AlfalfaGEDB | MS.gene62493

M. sativa cultivar XinJiangDaYe / MS.gene62493

KEGG: K02935
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00542.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62493.t1	GAU35832.1	90.3	216	21	0	1	216	1	216	1.50E-98	369

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62493.t1	P53163	39.7	146	81	3	78	216	49	194	9.4e-17	88.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62493.t1	A0A2K3P7T6	90.3	216	21	0	1	216	1	216	1.1e-98	369.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene68085	MS.gene62493	PPI
MS.gene60517	MS.gene62493	PPI
MS.gene62493	MS.gene09988	PPI
MS.gene005792	MS.gene62493	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62493.t1	MTR_2g064300	96.759	216	7	0	1	216	1	216	6.55e-153	423
MS.gene62493.t1	MTR_7g053520	84.793	217	32	1	1	216	1	217	1.74e-121	343
MS.gene62493.t1	MTR_2g031760	40.000	165	94	3	53	216	30	190	1.07e-32	117
MS.gene62493.t1	MTR_2g031960	47.143	140	67	3	79	216	21	155	1.73e-30	110
MS.gene62493.t1	MTR_8g076780	44.737	152	74	2	74	216	45	195	9.41e-30	109

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62493.t1	AT1G70190	53.636	220	86	3	1	216	1	208	1.17e-66	204
MS.gene62493.t1	AT1G70190	53.636	220	86	3	1	216	1	208	1.17e-66	204
MS.gene62493.t1	AT4G36420	43.684	190	92	4	30	216	2	179	1.01e-33	119
MS.gene62493.t1	AT3G06040	40.940	149	81	1	68	216	45	186	1.13e-27	103
MS.gene62493.t1	AT3G06040	40.940	149	81	1	68	216	45	186	1.13e-27	103
MS.gene62493.t1	AT3G06040	40.940	149	81	1	68	216	45	186	1.13e-27	103
MS.gene62493.t1	AT4G37660	46.212	132	63	1	85	216	44	167	1.10e-24	95.9
MS.gene62493.t1	AT2G03130	57.500	80	34	0	137	216	12	91	1.15e-15	70.5

Find 54 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTGATGACTTTAATTT+TGG	0.115213	2.1:-31355626	None:intergenic
CCAGTTTATGAAAGCGTATT+AGG	0.115413	2.1:+31355202	MS.gene62493:CDS
TGGAGGGCCACGATTGTTAT+TGG	0.240332	2.1:-31355362	None:intergenic
GAAAGCAGGGTCTGCAAATT+TGG	0.254350	2.1:+31355507	MS.gene62493:CDS
GTGCGGGGCTTTACCGATTT+AGG	0.299345	2.1:+31355649	MS.gene62493:CDS
CCTAATACGCTTTCATAAAC+TGG	0.317127	2.1:-31355202	None:intergenic
TGAGCTTGCTGTTGAGAATA+AGG	0.322760	2.1:+31355149	MS.gene62493:CDS
GCTAGCTGAAGGTGCGGAAT+TGG	0.332644	2.1:+31355429	MS.gene62493:CDS
CAGTTTATGAAAGCGTATTA+GGG	0.345172	2.1:+31355203	MS.gene62493:CDS
TGCCTCCTTCAAACCTAAAT+CGG	0.380631	2.1:-31355662	None:intergenic
GGAGGGCCACGATTGTTATT+GGG	0.390326	2.1:-31355361	None:intergenic
GGAGGAAGACGTAGAGGAAG+TGG	0.397120	2.1:+31355288	MS.gene62493:CDS
ACTTGGTGCTAATGTTGTTT+TGG	0.411925	2.1:+31355771	MS.gene62493:CDS
CTAGCTGAAGGTGCGGAATT+GGG	0.422415	2.1:+31355430	MS.gene62493:CDS
TTATTGGGATCAAATGTTGC+AGG	0.430449	2.1:-31355346	None:intergenic
CTTTACCGATTTAGGTTTGA+AGG	0.453564	2.1:+31355657	MS.gene62493:CDS
TAAAGTCATCAAGGAGGTGC+GGG	0.464501	2.1:+31355633	MS.gene62493:CDS
GTCTGCAAATTTGGCCGGAA+TGG	0.467477	2.1:+31355516	MS.gene62493:CDS
GCTTTCATATATCCCACATT+GGG	0.475885	2.1:-31355490	None:intergenic
CTCCTCTTGGGCTGCAGTCG+TGG	0.476435	2.1:-31355548	None:intergenic
AAAGGTGTTTCAAAGGAAGA+AGG	0.492660	2.1:+31355721	MS.gene62493:CDS
GTTGCAGGATCAAAATCAGC+TGG	0.495579	2.1:-31355331	None:intergenic
TATAAAGAAAGGTGTTTCAA+AGG	0.503102	2.1:+31355714	MS.gene62493:CDS
ACACCGTCTATTATAAAGAA+AGG	0.512263	2.1:+31355703	MS.gene62493:CDS
TGCTTTCATATATCCCACAT+TGG	0.515923	2.1:-31355491	None:intergenic
GCGTATTAGGGCTTAATGCT+AGG	0.525999	2.1:+31355215	MS.gene62493:CDS
ACACCTTTCTTTATAATAGA+CGG	0.526861	2.1:-31355706	None:intergenic
TTCCAAAATTAAAGTCATCA+AGG	0.526956	2.1:+31355624	MS.gene62493:CDS
AATGTGGGATATATGAAAGC+AGG	0.529942	2.1:+31355493	MS.gene62493:CDS
AGCTGCAAGAAAAGAGGAGG+AGG	0.530708	2.1:+31355270	MS.gene62493:CDS
TTTCAGGCTTGTTGATGAAG+TGG	0.535175	2.1:+31355396	MS.gene62493:CDS
AGCCTGAAAACTCTCTCTGA+TGG	0.540150	2.1:-31355382	None:intergenic
GCAGGGTCTGCAAATTTGGC+CGG	0.545113	2.1:+31355511	MS.gene62493:CDS
AGAGGAGGAGGAAGACGTAG+AGG	0.548567	2.1:+31355282	MS.gene62493:CDS
TTTAGGTTTGAAGGAGGCAA+AGG	0.562989	2.1:+31355666	MS.gene62493:CDS
CTGAAAACTCTCTCTGATGG+AGG	0.566309	2.1:-31355379	None:intergenic
TGGTCAAGCTGCAAGAAAAG+AGG	0.567125	2.1:+31355264	MS.gene62493:CDS
GCGTCTCTAACGCTAGCTGA+AGG	0.607910	2.1:+31355418	MS.gene62493:CDS
GATAAAAGAAATGCCCAATG+TGG	0.608771	2.1:+31355477	MS.gene62493:CDS
TGAAAACTCTCTCTGATGGA+GGG	0.610138	2.1:-31355378	None:intergenic
TTAAAGTCATCAAGGAGGTG+CGG	0.612909	2.1:+31355632	MS.gene62493:CDS
ATAGAGAAAATGAAAGCACT+TGG	0.623385	2.1:+31355754	MS.gene62493:CDS
ATGTGGGATATATGAAAGCA+GGG	0.623877	2.1:+31355494	MS.gene62493:CDS
ATAAAAGAAATGCCCAATGT+GGG	0.625462	2.1:+31355478	MS.gene62493:CDS
TACCGATTTAGGTTTGAAGG+AGG	0.626116	2.1:+31355660	MS.gene62493:CDS
CACGACTGCAGCCCAAGAGG+AGG	0.628136	2.1:+31355549	MS.gene62493:CDS
TTGCAGGATCAAAATCAGCT+GGG	0.640796	2.1:-31355330	None:intergenic
AGCCACGACTGCAGCCCAAG+AGG	0.642383	2.1:+31355546	MS.gene62493:CDS
TCAAGCTGCAAGAAAAGAGG+AGG	0.642765	2.1:+31355267	MS.gene62493:CDS
ATAAGGCATCGTTTACACAA+TGG	0.647228	2.1:+31355166	MS.gene62493:CDS
AAAGTCATCAAGGAGGTGCG+GGG	0.667732	2.1:+31355634	MS.gene62493:CDS
TTTGATCCCAATAACAATCG+TGG	0.681002	2.1:+31355355	MS.gene62493:CDS
TCTAACGCTAGCTGAAGGTG+CGG	0.697298	2.1:+31355423	MS.gene62493:CDS
CAAAATTAAAGTCATCAAGG+AGG	0.704497	2.1:+31355627	MS.gene62493:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTCAAATACAGTTTTTTC+TGG	-	chr2.1:31355580-31355599	None:intergenic	20.0%
!	ACACCTTTCTTTATAATAGA+CGG	-	chr2.1:31355709-31355728	None:intergenic	25.0%
!	TTCCAAAATTAAAGTCATCA+AGG	+	chr2.1:31355624-31355643	MS.gene62493:CDS	25.0%
!!!	AATGTATTTTCGAGAAACTT+TGG	+	chr2.1:31355244-31355263	MS.gene62493:CDS	25.0%
!!!	CTCATTTTGATGAAGAAAAT+GGG	+	chr2.1:31355454-31355473	MS.gene62493:CDS	25.0%
!!!	TATAAAGAAAGGTGTTTCAA+AGG	+	chr2.1:31355714-31355733	MS.gene62493:CDS	25.0%
!!!	TCATTTTGATGAAGAAAATG+GGG	+	chr2.1:31355455-31355474	MS.gene62493:CDS	25.0%
!!!	TCTCATTTTGATGAAGAAAA+TGG	+	chr2.1:31355453-31355472	MS.gene62493:CDS	25.0%
	ACACCGTCTATTATAAAGAA+AGG	+	chr2.1:31355703-31355722	MS.gene62493:CDS	30.0%
	ATAAAAGAAATGCCCAATGT+GGG	+	chr2.1:31355478-31355497	MS.gene62493:CDS	30.0%
	CAAAATTAAAGTCATCAAGG+AGG	+	chr2.1:31355627-31355646	MS.gene62493:CDS	30.0%
!	CAGTTTATGAAAGCGTATTA+GGG	+	chr2.1:31355203-31355222	MS.gene62493:CDS	30.0%
!!	ATAGAGAAAATGAAAGCACT+TGG	+	chr2.1:31355754-31355773	MS.gene62493:CDS	30.0%
!!	CTCCTTGATGACTTTAATTT+TGG	-	chr2.1:31355629-31355648	None:intergenic	30.0%
	AATGTGGGATATATGAAAGC+AGG	+	chr2.1:31355493-31355512	MS.gene62493:CDS	35.0%
	ATGTGGGATATATGAAAGCA+GGG	+	chr2.1:31355494-31355513	MS.gene62493:CDS	35.0%
	CCTAATACGCTTTCATAAAC+TGG	-	chr2.1:31355205-31355224	None:intergenic	35.0%
	GATAAAAGAAATGCCCAATG+TGG	+	chr2.1:31355477-31355496	MS.gene62493:CDS	35.0%
	GCTTTCATATATCCCACATT+GGG	-	chr2.1:31355493-31355512	None:intergenic	35.0%
	TGCTTTCATATATCCCACAT+TGG	-	chr2.1:31355494-31355513	None:intergenic	35.0%
	TTATTGGGATCAAATGTTGC+AGG	-	chr2.1:31355349-31355368	None:intergenic	35.0%
	TTTGATCCCAATAACAATCG+TGG	+	chr2.1:31355355-31355374	MS.gene62493:CDS	35.0%
!	CCAGTTTATGAAAGCGTATT+AGG	+	chr2.1:31355202-31355221	MS.gene62493:CDS	35.0%
!!	AAAGGTGTTTCAAAGGAAGA+AGG	+	chr2.1:31355721-31355740	MS.gene62493:CDS	35.0%
!!	ATAAGGCATCGTTTACACAA+TGG	+	chr2.1:31355166-31355185	MS.gene62493:CDS	35.0%
!!	CTTTACCGATTTAGGTTTGA+AGG	+	chr2.1:31355657-31355676	MS.gene62493:CDS	35.0%
!!!	ACTTGGTGCTAATGTTGTTT+TGG	+	chr2.1:31355771-31355790	MS.gene62493:CDS	35.0%
	TGAAAACTCTCTCTGATGGA+GGG	-	chr2.1:31355381-31355400	None:intergenic	40.0%
	TGCCTCCTTCAAACCTAAAT+CGG	-	chr2.1:31355665-31355684	None:intergenic	40.0%
	TTAAAGTCATCAAGGAGGTG+CGG	+	chr2.1:31355632-31355651	MS.gene62493:CDS	40.0%
	TTGCAGGATCAAAATCAGCT+GGG	-	chr2.1:31355333-31355352	None:intergenic	40.0%
!	TTTAGGTTTGAAGGAGGCAA+AGG	+	chr2.1:31355666-31355685	MS.gene62493:CDS	40.0%
!!	TACCGATTTAGGTTTGAAGG+AGG	+	chr2.1:31355660-31355679	MS.gene62493:CDS	40.0%
!!	TGAGCTTGCTGTTGAGAATA+AGG	+	chr2.1:31355149-31355168	MS.gene62493:CDS	40.0%
!!	TTTCAGGCTTGTTGATGAAG+TGG	+	chr2.1:31355396-31355415	MS.gene62493:CDS	40.0%
!!!	TTCTGGTTTTACCTCCTCTT+GGG	-	chr2.1:31355563-31355582	None:intergenic	40.0%
!!!	TTTCTGGTTTTACCTCCTCT+TGG	-	chr2.1:31355564-31355583	None:intergenic	40.0%
	AGCCTGAAAACTCTCTCTGA+TGG	-	chr2.1:31355385-31355404	None:intergenic	45.0%
	CTGAAAACTCTCTCTGATGG+AGG	-	chr2.1:31355382-31355401	None:intergenic	45.0%
	GAAAGCAGGGTCTGCAAATT+TGG	+	chr2.1:31355507-31355526	MS.gene62493:CDS	45.0%
	GCGTATTAGGGCTTAATGCT+AGG	+	chr2.1:31355215-31355234	MS.gene62493:CDS	45.0%
	GTTGCAGGATCAAAATCAGC+TGG	-	chr2.1:31355334-31355353	None:intergenic	45.0%
	TCAAGCTGCAAGAAAAGAGG+AGG	+	chr2.1:31355267-31355286	MS.gene62493:CDS	45.0%
	TGGTCAAGCTGCAAGAAAAG+AGG	+	chr2.1:31355264-31355283	MS.gene62493:CDS	45.0%
!	CTCCATCAGAGAGAGTTTTC+AGG	+	chr2.1:31355380-31355399	MS.gene62493:CDS	45.0%
!	TAAAGTCATCAAGGAGGTGC+GGG	+	chr2.1:31355633-31355652	MS.gene62493:CDS	45.0%
	AGCTGCAAGAAAAGAGGAGG+AGG	+	chr2.1:31355270-31355289	MS.gene62493:CDS	50.0%
	GTCTGCAAATTTGGCCGGAA+TGG	+	chr2.1:31355516-31355535	MS.gene62493:CDS	50.0%
	TCTAACGCTAGCTGAAGGTG+CGG	+	chr2.1:31355423-31355442	MS.gene62493:CDS	50.0%
	TGGAGGGCCACGATTGTTAT+TGG	-	chr2.1:31355365-31355384	None:intergenic	50.0%
!	AAAGTCATCAAGGAGGTGCG+GGG	+	chr2.1:31355634-31355653	MS.gene62493:CDS	50.0%
!	CGTGGCTTTTGTTGCCATTC+CGG	-	chr2.1:31355533-31355552	None:intergenic	50.0%
!	CTAGCTGAAGGTGCGGAATT+GGG	+	chr2.1:31355430-31355449	MS.gene62493:CDS	50.0%
!	GGAGGGCCACGATTGTTATT+GGG	-	chr2.1:31355364-31355383	None:intergenic	50.0%
	AGAGGAGGAGGAAGACGTAG+AGG	+	chr2.1:31355282-31355301	MS.gene62493:CDS	55.0%
	GCAGGGTCTGCAAATTTGGC+CGG	+	chr2.1:31355511-31355530	MS.gene62493:CDS	55.0%
	GCGTCTCTAACGCTAGCTGA+AGG	+	chr2.1:31355418-31355437	MS.gene62493:CDS	55.0%
	GGAGGAAGACGTAGAGGAAG+TGG	+	chr2.1:31355288-31355307	MS.gene62493:CDS	55.0%
!	GCTAGCTGAAGGTGCGGAAT+TGG	+	chr2.1:31355429-31355448	MS.gene62493:CDS	55.0%
!!	GTGCGGGGCTTTACCGATTT+AGG	+	chr2.1:31355649-31355668	MS.gene62493:CDS	55.0%
	AGCCACGACTGCAGCCCAAG+AGG	+	chr2.1:31355546-31355565	MS.gene62493:CDS	65.0%
	CACGACTGCAGCCCAAGAGG+AGG	+	chr2.1:31355549-31355568	MS.gene62493:CDS	65.0%
	CTCCTCTTGGGCTGCAGTCG+TGG	-	chr2.1:31355551-31355570	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	31355148	31355798	31355148	ID=MS.gene62493
chr2.1	mRNA	31355148	31355798	31355148	ID=MS.gene62493.t1;Parent=MS.gene62493
chr2.1	exon	31355148	31355798	31355148	ID=MS.gene62493.t1.exon1;Parent=MS.gene62493.t1
chr2.1	CDS	31355148	31355798	31355148	ID=cds.MS.gene62493.t1;Parent=MS.gene62493.t1

Gene Sequence

>MS.gene62493

ATGAGCTTGCTGTTGAGAATAAGGCATCGTTTACACAATGGTTTGTATAGACAACCAGTTTATGAAAGCGTATTAGGGCTTAATGCTAGGAAATTAAATGTATTTTCGAGAAACTTTGGTCAAGCTGCAAGAAAAGAGGAGGAGGAAGACGTAGAGGAAGTGGAAATTGATCAAAGAAGTCTCCCAGCTGATTTTGATCCTGCAACATTTGATCCCAATAACAATCGTGGCCCTCCATCAGAGAGAGTTTTCAGGCTTGTTGATGAAGTGGCGTCTCTAACGCTAGCTGAAGGTGCGGAATTGGGTCTCATTTTGATGAAGAAAATGGGGATAAAAGAAATGCCCAATGTGGGATATATGAAAGCAGGGTCTGCAAATTTGGCCGGAATGGCAACAAAAGCCACGACTGCAGCCCAAGAGGAGGTAAAACCAGAAAAAACTGTATTTGAATTGAAACTGTTGTCGTATGAAGCAGCTTCCAAAATTAAAGTCATCAAGGAGGTGCGGGGCTTTACCGATTTAGGTTTGAAGGAGGCAAAGGATATTGTCGAAAAAACACCGTCTATTATAAAGAAAGGTGTTTCAAAGGAAGAAGGAGAACAGATAATAGAGAAAATGAAAGCACTTGGTGCTAATGTTGTTTTGGAGTGA

Protein sequence

>MS.gene62493.t1

MSLLLRIRHRLHNGLYRQPVYESVLGLNARKLNVFSRNFGQAARKEEEEDVEEVEIDQRSLPADFDPATFDPNNNRGPPSERVFRLVDEVASLTLAEGAELGLILMKKMGIKEMPNVGYMKAGSANLAGMATKATTAAQEEVKPEKTVFELKLLSYEAASKIKVIKEVRGFTDLGLKEAKDIVEKTPSIIKKGVSKEEGEQIIEKMKALGANVVLE