 | 0.815152 | 1.07E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene56232 | 0.816475 | 5.43E-52 | -1.69E-46 |
| MS.gene052187 | MS.gene59580 | 0.804528 | 2.07E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene60245 | 0.801405 | 9.15E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene60986 | 0.826493 | 2.63E-54 | -1.69E-46 |
| MS.gene052187 | MS.gene61338 | 0.822909 | 1.84E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene62049 | 0.816059 | 6.72E-52 | -1.69E-46 |
| MS.gene052187 | MS.gene63972 | 0.805253 | 1.46E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene64425 | 0.811445 | 6.98E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene65481 | 0.805858 | 1.09E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene68655 | 0.83613 | 1.12E-56 | -1.69E-46 |
| MS.gene052187 | MS.gene68821 | 0.836042 | 1.18E-56 | -1.69E-46 |
| MS.gene052187 | MS.gene69587 | 0.811249 | 7.70E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene71187 | 0.814209 | 1.73E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene73229 | 0.80114 | 1.04E-48 | -1.69E-46 |
| MS.gene052187 | MS.gene74448 | 0.805995 | 1.02E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene75321 | 0.821757 | 3.41E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene75494 | 0.862956 | 3.51E-64 | -1.69E-46 |
| MS.gene052187 | MS.gene78446 | 0.801608 | 8.31E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene79672 | 0.822843 | 1.91E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene82810 | 0.846233 | 2.47E-59 | -1.69E-46 |
| MS.gene052187 | MS.gene83221 | 0.824202 | 9.17E-54 | -1.69E-46 |
| MS.gene052187 | MS.gene87013 | 0.80807 | 3.71E-50 | -1.69E-46 |
| MS.gene052187 | MS.gene88180 | 0.813364 | 2.66E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene90334 | 0.86711 | 1.74E-65 | -1.69E-46 |
| MS.gene052187 | MS.gene91762 | 0.826117 | 3.23E-54 | -1.69E-46 |
| MS.gene052187 | MS.gene91805 | 0.894315 | 2.77E-75 | -1.69E-46 |
| MS.gene052187 | MS.gene93760 | 0.850503 | 1.63E-60 | -1.69E-46 |
| MS.gene052187 | MS.gene94555 | 0.802005 | 6.89E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene96021 | 0.805949 | 1.04E-49 | -1.69E-46 |
| MS.gene052187 | MS.gene96503 | 0.814423 | 1.55E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052187.t1 | MTR_3g087020 | 91.803 | 122 | 9 | 1 | 1 | 122 | 1 | 121 | 3.37e-77 | 224 |
| MS.gene052187.t1 | MTR_8g090010 | 57.937 | 126 | 46 | 3 | 1 | 122 | 1 | 123 | 2.21e-35 | 118 |
| MS.gene052187.t1 | MTR_3g110550 | 41.860 | 86 | 38 | 2 | 40 | 122 | 54 | 130 | 4.66e-16 | 69.3 |
| MS.gene052187.t1 | MTR_4g097720 | 33.051 | 118 | 69 | 4 | 8 | 122 | 11 | 121 | 4.68e-14 | 63.9 |
| MS.gene052187.t1 | MTR_2g099810 | 42.105 | 76 | 27 | 2 | 47 | 122 | 70 | 128 | 1.14e-13 | 63.2 |
| MS.gene052187.t1 | MTR_4g114660 | 38.824 | 85 | 38 | 2 | 39 | 122 | 43 | 114 | 4.62e-13 | 61.2 |
| MS.gene052187.t1 | MTR_2g103780 | 34.146 | 123 | 69 | 5 | 1 | 122 | 2 | 113 | 1.77e-12 | 59.7 |
| MS.gene052187.t1 | MTR_5g014840 | 37.662 | 77 | 43 | 2 | 46 | 122 | 60 | 131 | 5.18e-12 | 58.9 |
| MS.gene052187.t1 | MTR_8g017850 | 37.975 | 79 | 28 | 2 | 44 | 122 | 72 | 129 | 4.76e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052187.t1 | AT5G10310 | 48.544 | 103 | 43 | 3 | 26 | 122 | 24 | 122 | 4.15e-26 | 94.7 |
| MS.gene052187.t1 | AT5G10310 | 45.872 | 109 | 43 | 4 | 26 | 122 | 24 | 128 | 1.16e-23 | 89.0 |
Find 33 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAACCTCATCTTGTTAA+TGG | 0.240049 | 3.2:+74307915 | MS.gene052187:CDS |
| ATCCGTTATTATTATTACCT+TGG | 0.417680 | 3.2:-74308472 | None:intergenic |
| GGTTGATAGAAGCAAGAAAC+TGG | 0.429204 | 3.2:-74307970 | None:intergenic |
| GGCTGAACATGTTCATGGCT+TGG | 0.438174 | 3.2:-74308401 | None:intergenic |
| GACCTGGCTGAACATGTTCA+TGG | 0.454543 | 3.2:-74308406 | None:intergenic |
| CTCCAAGGTAATAATAATAA+CGG | 0.464837 | 3.2:+74308470 | MS.gene052187:CDS |
| TGTGTAGGGTTTATTATTTG+AGG | 0.467998 | 3.2:+74308298 | MS.gene052187:intron |
| AAGAAACTGGACACAGTAGA+AGG | 0.491502 | 3.2:-74307957 | None:intergenic |
| CAATGGCATTTCCAACTCAA+TGG | 0.495776 | 3.2:-74308509 | None:intergenic |
| AAAGAAATTACCACCTTCCA+TGG | 0.501489 | 3.2:-74308447 | None:intergenic |
| GGAACTTGCACTGCCATGCA+TGG | 0.505648 | 3.2:-74308380 | None:intergenic |
| CACTGCCTCAACTTCATCCA+TGG | 0.509432 | 3.2:+74308430 | MS.gene052187:CDS |
| CATTTGTTGTGACATGTTGG+TGG | 0.511699 | 3.2:-74308344 | None:intergenic |
| AGGGAAAAGCCTACCCTTGG+AGG | 0.512819 | 3.2:-74308006 | None:intergenic |
| ATTTCTAAGGGTTGAAGAAG+TGG | 0.515036 | 3.2:-74308538 | None:intergenic |
| CAAATTACAAGCCATTGAGT+TGG | 0.518087 | 3.2:+74308498 | MS.gene052187:CDS |
| GGTGGTAATTTCTTTCTCCA+AGG | 0.542564 | 3.2:+74308455 | MS.gene052187:CDS |
| CACAATCTGCAATTCCTCCA+AGG | 0.549029 | 3.2:+74307992 | MS.gene052187:CDS |
| AGCCATGAACATGTTCAGCC+AGG | 0.557195 | 3.2:+74308404 | MS.gene052187:CDS |
| GAAGTTGAGGCAGTGTGACC+TGG | 0.560053 | 3.2:-74308422 | None:intergenic |
| ACCTTCCATGGATGAAGTTG+AGG | 0.576895 | 3.2:-74308435 | None:intergenic |
| GCCTCAACTTCATCCATGGA+AGG | 0.583985 | 3.2:+74308434 | MS.gene052187:CDS |
| TTACATTTGTTGTGACATGT+TGG | 0.595097 | 3.2:-74308347 | None:intergenic |
| CTTGGAGGAATTGCAGATTG+TGG | 0.597288 | 3.2:-74307991 | None:intergenic |
| ATATGAATTAAATGAAGCCA+TGG | 0.598193 | 3.2:-74307896 | None:intergenic |
| TGATGACCATTAACAAGATG+AGG | 0.607087 | 3.2:-74307921 | None:intergenic |
| TTGAGTTGGAAATGCCATTG+TGG | 0.619909 | 3.2:+74308512 | MS.gene052187:CDS |
| TGAAGAAGTGGCCACCACAA+TGG | 0.621299 | 3.2:-74308526 | None:intergenic |
| TAACCAATGTCATCCATGCA+TGG | 0.626151 | 3.2:+74308367 | MS.gene052187:CDS |
| ACAATCTGCAATTCCTCCAA+GGG | 0.630675 | 3.2:+74307993 | MS.gene052187:CDS |
| TCAACTTCATCCATGGAAGG+TGG | 0.660618 | 3.2:+74308437 | MS.gene052187:CDS |
| CTGCCATGCATGGATGACAT+TGG | 0.662535 | 3.2:-74308370 | None:intergenic |
| AGTTGGAAATGCCATTGTGG+TGG | 0.687706 | 3.2:+74308515 | MS.gene052187:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTAACAAACTTAATTAAC+TGG | - | chr3.2:74308275-74308294 | None:intergenic | 20.0% |
| !! | GTTTGAATTCACTATAAAAT+TGG | - | chr3.2:74308200-74308219 | None:intergenic | 20.0% |
| ! | AATTCACTATAAAATTGGCT+CGG | - | chr3.2:74308195-74308214 | None:intergenic | 25.0% |
| ! | ATATAACCTCATCTTGTTAA+TGG | + | chr3.2:74307915-74307934 | MS.gene052187:CDS | 25.0% |
| ! | ATCCGTTATTATTATTACCT+TGG | - | chr3.2:74308475-74308494 | None:intergenic | 25.0% |
| ! | CTCCAAGGTAATAATAATAA+CGG | + | chr3.2:74308470-74308489 | MS.gene052187:CDS | 25.0% |
| ! | TATTATTTGAGGAGAAAAAC+AGG | + | chr3.2:74308309-74308328 | MS.gene052187:CDS | 25.0% |
| !! | ACAGTATATTACTGTGATTT+TGG | + | chr3.2:74308134-74308153 | MS.gene052187:intron | 25.0% |
| !!! | TAGTTGGTTTTAAAAGGAAT+CGG | + | chr3.2:74308076-74308095 | MS.gene052187:intron | 25.0% |
| ACAGTAATATACTGTGATTC+TGG | - | chr3.2:74308129-74308148 | None:intergenic | 30.0% | |
| ACTTAATTAACTGGTGAGTT+TGG | - | chr3.2:74308266-74308285 | None:intergenic | 30.0% | |
| TGTGTAGGGTTTATTATTTG+AGG | + | chr3.2:74308298-74308317 | MS.gene052187:intron | 30.0% | |
| TTATGTGTATGTTGCATAGT+TGG | + | chr3.2:74308060-74308079 | MS.gene052187:intron | 30.0% | |
| ! | ATCCTCATTTTGTTGAAACT+TGG | - | chr3.2:74308228-74308247 | None:intergenic | 30.0% |
| ! | GTTTGTTAACTATGTGTGTA+GGG | + | chr3.2:74308284-74308303 | MS.gene052187:intron | 30.0% |
| !! | AGTTTGTTAACTATGTGTGT+AGG | + | chr3.2:74308283-74308302 | MS.gene052187:intron | 30.0% |
| !! | TTACATTTGTTGTGACATGT+TGG | - | chr3.2:74308350-74308369 | None:intergenic | 30.0% |
| !!! | GTTGCATAGTTGGTTTTAAA+AGG | + | chr3.2:74308070-74308089 | MS.gene052187:intron | 30.0% |
| AAAGAAATTACCACCTTCCA+TGG | - | chr3.2:74308450-74308469 | None:intergenic | 35.0% | |
| AGTGAATGAGGGAGAAAAAA+GGG | - | chr3.2:74308028-74308047 | None:intergenic | 35.0% | |
| CAAATTACAAGCCATTGAGT+TGG | + | chr3.2:74308498-74308517 | MS.gene052187:CDS | 35.0% | |
| CACCAAGTTTCAACAAAATG+AGG | + | chr3.2:74308223-74308242 | MS.gene052187:intron | 35.0% | |
| GCAACATACACATAAAGCAA+AGG | - | chr3.2:74308055-74308074 | None:intergenic | 35.0% | |
| TGATGACCATTAACAAGATG+AGG | - | chr3.2:74307924-74307943 | None:intergenic | 35.0% | |
| TTTGAGGAGAAAAACAGGTT+AGG | + | chr3.2:74308314-74308333 | MS.gene052187:CDS | 35.0% | |
| AAGAAACTGGACACAGTAGA+AGG | - | chr3.2:74307960-74307979 | None:intergenic | 40.0% | |
| ACAATCTGCAATTCCTCCAA+GGG | + | chr3.2:74307993-74308012 | MS.gene052187:CDS | 40.0% | |
| AGCAAAGGAAAGAGTGAATG+AGG | - | chr3.2:74308040-74308059 | None:intergenic | 40.0% | |
| CAATGGCATTTCCAACTCAA+TGG | - | chr3.2:74308512-74308531 | None:intergenic | 40.0% | |
| GAGTGAATGAGGGAGAAAAA+AGG | - | chr3.2:74308029-74308048 | None:intergenic | 40.0% | |
| GCAAAGGAAAGAGTGAATGA+GGG | - | chr3.2:74308039-74308058 | None:intergenic | 40.0% | |
| GGTGGTAATTTCTTTCTCCA+AGG | + | chr3.2:74308455-74308474 | MS.gene052187:CDS | 40.0% | |
| TAACCAATGTCATCCATGCA+TGG | + | chr3.2:74308367-74308386 | MS.gene052187:CDS | 40.0% | |
| TGGTGAGTTTGGCAAAATCA+AGG | - | chr3.2:74308255-74308274 | None:intergenic | 40.0% | |
| !! | CATTTGTTGTGACATGTTGG+TGG | - | chr3.2:74308347-74308366 | None:intergenic | 40.0% |
| !! | GGTTGATAGAAGCAAGAAAC+TGG | - | chr3.2:74307973-74307992 | None:intergenic | 40.0% |
| !! | TTGAGTTGGAAATGCCATTG+TGG | + | chr3.2:74308512-74308531 | MS.gene052187:CDS | 40.0% |
| AAAAGGGAAAAGCCTACCCT+TGG | - | chr3.2:74308012-74308031 | None:intergenic | 45.0% | |
| ACCTTCCATGGATGAAGTTG+AGG | - | chr3.2:74308438-74308457 | None:intergenic | 45.0% | |
| AGTTGGAAATGCCATTGTGG+TGG | + | chr3.2:74308515-74308534 | MS.gene052187:CDS | 45.0% | |
| CACAATCTGCAATTCCTCCA+AGG | + | chr3.2:74307992-74308011 | MS.gene052187:CDS | 45.0% | |
| CTTGGAGGAATTGCAGATTG+TGG | - | chr3.2:74307994-74308013 | None:intergenic | 45.0% | |
| GCTCGGAGCTTCAACAAAAT+CGG | - | chr3.2:74308178-74308197 | None:intergenic | 45.0% | |
| GGAATCGGTGCATGTTTGAA+TGG | + | chr3.2:74308091-74308110 | MS.gene052187:intron | 45.0% | |
| TCAACTTCATCCATGGAAGG+TGG | + | chr3.2:74308437-74308456 | MS.gene052187:CDS | 45.0% | |
| TCGGTGCATGTTTGAATGGA+CGG | + | chr3.2:74308095-74308114 | MS.gene052187:intron | 45.0% | |
| AGCCATGAACATGTTCAGCC+AGG | + | chr3.2:74308404-74308423 | MS.gene052187:CDS | 50.0% | |
| CACTGCCTCAACTTCATCCA+TGG | + | chr3.2:74308430-74308449 | MS.gene052187:CDS | 50.0% | |
| CTGCCATGCATGGATGACAT+TGG | - | chr3.2:74308373-74308392 | None:intergenic | 50.0% | |
| GACCTGGCTGAACATGTTCA+TGG | - | chr3.2:74308409-74308428 | None:intergenic | 50.0% | |
| GCCTCAACTTCATCCATGGA+AGG | + | chr3.2:74308434-74308453 | MS.gene052187:CDS | 50.0% | |
| GGCTGAACATGTTCATGGCT+TGG | - | chr3.2:74308404-74308423 | None:intergenic | 50.0% | |
| TCTGCAATTCCTCCAAGGGT+AGG | + | chr3.2:74307997-74308016 | MS.gene052187:intron | 50.0% | |
| TGAAGAAGTGGCCACCACAA+TGG | - | chr3.2:74308529-74308548 | None:intergenic | 50.0% | |
| AGGGAAAAGCCTACCCTTGG+AGG | - | chr3.2:74308009-74308028 | None:intergenic | 55.0% | |
| GAAGTTGAGGCAGTGTGACC+TGG | - | chr3.2:74308425-74308444 | None:intergenic | 55.0% | |
| GGAACTTGCACTGCCATGCA+TGG | - | chr3.2:74308383-74308402 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 74307898 | 74308556 | 74307898 | ID=MS.gene052187 |
| chr3.2 | mRNA | 74307898 | 74308556 | 74307898 | ID=MS.gene052187.t1;Parent=MS.gene052187 |
| chr3.2 | exon | 74307898 | 74308014 | 74307898 | ID=MS.gene052187.t1.exon1;Parent=MS.gene052187.t1 |
| chr3.2 | CDS | 74307898 | 74308014 | 74307898 | ID=cds.MS.gene052187.t1;Parent=MS.gene052187.t1 |
| chr3.2 | exon | 74308305 | 74308556 | 74308305 | ID=MS.gene052187.t1.exon2;Parent=MS.gene052187.t1 |
| chr3.2 | CDS | 74308305 | 74308556 | 74308305 | ID=cds.MS.gene052187.t1;Parent=MS.gene052187.t1 |
| Gene Sequence |
| Protein sequence |
