Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61245.t1 | XP_013457554.1 | 95.2 | 290 | 14 | 0 | 6 | 295 | 70 | 359 | 9.40E-128 | 466.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61245.t1 | A0A072V0D3 | 95.2 | 290 | 14 | 0 | 6 | 295 | 70 | 359 | 6.8e-128 | 466.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05188 | MS.gene61245 | 0.80194 | 7.10E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene61245 | 0.814037 | 1.89E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene61245 | 0.821469 | 3.97E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61245.t1 | MTR_4g099070 | 96.907 | 291 | 9 | 0 | 5 | 295 | 69 | 359 | 0.0 | 570 |
| MS.gene61245.t1 | MTR_4g099070 | 96.564 | 291 | 9 | 1 | 5 | 295 | 69 | 358 | 0.0 | 564 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61245.t1 | AT1G32730 | 42.177 | 294 | 140 | 6 | 5 | 295 | 61 | 327 | 1.44e-59 | 193 |
Find 46 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAGGACCCAACCAATTT+TGG | 0.167134 | 8.2:-23061101 | None:intergenic |
| GGATTACTCGTTACCAAAAT+TGG | 0.199926 | 8.2:+23061088 | MS.gene61245:CDS |
| GCATGTACCTTTCTAGTTAG+TGG | 0.276012 | 8.2:-23060400 | None:intergenic |
| CATGCAAAATGTAGATTTGC+TGG | 0.332643 | 8.2:+23060605 | MS.gene61245:CDS |
| ATCAGCTATAAGTGATCTTC+TGG | 0.345352 | 8.2:+23060902 | MS.gene61245:CDS |
| TACTTGTAACTTACATTGAC+GGG | 0.360139 | 8.2:-23055497 | None:intergenic |
| CTCCTTGTATTCCTTTAACT+TGG | 0.360826 | 8.2:-23060544 | None:intergenic |
| CTGCAAATCCTCCTCACTTC+GGG | 0.374251 | 8.2:-23060940 | None:intergenic |
| ATTGAACTGAGCAAAACTAT+TGG | 0.381132 | 8.2:-23060347 | None:intergenic |
| CCTGACAACACTCCATCTTC+TGG | 0.385227 | 8.2:+23055438 | MS.gene61245:CDS |
| TGTTGTACCAGCAAAAGAAT+TGG | 0.415028 | 8.2:+23061067 | MS.gene61245:CDS |
| CTGGGTTAAAATTGCAACTA+AGG | 0.442245 | 8.2:+23060721 | MS.gene61245:CDS |
| CCAGAAGATGGAGTGTTGTC+AGG | 0.449973 | 8.2:-23055438 | None:intergenic |
| AACAATGAGGTAATGATCCC+TGG | 0.455916 | 8.2:+23060702 | MS.gene61245:CDS |
| ACTCGTTACCAAAATTGGTT+GGG | 0.456983 | 8.2:+23061093 | MS.gene61245:CDS |
| AAGCTCAAGTCTGCTGTAGA+TGG | 0.465375 | 8.2:+23060810 | MS.gene61245:CDS |
| ATACTTGTAACTTACATTGA+CGG | 0.473704 | 8.2:-23055498 | None:intergenic |
| TACTCGTTACCAAAATTGGT+TGG | 0.478001 | 8.2:+23061092 | MS.gene61245:CDS |
| GGAGGATTTGCAGTCATGCT+TGG | 0.486799 | 8.2:+23060950 | MS.gene61245:CDS |
| AATCTTCGAATAAGAGAAAG+AGG | 0.496068 | 8.2:+23060868 | MS.gene61245:CDS |
| TTCCAAGTTAAAGGAATACA+AGG | 0.503227 | 8.2:+23060542 | MS.gene61245:CDS |
| ACTGCAAATCCTCCTCACTT+CGG | 0.516635 | 8.2:-23060941 | None:intergenic |
| AGTGGGGCAGCACCAGAAGA+TGG | 0.528171 | 8.2:-23055450 | None:intergenic |
| TACCTCATTGTTCTCTGTCG+AGG | 0.532314 | 8.2:-23060691 | None:intergenic |
| AAAGAAGCCACTAACTAGAA+AGG | 0.540503 | 8.2:+23060393 | MS.gene61245:CDS |
| GGATTTGCAGTCATGCTTGG+AGG | 0.551796 | 8.2:+23060953 | MS.gene61245:CDS |
| ATGCTGCAGCTATCAATGAA+TGG | 0.555763 | 8.2:+23060514 | MS.gene61245:CDS |
| TCATTGTTCTCTGTCGAGGT+AGG | 0.556432 | 8.2:-23060687 | None:intergenic |
| GAGAATGTAGAGCAGTTATG+AGG | 0.563614 | 8.2:+23061173 | MS.gene61245:CDS |
| CCGACGATGGCAGCAAAACA+AGG | 0.565614 | 8.2:+23052695 | None:intergenic |
| AGTGTTGTCAGGAATTGTAC+TGG | 0.583392 | 8.2:-23055427 | None:intergenic |
| TCAAACTCGACGAGAAGTGA+TGG | 0.595013 | 8.2:+23060744 | MS.gene61245:CDS |
| ACAATGAGGTAATGATCCCT+GGG | 0.596258 | 8.2:+23060703 | MS.gene61245:CDS |
| TGAGGATGTGTCTTCTGCAT+CGG | 0.596526 | 8.2:+23060656 | MS.gene61245:CDS |
| TTCAGACGAAGACTCTGCTA+TGG | 0.603260 | 8.2:+23060995 | MS.gene61245:CDS |
| CATCAGAATCAAACTGCTGA+AGG | 0.604583 | 8.2:+23061041 | MS.gene61245:CDS |
| AGTTTCTTGATCAATTTCTG+AGG | 0.608221 | 8.2:-23061117 | None:intergenic |
| ATCTGTGAAGCATTCAGATG+AGG | 0.622176 | 8.2:+23060638 | MS.gene61245:CDS |
| TAACAAAGCCCGAAGTGAGG+AGG | 0.633319 | 8.2:+23060932 | MS.gene61245:CDS |
| CAAGGAGCGAAATGTTGAAG+CGG | 0.634300 | 8.2:+23060560 | MS.gene61245:CDS |
| GGATGTGTCTTCTGCATCGG+AGG | 0.640331 | 8.2:+23060659 | MS.gene61245:CDS |
| ACTTGTAACTTACATTGACG+GGG | 0.642253 | 8.2:-23055496 | None:intergenic |
| AATTAACAAAGCCCGAAGTG+AGG | 0.646396 | 8.2:+23060929 | MS.gene61245:CDS |
| CTTGTAACTTACATTGACGG+GGG | 0.680304 | 8.2:-23055495 | None:intergenic |
| CTGCAGCTATCAATGAATGG+AGG | 0.684130 | 8.2:+23060517 | MS.gene61245:CDS |
| TACCTCGACAGAGAACAATG+AGG | 0.769377 | 8.2:+23060689 | MS.gene61245:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 23052701 | 23061193 | 23052701 | ID=MS.gene61245 |
| chr8.2 | mRNA | 23052701 | 23061193 | 23052701 | ID=MS.gene61245.t1;Parent=MS.gene61245 |
| chr8.2 | exon | 23052701 | 23052716 | 23052701 | ID=MS.gene61245.t1.exon1;Parent=MS.gene61245.t1 |
| chr8.2 | CDS | 23052701 | 23052716 | 23052701 | ID=cds.MS.gene61245.t1;Parent=MS.gene61245.t1 |
| chr8.2 | exon | 23055418 | 23055505 | 23055418 | ID=MS.gene61245.t1.exon2;Parent=MS.gene61245.t1 |
| chr8.2 | CDS | 23055418 | 23055505 | 23055418 | ID=cds.MS.gene61245.t1;Parent=MS.gene61245.t1 |
| chr8.2 | exon | 23060312 | 23060414 | 23060312 | ID=MS.gene61245.t1.exon3;Parent=MS.gene61245.t1 |
| chr8.2 | CDS | 23060312 | 23060414 | 23060312 | ID=cds.MS.gene61245.t1;Parent=MS.gene61245.t1 |
| chr8.2 | exon | 23060513 | 23061193 | 23060513 | ID=MS.gene61245.t1.exon4;Parent=MS.gene61245.t1 |
| chr8.2 | CDS | 23060513 | 23061193 | 23060513 | ID=cds.MS.gene61245.t1;Parent=MS.gene61245.t1 |
| Gene Sequence |
| Protein sequence |
