Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60879.t1 | XP_013454678.1 | 98.4 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 5.90E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60879.t1 | O13801 | 32.0 | 150 | 47 | 5 | 3 | 98 | 343 | 491 | 6.9e-09 | 61.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60879.t1 | A0A072UG31 | 98.4 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 4.3e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene60879 | 0.848326 | 6.58E-60 | -1.69E-46 |
| MS.gene051478 | MS.gene60879 | 0.802356 | 5.83E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene60879 | 0.807099 | 5.96E-50 | -1.69E-46 |
| MS.gene056228 | MS.gene60879 | 0.803243 | 3.83E-49 | -1.69E-46 |
| MS.gene059300 | MS.gene60879 | 0.80028 | 1.55E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60879.t1 | MTR_4g011190 | 98.361 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 6.19e-84 | 241 |
| MS.gene60879.t1 | MTR_3g107610 | 59.124 | 137 | 41 | 5 | 1 | 122 | 1 | 137 | 4.05e-42 | 137 |
| MS.gene60879.t1 | MTR_3g107610 | 59.124 | 137 | 41 | 5 | 1 | 122 | 1 | 137 | 1.10e-41 | 135 |
| MS.gene60879.t1 | MTR_4g113840 | 44.538 | 119 | 51 | 3 | 5 | 121 | 6 | 111 | 3.82e-28 | 100 |
| MS.gene60879.t1 | MTR_2g054750 | 40.678 | 118 | 47 | 2 | 5 | 122 | 6 | 100 | 3.03e-24 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60879.t1 | AT3G15680 | 58.824 | 136 | 40 | 5 | 1 | 122 | 1 | 134 | 5.41e-42 | 136 |
| MS.gene60879.t1 | AT3G15680 | 58.824 | 136 | 40 | 5 | 1 | 122 | 1 | 134 | 5.41e-42 | 136 |
| MS.gene60879.t1 | AT5G25490 | 51.799 | 139 | 49 | 4 | 1 | 121 | 1 | 139 | 6.12e-34 | 116 |
| MS.gene60879.t1 | AT5G25490 | 51.799 | 139 | 49 | 4 | 1 | 121 | 1 | 139 | 6.12e-34 | 116 |
| MS.gene60879.t1 | AT2G26695 | 44.444 | 117 | 53 | 3 | 5 | 121 | 6 | 110 | 1.09e-28 | 102 |
| MS.gene60879.t1 | AT2G26695 | 44.444 | 117 | 53 | 3 | 5 | 121 | 6 | 110 | 1.09e-28 | 102 |
Find 0 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTGATTATGGATCAATGTT+TGG | + | 29354:9022-9041 | MS.gene60879:CDS | 30.0% | |
| ! | ATTTTGCTTTCAAGAGGTTT+TGG | + | 29354:9217-9236 | MS.gene60879:CDS | 30.0% |
| ATTAGAGGAGGATCTTCATT+TGG | + | 29354:9049-9068 | MS.gene60879:CDS | 35.0% | |
| GATTATGGATCAATGTTTGG+AGG | + | 29354:9025-9044 | MS.gene60879:CDS | 35.0% | |
| TCAATGTTTGGAGGAATTAG+AGG | + | 29354:9034-9053 | MS.gene60879:CDS | 35.0% | |
| ! | AGTGACATTTTGCTTTCAAG+AGG | + | 29354:9211-9230 | MS.gene60879:CDS | 35.0% |
| ! | GGATCTTCATTTGGTTTAAG+TGG | + | 29354:9058-9077 | MS.gene60879:CDS | 35.0% |
| ! | TATGGAGACAGAGTTGATTA+TGG | + | 29354:9010-9029 | MS.gene60879:CDS | 35.0% |
| !! | CGTTTAAGTTGCTTCAAATG+TGG | + | 29354:9148-9167 | MS.gene60879:CDS | 35.0% |
| AGTGGTTCAGATGTTCGTTT+AGG | + | 29354:9076-9095 | MS.gene60879:CDS | 40.0% | |
| ATGTTTGGAGGAATTAGAGG+AGG | + | 29354:9037-9056 | MS.gene60879:CDS | 40.0% | |
| CAGATGTTCGTTTAGGTGAT+TGG | + | 29354:9083-9102 | MS.gene60879:CDS | 40.0% | |
| CGTTTAGGTGATTGGTATTG+TGG | + | 29354:9091-9110 | MS.gene60879:CDS | 40.0% | |
| GCCAACACATGAACTTTCAA+AGG | + | 29354:8954-8973 | MS.gene60879:CDS | 40.0% | |
| TATTATATCCTCCACCAGCT+AGG | - | 29354:9189-9208 | MS.gene60879:intergenic | 40.0% | |
| ! | GCCTTTGAAAGTTCATGTGT+TGG | - | 29354:8958-8977 | MS.gene60879:intergenic | 40.0% |
| !! | TATTGTGGTGCTGGTAATTG+TGG | + | 29354:9106-9125 | MS.gene60879:CDS | 40.0% |
| !! | TTGCTTTCAAGAGGTTTTGG+TGG | + | 29354:9220-9239 | MS.gene60879:CDS | 40.0% |
| ACTAGACCTGGATGGAAATC+TGG | + | 29354:9247-9266 | MS.gene60879:CDS | 45.0% | |
| AGATGCGGCGATTCAAAGTA+TGG | + | 29354:8992-9011 | MS.gene60879:CDS | 45.0% | |
| CAATCACCAGATTTCCATCC+AGG | - | 29354:9256-9275 | MS.gene60879:intergenic | 45.0% | |
| CTGGATGGAAATCTGGTGAT+TGG | + | 29354:9254-9273 | MS.gene60879:CDS | 45.0% | |
| GCTTTCAAAGATGACCTAGC+TGG | + | 29354:9172-9191 | MS.gene60879:CDS | 45.0% | |
| TTCAAAGATGACCTAGCTGG+TGG | + | 29354:9175-9194 | MS.gene60879:CDS | 45.0% | |
| !! | TTTGGTGGAAGTACTAGACC+TGG | + | 29354:9235-9254 | MS.gene60879:CDS | 45.0% |
| AAAGATGACCTAGCTGGTGG+AGG | + | 29354:9178-9197 | MS.gene60879:CDS | 50.0% | |
| CGCGAATCGTGTCAAAGATG+CGG | + | 29354:8977-8996 | MS.gene60879:CDS | 50.0% | |
| GGTGATTGGTATTGTGGTGC+TGG | + | 29354:9097-9116 | MS.gene60879:CDS | 50.0% | |
| !! | GTGGAAGTACTAGACCTGGA+TGG | + | 29354:9239-9258 | MS.gene60879:CDS | 50.0% |
| GACCTGCAGTTCCAATCTCC+TGG | - | 29354:8932-8951 | MS.gene60879:intergenic | 55.0% | |
| GGCCAGGAGATTGGAACTGC+AGG | + | 29354:8927-8946 | MS.gene60879:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 29354 | gene | 8920 | 9287 | 8920 | ID=MS.gene60879 |
| 29354 | mRNA | 8920 | 9287 | 8920 | ID=MS.gene60879.t1;Parent=MS.gene60879 |
| 29354 | exon | 8920 | 9287 | 8920 | ID=MS.gene60879.t1.exon1;Parent=MS.gene60879.t1 |
| 29354 | CDS | 8920 | 9287 | 8920 | ID=cds.MS.gene60879.t1;Parent=MS.gene60879.t1 |
| Gene Sequence |
| Protein sequence |
