Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63067.t1 | XP_003600750.2 | 72.1 | 122 | 34 | 0 | 1 | 122 | 19 | 140 | 1.60E-44 | 188.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63067.t1 | P62926 | 45.9 | 111 | 46 | 6 | 9 | 117 | 27 | 125 | 2.0e-16 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63067.t1 | G7J9G6 | 72.1 | 122 | 34 | 0 | 1 | 122 | 19 | 140 | 1.1e-44 | 188.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04925 | MS.gene63067 | 0.914589 | 1.61E-84 | -1.69E-46 |
| MS.gene04926 | MS.gene63067 | 0.862901 | 3.65E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAATTCTAATCTCAAATA+TGG | 0.216722 | 3.4:+67877324 | MS.gene63067:CDS |
| CCATATTTGAGATTAGAATT+TGG | 0.241765 | 3.4:-67877324 | None:intergenic |
| TGTACGATCGGTTATTGTTA+TGG | 0.354008 | 3.4:+67877177 | MS.gene63067:CDS |
| TTTGCTTCTACTTTCTTCAT+TGG | 0.374147 | 3.4:-67877084 | None:intergenic |
| CTTGTAAGCGTCTTGTGCTT+CGG | 0.409202 | 3.4:-67877367 | None:intergenic |
| GAAAATCGCAATGTGGTAAG+TGG | 0.449479 | 3.4:+67877207 | MS.gene63067:CDS |
| TTGACATATCAATATTAAGC+AGG | 0.480895 | 3.4:-67877438 | None:intergenic |
| TTTCTTGAATTTATAGCTAG+AGG | 0.515321 | 3.4:-67877394 | None:intergenic |
| AGGAACATCCTAAGTTATGT+TGG | 0.542665 | 3.4:+67877250 | MS.gene63067:CDS |
| TCATGAGACCAACATAACTT+AGG | 0.547157 | 3.4:-67877258 | None:intergenic |
| AAAGTAGAAGCAAATTGTGA+TGG | 0.553230 | 3.4:+67877093 | MS.gene63067:CDS |
| AAACTCGTTGGTTGTACGAT+CGG | 0.575852 | 3.4:+67877165 | MS.gene63067:CDS |
| AGCACAAGACGCTTACAAGA+TGG | 0.577959 | 3.4:+67877371 | MS.gene63067:CDS |
| AAAATCGCAATGTGGTAAGT+GGG | 0.583821 | 3.4:+67877208 | MS.gene63067:CDS |
| GGTTGTAAATGCAAACTCGT+TGG | 0.593162 | 3.4:+67877153 | MS.gene63067:CDS |
| GTCTTGTGCTTCGGAGACAG+AGG | 0.642879 | 3.4:-67877358 | None:intergenic |
| TTTATGCTAGACTGTGATAG+CGG | 0.659883 | 3.4:+67877132 | MS.gene63067:CDS |
| TACCTGCGAAAATCGCAATG+TGG | 0.731640 | 3.4:+67877200 | MS.gene63067:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CCAAATTCTAATCTCAAATA+TGG | + | chr3.4:67877324-67877343 | MS.gene63067:CDS | 25.0% |
| ! | TTTCTTGAATTTATAGCTAG+AGG | - | chr3.4:67877397-67877416 | None:intergenic | 25.0% |
| !! | CCATATTTGAGATTAGAATT+TGG | - | chr3.4:67877327-67877346 | None:intergenic | 25.0% |
| AAAGTAGAAGCAAATTGTGA+TGG | + | chr3.4:67877093-67877112 | MS.gene63067:CDS | 30.0% | |
| CATGATGAATGCACAAAAAA+GGG | + | chr3.4:67877276-67877295 | MS.gene63067:CDS | 30.0% | |
| TCATGATGAATGCACAAAAA+AGG | + | chr3.4:67877275-67877294 | MS.gene63067:CDS | 30.0% | |
| TTTGCTTCTACTTTCTTCAT+TGG | - | chr3.4:67877087-67877106 | None:intergenic | 30.0% | |
| AAAATCGCAATGTGGTAAGT+GGG | + | chr3.4:67877208-67877227 | MS.gene63067:CDS | 35.0% | |
| AATGCACAAAAAAGGGTAGT+GGG | + | chr3.4:67877283-67877302 | MS.gene63067:CDS | 35.0% | |
| AGGAACATCCTAAGTTATGT+TGG | + | chr3.4:67877250-67877269 | MS.gene63067:CDS | 35.0% | |
| TCATGAGACCAACATAACTT+AGG | - | chr3.4:67877261-67877280 | None:intergenic | 35.0% | |
| TGTACGATCGGTTATTGTTA+TGG | + | chr3.4:67877177-67877196 | MS.gene63067:CDS | 35.0% | |
| TTTATGCTAGACTGTGATAG+CGG | + | chr3.4:67877132-67877151 | MS.gene63067:CDS | 35.0% | |
| ! | GAATTTTCAGAGGAAGGTTA+AGG | + | chr3.4:67877230-67877249 | MS.gene63067:CDS | 35.0% |
| GAAAATCGCAATGTGGTAAG+TGG | + | chr3.4:67877207-67877226 | MS.gene63067:CDS | 40.0% | |
| GAATGCACAAAAAAGGGTAG+TGG | + | chr3.4:67877282-67877301 | MS.gene63067:CDS | 40.0% | |
| GGTTGTAAATGCAAACTCGT+TGG | + | chr3.4:67877153-67877172 | MS.gene63067:CDS | 40.0% | |
| ! | AAACTCGTTGGTTGTACGAT+CGG | + | chr3.4:67877165-67877184 | MS.gene63067:CDS | 40.0% |
| ! | AAGTGGGAATTTTCAGAGGA+AGG | + | chr3.4:67877224-67877243 | MS.gene63067:CDS | 40.0% |
| ! | TGGTAAGTGGGAATTTTCAG+AGG | + | chr3.4:67877220-67877239 | MS.gene63067:CDS | 40.0% |
| AGCACAAGACGCTTACAAGA+TGG | + | chr3.4:67877371-67877390 | MS.gene63067:CDS | 45.0% | |
| CTTGTAAGCGTCTTGTGCTT+CGG | - | chr3.4:67877370-67877389 | None:intergenic | 45.0% | |
| TACCTGCGAAAATCGCAATG+TGG | + | chr3.4:67877200-67877219 | MS.gene63067:CDS | 45.0% | |
| ! | TACCACATTGCGATTTTCGC+AGG | - | chr3.4:67877205-67877224 | None:intergenic | 45.0% |
| GTCTTGTGCTTCGGAGACAG+AGG | - | chr3.4:67877361-67877380 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 67877076 | 67877446 | 67877076 | ID=MS.gene63067 |
| chr3.4 | mRNA | 67877076 | 67877446 | 67877076 | ID=MS.gene63067.t1;Parent=MS.gene63067 |
| chr3.4 | exon | 67877076 | 67877446 | 67877076 | ID=MS.gene63067.t1.exon1;Parent=MS.gene63067.t1 |
| chr3.4 | CDS | 67877076 | 67877446 | 67877076 | ID=cds.MS.gene63067.t1;Parent=MS.gene63067.t1 |
| Gene Sequence |
| Protein sequence |
