Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61166.t1 | XP_003591973.1 | 99.2 | 122 | 1 | 0 | 1 | 122 | 1 | 122 | 2.40E-56 | 228 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61166.t1 | Q9LU46 | 74.4 | 121 | 29 | 1 | 1 | 121 | 5 | 123 | 7.7e-40 | 164.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61166.t1 | G7I9M7 | 99.2 | 122 | 1 | 0 | 1 | 122 | 1 | 122 | 1.7e-56 | 228.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049801 | MS.gene61166 | 0.803445 | 3.47E-49 | -1.69E-46 |
| MS.gene050361 | MS.gene61166 | 0.811473 | 6.88E-51 | -1.69E-46 |
| MS.gene050646 | MS.gene61166 | 0.810481 | 1.13E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene61166 | 0.800234 | 1.59E-48 | -1.69E-46 |
| MS.gene058010 | MS.gene61166 | 0.803511 | 3.37E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene61166 | 0.815608 | 8.47E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61166.t1 | MTR_1g095810 | 98.374 | 123 | 2 | 0 | 1 | 123 | 1 | 123 | 9.59e-79 | 243 |
| MS.gene61166.t1 | MTR_1g026830 | 90.083 | 121 | 11 | 1 | 3 | 123 | 6 | 125 | 3.64e-68 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61166.t1 | AT5G51280 | 74.380 | 121 | 29 | 1 | 1 | 121 | 5 | 123 | 4.61e-53 | 175 |
| MS.gene61166.t1 | AT4G33370 | 38.017 | 121 | 28 | 1 | 1 | 121 | 1 | 74 | 1.47e-17 | 77.4 |
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATACTGAGCCTTTGCCTAC+TGG | 0.358544 | 1.4:-74523480 | MS.gene61166:CDS |
| AGCTTCCATGGCACGACGCT+TGG | 0.384004 | 1.4:+74523740 | None:intergenic |
| TAAGCCAAGTCTACTTGTTA+AGG | 0.431259 | 1.4:-74523637 | MS.gene61166:CDS |
| ATACTGAGCCTTTGCCTACT+GGG | 0.441611 | 1.4:-74523479 | MS.gene61166:CDS |
| ATGGCACGACGCTTGGCCAC+AGG | 0.447691 | 1.4:+74523747 | None:intergenic |
| GGAAGAAGAAGATGATTATG+AGG | 0.447944 | 1.4:-74523778 | MS.gene61166:CDS |
| CTAATTCACGAACCGACATA+AGG | 0.449435 | 1.4:+74523517 | None:intergenic |
| TAATTCACGAACCGACATAA+GGG | 0.473252 | 1.4:+74523518 | None:intergenic |
| CTCAGTATAAGTAATCCCTT+TGG | 0.481450 | 1.4:+74523494 | None:intergenic |
| TGTTCTGTGACACTGATCTC+AGG | 0.482801 | 1.4:+74523591 | None:intergenic |
| GGAGATGATTGAGAATTTGT+CGG | 0.499681 | 1.4:-74523550 | MS.gene61166:CDS |
| TGTGGCCAAGCGTCGTGCCA+TGG | 0.505463 | 1.4:-74523745 | MS.gene61166:CDS |
| TAGAATCTTCTGAGCTTCCA+TGG | 0.542080 | 1.4:+74523728 | None:intergenic |
| AGAAGATTCTACAACGCAAA+GGG | 0.543952 | 1.4:-74523716 | MS.gene61166:CDS |
| CGGTTCGTGAATTAGCCAAA+GGG | 0.544464 | 1.4:-74523509 | MS.gene61166:CDS |
| TATAAGAAGGATGTCGAAGA+AGG | 0.546105 | 1.4:-74523439 | MS.gene61166:CDS |
| ACAGATTGTTCAACAAGAGA+AGG | 0.551879 | 1.4:-74523571 | MS.gene61166:CDS |
| AGCCTCCTTTGCATATAAGA+AGG | 0.556618 | 1.4:-74523452 | MS.gene61166:CDS |
| GACATCCTTCTTATATGCAA+AGG | 0.558548 | 1.4:+74523447 | None:intergenic |
| GATGCCTTAACAAGTAGACT+TGG | 0.560699 | 1.4:+74523633 | None:intergenic |
| GGCTTCCACCCAGTAGGCAA+AGG | 0.565662 | 1.4:+74523471 | None:intergenic |
| TGGCACGACGCTTGGCCACA+GGG | 0.573527 | 1.4:+74523748 | None:intergenic |
| CAGAAGATTCTACAACGCAA+AGG | 0.606512 | 1.4:-74523717 | MS.gene61166:CDS |
| TCGGTTCGTGAATTAGCCAA+AGG | 0.607239 | 1.4:-74523510 | MS.gene61166:CDS |
| ATCCTTCTTATATGCAAAGG+AGG | 0.611900 | 1.4:+74523450 | None:intergenic |
| ATCATCTTCTTCGATTGCAG+CGG | 0.619028 | 1.4:+74523686 | None:intergenic |
| GGATAAGAAAACCCTTATGT+CGG | 0.648731 | 1.4:-74523529 | MS.gene61166:CDS |
| CTGAGCCTTTGCCTACTGGG+TGG | 0.663961 | 1.4:-74523476 | MS.gene61166:CDS |
| AAAGGAGGCTTCCACCCAGT+AGG | 0.671260 | 1.4:+74523465 | None:intergenic |
| TTATGAGGAGTATATCCCTG+TGG | 0.764837 | 1.4:-74523763 | MS.gene61166:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCAACGACTTTTAGTTTCT+CGG | + | chr1.4:74523549-74523568 | None:intergenic | 30.0% |
| ACAGATTGTTCAACAAGAGA+AGG | - | chr1.4:74523637-74523656 | MS.gene61166:CDS | 35.0% | |
| AGAAGATTCTACAACGCAAA+GGG | - | chr1.4:74523492-74523511 | MS.gene61166:CDS | 35.0% | |
| ATCCTTCTTATATGCAAAGG+AGG | + | chr1.4:74523761-74523780 | None:intergenic | 35.0% | |
| CTCAGTATAAGTAATCCCTT+TGG | + | chr1.4:74523717-74523736 | None:intergenic | 35.0% | |
| GACATCCTTCTTATATGCAA+AGG | + | chr1.4:74523764-74523783 | None:intergenic | 35.0% | |
| GGAAGAAGAAGATGATTATG+AGG | - | chr1.4:74523430-74523449 | MS.gene61166:CDS | 35.0% | |
| GGAGATGATTGAGAATTTGT+CGG | - | chr1.4:74523658-74523677 | MS.gene61166:CDS | 35.0% | |
| GGATAAGAAAACCCTTATGT+CGG | - | chr1.4:74523679-74523698 | MS.gene61166:CDS | 35.0% | |
| TAATTCACGAACCGACATAA+GGG | + | chr1.4:74523693-74523712 | None:intergenic | 35.0% | |
| TATAAGAAGGATGTCGAAGA+AGG | - | chr1.4:74523769-74523788 | MS.gene61166:CDS | 35.0% | |
| ! | TAAGCCAAGTCTACTTGTTA+AGG | - | chr1.4:74523571-74523590 | MS.gene61166:CDS | 35.0% |
| AGCCTCCTTTGCATATAAGA+AGG | - | chr1.4:74523756-74523775 | MS.gene61166:CDS | 40.0% | |
| ATCATCTTCTTCGATTGCAG+CGG | + | chr1.4:74523525-74523544 | None:intergenic | 40.0% | |
| CAGAAGATTCTACAACGCAA+AGG | - | chr1.4:74523491-74523510 | MS.gene61166:CDS | 40.0% | |
| CTAATTCACGAACCGACATA+AGG | + | chr1.4:74523694-74523713 | None:intergenic | 40.0% | |
| GATGCCTTAACAAGTAGACT+TGG | + | chr1.4:74523578-74523597 | None:intergenic | 40.0% | |
| TAGAATCTTCTGAGCTTCCA+TGG | + | chr1.4:74523483-74523502 | None:intergenic | 40.0% | |
| ! | TTATGAGGAGTATATCCCTG+TGG | - | chr1.4:74523445-74523464 | MS.gene61166:CDS | 40.0% |
| ATACTGAGCCTTTGCCTACT+GGG | - | chr1.4:74523729-74523748 | MS.gene61166:CDS | 45.0% | |
| CGGTTCGTGAATTAGCCAAA+GGG | - | chr1.4:74523699-74523718 | MS.gene61166:CDS | 45.0% | |
| TATACTGAGCCTTTGCCTAC+TGG | - | chr1.4:74523728-74523747 | MS.gene61166:CDS | 45.0% | |
| TGTTCTGTGACACTGATCTC+AGG | + | chr1.4:74523620-74523639 | None:intergenic | 45.0% | |
| ! | TCGGTTCGTGAATTAGCCAA+AGG | - | chr1.4:74523698-74523717 | MS.gene61166:CDS | 45.0% |
| AAAGGAGGCTTCCACCCAGT+AGG | + | chr1.4:74523746-74523765 | None:intergenic | 55.0% | |
| AGCTTCCATGGCACGACGCT+TGG | + | chr1.4:74523471-74523490 | None:intergenic | 60.0% | |
| CTGAGCCTTTGCCTACTGGG+TGG | - | chr1.4:74523732-74523751 | MS.gene61166:CDS | 60.0% | |
| GGCTTCCACCCAGTAGGCAA+AGG | + | chr1.4:74523740-74523759 | None:intergenic | 60.0% | |
| ATGGCACGACGCTTGGCCAC+AGG | + | chr1.4:74523464-74523483 | None:intergenic | 65.0% | |
| TGGCACGACGCTTGGCCACA+GGG | + | chr1.4:74523463-74523482 | None:intergenic | 65.0% | |
| ! | TGTGGCCAAGCGTCGTGCCA+TGG | - | chr1.4:74523463-74523482 | MS.gene61166:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 74523428 | 74523802 | 74523428 | ID=MS.gene61166 |
| chr1.4 | mRNA | 74523428 | 74523802 | 74523428 | ID=MS.gene61166.t1;Parent=MS.gene61166 |
| chr1.4 | exon | 74523428 | 74523802 | 74523428 | ID=MS.gene61166.t1.exon1;Parent=MS.gene61166.t1 |
| chr1.4 | CDS | 74523428 | 74523802 | 74523428 | ID=cds.MS.gene61166.t1;Parent=MS.gene61166.t1 |
| Gene Sequence |
| Protein sequence |
