Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60983.t1 | XP_003628802.1 | 67.5 | 77 | 13 | 2 | 1 | 77 | 1 | 65 | 7.20E-13 | 84 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60983.t1 | G7LHP4 | 67.5 | 77 | 13 | 2 | 1 | 77 | 1 | 65 | 5.2e-13 | 84.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene60983 | 0.853864 | 1.80E-61 | -1.69E-46 |
| MS.gene054184 | MS.gene60983 | 0.819259 | 1.28E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene60983 | 0.818246 | 2.17E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene60983 | 0.801008 | 1.10E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60983.t1 | MTR_8g066920 | 64.921 | 191 | 14 | 5 | 1 | 161 | 1 | 168 | 7.24e-44 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 64 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCGTGATTCTGAGGATTT+TGG | 0.194043 | 8.1:-37504243 | MS.gene60983:CDS |
| TTGGGTGATGGAGGACTGCC+TGG | 0.249799 | 8.1:-37504114 | MS.gene60983:CDS |
| ACTGGATCCGGAGGACTTCT+TGG | 0.321037 | 8.1:-37504168 | MS.gene60983:CDS |
| CATTGCTCTCTTTGTGCTGT+TGG | 0.329983 | 8.1:-37504304 | MS.gene60983:CDS |
| GATCCAGTGGTTTGTGGAAC+TGG | 0.374467 | 8.1:+37504183 | None:intergenic |
| ATTGCTCTCTTTGTGCTGTT+GGG | 0.382762 | 8.1:-37504303 | MS.gene60983:CDS |
| TGTACCAGATGAAGGAGATT+TGG | 0.396430 | 8.1:-37504052 | MS.gene60983:CDS |
| GGAGTCCTGCCTGATGAAAA+AGG | 0.398693 | 8.1:-37503874 | MS.gene60983:CDS |
| CCCGTTGGTGCAGAAGTTCC+TGG | 0.413835 | 8.1:-37503955 | MS.gene60983:CDS |
| TGGGTGATGGAGGACTGCCT+GGG | 0.414674 | 8.1:-37504113 | MS.gene60983:CDS |
| TATCCTGGTGGTGTGAATTC+CGG | 0.423300 | 8.1:-37503994 | MS.gene60983:CDS |
| TCTTCCAGACCTCCTTCAAC+TGG | 0.449061 | 8.1:+37503898 | None:intergenic |
| CCTCCGGATCCAGTGGTTTG+TGG | 0.457277 | 8.1:+37504177 | None:intergenic |
| CCAGGAACTTCTGCACCAAC+GGG | 0.458513 | 8.1:+37503955 | None:intergenic |
| GATGGAGGACTGCCTGGTAC+TGG | 0.459909 | 8.1:-37503922 | MS.gene60983:CDS |
| ATAGGATCTGGAGCACTGCC+TGG | 0.462961 | 8.1:-37504087 | MS.gene60983:CDS |
| ACCAGGAACTTCTGCACCAA+CGG | 0.467348 | 8.1:+37503954 | None:intergenic |
| TGGACCAGTTGAAGGAGGTC+TGG | 0.467854 | 8.1:-37503902 | MS.gene60983:CDS |
| TGTACCAGGTGAAGAAGGTT+TGG | 0.467962 | 8.1:-37504133 | MS.gene60983:CDS |
| CCTGGTACTGGACCAGTTGA+AGG | 0.468919 | 8.1:-37503910 | MS.gene60983:CDS |
| GGACTTCTTGGAGGACTGCC+TGG | 0.469067 | 8.1:-37504156 | MS.gene60983:CDS |
| GGAGGACTGCCTGGTGTACC+AGG | 0.469604 | 8.1:-37504147 | MS.gene60983:CDS |
| GGAGGAGATGGAGGACTGCC+TGG | 0.473621 | 8.1:-37503928 | MS.gene60983:CDS |
| GTACCAGATGAAGGAGATTT+GGG | 0.475834 | 8.1:-37504051 | MS.gene60983:CDS |
| TCACCCAAATCTCCTTCATC+TGG | 0.480841 | 8.1:+37504048 | None:intergenic |
| GGTGAAGAAGGTTTGGGTGA+TGG | 0.490635 | 8.1:-37504126 | MS.gene60983:CDS |
| AAAGTACTTGGAGGACTGCC+TGG | 0.491036 | 8.1:-37504021 | MS.gene60983:CDS |
| GTTGAAGGAGGTCTGGAAGA+TGG | 0.497831 | 8.1:-37503895 | MS.gene60983:CDS |
| CCTTCAACTGGTCCAGTACC+AGG | 0.498932 | 8.1:+37503910 | None:intergenic |
| GGGTGATGGAGGACTGCCTG+GGG | 0.503356 | 8.1:-37504112 | MS.gene60983:CDS |
| GTTCCACAAACCACTGGATC+CGG | 0.504315 | 8.1:-37504180 | MS.gene60983:CDS |
| GTTCCTGGTGAAGGAGGAGA+TGG | 0.507827 | 8.1:-37503940 | MS.gene60983:CDS |
| GCACTGCCTGGAGGACTTCG+TGG | 0.511832 | 8.1:-37504075 | MS.gene60983:CDS |
| GAAGAAGGTTTGGGTGATGG+AGG | 0.528136 | 8.1:-37504123 | MS.gene60983:CDS |
| CAGTCCTCCAAGAAGTCCTC+CGG | 0.535457 | 8.1:+37504161 | None:intergenic |
| CCTTCTTCACCTGGTACACC+AGG | 0.538917 | 8.1:+37504138 | None:intergenic |
| CCTCCATCTCCTCCTTCACC+AGG | 0.541018 | 8.1:+37503937 | None:intergenic |
| CCTGGTGTACCAGGTGAAGA+AGG | 0.547230 | 8.1:-37504138 | MS.gene60983:CDS |
| GGACTGCCTGGTGAATATCC+TGG | 0.547709 | 8.1:-37504009 | MS.gene60983:CDS |
| GGTACACCACGAAGTCCTCC+AGG | 0.556740 | 8.1:+37504069 | None:intergenic |
| GTACCAGGTGAAGAAGGTTT+GGG | 0.563176 | 8.1:-37504132 | MS.gene60983:CDS |
| GGATCCGGAGGACTTCTTGG+AGG | 0.568359 | 8.1:-37504165 | MS.gene60983:CDS |
| CTTCCAGTTCCACAAACCAC+TGG | 0.572690 | 8.1:-37504186 | MS.gene60983:CDS |
| CTTCGTGGTGTACCAGATGA+AGG | 0.572822 | 8.1:-37504060 | MS.gene60983:CDS |
| CCACCGGAATTCACACCACC+AGG | 0.574503 | 8.1:+37503991 | None:intergenic |
| GGGTTGATAGCCGTCTCCAC+CGG | 0.580996 | 8.1:+37503975 | None:intergenic |
| CCTGGTGAAGGAGGAGATGG+AGG | 0.591303 | 8.1:-37503937 | MS.gene60983:CDS |
| GGTGTGAATTCCGGTGGAGA+CGG | 0.598585 | 8.1:-37503985 | MS.gene60983:CDS |
| GCAGAAGTTCCTGGTGAAGG+AGG | 0.602305 | 8.1:-37503946 | MS.gene60983:CDS |
| GGTGCAGAAGTTCCTGGTGA+AGG | 0.603113 | 8.1:-37503949 | MS.gene60983:CDS |
| CTACGACAATCGTGATTCTG+AGG | 0.610908 | 8.1:-37504250 | MS.gene60983:CDS |
| TCACCCAAACCTTCTTCACC+TGG | 0.611572 | 8.1:+37504129 | None:intergenic |
| AAGAAGTCCTCCGGATCCAG+TGG | 0.628933 | 8.1:+37504170 | None:intergenic |
| CAGTGGTTTGTGGAACTGGA+AGG | 0.642892 | 8.1:+37504187 | None:intergenic |
| ACACCACCAGGATATTCACC+AGG | 0.650451 | 8.1:+37504003 | None:intergenic |
| GGATCTGGAGCACTGCCTGG+AGG | 0.653536 | 8.1:-37504084 | MS.gene60983:CDS |
| CCTGGTGGTGTGAATTCCGG+TGG | 0.656979 | 8.1:-37503991 | MS.gene60983:CDS |
| GGTGATGGAGGACTGCCTGG+GGG | 0.657183 | 8.1:-37504111 | MS.gene60983:CDS |
| GGTACTGGACCAGTTGAAGG+AGG | 0.657439 | 8.1:-37503907 | MS.gene60983:CDS |
| GGAGACGGCTATCAACCCGT+TGG | 0.670699 | 8.1:-37503970 | MS.gene60983:CDS |
| CCACAAACCACTGGATCCGG+AGG | 0.679712 | 8.1:-37504177 | MS.gene60983:CDS |
| CTGCCTGGTGAATATCCTGG+TGG | 0.687649 | 8.1:-37504006 | MS.gene60983:CDS |
| TTTGACGGCTTTCAACCACA+AGG | 0.714605 | 8.1:-37504216 | MS.gene60983:CDS |
| ACTGGAAGGTGATAACCTTG+TGG | 0.757808 | 8.1:+37504201 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATACATAGCCTTTTTCATC+AGG | + | chr8.1:37504329-37504348 | None:intergenic | 30.0% |
| !! | AATCGTGATTCTGAGGATTT+TGG | - | chr8.1:37503948-37503967 | MS.gene60983:CDS | 35.0% |
| !! | TTGGGTGATGAAAAAGTACT+TGG | - | chr8.1:37504158-37504177 | MS.gene60983:CDS | 35.0% |
| GTACCAGATGAAGGAGATTT+GGG | - | chr8.1:37504140-37504159 | MS.gene60983:CDS | 40.0% | |
| TGTACCAGATGAAGGAGATT+TGG | - | chr8.1:37504139-37504158 | MS.gene60983:CDS | 40.0% | |
| ! | ATTGCTCTCTTTGTGCTGTT+GGG | - | chr8.1:37503888-37503907 | MS.gene60983:CDS | 40.0% |
| ! | GAGGATTTTGGACGATTTGA+CGG | - | chr8.1:37503960-37503979 | MS.gene60983:CDS | 40.0% |
| !! | GGTGATGAAAAAGTACTTGG+AGG | - | chr8.1:37504161-37504180 | MS.gene60983:CDS | 40.0% |
| ACTGGAAGGTGATAACCTTG+TGG | + | chr8.1:37503993-37504012 | None:intergenic | 45.0% | |
| CATTGCTCTCTTTGTGCTGT+TGG | - | chr8.1:37503887-37503906 | MS.gene60983:CDS | 45.0% | |
| CTACGACAATCGTGATTCTG+AGG | - | chr8.1:37503941-37503960 | MS.gene60983:CDS | 45.0% | |
| GTACCAGGTGAAGAAGGTTT+GGG | - | chr8.1:37504059-37504078 | MS.gene60983:CDS | 45.0% | |
| TCACCCAAATCTCCTTCATC+TGG | + | chr8.1:37504146-37504165 | None:intergenic | 45.0% | |
| TGTACCAGGTGAAGAAGGTT+TGG | - | chr8.1:37504058-37504077 | MS.gene60983:CDS | 45.0% | |
| TTTGACGGCTTTCAACCACA+AGG | - | chr8.1:37503975-37503994 | MS.gene60983:CDS | 45.0% | |
| ! | TATCCTGGTGGTGTGAATTC+CGG | - | chr8.1:37504197-37504216 | MS.gene60983:CDS | 45.0% |
| !! | CATAGCCTTTTTCATCAGGC+AGG | + | chr8.1:37504325-37504344 | None:intergenic | 45.0% |
| ACACCACCAGGATATTCACC+AGG | + | chr8.1:37504191-37504210 | None:intergenic | 50.0% | |
| ACCAGGAACTTCTGCACCAA+CGG | + | chr8.1:37504240-37504259 | None:intergenic | 50.0% | |
| CTTCCAGTTCCACAAACCAC+TGG | - | chr8.1:37504005-37504024 | MS.gene60983:CDS | 50.0% | |
| CTTCGTGGTGTACCAGATGA+AGG | - | chr8.1:37504131-37504150 | MS.gene60983:CDS | 50.0% | |
| GGAGTCCTGCCTGATGAAAA+AGG | - | chr8.1:37504317-37504336 | MS.gene60983:CDS | 50.0% | |
| GGTGAAGAAGGTTTGGGTGA+TGG | - | chr8.1:37504065-37504084 | MS.gene60983:CDS | 50.0% | |
| GTTCCACAAACCACTGGATC+CGG | - | chr8.1:37504011-37504030 | MS.gene60983:CDS | 50.0% | |
| GTTGAAGGAGGTCTGGAAGA+TGG | - | chr8.1:37504296-37504315 | MS.gene60983:CDS | 50.0% | |
| TCACCCAAACCTTCTTCACC+TGG | + | chr8.1:37504065-37504084 | None:intergenic | 50.0% | |
| TCTTCCAGACCTCCTTCAAC+TGG | + | chr8.1:37504296-37504315 | None:intergenic | 50.0% | |
| ! | AAAGTACTTGGAGGACTGCC+TGG | - | chr8.1:37504170-37504189 | MS.gene60983:CDS | 50.0% |
| ! | CAGTGGTTTGTGGAACTGGA+AGG | + | chr8.1:37504007-37504026 | None:intergenic | 50.0% |
| ! | GAAGAAGGTTTGGGTGATGG+AGG | - | chr8.1:37504068-37504087 | MS.gene60983:CDS | 50.0% |
| ! | GATCCAGTGGTTTGTGGAAC+TGG | + | chr8.1:37504011-37504030 | None:intergenic | 50.0% |
| AAGAAGTCCTCCGGATCCAG+TGG | + | chr8.1:37504024-37504043 | None:intergenic | 55.0% | |
| ACTGGATCCGGAGGACTTCT+TGG | - | chr8.1:37504023-37504042 | MS.gene60983:CDS | 55.0% | |
| CAGTCCTCCAAGAAGTCCTC+CGG | + | chr8.1:37504033-37504052 | None:intergenic | 55.0% | |
| CCAGGAACTTCTGCACCAAC+GGG | + | chr8.1:37504239-37504258 | None:intergenic | 55.0% | |
| CCTGGTGTACCAGGTGAAGA+AGG | - | chr8.1:37504053-37504072 | MS.gene60983:CDS | 55.0% | |
| CCTTCAACTGGTCCAGTACC+AGG | + | chr8.1:37504284-37504303 | None:intergenic | 55.0% | |
| CCTTCTTCACCTGGTACACC+AGG | + | chr8.1:37504056-37504075 | None:intergenic | 55.0% | |
| CTGCCTGGTGAATATCCTGG+TGG | - | chr8.1:37504185-37504204 | MS.gene60983:CDS | 55.0% | |
| GCAGAAGTTCCTGGTGAAGG+AGG | - | chr8.1:37504245-37504264 | MS.gene60983:CDS | 55.0% | |
| GGACTGCCTGGTGAATATCC+TGG | - | chr8.1:37504182-37504201 | MS.gene60983:CDS | 55.0% | |
| GGTGCAGAAGTTCCTGGTGA+AGG | - | chr8.1:37504242-37504261 | MS.gene60983:CDS | 55.0% | |
| GGTGTGAATTCCGGTGGAGA+CGG | - | chr8.1:37504206-37504225 | MS.gene60983:CDS | 55.0% | |
| GTTCCTGGTGAAGGAGGAGA+TGG | - | chr8.1:37504251-37504270 | MS.gene60983:CDS | 55.0% | |
| TGGACCAGTTGAAGGAGGTC+TGG | - | chr8.1:37504289-37504308 | MS.gene60983:CDS | 55.0% | |
| ! | CCTGGTACTGGACCAGTTGA+AGG | - | chr8.1:37504281-37504300 | MS.gene60983:CDS | 55.0% |
| ! | GGTACTGGACCAGTTGAAGG+AGG | - | chr8.1:37504284-37504303 | MS.gene60983:CDS | 55.0% |
| !! | ATAGGATCTGGAGCACTGCC+TGG | - | chr8.1:37504104-37504123 | MS.gene60983:CDS | 55.0% |
| CCACAAACCACTGGATCCGG+AGG | - | chr8.1:37504014-37504033 | MS.gene60983:CDS | 60.0% | |
| CCACCGGAATTCACACCACC+AGG | + | chr8.1:37504203-37504222 | None:intergenic | 60.0% | |
| CCCGTTGGTGCAGAAGTTCC+TGG | - | chr8.1:37504236-37504255 | MS.gene60983:CDS | 60.0% | |
| CCTCCATCTCCTCCTTCACC+AGG | + | chr8.1:37504257-37504276 | None:intergenic | 60.0% | |
| CCTCCGGATCCAGTGGTTTG+TGG | + | chr8.1:37504017-37504036 | None:intergenic | 60.0% | |
| CCTGGTGAAGGAGGAGATGG+AGG | - | chr8.1:37504254-37504273 | MS.gene60983:CDS | 60.0% | |
| GATGGAGGACTGCCTGGTAC+TGG | - | chr8.1:37504269-37504288 | MS.gene60983:CDS | 60.0% | |
| GCTCCAGATCCTATACCCCC+AGG | + | chr8.1:37504098-37504117 | None:intergenic | 60.0% | |
| GGACTTCTTGGAGGACTGCC+TGG | - | chr8.1:37504035-37504054 | MS.gene60983:CDS | 60.0% | |
| GGAGACGGCTATCAACCCGT+TGG | - | chr8.1:37504221-37504240 | MS.gene60983:CDS | 60.0% | |
| GGATCCGGAGGACTTCTTGG+AGG | - | chr8.1:37504026-37504045 | MS.gene60983:CDS | 60.0% | |
| GGTACACCACGAAGTCCTCC+AGG | + | chr8.1:37504125-37504144 | None:intergenic | 60.0% | |
| ! | CCTGGTGGTGTGAATTCCGG+TGG | - | chr8.1:37504200-37504219 | MS.gene60983:CDS | 60.0% |
| ! | CTGCCTGGGGGTATAGGATC+TGG | - | chr8.1:37504092-37504111 | MS.gene60983:CDS | 60.0% |
| !! | GGGTTGATAGCCGTCTCCAC+CGG | + | chr8.1:37504219-37504238 | None:intergenic | 60.0% |
| !! | TGGGTGATGGAGGACTGCCT+GGG | - | chr8.1:37504078-37504097 | MS.gene60983:CDS | 60.0% |
| !! | TTGGGTGATGGAGGACTGCC+TGG | - | chr8.1:37504077-37504096 | MS.gene60983:CDS | 60.0% |
| GCACTGCCTGGAGGACTTCG+TGG | - | chr8.1:37504116-37504135 | MS.gene60983:CDS | 65.0% | |
| GGAGGACTGCCTGGTGTACC+AGG | - | chr8.1:37504044-37504063 | MS.gene60983:CDS | 65.0% | |
| GGAGGAGATGGAGGACTGCC+TGG | - | chr8.1:37504263-37504282 | MS.gene60983:CDS | 65.0% | |
| ! | GGAGGACTGCCTGGGGGTAT+AGG | - | chr8.1:37504086-37504105 | MS.gene60983:CDS | 65.0% |
| !! | GGATCTGGAGCACTGCCTGG+AGG | - | chr8.1:37504107-37504126 | MS.gene60983:CDS | 65.0% |
| !! | GGGTGATGGAGGACTGCCTG+GGG | - | chr8.1:37504079-37504098 | MS.gene60983:CDS | 65.0% |
| !! | GGTGATGGAGGACTGCCTGG+GGG | - | chr8.1:37504080-37504099 | MS.gene60983:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 37503864 | 37504349 | 37503864 | ID=MS.gene60983 |
| chr8.1 | mRNA | 37503864 | 37504349 | 37503864 | ID=MS.gene60983.t1;Parent=MS.gene60983 |
| chr8.1 | exon | 37503864 | 37504349 | 37503864 | ID=MS.gene60983.t1.exon1;Parent=MS.gene60983.t1 |
| chr8.1 | CDS | 37503864 | 37504349 | 37503864 | ID=cds.MS.gene60983.t1;Parent=MS.gene60983.t1 |
| Gene Sequence |
| Protein sequence |
