Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59597.t1 | RHN39778.1 | 87.9 | 199 | 22 | 1 | 12 | 208 | 314 | 512 | 1.70E-91 | 345.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59597.t1 | P54771 | 54.1 | 196 | 86 | 2 | 12 | 205 | 323 | 516 | 1.6e-53 | 210.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59597.t1 | A0A072TPD4 | 87.9 | 199 | 22 | 1 | 12 | 208 | 304 | 502 | 1.2e-91 | 345.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057750 | MS.gene59597 | 0.809759 | 1.61E-50 | -1.69E-46 |
| MS.gene057754 | MS.gene59597 | 0.813508 | 2.47E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59597.t1 | MTR_8g028060 | 87.940 | 199 | 22 | 1 | 12 | 208 | 304 | 502 | 1.45e-123 | 358 |
| MS.gene59597.t1 | MTR_2g029430 | 55.869 | 213 | 73 | 4 | 12 | 203 | 303 | 515 | 7.66e-74 | 232 |
| MS.gene59597.t1 | MTR_2g029450 | 55.869 | 213 | 73 | 4 | 12 | 203 | 303 | 515 | 7.66e-74 | 232 |
| MS.gene59597.t1 | MTR_7g058700 | 60.204 | 196 | 74 | 3 | 12 | 203 | 303 | 498 | 2.78e-72 | 235 |
| MS.gene59597.t1 | MTR_7g098730 | 45.876 | 194 | 87 | 2 | 12 | 203 | 326 | 503 | 2.61e-57 | 189 |
| MS.gene59597.t1 | MTR_7g098700 | 45.876 | 194 | 87 | 2 | 12 | 203 | 352 | 529 | 8.48e-56 | 185 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59597.t1 | AT2G20340 | 51.546 | 194 | 76 | 2 | 12 | 203 | 311 | 488 | 8.44e-65 | 207 |
| MS.gene59597.t1 | AT2G20340 | 52.778 | 180 | 69 | 1 | 24 | 203 | 298 | 461 | 2.96e-63 | 203 |
| MS.gene59597.t1 | AT4G28680 | 48.588 | 177 | 75 | 2 | 24 | 200 | 377 | 537 | 3.80e-53 | 178 |
| MS.gene59597.t1 | AT4G28680 | 48.588 | 177 | 75 | 2 | 24 | 200 | 370 | 530 | 4.08e-53 | 178 |
| MS.gene59597.t1 | AT4G28680 | 48.588 | 177 | 75 | 2 | 24 | 200 | 379 | 539 | 4.23e-53 | 178 |
| MS.gene59597.t1 | AT4G28680 | 48.045 | 179 | 75 | 3 | 24 | 200 | 377 | 539 | 1.37e-51 | 174 |
| MS.gene59597.t1 | AT4G28680 | 41.951 | 205 | 75 | 3 | 24 | 200 | 370 | 558 | 2.31e-47 | 163 |
Find 32 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGAAATCATGTGAAAA+TGG | 0.213136 | 8.2:-69243654 | MS.gene59597:CDS |
| TTCTTGTTCCTCAAGAATTC+AGG | 0.263166 | 8.2:+69243797 | None:intergenic |
| AATTCAATGCACTCAAACTA+TGG | 0.272569 | 8.2:-69243718 | MS.gene59597:CDS |
| AATGATGAATGATGCATATT+TGG | 0.273967 | 8.2:-69243492 | MS.gene59597:CDS |
| CTAGATTCAATTAATAGTTC+TGG | 0.298216 | 8.2:-69243446 | MS.gene59597:CDS |
| GCATTGAATTTCCTACTTAG+AGG | 0.299375 | 8.2:+69243731 | None:intergenic |
| GAATTTAGACATTACATTGA+TGG | 0.314406 | 8.2:-69243938 | MS.gene59597:CDS |
| TGTGCCAAGTAAATTCTCTT+TGG | 0.351339 | 8.2:-69243582 | MS.gene59597:CDS |
| CACATGATGTTCTTCTGTTA+AGG | 0.374410 | 8.2:+69243358 | None:intergenic |
| TGTGACTATGACATGGAAAT+TGG | 0.375252 | 8.2:-69243339 | MS.gene59597:CDS |
| GGTAGTGAAGGTTACTTTGT+TGG | 0.379028 | 8.2:-69243518 | MS.gene59597:CDS |
| TCAATGTAATGTCTAAATTC+AGG | 0.399622 | 8.2:+69243941 | None:intergenic |
| TTTGTTATTAGATGTGCTAT+TGG | 0.414656 | 8.2:-69243386 | MS.gene59597:CDS |
| TTAGCAATACTAACTGCTAA+TGG | 0.417365 | 8.2:+69243542 | None:intergenic |
| CAAACCAAAGAGAATTTACT+TGG | 0.423556 | 8.2:+69243578 | None:intergenic |
| TTAGCAGTTAGTATTGCTAA+TGG | 0.439971 | 8.2:-69243539 | MS.gene59597:CDS |
| AAGTTATTGACTACAAAGAT+TGG | 0.481411 | 8.2:-69243760 | MS.gene59597:CDS |
| ATGAGTCATGGTGAGGTTGA+AGG | 0.500084 | 8.2:-69243413 | MS.gene59597:CDS |
| CATGTCGATGCTGCTTATGC+CGG | 0.503291 | 8.2:-69243980 | MS.gene59597:CDS |
| TTCAATTAATAGTTCTGGCA+AGG | 0.519470 | 8.2:-69243441 | MS.gene59597:CDS |
| ATTGGCAAATACCTCTAAGT+AGG | 0.523602 | 8.2:-69243742 | MS.gene59597:CDS |
| CAACAAATCCTGAATTCTTG+AGG | 0.537102 | 8.2:-69243805 | MS.gene59597:CDS |
| TGGATTGTTCTTCGAAGCTA+CGG | 0.551140 | 8.2:-69243698 | MS.gene59597:CDS |
| GACTAGTGAAGAAAGATGAG+AGG | 0.555451 | 8.2:-69243616 | MS.gene59597:CDS |
| AGTATTGCTAATGGTAGTGA+AGG | 0.560220 | 8.2:-69243530 | MS.gene59597:CDS |
| AATTCAGGACAAATACAAGC+TGG | 0.567486 | 8.2:+69243956 | None:intergenic |
| TATATTATACATTAGTTGAG+AGG | 0.568718 | 8.2:+69243284 | None:intergenic |
| AGGACAAATACAAGCTGGAC+CGG | 0.569058 | 8.2:+69243961 | None:intergenic |
| TTACATTGATGGAATTGAAG+AGG | 0.594140 | 8.2:-69243927 | MS.gene59597:intron |
| GGCAAGGCGTACATGAGTCA+TGG | 0.605690 | 8.2:-69243425 | MS.gene59597:CDS |
| AACATCATGTGACTATGACA+TGG | 0.637027 | 8.2:-69243346 | MS.gene59597:CDS |
| GGCGTACATGAGTCATGGTG+AGG | 0.665486 | 8.2:-69243420 | MS.gene59597:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATGGCAAAAATATTTGA+AGG | - | chr8.2:69243636-69243655 | MS.gene59597:CDS | 20.0% |
| ! | AATGATGAATGATGCATATT+TGG | - | chr8.2:69243782-69243801 | MS.gene59597:CDS | 25.0% |
| ! | CTAGATTCAATTAATAGTTC+TGG | - | chr8.2:69243828-69243847 | MS.gene59597:CDS | 25.0% |
| ! | GAATTTAGACATTACATTGA+TGG | - | chr8.2:69243336-69243355 | MS.gene59597:CDS | 25.0% |
| ! | TCAATGTAATGTCTAAATTC+AGG | + | chr8.2:69243336-69243355 | None:intergenic | 25.0% |
| ! | TTTGAGAAATCATGTGAAAA+TGG | - | chr8.2:69243620-69243639 | MS.gene59597:CDS | 25.0% |
| !! | AAGTTATTGACTACAAAGAT+TGG | - | chr8.2:69243514-69243533 | MS.gene59597:CDS | 25.0% |
| !!! | TTAGCTTTAATGCACATAAA+TGG | - | chr8.2:69243379-69243398 | MS.gene59597:CDS | 25.0% |
| !!! | TTTGTTATTAGATGTGCTAT+TGG | - | chr8.2:69243888-69243907 | MS.gene59597:intron | 25.0% |
| AATTCAATGCACTCAAACTA+TGG | - | chr8.2:69243556-69243575 | MS.gene59597:CDS | 30.0% | |
| ATTTAGCATGTTGTTGTCTT+TGG | - | chr8.2:69243412-69243431 | MS.gene59597:CDS | 30.0% | |
| CAAACCAAAGAGAATTTACT+TGG | + | chr8.2:69243699-69243718 | None:intergenic | 30.0% | |
| TTACATTGATGGAATTGAAG+AGG | - | chr8.2:69243347-69243366 | MS.gene59597:CDS | 30.0% | |
| TTAGCAATACTAACTGCTAA+TGG | + | chr8.2:69243735-69243754 | None:intergenic | 30.0% | |
| TTAGCAGTTAGTATTGCTAA+TGG | - | chr8.2:69243735-69243754 | MS.gene59597:CDS | 30.0% | |
| TTTAGCATGTTGTTGTCTTT+GGG | - | chr8.2:69243413-69243432 | MS.gene59597:CDS | 30.0% | |
| ! | CAACATGCAAGTTTTCTATT+AGG | - | chr8.2:69243963-69243982 | MS.gene59597:CDS | 30.0% |
| !! | TTCAATTAATAGTTCTGGCA+AGG | - | chr8.2:69243833-69243852 | MS.gene59597:intron | 30.0% |
| AACATCATGTGACTATGACA+TGG | - | chr8.2:69243928-69243947 | MS.gene59597:CDS | 35.0% | |
| AATTCAGGACAAATACAAGC+TGG | + | chr8.2:69243321-69243340 | None:intergenic | 35.0% | |
| ATTGGCAAATACCTCTAAGT+AGG | - | chr8.2:69243532-69243551 | MS.gene59597:CDS | 35.0% | |
| CAACAAATCCTGAATTCTTG+AGG | - | chr8.2:69243469-69243488 | MS.gene59597:CDS | 35.0% | |
| CACATGATGTTCTTCTGTTA+AGG | + | chr8.2:69243919-69243938 | None:intergenic | 35.0% | |
| GCATTGAATTTCCTACTTAG+AGG | + | chr8.2:69243546-69243565 | None:intergenic | 35.0% | |
| TGTGACTATGACATGGAAAT+TGG | - | chr8.2:69243935-69243954 | MS.gene59597:CDS | 35.0% | |
| TGTGCCAAGTAAATTCTCTT+TGG | - | chr8.2:69243692-69243711 | MS.gene59597:CDS | 35.0% | |
| TTCTTGTTCCTCAAGAATTC+AGG | + | chr8.2:69243480-69243499 | None:intergenic | 35.0% | |
| ! | AGTATTGCTAATGGTAGTGA+AGG | - | chr8.2:69243744-69243763 | MS.gene59597:CDS | 35.0% |
| GACTAGTGAAGAAAGATGAG+AGG | - | chr8.2:69243658-69243677 | MS.gene59597:CDS | 40.0% | |
| TGGATTGTTCTTCGAAGCTA+CGG | - | chr8.2:69243576-69243595 | MS.gene59597:CDS | 40.0% | |
| ! | GGTAGTGAAGGTTACTTTGT+TGG | - | chr8.2:69243756-69243775 | MS.gene59597:CDS | 40.0% |
| AGGACAAATACAAGCTGGAC+CGG | + | chr8.2:69243316-69243335 | None:intergenic | 45.0% | |
| ATGAGTCATGGTGAGGTTGA+AGG | - | chr8.2:69243861-69243880 | MS.gene59597:intron | 45.0% | |
| GGCAAGGCGTACATGAGTCA+TGG | - | chr8.2:69243849-69243868 | MS.gene59597:intron | 55.0% | |
| GGCGTACATGAGTCATGGTG+AGG | - | chr8.2:69243854-69243873 | MS.gene59597:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 69243295 | 69244001 | 69243295 | ID=MS.gene59597 |
| chr8.2 | mRNA | 69243295 | 69244001 | 69243295 | ID=MS.gene59597.t1;Parent=MS.gene59597 |
| chr8.2 | exon | 69243928 | 69244001 | 69243928 | ID=MS.gene59597.t1.exon1;Parent=MS.gene59597.t1 |
| chr8.2 | CDS | 69243928 | 69244001 | 69243928 | ID=cds.MS.gene59597.t1;Parent=MS.gene59597.t1 |
| chr8.2 | exon | 69243295 | 69243847 | 69243295 | ID=MS.gene59597.t1.exon2;Parent=MS.gene59597.t1 |
| chr8.2 | CDS | 69243295 | 69243847 | 69243295 | ID=cds.MS.gene59597.t1;Parent=MS.gene59597.t1 |
| Gene Sequence |
| Protein sequence |
