AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60993

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60993.t1 MTR_8g066780 95.506 356 15 1 1 355 1 356 0.0 689
MS.gene60993.t1 MTR_5g030010 55.000 380 138 10 1 355 1 372 3.82e-124 362
MS.gene60993.t1 MTR_5g064890 32.609 276 148 12 4 255 10 271 5.82e-31 120
MS.gene60993.t1 MTR_1g043410 35.465 172 100 4 42 208 20 185 9.01e-29 114
MS.gene60993.t1 MTR_1g070140 32.714 269 138 11 35 271 25 282 9.97e-29 114
MS.gene60993.t1 MTR_4g093830 36.508 189 97 6 14 187 17 197 1.20e-25 106
MS.gene60993.t1 MTR_2g009310 32.180 289 160 10 88 350 103 381 5.16e-25 104
MS.gene60993.t1 MTR_8g089880 55.128 78 33 1 110 187 123 198 3.26e-23 97.8
MS.gene60993.t1 MTR_4g106680 29.524 210 111 5 37 223 6 201 1.74e-22 96.7
MS.gene60993.t1 MTR_1g074190 30.738 244 135 9 70 281 53 294 2.19e-22 97.1
MS.gene60993.t1 MTR_5g034790 33.898 177 99 4 25 189 19 189 5.35e-22 95.9
MS.gene60993.t1 MTR_3g094000 37.671 146 80 4 43 188 31 165 1.26e-21 90.9
MS.gene60993.t1 MTR_6g007737 36.170 141 81 2 56 189 68 206 1.62e-21 94.7
MS.gene60993.t1 MTR_6g087150 36.429 140 80 4 50 187 32 164 2.08e-21 94.4
MS.gene60993.t1 MTR_7g096690 34.586 133 84 2 58 187 13 145 7.07e-21 92.4
MS.gene60993.t1 MTR_3g091620 39.007 141 74 2 55 185 32 170 6.55e-20 90.1
MS.gene60993.t1 MTR_8g063660 36.429 140 77 3 58 188 4 140 9.21e-20 89.4
MS.gene60993.t1 MTR_5g064990 33.974 156 86 5 37 187 45 188 1.12e-19 89.7
MS.gene60993.t1 MTR_7g073200 32.386 176 99 5 41 206 13 178 1.35e-19 85.5
MS.gene60993.t1 MTR_5g064980 33.974 156 97 5 34 187 31 182 1.46e-19 89.4
MS.gene60993.t1 MTR_1g082550 29.500 200 117 7 56 246 46 230 2.53e-19 88.6
MS.gene60993.t1 MTR_3g078470 33.103 145 92 4 42 185 7 147 3.82e-19 85.5
MS.gene60993.t1 MTR_3g099650 32.353 136 82 3 55 188 55 182 5.22e-19 87.4
MS.gene60993.t1 MTR_7g113800 26.738 187 119 5 55 231 40 218 5.77e-19 87.4
MS.gene60993.t1 MTR_2g096350 38.281 128 68 4 96 214 90 215 8.73e-19 84.3
MS.gene60993.t1 MTR_7g079280 44.828 87 45 1 100 186 69 152 1.12e-18 83.2
MS.gene60993.t1 MTR_7g116220 50.685 73 35 1 114 185 70 142 1.13e-18 83.2
MS.gene60993.t1 MTR_5g026730 30.000 140 94 1 48 187 4 139 1.33e-18 82.8
MS.gene60993.t1 MTR_4g022410 34.395 157 81 6 34 187 21 158 1.86e-18 82.8
MS.gene60993.t1 MTR_1g492830 32.867 143 88 4 58 198 8 144 2.63e-18 83.2
MS.gene60993.t1 MTR_3g110190 32.941 170 94 7 58 217 14 173 3.34e-18 84.7
MS.gene60993.t1 MTR_4g129420 32.184 174 107 6 70 240 73 238 6.49e-18 85.1
MS.gene60993.t1 MTR_4g120270 48.649 74 34 1 114 187 80 149 1.18e-17 82.4
MS.gene60993.t1 MTR_5g026720 31.613 155 98 3 48 199 4 153 1.19e-17 79.7
MS.gene60993.t1 MTR_3g078450 35.652 115 72 1 71 185 35 147 3.46e-17 79.7
MS.gene60993.t1 MTR_7g116200 37.500 136 67 3 61 185 8 136 3.56e-17 78.6
MS.gene60993.t1 MTR_4g063070 28.289 152 91 2 55 194 243 388 4.22e-17 82.0
MS.gene60993.t1 MTR_3g100400 31.765 170 96 5 34 187 36 201 4.77e-17 82.0
MS.gene60993.t1 MTR_5g066250 35.766 137 81 2 51 187 76 205 5.04e-17 82.0
MS.gene60993.t1 MTR_1g070425 46.053 76 40 1 114 188 68 143 6.98e-17 78.2
MS.gene60993.t1 MTR_3g086700 31.414 191 104 7 59 242 1 171 8.03e-17 78.2
MS.gene60993.t1 MTR_7g116210 48.611 72 34 1 114 185 10 78 8.67e-17 75.9
MS.gene60993.t1 MTR_8g071815 33.333 138 84 2 48 185 4 133 8.78e-17 77.8
MS.gene60993.t1 MTR_2g090905 35.652 115 69 2 78 187 2 116 9.61e-17 76.3
MS.gene60993.t1 MTR_7g116180 46.053 76 40 1 114 188 67 142 9.97e-17 77.4
MS.gene60993.t1 MTR_2g044570 61.818 55 20 1 136 189 76 130 1.55e-16 79.3
MS.gene60993.t1 MTR_8g009190 36.025 161 83 8 35 188 4 151 4.58e-16 78.2
MS.gene60993.t1 MTR_5g082940 41.892 74 43 0 114 187 75 148 6.46e-16 75.1
MS.gene60993.t1 MTR_8g092840 37.719 114 62 3 109 214 108 220 6.48e-16 76.6
MS.gene60993.t1 MTR_1g100737 40.260 77 43 1 114 187 92 168 6.76e-16 75.5
MS.gene60993.t1 MTR_2g090950 33.628 113 70 2 80 187 56 168 8.07e-16 76.3
MS.gene60993.t1 MTR_8g073120 31.655 139 84 2 47 185 14 141 9.24e-16 75.5
MS.gene60993.t1 MTR_5g024730 30.000 150 85 3 35 184 18 147 9.38e-16 75.9
MS.gene60993.t1 MTR_8g019710 51.613 62 30 0 126 187 38 99 9.40e-16 73.6
MS.gene60993.t1 MTR_8g092960 37.719 114 62 3 109 214 98 210 1.28e-15 75.5
MS.gene60993.t1 MTR_3g086730 30.909 165 87 6 59 218 1 143 1.38e-15 75.5
MS.gene60993.t1 MTR_4g056590 45.070 71 34 1 114 184 100 165 3.36e-15 73.6
MS.gene60993.t1 MTR_8g028465 32.667 150 80 3 53 186 14 158 4.44e-15 73.6
MS.gene60993.t1 MTR_5g076990 29.747 158 91 5 36 187 8 151 4.47e-15 75.5
MS.gene60993.t1 MTR_3g071300 31.579 133 77 3 58 187 30 151 5.54e-15 72.4
MS.gene60993.t1 MTR_7g076250 33.028 109 70 3 114 222 63 168 5.55e-15 72.8
MS.gene60993.t1 MTR_7g108855 43.836 73 41 0 115 187 67 139 6.41e-15 72.8
MS.gene60993.t1 MTR_8g093010 35.556 135 73 4 55 188 59 180 8.21e-15 73.2
MS.gene60993.t1 MTR_2g436100 44.737 76 40 1 112 187 91 164 1.17e-14 73.9
MS.gene60993.t1 MTR_3g078120 29.600 125 86 2 65 187 6 130 1.24e-14 70.9
MS.gene60993.t1 MTR_8g074900 46.479 71 32 1 114 184 158 222 1.46e-14 72.4
MS.gene60993.t1 MTR_4g094725 29.730 148 90 3 39 184 13 148 1.62e-14 70.9
MS.gene60993.t1 MTR_8g092970 46.667 75 40 0 140 214 129 203 1.76e-14 72.0
MS.gene60993.t1 MTR_3g464780 46.914 81 41 2 112 190 100 180 2.53e-14 72.4
MS.gene60993.t1 MTR_5g009210 33.333 132 80 2 54 185 235 358 2.64e-14 73.6
MS.gene60993.t1 MTR_8g092985 38.298 94 57 1 114 207 111 203 3.02e-14 71.2
MS.gene60993.t1 MTR_8g092930 38.298 94 57 1 114 207 112 204 3.17e-14 71.6
MS.gene60993.t1 MTR_6g055030 30.168 179 106 5 47 222 39 201 3.18e-14 72.8
MS.gene60993.t1 MTR_8g023400 46.667 75 37 2 113 187 91 162 3.42e-14 72.8
MS.gene60993.t1 MTR_4g030070 36.792 106 61 3 100 202 69 171 3.65e-14 71.2
MS.gene60993.t1 MTR_5g022920 64.444 45 16 0 140 184 187 231 4.17e-14 71.2
MS.gene60993.t1 MTR_8g066740 28.481 158 93 5 36 187 8 151 5.08e-14 72.4
MS.gene60993.t1 MTR_5g009200 34.091 132 79 3 54 185 270 393 5.19e-14 72.8
MS.gene60993.t1 MTR_2g041200 32.374 139 81 3 57 190 27 157 5.60e-14 69.7
MS.gene60993.t1 MTR_2g055790 30.534 131 73 4 58 188 34 146 5.66e-14 70.1
MS.gene60993.t1 MTR_8g099515 40.741 81 48 0 113 193 308 388 6.24e-14 72.8
MS.gene60993.t1 MTR_7g117540 50.769 65 27 2 123 187 87 146 6.27e-14 71.6
MS.gene60993.t1 MTR_1g090480 31.343 134 86 3 56 186 16 146 7.89e-14 69.3
MS.gene60993.t1 MTR_8g092990 59.184 49 20 0 140 188 139 187 7.97e-14 70.5
MS.gene60993.t1 MTR_5g092790 56.522 46 20 0 142 187 185 230 1.12e-13 70.1
MS.gene60993.t1 MTR_8g099495 37.895 95 49 1 101 185 296 390 1.18e-13 72.0
MS.gene60993.t1 MTR_5g092750 56.522 46 20 0 142 187 185 230 1.31e-13 70.1
MS.gene60993.t1 MTR_2g035800 30.986 142 83 4 58 199 32 158 1.70e-13 68.2
MS.gene60993.t1 MTR_7g079290 40.541 74 42 1 114 185 125 198 2.03e-13 68.9
MS.gene60993.t1 MTR_1g056350 40.449 89 50 2 113 199 73 160 2.25e-13 67.8
MS.gene60993.t1 MTR_3g078520 35.484 93 51 2 117 209 78 161 3.02e-13 67.4
MS.gene60993.t1 MTR_5g009190 41.096 73 43 0 113 185 265 337 3.17e-13 70.1
MS.gene60993.t1 MTR_3g114500 54.000 50 23 0 138 187 67 116 3.74e-13 66.2
MS.gene60993.t1 MTR_8g032690 38.462 65 40 0 139 203 16 80 4.64e-13 65.5
MS.gene60993.t1 MTR_3g095560 62.222 45 17 0 140 184 134 178 4.82e-13 68.6
MS.gene60993.t1 MTR_3g116000 30.769 130 72 4 60 184 30 146 6.03e-13 67.4
MS.gene60993.t1 MTR_3g078540 30.147 136 73 5 55 186 6 123 3.92e-12 63.9
MS.gene60993.t1 MTR_6g055030 39.773 88 43 2 140 222 88 170 4.70e-12 66.2
MS.gene60993.t1 MTR_3g012550 27.068 133 96 1 54 186 21 152 6.35e-12 63.9
MS.gene60993.t1 MTR_6g007680 53.191 47 22 0 141 187 65 111 1.07e-11 62.8
MS.gene60993.t1 MTR_1g090460 46.053 76 31 3 127 195 83 155 1.20e-11 62.8
MS.gene60993.t1 MTR_7g117350 37.975 79 45 1 114 188 282 360 1.72e-11 65.1
MS.gene60993.t1 MTR_7g117350 37.975 79 45 1 114 188 290 368 1.79e-11 65.1
MS.gene60993.t1 MTR_3g081030 55.319 47 21 0 142 188 119 165 2.41e-11 63.2
MS.gene60993.t1 MTR_4g108830 50.000 48 24 0 140 187 144 191 4.18e-11 62.0
MS.gene60993.t1 MTR_3g087900 50.847 59 27 2 141 198 68 125 4.60e-11 60.8
MS.gene60993.t1 MTR_3g091200 30.769 130 80 2 63 186 250 375 4.82e-11 63.9
MS.gene60993.t1 MTR_5g071930 34.043 94 55 2 114 207 66 152 4.85e-11 62.0
MS.gene60993.t1 MTR_4g108830 50.000 48 24 0 140 187 172 219 5.13e-11 62.0
MS.gene60993.t1 MTR_5g096410 36.170 94 46 2 107 187 234 326 6.68e-11 63.2
MS.gene60993.t1 MTR_7g059360 53.191 47 22 0 141 187 157 203 6.92e-11 62.0
MS.gene60993.t1 MTR_5g096410 36.170 94 46 2 107 187 243 335 7.07e-11 63.2
MS.gene60993.t1 MTR_5g096410 36.170 94 46 2 107 187 246 338 7.11e-11 63.2
MS.gene60993.t1 MTR_1g026070 45.098 51 28 0 137 187 66 116 8.91e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60993.t1 AT4G17905 49.765 213 91 6 2 199 3 214 1.01e-61 200
MS.gene60993.t1 AT4G17905 49.765 213 91 6 2 199 29 240 3.02e-61 200
MS.gene60993.t1 AT5G17600 38.544 371 180 12 7 348 5 356 2.44e-59 196
MS.gene60993.t1 AT3G03550 38.484 343 154 12 1 314 1 315 4.66e-56 187
MS.gene60993.t1 AT1G04360 41.667 156 64 4 55 191 41 188 8.41e-30 118
MS.gene60993.t1 AT5G43420 39.744 156 65 5 55 187 34 183 9.68e-30 117
MS.gene60993.t1 AT1G72220 30.682 264 146 7 3 233 7 266 1.17e-28 115
MS.gene60993.t1 AT5G10380 37.572 173 96 3 109 281 104 264 7.02e-25 103
MS.gene60993.t1 AT4G40070 30.500 200 120 5 36 234 31 212 9.01e-24 100
MS.gene60993.t1 AT1G23980 36.306 157 81 5 56 200 50 199 3.55e-23 99.4
MS.gene60993.t1 AT5G40250 29.907 214 134 4 56 253 42 255 1.51e-22 97.8
MS.gene60993.t1 AT1G72200 29.851 201 130 5 55 249 59 254 4.17e-22 97.1
MS.gene60993.t1 AT2G20030 32.461 191 117 7 35 221 20 202 5.90e-22 96.3
MS.gene60993.t1 AT4G33565 44.231 104 54 2 109 210 186 287 8.94e-22 95.5
MS.gene60993.t1 AT1G22500 32.075 159 102 3 48 205 28 181 5.37e-21 93.6
MS.gene60993.t1 AT3G10910 38.129 139 76 4 54 188 28 160 1.99e-20 87.8
MS.gene60993.t1 AT3G18930 33.529 170 72 3 55 188 38 202 2.52e-20 91.7
MS.gene60993.t1 AT3G18930 33.529 170 72 3 55 188 38 202 2.52e-20 91.7
MS.gene60993.t1 AT3G18930 33.529 170 72 3 55 188 38 202 2.52e-20 91.7
MS.gene60993.t1 AT4G28890 36.478 159 86 6 37 187 17 168 3.01e-20 91.7
MS.gene60993.t1 AT3G48030 52.381 84 37 2 114 196 102 183 1.04e-19 88.2
MS.gene60993.t1 AT3G48030 52.381 84 37 2 114 196 180 261 1.29e-19 89.0
MS.gene60993.t1 AT5G01880 47.500 80 40 1 114 193 79 156 1.50e-19 84.7
MS.gene60993.t1 AT3G05200 28.333 180 111 5 47 221 33 199 2.76e-19 88.6
MS.gene60993.t1 AT4G15975 51.351 74 33 1 114 187 51 121 1.10e-18 84.3
MS.gene60993.t1 AT5G57750 35.507 138 78 5 56 186 33 166 1.77e-18 83.2
MS.gene60993.t1 AT2G18650 40.179 112 65 2 86 197 72 181 4.49e-18 85.5
MS.gene60993.t1 AT5G27420 29.775 178 103 6 47 207 28 200 1.11e-17 83.6
MS.gene60993.t1 AT1G76410 34.058 138 83 3 47 184 17 146 1.28e-17 80.1
MS.gene60993.t1 AT1G28040 36.301 146 78 4 47 186 161 297 1.54e-17 82.4
MS.gene60993.t1 AT2G47560 47.297 74 39 0 114 187 80 153 1.61e-17 80.9
MS.gene60993.t1 AT2G35000 34.328 134 76 4 62 187 51 180 3.11e-17 82.4
MS.gene60993.t1 AT4G09100 46.667 75 39 1 114 187 56 130 5.12e-17 77.0
MS.gene60993.t1 AT2G27940 45.205 73 37 1 114 186 115 184 7.11e-17 79.3
MS.gene60993.t1 AT3G62690 49.315 73 35 1 115 187 88 158 9.51e-17 79.3
MS.gene60993.t1 AT3G16720 48.148 81 39 2 110 190 90 167 2.83e-16 79.0
MS.gene60993.t1 AT1G53820 45.122 82 42 2 114 195 95 173 3.42e-16 78.6
MS.gene60993.t1 AT4G30400 41.053 95 55 1 90 184 83 176 3.76e-16 79.7
MS.gene60993.t1 AT1G35330 27.211 147 98 3 47 187 31 174 1.11e-15 77.4
MS.gene60993.t1 AT5G66070 37.607 117 59 3 68 184 140 242 1.52e-15 75.9
MS.gene60993.t1 AT5G05280 43.137 102 45 4 89 188 69 159 1.54e-15 74.3
MS.gene60993.t1 AT5G66070 63.043 46 17 0 139 184 173 218 2.66e-15 74.7
MS.gene60993.t1 AT5G66070 63.043 46 17 0 139 184 173 218 2.66e-15 74.7
MS.gene60993.t1 AT3G61550 41.111 90 44 2 106 186 91 180 2.95e-15 74.3
MS.gene60993.t1 AT2G46160 43.678 87 43 1 114 194 104 190 3.09e-15 74.3
MS.gene60993.t1 AT2G46160 43.678 87 43 1 114 194 104 190 3.09e-15 74.3
MS.gene60993.t1 AT4G09130 42.667 75 42 1 114 187 91 165 3.77e-15 76.3
MS.gene60993.t1 AT2G35910 39.535 86 50 1 114 199 121 204 5.15e-15 73.6
MS.gene60993.t1 AT2G46494 34.074 135 84 3 54 186 229 360 5.26e-15 75.9
MS.gene60993.t1 AT4G10150 27.451 204 127 5 40 232 10 203 5.68e-15 73.9
MS.gene60993.t1 AT2G46493 36.000 100 62 1 87 186 85 182 5.92e-15 72.8
MS.gene60993.t1 AT2G17450 27.815 151 96 3 34 184 7 144 6.28e-15 72.8
MS.gene60993.t1 AT2G42360 29.577 142 93 3 46 187 19 153 7.38e-15 73.6
MS.gene60993.t1 AT3G14320 35.577 104 56 2 114 217 63 155 8.76e-15 72.8
MS.gene60993.t1 AT5G05810 44.595 74 39 1 114 187 120 191 9.62e-15 75.5
MS.gene60993.t1 AT5G07040 30.952 126 81 2 63 186 17 138 1.04e-14 71.6
MS.gene60993.t1 AT4G10160 29.333 150 95 4 90 232 44 189 1.18e-14 72.8
MS.gene60993.t1 AT4G09120 41.333 75 43 1 114 187 94 168 1.25e-14 74.3
MS.gene60993.t1 AT3G11110 33.333 144 84 5 45 187 20 152 1.86e-14 70.9
MS.gene60993.t1 AT3G60220 46.479 71 37 1 120 189 94 164 1.91e-14 73.9
MS.gene60993.t1 AT4G09110 40.000 75 44 1 114 187 94 168 2.19e-14 73.2
MS.gene60993.t1 AT2G34990 29.231 130 89 2 56 184 11 138 2.47e-14 73.2
MS.gene60993.t1 AT2G34990 44.444 72 39 1 114 184 89 160 2.62e-14 73.2
MS.gene60993.t1 AT2G42350 36.937 111 56 3 76 185 46 143 2.80e-14 71.6
MS.gene60993.t1 AT5G06490 28.859 149 79 4 63 186 27 173 4.05e-14 70.9
MS.gene60993.t1 AT1G32361 38.776 98 50 3 88 184 108 196 4.25e-14 72.8
MS.gene60993.t1 AT1G49220 30.822 146 90 4 40 185 44 178 4.91e-14 71.6
MS.gene60993.t1 AT2G17730 60.870 46 18 0 142 187 196 241 5.33e-14 71.2
MS.gene60993.t1 AT1G49230 33.333 147 85 5 39 184 39 173 7.63e-14 70.5
MS.gene60993.t1 AT1G20823 29.412 136 94 2 49 184 20 153 8.75e-14 69.7
MS.gene60993.t1 AT4G35480 44.776 67 30 1 140 199 111 177 1.12e-13 69.7
MS.gene60993.t1 AT4G35840 65.116 43 15 0 142 184 191 233 1.39e-13 70.1
MS.gene60993.t1 AT2G35420 43.662 71 38 1 114 184 77 145 1.56e-13 70.1
MS.gene60993.t1 AT5G47610 43.750 64 36 0 131 194 96 159 1.99e-13 68.2
MS.gene60993.t1 AT5G53110 34.127 126 79 2 61 186 253 374 3.07e-13 70.5
MS.gene60993.t1 AT5G46650 31.818 132 83 3 58 187 34 160 3.15e-13 69.7
MS.gene60993.t1 AT1G72310 51.613 62 28 2 141 200 126 187 4.66e-13 69.7
MS.gene60993.t1 AT2G25410 45.679 81 39 3 109 186 293 371 7.87e-13 69.3
MS.gene60993.t1 AT3G60966 43.836 73 39 1 142 212 62 134 1.30e-12 65.1
MS.gene60993.t1 AT1G49210 27.333 150 98 4 35 184 39 177 1.91e-12 66.6
MS.gene60993.t1 AT4G17920 30.719 153 93 4 40 187 11 155 1.91e-12 67.4
MS.gene60993.t1 AT2G18670 39.241 79 47 1 109 187 78 155 2.59e-12 65.1
MS.gene60993.t1 AT1G49200 25.503 149 100 4 36 184 41 178 4.84e-12 65.5
MS.gene60993.t1 AT1G33480 43.548 62 30 1 123 184 86 142 8.21e-12 65.1
MS.gene60993.t1 AT2G46495 43.373 83 36 2 110 186 287 364 9.79e-12 65.9
MS.gene60993.t1 AT2G46495 43.373 83 36 2 110 186 259 336 9.95e-12 65.9
MS.gene60993.t1 AT1G74410 48.980 49 25 0 139 187 173 221 1.23e-11 64.3
MS.gene60993.t1 AT1G80400 56.522 46 19 1 142 187 355 399 1.96e-11 65.1
MS.gene60993.t1 AT4G24015 56.522 46 20 0 142 187 105 150 2.21e-11 62.4
MS.gene60993.t1 AT4G17245 45.312 64 34 1 140 202 100 163 2.29e-11 62.0
MS.gene60993.t1 AT3G19140 39.744 78 44 2 110 187 50 124 2.37e-11 61.6
MS.gene60993.t1 AT1G51930 54.348 46 21 0 142 187 80 125 2.53e-11 61.2
MS.gene60993.t1 AT5G58580 34.783 92 59 1 114 205 111 201 3.07e-11 63.9
MS.gene60993.t1 AT4G30370 38.667 75 44 1 114 188 89 161 4.17e-11 61.6
MS.gene60993.t1 AT5G42200 47.917 48 25 0 140 187 102 149 5.57e-11 60.8
MS.gene60993.t1 AT3G19910 40.789 76 43 2 114 189 261 334 8.70e-11 62.8

Find 46 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCCATGTTGTTGGATAAT+TGG 0.141569 8.1:-37706040 None:intergenic
TCCTCTTGTTATTGCAATCT+TGG 0.197966 8.1:+37706197 MS.gene60993:CDS
AACTGTCAAGTTTCAACCTT+TGG 0.310964 8.1:+37706348 MS.gene60993:CDS
GATCGGAAGGTCAGTGTCTA+TGG 0.318014 8.1:+37706818 MS.gene60993:CDS
GTAAAATTGATAGAGGAAAA+AGG 0.320392 8.1:+37707054 MS.gene60993:CDS
AACGATCTCTTCATTGCAAT+TGG 0.329260 8.1:-37707008 None:intergenic
ATGGATCATTCATTTCAAAA+TGG 0.338983 8.1:+37706837 MS.gene60993:CDS
CCTCTTGTTATTGCAATCTT+GGG 0.352229 8.1:+37706198 MS.gene60993:CDS
ATCCATGTTGTTGGATAATT+GGG 0.383221 8.1:-37706039 None:intergenic
CAATCTTTGCTGTTTATGTA+AGG 0.387421 8.1:-37706063 None:intergenic
TCACTTGGTAAAATTGATAG+AGG 0.401304 8.1:+37707047 MS.gene60993:CDS
TATTGAAATGGTGGAGGTGG+TGG 0.407936 8.1:-37706144 None:intergenic
TGAAAAGTTTCATTTCTTGA+AGG 0.410985 8.1:-37706648 None:intergenic
ATAACAAGAGGAGAGAAATA+TGG 0.423360 8.1:-37706186 None:intergenic
ATCTTCCTTGTATTGATACA+TGG 0.431852 8.1:+37706526 MS.gene60993:CDS
CATAAATAAAATTCTCAAAC+AGG 0.433181 8.1:-37707089 None:intergenic
AACACATCATGTTCAAGTTC+CGG 0.446335 8.1:+37706608 MS.gene60993:CDS
TCACTAAAAGCGCGCAATGA+AGG 0.452791 8.1:-37706735 None:intergenic
TGAAATGGTGGAGGTGGTGG+AGG 0.461217 8.1:-37706141 None:intergenic
ATGTAAGGAATCCATGTTGT+TGG 0.471174 8.1:-37706048 None:intergenic
TCATCATCAGGGTATTGAAA+TGG 0.478045 8.1:-37706156 None:intergenic
AACAGCAAAGATTGTTCTCA+AGG 0.495067 8.1:+37706072 MS.gene60993:CDS
GGGTATTGAAATGGTGGAGG+TGG 0.504961 8.1:-37706147 None:intergenic
TTGTGAAGAAGGATGTTCAA+TGG 0.517950 8.1:+37706905 MS.gene60993:CDS
TCATGTTCAAGTTCCGGCTA+CGG 0.523483 8.1:+37706614 MS.gene60993:CDS
GATTTCATCAACTGGGTCAG+TGG 0.533453 8.1:-37706307 None:intergenic
AAGGAGAAAGTAGTAAATCA+AGG 0.536690 8.1:+37706952 MS.gene60993:CDS
GGAAGTTCTATAACCGTAGC+CGG 0.557787 8.1:-37706627 None:intergenic
TTGAGCATCATCTAAACCAA+AGG 0.567832 8.1:-37706364 None:intergenic
CCCAAGATTGCAATAACAAG+AGG 0.569705 8.1:-37706198 None:intergenic
AGTGATTTATGTAATTTGCA+AGG 0.571415 8.1:+37706753 MS.gene60993:CDS
TTGAGCCATGTATCAATACA+AGG 0.575815 8.1:-37706531 None:intergenic
TTTGTTGGATTTCATCAACT+GGG 0.585830 8.1:-37706314 None:intergenic
TCATCAGGGTATTGAAATGG+TGG 0.592002 8.1:-37706153 None:intergenic
GATGAAGTTTGTGAATCGAT+CGG 0.596066 8.1:+37706801 MS.gene60993:CDS
GAAGGATGTTCAATGGATGT+TGG 0.605378 8.1:+37706912 MS.gene60993:CDS
TCAGGGTATTGAAATGGTGG+AGG 0.616494 8.1:-37706150 None:intergenic
ACACTAATATCTAAATATTG+TGG 0.619136 8.1:+37706258 MS.gene60993:CDS
AAGTTTGTGAATCGATCGGA+AGG 0.619893 8.1:+37706805 MS.gene60993:CDS
AGAAGAGCAACAAATAGTTG+TGG 0.625083 8.1:+37706671 MS.gene60993:CDS
TCATAGTAGAGTTGAGTCGA+AGG 0.628315 8.1:+37706710 MS.gene60993:CDS
TAAACTATATAGCACCATTG+TGG 0.641826 8.1:-37706114 None:intergenic
AATACGCTAGAACGACATAG+AGG 0.649889 8.1:-37706570 None:intergenic
GTCCCAATTATCCAACAACA+TGG 0.686792 8.1:+37706037 MS.gene60993:CDS
GGTTCATCAAAGATATCACA+AGG 0.689439 8.1:+37706933 MS.gene60993:CDS
GTAGCGTGTATTGTCCACAA+TGG 0.715092 8.1:+37706100 MS.gene60993:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAAAATGGTTTTTTAAT+TGG + chr8.1:37706849-37706868 MS.gene60993:CDS 15.0%
!! ACACTAATATCTAAATATTG+TGG + chr8.1:37706258-37706277 MS.gene60993:CDS 20.0%
!! TTTGTATTTAAAAACTGCAA+TGG - chr8.1:37706397-37706416 None:intergenic 20.0%
!!! GCAGTTTTTAAATACAAAAA+AGG + chr8.1:37706399-37706418 MS.gene60993:CDS 20.0%
! ATGGATCATTCATTTCAAAA+TGG + chr8.1:37706837-37706856 MS.gene60993:CDS 25.0%
! GTAAAATTGATAGAGGAAAA+AGG + chr8.1:37707054-37707073 MS.gene60993:CDS 25.0%
! TGAAAAGTTTCATTTCTTGA+AGG - chr8.1:37706651-37706670 None:intergenic 25.0%
!! AGTGATTTATGTAATTTGCA+AGG + chr8.1:37706753-37706772 MS.gene60993:CDS 25.0%
!!! TTTTTTAATTGGTGATGTGT+TGG + chr8.1:37706860-37706879 MS.gene60993:CDS 25.0%
AAGGAGAAAGTAGTAAATCA+AGG + chr8.1:37706952-37706971 MS.gene60993:CDS 30.0%
AATCCATGTTGTTGGATAAT+TGG - chr8.1:37706043-37706062 None:intergenic 30.0%
AGTTAGTAGTAAAAAAGCAG+TGG - chr8.1:37706232-37706251 None:intergenic 30.0%
ATAACAAGAGGAGAGAAATA+TGG - chr8.1:37706189-37706208 None:intergenic 30.0%
ATCCATGTTGTTGGATAATT+GGG - chr8.1:37706042-37706061 None:intergenic 30.0%
TAAACTATATAGCACCATTG+TGG - chr8.1:37706117-37706136 None:intergenic 30.0%
TACAAGGAAGATGAAAAACA+TGG - chr8.1:37706518-37706537 None:intergenic 30.0%
TCACTTGGTAAAATTGATAG+AGG + chr8.1:37707047-37707066 MS.gene60993:CDS 30.0%
TGAAAAACATGGTTACACAT+TGG - chr8.1:37706507-37706526 None:intergenic 30.0%
TTTGTTGGATTTCATCAACT+GGG - chr8.1:37706317-37706336 None:intergenic 30.0%
! ATCTTCCTTGTATTGATACA+TGG + chr8.1:37706526-37706545 MS.gene60993:CDS 30.0%
! ATGAAGAGATCGTTTTCTAA+TGG + chr8.1:37707017-37707036 MS.gene60993:CDS 30.0%
! GATGAAGATTTTTGTGAAGA+AGG + chr8.1:37706894-37706913 MS.gene60993:CDS 30.0%
! TCTAATGGAAGATTTTCACT+TGG + chr8.1:37707032-37707051 MS.gene60993:CDS 30.0%
! TTTTGTTGGATTTCATCAAC+TGG - chr8.1:37706318-37706337 None:intergenic 30.0%
!! CAATCTTTGCTGTTTATGTA+AGG - chr8.1:37706066-37706085 None:intergenic 30.0%
!!! ATGGTTTTGAATCATCATCA+GGG - chr8.1:37706170-37706189 None:intergenic 30.0%
!!! TATGGTTTTGAATCATCATC+AGG - chr8.1:37706171-37706190 None:intergenic 30.0%
AACAGCAAAGATTGTTCTCA+AGG + chr8.1:37706072-37706091 MS.gene60993:CDS 35.0%
AACGATCTCTTCATTGCAAT+TGG - chr8.1:37707011-37707030 None:intergenic 35.0%
AACTGTCAAGTTTCAACCTT+TGG + chr8.1:37706348-37706367 MS.gene60993:CDS 35.0%
AGAAGAGCAACAAATAGTTG+TGG + chr8.1:37706671-37706690 MS.gene60993:CDS 35.0%
ATGTAAGGAATCCATGTTGT+TGG - chr8.1:37706051-37706070 None:intergenic 35.0%
ATTGATAGAGGAAAAAGGCA+AGG + chr8.1:37707059-37707078 MS.gene60993:CDS 35.0%
GATGAAGTTTGTGAATCGAT+CGG + chr8.1:37706801-37706820 MS.gene60993:CDS 35.0%
GGTTCATCAAAGATATCACA+AGG + chr8.1:37706933-37706952 MS.gene60993:CDS 35.0%
TTGAGCATCATCTAAACCAA+AGG - chr8.1:37706367-37706386 None:intergenic 35.0%
TTGAGCCATGTATCAATACA+AGG - chr8.1:37706534-37706553 None:intergenic 35.0%
TTGTGAAGAAGGATGTTCAA+TGG + chr8.1:37706905-37706924 MS.gene60993:CDS 35.0%
! AACACATCATGTTCAAGTTC+CGG + chr8.1:37706608-37706627 MS.gene60993:CDS 35.0%
! TTGAAACTTGACAGTTTTCG+CGG - chr8.1:37706344-37706363 None:intergenic 35.0%
!! CCTCTTGTTATTGCAATCTT+GGG + chr8.1:37706198-37706217 MS.gene60993:CDS 35.0%
!! TCATCATCAGGGTATTGAAA+TGG - chr8.1:37706159-37706178 None:intergenic 35.0%
!! TCCTCTTGTTATTGCAATCT+TGG + chr8.1:37706197-37706216 MS.gene60993:CDS 35.0%
AAGTTTGTGAATCGATCGGA+AGG + chr8.1:37706805-37706824 MS.gene60993:CDS 40.0%
AATACGCTAGAACGACATAG+AGG - chr8.1:37706573-37706592 None:intergenic 40.0%
CCCAAGATTGCAATAACAAG+AGG - chr8.1:37706201-37706220 None:intergenic 40.0%
GAAGGATGTTCAATGGATGT+TGG + chr8.1:37706912-37706931 MS.gene60993:CDS 40.0%
GTCCCAATTATCCAACAACA+TGG + chr8.1:37706037-37706056 MS.gene60993:CDS 40.0%
TCATAGTAGAGTTGAGTCGA+AGG + chr8.1:37706710-37706729 MS.gene60993:CDS 40.0%
!! TCATCAGGGTATTGAAATGG+TGG - chr8.1:37706156-37706175 None:intergenic 40.0%
GATTTCATCAACTGGGTCAG+TGG - chr8.1:37706310-37706329 None:intergenic 45.0%
GGAAGTTCTATAACCGTAGC+CGG - chr8.1:37706630-37706649 None:intergenic 45.0%
GTAGCGTGTATTGTCCACAA+TGG + chr8.1:37706100-37706119 MS.gene60993:CDS 45.0%
! AGTTTTCGCGGCGATTTTGT+TGG - chr8.1:37706332-37706351 None:intergenic 45.0%
! TATTGAAATGGTGGAGGTGG+TGG - chr8.1:37706147-37706166 None:intergenic 45.0%
! TCACTAAAAGCGCGCAATGA+AGG - chr8.1:37706738-37706757 None:intergenic 45.0%
! TCATGTTCAAGTTCCGGCTA+CGG + chr8.1:37706614-37706633 MS.gene60993:CDS 45.0%
!! TCAGGGTATTGAAATGGTGG+AGG - chr8.1:37706153-37706172 None:intergenic 45.0%
GATCGGAAGGTCAGTGTCTA+TGG + chr8.1:37706818-37706837 MS.gene60993:CDS 50.0%
!! GGGTATTGAAATGGTGGAGG+TGG - chr8.1:37706150-37706169 None:intergenic 50.0%
! TGAAATGGTGGAGGTGGTGG+AGG - chr8.1:37706144-37706163 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 37706030 37707097 37706030 ID=MS.gene60993
chr8.1 mRNA 37706030 37707097 37706030 ID=MS.gene60993.t1;Parent=MS.gene60993
chr8.1 exon 37706030 37707097 37706030 ID=MS.gene60993.t1.exon1;Parent=MS.gene60993.t1
chr8.1 CDS 37706030 37707097 37706030 ID=cds.MS.gene60993.t1;Parent=MS.gene60993.t1
Gene Sequence

>MS.gene60993

ATGTCGCGTCCCAATTATCCAACAACATGGATTCCTTACATAAACAGCAAAGATTGTTCTCAAGGATTTTGTAGCGTGTATTGTCCACAATGGTGCTATATAGTTTACACTCCTCCACCACCTCCACCATTTCAATACCCTGATGATGATTCAAAACCATATTTCTCTCCTCTTGTTATTGCAATCTTGGGAGTTTTAGCCACTGCTTTTTTACTACTAACTTATTACACACTAATATCTAAATATTGTGGTCACAGAGAATCTACAAGAAGAAACACCACTGACCCAGTTGATGAAATCCAACAAAATCGCCGCGAAAACTGTCAAGTTTCAACCTTTGGTTTAGATGATGCTCAAATAAAATCCATTGCAGTTTTTAAATACAAAAAAGGAGATGCTTTTTTTAGTACTGCTGTCACAGATTGTTCAGTTTGTTTAAGTGAGTTTCAAGATGATGAAAGTGTTAGACTTTTACCAATGTGTAACCATGTTTTTCATCTTCCTTGTATTGATACATGGCTCAAATCTAACTCTAGTTGTCCTCTATGTCGTTCTAGCGTATTCACTTTGAATGCTTCAACACATCATGTTCAAGTTCCGGCTACGGTTATAGAACTTCCTTCAAGAAATGAAACTTTTCAAGAAGAGCAACAAATAGTTGTGGAAGAAGCAATGATGCATCATAGTAGAGTTGAGTCGAAGGCGCCTTCATTGCGCGCTTTTAGTGATTTATGTAATTTGCAAGGAAGAGAAAGAATAATTGAAATTAGAGATGAAGTTTGTGAATCGATCGGAAGGTCAGTGTCTATGGATCATTCATTTCAAAATGGTTTTTTAATTGGTGATGTGTTGGATATGAATGAAGATGAAGATTTTTGTGAAGAAGGATGTTCAATGGATGTTGGTTCATCAAAGATATCACAAGGAGAAAGTAGTAAATCAAGGTACAAAAGAAGAGTGTTGCATTGTGTTATGAGTCCAATTGCAATGAAGAGATCGTTTTCTAATGGAAGATTTTCACTTGGTAAAATTGATAGAGGAAAAAGGCAAGGAATTTTGCCTGTTTGA
Protein sequence

>MS.gene60993.t1

MSRPNYPTTWIPYINSKDCSQGFCSVYCPQWCYIVYTPPPPPPFQYPDDDSKPYFSPLVIAILGVLATAFLLLTYYTLISKYCGHRESTRRNTTDPVDEIQQNRRENCQVSTFGLDDAQIKSIAVFKYKKGDAFFSTAVTDCSVCLSEFQDDESVRLLPMCNHVFHLPCIDTWLKSNSSCPLCRSSVFTLNASTHHVQVPATVIELPSRNETFQEEQQIVVEEAMMHHSRVESKAPSLRAFSDLCNLQGRERIIEIRDEVCESIGRSVSMDHSFQNGFLIGDVLDMNEDEDFCEEGCSMDVGSSKISQGESSKSRYKRRVLHCVMSPIAMKRSFSNGRFSLGKIDRGKRQGILPV