Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60312.t1 | XP_013457856.1 | 96.3 | 515 | 15 | 1 | 1 | 511 | 6 | 520 | 1.40E-280 | 974.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60312.t1 | Q9FLD5 | 57.4 | 467 | 186 | 4 | 1 | 461 | 4 | 463 | 2.8e-152 | 540.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60312.t1 | A0A072UQF5 | 96.3 | 515 | 15 | 1 | 1 | 511 | 6 | 520 | 1.0e-280 | 974.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055251 | MS.gene60312 | 0.819714 | 1.00E-52 | -1.69E-46 |
| MS.gene056266 | MS.gene60312 | -0.837644 | 4.60E-57 | -1.69E-46 |
| MS.gene060359 | MS.gene60312 | 0.811679 | 6.20E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60312.t1 | MTR_4g107370 | 97.864 | 515 | 7 | 1 | 1 | 511 | 6 | 520 | 0.0 | 1045 |
| MS.gene60312.t1 | MTR_1g022250 | 62.420 | 471 | 162 | 3 | 3 | 472 | 5 | 461 | 0.0 | 603 |
| MS.gene60312.t1 | MTR_7g008040 | 62.121 | 462 | 171 | 3 | 1 | 461 | 1 | 459 | 0.0 | 597 |
| MS.gene60312.t1 | MTR_2g030420 | 61.441 | 472 | 162 | 3 | 2 | 472 | 7 | 459 | 0.0 | 591 |
| MS.gene60312.t1 | MTR_6g009540 | 60.173 | 462 | 173 | 5 | 3 | 461 | 5 | 458 | 0.0 | 563 |
| MS.gene60312.t1 | MTR_6g009520 | 59.524 | 462 | 176 | 4 | 3 | 461 | 7 | 460 | 0.0 | 561 |
| MS.gene60312.t1 | MTR_6g009720 | 57.479 | 468 | 184 | 5 | 3 | 461 | 7 | 468 | 0.0 | 537 |
| MS.gene60312.t1 | MTR_1g022245 | 54.860 | 463 | 204 | 3 | 1 | 461 | 1 | 460 | 0.0 | 528 |
| MS.gene60312.t1 | MTR_6g009553 | 58.000 | 450 | 171 | 6 | 24 | 461 | 4 | 447 | 0.0 | 516 |
| MS.gene60312.t1 | MTR_6g009770 | 55.011 | 469 | 172 | 8 | 3 | 461 | 7 | 446 | 2.97e-171 | 493 |
| MS.gene60312.t1 | MTR_6g042290 | 53.996 | 463 | 175 | 7 | 8 | 463 | 21 | 452 | 2.06e-168 | 486 |
| MS.gene60312.t1 | MTR_6g009510 | 49.891 | 459 | 155 | 7 | 8 | 463 | 21 | 407 | 6.54e-147 | 429 |
| MS.gene60312.t1 | MTR_6g009660 | 46.695 | 469 | 176 | 10 | 4 | 461 | 5 | 410 | 2.44e-130 | 387 |
| MS.gene60312.t1 | MTR_4g128260 | 41.752 | 491 | 261 | 10 | 1 | 480 | 1 | 477 | 3.59e-123 | 370 |
| MS.gene60312.t1 | MTR_2g076970 | 42.060 | 466 | 223 | 9 | 7 | 465 | 6 | 431 | 3.81e-121 | 363 |
| MS.gene60312.t1 | MTR_2g076920 | 41.200 | 500 | 234 | 11 | 10 | 495 | 15 | 468 | 3.79e-120 | 361 |
| MS.gene60312.t1 | MTR_2g076690 | 41.042 | 480 | 234 | 7 | 10 | 480 | 16 | 455 | 2.39e-117 | 353 |
| MS.gene60312.t1 | MTR_2g076710 | 41.164 | 464 | 229 | 9 | 10 | 465 | 14 | 441 | 3.89e-117 | 353 |
| MS.gene60312.t1 | MTR_2g076840 | 42.038 | 471 | 224 | 9 | 8 | 470 | 7 | 436 | 4.49e-116 | 359 |
| MS.gene60312.t1 | MTR_2g076840 | 51.136 | 264 | 101 | 2 | 198 | 461 | 467 | 702 | 1.39e-84 | 276 |
| MS.gene60312.t1 | MTR_5g020980 | 37.773 | 503 | 268 | 9 | 2 | 493 | 11 | 479 | 1.29e-114 | 348 |
| MS.gene60312.t1 | MTR_2g076900 | 40.486 | 494 | 238 | 12 | 10 | 495 | 9 | 454 | 4.56e-114 | 345 |
| MS.gene60312.t1 | MTR_2g061820 | 39.959 | 493 | 260 | 11 | 1 | 487 | 4 | 466 | 1.16e-111 | 341 |
| MS.gene60312.t1 | MTR_2g076990 | 43.256 | 430 | 198 | 9 | 61 | 481 | 1 | 393 | 3.52e-110 | 335 |
| MS.gene60312.t1 | MTR_8g076920 | 36.614 | 508 | 277 | 8 | 4 | 496 | 23 | 500 | 1.23e-105 | 325 |
| MS.gene60312.t1 | MTR_5g020990 | 34.375 | 480 | 265 | 10 | 5 | 465 | 25 | 473 | 8.96e-89 | 281 |
| MS.gene60312.t1 | MTR_3g103150 | 42.903 | 310 | 135 | 5 | 156 | 461 | 4 | 275 | 3.08e-79 | 249 |
| MS.gene60312.t1 | MTR_3g092060 | 29.945 | 541 | 266 | 16 | 4 | 491 | 2 | 482 | 2.56e-66 | 222 |
| MS.gene60312.t1 | MTR_7g117950 | 31.765 | 425 | 224 | 10 | 41 | 461 | 42 | 404 | 4.31e-65 | 218 |
| MS.gene60312.t1 | MTR_5g021020 | 36.111 | 324 | 151 | 9 | 140 | 461 | 135 | 404 | 9.31e-53 | 184 |
| MS.gene60312.t1 | MTR_8g027200 | 28.817 | 465 | 237 | 13 | 30 | 475 | 29 | 418 | 6.04e-51 | 180 |
| MS.gene60312.t1 | MTR_1g011680 | 51.807 | 166 | 78 | 1 | 333 | 496 | 3 | 168 | 5.70e-45 | 156 |
| MS.gene60312.t1 | MTR_5g021000 | 47.200 | 125 | 61 | 2 | 357 | 479 | 1 | 122 | 5.70e-31 | 118 |
| MS.gene60312.t1 | MTR_5g020720 | 39.888 | 178 | 78 | 6 | 263 | 438 | 26 | 176 | 7.41e-27 | 107 |
| MS.gene60312.t1 | MTR_1g113750 | 47.368 | 95 | 50 | 0 | 390 | 484 | 16 | 110 | 2.51e-20 | 87.4 |
| MS.gene60312.t1 | MTR_5g021010 | 46.667 | 90 | 43 | 2 | 374 | 461 | 34 | 120 | 5.60e-18 | 80.5 |
| MS.gene60312.t1 | MTR_2g076820 | 52.174 | 69 | 30 | 1 | 390 | 458 | 1 | 66 | 8.99e-16 | 72.8 |
| MS.gene60312.t1 | MTR_7g010800 | 28.372 | 215 | 122 | 8 | 202 | 410 | 206 | 394 | 1.24e-12 | 70.9 |
| MS.gene60312.t1 | MTR_4g094662 | 28.505 | 214 | 121 | 8 | 203 | 410 | 230 | 417 | 1.72e-12 | 70.5 |
| MS.gene60312.t1 | MTR_2g438140 | 28.037 | 214 | 122 | 8 | 203 | 410 | 227 | 414 | 3.97e-12 | 69.3 |
| MS.gene60312.t1 | MTR_2g438140 | 28.037 | 214 | 122 | 8 | 203 | 410 | 287 | 474 | 6.69e-12 | 68.6 |
| MS.gene60312.t1 | MTR_2g101290 | 29.630 | 189 | 104 | 5 | 218 | 403 | 430 | 592 | 1.48e-11 | 67.4 |
| MS.gene60312.t1 | MTR_2g101290 | 29.630 | 189 | 104 | 5 | 218 | 403 | 430 | 592 | 2.09e-11 | 67.0 |
| MS.gene60312.t1 | MTR_4g094270 | 27.919 | 197 | 114 | 6 | 202 | 395 | 156 | 327 | 5.33e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60312.t1 | AT5G40010 | 57.759 | 464 | 183 | 4 | 1 | 458 | 4 | 460 | 0.0 | 562 |
| MS.gene60312.t1 | AT3G28580 | 57.634 | 465 | 177 | 7 | 1 | 458 | 4 | 455 | 0.0 | 530 |
| MS.gene60312.t1 | AT3G28510 | 54.212 | 463 | 203 | 3 | 4 | 465 | 7 | 461 | 3.39e-176 | 506 |
| MS.gene60312.t1 | AT3G28540 | 54.746 | 453 | 195 | 3 | 9 | 460 | 12 | 455 | 1.37e-173 | 499 |
| MS.gene60312.t1 | AT3G28540 | 54.746 | 453 | 195 | 3 | 9 | 460 | 12 | 455 | 1.45e-173 | 499 |
| MS.gene60312.t1 | AT3G28580 | 54.095 | 464 | 173 | 7 | 1 | 458 | 4 | 433 | 1.86e-169 | 487 |
| MS.gene60312.t1 | AT3G28600 | 52.101 | 476 | 185 | 7 | 4 | 465 | 3 | 449 | 4.82e-166 | 479 |
| MS.gene60312.t1 | AT3G28610 | 49.507 | 507 | 197 | 9 | 4 | 491 | 2 | 468 | 1.48e-164 | 474 |
| MS.gene60312.t1 | AT5G40000 | 48.381 | 494 | 201 | 11 | 1 | 473 | 4 | 464 | 1.04e-154 | 449 |
| MS.gene60312.t1 | AT3G28520 | 50.881 | 454 | 206 | 4 | 12 | 461 | 1 | 441 | 1.30e-152 | 444 |
| MS.gene60312.t1 | AT3G28600 | 48.739 | 476 | 162 | 6 | 4 | 465 | 3 | 410 | 7.03e-149 | 433 |
| MS.gene60312.t1 | AT3G28570 | 47.639 | 466 | 194 | 6 | 6 | 461 | 7 | 432 | 5.18e-146 | 427 |
| MS.gene60312.t1 | AT3G28570 | 47.639 | 466 | 194 | 6 | 6 | 461 | 14 | 439 | 8.67e-146 | 426 |
| MS.gene60312.t1 | AT5G57480 | 42.474 | 485 | 248 | 10 | 1 | 470 | 1 | 469 | 7.66e-122 | 367 |
| MS.gene60312.t1 | AT3G50940 | 42.257 | 452 | 217 | 8 | 20 | 465 | 31 | 444 | 3.59e-121 | 363 |
| MS.gene60312.t1 | AT4G25835 | 42.794 | 451 | 232 | 8 | 1 | 442 | 1 | 434 | 4.57e-121 | 365 |
| MS.gene60312.t1 | AT3G50930 | 41.434 | 502 | 258 | 9 | 16 | 511 | 73 | 544 | 2.51e-120 | 365 |
| MS.gene60312.t1 | AT4G30250 | 41.393 | 488 | 256 | 9 | 1 | 475 | 1 | 471 | 4.44e-112 | 342 |
| MS.gene60312.t1 | AT4G30250 | 41.393 | 488 | 256 | 9 | 1 | 475 | 3 | 473 | 5.60e-112 | 342 |
| MS.gene60312.t1 | AT2G18193 | 39.425 | 487 | 251 | 11 | 10 | 489 | 19 | 468 | 6.15e-109 | 333 |
| MS.gene60312.t1 | AT5G17760 | 39.909 | 441 | 209 | 7 | 64 | 484 | 72 | 476 | 7.71e-103 | 318 |
| MS.gene60312.t1 | AT2G18190 | 38.085 | 470 | 253 | 8 | 2 | 467 | 13 | 448 | 5.01e-102 | 315 |
| MS.gene60312.t1 | AT5G17740 | 36.276 | 521 | 274 | 9 | 4 | 510 | 13 | 489 | 4.21e-101 | 314 |
| MS.gene60312.t1 | AT4G05380 | 37.179 | 468 | 245 | 11 | 10 | 465 | 20 | 450 | 1.18e-98 | 305 |
| MS.gene60312.t1 | AT5G17730 | 38.983 | 413 | 207 | 7 | 64 | 467 | 72 | 448 | 3.88e-96 | 299 |
| MS.gene60312.t1 | AT1G43910 | 37.527 | 461 | 250 | 10 | 16 | 468 | 26 | 456 | 1.30e-92 | 290 |
| MS.gene60312.t1 | AT5G17750 | 37.182 | 433 | 208 | 8 | 4 | 427 | 13 | 390 | 9.82e-91 | 283 |
| MS.gene60312.t1 | AT2G46620 | 32.334 | 467 | 258 | 8 | 1 | 461 | 1 | 415 | 1.27e-74 | 244 |
| MS.gene60312.t1 | AT5G17760 | 39.179 | 268 | 139 | 2 | 64 | 316 | 72 | 330 | 1.28e-57 | 195 |
| MS.gene60312.t1 | AT3G28560 | 41.395 | 215 | 116 | 3 | 4 | 215 | 7 | 214 | 2.36e-51 | 176 |
| MS.gene60312.t1 | AT3G29800 | 30.890 | 382 | 198 | 10 | 104 | 479 | 76 | 397 | 1.29e-46 | 168 |
| MS.gene60312.t1 | AT4G05340 | 48.889 | 90 | 38 | 1 | 333 | 414 | 4 | 93 | 3.73e-20 | 85.9 |
| MS.gene60312.t1 | AT5G15250 | 28.079 | 203 | 118 | 7 | 203 | 403 | 222 | 398 | 4.53e-12 | 68.9 |
| MS.gene60312.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 203 | 410 | 225 | 412 | 6.93e-12 | 68.6 |
| MS.gene60312.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 203 | 410 | 225 | 412 | 6.93e-12 | 68.6 |
| MS.gene60312.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 203 | 410 | 225 | 412 | 6.93e-12 | 68.6 |
| MS.gene60312.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 203 | 410 | 229 | 416 | 7.20e-12 | 68.6 |
| MS.gene60312.t1 | AT5G15250 | 28.866 | 194 | 110 | 7 | 203 | 394 | 222 | 389 | 1.58e-11 | 67.0 |
| MS.gene60312.t1 | AT1G06430 | 27.586 | 203 | 119 | 7 | 203 | 403 | 218 | 394 | 1.87e-11 | 67.0 |
| MS.gene60312.t1 | AT1G06430 | 27.586 | 203 | 119 | 7 | 203 | 403 | 218 | 394 | 1.87e-11 | 67.0 |
| MS.gene60312.t1 | AT1G06430 | 27.586 | 203 | 119 | 7 | 203 | 403 | 218 | 394 | 1.87e-11 | 67.0 |
| MS.gene60312.t1 | AT5G20000 | 27.919 | 197 | 114 | 6 | 202 | 395 | 158 | 329 | 6.36e-11 | 64.7 |
| MS.gene60312.t1 | AT5G19990 | 27.919 | 197 | 114 | 6 | 202 | 395 | 158 | 329 | 6.53e-11 | 64.7 |
| MS.gene60312.t1 | AT5G19990 | 27.919 | 197 | 114 | 6 | 202 | 395 | 158 | 329 | 6.53e-11 | 64.7 |
| MS.gene60312.t1 | AT5G19990 | 27.919 | 197 | 114 | 6 | 202 | 395 | 100 | 271 | 6.74e-11 | 64.3 |
Find 87 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAGCAAAGTGACACTTTC+AGG | 0.150044 | 8.2:-18691474 | MS.gene60312:CDS |
| TACAATAACAGGTTCATATT+TGG | 0.168811 | 8.2:-18692057 | MS.gene60312:CDS |
| TTCTCTTGCTCTTGATAATA+AGG | 0.193736 | 8.2:+18692119 | None:intergenic |
| AAAGAGTAGTGGTTTAATTA+AGG | 0.254123 | 8.2:-18691097 | MS.gene60312:CDS |
| GAAAGAGATTATTGAAGATT+TGG | 0.268353 | 8.2:-18691865 | MS.gene60312:CDS |
| GATTGTTCACTTGATCTTAC+AGG | 0.280503 | 8.2:-18691609 | MS.gene60312:CDS |
| TGAATTTCATTGATGGAATA+TGG | 0.281171 | 8.2:-18691446 | MS.gene60312:CDS |
| TAGCAAGTTTCATGTTCATT+TGG | 0.286672 | 8.2:-18692472 | MS.gene60312:CDS |
| GTTGGTCCTATTAAGGAAAA+TGG | 0.291304 | 8.2:-18691051 | MS.gene60312:CDS |
| ATAAAGGTGTCAAGGTTTAT+TGG | 0.304406 | 8.2:-18692178 | MS.gene60312:CDS |
| TGCTGCTATGGCTAATTTGT+TGG | 0.304769 | 8.2:-18691739 | MS.gene60312:CDS |
| GGAACATGTTGGTCCTATTA+AGG | 0.313426 | 8.2:-18691058 | MS.gene60312:CDS |
| GAGAGTATTGAGAAGCTAAT+TGG | 0.324876 | 8.2:-18691246 | MS.gene60312:CDS |
| ATAATCTCTTTCTTCTTCTC+AGG | 0.331857 | 8.2:+18691876 | None:intergenic |
| ATCTCATTCCATGAATTCTT+AGG | 0.343436 | 8.2:-18692356 | MS.gene60312:CDS |
| AGCAAGTTTCATGTTCATTT+GGG | 0.345653 | 8.2:-18692471 | MS.gene60312:CDS |
| TGATGATGAGAATGAAAATA+AGG | 0.348942 | 8.2:-18691550 | MS.gene60312:CDS |
| TAAAGGTGTCAAGGTTTATT+GGG | 0.349534 | 8.2:-18692177 | MS.gene60312:CDS |
| TGCTTGCTAATAATTACTTA+AGG | 0.358552 | 8.2:-18691290 | MS.gene60312:CDS |
| GTCTGTCACCTAAGAATTCA+TGG | 0.358843 | 8.2:+18692348 | None:intergenic |
| GCTTGCTAATAATTACTTAA+GGG | 0.363741 | 8.2:-18691289 | MS.gene60312:CDS |
| AGCTTGATCCTGCACTTATA+AGG | 0.377934 | 8.2:-18691371 | MS.gene60312:CDS |
| GCCATTGTTTCAAAAGTTGC+AGG | 0.380424 | 8.2:+18691906 | None:intergenic |
| GGGCTATTGAATTTCATTGA+TGG | 0.383780 | 8.2:-18691453 | MS.gene60312:CDS |
| GCTGCTATGGCTAATTTGTT+GGG | 0.384550 | 8.2:-18691738 | MS.gene60312:CDS |
| CACATAGTTTGCTTATAGCT+TGG | 0.413731 | 8.2:+18691951 | None:intergenic |
| ATCAACAATGATTGCTGCTA+TGG | 0.421593 | 8.2:-18691751 | MS.gene60312:CDS |
| GGGTTATGATGTTTATGATT+TGG | 0.423960 | 8.2:-18691718 | MS.gene60312:CDS |
| AGGTGAAGGAAGAAGGAAGT+GGG | 0.424613 | 8.2:-18691503 | MS.gene60312:CDS |
| AGGAAGGAGGTGAAGGAAGA+AGG | 0.430248 | 8.2:-18691510 | MS.gene60312:CDS |
| TTTCAAAGAAACGTAGAAGT+TGG | 0.437255 | 8.2:+18692423 | None:intergenic |
| AAGAGACTCAAAGCTGAGAT+TGG | 0.441694 | 8.2:-18692260 | MS.gene60312:CDS |
| TGGAATGAGATTCTTATGTA+AGG | 0.443190 | 8.2:+18692368 | None:intergenic |
| AAGTATAGAGATACAATAAC+AGG | 0.445450 | 8.2:-18692068 | MS.gene60312:CDS |
| AGAGTAACTGATGATTATAA+AGG | 0.449383 | 8.2:-18692194 | MS.gene60312:CDS |
| GAATCGGAAAAGCTTGGAAA+AGG | 0.463614 | 8.2:-18691809 | MS.gene60312:CDS |
| TTGGATCATGTGATGAAAGA+AGG | 0.464493 | 8.2:-18692038 | MS.gene60312:CDS |
| GAGGTGAAGGAAGAAGGAAG+TGG | 0.470118 | 8.2:-18691504 | MS.gene60312:CDS |
| AAGGAAAATGGTGAGGTTGT+TGG | 0.472585 | 8.2:-18691039 | MS.gene60312:CDS |
| ATGCTAGAATCGGAAAAGCT+TGG | 0.480606 | 8.2:-18691815 | MS.gene60312:CDS |
| CTCAAAAGAAGTGATGCTTA+TGG | 0.482161 | 8.2:-18692326 | MS.gene60312:CDS |
| GTGAAGGAAGAAGGAAGTGG+GGG | 0.488319 | 8.2:-18691501 | MS.gene60312:CDS |
| ACTGAAATGTGGACAACAAT+GGG | 0.488365 | 8.2:-18692503 | MS.gene60312:CDS |
| TGGAAAAGATAGCACCAACT+TGG | 0.490393 | 8.2:-18692240 | MS.gene60312:CDS |
| CAAACAGTCCTGGTTACAAA+TGG | 0.491886 | 8.2:-18691974 | MS.gene60312:CDS |
| TGGATCATGTGATGAAAGAA+GGG | 0.494339 | 8.2:-18692037 | MS.gene60312:CDS |
| AATCGGAAAAGCTTGGAAAA+GGG | 0.498865 | 8.2:-18691808 | MS.gene60312:CDS |
| TACTCTTTCATATTCATCCA+TGG | 0.501783 | 8.2:+18692211 | None:intergenic |
| AAAATACAGTCACAGAATCA+TGG | 0.502989 | 8.2:-18692402 | MS.gene60312:CDS |
| ATTGTTGATTTACCAGTACC+TGG | 0.512918 | 8.2:+18691765 | None:intergenic |
| TGCTGTGAGGAAGGAGGTGA+AGG | 0.515156 | 8.2:-18691517 | MS.gene60312:CDS |
| CTACCCCTCCTTATAAGTGC+AGG | 0.519905 | 8.2:+18691363 | None:intergenic |
| ACTTATAAGGAGGGGTAGAA+TGG | 0.522112 | 8.2:-18691358 | MS.gene60312:CDS |
| AAGGGAAAGAGATTCGTTTG+AGG | 0.523702 | 8.2:-18692019 | MS.gene60312:CDS |
| AAGAAACGTAGAAGTTGGTA+TGG | 0.524293 | 8.2:+18692428 | None:intergenic |
| GGTGAAGGAAGAAGGAAGTG+GGG | 0.528598 | 8.2:-18691502 | MS.gene60312:CDS |
| AGATTCTCAGCAACATCAGC+AGG | 0.533263 | 8.2:+18691207 | None:intergenic |
| TAAGGAACAAGATGAGGAAG+TGG | 0.537409 | 8.2:-18691079 | MS.gene60312:CDS |
| GCATCATCCATTGGAGACTT+AGG | 0.537455 | 8.2:+18691180 | None:intergenic |
| TTTAATTAAGGAACAAGATG+AGG | 0.541321 | 8.2:-18691085 | MS.gene60312:CDS |
| CACTTATCAGCATCATCCAT+TGG | 0.541958 | 8.2:+18691171 | None:intergenic |
| CACTTGATCTTACAGGTCAA+AGG | 0.546145 | 8.2:-18691602 | MS.gene60312:CDS |
| ATAGCAAAGTGACACTTTCA+GGG | 0.551851 | 8.2:-18691473 | MS.gene60312:CDS |
| ATCCATGGTTAAAACCAAGT+TGG | 0.556634 | 8.2:+18692226 | None:intergenic |
| TAGCTTGGCCATTTGTAACC+AGG | 0.556681 | 8.2:+18691966 | None:intergenic |
| TTTGGAGTTAACAGCAGTGA+AGG | 0.569744 | 8.2:-18691700 | MS.gene60312:CDS |
| TCTTATGCCTAAGTCTCCAA+TGG | 0.574824 | 8.2:-18691187 | MS.gene60312:CDS |
| ATCGGAAAAGCTTGGAAAAG+GGG | 0.576254 | 8.2:-18691807 | MS.gene60312:CDS |
| AATGAACATGAAACTTGCTA+AGG | 0.578436 | 8.2:+18692475 | None:intergenic |
| CCTACAAAATGACTGAAATG+TGG | 0.583765 | 8.2:-18692514 | None:intergenic |
| TGATCCTGCACTTATAAGGA+GGG | 0.596373 | 8.2:-18691367 | MS.gene60312:CDS |
| TGATGATTATAAAGGTGTCA+AGG | 0.596926 | 8.2:-18692186 | MS.gene60312:CDS |
| TTGCTCTTGATAATAAGGCA+TGG | 0.598551 | 8.2:+18692124 | None:intergenic |
| TTGATCCTGCACTTATAAGG+AGG | 0.600419 | 8.2:-18691368 | MS.gene60312:CDS |
| GACTGAAATGTGGACAACAA+TGG | 0.606751 | 8.2:-18692504 | MS.gene60312:CDS |
| TCCTATTAAGGAAAATGGTG+AGG | 0.608727 | 8.2:-18691046 | MS.gene60312:CDS |
| AAATTGTATACAAACAGTCC+TGG | 0.610851 | 8.2:-18691984 | MS.gene60312:CDS |
| TTCGTTTGAGGAACAGACAG+AGG | 0.628162 | 8.2:-18692007 | MS.gene60312:CDS |
| AAGCAATGAAGCAAATTCAG+AGG | 0.635171 | 8.2:-18690996 | MS.gene60312:CDS |
| GGTCAGCTTGTGGAGGTGAA+AGG | 0.651822 | 8.2:-18691425 | MS.gene60312:CDS |
| GATGAGGAAGTGGAACATGT+TGG | 0.656984 | 8.2:-18691069 | MS.gene60312:CDS |
| TGAAGGATAATACTGAGTTG+AGG | 0.661198 | 8.2:-18691683 | MS.gene60312:CDS |
| GATGGAATATGGTCAGCTTG+TGG | 0.665052 | 8.2:-18691435 | MS.gene60312:CDS |
| GATCCTGCACTTATAAGGAG+GGG | 0.671043 | 8.2:-18691366 | MS.gene60312:CDS |
| CAAGCTATAAGCAAACTATG+TGG | 0.703587 | 8.2:-18691950 | MS.gene60312:CDS |
| GGAATATGGTCAGCTTGTGG+AGG | 0.710003 | 8.2:-18691432 | MS.gene60312:CDS |
| GTTGATTTACCAGTACCTGG+AGG | 0.741136 | 8.2:+18691768 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTCTTATTGTAGTTTTAA+TGG | - | chr8.2:18692178-18692197 | MS.gene60312:CDS | 20.0% |
| ! | AAAGAGTAGTGGTTTAATTA+AGG | - | chr8.2:18692402-18692421 | MS.gene60312:CDS | 25.0% |
| ! | AAGTATAGAGATACAATAAC+AGG | - | chr8.2:18691431-18691450 | MS.gene60312:CDS | 25.0% |
| ! | AGAGTAACTGATGATTATAA+AGG | - | chr8.2:18691305-18691324 | MS.gene60312:CDS | 25.0% |
| ! | GAAAGAGATTATTGAAGATT+TGG | - | chr8.2:18691634-18691653 | MS.gene60312:CDS | 25.0% |
| ! | TACAATAACAGGTTCATATT+TGG | - | chr8.2:18691442-18691461 | MS.gene60312:CDS | 25.0% |
| ! | TGAATTTCATTGATGGAATA+TGG | - | chr8.2:18692053-18692072 | MS.gene60312:CDS | 25.0% |
| ! | TTTAATTAAGGAACAAGATG+AGG | - | chr8.2:18692414-18692433 | MS.gene60312:CDS | 25.0% |
| !! | AAGGATTTTTATGCTAGAAT+CGG | - | chr8.2:18691674-18691693 | MS.gene60312:CDS | 25.0% |
| !! | GCTTGCTAATAATTACTTAA+GGG | - | chr8.2:18692210-18692229 | MS.gene60312:CDS | 25.0% |
| !! | TGATGATGAGAATGAAAATA+AGG | - | chr8.2:18691949-18691968 | MS.gene60312:CDS | 25.0% |
| !! | TGCTTGCTAATAATTACTTA+AGG | - | chr8.2:18692209-18692228 | MS.gene60312:CDS | 25.0% |
| !! | TGTTTTCACAACAAATTATG+TGG | - | chr8.2:18692102-18692121 | MS.gene60312:CDS | 25.0% |
| !!! | AAAAGGGGTTATTTACTTTT+TGG | - | chr8.2:18691707-18691726 | MS.gene60312:CDS | 25.0% |
| !!! | GGTTACTTTTAGTAAAAGTA+AGG | - | chr8.2:18691655-18691674 | MS.gene60312:CDS | 25.0% |
| AAAATACAGTCACAGAATCA+TGG | - | chr8.2:18691097-18691116 | MS.gene60312:CDS | 30.0% | |
| AAATTGTATACAAACAGTCC+TGG | - | chr8.2:18691515-18691534 | MS.gene60312:CDS | 30.0% | |
| AGCAAGTTTCATGTTCATTT+GGG | - | chr8.2:18691028-18691047 | MS.gene60312:CDS | 30.0% | |
| ATAATCTCTTTCTTCTTCTC+AGG | + | chr8.2:18691626-18691645 | None:intergenic | 30.0% | |
| ATCTCATTCCATGAATTCTT+AGG | - | chr8.2:18691143-18691162 | MS.gene60312:CDS | 30.0% | |
| GGGTTATGATGTTTATGATT+TGG | - | chr8.2:18691781-18691800 | MS.gene60312:CDS | 30.0% | |
| TACTCTTTCATATTCATCCA+TGG | + | chr8.2:18691291-18691310 | None:intergenic | 30.0% | |
| TAGCAAGTTTCATGTTCATT+TGG | - | chr8.2:18691027-18691046 | MS.gene60312:CDS | 30.0% | |
| TGGAATGAGATTCTTATGTA+AGG | + | chr8.2:18691134-18691153 | None:intergenic | 30.0% | |
| TTTCAAAGAAACGTAGAAGT+TGG | + | chr8.2:18691079-18691098 | None:intergenic | 30.0% | |
| ! | TTCTCTTGCTCTTGATAATA+AGG | + | chr8.2:18691383-18691402 | None:intergenic | 30.0% |
| !! | AATGAACATGAAACTTGCTA+AGG | + | chr8.2:18691027-18691046 | None:intergenic | 30.0% |
| !! | ATAAAGGTGTCAAGGTTTAT+TGG | - | chr8.2:18691321-18691340 | MS.gene60312:CDS | 30.0% |
| !! | TAAAGGTGTCAAGGTTTATT+GGG | - | chr8.2:18691322-18691341 | MS.gene60312:CDS | 30.0% |
| !! | TGATGATTATAAAGGTGTCA+AGG | - | chr8.2:18691313-18691332 | MS.gene60312:CDS | 30.0% |
| !!! | AACTTTTGAAACAATGGCTA+TGG | - | chr8.2:18691598-18691617 | MS.gene60312:CDS | 30.0% |
| AAGCAATGAAGCAAATTCAG+AGG | - | chr8.2:18692503-18692522 | MS.gene60312:CDS | 35.0% | |
| AATAGCAAAGTGACACTTTC+AGG | - | chr8.2:18692025-18692044 | MS.gene60312:CDS | 35.0% | |
| AATCGGAAAAGCTTGGAAAA+GGG | - | chr8.2:18691691-18691710 | MS.gene60312:CDS | 35.0% | |
| ACAGGTCAAAGGAAGAAAAA+AGG | - | chr8.2:18691908-18691927 | MS.gene60312:CDS | 35.0% | |
| ACTGAAATGTGGACAACAAT+GGG | - | chr8.2:18690996-18691015 | MS.gene60312:CDS | 35.0% | |
| ATAGCAAAGTGACACTTTCA+GGG | - | chr8.2:18692026-18692045 | MS.gene60312:CDS | 35.0% | |
| ATCAACAATGATTGCTGCTA+TGG | - | chr8.2:18691748-18691767 | MS.gene60312:CDS | 35.0% | |
| ATCCATGGTTAAAACCAAGT+TGG | + | chr8.2:18691276-18691295 | None:intergenic | 35.0% | |
| CAAGCTATAAGCAAACTATG+TGG | - | chr8.2:18691549-18691568 | MS.gene60312:CDS | 35.0% | |
| CTCAAAAGAAGTGATGCTTA+TGG | - | chr8.2:18691173-18691192 | MS.gene60312:CDS | 35.0% | |
| GATTGTTCACTTGATCTTAC+AGG | - | chr8.2:18691890-18691909 | MS.gene60312:CDS | 35.0% | |
| GGGCTATTGAATTTCATTGA+TGG | - | chr8.2:18692046-18692065 | MS.gene60312:CDS | 35.0% | |
| GTTGGTCCTATTAAGGAAAA+TGG | - | chr8.2:18692448-18692467 | MS.gene60312:CDS | 35.0% | |
| TCCTATTAAGGAAAATGGTG+AGG | - | chr8.2:18692453-18692472 | MS.gene60312:CDS | 35.0% | |
| TCTTGCTGATAAAAAAGCTG+AGG | - | chr8.2:18692372-18692391 | MS.gene60312:CDS | 35.0% | |
| TGAAGGATAATACTGAGTTG+AGG | - | chr8.2:18691816-18691835 | MS.gene60312:CDS | 35.0% | |
| TGGATCATGTGATGAAAGAA+GGG | - | chr8.2:18691462-18691481 | MS.gene60312:CDS | 35.0% | |
| TTGGATCATGTGATGAAAGA+AGG | - | chr8.2:18691461-18691480 | MS.gene60312:CDS | 35.0% | |
| ! | ACCTCACCATTTTCCTTAAT+AGG | + | chr8.2:18692457-18692476 | None:intergenic | 35.0% |
| ! | TTGCTCTTGATAATAAGGCA+TGG | + | chr8.2:18691378-18691397 | None:intergenic | 35.0% |
| !! | AAGAAACGTAGAAGTTGGTA+TGG | + | chr8.2:18691074-18691093 | None:intergenic | 35.0% |
| !! | ATTGTTGATTTACCAGTACC+TGG | + | chr8.2:18691737-18691756 | None:intergenic | 35.0% |
| !! | CACATAGTTTGCTTATAGCT+TGG | + | chr8.2:18691551-18691570 | None:intergenic | 35.0% |
| !! | GAGAGTATTGAGAAGCTAAT+TGG | - | chr8.2:18692253-18692272 | MS.gene60312:CDS | 35.0% |
| !! | TAATTTTGATGCTGTGAGGA+AGG | - | chr8.2:18691973-18691992 | MS.gene60312:CDS | 35.0% |
| !!! | TATTTACTTTTTGGTCCTCC+AGG | - | chr8.2:18691716-18691735 | MS.gene60312:CDS | 35.0% |
| !!! | TCCTGCAACTTTTGAAACAA+TGG | - | chr8.2:18691592-18691611 | MS.gene60312:CDS | 35.0% |
| AAGAGACTCAAAGCTGAGAT+TGG | - | chr8.2:18691239-18691258 | MS.gene60312:CDS | 40.0% | |
| AAGGAAAATGGTGAGGTTGT+TGG | - | chr8.2:18692460-18692479 | MS.gene60312:CDS | 40.0% | |
| AAGGGAAAGAGATTCGTTTG+AGG | - | chr8.2:18691480-18691499 | MS.gene60312:CDS | 40.0% | |
| ACTTATAAGGAGGGGTAGAA+TGG | - | chr8.2:18692141-18692160 | MS.gene60312:CDS | 40.0% | |
| AGCTTGATCCTGCACTTATA+AGG | - | chr8.2:18692128-18692147 | MS.gene60312:CDS | 40.0% | |
| ATCGGAAAAGCTTGGAAAAG+GGG | - | chr8.2:18691692-18691711 | MS.gene60312:CDS | 40.0% | |
| ATGCTAGAATCGGAAAAGCT+TGG | - | chr8.2:18691684-18691703 | MS.gene60312:CDS | 40.0% | |
| CAAACAGTCCTGGTTACAAA+TGG | - | chr8.2:18691525-18691544 | MS.gene60312:CDS | 40.0% | |
| CACTTATCAGCATCATCCAT+TGG | + | chr8.2:18692331-18692350 | None:intergenic | 40.0% | |
| CACTTGATCTTACAGGTCAA+AGG | - | chr8.2:18691897-18691916 | MS.gene60312:CDS | 40.0% | |
| GAATCGGAAAAGCTTGGAAA+AGG | - | chr8.2:18691690-18691709 | MS.gene60312:CDS | 40.0% | |
| GACTGAAATGTGGACAACAA+TGG | - | chr8.2:18690995-18691014 | MS.gene60312:CDS | 40.0% | |
| GCCATTGTTTCAAAAGTTGC+AGG | + | chr8.2:18691596-18691615 | None:intergenic | 40.0% | |
| GGAACATGTTGGTCCTATTA+AGG | - | chr8.2:18692441-18692460 | MS.gene60312:CDS | 40.0% | |
| GTCTGTCACCTAAGAATTCA+TGG | + | chr8.2:18691154-18691173 | None:intergenic | 40.0% | |
| TAAGGAACAAGATGAGGAAG+TGG | - | chr8.2:18692420-18692439 | MS.gene60312:CDS | 40.0% | |
| TCTTATGCCTAAGTCTCCAA+TGG | - | chr8.2:18692312-18692331 | MS.gene60312:CDS | 40.0% | |
| TGATCCTGCACTTATAAGGA+GGG | - | chr8.2:18692132-18692151 | MS.gene60312:CDS | 40.0% | |
| TGCTGATAAAAAAGCTGAGG+AGG | - | chr8.2:18692375-18692394 | MS.gene60312:CDS | 40.0% | |
| TGGAAAAGATAGCACCAACT+TGG | - | chr8.2:18691259-18691278 | MS.gene60312:CDS | 40.0% | |
| TTGATCCTGCACTTATAAGG+AGG | - | chr8.2:18692131-18692150 | MS.gene60312:CDS | 40.0% | |
| ! | TGCTGCTATGGCTAATTTGT+TGG | - | chr8.2:18691760-18691779 | MS.gene60312:CDS | 40.0% |
| ! | TTTGGAGTTAACAGCAGTGA+AGG | - | chr8.2:18691799-18691818 | MS.gene60312:CDS | 40.0% |
| !! | AGGCTAATTTTGATGCTGTG+AGG | - | chr8.2:18691969-18691988 | MS.gene60312:CDS | 40.0% |
| !! | GCTGCTATGGCTAATTTGTT+GGG | - | chr8.2:18691761-18691780 | MS.gene60312:CDS | 40.0% |
| !!! | CACCAACTTGGTTTTAACCA+TGG | - | chr8.2:18691271-18691290 | MS.gene60312:CDS | 40.0% |
| AGATTCTCAGCAACATCAGC+AGG | + | chr8.2:18692295-18692314 | None:intergenic | 45.0% | |
| AGGTGAAGGAAGAAGGAAGT+GGG | - | chr8.2:18691996-18692015 | MS.gene60312:CDS | 45.0% | |
| GAGGAGGTGAAAAAGAGTAG+TGG | - | chr8.2:18692391-18692410 | MS.gene60312:CDS | 45.0% | |
| GATCCTGCACTTATAAGGAG+GGG | - | chr8.2:18692133-18692152 | MS.gene60312:CDS | 45.0% | |
| GATGAGGAAGTGGAACATGT+TGG | - | chr8.2:18692430-18692449 | MS.gene60312:CDS | 45.0% | |
| GATGGAATATGGTCAGCTTG+TGG | - | chr8.2:18692064-18692083 | MS.gene60312:CDS | 45.0% | |
| GCATCATCCATTGGAGACTT+AGG | + | chr8.2:18692322-18692341 | None:intergenic | 45.0% | |
| TAGCTTGGCCATTTGTAACC+AGG | + | chr8.2:18691536-18691555 | None:intergenic | 45.0% | |
| TTCGTTTGAGGAACAGACAG+AGG | - | chr8.2:18691492-18691511 | MS.gene60312:CDS | 45.0% | |
| ! | GTTGATTTACCAGTACCTGG+AGG | + | chr8.2:18691734-18691753 | None:intergenic | 45.0% |
| ! | TTTTGATGCTGTGAGGAAGG+AGG | - | chr8.2:18691976-18691995 | MS.gene60312:CDS | 45.0% |
| AGGAAGGAGGTGAAGGAAGA+AGG | - | chr8.2:18691989-18692008 | MS.gene60312:CDS | 50.0% | |
| CTACCCCTCCTTATAAGTGC+AGG | + | chr8.2:18692139-18692158 | None:intergenic | 50.0% | |
| GAGGTGAAGGAAGAAGGAAG+TGG | - | chr8.2:18691995-18692014 | MS.gene60312:CDS | 50.0% | |
| GGAATATGGTCAGCTTGTGG+AGG | - | chr8.2:18692067-18692086 | MS.gene60312:CDS | 50.0% | |
| GGTGAAGGAAGAAGGAAGTG+GGG | - | chr8.2:18691997-18692016 | MS.gene60312:CDS | 50.0% | |
| GTGAAGGAAGAAGGAAGTGG+GGG | - | chr8.2:18691998-18692017 | MS.gene60312:CDS | 50.0% | |
| !! | CTTTTTGGTCCTCCAGGTAC+TGG | - | chr8.2:18691722-18691741 | MS.gene60312:CDS | 50.0% |
| AAGGAAGAAGGAAGTGGGGG+TGG | - | chr8.2:18692001-18692020 | MS.gene60312:CDS | 55.0% | |
| AGGAAGAAGGAAGTGGGGGT+GGG | - | chr8.2:18692002-18692021 | MS.gene60312:CDS | 55.0% | |
| GGTCAGCTTGTGGAGGTGAA+AGG | - | chr8.2:18692074-18692093 | MS.gene60312:CDS | 55.0% | |
| TGCTGTGAGGAAGGAGGTGA+AGG | - | chr8.2:18691982-18692001 | MS.gene60312:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 18690993 | 18692528 | 18690993 | ID=MS.gene60312 |
| chr8.2 | mRNA | 18690993 | 18692528 | 18690993 | ID=MS.gene60312.t1;Parent=MS.gene60312 |
| chr8.2 | exon | 18690993 | 18692528 | 18690993 | ID=MS.gene60312.t1.exon1;Parent=MS.gene60312.t1 |
| chr8.2 | CDS | 18690993 | 18692528 | 18690993 | ID=cds.MS.gene60312.t1;Parent=MS.gene60312.t1 |
| Gene Sequence |
| Protein sequence |
