AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60735

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60735.t1 MTR_1g097690 91.212 660 9 4 1 659 1 612 0.0 1213
MS.gene60735.t1 MTR_1g097690 89.319 543 9 4 1 542 1 495 0.0 966
MS.gene60735.t1 MTR_6g042240 64.067 654 201 5 7 655 2 626 0.0 791
MS.gene60735.t1 MTR_6g042240 64.506 648 196 5 13 655 21 639 0.0 789
MS.gene60735.t1 MTR_6g042240 64.833 600 178 4 61 655 15 586 0.0 768
MS.gene60735.t1 MTR_5g014090 40.602 665 326 17 7 648 2 620 3.57e-145 436
MS.gene60735.t1 MTR_4g098850 38.602 658 277 13 11 643 283 838 5.55e-140 430
MS.gene60735.t1 MTR_4g098850 50.481 208 100 2 442 646 2 209 5.04e-66 233
MS.gene60735.t1 MTR_5g014090 36.429 560 282 15 12 547 7 516 2.69e-96 306
MS.gene60735.t1 MTR_1g071680 52.015 273 125 3 382 648 272 544 3.23e-93 299
MS.gene60735.t1 MTR_1g071680 32.086 187 106 4 12 191 7 179 4.76e-20 94.7
MS.gene60735.t1 MTR_5g006820 55.385 260 109 4 395 648 351 609 3.69e-93 301
MS.gene60735.t1 MTR_5g006820 33.908 174 102 2 22 191 17 181 2.33e-19 92.8
MS.gene60735.t1 MTR_7g095260 51.908 262 120 3 393 648 231 492 3.95e-91 292
MS.gene60735.t1 MTR_7g095260 40.708 113 67 0 79 191 15 127 6.73e-19 90.9
MS.gene60735.t1 MTR_7g095260 52.510 259 117 3 396 648 286 544 1.22e-90 292
MS.gene60735.t1 MTR_7g095260 35.632 174 104 2 18 191 14 179 5.17e-22 100
MS.gene60735.t1 MTR_1g103330 52.256 266 120 4 396 655 313 577 3.91e-90 292
MS.gene60735.t1 MTR_1g103330 32.584 178 105 3 18 191 13 179 6.85e-20 94.4
MS.gene60735.t1 MTR_8g043480 55.085 236 99 3 398 626 45 280 1.01e-86 274
MS.gene60735.t1 MTR_8g043480 55.085 236 99 3 398 626 175 410 2.71e-85 275
MS.gene60735.t1 MTR_8g043480 45.833 72 39 0 102 173 8 79 4.53e-15 78.6
MS.gene60735.t1 MTR_8g043480 55.085 236 99 3 398 626 240 475 5.05e-85 276
MS.gene60735.t1 MTR_8g043480 41.111 90 53 0 84 173 55 144 5.31e-16 81.6
MS.gene60735.t1 MTR_7g100070 52.263 243 104 5 401 639 175 409 4.05e-81 264
MS.gene60735.t1 MTR_7g100070 34.078 179 92 5 18 186 11 173 1.48e-18 89.4
MS.gene60735.t1 MTR_7g100070 52.263 243 104 5 401 639 174 408 4.11e-81 264
MS.gene60735.t1 MTR_7g100070 47.826 92 47 1 96 186 81 172 1.93e-18 89.0
MS.gene60735.t1 MTR_7g100070 52.263 243 104 5 401 639 215 449 3.56e-80 263
MS.gene60735.t1 MTR_7g100070 34.078 179 92 5 18 186 51 213 1.19e-18 90.1
MS.gene60735.t1 MTR_1g038810 53.017 232 103 2 401 626 176 407 6.98e-78 255
MS.gene60735.t1 MTR_1g038810 38.136 118 66 3 58 174 20 131 8.83e-13 71.2
MS.gene60735.t1 MTR_8g091360 48.846 260 126 4 395 647 312 571 9.01e-78 259
MS.gene60735.t1 MTR_8g091360 54.167 72 33 0 102 173 139 210 2.80e-17 86.3
MS.gene60735.t1 MTR_1g038810 53.017 232 103 2 401 626 180 411 1.00e-77 255
MS.gene60735.t1 MTR_1g038810 38.525 122 64 4 58 174 20 135 5.96e-14 75.1
MS.gene60735.t1 MTR_6g072010 50.000 230 111 2 401 626 155 384 1.57e-77 253
MS.gene60735.t1 MTR_6g072010 45.161 93 48 2 105 196 47 137 5.62e-17 84.0
MS.gene60735.t1 MTR_6g072010 50.000 230 111 2 401 626 166 395 2.96e-77 252
MS.gene60735.t1 MTR_6g072010 45.161 93 48 2 105 196 58 148 6.85e-17 83.6
MS.gene60735.t1 MTR_6g009260 49.174 242 113 6 395 626 160 401 1.02e-67 228
MS.gene60735.t1 MTR_6g009260 49.351 77 37 1 99 173 51 127 2.48e-15 79.3
MS.gene60735.t1 MTR_8g091360 49.007 151 74 1 395 542 312 462 2.94e-46 171
MS.gene60735.t1 MTR_8g091360 54.167 72 33 0 102 173 139 210 2.36e-17 85.9
MS.gene60735.t1 MTR_6g092670 32.589 224 121 5 397 592 642 863 5.43e-33 136
MS.gene60735.t1 MTR_1g078100 31.982 222 122 6 396 592 326 543 3.75e-31 130
MS.gene60735.t1 MTR_1g078100 31.982 222 122 6 396 592 606 823 3.91e-31 130
MS.gene60735.t1 MTR_1g078100 31.982 222 122 6 396 592 337 554 4.27e-31 130
MS.gene60735.t1 MTR_1g021730 31.429 245 145 12 386 618 75 308 5.63e-19 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60735.t1 AT1G71710 55.294 680 239 14 7 650 11 661 0.0 689
MS.gene60735.t1 AT1G71710 55.294 680 239 14 7 650 2 652 0.0 689
MS.gene60735.t1 AT1G34120 48.758 644 228 11 15 644 27 582 0.0 556
MS.gene60735.t1 AT1G34120 49.843 636 237 12 15 644 27 586 0.0 555
MS.gene60735.t1 AT1G34120 49.686 636 237 13 15 644 27 585 0.0 549
MS.gene60735.t1 AT1G34120 50.710 493 174 9 157 644 3 431 1.00e-153 451
MS.gene60735.t1 AT1G34120 45.438 537 192 10 15 538 27 475 3.63e-134 402
MS.gene60735.t1 AT4G18010 52.963 270 124 2 386 652 377 646 1.50e-96 310
MS.gene60735.t1 AT3G63240 53.409 264 117 3 391 648 271 534 6.52e-94 301
MS.gene60735.t1 AT3G63240 36.747 166 86 4 12 173 7 157 2.12e-19 92.8
MS.gene60735.t1 AT3G63240 53.409 264 117 3 391 648 271 534 6.52e-94 301
MS.gene60735.t1 AT3G63240 36.747 166 86 4 12 173 7 157 2.12e-19 92.8
MS.gene60735.t1 AT2G32010 55.598 259 108 4 396 648 324 581 1.09e-93 301
MS.gene60735.t1 AT2G32010 42.857 105 59 1 88 191 74 178 2.68e-19 92.4
MS.gene60735.t1 AT2G32010 55.598 259 108 4 396 648 324 581 1.09e-93 301
MS.gene60735.t1 AT2G32010 42.857 105 59 1 88 191 74 178 2.68e-19 92.4
MS.gene60735.t1 AT2G32010 55.598 259 108 4 396 648 324 581 1.09e-93 301
MS.gene60735.t1 AT2G32010 42.857 105 59 1 88 191 74 178 2.68e-19 92.4
MS.gene60735.t1 AT2G32010 55.598 259 108 4 396 648 324 581 1.09e-93 301
MS.gene60735.t1 AT2G32010 42.857 105 59 1 88 191 74 178 2.68e-19 92.4
MS.gene60735.t1 AT1G05470 53.668 259 113 4 396 648 347 604 2.40e-91 296
MS.gene60735.t1 AT1G05470 33.537 164 100 3 11 173 6 161 8.12e-21 97.4
MS.gene60735.t1 AT1G05470 53.668 259 113 4 396 648 347 604 2.40e-91 296
MS.gene60735.t1 AT1G05470 33.537 164 100 3 11 173 6 161 8.12e-21 97.4
MS.gene60735.t1 AT5G65090 50.189 265 126 3 385 643 186 450 1.79e-83 271
MS.gene60735.t1 AT5G65090 49.315 73 37 0 102 174 8 80 2.67e-17 85.5
MS.gene60735.t1 AT5G65090 50.189 265 126 3 385 643 249 513 7.88e-83 271
MS.gene60735.t1 AT5G65090 49.315 73 37 0 102 174 71 143 3.69e-17 85.5
MS.gene60735.t1 AT5G65090 50.189 265 126 3 385 643 249 513 7.88e-83 271
MS.gene60735.t1 AT5G65090 49.315 73 37 0 102 174 71 143 3.69e-17 85.5
MS.gene60735.t1 AT2G37440 54.237 236 102 4 397 628 112 345 1.15e-80 261
MS.gene60735.t1 AT2G37440 42.857 112 36 3 108 196 3 109 3.62e-14 75.5
MS.gene60735.t1 AT5G04980 48.828 256 124 3 386 634 145 400 1.28e-80 261
MS.gene60735.t1 AT2G37440 54.237 236 102 4 397 628 193 426 3.86e-80 262
MS.gene60735.t1 AT2G37440 48.571 105 51 2 100 201 79 183 6.72e-19 90.5
MS.gene60735.t1 AT2G37440 54.237 236 102 4 397 628 193 426 3.86e-80 262
MS.gene60735.t1 AT2G37440 48.571 105 51 2 100 201 79 183 6.72e-19 90.5
MS.gene60735.t1 AT5G04980 48.828 256 124 3 386 634 180 435 5.89e-80 261
MS.gene60735.t1 AT5G04980 47.826 69 36 0 102 170 7 75 4.32e-14 75.5
MS.gene60735.t1 AT5G04980 48.828 256 124 3 386 634 180 435 5.89e-80 261
MS.gene60735.t1 AT5G04980 47.826 69 36 0 102 170 7 75 4.32e-14 75.5
MS.gene60735.t1 AT5G04980 48.828 256 124 3 386 634 209 464 9.00e-80 261
MS.gene60735.t1 AT5G04980 47.826 69 36 0 102 170 36 104 4.06e-14 75.9
MS.gene60735.t1 AT2G01900 51.220 246 112 3 401 640 155 398 1.03e-76 251
MS.gene60735.t1 AT2G01900 37.500 120 67 3 69 187 29 141 1.41e-14 76.6
MS.gene60735.t1 AT2G01900 51.220 246 112 3 401 640 163 406 1.42e-76 251
MS.gene60735.t1 AT2G01900 37.500 120 67 3 69 187 37 149 1.41e-14 77.0
MS.gene60735.t1 AT2G01900 50.204 245 107 3 401 640 155 389 4.30e-74 244
MS.gene60735.t1 AT2G01900 37.500 120 67 3 69 187 29 141 1.45e-14 76.6
MS.gene60735.t1 AT4G18010 53.659 205 92 2 386 587 377 581 1.28e-73 248
MS.gene60735.t1 AT1G65580 31.618 272 141 8 396 624 351 620 3.21e-35 143
MS.gene60735.t1 AT1G65580 31.618 272 141 8 396 624 617 886 3.85e-35 143
MS.gene60735.t1 AT2G31830 29.766 299 149 8 395 648 658 940 1.40e-33 138
MS.gene60735.t1 AT2G31830 28.763 299 152 7 395 648 658 940 1.43e-33 138
MS.gene60735.t1 AT2G31830 32.510 243 132 6 395 609 658 896 1.19e-32 135
MS.gene60735.t1 AT1G05630 31.416 226 125 5 395 592 649 872 2.62e-32 134
MS.gene60735.t1 AT2G43900 30.973 226 126 5 395 592 655 878 2.66e-32 134
MS.gene60735.t1 AT2G43900 30.973 226 126 5 395 592 655 878 2.67e-32 134
MS.gene60735.t1 AT1G05630 31.416 226 125 5 395 592 649 872 3.33e-32 134
MS.gene60735.t1 AT1G47510 36.190 210 109 9 418 618 70 263 8.26e-25 105
MS.gene60735.t1 AT1G47510 36.190 210 109 9 418 618 132 325 3.91e-24 104
MS.gene60735.t1 AT1G05630 46.067 89 46 2 506 592 818 906 4.92e-17 86.3
MS.gene60735.t1 AT1G47510 44.444 117 54 5 510 618 149 262 5.70e-16 79.0

Find 125 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTTGTTTATAGAATTTA+TGG 0.084607 1.2:-71347584 MS.gene60735:intron
GGCAAAAGTCATTGACTTTA+AGG 0.135732 1.2:-71346791 MS.gene60735:CDS
GGTGAAGAACATAATTTATT+TGG 0.142900 1.2:-71345935 MS.gene60735:CDS
TGAATTAATCTCGTACTTAT+AGG 0.195385 1.2:+71344049 None:intergenic
TCATTGTCTGAATCAGTTTC+AGG 0.208520 1.2:+71347462 None:intergenic
AAAATGTTACTCGTGTTTAA+AGG 0.232239 1.2:+71346166 None:intergenic
AACTGTTGGTGTTGGTGTTA+TGG 0.238835 1.2:-71345315 MS.gene60735:CDS
AGAATTTCTTCTTCTATATC+TGG 0.254254 1.2:+71346001 None:intergenic
GCAAATGGCTTAACATTAAA+AGG 0.260784 1.2:-71347545 MS.gene60735:CDS
AGTGCTCGCTGTAGCTTCTT+TGG 0.278921 1.2:+71343621 None:intergenic
ACTGTTGGTGTTGGTGTTAT+GGG 0.280728 1.2:-71345314 MS.gene60735:CDS
TCTGATGGTTTAAACTTTGA+TGG 0.282944 1.2:+71346031 None:intergenic
TTATTTGCAGAAGAATAATT+TGG 0.290513 1.2:-71344651 MS.gene60735:intron
AACAGATGCTATAGATTTAC+TGG 0.299403 1.2:-71345480 MS.gene60735:CDS
GGTGTACGCCTCGCAACCTT+TGG 0.301674 1.2:+71344005 None:intergenic
GGGGAAGCCGGTCACGGATT+AGG 0.306749 1.2:-71347171 MS.gene60735:CDS
CTCATGATCCAAGATACTTT+TGG 0.312592 1.2:+71344961 None:intergenic
CAGAATTTCTCCCAGGCTTA+AGG 0.327355 1.2:-71346815 MS.gene60735:CDS
ACCCGTGACTGCCACATATA+TGG 0.328230 1.2:-71343664 MS.gene60735:CDS
CTTATCAAAGAAGGTTGAAT+AGG 0.329509 1.2:-71345814 MS.gene60735:CDS
AAGCTATCTAGAATGATTAA+TGG 0.329819 1.2:-71345725 MS.gene60735:CDS
GGGAAGCCGGTCACGGATTA+GGG 0.338322 1.2:-71347170 MS.gene60735:CDS
GGTGAGGAGATTCATCCTTT+AGG 0.342847 1.2:-71345956 MS.gene60735:CDS
TGGACTTCCCAAAAGTATCT+TGG 0.350599 1.2:-71344969 MS.gene60735:CDS
GTTGGTGTTATGGGCTATAT+TGG 0.372990 1.2:-71345305 MS.gene60735:CDS
TTCAGTGCTTCATCTATGAT+TGG 0.386944 1.2:+71345899 None:intergenic
GAGTACCTGTTGTTGCTTGT+TGG 0.389738 1.2:+71347838 None:intergenic
CACAGCCAACAAGCAACAAC+AGG 0.389941 1.2:-71347843 MS.gene60735:intron
CAACAATGGTCATGCGCAAA+TGG 0.392807 1.2:-71347560 MS.gene60735:CDS
AGCACTGAATATTAGTTTGA+AGG 0.396504 1.2:-71345885 MS.gene60735:CDS
CCTGCTGATATCTATGTCCT+TGG 0.397019 1.2:-71346562 MS.gene60735:CDS
ATGTGGCAGTCACGGGTCTA+TGG 0.397224 1.2:+71343670 None:intergenic
GAAGAAGTCACGACAAGTTT+CGG 0.399752 1.2:-71343570 MS.gene60735:CDS
TTAGAAAGCGATAGTGATAT+TGG 0.406935 1.2:-71345977 MS.gene60735:CDS
CATTTCCATGTGATAGAATA+CGG 0.410527 1.2:+71343739 None:intergenic
ATGACTTTGATATTGATGAT+TGG 0.413325 1.2:-71346600 MS.gene60735:CDS
GTCTTATTAAACGATTTAAC+CGG 0.414616 1.2:+71345539 None:intergenic
TCACGACAAGTTTCGGCACT+TGG 0.416258 1.2:-71343563 MS.gene60735:CDS
AGCTATCTAGAATGATTAAT+GGG 0.418456 1.2:-71345724 MS.gene60735:CDS
CACATATATGGCAGAGGTTG+AGG 0.419355 1.2:-71343652 MS.gene60735:CDS
CGTATTCTATCACATGGAAA+TGG 0.425799 1.2:-71343738 MS.gene60735:CDS
ATCAGTTATCTTATCAAAGA+AGG 0.426818 1.2:-71345823 MS.gene60735:CDS
TCTTTGTTTATAGAATTTAT+GGG 0.429558 1.2:-71347583 MS.gene60735:intron
GTGTACGCCTCGCAACCTTT+GGG 0.433772 1.2:+71344006 None:intergenic
GTTAGTGGGACTGAAAGAAT+TGG 0.433816 1.2:-71345632 MS.gene60735:CDS
TTTGTTGCCTCAGAAAGTAT+TGG 0.436972 1.2:-71345582 MS.gene60735:CDS
TACTCGTCCTGTACCGAAAT+GGG 0.439941 1.2:-71346131 MS.gene60735:CDS
TGCAGAAGAATAATTTGGTT+CGG 0.440082 1.2:-71344646 MS.gene60735:intron
AACAAATGTAATGGTGGCTC+TGG 0.447550 1.2:+71345599 None:intergenic
TCATCAATATCAAAGTCATT+AGG 0.451910 1.2:+71346604 None:intergenic
TTTCTATTAGAGTATGTGTT+GGG 0.452675 1.2:-71346654 MS.gene60735:intron
CCACCTTACGGCAGGTGAAA+AGG 0.453682 1.2:-71345068 MS.gene60735:CDS
ATACTCGTCCTGTACCGAAA+TGG 0.454190 1.2:-71346132 MS.gene60735:CDS
AAATTATGTTCTTCACCTAA+AGG 0.459993 1.2:+71345941 None:intergenic
TGCCATATATGTGGCAGTCA+CGG 0.462589 1.2:+71343662 None:intergenic
ATCTTGAAGCTTTGATGAAG+AGG 0.470630 1.2:-71345448 MS.gene60735:CDS
AGCATTTCGTTTGATTTCAT+CGG 0.472148 1.2:+71345042 None:intergenic
CTTTATATGCACCCACCTTA+CGG 0.476641 1.2:-71345080 MS.gene60735:CDS
GGTTTAAACTTTGATGGAGA+TGG 0.477628 1.2:+71346037 None:intergenic
ATATCTGGTGCATCATCTGA+TGG 0.479222 1.2:+71346016 None:intergenic
GCCATATATGTGGCAGTCAC+GGG 0.485409 1.2:+71343663 None:intergenic
ATCTCGTACTTATAGGTTGG+TGG 0.485679 1.2:+71344056 None:intergenic
GATTAGTAAGCTATCTAGAA+TGG 0.486586 1.2:-71345654 MS.gene60735:CDS
TATCATATGGCAAGCTGATA+CGG 0.487049 1.2:+71344611 None:intergenic
TTAACCGGTTTAAAAGAAGT+TGG 0.498071 1.2:+71345554 None:intergenic
CTGAAAGAATTGGTTTGAGC+TGG 0.499133 1.2:-71345622 MS.gene60735:CDS
CTGCTGATATCTATGTCCTT+GGG 0.499146 1.2:-71346561 MS.gene60735:intron
AACATGAAGGTTTCAACTGT+TGG 0.500406 1.2:-71345329 MS.gene60735:CDS
CAGGAGAGAGGATGAAGCTC+CGG 0.509164 1.2:-71347146 MS.gene60735:CDS
TGAGATTACTGAGTTACAAA+AGG 0.512142 1.2:-71343713 MS.gene60735:CDS
TCCTGTCCCTAATCCGTGAC+CGG 0.513210 1.2:+71347164 None:intergenic
AAGGTTTCAACTGTTGGTGT+TGG 0.514208 1.2:-71345323 MS.gene60735:CDS
TTAATCTCGTACTTATAGGT+TGG 0.517794 1.2:+71344053 None:intergenic
TTCTGAGGCAACAAATGTAA+TGG 0.523939 1.2:+71345590 None:intergenic
TGAACTGCAGAATTTCTCCC+AGG 0.530982 1.2:-71346822 MS.gene60735:intron
CCAAGGACATAGATATCAGC+AGG 0.535441 1.2:+71346562 None:intergenic
GCCGGTCACGGATTAGGGAC+AGG 0.536950 1.2:-71347165 MS.gene60735:CDS
TTAGAGTATGTGTTGGGACA+TGG 0.539774 1.2:-71346648 MS.gene60735:intron
ATTAATTCAGACAAGTACAT+TGG 0.541947 1.2:-71344035 MS.gene60735:CDS
TGTTCTGAATATGTTTCCGC+AGG 0.543544 1.2:+71345348 None:intergenic
CAGAGTGGGGAAGCCGGTCA+CGG 0.545381 1.2:-71347177 MS.gene60735:CDS
TCATCACGGTATGGGTTCGA+AGG 0.552651 1.2:-71345373 MS.gene60735:CDS
CTTGTGAAAGAATTAGAGAA+AGG 0.553223 1.2:-71344116 MS.gene60735:CDS
TGTGTTGGGACATGGAATGT+TGG 0.555711 1.2:-71346640 MS.gene60735:CDS
TCTCGTACTTATAGGTTGGT+GGG 0.555840 1.2:+71344057 None:intergenic
TGGAGATGGAGGATCACTAA+AGG 0.558217 1.2:+71346051 None:intergenic
GAAACATATTCAGAACATGA+AGG 0.559314 1.2:-71345342 MS.gene60735:CDS
CTCAACCTCTGCCATATATG+TGG 0.563035 1.2:+71343653 None:intergenic
ATAGAATTTATGGGCAACAA+TGG 0.563404 1.2:-71347574 MS.gene60735:intron
AAGCTATCTAGAATGGTTAG+TGG 0.565033 1.2:-71345647 MS.gene60735:CDS
ATATGCACCCACCTTACGGC+AGG 0.566040 1.2:-71345076 MS.gene60735:CDS
GAAACTGATTCAGACAATGA+AGG 0.567354 1.2:-71347459 MS.gene60735:intron
ATGGCTTAACATTAAAAGGA+AGG 0.567804 1.2:-71347541 MS.gene60735:CDS
TTAAACTTTGATGGAGATGG+AGG 0.571133 1.2:+71346040 None:intergenic
TAGTCACCATCTGATTCAGC+CGG 0.571583 1.2:+71347127 None:intergenic
AGCTATCTAGAATGGTTAGT+GGG 0.573263 1.2:-71345646 MS.gene60735:CDS
GGTCTCTCCAATACTTTCTG+AGG 0.576164 1.2:+71345575 None:intergenic
TTTCCGCGAGGAAGATTCAT+CGG 0.585056 1.2:-71345783 MS.gene60735:CDS
ATTGTGCAGAGTGGGGAAGC+CGG 0.586219 1.2:-71347183 MS.gene60735:intron
TCAATATCAAAGTCATTAGG+TGG 0.586310 1.2:+71346607 None:intergenic
GACTGCCACATATATGGCAG+AGG 0.590077 1.2:-71343658 MS.gene60735:CDS
GTCTCAATATATAAACAAGA+AGG 0.590829 1.2:-71346769 MS.gene60735:CDS
ATTTCAGACAGTGATGAAGC+AGG 0.595377 1.2:-71347879 None:intergenic
GAAGCTCCGGCTGAATCAGA+TGG 0.599862 1.2:-71347133 MS.gene60735:intron
TGCGACCGTATTCTATCACA+TGG 0.604470 1.2:-71343744 MS.gene60735:CDS
CAAACTAAAGCATACCATGC+TGG 0.608342 1.2:+71343984 None:intergenic
ATAGTGAGCAAGCAGATGGT+TGG 0.609242 1.2:-71345401 MS.gene60735:CDS
GTTGGGACATGGAATGTTGG+AGG 0.610467 1.2:-71346637 MS.gene60735:CDS
AAGCGATAGTGATATTGGTG+AGG 0.614723 1.2:-71345972 MS.gene60735:CDS
AATTTCTCCCAGGCTTAAGG+AGG 0.618284 1.2:-71346812 MS.gene60735:CDS
ACGGATTAGGGACAGGAGAG+AGG 0.618825 1.2:-71347158 MS.gene60735:CDS
TATGGGCTATATTGGTAACA+AGG 0.624421 1.2:-71345297 MS.gene60735:intron
TTCAGACAAGTACATTGGAG+AGG 0.633311 1.2:-71344030 MS.gene60735:CDS
GAGAGGACCCAAAGGTTGCG+AGG 0.634911 1.2:-71344013 MS.gene60735:CDS
TGAGGCAACAAATGTAATGG+TGG 0.637973 1.2:+71345593 None:intergenic
GGACATAGATATCAGCAGGA+TGG 0.644979 1.2:+71346566 None:intergenic
TTGCGAGGCGTACACCAGCA+TGG 0.653553 1.2:-71343998 MS.gene60735:intron
TATGGGTTCGAAGGAGCCTG+CGG 0.674551 1.2:-71345364 MS.gene60735:CDS
GTACATTGGAGAGGACCCAA+AGG 0.674845 1.2:-71344021 MS.gene60735:CDS
GAAGATAGTGAGCAAGCAGA+TGG 0.678985 1.2:-71345405 MS.gene60735:CDS
ATGTTCGTGAAATACATCAG+AGG 0.682749 1.2:-71345018 MS.gene60735:CDS
AAAATTGGTTGAGAGAGACC+AGG 0.701785 1.2:-71344554 MS.gene60735:intron
ATATAAACAAGAAGGAACTG+AGG 0.702635 1.2:-71346761 MS.gene60735:intron
TTTCCGATGAATCTTCCTCG+CGG 0.752096 1.2:+71345780 None:intergenic
TAGGCTAAATCATTTCCGCG+AGG 0.776950 1.2:-71345795 MS.gene60735:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTAATTAATAAAACAT+TGG - chr1.2:71344000-71344019 MS.gene60735:CDS 10.0%
!! ATTTCTTTATAAATTCTAGT+CGG - chr1.2:71343694-71343713 MS.gene60735:CDS 15.0%
!! TAAATTAACATATTTGAATG+TGG + chr1.2:71346498-71346517 None:intergenic 15.0%
!! TATTAAAAGTGATTTCAAAA+TGG + chr1.2:71344930-71344949 None:intergenic 15.0%
!! TTCTAATTTAATCATATAGA+AGG + chr1.2:71346633-71346652 None:intergenic 15.0%
!! TTTATGAGTATGATAAAAAA+TGG + chr1.2:71347516-71347535 None:intergenic 15.0%
!!! CATTTTTCGATTTAAATTTA+GGG + chr1.2:71344479-71344498 None:intergenic 15.0%
!!! TCTTTGTTTATAGAATTTAT+GGG - chr1.2:71343831-71343850 MS.gene60735:intron 15.0%
!!! TTACCTTAATTTTAATCAAA+AGG + chr1.2:71346549-71346568 None:intergenic 15.0%
!! AACATAGATAATCGTATTTA+AGG - chr1.2:71347178-71347197 MS.gene60735:intron 20.0%
!! AATCAGAAAGTGAATAAAAA+TGG + chr1.2:71346424-71346443 None:intergenic 20.0%
!! ACATAGATAATCGTATTTAA+GGG - chr1.2:71347179-71347198 MS.gene60735:intron 20.0%
!! ATCAGAAAGTGAATAAAAAT+GGG + chr1.2:71346423-71346442 None:intergenic 20.0%
!! CCTAAATTTAAATCGAAAAA+TGG - chr1.2:71344477-71344496 MS.gene60735:intron 20.0%
!! GATTAATTTACTTCAATAGA+AGG + chr1.2:71346656-71346675 None:intergenic 20.0%
!! TCATGAAATCAAAATGAATA+AGG + chr1.2:71347229-71347248 None:intergenic 20.0%
!! TTATTTGCAGAAGAATAATT+TGG - chr1.2:71346763-71346782 MS.gene60735:CDS 20.0%
!!! AAAGTGTTTTTATATACACT+TGG - chr1.2:71345156-71345175 MS.gene60735:intron 20.0%
!!! ATGCCTTTTGATTAAAATTA+AGG - chr1.2:71346543-71346562 MS.gene60735:intron 20.0%
!!! CCATTTTTCGATTTAAATTT+AGG + chr1.2:71344480-71344499 None:intergenic 20.0%
!!! GTCTTTGTTTATAGAATTTA+TGG - chr1.2:71343830-71343849 MS.gene60735:intron 20.0%
!!! TTTTATTCACTTTCTGATTT+TGG - chr1.2:71346425-71346444 MS.gene60735:intron 20.0%
! AAAATGTTACTCGTGTTTAA+AGG + chr1.2:71345251-71345270 None:intergenic 25.0%
! AAATTATGTTCTTCACCTAA+AGG + chr1.2:71345476-71345495 None:intergenic 25.0%
! ACCTTATCTATAATCTATGT+GGG - chr1.2:71343717-71343736 MS.gene60735:CDS 25.0%
! ACGGAACATATATTTCTTAA+TGG + chr1.2:71347009-71347028 None:intergenic 25.0%
! AGAATTTCTTCTTCTATATC+TGG + chr1.2:71345416-71345435 None:intergenic 25.0%
! AGACTATTCATTTGAAGATT+TGG - chr1.2:71344054-71344073 MS.gene60735:CDS 25.0%
! ATAATCGTATTTAAGGGTTT+AGG - chr1.2:71347185-71347204 MS.gene60735:intron 25.0%
! ATCAGTTATCTTATCAAAGA+AGG - chr1.2:71345591-71345610 MS.gene60735:CDS 25.0%
! ATTAATTCAGACAAGTACAT+TGG - chr1.2:71347379-71347398 MS.gene60735:intron 25.0%
! ATTTGAAGATTTGGAAATTG+AGG - chr1.2:71344063-71344082 MS.gene60735:CDS 25.0%
! CGATTATCTATGTTACAAAA+TGG + chr1.2:71347172-71347191 None:intergenic 25.0%
! GGTGAAGAACATAATTTATT+TGG - chr1.2:71345479-71345498 MS.gene60735:CDS 25.0%
! GTCTCAATATATAAACAAGA+AGG - chr1.2:71344645-71344664 MS.gene60735:intron 25.0%
! TAATCGTATTTAAGGGTTTA+GGG - chr1.2:71347186-71347205 MS.gene60735:intron 25.0%
! TCATCAATATCAAAGTCATT+AGG + chr1.2:71344813-71344832 None:intergenic 25.0%
! TGAATTAATCTCGTACTTAT+AGG + chr1.2:71347368-71347387 None:intergenic 25.0%
! TTTCTATTAGAGTATGTGTT+GGG - chr1.2:71344760-71344779 MS.gene60735:intron 25.0%
!! AAGCTATCTAGAATGATTAA+TGG - chr1.2:71345689-71345708 MS.gene60735:CDS 25.0%
!! AGCTATCTAGAATGATTAAT+GGG - chr1.2:71345690-71345709 MS.gene60735:CDS 25.0%
!! ATCTTATCTCAAAAAAGCAT+TGG - chr1.2:71346835-71346854 MS.gene60735:intron 25.0%
!! ATGACTTTGATATTGATGAT+TGG - chr1.2:71344814-71344833 MS.gene60735:intron 25.0%
!! CTAAAGGATTTTATCTTTGA+AGG + chr1.2:71345350-71345369 None:intergenic 25.0%
!! GATTTTGAACTCAAGTTAAA+AGG - chr1.2:71347253-71347272 MS.gene60735:intron 25.0%
!! GTCTTATTAAACGATTTAAC+CGG + chr1.2:71345878-71345897 None:intergenic 25.0%
!! TGATCTGTCTCAATTTTATT+TGG - chr1.2:71344204-71344223 MS.gene60735:intron 25.0%
!! TTTTCTATTAGAGTATGTGT+TGG - chr1.2:71344759-71344778 MS.gene60735:intron 25.0%
!!! AAAAAAGCATTGGTCAAAAT+TGG - chr1.2:71346845-71346864 MS.gene60735:intron 25.0%
!!! AGATCTCTTGTTTTATCATA+TGG + chr1.2:71346819-71346838 None:intergenic 25.0%
!!! CTTATTTTTATACTGGATAC+CGG - chr1.2:71343622-71343641 MS.gene60735:CDS 25.0%
!!! GATCTGTCTCAATTTTATTT+GGG - chr1.2:71344205-71344224 MS.gene60735:intron 25.0%
!!! TATCAAGTTGATACATCTAA+TGG + chr1.2:71346898-71346917 None:intergenic 25.0%
!!! TTATTGATGTCTTATTTCTG+AGG - chr1.2:71346968-71346987 MS.gene60735:intron 25.0%
!!! TTTTGATTTGAAAGAATCAC+AGG + chr1.2:71345915-71345934 None:intergenic 25.0%
AACAGATGCTATAGATTTAC+TGG - chr1.2:71345934-71345953 MS.gene60735:CDS 30.0%
AATGTTTGCAATTACCTTAC+AGG - chr1.2:71347647-71347666 MS.gene60735:intron 30.0%
ACCAAAAACTACAAACTCTA+GGG + chr1.2:71346168-71346187 None:intergenic 30.0%
ACCAATGTCTTGAATTTACA+TGG - chr1.2:71346577-71346596 MS.gene60735:CDS 30.0%
AGGCATTAGCATATAATTCA+AGG + chr1.2:71346529-71346548 None:intergenic 30.0%
ATAGAATTTATGGGCAACAA+TGG - chr1.2:71343840-71343859 MS.gene60735:intron 30.0%
ATATAAACAAGAAGGAACTG+AGG - chr1.2:71344653-71344672 MS.gene60735:intron 30.0%
ATGGCTTAACATTAAAAGGA+AGG - chr1.2:71343873-71343892 MS.gene60735:intron 30.0%
CATTTCCATGTGATAGAATA+CGG + chr1.2:71347678-71347697 None:intergenic 30.0%
CTTATCAAAGAAGGTTGAAT+AGG - chr1.2:71345600-71345619 MS.gene60735:CDS 30.0%
CTTGTGAAAGAATTAGAGAA+AGG - chr1.2:71347298-71347317 MS.gene60735:intron 30.0%
GAAACATATTCAGAACATGA+AGG - chr1.2:71346072-71346091 MS.gene60735:CDS 30.0%
GACCTTATCTATAATCTATG+TGG - chr1.2:71343716-71343735 MS.gene60735:CDS 30.0%
GATTAGTAAGCTATCTAGAA+TGG - chr1.2:71345682-71345701 MS.gene60735:CDS 30.0%
GATTTGGAAATTGAGGATTA+TGG - chr1.2:71344070-71344089 MS.gene60735:CDS 30.0%
GCAAATGGCTTAACATTAAA+AGG - chr1.2:71343869-71343888 MS.gene60735:intron 30.0%
GTATAAAAATAAGCCATGCA+AGG + chr1.2:71343615-71343634 None:intergenic 30.0%
TCAATATCAAAGTCATTAGG+TGG + chr1.2:71344810-71344829 None:intergenic 30.0%
TCCCACATAGATTATAGATA+AGG + chr1.2:71343721-71343740 None:intergenic 30.0%
TCTGATGGTTTAAACTTTGA+TGG + chr1.2:71345386-71345405 None:intergenic 30.0%
TGAGATTACTGAGTTACAAA+AGG - chr1.2:71347701-71347720 MS.gene60735:intron 30.0%
TGCAGAAGAATAATTTGGTT+CGG - chr1.2:71346768-71346787 MS.gene60735:CDS 30.0%
TTAATCTCGTACTTATAGGT+TGG + chr1.2:71347364-71347383 None:intergenic 30.0%
TTAGAAAGCGATAGTGATAT+TGG - chr1.2:71345437-71345456 MS.gene60735:CDS 30.0%
TTGACAATTCTTCCTGTATT+AGG - chr1.2:71344433-71344452 MS.gene60735:intron 30.0%
! AACTCTAGGGAAAATTTCAA+AGG + chr1.2:71346155-71346174 None:intergenic 30.0%
! AGCACTGAATATTAGTTTGA+AGG - chr1.2:71345529-71345548 MS.gene60735:CDS 30.0%
! AGCATTTCGTTTGATTTCAT+CGG + chr1.2:71346375-71346394 None:intergenic 30.0%
! AGTTGATACATCTAATGGAA+GGG + chr1.2:71346893-71346912 None:intergenic 30.0%
! CGAATTAAGTTTTCCCATTT+CGG + chr1.2:71345299-71345318 None:intergenic 30.0%
! TCATGATCCAAGATACTTTT+GGG + chr1.2:71346455-71346474 None:intergenic 30.0%
! TTAACCGGTTTAAAAGAAGT+TGG + chr1.2:71345863-71345882 None:intergenic 30.0%
!! AAGTTGATACATCTAATGGA+AGG + chr1.2:71346894-71346913 None:intergenic 30.0%
!! GTGTTTTTATATACACTTGG+TGG - chr1.2:71345159-71345178 MS.gene60735:intron 30.0%
!!! TGCATGGCTTATTTTTATAC+TGG - chr1.2:71343615-71343634 MS.gene60735:CDS 30.0%
!!! TTAGAGAAAGGTGTTTTTGA+TGG - chr1.2:71347310-71347329 MS.gene60735:intron 30.0%
AACATGAAGGTTTCAACTGT+TGG - chr1.2:71346085-71346104 MS.gene60735:CDS 35.0%
AATTTCGAATGGCCTAATAC+AGG + chr1.2:71344448-71344467 None:intergenic 35.0%
ATAGTCTTCTGCTAATGACA+CGG + chr1.2:71344041-71344060 None:intergenic 35.0%
ATGTTCGTGAAATACATCAG+AGG - chr1.2:71346396-71346415 MS.gene60735:intron 35.0%
ATTAGGCCATTCGAAATTTG+TGG - chr1.2:71344450-71344469 MS.gene60735:intron 35.0%
CAACAGAATTCAGATATGAG+AGG + chr1.2:71344734-71344753 None:intergenic 35.0%
CATACTCATCCTTACAAAAC+CGG - chr1.2:71343652-71343671 MS.gene60735:CDS 35.0%
CATCATCATCATCATCATCA+GGG + chr1.2:71343921-71343940 None:intergenic 35.0%
CGTATTCTATCACATGGAAA+TGG - chr1.2:71347676-71347695 MS.gene60735:intron 35.0%
GACCAAAAACTACAAACTCT+AGG + chr1.2:71346169-71346188 None:intergenic 35.0%
GCCATGTAAATTCAAGACAT+TGG + chr1.2:71346581-71346600 None:intergenic 35.0%
GGTTTAAACTTTGATGGAGA+TGG + chr1.2:71345380-71345399 None:intergenic 35.0%
TATCATATGGCAAGCTGATA+CGG + chr1.2:71346806-71346825 None:intergenic 35.0%
TCATCATCATCATCATCATC+AGG + chr1.2:71343922-71343941 None:intergenic 35.0%
TCATTGTCTGAATCAGTTTC+AGG + chr1.2:71343955-71343974 None:intergenic 35.0%
TCGATGAATTTACTGAGTGA+CGG + chr1.2:71347028-71347047 None:intergenic 35.0%
TGCTGAAATTCTGTATTCAC+TGG - chr1.2:71346699-71346718 MS.gene60735:intron 35.0%
TGGGACTGAAAAAGATTCAT+CGG - chr1.2:71345709-71345728 MS.gene60735:CDS 35.0%
TGTAAGGATGAGTATGAGTT+AGG + chr1.2:71343648-71343667 None:intergenic 35.0%
TGTGTGATGAATGATGATTC+TGG - chr1.2:71344162-71344181 MS.gene60735:intron 35.0%
TTAAACTTTGATGGAGATGG+AGG + chr1.2:71345377-71345396 None:intergenic 35.0%
TTCAGTGCTTCATCTATGAT+TGG + chr1.2:71345518-71345537 None:intergenic 35.0%
TTGTGAGTATTTGGAAACTG+AGG - chr1.2:71343763-71343782 MS.gene60735:intron 35.0%
TTTAGTGTGAAACTTACCCA+AGG + chr1.2:71344872-71344891 None:intergenic 35.0%
! AAGCTATCTAGAATGGTTAG+TGG - chr1.2:71345767-71345786 MS.gene60735:CDS 35.0%
! AGCTATCTAGAATGGTTAGT+GGG - chr1.2:71345768-71345787 MS.gene60735:CDS 35.0%
! AGTTGGTGTTTGTGAGTATT+TGG - chr1.2:71343754-71343773 MS.gene60735:intron 35.0%
! ATCTTGAAGCTTTGATGAAG+AGG - chr1.2:71345966-71345985 MS.gene60735:CDS 35.0%
! CTCATGATCCAAGATACTTT+TGG + chr1.2:71346456-71346475 None:intergenic 35.0%
! GAAACTGATTCAGACAATGA+AGG - chr1.2:71343955-71343974 MS.gene60735:intron 35.0%
! GAGACCAACTTCTTTTAAAC+CGG - chr1.2:71345856-71345875 MS.gene60735:CDS 35.0%
! GAGCTGAAGTGTATATTTTC+TGG - chr1.2:71343803-71343822 MS.gene60735:intron 35.0%
! TAATGGAAGGGTATATTACC+TGG + chr1.2:71346881-71346900 None:intergenic 35.0%
! TATGGGCTATATTGGTAACA+AGG - chr1.2:71346117-71346136 MS.gene60735:CDS 35.0%
! TCCCTAGAGTTTGTAGTTTT+TGG - chr1.2:71346164-71346183 MS.gene60735:CDS 35.0%
! TGATGATGATGATGATGATG+AGG - chr1.2:71343921-71343940 MS.gene60735:intron 35.0%
! TGGTATTTTCATCACGGTAT+GGG - chr1.2:71346033-71346052 MS.gene60735:CDS 35.0%
! TTCATTTTCGATCTCTGCAT+CGG + chr1.2:71347827-71347846 None:intergenic 35.0%
! TTGGTATTTTCATCACGGTA+TGG - chr1.2:71346032-71346051 MS.gene60735:CDS 35.0%
! TTTGTTGCCTCAGAAAGTAT+TGG - chr1.2:71345832-71345851 MS.gene60735:CDS 35.0%
!! ATGATGATTCTGGTTCAGTT+TGG - chr1.2:71344172-71344191 MS.gene60735:intron 35.0%
!! GATGGTTGGTATTTTCATCA+CGG - chr1.2:71346027-71346046 MS.gene60735:CDS 35.0%
!! GGCAAAAGTCATTGACTTTA+AGG - chr1.2:71344623-71344642 MS.gene60735:CDS 35.0%
!! TTCTGAGGCAACAAATGTAA+TGG + chr1.2:71345827-71345846 None:intergenic 35.0%
!!! AGAAAGGTGTTTTTGATGGA+TGG - chr1.2:71347314-71347333 MS.gene60735:intron 35.0%
AAAATTGGTTGAGAGAGACC+AGG - chr1.2:71346860-71346879 MS.gene60735:intron 40.0%
AACAAATGTAATGGTGGCTC+TGG + chr1.2:71345818-71345837 None:intergenic 40.0%
AACAAGAAGGAACTGAGGTT+AGG - chr1.2:71344658-71344677 MS.gene60735:intron 40.0%
AAGCGATAGTGATATTGGTG+AGG - chr1.2:71345442-71345461 MS.gene60735:CDS 40.0%
AAGGTTTCAACTGTTGGTGT+TGG - chr1.2:71346091-71346110 MS.gene60735:CDS 40.0%
AATGATGTATCACCATCCGA+CGG + chr1.2:71344385-71344404 None:intergenic 40.0%
ATATCTGGTGCATCATCTGA+TGG + chr1.2:71345401-71345420 None:intergenic 40.0%
ATCATCATCATCATCAGGGT+CGG + chr1.2:71343917-71343936 None:intergenic 40.0%
CAAACTAAAGCATACCATGC+TGG + chr1.2:71347433-71347452 None:intergenic 40.0%
CAAGACTAGTATAATGCCGT+CGG - chr1.2:71344366-71344385 MS.gene60735:intron 40.0%
CATTCATCACACACAAGACA+TGG + chr1.2:71344155-71344174 None:intergenic 40.0%
CTGCTGATATCTATGTCCTT+GGG - chr1.2:71344853-71344872 MS.gene60735:intron 40.0%
CTTTATATGCACCCACCTTA+CGG - chr1.2:71346334-71346353 MS.gene60735:intron 40.0%
GAAGAAGTCACGACAAGTTT+CGG - chr1.2:71347844-71347863 MS.gene60735:CDS 40.0%
GCTCTCAAAAGTCGAATATG+AGG + chr1.2:71344505-71344524 None:intergenic 40.0%
GGGAATCCACAAATTTCGAA+TGG + chr1.2:71344459-71344478 None:intergenic 40.0%
GTTAGTGGGACTGAAAGAAT+TGG - chr1.2:71345782-71345801 MS.gene60735:CDS 40.0%
TGAGGCAACAAATGTAATGG+TGG + chr1.2:71345824-71345843 None:intergenic 40.0%
TTAGAGTATGTGTTGGGACA+TGG - chr1.2:71344766-71344785 MS.gene60735:intron 40.0%
TTCAGACAAGTACATTGGAG+AGG - chr1.2:71347384-71347403 MS.gene60735:intron 40.0%
TTTCTTTGTTCAGCCTTGCA+TGG - chr1.2:71343599-71343618 MS.gene60735:CDS 40.0%
! AAGTTTTCCCATTTCGGTAC+AGG + chr1.2:71345293-71345312 None:intergenic 40.0%
! ATCTCGTACTTATAGGTTGG+TGG + chr1.2:71347361-71347380 None:intergenic 40.0%
! TGTTCTGAATATGTTTCCGC+AGG + chr1.2:71346069-71346088 None:intergenic 40.0%
!! AACTGTTGGTGTTGGTGTTA+TGG - chr1.2:71346099-71346118 MS.gene60735:CDS 40.0%
!! ACTGTTGGTGTTGGTGTTAT+GGG - chr1.2:71346100-71346119 MS.gene60735:CDS 40.0%
!! CTGAAAGAATTGGTTTGAGC+TGG - chr1.2:71345792-71345811 MS.gene60735:CDS 40.0%
!! CTGCAAATTCTGTTTCTGCT+TGG - chr1.2:71346675-71346694 MS.gene60735:intron 40.0%
!! GTTGGTGTTATGGGCTATAT+TGG - chr1.2:71346109-71346128 MS.gene60735:CDS 40.0%
!! GTTTTTGATGGATGGTCAGA+AGG - chr1.2:71347322-71347341 MS.gene60735:intron 40.0%
!! TCTCGTACTTATAGGTTGGT+GGG + chr1.2:71347360-71347379 None:intergenic 40.0%
!! TCTTACAAGCCGGTTTTGTA+AGG + chr1.2:71343664-71343683 None:intergenic 40.0%
!! TGGACTTCCCAAAAGTATCT+TGG - chr1.2:71346445-71346464 MS.gene60735:intron 40.0%
!! TTACAAAACCGGCTTGTAAG+AGG - chr1.2:71343663-71343682 MS.gene60735:CDS 40.0%
AATTTCTCCCAGGCTTAAGG+AGG - chr1.2:71344602-71344621 MS.gene60735:CDS 45.0%
ACACTCATCCTCTTACAAGC+CGG + chr1.2:71343674-71343693 None:intergenic 45.0%
ACTAGTATAATGCCGTCGGA+TGG - chr1.2:71344370-71344389 MS.gene60735:intron 45.0%
AGGATGAGTATGAGTTAGGC+CGG + chr1.2:71343644-71343663 None:intergenic 45.0%
ATACTCGTCCTGTACCGAAA+TGG - chr1.2:71345282-71345301 MS.gene60735:intron 45.0%
ATAGTGAGCAAGCAGATGGT+TGG - chr1.2:71346013-71346032 MS.gene60735:CDS 45.0%
ATGAATTGTGCTCCAGAGGA+AGG + chr1.2:71346944-71346963 None:intergenic 45.0%
CAACAATGGTCATGCGCAAA+TGG - chr1.2:71343854-71343873 MS.gene60735:intron 45.0%
CACATATATGGCAGAGGTTG+AGG - chr1.2:71347762-71347781 MS.gene60735:intron 45.0%
CAGAATTTCTCCCAGGCTTA+AGG - chr1.2:71344599-71344618 MS.gene60735:CDS 45.0%
CCAAGGACATAGATATCAGC+AGG + chr1.2:71344855-71344874 None:intergenic 45.0%
CCTGCTGATATCTATGTCCT+TGG - chr1.2:71344852-71344871 MS.gene60735:intron 45.0%
CTCAACCTCTGCCATATATG+TGG + chr1.2:71347764-71347783 None:intergenic 45.0%
GAAGATAGTGAGCAAGCAGA+TGG - chr1.2:71346009-71346028 MS.gene60735:CDS 45.0%
GGACATAGATATCAGCAGGA+TGG + chr1.2:71344851-71344870 None:intergenic 45.0%
GGTCTCTCCAATACTTTCTG+AGG + chr1.2:71345842-71345861 None:intergenic 45.0%
GGTGAGGAGATTCATCCTTT+AGG - chr1.2:71345458-71345477 MS.gene60735:CDS 45.0%
TACTCGTCCTGTACCGAAAT+GGG - chr1.2:71345283-71345302 MS.gene60735:intron 45.0%
TAGGCTAAATCATTTCCGCG+AGG - chr1.2:71345619-71345638 MS.gene60735:CDS 45.0%
TAGTCACCATCTGATTCAGC+CGG + chr1.2:71344290-71344309 None:intergenic 45.0%
TCACATGAATTGTGCTCCAG+AGG + chr1.2:71346948-71346967 None:intergenic 45.0%
TGAACTGCAGAATTTCTCCC+AGG - chr1.2:71344592-71344611 MS.gene60735:CDS 45.0%
TGCCATATATGTGGCAGTCA+CGG + chr1.2:71347755-71347774 None:intergenic 45.0%
TGCGACCGTATTCTATCACA+TGG - chr1.2:71347670-71347689 MS.gene60735:intron 45.0%
TGGAGATGGAGGATCACTAA+AGG + chr1.2:71345366-71345385 None:intergenic 45.0%
TGTGTTGGGACATGGAATGT+TGG - chr1.2:71344774-71344793 MS.gene60735:intron 45.0%
TTGGGTGTATTGTGCAGAGT+GGG - chr1.2:71344223-71344242 MS.gene60735:intron 45.0%
TTTCCGATGAATCTTCCTCG+CGG + chr1.2:71345637-71345656 None:intergenic 45.0%
TTTCCGCGAGGAAGATTCAT+CGG - chr1.2:71345631-71345650 MS.gene60735:CDS 45.0%
TTTGGGTGTATTGTGCAGAG+TGG - chr1.2:71344222-71344241 MS.gene60735:intron 45.0%
! ACTTTTGCCTCCTTAAGCCT+GGG + chr1.2:71344612-71344631 None:intergenic 45.0%
! GAGTACCTGTTGTTGCTTGT+TGG + chr1.2:71343579-71343598 None:intergenic 45.0%
!! GGGACTTGATTTGTGCAAGT+TGG - chr1.2:71343737-71343756 MS.gene60735:CDS 45.0%
!! TCCAGAGGAAGGTGTTAATG+CGG + chr1.2:71346933-71346952 None:intergenic 45.0%
!!! AATCATTTTTTTTTTTTTTA+TGG - chr1.2:71344963-71344982 MS.gene60735:CDS 5.0%
!!! TATTTTATTTATATTTTTCA+GGG - chr1.2:71346269-71346288 MS.gene60735:intron 5.0%
!!! TTATTTTATTTATATTTTTC+AGG - chr1.2:71346268-71346287 MS.gene60735:intron 5.0%
ACCCGTGACTGCCACATATA+TGG - chr1.2:71347750-71347769 MS.gene60735:intron 50.0%
ACCGCATTAACACCTTCCTC+TGG - chr1.2:71346929-71346948 MS.gene60735:intron 50.0%
AGAATACGGTCGCACCTGTA+AGG + chr1.2:71347664-71347683 None:intergenic 50.0%
AGTGCTCGCTGTAGCTTCTT+TGG + chr1.2:71347796-71347815 None:intergenic 50.0%
CACAGCCAACAAGCAACAAC+AGG - chr1.2:71343571-71343590 MS.gene60735:CDS 50.0%
GACTGCCACATATATGGCAG+AGG - chr1.2:71347756-71347775 MS.gene60735:intron 50.0%
GCCATATATGTGGCAGTCAC+GGG + chr1.2:71347754-71347773 None:intergenic 50.0%
GTACATTGGAGAGGACCCAA+AGG - chr1.2:71347393-71347412 MS.gene60735:intron 50.0%
GTTGGGACATGGAATGTTGG+AGG - chr1.2:71344777-71344796 MS.gene60735:intron 50.0%
TCACGACAAGTTTCGGCACT+TGG - chr1.2:71347851-71347870 MS.gene60735:CDS 50.0%
TCATCACGGTATGGGTTCGA+AGG - chr1.2:71346041-71346060 MS.gene60735:CDS 50.0%
TGGGTGTATTGTGCAGAGTG+GGG - chr1.2:71344224-71344243 MS.gene60735:intron 50.0%
! CTTCCTTTTCACCTGCCGTA+AGG + chr1.2:71346352-71346371 None:intergenic 50.0%
! CTTTTCACCTGCCGTAAGGT+GGG + chr1.2:71346348-71346367 None:intergenic 50.0%
! GACTTTTGCCTCCTTAAGCC+TGG + chr1.2:71344613-71344632 None:intergenic 50.0%
ATATGCACCCACCTTACGGC+AGG - chr1.2:71346338-71346357 MS.gene60735:intron 55.0%
ATGTGGCAGTCACGGGTCTA+TGG + chr1.2:71347747-71347766 None:intergenic 55.0%
ATTGTGCAGAGTGGGGAAGC+CGG - chr1.2:71344231-71344250 MS.gene60735:intron 55.0%
CAGGAGAGAGGATGAAGCTC+CGG - chr1.2:71344268-71344287 MS.gene60735:intron 55.0%
CCACCTTACGGCAGGTGAAA+AGG - chr1.2:71346346-71346365 MS.gene60735:intron 55.0%
GAAGCTCCGGCTGAATCAGA+TGG - chr1.2:71344281-71344300 MS.gene60735:intron 55.0%
GTGTACGCCTCGCAACCTTT+GGG + chr1.2:71347411-71347430 None:intergenic 55.0%
TATGGGTTCGAAGGAGCCTG+CGG - chr1.2:71346050-71346069 MS.gene60735:CDS 55.0%
TCCTGTCCCTAATCCGTGAC+CGG + chr1.2:71344253-71344272 None:intergenic 55.0%
! CCTTTTCACCTGCCGTAAGG+TGG + chr1.2:71346349-71346368 None:intergenic 55.0%
!! ACGGATTAGGGACAGGAGAG+AGG - chr1.2:71344256-71344275 MS.gene60735:intron 55.0%
GAGAGGACCCAAAGGTTGCG+AGG - chr1.2:71347401-71347420 MS.gene60735:intron 60.0%
GGTGTACGCCTCGCAACCTT+TGG + chr1.2:71347412-71347431 None:intergenic 60.0%
TTGCGAGGCGTACACCAGCA+TGG - chr1.2:71347416-71347435 MS.gene60735:intron 60.0%
! GGGAAGCCGGTCACGGATTA+GGG - chr1.2:71344244-71344263 MS.gene60735:intron 60.0%
CAGAGTGGGGAAGCCGGTCA+CGG - chr1.2:71344237-71344256 MS.gene60735:intron 65.0%
GGGGAAGCCGGTCACGGATT+AGG - chr1.2:71344243-71344262 MS.gene60735:intron 65.0%
!! GCCGGTCACGGATTAGGGAC+AGG - chr1.2:71344249-71344268 MS.gene60735:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 71343548 71347888 71343548 ID=MS.gene60735
chr1.2 mRNA 71343548 71347888 71343548 ID=MS.gene60735.t1;Parent=MS.gene60735
chr1.2 exon 71347844 71347888 71347844 ID=MS.gene60735.t1.exon1;Parent=MS.gene60735.t1
chr1.2 CDS 71347844 71347888 71347844 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71347460 71347592 71347460 ID=MS.gene60735.t1.exon2;Parent=MS.gene60735.t1
chr1.2 CDS 71347460 71347592 71347460 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71347134 71347196 71347134 ID=MS.gene60735.t1.exon3;Parent=MS.gene60735.t1
chr1.2 CDS 71347134 71347196 71347134 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71346762 71346834 71346762 ID=MS.gene60735.t1.exon4;Parent=MS.gene60735.t1
chr1.2 CDS 71346762 71346834 71346762 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71346562 71346666 71346562 ID=MS.gene60735.t1.exon5;Parent=MS.gene60735.t1
chr1.2 CDS 71346562 71346666 71346562 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71345298 71346204 71345298 ID=MS.gene60735.t1.exon6;Parent=MS.gene60735.t1
chr1.2 CDS 71345298 71346204 71345298 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71344962 71345146 71344962 ID=MS.gene60735.t1.exon7;Parent=MS.gene60735.t1
chr1.2 CDS 71344962 71345146 71344962 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71344555 71344663 71344555 ID=MS.gene60735.t1.exon8;Parent=MS.gene60735.t1
chr1.2 CDS 71344555 71344663 71344555 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71343999 71344138 71343999 ID=MS.gene60735.t1.exon9;Parent=MS.gene60735.t1
chr1.2 CDS 71343999 71344138 71343999 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
chr1.2 exon 71343548 71343767 71343548 ID=MS.gene60735.t1.exon10;Parent=MS.gene60735.t1
chr1.2 CDS 71343548 71343767 71343548 ID=cds.MS.gene60735.t1;Parent=MS.gene60735.t1
Gene Sequence

>MS.gene60735

ATGAAGCAGGAACAGCAGAAGCACACAGCCAACAAGCAACAACAGGTACTCTTTCTTTGTTCAGCCTTGCATGGCTTATTTTTATACTGGATACCGGCCTAACTCATACTCATCCTTACAAAACCGGCTTGTAAGAGGATGAGTGTATTTCTTTATAAATTCTAGTCGGACCTTATCTATAATCTATGTGGGACTTGATTTGTGCAAGTTGGTGTTTGTGAGTATTTGGAAACTGAGGCAAATTGCAGTGAAATTGAGCTGAAGTGTATATTTTCTGGCTTCGTCTTTGTTTATAGAATTTATGGGCAACAATGGTCATGCGCAAATGGCTTAACATTAAAAGGAAGGAGTCTGATGATTACAGCACCGACCCTGATGATGATGATGATGATGATGAGGATGATCCTGAAACTGATTCAGACAATGAAGGTTAGTCTATTTAGTGATGTTTCTATTGTAATTAATAAAACATTGGTGTGAAATTTTGTCACCGTGTCATTAGCAGAAGACTATTCATTTGAAGATTTGGAAATTGAGGATTATGGTTTGTTTCTAACTAATGATCTGAAAATTCAGATTTGTGATTGATTTGAGATCTTAGAAACCATGTCTTGTGTGTGATGAATGATGATTCTGGTTCAGTTTGGTTTTTTCTGTGATCTGTCTCAATTTTATTTGGGTGTATTGTGCAGAGTGGGGAAGCCGGTCACGGATTAGGGACAGGAGAGAGGATGAAGCTCCGGCTGAATCAGATGGTGACTATTTTCTTTCATATATGAAATGATAACAGACAAGTTTTACTGATTTTGTCTTAATTTCAAGACTAGTATAATGCCGTCGGATGGTGATACATCATTATAGTTTTGAAACTAGCACTCGTTAGTTTTGACAATTCTTCCTGTATTAGGCCATTCGAAATTTGTGGATTCCCTAAATTTAAATCGAAAAATGGAACCTCATATTCGACTTTTGAGAGCTAAAATACATATCAAAATCAAATAAGTATTTTCAGCAATAAAAGCATGAACTCTGAATAATTTCAAATGAACTGCAGAATTTCTCCCAGGCTTAAGGAGGCAAAAGTCATTGACTTTAAGGTCTCAATATATAAACAAGAAGGAACTGAGGTTAGGAACTGCTCTTGTCTTAGAAAGCATAGTTTGTTTTTGTTATTTCTCACAAGCCTCTCATATCTGAATTCTGTTGTATACTTTTCTATTAGAGTATGTGTTGGGACATGGAATGTTGGAGGAAAACTTCCACCTAATGACTTTGATATTGATGATTGGCTAGACATTAACCATCCTGCTGATATCTATGTCCTTGGGTAAGTTTCACACTAAATTTTCTCACTATTGCATATACACTCAAATATAACGCCATTTTGAAATCACTTTTAATACATTTTCTTGCATAATCATTTTTTTTTTTTTTATGGATTTGATTCACATGCACGTTCAGTGTAAAGATTCTTTACACTGTCGATCAATTGTCATTGTCTGAACATGAAAAATGTTTGACTTTAATCATAATTTGCTTCTAAAGTCACGCGTATGATTGTTTCTGATTTATTGATAGTATAAAATTTTCTGCACTGACTGTGTATCAAAAGTGTTTTTATATACACTTGGTGGTTTGTCTGATTTGTTTGACTAAAAAGTTTCTTTGCACTTTGCAATTGCAGTCTTCAAGAGATTGTACCTTTAAACACGAGTAACATTTTTGTTGCTGTAGATACTCGTCCTGTACCGAAATGGGAAAACTTAATTCGAGAAGCTCTGAATAGAGTTCGATCAAAACCTTCAAAGATAAAATCCTTTAGTGATCCTCCATCTCCATCAAAGTTTAAACCATCAGATGATGCACCAGATATAGAAGAAGAAATTCTATTAGAAAGCGATAGTGATATTGGTGAGGAGATTCATCCTTTAGGTGAAGAACATAATTTATTTGGAGTCACAGATAAACCAATCATAGATGAAGCACTGAATATTAGTTTGAAGGATTCAAATGCTTCTGATATAGCTGAGAACGATCTACAGAATCAGTTATCTTATCAAAGAAGGTTGAATAGGCTAAATCATTTCCGCGAGGAAGATTCATCGGAAAACAATGAAACAACTTCTTCTCAACAGATTAGTAAGCTATCTAGAATGATTAATGGGACTGAAAAAGATTCATCGGAAAACAATGAAACGACTTCTTCTCAACAGATTAGTAAGCTATCTAGAATGGTTAGTGGGACTGAAAGAATTGGTTTGAGCTGGCCAGAGCCACCATTACATTTGTTGCCTCAGAAAGTATTGGAGAGACCAACTTCTTTTAAACCGGTTAAATCGTTTAATAAGACAAAGTCATTTAGAGCCTGTGATTCTTTCAAATCAAAAACAGATGCTATAGATTTACTGGCTGATATTGATCTTGAAGCTTTGATGAAGAGGAAAACAAGATCGTCGTATGTGAAGATAGTGAGCAAGCAGATGGTTGGTATTTTCATCACGGTATGGGTTCGAAGGAGCCTGCGGAAACATATTCAGAACATGAAGGTTTCAACTGTTGGTGTTGGTGTTATGGGCTATATTGGTAACAAGGTCTCTTTCACTTCCTTTGAAATTTTCCCTAGAGTTTGTAGTTTTTGGTCATTACATTGTCATTAACATATTCATATGAGAAATTGAGAATGATAACATATATGAGAAATGTTATCAATGATGAGAAACTTATTTTATTTATATTTTTCAGGGATCAATATCTGTCAGCATGTCTATATATCAGACGCTTTTTTGCTTTATATGCACCCACCTTACGGCAGGTGAAAAGGAAGCCGATGAAATCAAACGAAATGCTGATGTTCGTGAAATACATCAGAGGACCCATTTTTATTCACTTTCTGATTTTGGACTTCCCAAAAGTATCTTGGATCATGAGTGAGTTCATGTTTTACATACCACATTCAAATATGTTAATTTAATTTTACTCCTTGAATTATATGCTAATGCCTTTTGATTAAAATTAAGGTAATATATAAAACCAATGTCTTGAATTTACATGGCTAGAATTTAACTTGAACATTTTTTTTTATCCTTCTATATGATTAAATTAGAACCTTCTATTGAAGTAAATTAATCTGCAAATTCTGTTTCTGCTTGGCTGCTGAAATTCTGTATTCACTGGTTCTGTTTGAAAAAATTATAATCATTTAATCAAATTCTAATTTATTTGCAGAAGAATAATTTGGTTCGGTGATCTGAATTACCGTATCAGCTTGCCATATGATAAAACAAGAGATCTTATCTCAAAAAAGCATTGGTCAAAATTGGTTGAGAGAGACCAGGTAATATACCCTTCCATTAGATGTATCAACTTGATACTATCAAGTGTACCGCATTAACACCTTCCTCTGGAGCACAATTCATGTGATTATTGATGTCTTATTTCTGAGGAGTAATAAGCAAAATCCATTAAGAAATATATGTTCCGTCACTCAGTAAATTCATCGACATCATTTCATCGTAAACTTGCAATTCATTAATTGAAATACTACAAGTTAAGAGCATCACTTGAATAACAACTTATGTAAAAACTACTCGAGTAAATAAAACTTTATGTTGATATTTATATCCATTTTGTAACATAGATAATCGTATTTAAGGGTTTAGGGTTTCATGATATTTATATCCTTATTCATTTTGATTTCATGATATAGATTTTGAACTCAAGTTAAAAGGTATTGACATCTTACTATGACAGCTTGTGAAAGAATTAGAGAAAGGTGTTTTTGATGGATGGTCAGAAGGTACGTTAAACTTCCCACCAACCTATAAGTACGAGATTAATTCAGACAAGTACATTGGAGAGGACCCAAAGGTTGCGAGGCGTACACCAGCATGGTATGCTTTAGTTTGCTTCAACAGAGATTCTGCTTCAATTTGTGTTTTATATGCATTTATTCATGTATTAATTTACCATTTTTTATCATACTCATAAACCAAATTGTTCTTAGTAAAATGAACTACTAATAATATTAATACTGCTGTGTGTGTTCGTGTATATGCATGACTATACTATACATGATTATTTATAAATGAATTTTCTTTTTAATGTTTGCAATTACCTTACAGGTGCGACCGTATTCTATCACATGGAAATGGAATGAGATTACTGAGTTACAAAAGGAGTGAACTTAAATTTTCAGACCATAGACCCGTGACTGCCACATATATGGCAGAGGTTGAGGTGTTCAATCCAAAGAAGCTACAGCGAGCACTTAATTACACCGATGCAGAGATCGAAAATGAAGAAGTCACGACAAGTTTCGGCACTTGGAATTTAGCAACATAA
Protein sequence

>MS.gene60735.t1

MKQEQQKHTANKQQQNLWATMVMRKWLNIKRKESDDYSTDPDDDDDDDEDDPETDSDNEEWGSRSRIRDRREDEAPAESDEFLPGLRRQKSLTLRSQYINKKELRVCVGTWNVGGKLPPNDFDIDDWLDINHPADIYVLGLQEIVPLNTSNIFVAVDTRPVPKWENLIREALNRVRSKPSKIKSFSDPPSPSKFKPSDDAPDIEEEILLESDSDIGEEIHPLGEEHNLFGVTDKPIIDEALNISLKDSNASDIAENDLQNQLSYQRRLNRLNHFREEDSSENNETTSSQQISKLSRMINGTEKDSSENNETTSSQQISKLSRMVSGTERIGLSWPEPPLHLLPQKVLERPTSFKPVKSFNKTKSFRACDSFKSKTDAIDLLADIDLEALMKRKTRSSYVKIVSKQMVGIFITVWVRRSLRKHIQNMKVSTVGVGVMGYIGNKGSISVSMSIYQTLFCFICTHLTAGEKEADEIKRNADVREIHQRTHFYSLSDFGLPKSILDHERIIWFGDLNYRISLPYDKTRDLISKKHWSKLVERDQLVKELEKGVFDGWSEGTLNFPPTYKYEINSDKYIGEDPKVARRTPAWCDRILSHGNGMRLLSYKRSELKFSDHRPVTATYMAEVEVFNPKKLQRALNYTDAEIENEEVTTSFGTWNLAT