Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61948.t1 | XP_003615387.1 | 96 | 124 | 5 | 0 | 1 | 124 | 1 | 124 | 1.60E-52 | 215.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61948.t1 | Q9LSK9 | 39.4 | 109 | 61 | 2 | 8 | 116 | 14 | 117 | 9.7e-14 | 77.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61948.t1 | B7FMD8 | 96.0 | 124 | 5 | 0 | 1 | 124 | 1 | 124 | 1.2e-52 | 215.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene61948 | 0.812021 | 5.23E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene61948 | 0.81318 | 2.92E-51 | -1.69E-46 |
| MS.gene053536 | MS.gene61948 | 0.829936 | 3.90E-55 | -1.69E-46 |
| MS.gene053538 | MS.gene61948 | 0.820878 | 5.43E-53 | -1.69E-46 |
| MS.gene057132 | MS.gene61948 | 0.818804 | 1.62E-52 | -1.69E-46 |
| MS.gene05894 | MS.gene61948 | 0.83123 | 1.88E-55 | -1.69E-46 |
| MS.gene059424 | MS.gene61948 | 0.814665 | 1.37E-51 | -1.69E-46 |
| MS.gene060962 | MS.gene61948 | 0.840223 | 9.89E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61948.t1 | MTR_5g067320 | 95.968 | 124 | 5 | 0 | 1 | 124 | 1 | 124 | 3.69e-75 | 219 |
| MS.gene61948.t1 | MTR_3g111270 | 57.009 | 107 | 40 | 2 | 1 | 105 | 1 | 103 | 5.72e-23 | 86.7 |
| MS.gene61948.t1 | MTR_8g067590 | 35.200 | 125 | 65 | 3 | 1 | 118 | 1 | 116 | 3.56e-19 | 77.4 |
| MS.gene61948.t1 | MTR_5g030740 | 37.273 | 110 | 66 | 2 | 9 | 118 | 17 | 123 | 6.46e-15 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61948.t1 | AT5G65660 | 40.909 | 110 | 60 | 2 | 8 | 117 | 14 | 118 | 3.22e-23 | 88.2 |
Find 22 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCCATGGCTATGAATTT+TGG | 0.222092 | 5.2:-57313046 | None:intergenic |
| TTAATGACCCCAGAAGAATA+TGG | 0.406197 | 5.2:-57312297 | None:intergenic |
| TTGTTTCTTCCTTGTGCACA+TGG | 0.427036 | 5.2:-57313105 | None:intergenic |
| ATAACCATTGGTCTTCCATT+GGG | 0.430090 | 5.2:+57312189 | MS.gene61948:CDS |
| AATAACCATTGGTCTTCCAT+TGG | 0.431356 | 5.2:+57312188 | MS.gene61948:CDS |
| GTGATACTTTCATCTGTTGG+AGG | 0.442771 | 5.2:-57313079 | None:intergenic |
| TCATCTGTTGGAGGTTTACA+TGG | 0.459367 | 5.2:-57313070 | None:intergenic |
| GGGAAGATAATTACCACAAA+AGG | 0.461341 | 5.2:-57312375 | None:intergenic |
| ATTACCACAAAAGGAAATGC+TGG | 0.491723 | 5.2:-57312366 | None:intergenic |
| ATTGTGATACTTTCATCTGT+TGG | 0.494711 | 5.2:-57313082 | None:intergenic |
| AGCTTGCCTGTGTTGATGCC+AGG | 0.530092 | 5.2:+57313013 | MS.gene61948:CDS |
| AAAGGAAATGCTGGTTTCTG+AGG | 0.551783 | 5.2:-57312357 | None:intergenic |
| TTTGTTTCCATATTCTTCTG+GGG | 0.556303 | 5.2:+57312290 | MS.gene61948:CDS |
| GTGCACAAGGAAGAAACAAA+TGG | 0.558777 | 5.2:+57313109 | MS.gene61948:CDS |
| GCAGCAACTAAAATAACCAT+TGG | 0.572179 | 5.2:+57312177 | MS.gene61948:CDS |
| GATCCCAAAATTCATAGCCA+TGG | 0.591806 | 5.2:+57313042 | MS.gene61948:CDS |
| TATCACAATCCATGTGCACA+AGG | 0.596792 | 5.2:+57313096 | MS.gene61948:CDS |
| AGGTTTACATGGACATGCCA+TGG | 0.597625 | 5.2:-57313059 | None:intergenic |
| TCATCTCCTGGCATCAACAC+AGG | 0.605248 | 5.2:-57313019 | None:intergenic |
| AAGAGAAGTGCTAAACCCAA+TGG | 0.608171 | 5.2:-57312204 | None:intergenic |
| AAATGCTGGTTTCTGAGGAG+AGG | 0.637186 | 5.2:-57312352 | None:intergenic |
| TAAACCCAATGGAAGACCAA+TGG | 0.650172 | 5.2:-57312193 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACAAAAAAAAACTAGTTA+AGG | - | chr5.2:57312610-57312629 | None:intergenic | 15.0% |
| !! | TATATATATACACACATAAT+TGG | + | chr5.2:57312922-57312941 | MS.gene61948:intron | 15.0% |
| !!! | GTAGTACTAAATAATTTTTT+TGG | + | chr5.2:57312441-57312460 | MS.gene61948:intron | 15.0% |
| !! | AAAAAAAAACTAGTTAAGGA+AGG | - | chr5.2:57312606-57312625 | None:intergenic | 20.0% |
| !! | ACAAAATTACACAGAATAAA+AGG | + | chr5.2:57312819-57312838 | MS.gene61948:intron | 20.0% |
| !! | CAAAATTACACAGAATAAAA+GGG | + | chr5.2:57312820-57312839 | MS.gene61948:intron | 20.0% |
| !! | TATTTAGTGAGAAATAACAT+GGG | - | chr5.2:57312398-57312417 | None:intergenic | 20.0% |
| !! | TTATTTAGTGAGAAATAACA+TGG | - | chr5.2:57312399-57312418 | None:intergenic | 20.0% |
| !!! | AGATTGATTGTATTGTTTTT+TGG | + | chr5.2:57312959-57312978 | MS.gene61948:intron | 20.0% |
| ! | ATATATACACACATAATTGG+AGG | + | chr5.2:57312925-57312944 | MS.gene61948:intron | 25.0% |
| !!! | TCTTTTGTTGCTTATTACAT+TGG | + | chr5.2:57312253-57312272 | MS.gene61948:CDS | 25.0% |
| !!! | TTTTGTTTCCATATTCTTCT+GGG | + | chr5.2:57312289-57312308 | MS.gene61948:CDS | 25.0% |
| !!! | TTTTTGTTTCCATATTCTTC+TGG | + | chr5.2:57312288-57312307 | MS.gene61948:CDS | 25.0% |
| ATTGTGATACTTTCATCTGT+TGG | - | chr5.2:57313085-57313104 | None:intergenic | 30.0% | |
| TTTGTTTCCATATTCTTCTG+GGG | + | chr5.2:57312290-57312309 | MS.gene61948:CDS | 30.0% | |
| !!! | TGTATTGTTTTTTGGTAAGC+AGG | + | chr5.2:57312967-57312986 | MS.gene61948:intron | 30.0% |
| ATTACCACAAAAGGAAATGC+TGG | - | chr5.2:57312369-57312388 | None:intergenic | 35.0% | |
| GCAGCAACTAAAATAACCAT+TGG | + | chr5.2:57312177-57312196 | MS.gene61948:CDS | 35.0% | |
| GGGAAGATAATTACCACAAA+AGG | - | chr5.2:57312378-57312397 | None:intergenic | 35.0% | |
| TTAATGACCCCAGAAGAATA+TGG | - | chr5.2:57312300-57312319 | None:intergenic | 35.0% | |
| ! | ATGCCATGGCTATGAATTTT+GGG | - | chr5.2:57313048-57313067 | None:intergenic | 35.0% |
| !! | AATAACCATTGGTCTTCCAT+TGG | + | chr5.2:57312188-57312207 | MS.gene61948:CDS | 35.0% |
| !! | ATAACCATTGGTCTTCCATT+GGG | + | chr5.2:57312189-57312208 | MS.gene61948:CDS | 35.0% |
| GATCCCAAAATTCATAGCCA+TGG | + | chr5.2:57313042-57313061 | MS.gene61948:CDS | 40.0% | |
| GTGATACTTTCATCTGTTGG+AGG | - | chr5.2:57313082-57313101 | None:intergenic | 40.0% | |
| GTGCACAAGGAAGAAACAAA+TGG | + | chr5.2:57313109-57313128 | MS.gene61948:CDS | 40.0% | |
| TAAACCCAATGGAAGACCAA+TGG | - | chr5.2:57312196-57312215 | None:intergenic | 40.0% | |
| TATCACAATCCATGTGCACA+AGG | + | chr5.2:57313096-57313115 | MS.gene61948:CDS | 40.0% | |
| TCATCTGTTGGAGGTTTACA+TGG | - | chr5.2:57313073-57313092 | None:intergenic | 40.0% | |
| ! | CATGCCATGGCTATGAATTT+TGG | - | chr5.2:57313049-57313068 | None:intergenic | 40.0% |
| ! | GAAACCAGCATTTCCTTTTG+TGG | + | chr5.2:57312362-57312381 | MS.gene61948:CDS | 40.0% |
| ! | TTGTTTCTTCCTTGTGCACA+TGG | - | chr5.2:57313108-57313127 | None:intergenic | 40.0% |
| !! | AAAGGAAATGCTGGTTTCTG+AGG | - | chr5.2:57312360-57312379 | None:intergenic | 40.0% |
| !! | AAGAGAAGTGCTAAACCCAA+TGG | - | chr5.2:57312207-57312226 | None:intergenic | 40.0% |
| !! | ACTAGTTAAGGAAGGCACTA+AGG | - | chr5.2:57312598-57312617 | None:intergenic | 40.0% |
| !!! | AATTTTGGGATCTCATCTCC+TGG | - | chr5.2:57313034-57313053 | None:intergenic | 40.0% |
| AGGTTTACATGGACATGCCA+TGG | - | chr5.2:57313062-57313081 | None:intergenic | 45.0% | |
| !! | AAATGCTGGTTTCTGAGGAG+AGG | - | chr5.2:57312355-57312374 | None:intergenic | 45.0% |
| TCATCTCCTGGCATCAACAC+AGG | - | chr5.2:57313022-57313041 | None:intergenic | 50.0% | |
| ! | AGCTTGCCTGTGTTGATGCC+AGG | + | chr5.2:57313013-57313032 | MS.gene61948:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 57312159 | 57313144 | 57312159 | ID=MS.gene61948 |
| chr5.2 | mRNA | 57312159 | 57313144 | 57312159 | ID=MS.gene61948.t1;Parent=MS.gene61948 |
| chr5.2 | exon | 57312159 | 57312383 | 57312159 | ID=MS.gene61948.t1.exon1;Parent=MS.gene61948.t1 |
| chr5.2 | CDS | 57312159 | 57312383 | 57312159 | ID=cds.MS.gene61948.t1;Parent=MS.gene61948.t1 |
| chr5.2 | exon | 57312989 | 57313144 | 57312989 | ID=MS.gene61948.t1.exon2;Parent=MS.gene61948.t1 |
| chr5.2 | CDS | 57312989 | 57313144 | 57312989 | ID=cds.MS.gene61948.t1;Parent=MS.gene61948.t1 |
| Gene Sequence |
| Protein sequence |
