AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60686.t1 MTR_1g098680 78.618 304 18 4 1 304 1 257 2.88e-68 213
MS.gene60686.t1 MTR_6g037220 72.527 91 25 0 213 303 20 110 8.76e-19 80.5
MS.gene60686.t1 MTR_0583s0010 70.330 91 24 1 213 303 20 107 1.25e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 97 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGAAACACCATTTCTT+TGG 0.236527 1.2:-70772693 MS.gene60686:CDS
CCATGAGCATCATGAGAAAA+AGG 0.283151 1.2:-70772748 MS.gene60686:CDS
TTGTGCCTGTGAGCGTTCTC+TGG 0.290296 1.2:+70772825 None:intergenic
ACCACCACCGGTGGTGGTTT+TGG 0.301983 1.2:+70773998 None:intergenic
TCCGCCACCGGTGGTTGTTT+TGG 0.307495 1.2:+70774199 None:intergenic
ACAACCACCGGTGGCGGATA+TGG 0.322982 1.2:-70774194 MS.gene60686:CDS
GGCGGCTACGGTGGTGGATA+TGG 0.331422 1.2:-70774032 MS.gene60686:CDS
GGTGGCTACGGTGGTGGTTA+TGG 0.331534 1.2:-70774233 MS.gene60686:CDS
GGTGGCTACGGTGGTGGTTA+TGG 0.333221 1.2:-70774281 MS.gene60686:CDS
CATGAGCATCTTGGTGAGTT+AGG 0.335349 1.2:-70773915 MS.gene60686:CDS
GCCACCGCCAGTGGTTGTTT+TGG 0.336345 1.2:+70774298 None:intergenic
GGCGGCTACGGTGGTGGTTA+TGG 0.343719 1.2:-70774134 MS.gene60686:CDS
GGCGGATATGGTGGTGGTTA+TGG 0.349031 1.2:-70774182 MS.gene60686:CDS
ACAGGTGATGACTATGATTC+TGG 0.353087 1.2:-70774479 MS.gene60686:CDS
ACCACCACCGGTGGTGGTTA+TGG 0.357161 1.2:-70773993 MS.gene60686:CDS
GTAGCCACCACCAGTGGTTT+TGG 0.366481 1.2:+70774247 None:intergenic
TCATATGGTAATGACAATTC+TGG 0.390159 1.2:-70774395 MS.gene60686:CDS
GGCGGCTACAGTGGTGGTTA+TGG 0.393635 1.2:-70774083 MS.gene60686:CDS
GAAGCAGCTGCAGTGGGATC+TGG 0.415672 1.2:-70772783 MS.gene60686:CDS
TACTCTAATAATGAAACATC+TGG 0.429220 1.2:-70774332 MS.gene60686:CDS
AGTTACAATAAAACATCATA+TGG 0.441444 1.2:-70774410 MS.gene60686:CDS
TCTGGTGGTGACTATGAAAC+TGG 0.450209 1.2:-70774377 MS.gene60686:CDS
TAACCACTGTCAGTGTCTAT+TGG 0.452120 1.2:+70774524 None:intergenic
CATATGTTCATCCAAAGAAA+TGG 0.455940 1.2:+70772682 None:intergenic
GAGTTAGGTGCTGCAGCTGC+CGG 0.467505 1.2:-70773900 MS.gene60686:CDS
TACAATGATGACCAGCCATC+TGG 0.471386 1.2:-70774437 MS.gene60686:CDS
ACTGGAGGCGGCTACGGTGG+TGG 0.471438 1.2:-70774038 MS.gene60686:CDS
GGAAACAAAGGAGGAAGAAG+AGG 0.476387 1.2:-70772727 MS.gene60686:CDS
ACAACCACTGGCGGTGGCTA+CGG 0.494222 1.2:-70774293 MS.gene60686:CDS
ACCAAAACAACCACCGGTGG+CGG 0.496756 1.2:-70774200 MS.gene60686:CDS
CACAGCAAACATGAGCATCT+TGG 0.497752 1.2:-70773924 MS.gene60686:CDS
AGTTGCAGAAGCAGCTGCAG+TGG 0.498157 1.2:-70772790 MS.gene60686:CDS
ACCAAAACCACCACCGGTGG+TGG 0.498197 1.2:-70773999 MS.gene60686:CDS
ACTGGCGGTGGCTACGGTGG+TGG 0.498849 1.2:-70774287 MS.gene60686:CDS
ACTGGTGGTGGCTACGGTGG+TGG 0.506091 1.2:-70774239 MS.gene60686:CDS
CATCATCATGCTTCTTGTGA+TGG 0.509136 1.2:+70774558 None:intergenic
TGTGCCTGTGAGCGTTCTCT+GGG 0.510366 1.2:+70772826 None:intergenic
ACCACTGGCGGCTACGGTGG+TGG 0.510905 1.2:-70774140 MS.gene60686:CDS
ACCATAACCACCACCGGTGG+TGG 0.516051 1.2:+70773992 None:intergenic
ACAACCACTGGAGGCGGCTA+CGG 0.519220 1.2:-70774044 MS.gene60686:CDS
AAAACATCATCATACTCTAC+AGG 0.520952 1.2:-70774497 MS.gene60686:CDS
GACACCAAAACCACTGGTGG+TGG 0.521255 1.2:-70774251 MS.gene60686:CDS
GCCAAAACAACCACTGGCGG+TGG 0.530730 1.2:-70774299 MS.gene60686:CDS
AAAACCACTGGTGGTGGCTA+CGG 0.535946 1.2:-70774245 MS.gene60686:CDS
GCAGCTGCAGTGGGATCTGG+TGG 0.536922 1.2:-70772780 MS.gene60686:CDS
AATGAAACATCTGGTGGCTA+TGG 0.540775 1.2:-70774323 MS.gene60686:CDS
ACTAAAACAACCACTGGTGG+CGG 0.542116 1.2:-70774101 MS.gene60686:CDS
ACCACTGGTGGCGGCTACAG+TGG 0.542206 1.2:-70774092 MS.gene60686:CDS
GATGGAAGAGGCCACGGCTA+CGG 0.545278 1.2:+70774576 None:intergenic
GACTCTGACACCAAAACCAC+TGG 0.545560 1.2:-70774257 MS.gene60686:CDS
GTTGCAGAAGCAGCTGCAGT+GGG 0.546096 1.2:-70772789 MS.gene60686:CDS
TTAGGTGCTGCAGCTGCCGG+CGG 0.547760 1.2:-70773897 MS.gene60686:CDS
TTTCTCATGATGCTCATGGA+AGG 0.551185 1.2:+70772752 None:intergenic
TGTAACTACCACCAGATGGC+TGG 0.558526 1.2:+70774426 None:intergenic
TATGGTGCCAAAACAACCAC+TGG 0.560714 1.2:-70774305 MS.gene60686:CDS
ACCACCGGTGGCGGATATGG+TGG 0.560863 1.2:-70774191 MS.gene60686:CDS
ACCGGTGGCGGATATGGTGG+TGG 0.561478 1.2:-70774188 MS.gene60686:CDS
ACTAAAACAACCACTGGAGG+CGG 0.561518 1.2:-70774050 MS.gene60686:CDS
ACCACTGGAGGCGGCTACGG+TGG 0.561554 1.2:-70774041 MS.gene60686:CDS
CATGCTTCTTGTGATGGAAG+AGG 0.565809 1.2:+70774564 None:intergenic
TCTGACACTAAAACAACCAC+TGG 0.570147 1.2:-70774056 MS.gene60686:CDS
ACCACTGGCGGTGGCTACGG+TGG 0.575237 1.2:-70774290 MS.gene60686:CDS
ACCACTGGTGGTGGCTACGG+TGG 0.575768 1.2:-70774242 MS.gene60686:CDS
ACCACCGTAGCCGCCTCCAG+TGG 0.576587 1.2:+70774040 None:intergenic
GAGGAAGAAGAGGAAGCTCA+TGG 0.577274 1.2:-70772717 MS.gene60686:CDS
AAGACCCAGAGAACGCTCAC+AGG 0.577440 1.2:-70772830 MS.gene60686:CDS
TCTGACACTAAAACAACCAC+TGG 0.583853 1.2:-70774107 MS.gene60686:CDS
ACCACCACCATATCCGCCAC+CGG 0.592287 1.2:+70774187 None:intergenic
ACCACCGTAGCCACCGCCAG+TGG 0.595656 1.2:+70774289 None:intergenic
TCTGACACCAAAACCACCAC+CGG 0.597416 1.2:-70774005 MS.gene60686:CDS
TCTGACACCAAAACAACCAC+CGG 0.597416 1.2:-70774206 MS.gene60686:CDS
GACACTAAAACAACCACTGG+TGG 0.602888 1.2:-70774104 MS.gene60686:CDS
TTATTGTAACTACCACCAGA+TGG 0.604027 1.2:+70774422 None:intergenic
AATACCACTGGCGGCTACGG+TGG 0.604479 1.2:-70774143 MS.gene60686:CDS
ACCACTGTAGCCGCCACCAG+TGG 0.604950 1.2:+70774091 None:intergenic
CAGCTGCAGTGGGATCTGGT+GGG 0.605425 1.2:-70772779 MS.gene60686:CDS
TCTTGTGATGGAAGAGGCCA+CGG 0.606734 1.2:+70774570 None:intergenic
ATCGTCACCATAACCACCAC+CGG 0.607906 1.2:+70773986 None:intergenic
ACCACCATATCCGCCACCGG+TGG 0.608942 1.2:+70774190 None:intergenic
TCTAATAATGAAACATCTGG+TGG 0.616695 1.2:-70774329 MS.gene60686:CDS
ACGGTTCTCTTCATCAGACA+TGG 0.625188 1.2:+70774595 None:intergenic
GACACTAAAACAACCACTGG+AGG 0.626777 1.2:-70774053 MS.gene60686:CDS
GACACCAAAACCACCACCGG+TGG 0.634543 1.2:-70774002 MS.gene60686:CDS
GATGAAGAGAACCGTAGCCG+TGG 0.635065 1.2:-70774587 MS.gene60686:CDS
ACTGGTGGCGGCTACAGTGG+TGG 0.637908 1.2:-70774089 MS.gene60686:CDS
AATTACCAAAGCAAAACCGC+CGG 0.640922 1.2:+70773881 None:intergenic
ACCACCGTAGCCACCACCAG+TGG 0.645976 1.2:+70774241 None:intergenic
CAAGATGCTCATGTTTGCTG+TGG 0.649171 1.2:+70773925 None:intergenic
ACCACCACCGTAGCCGCCAG+TGG 0.650364 1.2:+70774139 None:intergenic
TCTGACACCAAAACCACTGG+TGG 0.658393 1.2:-70774254 MS.gene60686:CDS
AATGATGACCAGCCATCTGG+TGG 0.661265 1.2:-70774434 MS.gene60686:CDS
GACACCAAAACAACCACCGG+TGG 0.679833 1.2:-70774203 MS.gene60686:CDS
GGTGCCAAAACAACCACTGG+CGG 0.690106 1.2:-70774302 MS.gene60686:CDS
GTCACCATAACCACCACCGG+TGG 0.707664 1.2:+70773989 None:intergenic
TATGGTAATGACAATTCTGG+TGG 0.722206 1.2:-70774392 MS.gene60686:CDS
AGACGTTGATTATAAGAACG+AGG 0.726638 1.2:-70773955 MS.gene60686:CDS
AGACCAATAGACACTGACAG+TGG 0.753613 1.2:-70774527 MS.gene60686:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTAATTACAAAGTT+TGG + chr1.2:70774083-70774102 None:intergenic 10.0%
!!! TACATTTCAATTTTTTATTT+TGG + chr1.2:70774230-70774249 None:intergenic 10.0%
!! AGAAACAAATAACAAAAATA+TGG + chr1.2:70773468-70773487 None:intergenic 15.0%
!! GAAACAAATAACAAAAATAT+GGG + chr1.2:70773467-70773486 None:intergenic 15.0%
!! AGTTACAATAAAACATCATA+TGG - chr1.2:70772872-70772891 MS.gene60686:intron 20.0%
!!! AATCAATAAATTTTCTCTGA+TGG - chr1.2:70773518-70773537 MS.gene60686:intron 20.0%
!!! AATTATTCGAATGTTTTGTA+AGG + chr1.2:70773568-70773587 None:intergenic 20.0%
! GACTCATATTCATTAATAAG+TGG - chr1.2:70773898-70773917 MS.gene60686:CDS 25.0%
! TAAATAGCAATTAGGAGTAA+TGG + chr1.2:70773438-70773457 None:intergenic 25.0%
! TACTCTAATAATGAAACATC+TGG - chr1.2:70772950-70772969 MS.gene60686:intron 25.0%
! TGTAAAAATTCGATCTTGAT+TGG - chr1.2:70774246-70774265 MS.gene60686:CDS 25.0%
! TTAAATACTACAGTTCAGAT+TGG + chr1.2:70773973-70773992 None:intergenic 25.0%
! TTACGCTATAAATAGACATA+GGG - chr1.2:70774316-70774335 MS.gene60686:CDS 25.0%
! TTCCTATTATGGAAATCTTA+GGG + chr1.2:70774017-70774036 None:intergenic 25.0%
! TTTCCTATTATGGAAATCTT+AGG + chr1.2:70774018-70774037 None:intergenic 25.0%
AAAACATCATCATACTCTAC+AGG - chr1.2:70772785-70772804 MS.gene60686:CDS 30.0%
AAGAAGAAACACCATTTCTT+TGG - chr1.2:70774589-70774608 MS.gene60686:CDS 30.0%
AAGCCTATAACAAATACGAA+CGG - chr1.2:70774158-70774177 MS.gene60686:CDS 30.0%
AATGATTATCTCAAACATCG+TGG + chr1.2:70773942-70773961 None:intergenic 30.0%
GGAGTGATTAAATAGCAATT+AGG + chr1.2:70773446-70773465 None:intergenic 30.0%
GTTACGCTATAAATAGACAT+AGG - chr1.2:70774315-70774334 MS.gene60686:CDS 30.0%
TCATGAGAAAAAGGAAACAA+AGG - chr1.2:70774543-70774562 MS.gene60686:CDS 30.0%
TCTAATAATGAAACATCTGG+TGG - chr1.2:70772953-70772972 MS.gene60686:intron 30.0%
! TCATATGGTAATGACAATTC+TGG - chr1.2:70772887-70772906 MS.gene60686:intron 30.0%
! TTCACTAGATGTTAGTTTAG+TGG + chr1.2:70773693-70773712 None:intergenic 30.0%
! TTGGTGCAATTTTCCTATTA+TGG + chr1.2:70774028-70774047 None:intergenic 30.0%
!!! ACATGTTTTTTTGTAGAGTC+AGG - chr1.2:70774124-70774143 MS.gene60686:CDS 30.0%
AACATCCAAGCAAATCTGAA+CGG + chr1.2:70774273-70774292 None:intergenic 35.0%
TCTGACACTAAAAATACCAC+TGG - chr1.2:70773127-70773146 MS.gene60686:intron 35.0%
TGAGAAAAAGGAAACAAAGG+AGG - chr1.2:70774546-70774565 MS.gene60686:CDS 35.0%
TGTAATCTCCAACAACAAAG+TGG + chr1.2:70774051-70774070 None:intergenic 35.0%
TTATTGTAACTACCACCAGA+TGG + chr1.2:70772863-70772882 None:intergenic 35.0%
! AGACGTTGATTATAAGAACG+AGG - chr1.2:70773327-70773346 MS.gene60686:intron 35.0%
! AGATGTTAGTTTAGTGGTAC+TGG + chr1.2:70773687-70773706 None:intergenic 35.0%
! TATGGTAATGACAATTCTGG+TGG - chr1.2:70772890-70772909 MS.gene60686:intron 35.0%
!! TCACCGTTCGTATTTGTTAT+AGG + chr1.2:70774164-70774183 None:intergenic 35.0%
AATGAAACATCTGGTGGCTA+TGG - chr1.2:70772959-70772978 MS.gene60686:intron 40.0%
AATTACCAAAGCAAAACCGC+CGG + chr1.2:70773404-70773423 None:intergenic 40.0%
ACAGGTGATGACTATGATTC+TGG - chr1.2:70772803-70772822 MS.gene60686:CDS 40.0%
ACGAACGGTGAGATTAAGAT+CGG - chr1.2:70774173-70774192 MS.gene60686:CDS 40.0%
ATCTCCAACAACAAAGTGGT+TGG + chr1.2:70774047-70774066 None:intergenic 40.0%
CATCATCATGCTTCTTGTGA+TGG + chr1.2:70772727-70772746 None:intergenic 40.0%
CCATGAGCATCATGAGAAAA+AGG - chr1.2:70774534-70774553 MS.gene60686:CDS 40.0%
CGCCCTAAGATTTCCATAAT+AGG - chr1.2:70774012-70774031 MS.gene60686:CDS 40.0%
GACACTAAAAATACCACTGG+CGG - chr1.2:70773130-70773149 MS.gene60686:intron 40.0%
GAGTCATGCTCAACAAGATT+AGG + chr1.2:70773883-70773902 None:intergenic 40.0%
TAACCACTGTCAGTGTCTAT+TGG + chr1.2:70772761-70772780 None:intergenic 40.0%
TCTGACACTAAAACAACCAC+TGG - chr1.2:70773175-70773194 MS.gene60686:intron 40.0%
TTTCTCATGATGCTCATGGA+AGG + chr1.2:70774533-70774552 None:intergenic 40.0%
! AGATTTGCTTGGATGTTGGT+AGG - chr1.2:70774276-70774295 MS.gene60686:CDS 40.0%
! CCTTTTTCTCATGATGCTCA+TGG + chr1.2:70774537-70774556 None:intergenic 40.0%
! GTTCAGATTTGCTTGGATGT+TGG - chr1.2:70774272-70774291 MS.gene60686:CDS 40.0%
AAAAATACCACTGGCGGCTA+CGG - chr1.2:70773136-70773155 MS.gene60686:intron 45.0%
AAACATCGTGGAACGACTCA+AGG + chr1.2:70773930-70773949 None:intergenic 45.0%
ACTAAAACAACCACTGGAGG+CGG - chr1.2:70773232-70773251 MS.gene60686:intron 45.0%
ACTAAAACAACCACTGGTGG+CGG - chr1.2:70773181-70773200 MS.gene60686:intron 45.0%
AGACCAATAGACACTGACAG+TGG - chr1.2:70772755-70772774 MS.gene60686:CDS 45.0%
CACAGCAAACATGAGCATCT+TGG - chr1.2:70773358-70773377 MS.gene60686:intron 45.0%
CATGAGCATCTTGGTGAGTT+AGG - chr1.2:70773367-70773386 MS.gene60686:intron 45.0%
GACACTAAAACAACCACTGG+AGG - chr1.2:70773178-70773197 MS.gene60686:intron 45.0%
GACACTAAAACAACCACTGG+TGG - chr1.2:70773178-70773197 MS.gene60686:intron 45.0%
GGAAACAAAGGAGGAAGAAG+AGG - chr1.2:70774555-70774574 MS.gene60686:CDS 45.0%
TACAATGATGACCAGCCATC+TGG - chr1.2:70772845-70772864 MS.gene60686:CDS 45.0%
TCTGACACCAAAACAACCAC+CGG - chr1.2:70773076-70773095 MS.gene60686:intron 45.0%
! CAAGATGCTCATGTTTGCTG+TGG + chr1.2:70773360-70773379 None:intergenic 45.0%
! CATGCTTCTTGTGATGGAAG+AGG + chr1.2:70772721-70772740 None:intergenic 45.0%
! TATGGTGCCAAAACAACCAC+TGG - chr1.2:70772977-70772996 MS.gene60686:intron 45.0%
! TCTGGTGGTGACTATGAAAC+TGG - chr1.2:70772905-70772924 MS.gene60686:intron 45.0%
! TGCACCAACCACTTTGTTGT+TGG - chr1.2:70774040-70774059 MS.gene60686:CDS 45.0%
! TTGGACCGTTCAGATTTGCT+TGG - chr1.2:70774265-70774284 MS.gene60686:CDS 45.0%
!! GAAAAATATATAAAAAATTA+AGG + chr1.2:70773786-70773805 None:intergenic 5.0%
!!! TATTTTAAAATTTTCAATTT+TGG + chr1.2:70774365-70774384 None:intergenic 5.0%
AATGATGACCAGCCATCTGG+TGG - chr1.2:70772848-70772867 MS.gene60686:CDS 50.0%
ATCGTCACCATAACCACCAC+CGG + chr1.2:70773299-70773318 None:intergenic 50.0%
GACTCTGACACCAAAACCAC+TGG - chr1.2:70773025-70773044 MS.gene60686:intron 50.0%
GAGGAAGAAGAGGAAGCTCA+TGG - chr1.2:70774565-70774584 MS.gene60686:CDS 50.0%
TCTGACACCAAAACCACCAC+CGG - chr1.2:70773277-70773296 MS.gene60686:intron 50.0%
TCTGACACCAAAACCACTGG+TGG - chr1.2:70773028-70773047 MS.gene60686:intron 50.0%
TGTAACTACCACCAGATGGC+TGG + chr1.2:70772859-70772878 None:intergenic 50.0%
! TCTTGTGATGGAAGAGGCCA+CGG + chr1.2:70772715-70772734 None:intergenic 50.0%
!! AAAACCACTGGTGGTGGCTA+CGG - chr1.2:70773037-70773056 MS.gene60686:intron 50.0%
AAGACCCAGAGAACGCTCAC+AGG - chr1.2:70774452-70774471 MS.gene60686:CDS 55.0%
ACCAAAACAACCACCGGTGG+CGG - chr1.2:70773082-70773101 MS.gene60686:intron 55.0%
AGTTGCAGAAGCAGCTGCAG+TGG - chr1.2:70774492-70774511 MS.gene60686:CDS 55.0%
GACACCAAAACAACCACCGG+TGG - chr1.2:70773079-70773098 MS.gene60686:intron 55.0%
GACACCAAAACCACTGGTGG+TGG - chr1.2:70773031-70773050 MS.gene60686:intron 55.0%
GATGAAGAGAACCGTAGCCG+TGG - chr1.2:70772695-70772714 MS.gene60686:CDS 55.0%
GGTGCCAAAACAACCACTGG+CGG - chr1.2:70772980-70772999 MS.gene60686:intron 55.0%
GTTGCAGAAGCAGCTGCAGT+GGG - chr1.2:70774493-70774512 MS.gene60686:CDS 55.0%
TGTGCCTGTGAGCGTTCTCT+GGG + chr1.2:70774459-70774478 None:intergenic 55.0%
TTGTGCCTGTGAGCGTTCTC+TGG + chr1.2:70774460-70774479 None:intergenic 55.0%
! GTAGCCACCACCAGTGGTTT+TGG + chr1.2:70773038-70773057 None:intergenic 55.0%
!! GGCGGATATGGTGGTGGTTA+TGG - chr1.2:70773100-70773119 MS.gene60686:intron 55.0%
AATACCACTGGCGGCTACGG+TGG - chr1.2:70773139-70773158 MS.gene60686:intron 60.0%
ACAACCACTGGAGGCGGCTA+CGG - chr1.2:70773238-70773257 MS.gene60686:intron 60.0%
ACAACCACTGGCGGTGGCTA+CGG - chr1.2:70772989-70773008 MS.gene60686:intron 60.0%
ACCAAAACCACCACCGGTGG+TGG - chr1.2:70773283-70773302 MS.gene60686:intron 60.0%
ACCACCACCATATCCGCCAC+CGG + chr1.2:70773098-70773117 None:intergenic 60.0%
ACCATAACCACCACCGGTGG+TGG + chr1.2:70773293-70773312 None:intergenic 60.0%
CAGCTGCAGTGGGATCTGGT+GGG - chr1.2:70774503-70774522 MS.gene60686:CDS 60.0%
GAAGCAGCTGCAGTGGGATC+TGG - chr1.2:70774499-70774518 MS.gene60686:CDS 60.0%
GACACCAAAACCACCACCGG+TGG - chr1.2:70773280-70773299 MS.gene60686:intron 60.0%
GATGGAAGAGGCCACGGCTA+CGG + chr1.2:70772709-70772728 None:intergenic 60.0%
GCCAAAACAACCACTGGCGG+TGG - chr1.2:70772983-70773002 MS.gene60686:intron 60.0%
GGCGGCTACAGTGGTGGTTA+TGG - chr1.2:70773199-70773218 MS.gene60686:intron 60.0%
GTCACCATAACCACCACCGG+TGG + chr1.2:70773296-70773315 None:intergenic 60.0%
! ACCACCACCGGTGGTGGTTA+TGG - chr1.2:70773289-70773308 MS.gene60686:intron 60.0%
! AGCTGCCGGCGGTTTTGCTT+TGG - chr1.2:70773396-70773415 MS.gene60686:intron 60.0%
! GAGTTAGGTGCTGCAGCTGC+CGG - chr1.2:70773382-70773401 MS.gene60686:intron 60.0%
! GCCACCGCCAGTGGTTGTTT+TGG + chr1.2:70772987-70773006 None:intergenic 60.0%
! GGTGGCTACGGTGGTGGTTA+TGG - chr1.2:70773001-70773020 MS.gene60686:intron 60.0%
! TCCGCCACCGGTGGTTGTTT+TGG + chr1.2:70773086-70773105 None:intergenic 60.0%
!! ACAACCACCGGTGGCGGATA+TGG - chr1.2:70773088-70773107 MS.gene60686:intron 60.0%
!! ACCACCACCGGTGGTGGTTT+TGG + chr1.2:70773287-70773306 None:intergenic 60.0%
ACCACCATATCCGCCACCGG+TGG + chr1.2:70773095-70773114 None:intergenic 65.0%
ACCACCGTAGCCACCACCAG+TGG + chr1.2:70773044-70773063 None:intergenic 65.0%
ACCACTGTAGCCGCCACCAG+TGG + chr1.2:70773194-70773213 None:intergenic 65.0%
GCAGCTGCAGTGGGATCTGG+TGG - chr1.2:70774502-70774521 MS.gene60686:CDS 65.0%
! GGCGGCTACGGTGGTGGTTA+TGG - chr1.2:70773148-70773167 MS.gene60686:intron 65.0%
! TTAGGTGCTGCAGCTGCCGG+CGG - chr1.2:70773385-70773404 MS.gene60686:intron 65.0%
!! ACCACCGGTGGCGGATATGG+TGG - chr1.2:70773091-70773110 MS.gene60686:intron 65.0%
!! ACCACTGGTGGCGGCTACAG+TGG - chr1.2:70773190-70773209 MS.gene60686:intron 65.0%
!! ACCACTGGTGGTGGCTACGG+TGG - chr1.2:70773040-70773059 MS.gene60686:intron 65.0%
!! ACCGGTGGCGGATATGGTGG+TGG - chr1.2:70773094-70773113 MS.gene60686:intron 65.0%
!! ACTGGTGGCGGCTACAGTGG+TGG - chr1.2:70773193-70773212 MS.gene60686:intron 65.0%
!! ACTGGTGGTGGCTACGGTGG+TGG - chr1.2:70773043-70773062 MS.gene60686:intron 65.0%
!! GGCGGCTACGGTGGTGGATA+TGG - chr1.2:70773250-70773269 MS.gene60686:intron 65.0%
ACCACCACCGTAGCCGCCAG+TGG + chr1.2:70773146-70773165 None:intergenic 70.0%
ACCACCGTAGCCACCGCCAG+TGG + chr1.2:70772996-70773015 None:intergenic 70.0%
ACCACCGTAGCCGCCTCCAG+TGG + chr1.2:70773245-70773264 None:intergenic 70.0%
ACCACTGGAGGCGGCTACGG+TGG - chr1.2:70773241-70773260 MS.gene60686:intron 70.0%
ACCACTGGCGGCTACGGTGG+TGG - chr1.2:70773142-70773161 MS.gene60686:intron 70.0%
ACCACTGGCGGTGGCTACGG+TGG - chr1.2:70772992-70773011 MS.gene60686:intron 70.0%
ACTGGAGGCGGCTACGGTGG+TGG - chr1.2:70773244-70773263 MS.gene60686:intron 70.0%
ACTGGCGGTGGCTACGGTGG+TGG - chr1.2:70772995-70773014 MS.gene60686:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 70772689 70774615 70772689 ID=MS.gene60686
chr1.2 mRNA 70772689 70774615 70772689 ID=MS.gene60686.t1;Parent=MS.gene60686
chr1.2 exon 70773887 70774615 70773887 ID=MS.gene60686.t1.exon1;Parent=MS.gene60686.t1
chr1.2 CDS 70773887 70774615 70773887 ID=cds.MS.gene60686.t1;Parent=MS.gene60686.t1
chr1.2 exon 70772689 70772874 70772689 ID=MS.gene60686.t1.exon2;Parent=MS.gene60686.t1
chr1.2 CDS 70772689 70772874 70772689 ID=cds.MS.gene60686.t1;Parent=MS.gene60686.t1
Gene Sequence

>MS.gene60686

ATGTCTGATGAAGAGAACCGTAGCCGTGGCCTCTTCCATCACAAGAAGCATGATGATGATGATAACAGACCAATAGACACTGACAGTGGTTACAACAAAACATCATCATACTCTACAGGTGATGACTATGATTCTGGTTATAACAAAAAAACATCTTACAATGATGACCAGCCATCTGGTGGTAGTTACAATAAAACATCATATGGTAATGACAATTCTGGTGGTGACTATGAAACTGGTTACAATAAGAACACTGATAATTACTCTAATAATGAAACATCTGGTGGCTATGGTGCCAAAACAACCACTGGCGGTGGCTACGGTGGTGGTTATGGTGACTCTGACACCAAAACCACTGGTGGTGGCTACGGTGGTGGTTATGGTGACTCTGACACCAAAACAACCACCGGTGGCGGATATGGTGGTGGTTATGGTGACTCTGACACTAAAAATACCACTGGCGGCTACGGTGGTGGTTATGGTGACTCTGACACTAAAACAACCACTGGTGGCGGCTACAGTGGTGGTTATGGTGACTCTGACACTAAAACAACCACTGGAGGCGGCTACGGTGGTGGATATGGTGACTCTGACACCAAAACCACCACCGGTGGTGGTTATGGTGACGATCGTCGTGAAGACGTTGATTATAAGAACGAGGAGAAACGCCACAGCAAACATGAGCATCTTGGTGAGTTAGGTGCTGCAGCTGCCGGCGGTTTTGCTTTGGTAATTTTTTTTAATTACCATTACTCCTAATTGCTATTTAATCACTCCCATATTTTTGTTATTTGTTTCTTTTCTCTTTCGTTCTTTTTTATTTTGTTTTAATCAATAAATTTTCTCTGATGGTTAAAAATTTGCTATTTAATTTCTCCTTACAAAACATTCGAATAATTAATATTTGAATATTTCTATTTTTACAAATGAGAAAGAGAAATTATTATTAATTAAATAGATTTGTACAAAGTCTTTGTTGTTACAAGATCAAAATTCCAGTACCACTAAACTAACATCTAGTGAACTAAATAACTACTCTAATTTTTGAGAGTTCTGTACTAGAAAAACAATTTTACAAATTTATTTTTAATTTTCCTTAATTTTTTATATATTTTTCTTTAATAGAGACACATGCATGATTGAATGACTAAAAACTTGTCGTAGAATATTCAGTTTTACACAATCGCATAACCTAATCTTGTTGAGCATGACTCATATTCATTAATAAGTGGTCATAACCTTGAGTCGTTCCACGATGTTTGAGATAATCATTAGTAAATTCCAATCTGAACTGTAGTATTTAAACATTGCATTGTGCTATGTCGCCCTAAGATTTCCATAATAGGAAAATTGCACCAACCACTTTGTTGTTGGAGATTACACACAATCAGCCAAACTTTGTAATTAATTTTTTTAGTTCTTGTGTATTTTATTTACATGTTTTTTTGTAGAGTCAGGTATGATGCTCTAAGCCTATAACAAATACGAACGGTGAGATTAAGATCGGACAATTTACATTTAATTATTGTGAATTAAATCCAAAATAAAAAATTGAAATGTAAAAATTCGATCTTGATTGGACCGTTCAGATTTGCTTGGATGTTGGTAGGACAAACAATGTTGTATGTTACGCTATAAATAGACATAGGGTTTTCAGTATTCATCTTTTGAAACCAAAATTGAAAATTTTAAAATAATTGATTTTATGATTTTTTTTTTTTTTAAAATTTGTTTGATATAGTATGAGAAGCAAAAGTCAGAGAAAGACCCAGAGAACGCTCACAGGCACAAGATAGAAGAAGAAGTTGCAGAAGCAGCTGCAGTGGGATCTGGTGGGTTCGCCTTCCATGAGCATCATGAGAAAAAGGAAACAAAGGAGGAAGAAGAGGAAGCTCATGGAAAGAAGAAACACCATTTCTTTGGATGA
Protein sequence

>MS.gene60686.t1

MSDEENRSRGLFHHKKHDDDDNRPIDTDSGYNKTSSYSTGDDYDSGYNKKTSYNDDQPSGGSYNKTSYGNDNSGGDYETGYNKNTDNYSNNETSGGYGAKTTTGGGYGGGYGDSDTKTTGGGYGGGYGDSDTKTTTGGGYGGGYGDSDTKNTTGGYGGGYGDSDTKTTTGGGYSGGYGDSDTKTTTGGGYGGGYGDSDTKTTTGGGYGDDRREDVDYKNEEKRHSKHEHLGELGAAAAGGFALYEKQKSEKDPENAHRHKIEEEVAEAAAVGSGGFAFHEHHEKKETKEEEEEAHGKKKHHFFG