AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60894

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60894.t1 MTR_2g062560 97.032 438 13 0 1 438 131 568 0.0 893
MS.gene60894.t1 MTR_7g023060 31.982 444 234 12 1 432 168 555 1.52e-62 212
MS.gene60894.t1 MTR_1g115475 31.757 444 235 12 1 432 146 533 2.12e-62 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60894.t1 AT1G05590 69.773 440 126 3 1 437 140 575 0.0 662
MS.gene60894.t1 AT1G05590 69.773 440 126 3 1 437 140 575 0.0 662
MS.gene60894.t1 AT1G05590 69.773 440 126 3 1 437 140 575 0.0 662
MS.gene60894.t1 AT3G55260 31.222 442 240 11 1 432 151 538 3.07e-62 211
MS.gene60894.t1 AT1G65590 30.445 427 234 11 1 420 148 518 2.54e-59 202

Find 112 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAAAAGGGTTGTTGAATT+TGG 0.169426 2.3:-31921225 MS.gene60894:CDS
GTTCTTAACAAATCCTTTAC+TGG 0.243313 2.3:+31921393 None:intergenic
ATACGGTTGATGATGTTAAA+AGG 0.243332 2.3:-31921239 MS.gene60894:CDS
TTGGTAGGTCTTGGGGTTTA+AGG 0.248007 2.3:+31919689 None:intergenic
TTAAAAGGGTTGTTGAATTT+GGG 0.272932 2.3:-31921224 MS.gene60894:CDS
TACAATTATGATATAACTTA+TGG 0.278390 2.3:-31919180 MS.gene60894:CDS
CTGCAGGACTTGGTAGGTCT+TGG 0.282162 2.3:+31919680 None:intergenic
TGATGTTAATATGGTTTATA+CGG 0.282266 2.3:-31921256 MS.gene60894:CDS
TGCAGGACTTGGTAGGTCTT+GGG 0.284268 2.3:+31919681 None:intergenic
TGTGGACACGGCGACTTTAC+TGG 0.284986 2.3:-31919291 MS.gene60894:CDS
ACTAGCCGATGAAACTGTTT+TGG 0.294927 2.3:-31919100 MS.gene60894:CDS
GACCGAAGAAGAAGCGAAAT+TGG 0.298472 2.3:-31919154 MS.gene60894:CDS
CAGATATTTCTATCAAATAA+TGG 0.299029 2.3:-31919558 MS.gene60894:CDS
CTCTCCAAACACAAACTAAA+TGG 0.300512 2.3:+31918873 None:intergenic
ATGGACATGACAACACTAAA+AGG 0.308012 2.3:-31919371 MS.gene60894:CDS
AGGTGCTTATGATGTTAATA+TGG 0.311318 2.3:-31921265 MS.gene60894:CDS
CAACCGTACTGTTGTCTATT+GGG 0.333502 2.3:-31919475 MS.gene60894:CDS
TGGTAGGTCTTGGGGTTTAA+GGG 0.334909 2.3:+31919690 None:intergenic
GATAGAAATATCTGAATTGT+TGG 0.336236 2.3:+31919567 None:intergenic
TTCAACCACTTTGGTGTGTT+AGG 0.344290 2.3:-31918924 MS.gene60894:CDS
TCAACCGTACTGTTGTCTAT+TGG 0.350488 2.3:-31919476 MS.gene60894:CDS
GTCAATGACGCGCCGTTGTT+TGG 0.358926 2.3:-31921456 MS.gene60894:CDS
AGTCCTTGTGGTCAGGCAAT+AGG 0.362517 2.3:-31919041 MS.gene60894:CDS
CCAGAATGGTAAAATTGTTC+CGG 0.373560 2.3:+31919627 None:intergenic
GGCTCTGTTGCAAGACGATC+TGG 0.380818 2.3:+31919732 None:intergenic
AACCAATTTCGCTTCTTCTT+CGG 0.390626 2.3:+31919152 None:intergenic
CGTTCACAGCCACGCGCGTT+GGG 0.392840 2.3:+31921484 None:intergenic
TCAGCCATTGCAGAAGTTCT+AGG 0.394283 2.3:+31919063 None:intergenic
TTAGCACATGTTACAATGTC+AGG 0.402865 2.3:+31919786 None:intergenic
ATGACGTCGTACCAGGTTGC+TGG 0.406537 2.3:-31919599 MS.gene60894:CDS
GTCCTTGTGGTCAGGCAATA+GGG 0.416098 2.3:-31919040 MS.gene60894:CDS
CGATGTAACCACATTGTTCC+CGG 0.418185 2.3:-31919646 MS.gene60894:CDS
TAACATGTGCTAACATGTTC+TGG 0.425511 2.3:-31919776 MS.gene60894:CDS
GTCAGGCAATAGGGATGAAA+AGG 0.425841 2.3:-31919031 MS.gene60894:CDS
GGATTTAAATGACCTGTTCC+CGG 0.426058 2.3:+31919711 None:intergenic
ACACACCAAAGTGGTTGAAT+AGG 0.427892 2.3:+31918928 None:intergenic
ACGATCTGGCCAATCGCTTC+CGG 0.428599 2.3:+31919746 None:intergenic
TCTTTAGTGAGAATCGTTGA+TGG 0.430361 2.3:+31919420 None:intergenic
AGAATGGTGAGTAGAGGTAT+AGG 0.430719 2.3:-31918958 MS.gene60894:CDS
TACGGTTGATGATGTTAAAA+GGG 0.430970 2.3:-31921238 MS.gene60894:CDS
TTCATATATGCAGGGCATAC+AGG 0.441868 2.3:-31919828 MS.gene60894:intron
CTTGCAACAGAGCCGGGAAC+AGG 0.445919 2.3:-31919723 MS.gene60894:CDS
CAGAATGGTAAAATTGTTCC+GGG 0.446032 2.3:+31919628 None:intergenic
ATAAGTTGAATGTGTTTCAT+TGG 0.450469 2.3:-31921350 MS.gene60894:CDS
CAACAGATAGATTGAATGAA+TGG 0.452558 2.3:-31918987 MS.gene60894:CDS
AGATCGTCTTGCAACAGAGC+CGG 0.466886 2.3:-31919730 MS.gene60894:CDS
TCTTCCTTCTGAACCAATGT+TGG 0.467174 2.3:-31921295 MS.gene60894:CDS
TTGCCTGAGATTGATGCTCC+TGG 0.467453 2.3:-31921180 MS.gene60894:intron
AGTATTTACGACAACCAAAC+AGG 0.473889 2.3:-31919261 MS.gene60894:CDS
TGTACTGTGTTGCACATACC+AGG 0.474032 2.3:+31918898 None:intergenic
ACTAAAAGGATAGTATCTTC+TGG 0.481580 2.3:-31919357 MS.gene60894:CDS
GTATGACATTCTGCAGGACT+TGG 0.482257 2.3:+31919670 None:intergenic
GATCGTCTTGCAACAGAGCC+GGG 0.487708 2.3:-31919729 MS.gene60894:CDS
GCAGGACTTGGTAGGTCTTG+GGG 0.489357 2.3:+31919682 None:intergenic
TGATAAATGTCTCAAGGACT+TGG 0.493167 2.3:+31919526 None:intergenic
GGTGCTGATGACGTCGTACC+AGG 0.495012 2.3:-31919606 MS.gene60894:CDS
ATGCAGGGCATACAGGATCA+TGG 0.495303 2.3:-31919821 MS.gene60894:intron
GGTGGCCAGCCGGAAGCGAT+TGG 0.500162 2.3:-31919755 MS.gene60894:CDS
ATGGCTGAGTCCTTGTGGTC+AGG 0.502527 2.3:-31919048 MS.gene60894:CDS
AAACGTTCTCACAGCTTGCA+TGG 0.506858 2.3:-31921512 MS.gene60894:CDS
AGAAGCAGAATGGTGAGTAG+AGG 0.507345 2.3:-31918964 MS.gene60894:CDS
ACGTTCACAGCCACGCGCGT+TGG 0.511117 2.3:+31921483 None:intergenic
TCTGGCCAATCGCTTCCGGC+TGG 0.511465 2.3:+31919750 None:intergenic
AATAGACAACAGTACGGTTG+AGG 0.513519 2.3:+31919478 None:intergenic
AACGTTCTCACAGCTTGCAT+GGG 0.515119 2.3:-31921511 MS.gene60894:CDS
AACATGTTCTGGTGGCCAGC+CGG 0.515403 2.3:-31919765 MS.gene60894:CDS
AAGAAACTACTATCCAGTAA+AGG 0.519716 2.3:-31921406 MS.gene60894:CDS
CTGAGCCTATTCAACCACTT+TGG 0.522499 2.3:-31918933 MS.gene60894:CDS
TATTATCTTGACTGTGGACA+CGG 0.524989 2.3:-31919303 MS.gene60894:CDS
ATAGACAACAGTACGGTTGA+GGG 0.530612 2.3:+31919479 None:intergenic
TAACCAGGAGCATCAATCTC+AGG 0.535232 2.3:+31921177 None:intergenic
AACGATGGCGGCTCTTGGTG+TGG 0.535553 2.3:-31919228 MS.gene60894:CDS
ATCCCTATTGCCTGACCACA+AGG 0.540723 2.3:+31919038 None:intergenic
TCAGCCAACATTGGTTCAGA+AGG 0.540910 2.3:+31921291 None:intergenic
AAATATCTGAATTGTTGGAT+CGG 0.543106 2.3:+31919572 None:intergenic
ACCTCATGGGGAGCCATGCA+TGG 0.551149 2.3:-31921540 None:intergenic
CTTTGGTGTGTTAGGAACCC+TGG 0.555049 2.3:-31918916 MS.gene60894:CDS
TGCAGGGCATACAGGATCAT+GGG 0.555993 2.3:-31919820 MS.gene60894:intron
GGTTCAGAAGGAAGAATCAA+AGG 0.559179 2.3:+31921303 None:intergenic
CGGTTGAGGGATAAAATGAA+AGG 0.561852 2.3:+31919492 None:intergenic
TACAATGTCAGGGTAGGCTA+AGG 0.562452 2.3:+31919797 None:intergenic
GCACCTTTCTCAGCCAACAT+TGG 0.566807 2.3:+31921282 None:intergenic
CTGCAATGGCTGAGTCCTTG+TGG 0.573090 2.3:-31919053 MS.gene60894:CDS
CGAATCCAAAACAGTTTCAT+CGG 0.574123 2.3:+31919095 None:intergenic
GAACCAATGTTGGCTGAGAA+AGG 0.574287 2.3:-31921285 MS.gene60894:CDS
TGAGAATCGTTGATGGAACA+TGG 0.576260 2.3:+31919427 None:intergenic
CGACGTCATCAGCACCAGAA+TGG 0.582990 2.3:+31919613 None:intergenic
GGTTGAGGGATAAAATGAAA+GGG 0.590587 2.3:+31919493 None:intergenic
ATGGGGAAACCCAACGCGCG+TGG 0.606424 2.3:-31921493 MS.gene60894:CDS
TTGGCCTAGAACTTCTGCAA+TGG 0.607551 2.3:-31919067 MS.gene60894:CDS
GACTTCTATTATCTTGACTG+TGG 0.608516 2.3:-31919309 MS.gene60894:CDS
GTTGAATTTGGGCTTGATCG+TGG 0.612314 2.3:-31921213 MS.gene60894:CDS
GAACGTTTCTAAACCATGCA+TGG 0.616273 2.3:+31921527 None:intergenic
TGTTGTTGATAAATGTCTCA+AGG 0.621150 2.3:+31919520 None:intergenic
ACATGTTACAATGTCAGGGT+AGG 0.627325 2.3:+31919791 None:intergenic
TCAGGCAATAGGGATGAAAA+GGG 0.628998 2.3:-31919030 MS.gene60894:CDS
GAATGAATGGAGAAGCAGAA+TGG 0.631006 2.3:-31918974 MS.gene60894:CDS
TAGCACATGTTACAATGTCA+GGG 0.638552 2.3:+31919787 None:intergenic
GGGTCTGAAGCGATACGCAG+AGG 0.646523 2.3:-31919010 MS.gene60894:CDS
CTGATGATGACACGATGGCG+CGG 0.650072 2.3:+31919331 None:intergenic
CATCGCGTATGACATTCTGC+AGG 0.653326 2.3:+31919664 None:intergenic
GACATTCTGCAGGACTTGGT+AGG 0.653628 2.3:+31919674 None:intergenic
ACGTTCTCACAGCTTGCATG+GGG 0.658259 2.3:-31921510 MS.gene60894:CDS
CTTCCCAATAGACAACAGTA+CGG 0.678240 2.3:+31919472 None:intergenic
GGGTTCCTAACACACCAAAG+TGG 0.688027 2.3:+31918919 None:intergenic
ACACCGCGATGACCAAACAA+CGG 0.696045 2.3:+31921444 None:intergenic
GCGCCGTTGTTTGGTCATCG+CGG 0.698629 2.3:-31921447 MS.gene60894:CDS
GAAGTCTGATGATGACACGA+TGG 0.708731 2.3:+31919326 None:intergenic
CATGTGCTAACATGTTCTGG+TGG 0.723594 2.3:-31919773 MS.gene60894:CDS
TAGGTGGGGAGGTAGCGTTG+TGG 0.723910 2.3:-31919128 MS.gene60894:CDS
GTACTGTGTTGCACATACCA+GGG 0.730881 2.3:+31918899 None:intergenic
AAATTGTTCCGGGAACAATG+TGG 0.768274 2.3:+31919638 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAAAATAAATAAATATGA+GGG - chr2.3:31919507-31919526 MS.gene60894:CDS 10.0%
!! TTAATATAAACCAAAATAAA+AGG + chr2.3:31920269-31920288 None:intergenic 10.0%
!! AAGAATATTTAATAAGCTAT+CGG - chr2.3:31919355-31919374 MS.gene60894:CDS 15.0%
!! ATAAAAATTTCATATATGCA+GGG - chr2.3:31920577-31920596 MS.gene60894:intron 15.0%
!! ATTAAATTAACAAAAAAGTG+AGG - chr2.3:31920423-31920442 MS.gene60894:intron 15.0%
!! ATTAATTAATTAGTTACCAT+TGG - chr2.3:31919868-31919887 MS.gene60894:intron 15.0%
!! ATTTATAGAAAAGAAAAGTT+AGG - chr2.3:31919730-31919749 MS.gene60894:CDS 15.0%
!! CTACAAAATAAATAAATATG+AGG - chr2.3:31919506-31919525 MS.gene60894:CDS 15.0%
!! TACAATTATGATATAACTTA+TGG - chr2.3:31921233-31921252 MS.gene60894:CDS 15.0%
!! TATAAAAATTTCATATATGC+AGG - chr2.3:31920576-31920595 MS.gene60894:intron 15.0%
!! TATTAAAATGAATTCTAAGT+TGG - chr2.3:31920337-31920356 MS.gene60894:intron 15.0%
!!! CACTTTTTTGTTAATTTAAT+TGG + chr2.3:31920423-31920442 None:intergenic 15.0%
!!! CTTTTCTATAAATTAAAGTT+TGG + chr2.3:31919723-31919742 None:intergenic 15.0%
!! AAAATAAATAAATATGAGGG+AGG - chr2.3:31919510-31919529 MS.gene60894:CDS 20.0%
!! AATTTATAAGTACAAGACAT+TGG - chr2.3:31920526-31920545 MS.gene60894:intron 20.0%
!! CTTTAAATTATGATGATCAT+TGG - chr2.3:31920551-31920570 MS.gene60894:intron 20.0%
!! GTTATTATTATATTGTGTTG+TGG + chr2.3:31920217-31920236 None:intergenic 20.0%
!! TAATAGAAGAAGAAGAAAAT+AGG + chr2.3:31919757-31919776 None:intergenic 20.0%
!! TATATTAACATAATCAGACA+TGG - chr2.3:31920281-31920300 MS.gene60894:intron 20.0%
!! TGATGTTAATATGGTTTATA+CGG - chr2.3:31919157-31919176 MS.gene60894:CDS 20.0%
!! TTTCTTCTTCTTCTATTATT+TGG - chr2.3:31919759-31919778 MS.gene60894:CDS 20.0%
!!! CAGATATTTCTATCAAATAA+TGG - chr2.3:31920855-31920874 MS.gene60894:intron 20.0%
!!! TTTAGTATTCTAGAATATGA+TGG + chr2.3:31920039-31920058 None:intergenic 20.0%
! AAATATCTGAATTGTTGGAT+CGG + chr2.3:31920844-31920863 None:intergenic 25.0%
! AACAAATTTCCAGTAAAAGA+AGG + chr2.3:31919907-31919926 None:intergenic 25.0%
! AAGAGTGATTCTAAGTTATA+GGG + chr2.3:31920239-31920258 None:intergenic 25.0%
! AAGTCACATATAATAAGTGA+TGG + chr2.3:31920071-31920090 None:intergenic 25.0%
! AGTAACAATTATAGGAGTTA+TGG - chr2.3:31920307-31920326 MS.gene60894:intron 25.0%
! AGTAATATTCATTAATGGTC+TGG - chr2.3:31919436-31919455 MS.gene60894:CDS 25.0%
! CATGGAAAAGTAACAATTAT+AGG - chr2.3:31920299-31920318 MS.gene60894:intron 25.0%
! GAAATAACATTGTAACCATA+AGG - chr2.3:31920397-31920416 MS.gene60894:intron 25.0%
! GATAGAAATATCTGAATTGT+TGG + chr2.3:31920849-31920868 None:intergenic 25.0%
! GTAATATTCATTAATGGTCT+GGG - chr2.3:31919437-31919456 MS.gene60894:CDS 25.0%
! TGAAAAAAGAAAAGAGAGAA+AGG + chr2.3:31920503-31920522 None:intergenic 25.0%
! TTAAAAGGGTTGTTGAATTT+GGG - chr2.3:31919189-31919208 MS.gene60894:CDS 25.0%
!! AGAATATGATGGAACTTAAA+TGG + chr2.3:31920028-31920047 None:intergenic 25.0%
!! ATAAGTTGAATGTGTTTCAT+TGG - chr2.3:31919063-31919082 MS.gene60894:CDS 25.0%
!!! ATAGTTTGCCATGTTTTAAA+AGG + chr2.3:31921212-31921231 None:intergenic 25.0%
!!! ATCTCTGTTTTTTAAGATGT+TGG - chr2.3:31920098-31920117 MS.gene60894:intron 25.0%
!!! CTTCAAATCTCCTTTTATTT+TGG - chr2.3:31920256-31920275 MS.gene60894:intron 25.0%
!!! TGTTAATTTAATTGGCCTTA+TGG + chr2.3:31920415-31920434 None:intergenic 25.0%
!!! TGTTTTGAAAAAAGTGCTTT+CGG - chr2.3:31919328-31919347 MS.gene60894:CDS 25.0%
AAGAAACTACTATCCAGTAA+AGG - chr2.3:31919007-31919026 MS.gene60894:CDS 30.0%
AAGGTAACTTATCACATGTA+AGG + chr2.3:31919400-31919419 None:intergenic 30.0%
AATTAAAGTTTGGACGAGTT+TGG + chr2.3:31919713-31919732 None:intergenic 30.0%
AATTATAGGAGTTATGGTGT+TGG - chr2.3:31920313-31920332 MS.gene60894:intron 30.0%
ACTAAAAGGATAGTATCTTC+TGG - chr2.3:31921056-31921075 MS.gene60894:intron 30.0%
AGGTGCTTATGATGTTAATA+TGG - chr2.3:31919148-31919167 MS.gene60894:CDS 30.0%
CAACAGATAGATTGAATGAA+TGG - chr2.3:31921426-31921445 MS.gene60894:CDS 30.0%
CGAGCAGTAATATTCATTAA+TGG - chr2.3:31919431-31919450 MS.gene60894:CDS 30.0%
GAAAAGTTAGAACATGTCAA+TGG - chr2.3:31919550-31919569 MS.gene60894:CDS 30.0%
GAAGAGTGATTCTAAGTTAT+AGG + chr2.3:31920240-31920259 None:intergenic 30.0%
GTTAAAAGGGTTGTTGAATT+TGG - chr2.3:31919188-31919207 MS.gene60894:CDS 30.0%
GTTCTTAACAAATCCTTTAC+TGG + chr2.3:31919023-31919042 None:intergenic 30.0%
TTAATTGCTTAATTCAGTGC+TGG + chr2.3:31919853-31919872 None:intergenic 30.0%
! TGTTGTTGATAAATGTCTCA+AGG + chr2.3:31920896-31920915 None:intergenic 30.0%
!! AAAAGTATTCTTTCGAGGAA+TGG - chr2.3:31919460-31919479 MS.gene60894:CDS 30.0%
!! ATACGGTTGATGATGTTAAA+AGG - chr2.3:31919174-31919193 MS.gene60894:CDS 30.0%
!! GGTTACAATGTTATTTCTCA+TGG + chr2.3:31920394-31920413 None:intergenic 30.0%
!! TACGGTTGATGATGTTAAAA+GGG - chr2.3:31919175-31919194 MS.gene60894:CDS 30.0%
!!! GCACTATACTTTTTTCTTGT+GGG - chr2.3:31920154-31920173 MS.gene60894:intron 30.0%
!!! TGCACTATACTTTTTTCTTG+TGG - chr2.3:31920153-31920172 MS.gene60894:intron 30.0%
AACCAATTTCGCTTCTTCTT+CGG + chr2.3:31921264-31921283 None:intergenic 35.0%
AGTATTTACGACAACCAAAC+AGG - chr2.3:31921152-31921171 MS.gene60894:intron 35.0%
ATGGACATGACAACACTAAA+AGG - chr2.3:31921042-31921061 MS.gene60894:intron 35.0%
CAGAATGGTAAAATTGTTCC+GGG + chr2.3:31920788-31920807 None:intergenic 35.0%
CCAGAATGGTAAAATTGTTC+CGG + chr2.3:31920789-31920808 None:intergenic 35.0%
CGAATCCAAAACAGTTTCAT+CGG + chr2.3:31921321-31921340 None:intergenic 35.0%
GACAAAAAACAGAACTAACC+AGG + chr2.3:31919254-31919273 None:intergenic 35.0%
GACTTCTATTATCTTGACTG+TGG - chr2.3:31921104-31921123 MS.gene60894:intron 35.0%
GGTTGAGGGATAAAATGAAA+GGG + chr2.3:31920923-31920942 None:intergenic 35.0%
TAACATGTGCTAACATGTTC+TGG - chr2.3:31920637-31920656 MS.gene60894:intron 35.0%
TAGCACATGTTACAATGTCA+GGG + chr2.3:31920629-31920648 None:intergenic 35.0%
TATTATCTTGACTGTGGACA+CGG - chr2.3:31921110-31921129 MS.gene60894:intron 35.0%
TCTTTAGTGAGAATCGTTGA+TGG + chr2.3:31920996-31921015 None:intergenic 35.0%
TGATAAATGTCTCAAGGACT+TGG + chr2.3:31920890-31920909 None:intergenic 35.0%
TGTATTTGTCTAGCACTTTG+CGG - chr2.3:31919795-31919814 MS.gene60894:CDS 35.0%
TGTGATTAGAGAGTGATGAA+AGG - chr2.3:31919304-31919323 MS.gene60894:CDS 35.0%
TTACATATTCCTCACAAACG+AGG + chr2.3:31919630-31919649 None:intergenic 35.0%
TTAGCACATGTTACAATGTC+AGG + chr2.3:31920630-31920649 None:intergenic 35.0%
! AACATGTGATTTTGCAGACA+TGG - chr2.3:31921015-31921034 MS.gene60894:intron 35.0%
! ACTGCTCGAAAGATGTTTTA+AGG + chr2.3:31919419-31919438 None:intergenic 35.0%
! ATTTTGCAGACATGGAACAA+TGG - chr2.3:31921023-31921042 MS.gene60894:intron 35.0%
!! ATCACATGTAAGGCTTTGTT+TGG + chr2.3:31919390-31919409 None:intergenic 35.0%
!! GATGTTGGTTTCTGAAATGT+TGG - chr2.3:31920113-31920132 MS.gene60894:intron 35.0%
!! TTTGAAAAAAGTGCTTTCGG+TGG - chr2.3:31919331-31919350 MS.gene60894:CDS 35.0%
!!! AAGCGAAATTGGTTTTAGGT+GGG - chr2.3:31921270-31921289 MS.gene60894:CDS 35.0%
!!! GAAGAAGCGAAATTGGTTTT+AGG - chr2.3:31921266-31921285 MS.gene60894:CDS 35.0%
AAATTGTTCCGGGAACAATG+TGG + chr2.3:31920778-31920797 None:intergenic 40.0%
AATAGACAACAGTACGGTTG+AGG + chr2.3:31920938-31920957 None:intergenic 40.0%
ACACACCAAAGTGGTTGAAT+AGG + chr2.3:31921488-31921507 None:intergenic 40.0%
ACAGGAAGTGACAAAAACGA+TGG - chr2.3:31921170-31921189 MS.gene60894:intron 40.0%
ACATGTTACAATGTCAGGGT+AGG + chr2.3:31920625-31920644 None:intergenic 40.0%
AGAAACATGCGGATAGAAAC+AGG + chr2.3:31919936-31919955 None:intergenic 40.0%
AGGCAGAGGAATAATACCAA+TGG + chr2.3:31919887-31919906 None:intergenic 40.0%
ATAGACAACAGTACGGTTGA+GGG + chr2.3:31920937-31920956 None:intergenic 40.0%
CAACCGTACTGTTGTCTATT+GGG - chr2.3:31920938-31920957 MS.gene60894:intron 40.0%
CGGTTGAGGGATAAAATGAA+AGG + chr2.3:31920924-31920943 None:intergenic 40.0%
CTTCCCAATAGACAACAGTA+CGG + chr2.3:31920944-31920963 None:intergenic 40.0%
GAATGAATGGAGAAGCAGAA+TGG - chr2.3:31921439-31921458 MS.gene60894:CDS 40.0%
GGATTTAAATGACCTGTTCC+CGG + chr2.3:31920705-31920724 None:intergenic 40.0%
GGTTCAGAAGGAAGAATCAA+AGG + chr2.3:31919113-31919132 None:intergenic 40.0%
TCAACCGTACTGTTGTCTAT+TGG - chr2.3:31920937-31920956 MS.gene60894:intron 40.0%
TCAGGCAATAGGGATGAAAA+GGG - chr2.3:31921383-31921402 MS.gene60894:CDS 40.0%
TCTTCCTTCTGAACCAATGT+TGG - chr2.3:31919118-31919137 MS.gene60894:CDS 40.0%
TCTTTGACCTGAGAAACATG+CGG + chr2.3:31919947-31919966 None:intergenic 40.0%
TTCATATATGCAGGGCATAC+AGG - chr2.3:31920585-31920604 MS.gene60894:intron 40.0%
TTTCCAGTAAAAGAAGGCAG+AGG + chr2.3:31919901-31919920 None:intergenic 40.0%
TTTCTATCCGCATGTTTCTC+AGG - chr2.3:31919937-31919956 MS.gene60894:intron 40.0%
! ACTAGCCGATGAAACTGTTT+TGG - chr2.3:31921313-31921332 MS.gene60894:CDS 40.0%
! AGAATGGTGAGTAGAGGTAT+AGG - chr2.3:31921455-31921474 MS.gene60894:CDS 40.0%
! ATTCCTCTGCCTTCTTTTAC+TGG - chr2.3:31919895-31919914 MS.gene60894:intron 40.0%
! CCGGAACAATTTTACCATTC+TGG - chr2.3:31920786-31920805 MS.gene60894:intron 40.0%
! TGAGAATCGTTGATGGAACA+TGG + chr2.3:31920989-31921008 None:intergenic 40.0%
! TGGCAAAGCATAAAGCATCT+TGG - chr2.3:31920357-31920376 MS.gene60894:intron 40.0%
! TTCAACCACTTTGGTGTGTT+AGG - chr2.3:31921489-31921508 MS.gene60894:CDS 40.0%
!! CGTTTTTGTCACTTCCTGTT+TGG + chr2.3:31921169-31921188 None:intergenic 40.0%
!! CTGGGAAAAGTATTCTTTCG+AGG - chr2.3:31919455-31919474 MS.gene60894:CDS 40.0%
!!! AGCGAAATTGGTTTTAGGTG+GGG - chr2.3:31921271-31921290 MS.gene60894:CDS 40.0%
!!! GAAGCGAAATTGGTTTTAGG+TGG - chr2.3:31921269-31921288 MS.gene60894:CDS 40.0%
!!! GGTGTGGACCTTTTAAAACA+TGG - chr2.3:31921201-31921220 MS.gene60894:CDS 40.0%
AAACGTTCTCACAGCTTGCA+TGG - chr2.3:31918901-31918920 MS.gene60894:CDS 45.0%
AACGTTCTCACAGCTTGCAT+GGG - chr2.3:31918902-31918921 MS.gene60894:CDS 45.0%
AGAAGCAGAATGGTGAGTAG+AGG - chr2.3:31921449-31921468 MS.gene60894:CDS 45.0%
CATGTGCTAACATGTTCTGG+TGG - chr2.3:31920640-31920659 MS.gene60894:intron 45.0%
CGATGTAACCACATTGTTCC+CGG - chr2.3:31920767-31920786 MS.gene60894:intron 45.0%
CTGAGCCTATTCAACCACTT+TGG - chr2.3:31921480-31921499 MS.gene60894:CDS 45.0%
GACCGAAGAAGAAGCGAAAT+TGG - chr2.3:31921259-31921278 MS.gene60894:CDS 45.0%
GGAAGTGACAAAAACGATGG+CGG - chr2.3:31921173-31921192 MS.gene60894:intron 45.0%
GTACTGTGTTGCACATACCA+GGG + chr2.3:31921517-31921536 None:intergenic 45.0%
GTATGACATTCTGCAGGACT+TGG + chr2.3:31920746-31920765 None:intergenic 45.0%
GTATGAGAACCTCGTTTGTG+AGG - chr2.3:31919618-31919637 MS.gene60894:CDS 45.0%
GTCAGGCAATAGGGATGAAA+AGG - chr2.3:31921382-31921401 MS.gene60894:CDS 45.0%
GTCTCTCACTACCAATTGGT+TGG + chr2.3:31920184-31920203 None:intergenic 45.0%
GTGTGTCTCTCACTACCAAT+TGG + chr2.3:31920188-31920207 None:intergenic 45.0%
GTTGAATTTGGGCTTGATCG+TGG - chr2.3:31919200-31919219 MS.gene60894:CDS 45.0%
TACAATGTCAGGGTAGGCTA+AGG + chr2.3:31920619-31920638 None:intergenic 45.0%
TAGAGTCAGGAAAAGCTTCG+AGG + chr2.3:31919677-31919696 None:intergenic 45.0%
TCAGCCATTGCAGAAGTTCT+AGG + chr2.3:31921353-31921372 None:intergenic 45.0%
TGGTAGGTCTTGGGGTTTAA+GGG + chr2.3:31920726-31920745 None:intergenic 45.0%
TGTACTGTGTTGCACATACC+AGG + chr2.3:31921518-31921537 None:intergenic 45.0%
TTGGCCTAGAACTTCTGCAA+TGG - chr2.3:31921346-31921365 MS.gene60894:CDS 45.0%
TTGGTAGGTCTTGGGGTTTA+AGG + chr2.3:31920727-31920746 None:intergenic 45.0%
! GAACCAATGTTGGCTGAGAA+AGG - chr2.3:31919128-31919147 MS.gene60894:CDS 45.0%
! GAAGTCTGATGATGACACGA+TGG + chr2.3:31921090-31921109 None:intergenic 45.0%
! GCATAAAGCATCTTGGACTG+TGG - chr2.3:31920364-31920383 MS.gene60894:intron 45.0%
!! CTGTTTTGGATTCGCGACTT+TGG - chr2.3:31921327-31921346 MS.gene60894:CDS 45.0%
!! TAACCAGGAGCATCAATCTC+AGG + chr2.3:31919239-31919258 None:intergenic 45.0%
!! TCAGCCAACATTGGTTCAGA+AGG + chr2.3:31919125-31919144 None:intergenic 45.0%
!! TTGTGGGAGTACCAACCAAT+TGG - chr2.3:31920170-31920189 MS.gene60894:intron 45.0%
!!! GAAATTGGTTTTAGGTGGGG+AGG - chr2.3:31921274-31921293 MS.gene60894:CDS 45.0%
ACACCGCGATGACCAAACAA+CGG + chr2.3:31918972-31918991 None:intergenic 50.0%
ACGTTCTCACAGCTTGCATG+GGG - chr2.3:31918903-31918922 MS.gene60894:CDS 50.0%
AGATCGTCTTGCAACAGAGC+CGG - chr2.3:31920683-31920702 MS.gene60894:intron 50.0%
AGTCCTTGTGGTCAGGCAAT+AGG - chr2.3:31921372-31921391 MS.gene60894:CDS 50.0%
ATCCCTATTGCCTGACCACA+AGG + chr2.3:31921378-31921397 None:intergenic 50.0%
ATGCAGGGCATACAGGATCA+TGG - chr2.3:31920592-31920611 MS.gene60894:intron 50.0%
CATCGCGTATGACATTCTGC+AGG + chr2.3:31920752-31920771 None:intergenic 50.0%
GCACCTTTCTCAGCCAACAT+TGG + chr2.3:31919134-31919153 None:intergenic 50.0%
GGGTTCCTAACACACCAAAG+TGG + chr2.3:31921497-31921516 None:intergenic 50.0%
GTCCTTGTGGTCAGGCAATA+GGG - chr2.3:31921373-31921392 MS.gene60894:CDS 50.0%
TCCTGACTCTAGTGAGCTCT+GGG - chr2.3:31919686-31919705 MS.gene60894:CDS 50.0%
TGCAGGGCATACAGGATCAT+GGG - chr2.3:31920593-31920612 MS.gene60894:intron 50.0%
TTCCTGACTCTAGTGAGCTC+TGG - chr2.3:31919685-31919704 MS.gene60894:CDS 50.0%
TTGCCTGAGATTGATGCTCC+TGG - chr2.3:31919233-31919252 MS.gene60894:CDS 50.0%
! ACAAAAACGATGGCGGCTCT+TGG - chr2.3:31921180-31921199 MS.gene60894:intron 50.0%
! CTTTGGTGTGTTAGGAACCC+TGG - chr2.3:31921497-31921516 MS.gene60894:CDS 50.0%
! GACATTCTGCAGGACTTGGT+AGG + chr2.3:31920742-31920761 None:intergenic 50.0%
! TGCAGGACTTGGTAGGTCTT+GGG + chr2.3:31920735-31920754 None:intergenic 50.0%
AACATGTTCTGGTGGCCAGC+CGG - chr2.3:31920648-31920667 MS.gene60894:intron 55.0%
ACGATCTGGCCAATCGCTTC+CGG + chr2.3:31920670-31920689 None:intergenic 55.0%
ATGACGTCGTACCAGGTTGC+TGG - chr2.3:31920814-31920833 MS.gene60894:intron 55.0%
ATGGCTGAGTCCTTGTGGTC+AGG - chr2.3:31921365-31921384 MS.gene60894:CDS 55.0%
CGACGTCATCAGCACCAGAA+TGG + chr2.3:31920803-31920822 None:intergenic 55.0%
CTGCAATGGCTGAGTCCTTG+TGG - chr2.3:31921360-31921379 MS.gene60894:CDS 55.0%
GATCGTCTTGCAACAGAGCC+GGG - chr2.3:31920684-31920703 MS.gene60894:intron 55.0%
GGCTCTGTTGCAAGACGATC+TGG + chr2.3:31920684-31920703 None:intergenic 55.0%
GTCAATGACGCGCCGTTGTT+TGG - chr2.3:31918957-31918976 MS.gene60894:CDS 55.0%
TCCCAGAGCTCACTAGAGTC+AGG + chr2.3:31919690-31919709 None:intergenic 55.0%
TGTGGACACGGCGACTTTAC+TGG - chr2.3:31921122-31921141 MS.gene60894:intron 55.0%
! CTGATGATGACACGATGGCG+CGG + chr2.3:31921085-31921104 None:intergenic 55.0%
! CTGCAGGACTTGGTAGGTCT+TGG + chr2.3:31920736-31920755 None:intergenic 55.0%
! GCAGGACTTGGTAGGTCTTG+GGG + chr2.3:31920734-31920753 None:intergenic 55.0%
!! GGATCGGTTTTCCAGCAACC+TGG + chr2.3:31920828-31920847 None:intergenic 55.0%
CTTGCAACAGAGCCGGGAAC+AGG - chr2.3:31920690-31920709 MS.gene60894:intron 60.0%
GCGCCGTTGTTTGGTCATCG+CGG - chr2.3:31918966-31918985 MS.gene60894:CDS 60.0%
TAGGTGGGGAGGTAGCGTTG+TGG - chr2.3:31921285-31921304 MS.gene60894:CDS 60.0%
! AACGATGGCGGCTCTTGGTG+TGG - chr2.3:31921185-31921204 MS.gene60894:CDS 60.0%
! GGGTCTGAAGCGATACGCAG+AGG - chr2.3:31921403-31921422 MS.gene60894:CDS 60.0%
!! GGTGCTGATGACGTCGTACC+AGG - chr2.3:31920807-31920826 MS.gene60894:intron 60.0%
ACGTTCACAGCCACGCGCGT+TGG + chr2.3:31918933-31918952 None:intergenic 65.0%
ATGGGGAAACCCAACGCGCG+TGG - chr2.3:31918920-31918939 MS.gene60894:CDS 65.0%
CGTTCACAGCCACGCGCGTT+GGG + chr2.3:31918932-31918951 None:intergenic 65.0%
TCTGGCCAATCGCTTCCGGC+TGG + chr2.3:31920666-31920685 None:intergenic 65.0%
GGTGGCCAGCCGGAAGCGAT+TGG - chr2.3:31920658-31920677 MS.gene60894:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 31918888 31921547 31918888 ID=MS.gene60894
chr2.3 mRNA 31918888 31921547 31918888 ID=MS.gene60894.t1;Parent=MS.gene60894
chr2.3 exon 31921181 31921547 31921181 ID=MS.gene60894.t1.exon1;Parent=MS.gene60894.t1
chr2.3 CDS 31921181 31921547 31921181 ID=cds.MS.gene60894.t1;Parent=MS.gene60894.t1
chr2.3 exon 31918888 31919837 31918888 ID=MS.gene60894.t1.exon2;Parent=MS.gene60894.t1
chr2.3 CDS 31918888 31919837 31918888 ID=cds.MS.gene60894.t1;Parent=MS.gene60894.t1
Gene Sequence

>MS.gene60894

ATGCATGGTTTAGAAACGTTCTCACAGCTTGCATGGGGAAACCCAACGCGCGTGGCTGTGAACGTGCGTGTCAATGACGCGCCGTTGTTTGGTCATCGCGGTGTGATGTTAGATGTTTCAAGAAACTACTATCCAGTAAAGGATTTGTTAAGAACTATTGAAGCTATGAGTATGAATAAGTTGAATGTGTTTCATTGGCATGTAACAGATTCTCACTCTTTTCCTTTGATTCTTCCTTCTGAACCAATGTTGGCTGAGAAAGGTGCTTATGATGTTAATATGGTTTATACGGTTGATGATGTTAAAAGGGTTGTTGAATTTGGGCTTGATCGTGGTGTTCGTGTTTTGCCTGAGATTGATGCTCCTGGTTAGTTCTGTTTTTTGTCTTTAATTTGTTGATTTACTTTACTAAATTGTGTGATTAGAGAGTGATGAAAGGATGTTTTGAAAAAAGTGCTTTCGGTGGCAAGAATATTTAATAAGCTATCGGCTATTTTTTCCAAACAAAGCCTTACATGTGATAAGTTACCTTAAAACATCTTTCGAGCAGTAATATTCATTAATGGTCTGGGAAAAGTATTCTTTCGAGGAATGGTATGTTGAGAATAATCTGATTTACTACAAAATAAATAAATATGAGGGAGGATAAGTACTCTTTTCAAGAAAAGTTAGAACATGTCAATGGTTCAGCTCAAATTGTACAACAACTTGAGTTCGAATTCTGTTACTTGTATGAGAACCTCGTTTGTGAGGAATATGTAAGTAACTTCGTTAGCGCTCATTGAGCCTCGAAGCTTTTCCTGACTCTAGTGAGCTCTGGGACCAAACTCGTCCAAACTTTAATTTATAGAAAAGAAAAGTTAGGACCTATTTTCTTCTTCTTCTATTATTTGGACTTTTGTTGTTTTGTATTTGTCTAGCACTTTGCGGCACAATTGTAATGAAGATTGACACTGTAAAGACCAGCACTGAATTAAGCAATTAATTAATTAGTTACCATTGGTATTATTCCTCTGCCTTCTTTTACTGGAAATTTGTTATTATGCCTGTTTCTATCCGCATGTTTCTCAGGTCAAAGAATTTATCTAGATTTGTGTGATTAACTATACTGATTATCTAATTAGTTAGTGTTAACTTCCATTTAAGTTCCATCATATTCTAGAATACTAAACTTGTGTCGCCATCACTTATTATATGTGACTTTAGAAACATCTCTGTTTTTTAAGATGTTGGTTTCTGAAATGTTGGAATCTGTTTATCTATAATGCACTATACTTTTTTCTTGTGGGAGTACCAACCAATTGGTAGTGAGAGACACACACTTTACCACAACACAATATAATAATAACCCTATAACTTAGAATCACTCTTCAAATCTCCTTTTATTTTGGTTTATATTAACATAATCAGACATGGAAAAGTAACAATTATAGGAGTTATGGTGTTGGTTATTAAAATGAATTCTAAGTTGGCAAAGCATAAAGCATCTTGGACTGTGGCTTACCATGAGAAATAACATTGTAACCATAAGGCCAATTAAATTAACAAAAAAGTGAGGCTTTAGAATTCTGTTTTTGCTTTTATACTATATTCTGATTCAAAAGCATGCATGCCTTTCTCTCTTTTCTTTTTTCAAATAATTTATAAGTACAAGACATTGGTTCTTTAAATTATGATGATCATTGGCATATAAAAATTTCATATATGCAGGGCATACAGGATCATGGGCCTTAGCCTACCCTGACATTGTAACATGTGCTAACATGTTCTGGTGGCCAGCCGGAAGCGATTGGCCAGATCGTCTTGCAACAGAGCCGGGAACAGGTCATTTAAATCCCTTAAACCCCAAGACCTACCAAGTCCTGCAGAATGTCATACGCGATGTAACCACATTGTTCCCGGAACAATTTTACCATTCTGGTGCTGATGACGTCGTACCAGGTTGCTGGAAAACCGATCCAACAATTCAGATATTTCTATCAAATAATGGAACTCTCAGCCAAGTCCTTGAGACATTTATCAACAACACTCTCCCTTTCATTTTATCCCTCAACCGTACTGTTGTCTATTGGGAAGATGTTCTATTAGACGATACAGTCCATGTTCCATCAACGATTCTCACTAAAGAACATGTGATTTTGCAGACATGGAACAATGGACATGACAACACTAAAAGGATAGTATCTTCTGGATACCGCGCCATCGTGTCATCATCAGACTTCTATTATCTTGACTGTGGACACGGCGACTTTACTGGAAATAACAGTATTTACGACAACCAAACAGGAAGTGACAAAAACGATGGCGGCTCTTGGTGTGGACCTTTTAAAACATGGCAAACTATATACAATTATGATATAACTTATGGACTGACCGAAGAAGAAGCGAAATTGGTTTTAGGTGGGGAGGTAGCGTTGTGGTCAGAACTAGCCGATGAAACTGTTTTGGATTCGCGACTTTGGCCTAGAACTTCTGCAATGGCTGAGTCCTTGTGGTCAGGCAATAGGGATGAAAAGGGTCTGAAGCGATACGCAGAGGCAACAGATAGATTGAATGAATGGAGAAGCAGAATGGTGAGTAGAGGTATAGGAGCTGAGCCTATTCAACCACTTTGGTGTGTTAGGAACCCTGGTATGTGCAACACAGTACATGCCATTTAG
Protein sequence

>MS.gene60894.t1

MHGLETFSQLAWGNPTRVAVNVRVNDAPLFGHRGVMLDVSRNYYPVKDLLRTIEAMSMNKLNVFHWHVTDSHSFPLILPSEPMLAEKGAYDVNMVYTVDDVKRVVEFGLDRGVRVLPEIDAPGHTGSWALAYPDIVTCANMFWWPAGSDWPDRLATEPGTGHLNPLNPKTYQVLQNVIRDVTTLFPEQFYHSGADDVVPGCWKTDPTIQIFLSNNGTLSQVLETFINNTLPFILSLNRTVVYWEDVLLDDTVHVPSTILTKEHVILQTWNNGHDNTKRIVSSGYRAIVSSSDFYYLDCGHGDFTGNNSIYDNQTGSDKNDGGSWCGPFKTWQTIYNYDITYGLTEEEAKLVLGGEVALWSELADETVLDSRLWPRTSAMAESLWSGNRDEKGLKRYAEATDRLNEWRSRMVSRGIGAEPIQPLWCVRNPGMCNTVHAI