Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060359.t1 | RHN64206.1 | 96.7 | 892 | 25 | 2 | 1 | 892 | 44 | 931 | 0.00E+00 | 1739.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060359.t1 | B1KHA5 | 28.0 | 264 | 120 | 7 | 31 | 292 | 5 | 200 | 1.3e-11 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060359.t1 | G7JJ08 | 96.7 | 892 | 25 | 2 | 1 | 892 | 1 | 888 | 0.0e+00 | 1739.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050894 | MS.gene060359 | 0.805886 | 1.07E-49 | -1.69E-46 |
| MS.gene060359 | MS.gene072351 | 0.82582 | 3.80E-54 | -1.69E-46 |
| MS.gene060359 | MS.gene072905 | 0.800385 | 1.48E-48 | -1.69E-46 |
| MS.gene060359 | MS.gene20033 | 0.819844 | 9.38E-53 | -1.69E-46 |
| MS.gene060359 | MS.gene32620 | 0.804792 | 1.82E-49 | -1.69E-46 |
| MS.gene060359 | MS.gene32853 | 0.821758 | 3.40E-53 | -1.69E-46 |
| MS.gene060359 | MS.gene35102 | 0.810977 | 8.81E-51 | -1.69E-46 |
| MS.gene060359 | MS.gene37459 | 0.824388 | 8.29E-54 | -1.69E-46 |
| MS.gene060359 | MS.gene41692 | 0.816706 | 4.82E-52 | -1.69E-46 |
| MS.gene060359 | MS.gene43869 | 0.807364 | 5.24E-50 | -1.69E-46 |
| MS.gene060359 | MS.gene44024 | 0.809823 | 1.56E-50 | -1.69E-46 |
| MS.gene060359 | MS.gene52975 | 0.803416 | 3.52E-49 | -1.69E-46 |
| MS.gene060359 | MS.gene58096 | 0.821782 | 3.36E-53 | -1.69E-46 |
| MS.gene060359 | MS.gene60312 | 0.811679 | 6.20E-51 | -1.69E-46 |
| MS.gene060359 | MS.gene60537 | 0.831205 | 1.90E-55 | -1.69E-46 |
| MS.gene060359 | MS.gene60539 | 0.821251 | 4.46E-53 | -1.69E-46 |
| MS.gene060359 | MS.gene62172 | 0.841457 | 4.70E-58 | -1.69E-46 |
| MS.gene060359 | MS.gene62896 | 0.831881 | 1.30E-55 | -1.69E-46 |
| MS.gene060359 | MS.gene63290 | 0.833711 | 4.56E-56 | -1.69E-46 |
| MS.gene060359 | MS.gene63504 | 0.834734 | 2.53E-56 | -1.69E-46 |
| MS.gene060359 | MS.gene64077 | 0.807656 | 4.54E-50 | -1.69E-46 |
| MS.gene060359 | MS.gene66159 | 0.811667 | 6.24E-51 | -1.69E-46 |
| MS.gene060359 | MS.gene68060 | 0.823394 | 1.42E-53 | -1.69E-46 |
| MS.gene060359 | MS.gene70240 | 0.812252 | 4.66E-51 | -1.69E-46 |
| MS.gene060359 | MS.gene70898 | 0.805859 | 1.09E-49 | -1.69E-46 |
| MS.gene060359 | MS.gene71941 | 0.805354 | 1.39E-49 | -1.69E-46 |
| MS.gene060359 | MS.gene72170 | 0.806477 | 8.07E-50 | -1.69E-46 |
| MS.gene060359 | MS.gene83234 | 0.812361 | 4.41E-51 | -1.69E-46 |
| MS.gene060359 | MS.gene94573 | 0.836774 | 7.68E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060359.t1 | MTR_4g118590 | 96.749 | 892 | 25 | 2 | 1 | 892 | 1 | 888 | 0.0 | 1790 |
| MS.gene060359.t1 | MTR_4g118590 | 97.404 | 809 | 21 | 0 | 84 | 892 | 1 | 809 | 0.0 | 1647 |
| MS.gene060359.t1 | MTR_4g118590 | 30.650 | 323 | 163 | 13 | 26 | 326 | 415 | 698 | 1.33e-29 | 127 |
| MS.gene060359.t1 | MTR_4g118570 | 81.439 | 862 | 105 | 3 | 31 | 892 | 43 | 849 | 0.0 | 1432 |
| MS.gene060359.t1 | MTR_2g020750 | 76.871 | 882 | 196 | 4 | 1 | 879 | 1 | 877 | 0.0 | 1367 |
| MS.gene060359.t1 | MTR_2g020750 | 75.964 | 882 | 194 | 5 | 1 | 879 | 1 | 867 | 0.0 | 1342 |
| MS.gene060359.t1 | MTR_2g020750 | 78.294 | 797 | 167 | 3 | 84 | 879 | 1 | 792 | 0.0 | 1303 |
| MS.gene060359.t1 | MTR_2g020750 | 30.719 | 306 | 150 | 11 | 24 | 308 | 410 | 674 | 8.53e-30 | 127 |
| MS.gene060359.t1 | MTR_2g020760 | 70.370 | 351 | 87 | 2 | 530 | 879 | 207 | 541 | 3.09e-168 | 500 |
| MS.gene060359.t1 | MTR_2g020760 | 81.250 | 176 | 32 | 1 | 18 | 192 | 8 | 183 | 1.69e-76 | 259 |
| MS.gene060359.t1 | MTR_2g020760 | 27.338 | 278 | 127 | 11 | 60 | 314 | 207 | 432 | 8.99e-18 | 88.2 |
| MS.gene060359.t1 | MTR_2g020760 | 32.000 | 175 | 81 | 8 | 528 | 685 | 48 | 201 | 5.62e-13 | 72.8 |
| MS.gene060359.t1 | MTR_1g007110 | 59.016 | 122 | 35 | 2 | 640 | 761 | 1 | 107 | 8.10e-38 | 137 |
| MS.gene060359.t1 | MTR_7g014365 | 65.455 | 55 | 19 | 0 | 747 | 801 | 289 | 343 | 4.80e-14 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060359.t1 | AT5G18860 | 66.170 | 872 | 287 | 5 | 14 | 877 | 17 | 888 | 0.0 | 1192 |
| MS.gene060359.t1 | AT5G18860 | 66.170 | 872 | 287 | 5 | 14 | 877 | 17 | 888 | 0.0 | 1192 |
| MS.gene060359.t1 | AT5G18890 | 61.152 | 538 | 204 | 4 | 352 | 887 | 14 | 548 | 0.0 | 688 |
| MS.gene060359.t1 | AT5G18890 | 30.625 | 320 | 161 | 11 | 15 | 313 | 149 | 428 | 3.29e-31 | 130 |
| MS.gene060359.t1 | AT5G18870 | 63.670 | 267 | 76 | 4 | 4 | 269 | 5 | 251 | 6.32e-108 | 333 |
| MS.gene060359.t1 | AT5G18870 | 35.470 | 234 | 109 | 10 | 512 | 725 | 42 | 253 | 8.62e-25 | 105 |
Find 208 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGGGCTTCACCTCAAATTT+TGG | 0.133915 | 8.4:-12916269 | None:intergenic |
| ATCGATTCCGACACTCTTTA+TGG | 0.143772 | 8.4:+12914613 | MS.gene060359:CDS |
| ATTATCTCCTTTGTGATTAT+TGG | 0.164889 | 8.4:-12912970 | None:intergenic |
| ATTAGTACCTGTTCTATTAT+TGG | 0.193899 | 8.4:-12906546 | None:intergenic |
| TTATCTCCTTTGTGATTATT+GGG | 0.230423 | 8.4:-12912969 | None:intergenic |
| TATGCAGAATTCAATATCTT+TGG | 0.234222 | 8.4:+12912186 | MS.gene060359:CDS |
| TGTGGAACATATTTATATAA+TGG | 0.242560 | 8.4:+12912044 | MS.gene060359:CDS |
| GGCTCCAATCCATTATTTAA+TGG | 0.244145 | 8.4:+12913062 | MS.gene060359:CDS |
| ATGTTTGCAGGACACTTTCC+TGG | 0.248012 | 8.4:+12916196 | MS.gene060359:intron |
| TTTATATAGGTGATTCATTC+TGG | 0.249600 | 8.4:+12912581 | MS.gene060359:intron |
| GATCATGATACCAGGGAAAA+AGG | 0.254529 | 8.4:+12911881 | MS.gene060359:intron |
| TTAGATATTGATGCAAATTA+TGG | 0.259558 | 8.4:+12908910 | MS.gene060359:CDS |
| AGTTCATTTGCAACTATTAT+AGG | 0.268320 | 8.4:+12915953 | MS.gene060359:CDS |
| CATATGGTAATATCTGTTAT+TGG | 0.273254 | 8.4:-12916056 | None:intergenic |
| ACTTTCCTGGGTGAAATTCT+AGG | 0.275745 | 8.4:+12916209 | MS.gene060359:CDS |
| TGTATAGTAAGTCATAAATT+TGG | 0.300627 | 8.4:-12906431 | None:intergenic |
| CCTCTTTGCTTTGTGAAGAA+TGG | 0.301173 | 8.4:+12913158 | MS.gene060359:CDS |
| ACATGTTGAATTCTGCATAT+TGG | 0.301217 | 8.4:-12915847 | None:intergenic |
| TCCACCAACATTTGGGAGTA+TGG | 0.305702 | 8.4:-12906517 | None:intergenic |
| CCCAAGTTCAATCTCGAAAA+AGG | 0.307392 | 8.4:+12913095 | MS.gene060359:CDS |
| AACCACTAGCTATGGAAGTT+TGG | 0.317235 | 8.4:+12915086 | MS.gene060359:CDS |
| CCTCCAACAGCTCCGAATAT+TGG | 0.317457 | 8.4:-12914547 | None:intergenic |
| TTCTGATTCCAACACTGTTT+TGG | 0.318854 | 8.4:-12915888 | None:intergenic |
| TGAGGTGTGGATTATTCATT+AGG | 0.319796 | 8.4:-12912014 | None:intergenic |
| CAACACTTCACAATTTCAAT+TGG | 0.328503 | 8.4:+12904772 | MS.gene060359:CDS |
| TTGAAGAACAGACGAGGAAT+TGG | 0.336099 | 8.4:+12916456 | MS.gene060359:CDS |
| TTGATGCAAATTATGGTATC+AGG | 0.339395 | 8.4:+12908917 | MS.gene060359:CDS |
| GGTAGATATCCACCAACATT+TGG | 0.339504 | 8.4:-12906525 | None:intergenic |
| AGGAACTGTGATTTCTTTAG+TGG | 0.357382 | 8.4:+12904670 | MS.gene060359:CDS |
| AAATATAAGCTCTAGAATTC+AGG | 0.359345 | 8.4:+12915201 | MS.gene060359:CDS |
| ACTATTATAGGTAGATTAGA+AGG | 0.359872 | 8.4:+12915965 | MS.gene060359:CDS |
| AGATAATGAATTTGCTGAAA+TGG | 0.360031 | 8.4:+12912986 | MS.gene060359:CDS |
| GTTGGTTAGTACAGTAATAT+TGG | 0.363878 | 8.4:-12915140 | None:intergenic |
| GTGGAACATATTTATATAAT+GGG | 0.373048 | 8.4:+12912045 | MS.gene060359:CDS |
| GATGATATTGCTGTTGGAAT+TGG | 0.375044 | 8.4:+12906465 | MS.gene060359:CDS |
| GTAAGTTACTTCTTTGTAGT+AGG | 0.377745 | 8.4:-12913687 | None:intergenic |
| CCACTAGCTAAGACTTTAAC+GGG | 0.381874 | 8.4:-12916287 | None:intergenic |
| GGAGAAAACTCTGTGAAGTT+TGG | 0.384074 | 8.4:+12915028 | MS.gene060359:CDS |
| AATTCTGCATATTGGTTTGA+AGG | 0.384962 | 8.4:-12915839 | None:intergenic |
| TTCTTCAAAGCTTCCTACCT+TGG | 0.386548 | 8.4:-12916441 | None:intergenic |
| ACCACTAGCTATGGAAGTTT+GGG | 0.398336 | 8.4:+12915087 | MS.gene060359:CDS |
| GCAATCATTGTGAGTCCTAC+TGG | 0.403689 | 8.4:+12914415 | MS.gene060359:CDS |
| GCTAGTGGTTGTCTAAGTTC+AGG | 0.406726 | 8.4:-12915073 | None:intergenic |
| GTTCGACAACGAATTCTATT+CGG | 0.406887 | 8.4:+12912745 | MS.gene060359:CDS |
| ACAAGTACCCTTACTGAGTC+TGG | 0.412101 | 8.4:-12913439 | None:intergenic |
| AGAGCAACCAACTGCTCAAC+AGG | 0.415239 | 8.4:+12911918 | MS.gene060359:CDS |
| TGAAAATATCTCGTGATACT+TGG | 0.415655 | 8.4:+12912723 | MS.gene060359:CDS |
| AAGATATTGAATTCTGCATA+AGG | 0.415808 | 8.4:-12912183 | None:intergenic |
| CAGGCCATTCCCGTAGGACT+TGG | 0.424788 | 8.4:+12908880 | MS.gene060359:CDS |
| AAGTCTGAATCCAAAATTTG+AGG | 0.425233 | 8.4:+12916259 | MS.gene060359:CDS |
| TGGTTTGCTTCAAACGGTTT+AGG | 0.425581 | 8.4:-12916036 | None:intergenic |
| GTAGATATCCACCAACATTT+GGG | 0.428530 | 8.4:-12906524 | None:intergenic |
| GCAAATAGCTGGACTAGTGC+TGG | 0.429924 | 8.4:+12906396 | MS.gene060359:CDS |
| AATCAGAGAGATCCAATATT+CGG | 0.430677 | 8.4:+12914535 | MS.gene060359:CDS |
| TGTTCAAGTAATCGACCTTA+AGG | 0.431076 | 8.4:+12913811 | MS.gene060359:CDS |
| TTAAGGTCGATTACTTGAAC+AGG | 0.436542 | 8.4:-12913809 | None:intergenic |
| CAACGGACCCTTGACTAACT+TGG | 0.436573 | 8.4:+12915159 | MS.gene060359:CDS |
| GAGCCATCAGATATTCCATA+TGG | 0.441356 | 8.4:-12913044 | None:intergenic |
| ATTAAGCAATGTGGAGGAAA+AGG | 0.442657 | 8.4:+12916370 | MS.gene060359:CDS |
| GTGCAGTAGTCTTGGCTGAT+AGG | 0.443525 | 8.4:+12916231 | MS.gene060359:CDS |
| GCCACTAGCTAAGACTTTAA+CGG | 0.443932 | 8.4:-12916288 | None:intergenic |
| TGTTATTGGTTTGCTTCAAA+CGG | 0.450871 | 8.4:-12916042 | None:intergenic |
| ATTACTTCAAATAAACCATA+TGG | 0.452481 | 8.4:+12913029 | MS.gene060359:CDS |
| TGCTTTGTGAAGAATGGAAA+AGG | 0.452550 | 8.4:+12913164 | MS.gene060359:CDS |
| CACTTCACAATTTCAATTGG+AGG | 0.455153 | 8.4:+12904775 | MS.gene060359:CDS |
| TAAGTTACTTCTTTGTAGTA+GGG | 0.458308 | 8.4:-12913686 | None:intergenic |
| GTGAAGTGTTGAGTTTAAGA+AGG | 0.459630 | 8.4:-12904760 | None:intergenic |
| CTCTTGGTTAACACAACCTC+TGG | 0.461666 | 8.4:-12916001 | None:intergenic |
| TAGGCCATTAAATAATGGAT+TGG | 0.462074 | 8.4:-12913066 | None:intergenic |
| AATCAGGGAATGACAACAAC+AGG | 0.465338 | 8.4:+12908841 | MS.gene060359:intron |
| AAGCACCTTCTTGGGCTTCG+TGG | 0.471386 | 8.4:-12914652 | None:intergenic |
| CAACAGGAGGCTGCAGATAC+AGG | 0.471451 | 8.4:+12908857 | MS.gene060359:CDS |
| TAGGCTTGACAATAGACTCT+TGG | 0.471773 | 8.4:-12916017 | None:intergenic |
| ATGTTGGGAGTTCACTTGAC+AGG | 0.473138 | 8.4:+12913488 | MS.gene060359:CDS |
| TGTTTGCAGGACACTTTCCT+GGG | 0.476236 | 8.4:+12916197 | MS.gene060359:intron |
| AGATATTGAATTCTGCATAA+GGG | 0.477716 | 8.4:-12912182 | None:intergenic |
| CTTGAACAGGCACTTTAAGA+AGG | 0.479468 | 8.4:-12913796 | None:intergenic |
| ATCAACACCTGTTGAGCAGT+TGG | 0.480240 | 8.4:-12911925 | None:intergenic |
| GCAAAGCCTAACCGAGATGT+TGG | 0.482388 | 8.4:+12913472 | MS.gene060359:CDS |
| TGACCATCAGTGCAAATAGC+TGG | 0.486563 | 8.4:+12906385 | MS.gene060359:CDS |
| ATATAATGGGTGGAGGTGTA+AGG | 0.487070 | 8.4:+12912058 | MS.gene060359:CDS |
| AAAACGTCTCCTAGACCAAC+TGG | 0.490973 | 8.4:-12914505 | None:intergenic |
| TCCCACAACTCTGTTTATGA+TGG | 0.491961 | 8.4:+12911966 | MS.gene060359:CDS |
| CCAATATTCGGAGCTGTTGG+AGG | 0.494618 | 8.4:+12914547 | MS.gene060359:CDS |
| CTAGTGGTTGTCTAAGTTCA+GGG | 0.496809 | 8.4:-12915072 | None:intergenic |
| AACCGTCCTCCAAGTCCTAC+GGG | 0.497663 | 8.4:-12908889 | None:intergenic |
| GATGATATACCAGTTGGTCT+AGG | 0.500674 | 8.4:+12914496 | MS.gene060359:CDS |
| GCCATTCCCGTAGGACTTGG+AGG | 0.501855 | 8.4:+12908883 | MS.gene060359:CDS |
| AACTGTGATTTCTTTAGTGG+AGG | 0.502703 | 8.4:+12904673 | MS.gene060359:CDS |
| TAGGTGGTCCAGACTCAGTA+AGG | 0.505627 | 8.4:+12913431 | MS.gene060359:CDS |
| GTTGCGGCCACTCTTAACCT+TGG | 0.505742 | 8.4:-12916526 | None:intergenic |
| ATGTCCATGTCAAACACAAC+AGG | 0.506003 | 8.4:-12913743 | None:intergenic |
| CAAAGCCTAACCGAGATGTT+GGG | 0.507521 | 8.4:+12913473 | MS.gene060359:CDS |
| ATCAAAGAATTGTTCAGTGA+TGG | 0.507773 | 8.4:-12912648 | None:intergenic |
| CTTGTTGAATATGACCACTA+TGG | 0.508643 | 8.4:-12913124 | None:intergenic |
| TTATGACTTACTATACATGA+TGG | 0.508788 | 8.4:+12906437 | MS.gene060359:CDS |
| GTTTGGAGCCCCACGGGATA+CGG | 0.508801 | 8.4:+12915045 | MS.gene060359:CDS |
| GGGAGAGATGATATTGCTGT+TGG | 0.509268 | 8.4:+12906459 | MS.gene060359:CDS |
| AATTCTAGGTGCAGTAGTCT+TGG | 0.512169 | 8.4:+12916223 | MS.gene060359:CDS |
| TGAGCAGTTGGTTGCTCTAG+TGG | 0.512644 | 8.4:-12911913 | None:intergenic |
| TCCTCCAAGTCCTACGGGAA+TGG | 0.512927 | 8.4:-12908884 | None:intergenic |
| TCAAGAGGAACTAGGGTGAC+AGG | 0.515905 | 8.4:-12912608 | None:intergenic |
| TATGACTTACTATACATGAT+GGG | 0.517827 | 8.4:+12906438 | MS.gene060359:CDS |
| TATGTTAAAACCATTCCTCA+TGG | 0.518173 | 8.4:+12914577 | MS.gene060359:CDS |
| CCCATCATAAACAGAGTTGT+GGG | 0.518573 | 8.4:-12911967 | None:intergenic |
| ACTGTGATTTCTTTAGTGGA+GGG | 0.519542 | 8.4:+12904674 | MS.gene060359:CDS |
| CAATAACAGATATTACCATA+TGG | 0.519571 | 8.4:+12916057 | MS.gene060359:CDS |
| TCAAAGAATTGTTCAGTGAT+GGG | 0.520811 | 8.4:-12912647 | None:intergenic |
| AGTCCAGCTATTTGCACTGA+TGG | 0.522005 | 8.4:-12906388 | None:intergenic |
| GAATGGAAAAGGAAAATGTC+AGG | 0.523227 | 8.4:+12913175 | MS.gene060359:CDS |
| TTATGACATACTTCACATGA+TGG | 0.524848 | 8.4:+12914468 | MS.gene060359:CDS |
| ATATCTACCAATAATAGAAC+AGG | 0.527403 | 8.4:+12906539 | MS.gene060359:CDS |
| GATCCAATATTCGGAGCTGT+TGG | 0.528960 | 8.4:+12914544 | MS.gene060359:CDS |
| TAACCGTCCTCCAAGTCCTA+CGG | 0.534528 | 8.4:-12908890 | None:intergenic |
| ACTTAGACAACCACTAGCTA+TGG | 0.534826 | 8.4:+12915078 | MS.gene060359:CDS |
| CAGGGTGATCCGTATCCCGT+GGG | 0.535686 | 8.4:-12915054 | None:intergenic |
| GTTGGAATTGGTGGCGAGGG+TGG | 0.536758 | 8.4:+12906477 | MS.gene060359:CDS |
| AACGTTTGCCAAGTTAGTCA+AGG | 0.540677 | 8.4:-12915167 | None:intergenic |
| CCATTCTTCACAAAGCAAAG+AGG | 0.541179 | 8.4:-12913158 | None:intergenic |
| ACCTCCATTCCCATGAGGAA+TGG | 0.541814 | 8.4:-12914587 | None:intergenic |
| GCTGCATTTGCCCATCCAGT+AGG | 0.543690 | 8.4:-12914430 | None:intergenic |
| ATAGGATGGTTACACAAAAG+AGG | 0.548092 | 8.4:+12913408 | MS.gene060359:intron |
| ATGACCTGGTAAGCTGCAAA+AGG | 0.548275 | 8.4:-12912213 | None:intergenic |
| GTAAGGTCAAGCAATCCAAC+TGG | 0.548736 | 8.4:+12912075 | MS.gene060359:CDS |
| AATCTGTAAACATATTTCCA+TGG | 0.549731 | 8.4:-12912149 | None:intergenic |
| AATATGTACGCAAACAAGCT+TGG | 0.550403 | 8.4:+12916401 | MS.gene060359:CDS |
| ACTTGGCGCTGAGTACTCAA+TGG | 0.553468 | 8.4:-12915929 | None:intergenic |
| TGTGAAGTTTGGAGCCCCAC+GGG | 0.554758 | 8.4:+12915039 | MS.gene060359:CDS |
| TCTGAGGATGTTCCTGCGGA+GGG | 0.556719 | 8.4:+12904613 | None:intergenic |
| TCCCATCATAAACAGAGTTG+TGG | 0.556830 | 8.4:-12911968 | None:intergenic |
| TTTAGCCGCCAAAACAGTGT+TGG | 0.556946 | 8.4:+12915880 | MS.gene060359:CDS |
| CTAAAGCAACCACGACAAGC+AGG | 0.557424 | 8.4:+12913638 | MS.gene060359:CDS |
| GTCTGAGGATGTTCCTGCGG+AGG | 0.557466 | 8.4:+12904612 | None:intergenic |
| GCTGTTGGAATTGGTGGCGA+GGG | 0.559333 | 8.4:+12906474 | MS.gene060359:CDS |
| TCATTGTGAGTCCTACTGGA+TGG | 0.559692 | 8.4:+12914419 | MS.gene060359:CDS |
| CAGGAAAGCTTTCCTCCCAC+AGG | 0.560341 | 8.4:+12908936 | MS.gene060359:CDS |
| GAGGGTGGAATACTGTCAAA+TGG | 0.561194 | 8.4:+12906492 | MS.gene060359:CDS |
| TTCCCGTAGGACTTGGAGGA+CGG | 0.562952 | 8.4:+12908887 | MS.gene060359:CDS |
| CAAGTTAGTCAAGGGTCCGT+TGG | 0.564476 | 8.4:-12915158 | None:intergenic |
| CATATTTATATAATGGGTGG+AGG | 0.565672 | 8.4:+12912051 | MS.gene060359:CDS |
| AAAACCATTCCTCATGGGAA+TGG | 0.566418 | 8.4:+12914583 | MS.gene060359:CDS |
| AAACCATATGGAATATCTGA+TGG | 0.570401 | 8.4:+12913041 | MS.gene060359:CDS |
| CAGCTGCAGCTACCCTCCGC+AGG | 0.570459 | 8.4:-12904625 | None:intergenic |
| AGTGGCATCGAATCCACTGA+TGG | 0.571465 | 8.4:+12916305 | MS.gene060359:CDS |
| ACCATTCCTCATGGGAATGG+AGG | 0.571467 | 8.4:+12914586 | MS.gene060359:CDS |
| CAAATAGCTGGACTAGTGCT+GGG | 0.573476 | 8.4:+12906397 | MS.gene060359:CDS |
| TGATGCATCAAGAGGAACTA+GGG | 0.574582 | 8.4:-12912615 | None:intergenic |
| TCAGGGTGATCCGTATCCCG+TGG | 0.576268 | 8.4:-12915055 | None:intergenic |
| CAAAGAATTGTTCAGTGATG+GGG | 0.577275 | 8.4:-12912646 | None:intergenic |
| TTGATGCATCAAGAGGAACT+AGG | 0.577314 | 8.4:-12912616 | None:intergenic |
| CATTGTGAGTCCTACTGGAT+GGG | 0.577818 | 8.4:+12914420 | MS.gene060359:CDS |
| GATATTGCTGTTGGAATTGG+TGG | 0.578658 | 8.4:+12906468 | MS.gene060359:CDS |
| TATGACATACTTCACATGAT+GGG | 0.580387 | 8.4:+12914469 | MS.gene060359:CDS |
| ACCATACTCCCAAATGTTGG+TGG | 0.580841 | 8.4:+12906516 | MS.gene060359:CDS |
| AGGAGGCTGCAGATACAGGC+AGG | 0.582786 | 8.4:+12908861 | MS.gene060359:CDS |
| CTGTGAAGTTTGGAGCCCCA+CGG | 0.585376 | 8.4:+12915038 | MS.gene060359:CDS |
| CCCACAACTCTGTTTATGAT+GGG | 0.586502 | 8.4:+12911967 | MS.gene060359:CDS |
| TTGTATCTCCCTGCTTGTCG+TGG | 0.586926 | 8.4:-12913647 | None:intergenic |
| GAACTCCCAACATCTCGGTT+AGG | 0.595333 | 8.4:-12913478 | None:intergenic |
| GAACATATTTATATAATGGG+TGG | 0.596251 | 8.4:+12912048 | MS.gene060359:CDS |
| CAGAATATTAAGCAATGTGG+AGG | 0.596519 | 8.4:+12916364 | MS.gene060359:CDS |
| TGCTGTTGGAATTGGTGGCG+AGG | 0.598178 | 8.4:+12906473 | MS.gene060359:CDS |
| CCCGTTAAAGTCTTAGCTAG+TGG | 0.599921 | 8.4:+12916287 | MS.gene060359:CDS |
| CAAGTGAACTCCCAACATCT+CGG | 0.600515 | 8.4:-12913483 | None:intergenic |
| ACGTTTGCCAAGTTAGTCAA+GGG | 0.602796 | 8.4:-12915166 | None:intergenic |
| ATGTTAAAACCATTCCTCAT+GGG | 0.608056 | 8.4:+12914578 | MS.gene060359:CDS |
| GTATTGCAAACAATGAAACC+AGG | 0.608873 | 8.4:+12915115 | MS.gene060359:CDS |
| ATCTGCCACGAAGCCCAAGA+AGG | 0.610019 | 8.4:+12914647 | MS.gene060359:CDS |
| TGGGTCTGAGGATGTTCCTG+CGG | 0.610076 | 8.4:+12904609 | None:intergenic |
| AATATTACTGTACTAACCAA+CGG | 0.611883 | 8.4:+12915142 | MS.gene060359:CDS |
| AGCAAGACCATAAAGAGTGT+CGG | 0.617790 | 8.4:-12914620 | None:intergenic |
| CTGTATCAGTCAGCCAAGGT+AGG | 0.617978 | 8.4:+12916428 | MS.gene060359:CDS |
| CCTCGACAGTGTGGTGACCA+TGG | 0.619192 | 8.4:+12912132 | MS.gene060359:CDS |
| TGTTGGGAGTTCACTTGACA+GGG | 0.619735 | 8.4:+12913489 | MS.gene060359:CDS |
| ATGACTTACTATACATGATG+GGG | 0.623093 | 8.4:+12906439 | MS.gene060359:CDS |
| AAAACCTGTTGTGTTTGACA+TGG | 0.625184 | 8.4:+12913739 | MS.gene060359:CDS |
| GGTCGTGATGATATACCAGT+TGG | 0.625658 | 8.4:+12914490 | MS.gene060359:CDS |
| AGTACAGTAATATTGGACCC+TGG | 0.625884 | 8.4:-12915133 | None:intergenic |
| GGTACCATACTCCCAAATGT+TGG | 0.629801 | 8.4:+12906513 | MS.gene060359:CDS |
| TAAAGCAACCACGACAAGCA+GGG | 0.630858 | 8.4:+12913639 | MS.gene060359:CDS |
| GATGGTTACACAAAAGAGGT+AGG | 0.631222 | 8.4:+12913412 | MS.gene060359:CDS |
| ATTGTGTTTGATGCATCAAG+AGG | 0.634012 | 8.4:-12912623 | None:intergenic |
| AAAGCCTCATCGAATCGTTG+TGG | 0.634864 | 8.4:+12904697 | MS.gene060359:CDS |
| GCGTTATTAATACCTGTGGG+AGG | 0.634886 | 8.4:-12908948 | None:intergenic |
| TCCCAAACTTCCATAGCTAG+TGG | 0.639935 | 8.4:-12915088 | None:intergenic |
| CTATTATAGGTAGATTAGAA+GGG | 0.641988 | 8.4:+12915966 | MS.gene060359:CDS |
| ACCACACTGTCGAGGCACAC+AGG | 0.642222 | 8.4:-12912124 | None:intergenic |
| CATAGTGGTCATATTCAACA+AGG | 0.643535 | 8.4:+12913125 | MS.gene060359:CDS |
| AGTAAACCCAATAATCACAA+AGG | 0.646458 | 8.4:+12912963 | MS.gene060359:CDS |
| TAGTTGCAAATGAACTCACT+TGG | 0.647844 | 8.4:-12915946 | None:intergenic |
| TTTGACATGGACATGAGTGC+AGG | 0.649664 | 8.4:+12913752 | MS.gene060359:CDS |
| CCATGGTCACCACACTGTCG+AGG | 0.655257 | 8.4:-12912132 | None:intergenic |
| CGATCTGTATCAGTCAGCCA+AGG | 0.661216 | 8.4:+12916424 | MS.gene060359:CDS |
| AGTCAGAATATTAAGCAATG+TGG | 0.665146 | 8.4:+12916361 | MS.gene060359:CDS |
| AGCAGTAATGCAGAAGACAA+AGG | 0.665677 | 8.4:+12915800 | MS.gene060359:CDS |
| ACCTGTGTGCCTCGACAGTG+TGG | 0.670023 | 8.4:+12912123 | MS.gene060359:CDS |
| CAGGGAATGACAACAACAGG+AGG | 0.676364 | 8.4:+12908844 | MS.gene060359:intron |
| ATAGCGGTAGATGAGAAACA+TGG | 0.678340 | 8.4:+12916332 | MS.gene060359:CDS |
| TATTGCAAACAATGAAACCA+GGG | 0.678984 | 8.4:+12915116 | MS.gene060359:CDS |
| AGGTGGTCCAGACTCAGTAA+GGG | 0.681287 | 8.4:+12913432 | MS.gene060359:CDS |
| TACAGGCAGGCCATTCCCGT+AGG | 0.682638 | 8.4:+12908874 | MS.gene060359:CDS |
| GTATCCACAACGATTCGATG+AGG | 0.695732 | 8.4:-12904701 | None:intergenic |
| CTGCACCTAGAATTTCACCC+AGG | 0.696015 | 8.4:-12916214 | None:intergenic |
| GGTTACACAAAAGAGGTAGG+TGG | 0.708754 | 8.4:+12913415 | MS.gene060359:CDS |
| AATTGGAGTCATCCTCACGA+TGG | 0.723657 | 8.4:+12916473 | MS.gene060359:CDS |
| ATGTAACCTCCATTCCCATG+AGG | 0.729135 | 8.4:-12914592 | None:intergenic |
| AGGGTGATCCGTATCCCGTG+GGG | 0.734001 | 8.4:-12915053 | None:intergenic |
| GAAGCTTTGAAGAACAGACG+AGG | 0.750728 | 8.4:+12916450 | MS.gene060359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 12904620 | 12916535 | 12904620 | ID=MS.gene060359 |
| chr8.4 | mRNA | 12904620 | 12916535 | 12904620 | ID=MS.gene060359.t1;Parent=MS.gene060359 |
| chr8.4 | exon | 12904620 | 12904796 | 12904620 | ID=MS.gene060359.t1.exon1;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12904620 | 12904796 | 12904620 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12906381 | 12906560 | 12906381 | ID=MS.gene060359.t1.exon2;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12906381 | 12906560 | 12906381 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12908847 | 12908957 | 12908847 | ID=MS.gene060359.t1.exon3;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12908847 | 12908957 | 12908847 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12911895 | 12912230 | 12911895 | ID=MS.gene060359.t1.exon4;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12911895 | 12912230 | 12911895 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12912590 | 12912766 | 12912590 | ID=MS.gene060359.t1.exon5;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12912590 | 12912766 | 12912590 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12912912 | 12913196 | 12912912 | ID=MS.gene060359.t1.exon6;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12912912 | 12913196 | 12912912 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12913412 | 12913534 | 12913412 | ID=MS.gene060359.t1.exon7;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12913412 | 12913534 | 12913412 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12913635 | 12913832 | 12913635 | ID=MS.gene060359.t1.exon8;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12913635 | 12913832 | 12913635 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12914415 | 12914668 | 12914415 | ID=MS.gene060359.t1.exon9;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12914415 | 12914668 | 12914415 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12915021 | 12915222 | 12915021 | ID=MS.gene060359.t1.exon10;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12915021 | 12915222 | 12915021 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12915773 | 12916078 | 12915773 | ID=MS.gene060359.t1.exon11;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12915773 | 12916078 | 12915773 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| chr8.4 | exon | 12916206 | 12916535 | 12916206 | ID=MS.gene060359.t1.exon12;Parent=MS.gene060359.t1 |
| chr8.4 | CDS | 12916206 | 12916535 | 12916206 | ID=cds.MS.gene060359.t1;Parent=MS.gene060359.t1 |
| Gene Sequence |
| Protein sequence |
