Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62454.t1 | XP_003609265.1 | 99.1 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 8.60E-47 | 196.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62454.t1 | Q02921 | 84.8 | 99 | 15 | 0 | 9 | 107 | 5 | 103 | 1.8e-37 | 156.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62454.t1 | G7JVA6 | 99.1 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 6.2e-47 | 196.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055157 | MS.gene62454 | 0.85548 | 6.13E-62 | -1.69E-46 |
| MS.gene055601 | MS.gene62454 | 0.80404 | 2.61E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene62454 | 0.807641 | 4.58E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62454.t1 | MTR_4g113820 | 99.065 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 1.75e-72 | 211 |
| MS.gene62454.t1 | MTR_1g007360 | 60.759 | 79 | 31 | 0 | 21 | 99 | 26 | 104 | 7.36e-29 | 101 |
| MS.gene62454.t1 | MTR_3g110390 | 53.086 | 81 | 38 | 0 | 21 | 101 | 27 | 107 | 1.56e-25 | 92.8 |
| MS.gene62454.t1 | MTR_3g110390 | 51.852 | 81 | 38 | 1 | 21 | 101 | 27 | 106 | 8.87e-23 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62454.t1 | AT5G25940 | 67.347 | 49 | 16 | 0 | 52 | 100 | 55 | 103 | 9.73e-18 | 72.8 |
Find 31 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACCAGCTTGGCTTATC+TGG | 0.217498 | 8.2:-14480484 | MS.gene62454:CDS |
| GAGCTTGAGCAGTGGGATTC+AGG | 0.269132 | 8.2:+14480068 | None:intergenic |
| TGCATTGGAGGGTCGGTTGA+TGG | 0.374033 | 8.2:+14479871 | None:intergenic |
| TGCATTTGTAATTCATGCAT+TGG | 0.411064 | 8.2:+14479856 | None:intergenic |
| CTAGTGTTAGGATGCTACCT+TGG | 0.442749 | 8.2:-14480102 | MS.gene62454:CDS |
| CAAGCTCTCATAATTTCTAC+AGG | 0.456647 | 8.2:-14480052 | MS.gene62454:intron |
| ATTAGCAGCAACATTTCTCT+TGG | 0.469394 | 8.2:+14480518 | None:intergenic |
| GCTTGGCCATGGCCAACCTC+TGG | 0.472159 | 8.2:+14480460 | None:intergenic |
| ACATGCAGCTGGCTAGTGTT+AGG | 0.477537 | 8.2:-14480114 | MS.gene62454:intron |
| GGCTTATCTGGACCAGAGGT+TGG | 0.484520 | 8.2:-14480472 | MS.gene62454:CDS |
| TCCTCTTGAGAGAACCAGCT+TGG | 0.495079 | 8.2:-14480493 | MS.gene62454:CDS |
| ATGAGAACAGCGCTTGGCCA+TGG | 0.500574 | 8.2:+14480449 | None:intergenic |
| AGTAGCTGACAAGACTGTAT+TGG | 0.503109 | 8.2:-14479912 | MS.gene62454:CDS |
| GGGTGTAATGGCTGGAGCTA+AGG | 0.508105 | 8.2:-14480331 | MS.gene62454:CDS |
| TTCAGGTTTGCTCTTGCCCA+AGG | 0.514820 | 8.2:+14480085 | None:intergenic |
| TAGTGTTAGGATGCTACCTT+GGG | 0.518544 | 8.2:-14480101 | MS.gene62454:CDS |
| ATGGCCAAGCGCTGTTCTCA+TGG | 0.525880 | 8.2:-14480447 | MS.gene62454:intron |
| TAATTCATGCATTGGAGGGT+CGG | 0.526783 | 8.2:+14479864 | None:intergenic |
| ATTATGAGAGCTTGAGCAGT+GGG | 0.542769 | 8.2:+14480061 | None:intergenic |
| CTGGTCCAGATAAGCCAAGC+TGG | 0.549243 | 8.2:+14480479 | None:intergenic |
| ATTTGTAATTCATGCATTGG+AGG | 0.558334 | 8.2:+14479859 | None:intergenic |
| AGGAGTAGTTACAGTTGGAA+TGG | 0.559635 | 8.2:+14480281 | None:intergenic |
| TAAACAGAGGGTGTAATGGC+TGG | 0.563650 | 8.2:-14480339 | MS.gene62454:intron |
| TTTGTAATTCATGCATTGGA+GGG | 0.572592 | 8.2:+14479860 | None:intergenic |
| TGTGTAAACAGAGGGTGTAA+TGG | 0.582073 | 8.2:-14480343 | MS.gene62454:intron |
| GCCAAGCTGGTTCTCTCAAG+AGG | 0.593616 | 8.2:+14480492 | None:intergenic |
| AGTTACAGTTGGAATGGCAG+TGG | 0.597023 | 8.2:+14480287 | None:intergenic |
| TCTGGACCAGAGGTTGGCCA+TGG | 0.615026 | 8.2:-14480466 | MS.gene62454:CDS |
| ATTACCATGAGAACAGCGCT+TGG | 0.632062 | 8.2:+14480443 | None:intergenic |
| GCTTGGCTTATCTGGACCAG+AGG | 0.644191 | 8.2:-14480476 | MS.gene62454:CDS |
| AATTATGAGAGCTTGAGCAG+TGG | 0.660731 | 8.2:+14480060 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACTAAATACAATAACAA+AGG | + | chr8.2:14480017-14480036 | None:intergenic | 15.0% |
| !! | CAGAACTATATCAAAATTAA+TGG | + | chr8.2:14480167-14480186 | None:intergenic | 20.0% |
| !! | TCAATGTATAATGTAATCTT+TGG | - | chr8.2:14479988-14480007 | MS.gene62454:intron | 20.0% |
| !! | TTTCTTAGGATATTTAAAAG+TGG | + | chr8.2:14480214-14480233 | None:intergenic | 20.0% |
| !!! | CTTTTTGGTATATAATTAAC+TGG | - | chr8.2:14480238-14480257 | MS.gene62454:intron | 20.0% |
| ! | TGAAGCAAAGAAAATTGAAT+AGG | + | chr8.2:14480139-14480158 | None:intergenic | 25.0% |
| !! | ATCCTAAGAAAGATACTTTT+TGG | - | chr8.2:14480223-14480242 | MS.gene62454:intron | 25.0% |
| !!! | TACCAAAAAGTATCTTTCTT+AGG | + | chr8.2:14480228-14480247 | None:intergenic | 25.0% |
| AAGAAAATTGAATAGGTGGA+AGG | + | chr8.2:14480132-14480151 | None:intergenic | 30.0% | |
| AGCAAAGAAAATTGAATAGG+TGG | + | chr8.2:14480136-14480155 | None:intergenic | 30.0% | |
| CCACTACAAACAAAATATGA+GGG | + | chr8.2:14480439-14480458 | None:intergenic | 30.0% | |
| ATTAGCAGCAACATTTCTCT+TGG | + | chr8.2:14479875-14479894 | None:intergenic | 35.0% | |
| CAAGCTCTCATAATTTCTAC+AGG | - | chr8.2:14480338-14480357 | MS.gene62454:intron | 35.0% | |
| GCCACTACAAACAAAATATG+AGG | + | chr8.2:14480440-14480459 | None:intergenic | 35.0% | |
| TGTATCAAGCAATGATTGTG+AGG | + | chr8.2:14480407-14480426 | None:intergenic | 35.0% | |
| ! | TTGATCAGTGTTGAGCAATA+AGG | + | chr8.2:14480377-14480396 | None:intergenic | 35.0% |
| !! | CATATTTTGTTTGTAGTGGC+TGG | - | chr8.2:14480440-14480459 | MS.gene62454:intron | 35.0% |
| !! | CCCTCATATTTTGTTTGTAG+TGG | - | chr8.2:14480436-14480455 | MS.gene62454:intron | 35.0% |
| AATTATGAGAGCTTGAGCAG+TGG | + | chr8.2:14480333-14480352 | None:intergenic | 40.0% | |
| AGGAGTAGTTACAGTTGGAA+TGG | + | chr8.2:14480112-14480131 | None:intergenic | 40.0% | |
| AGTAGCTGACAAGACTGTAT+TGG | - | chr8.2:14480478-14480497 | MS.gene62454:CDS | 40.0% | |
| ATATATGCTTGACATGCAGC+TGG | - | chr8.2:14480265-14480284 | MS.gene62454:intron | 40.0% | |
| ATTATGAGAGCTTGAGCAGT+GGG | + | chr8.2:14480332-14480351 | None:intergenic | 40.0% | |
| CCATTCCTTGTGTAAACAGA+GGG | - | chr8.2:14480039-14480058 | MS.gene62454:intron | 40.0% | |
| TAGTGTTAGGATGCTACCTT+GGG | - | chr8.2:14480289-14480308 | MS.gene62454:intron | 40.0% | |
| TCCATTCCTTGTGTAAACAG+AGG | - | chr8.2:14480038-14480057 | MS.gene62454:intron | 40.0% | |
| TTACACCCTCTGTTTACACA+AGG | + | chr8.2:14480047-14480066 | None:intergenic | 40.0% | |
| ! | ATCAGTGTTGAGCAATAAGG+AGG | + | chr8.2:14480374-14480393 | None:intergenic | 40.0% |
| ! | TGTGTAAACAGAGGGTGTAA+TGG | - | chr8.2:14480047-14480066 | MS.gene62454:intron | 40.0% |
| AGTTACAGTTGGAATGGCAG+TGG | + | chr8.2:14480106-14480125 | None:intergenic | 45.0% | |
| ATTACCATGAGAACAGCGCT+TGG | + | chr8.2:14479950-14479969 | None:intergenic | 45.0% | |
| CCCTCTGTTTACACAAGGAA+TGG | + | chr8.2:14480042-14480061 | None:intergenic | 45.0% | |
| CTAGTGTTAGGATGCTACCT+TGG | - | chr8.2:14480288-14480307 | MS.gene62454:intron | 45.0% | |
| GTGGAAGGAGTAGTTACAGT+TGG | + | chr8.2:14480117-14480136 | None:intergenic | 45.0% | |
| ! | TAAACAGAGGGTGTAATGGC+TGG | - | chr8.2:14480051-14480070 | MS.gene62454:intron | 45.0% |
| ACATGCAGCTGGCTAGTGTT+AGG | - | chr8.2:14480276-14480295 | MS.gene62454:intron | 50.0% | |
| GAGAACCAGCTTGGCTTATC+TGG | - | chr8.2:14479906-14479925 | MS.gene62454:CDS | 50.0% | |
| TCCTCTTGAGAGAACCAGCT+TGG | - | chr8.2:14479897-14479916 | MS.gene62454:CDS | 50.0% | |
| ! | TTCAGGTTTGCTCTTGCCCA+AGG | + | chr8.2:14480308-14480327 | None:intergenic | 50.0% |
| ATGAGAACAGCGCTTGGCCA+TGG | + | chr8.2:14479944-14479963 | None:intergenic | 55.0% | |
| CTGGTCCAGATAAGCCAAGC+TGG | + | chr8.2:14479914-14479933 | None:intergenic | 55.0% | |
| GAGCTTGAGCAGTGGGATTC+AGG | + | chr8.2:14480325-14480344 | None:intergenic | 55.0% | |
| GCTTGGCTTATCTGGACCAG+AGG | - | chr8.2:14479914-14479933 | MS.gene62454:CDS | 55.0% | |
| GGCTTATCTGGACCAGAGGT+TGG | - | chr8.2:14479918-14479937 | MS.gene62454:CDS | 55.0% | |
| GGGTGTAATGGCTGGAGCTA+AGG | - | chr8.2:14480059-14480078 | MS.gene62454:CDS | 55.0% | |
| TGCATTGGAGGGTCGGTTGA+TGG | + | chr8.2:14480522-14480541 | None:intergenic | 55.0% | |
| ! | ATGGCCAAGCGCTGTTCTCA+TGG | - | chr8.2:14479943-14479962 | MS.gene62454:intron | 55.0% |
| ! | GCCAAGCTGGTTCTCTCAAG+AGG | + | chr8.2:14479901-14479920 | None:intergenic | 55.0% |
| ! | TCTGGACCAGAGGTTGGCCA+TGG | - | chr8.2:14479924-14479943 | MS.gene62454:CDS | 60.0% |
| GCTTGGCCATGGCCAACCTC+TGG | + | chr8.2:14479933-14479952 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 14479868 | 14480544 | 14479868 | ID=MS.gene62454 |
| chr8.2 | mRNA | 14479868 | 14480544 | 14479868 | ID=MS.gene62454.t1;Parent=MS.gene62454 |
| chr8.2 | exon | 14480448 | 14480544 | 14480448 | ID=MS.gene62454.t1.exon1;Parent=MS.gene62454.t1 |
| chr8.2 | CDS | 14480448 | 14480544 | 14480448 | ID=cds.MS.gene62454.t1;Parent=MS.gene62454.t1 |
| chr8.2 | exon | 14480293 | 14480354 | 14480293 | ID=MS.gene62454.t1.exon2;Parent=MS.gene62454.t1 |
| chr8.2 | CDS | 14480293 | 14480354 | 14480293 | ID=cds.MS.gene62454.t1;Parent=MS.gene62454.t1 |
| chr8.2 | exon | 14480053 | 14480128 | 14480053 | ID=MS.gene62454.t1.exon3;Parent=MS.gene62454.t1 |
| chr8.2 | CDS | 14480053 | 14480128 | 14480053 | ID=cds.MS.gene62454.t1;Parent=MS.gene62454.t1 |
| chr8.2 | exon | 14479868 | 14479956 | 14479868 | ID=MS.gene62454.t1.exon4;Parent=MS.gene62454.t1 |
| chr8.2 | CDS | 14479868 | 14479956 | 14479868 | ID=cds.MS.gene62454.t1;Parent=MS.gene62454.t1 |
| Gene Sequence |
| Protein sequence |
