Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62846.t1 | XP_024638828.1 | 90.8 | 142 | 11 | 1 | 1 | 142 | 1 | 140 | 1.40E-60 | 242.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62846.t1 | G7JK81 | 92.7 | 110 | 8 | 0 | 1 | 110 | 1 | 110 | 1.1e-46 | 195.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054713 | MS.gene62846 | 0.831661 | 1.47E-55 | -1.69E-46 |
| MS.gene058042 | MS.gene62846 | 0.829389 | 5.29E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62846.t1 | MTR_4g102040 | 92.727 | 110 | 8 | 0 | 1 | 110 | 1 | 110 | 5.61e-69 | 206 |
| MS.gene62846.t1 | MTR_5g011750 | 62.162 | 74 | 23 | 1 | 41 | 109 | 5 | 78 | 2.36e-23 | 88.2 |
| MS.gene62846.t1 | MTR_4g055420 | 53.846 | 52 | 24 | 0 | 57 | 108 | 69 | 120 | 7.95e-11 | 57.8 |
| MS.gene62846.t1 | MTR_2g084805 | 60.417 | 48 | 19 | 0 | 57 | 104 | 57 | 104 | 8.48e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCAGTGTTCTTCAAAAT+TGG | 0.195240 | 8.4:-22927707 | None:intergenic |
| GTTTCTCTGGCTGTGAATTA+TGG | 0.269497 | 8.4:-22927475 | None:intergenic |
| GACAACATCAACATCTGAAT+TGG | 0.306262 | 8.4:-22927620 | None:intergenic |
| AAACTAATGACACTGTTCTT+AGG | 0.355610 | 8.4:+22927552 | MS.gene62846:CDS |
| CACAGTTGAAGATTTGATAA+AGG | 0.384204 | 8.4:+22927649 | MS.gene62846:CDS |
| AGACGAGAAGTTAGTGGGCT+TGG | 0.389634 | 8.4:+22928063 | MS.gene62846:CDS |
| GCTGTGAATTATGGAGTTTA+CGG | 0.413396 | 8.4:-22927466 | None:intergenic |
| AGAATTCAACGGGGTCTTAC+CGG | 0.420392 | 8.4:-22927441 | None:intergenic |
| GTTTACGGCAAGAATTCAAC+GGG | 0.423723 | 8.4:-22927451 | None:intergenic |
| ACATTGCCAGAGGGTTTCTC+TGG | 0.447363 | 8.4:-22927488 | None:intergenic |
| ACAACATCAACATCTGAATT+GGG | 0.506453 | 8.4:-22927619 | None:intergenic |
| CTGCATGGTGCATGAAGAAA+CGG | 0.506540 | 8.4:-22928125 | None:intergenic |
| AAGCATGACTTCTCCGCCAC+AGG | 0.512058 | 8.4:+22927400 | None:intergenic |
| GAAGCGAGACGAGAAGTTAG+TGG | 0.521335 | 8.4:+22928057 | MS.gene62846:CDS |
| CTTCTCCGCCACAGGCTCAT+CGG | 0.524598 | 8.4:+22927408 | MS.gene62846:CDS |
| GTGACAATCGAAAACAGCTT+GGG | 0.531357 | 8.4:+22927596 | MS.gene62846:CDS |
| AGTTAGTGGGCTTGGAGTCA+AGG | 0.535890 | 8.4:+22928071 | MS.gene62846:CDS |
| GGTGACAATCGAAAACAGCT+TGG | 0.547560 | 8.4:+22927595 | MS.gene62846:CDS |
| TGCCACATTCTCATTCTGCA+TGG | 0.554134 | 8.4:-22928140 | None:intergenic |
| GTTTGCATCTTCAGCGTCAG+AGG | 0.563732 | 8.4:-22927533 | None:intergenic |
| AAGCGAGACGAGAAGTTAGT+GGG | 0.573862 | 8.4:+22928058 | MS.gene62846:CDS |
| AGATTTGATAAAGGCAGCGT+TGG | 0.574906 | 8.4:+22927658 | MS.gene62846:CDS |
| CCTCTGGCAATGTTGATCGT+CGG | 0.591836 | 8.4:+22927498 | MS.gene62846:CDS |
| TCACAGCCAGAGAAACCCTC+TGG | 0.594528 | 8.4:+22927482 | MS.gene62846:CDS |
| TTTCTCCGATGAGCCTGTGG+CGG | 0.602364 | 8.4:-22927413 | None:intergenic |
| GCCATCAAAACTGCTACTGA+AGG | 0.608756 | 8.4:+22927574 | MS.gene62846:CDS |
| TGCATGGTGCATGAAGAAAC+GGG | 0.610698 | 8.4:-22928124 | None:intergenic |
| AGTTTACGGCAAGAATTCAA+CGG | 0.621562 | 8.4:-22927452 | None:intergenic |
| ATTCAACGGGGTCTTACCGG+AGG | 0.655189 | 8.4:-22927438 | None:intergenic |
| CGACGATCAACATTGCCAGA+GGG | 0.655617 | 8.4:-22927497 | None:intergenic |
| TCAGATGTTGATGTTGTCTG+AGG | 0.666940 | 8.4:+22927625 | MS.gene62846:CDS |
| TTTATGAGAAGGATAAGAGG+AGG | 0.687109 | 8.4:+22927684 | MS.gene62846:CDS |
| CCGACGATCAACATTGCCAG+AGG | 0.706693 | 8.4:-22927498 | None:intergenic |
| ATGGTGCATGAAGAAACGGG+TGG | 0.830690 | 8.4:-22928121 | None:intergenic |
| TTTACGGCAAGAATTCAACG+GGG | 0.837986 | 8.4:-22927450 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAAAATGATTGTTTTTTTT+AGG | - | chr8.4:22928023-22928042 | None:intergenic | 15.0% |
| !!! | AAAAAACAATCATTTTGAGT+TGG | + | chr8.4:22928025-22928044 | MS.gene62846:intron | 20.0% |
| ! | TTAGTAAGAAGTCATTACTT+TGG | - | chr8.4:22927769-22927788 | None:intergenic | 25.0% |
| !! | ATTACTTTGGAAAGTGAATT+GGG | - | chr8.4:22927756-22927775 | None:intergenic | 25.0% |
| !!! | AATGTTCTGTTTTTCATTCA+TGG | + | chr8.4:22927824-22927843 | MS.gene62846:intron | 25.0% |
| AAACTAATGACACTGTTCTT+AGG | + | chr8.4:22927552-22927571 | MS.gene62846:CDS | 30.0% | |
| ACAACATCAACATCTGAATT+GGG | - | chr8.4:22927622-22927641 | None:intergenic | 30.0% | |
| AGCTCAAAACATAGAATTAG+GGG | - | chr8.4:22927799-22927818 | None:intergenic | 30.0% | |
| CACAGTTGAAGATTTGATAA+AGG | + | chr8.4:22927649-22927668 | MS.gene62846:CDS | 30.0% | |
| CAGCTCAAAACATAGAATTA+GGG | - | chr8.4:22927800-22927819 | None:intergenic | 30.0% | |
| TAATGAAGATCATAACACCT+TGG | - | chr8.4:22927731-22927750 | None:intergenic | 30.0% | |
| TCAGCTCAAAACATAGAATT+AGG | - | chr8.4:22927801-22927820 | None:intergenic | 30.0% | |
| ! | CATTACTTTGGAAAGTGAAT+TGG | - | chr8.4:22927757-22927776 | None:intergenic | 30.0% |
| ! | TGTTTTATGAGAAGGATAAG+AGG | + | chr8.4:22927681-22927700 | MS.gene62846:CDS | 30.0% |
| AGTTTACGGCAAGAATTCAA+CGG | - | chr8.4:22927455-22927474 | None:intergenic | 35.0% | |
| GACAACATCAACATCTGAAT+TGG | - | chr8.4:22927623-22927642 | None:intergenic | 35.0% | |
| GCTGTGAATTATGGAGTTTA+CGG | - | chr8.4:22927469-22927488 | None:intergenic | 35.0% | |
| GGATCAGTGTTCTTCAAAAT+TGG | - | chr8.4:22927710-22927729 | None:intergenic | 35.0% | |
| TTTATGAGAAGGATAAGAGG+AGG | + | chr8.4:22927684-22927703 | MS.gene62846:CDS | 35.0% | |
| !! | TCTGTTTTTCATTCATGGAC+TGG | + | chr8.4:22927829-22927848 | MS.gene62846:intron | 35.0% |
| !! | TTTTGAAGAACACTGATCCA+AGG | + | chr8.4:22927711-22927730 | MS.gene62846:CDS | 35.0% |
| AGATTTGATAAAGGCAGCGT+TGG | + | chr8.4:22927658-22927677 | MS.gene62846:CDS | 40.0% | |
| GTGACAATCGAAAACAGCTT+GGG | + | chr8.4:22927596-22927615 | MS.gene62846:CDS | 40.0% | |
| GTTTACGGCAAGAATTCAAC+GGG | - | chr8.4:22927454-22927473 | None:intergenic | 40.0% | |
| GTTTCTCTGGCTGTGAATTA+TGG | - | chr8.4:22927478-22927497 | None:intergenic | 40.0% | |
| TCAGATGTTGATGTTGTCTG+AGG | + | chr8.4:22927625-22927644 | MS.gene62846:CDS | 40.0% | |
| TTTACGGCAAGAATTCAACG+GGG | - | chr8.4:22927453-22927472 | None:intergenic | 40.0% | |
| !! | ACCTTCAGTAGCAGTTTTGA+TGG | - | chr8.4:22927578-22927597 | None:intergenic | 40.0% |
| !! | CATTCATGGACTGGCTAATT+TGG | + | chr8.4:22927838-22927857 | MS.gene62846:intron | 40.0% |
| !!! | AGCGTTGGTGTTTTATGAGA+AGG | + | chr8.4:22927673-22927692 | MS.gene62846:CDS | 40.0% |
| !!! | CAATCATTTTGAGTTGGTGC+AGG | + | chr8.4:22928031-22928050 | MS.gene62846:intron | 40.0% |
| AAGCGAGACGAGAAGTTAGT+GGG | + | chr8.4:22928058-22928077 | MS.gene62846:CDS | 45.0% | |
| AGAATTCAACGGGGTCTTAC+CGG | - | chr8.4:22927444-22927463 | None:intergenic | 45.0% | |
| CTGCATGGTGCATGAAGAAA+CGG | - | chr8.4:22928128-22928147 | None:intergenic | 45.0% | |
| GCCATCAAAACTGCTACTGA+AGG | + | chr8.4:22927574-22927593 | MS.gene62846:CDS | 45.0% | |
| GGTGACAATCGAAAACAGCT+TGG | + | chr8.4:22927595-22927614 | MS.gene62846:CDS | 45.0% | |
| TGCATGGTGCATGAAGAAAC+GGG | - | chr8.4:22928127-22928146 | None:intergenic | 45.0% | |
| ACATTGCCAGAGGGTTTCTC+TGG | - | chr8.4:22927491-22927510 | None:intergenic | 50.0% | |
| ATGGTGCATGAAGAAACGGG+TGG | - | chr8.4:22928124-22928143 | None:intergenic | 50.0% | |
| CCTCTGGCAATGTTGATCGT+CGG | + | chr8.4:22927498-22927517 | MS.gene62846:CDS | 50.0% | |
| CGACGATCAACATTGCCAGA+GGG | - | chr8.4:22927500-22927519 | None:intergenic | 50.0% | |
| GAAGCGAGACGAGAAGTTAG+TGG | + | chr8.4:22928057-22928076 | MS.gene62846:CDS | 50.0% | |
| GCTCATCGGAGAAAAACCTC+CGG | + | chr8.4:22927422-22927441 | MS.gene62846:CDS | 50.0% | |
| GTTTGCATCTTCAGCGTCAG+AGG | - | chr8.4:22927536-22927555 | None:intergenic | 50.0% | |
| ! | GTTTTTCTCCGATGAGCCTG+TGG | - | chr8.4:22927419-22927438 | None:intergenic | 50.0% |
| !! | AGACGAGAAGTTAGTGGGCT+TGG | + | chr8.4:22928063-22928082 | MS.gene62846:CDS | 50.0% |
| !! | AGTTAGTGGGCTTGGAGTCA+AGG | + | chr8.4:22928071-22928090 | MS.gene62846:CDS | 50.0% |
| ATTCAACGGGGTCTTACCGG+AGG | - | chr8.4:22927441-22927460 | None:intergenic | 55.0% | |
| CCGACGATCAACATTGCCAG+AGG | - | chr8.4:22927501-22927520 | None:intergenic | 55.0% | |
| TCACAGCCAGAGAAACCCTC+TGG | + | chr8.4:22927482-22927501 | MS.gene62846:CDS | 55.0% | |
| TTTCTCCGATGAGCCTGTGG+CGG | - | chr8.4:22927416-22927435 | None:intergenic | 55.0% | |
| CTTCTCCGCCACAGGCTCAT+CGG | + | chr8.4:22927408-22927427 | MS.gene62846:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 22927404 | 22928152 | 22927404 | ID=MS.gene62846 |
| chr8.4 | mRNA | 22927404 | 22928152 | 22927404 | ID=MS.gene62846.t1;Parent=MS.gene62846 |
| chr8.4 | exon | 22927404 | 22927732 | 22927404 | ID=MS.gene62846.t1.exon1;Parent=MS.gene62846.t1 |
| chr8.4 | CDS | 22927404 | 22927732 | 22927404 | ID=cds.MS.gene62846.t1;Parent=MS.gene62846.t1 |
| chr8.4 | exon | 22928053 | 22928152 | 22928053 | ID=MS.gene62846.t1.exon2;Parent=MS.gene62846.t1 |
| chr8.4 | CDS | 22928053 | 22928152 | 22928053 | ID=cds.MS.gene62846.t1;Parent=MS.gene62846.t1 |
| Gene Sequence |
| Protein sequence |
