Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60981.t1 | XP_003628802.1 | 67.9 | 53 | 10 | 1 | 1 | 46 | 1 | 53 | 3.70E-06 | 61.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60981.t1 | G7LHP4 | 67.9 | 53 | 10 | 1 | 1 | 46 | 1 | 53 | 2.6e-06 | 61.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene60981 | 0.841476 | 4.64E-58 | -1.69E-46 |
| MS.gene054184 | MS.gene60981 | 0.803393 | 3.56E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene60981 | 0.803931 | 2.75E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60981.t1 | MTR_8g066920 | 58.667 | 75 | 16 | 2 | 1 | 68 | 1 | 67 | 7.79e-14 | 64.3 |
| MS.gene60981.t1 | MTR_8g067020 | 100.000 | 26 | 0 | 0 | 1 | 26 | 18 | 43 | 4.84e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGTGGTGGTGGACTTCC+TGG | 0.215833 | 8.1:-37474073 | MS.gene60981:CDS |
| CCTATTGTTGGTCCAATTCT+TGG | 0.231596 | 8.1:-37474004 | MS.gene60981:CDS |
| CTTGGTGGTGGTGGACTTCC+TGG | 0.233905 | 8.1:-37474154 | MS.gene60981:CDS |
| CCTGGTGGTGGTGGACTTCC+TGG | 0.234627 | 8.1:-37474187 | MS.gene60981:CDS |
| CATTGCTCTCTTTGTGCTGT+TGG | 0.329983 | 8.1:-37474311 | MS.gene60981:CDS |
| CCTGGCGGCAGTGGACTTCC+TGG | 0.338892 | 8.1:-37474109 | MS.gene60981:CDS |
| GGACTGTCTGGTGGTGGTGG+TGG | 0.376472 | 8.1:-37474082 | MS.gene60981:CDS |
| CGGCACTATCCACCAAGAAT+TGG | 0.380603 | 8.1:+37473992 | None:intergenic |
| ATTGCTCTCTTTGTGCTGTT+GGG | 0.382762 | 8.1:-37474310 | MS.gene60981:CDS |
| CTTCCTGGTGGAGGACTGTC+TGG | 0.388078 | 8.1:-37474094 | MS.gene60981:CDS |
| GGAGGACTGCCTGGCGGCAG+TGG | 0.390560 | 8.1:-37474118 | MS.gene60981:CDS |
| GGAGGACTGTCTGGTGGTGG+TGG | 0.397145 | 8.1:-37474085 | MS.gene60981:CDS |
| GAAGGATCTGGAGGATTTCC+TGG | 0.399251 | 8.1:-37474220 | MS.gene60981:CDS |
| CCAAGAATTGGACCAACAAT+AGG | 0.421284 | 8.1:+37474004 | None:intergenic |
| GGACTTCCTGGTGTAGGATC+TGG | 0.425340 | 8.1:-37474142 | MS.gene60981:CDS |
| GGTGGAGGACTGTCTGGTGG+TGG | 0.426939 | 8.1:-37474088 | MS.gene60981:CDS |
| AGAGGACTGCTTGGTGGTGG+TGG | 0.438325 | 8.1:-37474163 | MS.gene60981:CDS |
| GGATCTGGTGGAGGACTGCC+TGG | 0.440862 | 8.1:-37474127 | MS.gene60981:CDS |
| GGACTTCCTGGTGTAGGATC+TGG | 0.450507 | 8.1:-37474061 | MS.gene60981:CDS |
| CTCGGAGGACTGCCTATTGT+TGG | 0.453065 | 8.1:-37474016 | MS.gene60981:CDS |
| TGACAATGGTGATTCTGATC+CGG | 0.460184 | 8.1:-37474254 | MS.gene60981:CDS |
| ATTGTTGGTCCAATTCTTGG+TGG | 0.461856 | 8.1:-37474001 | MS.gene60981:CDS |
| GATCCTTCTTCACTAGGAAC+CGG | 0.490401 | 8.1:+37474235 | None:intergenic |
| GGTGGTGGACTTCCTGGTGT+AGG | 0.497549 | 8.1:-37474067 | MS.gene60981:CDS |
| GGTGGTGGACTTCCTGGTGT+AGG | 0.497549 | 8.1:-37474148 | MS.gene60981:CDS |
| CCTCCAGATCCTTCTTCACT+AGG | 0.502330 | 8.1:+37474229 | None:intergenic |
| GGTAGAGGACTGCTTGGTGG+TGG | 0.516172 | 8.1:-37474166 | MS.gene60981:CDS |
| GGCAGTGGACTTCCTGGTGG+AGG | 0.517199 | 8.1:-37474103 | MS.gene60981:CDS |
| GATCTGGAGGATTTCCTGGT+GGG | 0.518149 | 8.1:-37474216 | MS.gene60981:CDS |
| GGTGGTGGACTTCCTGGTAG+AGG | 0.519245 | 8.1:-37474181 | MS.gene60981:CDS |
| GTTCCTAGTGAAGAAGGATC+TGG | 0.520941 | 8.1:-37474232 | MS.gene60981:CDS |
| CCAGGAAGTCCACTGCCGCC+AGG | 0.521907 | 8.1:+37474109 | None:intergenic |
| CTTCCTGGTGTAGGATCTGG+TGG | 0.527616 | 8.1:-37474139 | MS.gene60981:CDS |
| CCTGGTAGAGGACTGCTTGG+TGG | 0.533777 | 8.1:-37474169 | MS.gene60981:CDS |
| TCTGGAGGATTTCCTGGTGG+GGG | 0.534146 | 8.1:-37474214 | MS.gene60981:CDS |
| ATCTGGAGGATTTCCTGGTG+GGG | 0.538112 | 8.1:-37474215 | MS.gene60981:CDS |
| CCTCTACCAGATCCTACACC+AGG | 0.549251 | 8.1:+37474055 | None:intergenic |
| GATCCGGTTCCTAGTGAAGA+AGG | 0.551329 | 8.1:-37474238 | MS.gene60981:CDS |
| CCTCCACCAGATCCTACACC+AGG | 0.551423 | 8.1:+37474136 | None:intergenic |
| CCACCAAGCAGTCCTCTACC+AGG | 0.560853 | 8.1:+37474169 | None:intergenic |
| ACTAGAAAACTCTTTGACAA+TGG | 0.565706 | 8.1:-37474268 | MS.gene60981:CDS |
| CTTCCTGGTAGAGGACTGCT+TGG | 0.576335 | 8.1:-37474172 | MS.gene60981:CDS |
| CCACCAGACAGTCCTCCACC+AGG | 0.586175 | 8.1:+37474091 | None:intergenic |
| CCAGGAAGTCCACCACCACC+AGG | 0.587068 | 8.1:+37474187 | None:intergenic |
| CCTGGTGTAGGATCTGGTAG+AGG | 0.595064 | 8.1:-37474055 | MS.gene60981:CDS |
| GGCGGCAGTGGACTTCCTGG+TGG | 0.598551 | 8.1:-37474106 | MS.gene60981:CDS |
| GGACTGCTCGAAGGACTGCT+CGG | 0.599291 | 8.1:-37474034 | MS.gene60981:CDS |
| CCTGGTGGAGGACTGTCTGG+TGG | 0.599542 | 8.1:-37474091 | MS.gene60981:CDS |
| CCTGGTGTAGGATCTGGTGG+AGG | 0.602966 | 8.1:-37474136 | MS.gene60981:CDS |
| TCTGGTAGAGGACTGCTCGA+AGG | 0.644242 | 8.1:-37474043 | MS.gene60981:CDS |
| CCTAGTGAAGAAGGATCTGG+AGG | 0.656597 | 8.1:-37474229 | MS.gene60981:CDS |
| GGATCTGGAGGATTTCCTGG+TGG | 0.681748 | 8.1:-37474217 | MS.gene60981:CDS |
| TCTGGTGGAGGACTGCCTGG+CGG | 0.723995 | 8.1:-37474124 | MS.gene60981:CDS |
| CTGCTCGAAGGACTGCTCGG+AGG | 0.730140 | 8.1:-37474031 | MS.gene60981:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTAGAAAACTCTTTGACAA+TGG | - | chr8.1:37474063-37474082 | MS.gene60981:CDS | 30.0% | |
| ATTGTTGGTCCAATTCTTGG+TGG | - | chr8.1:37474330-37474349 | MS.gene60981:CDS | 40.0% | |
| CCAAGAATTGGACCAACAAT+AGG | + | chr8.1:37474330-37474349 | None:intergenic | 40.0% | |
| CCTATTGTTGGTCCAATTCT+TGG | - | chr8.1:37474327-37474346 | MS.gene60981:CDS | 40.0% | |
| TGACAATGGTGATTCTGATC+CGG | - | chr8.1:37474077-37474096 | MS.gene60981:CDS | 40.0% | |
| ! | ATTGCTCTCTTTGTGCTGTT+GGG | - | chr8.1:37474021-37474040 | MS.gene60981:CDS | 40.0% |
| CATTGCTCTCTTTGTGCTGT+TGG | - | chr8.1:37474020-37474039 | MS.gene60981:CDS | 45.0% | |
| GATCCTTCTTCACTAGGAAC+CGG | + | chr8.1:37474099-37474118 | None:intergenic | 45.0% | |
| GTTCCTAGTGAAGAAGGATC+TGG | - | chr8.1:37474099-37474118 | MS.gene60981:CDS | 45.0% | |
| ATCTGGAGGATTTCCTGGTG+GGG | - | chr8.1:37474116-37474135 | MS.gene60981:CDS | 50.0% | |
| CCTAGTGAAGAAGGATCTGG+AGG | - | chr8.1:37474102-37474121 | MS.gene60981:CDS | 50.0% | |
| CCTCCAGATCCTTCTTCACT+AGG | + | chr8.1:37474105-37474124 | None:intergenic | 50.0% | |
| GAAGGATCTGGAGGATTTCC+TGG | - | chr8.1:37474111-37474130 | MS.gene60981:CDS | 50.0% | |
| GATCCGGTTCCTAGTGAAGA+AGG | - | chr8.1:37474093-37474112 | MS.gene60981:CDS | 50.0% | |
| GATCTGGAGGATTTCCTGGT+GGG | - | chr8.1:37474115-37474134 | MS.gene60981:CDS | 50.0% | |
| CCTCTACCAGATCCTACACC+AGG | + | chr8.1:37474279-37474298 | None:intergenic | 55.0% | |
| CCTGGTGTAGGATCTGGTAG+AGG | - | chr8.1:37474276-37474295 | MS.gene60981:CDS | 55.0% | |
| CTCGGAGGACTGCCTATTGT+TGG | - | chr8.1:37474315-37474334 | MS.gene60981:CDS | 55.0% | |
| CTTCCTGGTAGAGGACTGCT+TGG | - | chr8.1:37474159-37474178 | MS.gene60981:CDS | 55.0% | |
| CTTCCTGGTGTAGGATCTGG+TGG | - | chr8.1:37474192-37474211 | MS.gene60981:CDS | 55.0% | |
| GGACTTCCTGGTGTAGGATC+TGG | - | chr8.1:37474189-37474208 | MS.gene60981:CDS | 55.0% | |
| GGATCTGGAGGATTTCCTGG+TGG | - | chr8.1:37474114-37474133 | MS.gene60981:CDS | 55.0% | |
| TCTGGAGGATTTCCTGGTGG+GGG | - | chr8.1:37474117-37474136 | MS.gene60981:CDS | 55.0% | |
| TCTGGTAGAGGACTGCTCGA+AGG | - | chr8.1:37474288-37474307 | MS.gene60981:CDS | 55.0% | |
| CCACCAAGCAGTCCTCTACC+AGG | + | chr8.1:37474165-37474184 | None:intergenic | 60.0% | |
| CCTCCACCAGATCCTACACC+AGG | + | chr8.1:37474198-37474217 | None:intergenic | 60.0% | |
| CCTGGTAGAGGACTGCTTGG+TGG | - | chr8.1:37474162-37474181 | MS.gene60981:CDS | 60.0% | |
| CCTGGTGTAGGATCTGGTGG+AGG | - | chr8.1:37474195-37474214 | MS.gene60981:CDS | 60.0% | |
| CTTCCTGGTGGAGGACTGTC+TGG | - | chr8.1:37474237-37474256 | MS.gene60981:CDS | 60.0% | |
| GGACTGCTCGAAGGACTGCT+CGG | - | chr8.1:37474297-37474316 | MS.gene60981:CDS | 60.0% | |
| ! | GGTAGAGGACTGCTTGGTGG+TGG | - | chr8.1:37474165-37474184 | MS.gene60981:CDS | 60.0% |
| !! | AGAGGACTGCTTGGTGGTGG+TGG | - | chr8.1:37474168-37474187 | MS.gene60981:CDS | 60.0% |
| !! | CTTGGTGGTGGTGGACTTCC+TGG | - | chr8.1:37474177-37474196 | MS.gene60981:CDS | 60.0% |
| !! | GGTGGTGGACTTCCTGGTAG+AGG | - | chr8.1:37474150-37474169 | MS.gene60981:CDS | 60.0% |
| !! | GGTGGTGGACTTCCTGGTGT+AGG | - | chr8.1:37474183-37474202 | MS.gene60981:CDS | 60.0% |
| CCACCAGACAGTCCTCCACC+AGG | + | chr8.1:37474243-37474262 | None:intergenic | 65.0% | |
| CCAGGAAGTCCACCACCACC+AGG | + | chr8.1:37474147-37474166 | None:intergenic | 65.0% | |
| CCTGGTGGAGGACTGTCTGG+TGG | - | chr8.1:37474240-37474259 | MS.gene60981:CDS | 65.0% | |
| CTGCTCGAAGGACTGCTCGG+AGG | - | chr8.1:37474300-37474319 | MS.gene60981:CDS | 65.0% | |
| GGATCTGGTGGAGGACTGCC+TGG | - | chr8.1:37474204-37474223 | MS.gene60981:CDS | 65.0% | |
| GGTGGAGGACTGTCTGGTGG+TGG | - | chr8.1:37474243-37474262 | MS.gene60981:CDS | 65.0% | |
| TCTGGTGGAGGACTGCCTGG+CGG | - | chr8.1:37474207-37474226 | MS.gene60981:CDS | 65.0% | |
| TTTCCTGGTGGGGGACTGCC+TGG | - | chr8.1:37474126-37474145 | MS.gene60981:CDS | 65.0% | |
| ! | GGCAGTGGACTTCCTGGTGG+AGG | - | chr8.1:37474228-37474247 | MS.gene60981:CDS | 65.0% |
| !! | CCTGGTGGTGGTGGACTTCC+TGG | - | chr8.1:37474144-37474163 | MS.gene60981:CDS | 65.0% |
| !! | GGACTGTCTGGTGGTGGTGG+TGG | - | chr8.1:37474249-37474268 | MS.gene60981:CDS | 65.0% |
| !! | GGAGGACTGTCTGGTGGTGG+TGG | - | chr8.1:37474246-37474265 | MS.gene60981:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGACTTCC+TGG | - | chr8.1:37474258-37474277 | MS.gene60981:CDS | 65.0% |
| CCAGGAAGTCCACTGCCGCC+AGG | + | chr8.1:37474225-37474244 | None:intergenic | 70.0% | |
| ! | CCTGGCGGCAGTGGACTTCC+TGG | - | chr8.1:37474222-37474241 | MS.gene60981:CDS | 70.0% |
| ! | GGCGGCAGTGGACTTCCTGG+TGG | - | chr8.1:37474225-37474244 | MS.gene60981:CDS | 70.0% |
| CCACCAGGCAGTCCCCCACC+AGG | + | chr8.1:37474132-37474151 | None:intergenic | 75.0% | |
| CCTGGTGGGGGACTGCCTGG+TGG | - | chr8.1:37474129-37474148 | MS.gene60981:CDS | 75.0% | |
| GGAGGACTGCCTGGCGGCAG+TGG | - | chr8.1:37474213-37474232 | MS.gene60981:CDS | 75.0% | |
| GGTGGGGGACTGCCTGGTGG+TGG | - | chr8.1:37474132-37474151 | MS.gene60981:CDS | 75.0% | |
| !! | GGGGGACTGCCTGGTGGTGG+TGG | - | chr8.1:37474135-37474154 | MS.gene60981:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 37473997 | 37474356 | 37473997 | ID=MS.gene60981 |
| chr8.1 | mRNA | 37473997 | 37474356 | 37473997 | ID=MS.gene60981.t1;Parent=MS.gene60981 |
| chr8.1 | exon | 37473997 | 37474356 | 37473997 | ID=MS.gene60981.t1.exon1;Parent=MS.gene60981.t1 |
| chr8.1 | CDS | 37473997 | 37474356 | 37473997 | ID=cds.MS.gene60981.t1;Parent=MS.gene60981.t1 |
| Gene Sequence |
| Protein sequence |
