Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04925.t1 | XP_003600750.2 | 75.7 | 140 | 34 | 0 | 1 | 140 | 1 | 140 | 6.20E-53 | 216.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04925.t1 | P62926 | 43.8 | 137 | 63 | 6 | 1 | 135 | 1 | 125 | 4.2e-18 | 92.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04925.t1 | G7J9G6 | 75.7 | 140 | 34 | 0 | 1 | 140 | 1 | 140 | 4.4e-53 | 216.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04925 | MS.gene04926 | 0.846721 | 1.82E-59 | -1.69E-46 |
| MS.gene04925 | MS.gene054277 | 0.810184 | 1.31E-50 | -1.69E-46 |
| MS.gene04925 | MS.gene060087 | 0.828965 | 6.70E-55 | -1.69E-46 |
| MS.gene04925 | MS.gene061038 | 0.832947 | 7.07E-56 | -1.69E-46 |
| MS.gene04925 | MS.gene064807 | 0.804399 | 2.20E-49 | -1.69E-46 |
| MS.gene04925 | MS.gene066602 | 0.820755 | 5.80E-53 | -1.69E-46 |
| MS.gene04925 | MS.gene07803 | 0.806747 | 7.08E-50 | -1.69E-46 |
| MS.gene04925 | MS.gene31542 | 0.822644 | 2.12E-53 | -1.69E-46 |
| MS.gene04925 | MS.gene35106 | 0.806887 | 6.61E-50 | -1.69E-46 |
| MS.gene04925 | MS.gene35741 | 0.836366 | 9.76E-57 | -1.69E-46 |
| MS.gene04925 | MS.gene42005 | 0.820315 | 7.32E-53 | -1.69E-46 |
| MS.gene04925 | MS.gene44958 | 0.817454 | 3.27E-52 | -1.69E-46 |
| MS.gene04925 | MS.gene48885 | 0.818161 | 2.27E-52 | -1.69E-46 |
| MS.gene04925 | MS.gene50279 | 0.824065 | 9.88E-54 | -1.69E-46 |
| MS.gene04925 | MS.gene51595 | 0.83714 | 6.19E-57 | -1.69E-46 |
| MS.gene04925 | MS.gene55239 | 0.805035 | 1.62E-49 | -1.69E-46 |
| MS.gene04925 | MS.gene63067 | 0.914589 | 1.61E-84 | -1.69E-46 |
| MS.gene04925 | MS.gene75189 | 0.814634 | 1.39E-51 | -1.69E-46 |
| MS.gene04925 | MS.gene77325 | 0.814982 | 1.17E-51 | -1.69E-46 |
| MS.gene04925 | MS.gene77595 | 0.801274 | 9.73E-49 | -1.69E-46 |
| MS.gene04925 | MS.gene80293 | 0.817159 | 3.81E-52 | -1.69E-46 |
| MS.gene04925 | MS.gene80468 | 0.809196 | 2.13E-50 | -1.69E-46 |
| MS.gene04925 | MS.gene81503 | 0.800451 | 1.43E-48 | -1.69E-46 |
| MS.gene04925 | MS.gene81504 | 0.803551 | 3.30E-49 | -1.69E-46 |
| MS.gene04925 | MS.gene91226 | 0.80183 | 7.48E-49 | -1.69E-46 |
| MS.gene04925 | MS.gene95747 | 0.807037 | 6.15E-50 | -1.69E-46 |
| MS.gene04925 | MS.gene96808 | 0.8083 | 3.31E-50 | -1.69E-46 |
| MS.gene04925 | MS.gene97561 | 0.808397 | 3.16E-50 | -1.69E-46 |
| MS.gene04925 | MS.gene99062 | 0.822695 | 2.06E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04925.t1 | MTR_3g067430 | 75.714 | 140 | 34 | 0 | 1 | 140 | 1 | 140 | 1.61e-71 | 211 |
| MS.gene04925.t1 | MTR_3g067445 | 71.795 | 117 | 33 | 0 | 1 | 117 | 1 | 117 | 1.88e-55 | 169 |
| MS.gene04925.t1 | MTR_3g067540 | 58.865 | 141 | 56 | 2 | 1 | 140 | 1 | 140 | 2.23e-50 | 157 |
| MS.gene04925.t1 | MTR_3g067555 | 58.865 | 141 | 56 | 2 | 1 | 140 | 1 | 140 | 2.23e-50 | 157 |
| MS.gene04925.t1 | MTR_3g067535 | 58.571 | 140 | 54 | 2 | 1 | 140 | 1 | 136 | 8.28e-47 | 148 |
| MS.gene04925.t1 | MTR_3g067580 | 52.083 | 144 | 57 | 3 | 1 | 140 | 9 | 144 | 1.05e-43 | 140 |
| MS.gene04925.t1 | MTR_3g067280 | 57.664 | 137 | 50 | 5 | 1 | 136 | 1 | 130 | 2.32e-42 | 137 |
| MS.gene04925.t1 | MTR_6g036620 | 52.817 | 142 | 57 | 4 | 1 | 140 | 1 | 134 | 1.03e-41 | 135 |
| MS.gene04925.t1 | MTR_3g067555 | 56.098 | 123 | 54 | 0 | 18 | 140 | 5 | 127 | 1.63e-40 | 132 |
| MS.gene04925.t1 | MTR_3g067510 | 51.429 | 140 | 58 | 2 | 1 | 140 | 1 | 130 | 2.45e-40 | 132 |
| MS.gene04925.t1 | MTR_3g067437 | 52.593 | 135 | 56 | 4 | 1 | 133 | 1 | 129 | 2.51e-40 | 131 |
| MS.gene04925.t1 | MTR_3g067270 | 54.745 | 137 | 54 | 4 | 1 | 136 | 1 | 130 | 6.36e-40 | 130 |
| MS.gene04925.t1 | MTR_3g467750 | 54.745 | 137 | 54 | 4 | 1 | 136 | 1 | 130 | 6.36e-40 | 130 |
| MS.gene04925.t1 | MTR_3g067830 | 68.932 | 103 | 29 | 3 | 1 | 102 | 1 | 101 | 8.65e-39 | 127 |
| MS.gene04925.t1 | MTR_3g067510 | 50.345 | 145 | 57 | 3 | 1 | 140 | 1 | 135 | 2.14e-38 | 127 |
| MS.gene04925.t1 | MTR_3g067550 | 55.462 | 119 | 53 | 0 | 22 | 140 | 1 | 119 | 8.99e-38 | 125 |
| MS.gene04925.t1 | MTR_3g067555 | 55.462 | 119 | 53 | 0 | 22 | 140 | 1 | 119 | 8.99e-38 | 125 |
| MS.gene04925.t1 | MTR_3g467760 | 51.471 | 136 | 36 | 3 | 1 | 136 | 1 | 106 | 4.73e-37 | 122 |
| MS.gene04925.t1 | MTR_3g463570 | 49.286 | 140 | 63 | 4 | 1 | 138 | 1 | 134 | 1.05e-34 | 117 |
| MS.gene04925.t1 | MTR_7g056803 | 46.154 | 143 | 69 | 3 | 1 | 140 | 1 | 138 | 5.54e-33 | 113 |
| MS.gene04925.t1 | MTR_7g056817 | 46.528 | 144 | 68 | 5 | 1 | 140 | 1 | 139 | 1.41e-32 | 112 |
| MS.gene04925.t1 | MTR_3g067500 | 54.167 | 120 | 48 | 3 | 22 | 140 | 1 | 114 | 9.44e-31 | 107 |
| MS.gene04925.t1 | MTR_8g022430 | 43.165 | 139 | 70 | 4 | 1 | 136 | 1 | 133 | 4.52e-30 | 106 |
| MS.gene04925.t1 | MTR_6g017150 | 44.681 | 141 | 65 | 7 | 1 | 136 | 1 | 133 | 1.33e-29 | 104 |
| MS.gene04925.t1 | MTR_6g047880 | 41.259 | 143 | 67 | 4 | 1 | 136 | 1 | 133 | 2.64e-29 | 103 |
| MS.gene04925.t1 | MTR_3g067570 | 53.535 | 99 | 39 | 3 | 22 | 120 | 1 | 92 | 1.66e-28 | 100 |
| MS.gene04925.t1 | MTR_8g022420 | 42.647 | 136 | 68 | 5 | 1 | 133 | 1 | 129 | 4.08e-28 | 101 |
| MS.gene04925.t1 | MTR_8g022400 | 43.571 | 140 | 67 | 7 | 1 | 136 | 1 | 132 | 2.04e-27 | 99.4 |
| MS.gene04925.t1 | MTR_1g019650 | 42.520 | 127 | 55 | 3 | 1 | 126 | 1 | 110 | 3.26e-27 | 97.8 |
| MS.gene04925.t1 | MTR_7g044920 | 42.581 | 155 | 65 | 5 | 1 | 138 | 1 | 148 | 4.76e-27 | 99.0 |
| MS.gene04925.t1 | MTR_6g047900 | 41.135 | 141 | 67 | 4 | 1 | 136 | 1 | 130 | 6.60e-27 | 97.8 |
| MS.gene04925.t1 | MTR_3g436100 | 38.961 | 154 | 74 | 6 | 1 | 138 | 1 | 150 | 7.82e-27 | 98.2 |
| MS.gene04925.t1 | MTR_6g017170 | 42.424 | 132 | 67 | 4 | 1 | 129 | 1 | 126 | 2.49e-26 | 96.3 |
| MS.gene04925.t1 | MTR_3g463570 | 44.094 | 127 | 64 | 3 | 13 | 138 | 34 | 154 | 8.92e-26 | 95.5 |
| MS.gene04925.t1 | MTR_7g044980 | 43.548 | 124 | 59 | 4 | 1 | 123 | 1 | 114 | 5.24e-24 | 90.9 |
| MS.gene04925.t1 | MTR_8g461020 | 38.843 | 121 | 64 | 2 | 3 | 121 | 4 | 116 | 2.98e-23 | 88.6 |
| MS.gene04925.t1 | MTR_4g026590 | 41.781 | 146 | 68 | 5 | 1 | 136 | 1 | 139 | 2.99e-23 | 89.0 |
| MS.gene04925.t1 | MTR_7g029540 | 36.719 | 128 | 70 | 5 | 1 | 124 | 1 | 121 | 3.63e-17 | 72.8 |
| MS.gene04925.t1 | MTR_6g082060 | 38.060 | 134 | 70 | 6 | 1 | 127 | 1 | 128 | 7.92e-17 | 72.4 |
| MS.gene04925.t1 | MTR_8g056800 | 36.458 | 96 | 40 | 2 | 64 | 138 | 2 | 97 | 3.86e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAATTCTAATCTCAAATA+TGG | 0.216722 | 3.4:+67865937 | MS.gene04925:CDS |
| CCATATTTGAGATTAGAATT+TGG | 0.241765 | 3.4:-67865937 | None:intergenic |
| TGTACGATCGGTTATTGTTA+TGG | 0.354008 | 3.4:+67865790 | MS.gene04925:CDS |
| GTCTTCTTGCTTGCTGCATT+CGG | 0.367498 | 3.4:+67865501 | MS.gene04925:CDS |
| TTTGCTTCTACTTTCTTCAT+TGG | 0.374147 | 3.4:-67865697 | None:intergenic |
| CTTGTAAGCGTCTTGTGCTT+CGG | 0.409202 | 3.4:-67865980 | None:intergenic |
| GAAAATCGCAATGTGGTAAG+TGG | 0.449479 | 3.4:+67865820 | MS.gene04925:CDS |
| TTGACATATCAATATTAAGC+AGG | 0.480895 | 3.4:-67866051 | None:intergenic |
| TTTCTTGAATTTATAGCTAG+AGG | 0.515321 | 3.4:-67866007 | None:intergenic |
| AGGAACATCCTAAGTTATGT+TGG | 0.542665 | 3.4:+67865863 | MS.gene04925:CDS |
| TCATGAGACCAACATAACTT+AGG | 0.547157 | 3.4:-67865871 | None:intergenic |
| AAAGTAGAAGCAAATTGTGA+TGG | 0.553230 | 3.4:+67865706 | MS.gene04925:CDS |
| AAACTCGTTGGTTGTACGAT+CGG | 0.575852 | 3.4:+67865778 | MS.gene04925:CDS |
| AGCACAAGACGCTTACAAGA+TGG | 0.577959 | 3.4:+67865984 | MS.gene04925:CDS |
| AAAATCGCAATGTGGTAAGT+GGG | 0.583821 | 3.4:+67865821 | MS.gene04925:CDS |
| GGTTGTAAATGCAAACTCGT+TGG | 0.593162 | 3.4:+67865766 | MS.gene04925:CDS |
| GTCTTGTGCTTCGGAGACAG+AGG | 0.642879 | 3.4:-67865971 | None:intergenic |
| TTTATGCTAGACTGTGATAG+CGG | 0.659883 | 3.4:+67865745 | MS.gene04925:CDS |
| TACCTGCGAAAATCGCAATG+TGG | 0.731640 | 3.4:+67865813 | MS.gene04925:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTATTATTATATTAA+TGG | + | chr3.4:67865622-67865641 | MS.gene04925:intron | 0.0% |
| ! | AATGCATGAATGGTATATAT+TGG | - | chr3.4:67865551-67865570 | None:intergenic | 25.0% |
| ! | CATAAATAGAAATGCATGAA+TGG | - | chr3.4:67865561-67865580 | None:intergenic | 25.0% |
| ! | CCAAATTCTAATCTCAAATA+TGG | + | chr3.4:67865937-67865956 | MS.gene04925:CDS | 25.0% |
| ! | TTTCTTGAATTTATAGCTAG+AGG | - | chr3.4:67866010-67866029 | None:intergenic | 25.0% |
| !! | CCATATTTGAGATTAGAATT+TGG | - | chr3.4:67865940-67865959 | None:intergenic | 25.0% |
| AAAGTAGAAGCAAATTGTGA+TGG | + | chr3.4:67865706-67865725 | MS.gene04925:CDS | 30.0% | |
| CATGATGAATGCACAAAAAA+GGG | + | chr3.4:67865889-67865908 | MS.gene04925:CDS | 30.0% | |
| TCATGATGAATGCACAAAAA+AGG | + | chr3.4:67865888-67865907 | MS.gene04925:CDS | 30.0% | |
| TTTGCTTCTACTTTCTTCAT+TGG | - | chr3.4:67865700-67865719 | None:intergenic | 30.0% | |
| AAAATCGCAATGTGGTAAGT+GGG | + | chr3.4:67865821-67865840 | MS.gene04925:CDS | 35.0% | |
| AATGCACAAAAAAGGGTAGT+GGG | + | chr3.4:67865896-67865915 | MS.gene04925:CDS | 35.0% | |
| AGGAACATCCTAAGTTATGT+TGG | + | chr3.4:67865863-67865882 | MS.gene04925:CDS | 35.0% | |
| TCATGAGACCAACATAACTT+AGG | - | chr3.4:67865874-67865893 | None:intergenic | 35.0% | |
| TGTACGATCGGTTATTGTTA+TGG | + | chr3.4:67865790-67865809 | MS.gene04925:CDS | 35.0% | |
| TTTATGCTAGACTGTGATAG+CGG | + | chr3.4:67865745-67865764 | MS.gene04925:CDS | 35.0% | |
| ! | GAATTTTCAGAGGAAGGTTA+AGG | + | chr3.4:67865843-67865862 | MS.gene04925:CDS | 35.0% |
| GAAAATCGCAATGTGGTAAG+TGG | + | chr3.4:67865820-67865839 | MS.gene04925:CDS | 40.0% | |
| GAATGCACAAAAAAGGGTAG+TGG | + | chr3.4:67865895-67865914 | MS.gene04925:CDS | 40.0% | |
| GGTTGTAAATGCAAACTCGT+TGG | + | chr3.4:67865766-67865785 | MS.gene04925:CDS | 40.0% | |
| ! | AAACTCGTTGGTTGTACGAT+CGG | + | chr3.4:67865778-67865797 | MS.gene04925:CDS | 40.0% |
| ! | AAGTGGGAATTTTCAGAGGA+AGG | + | chr3.4:67865837-67865856 | MS.gene04925:CDS | 40.0% |
| ! | TGGTAAGTGGGAATTTTCAG+AGG | + | chr3.4:67865833-67865852 | MS.gene04925:CDS | 40.0% |
| AGCACAAGACGCTTACAAGA+TGG | + | chr3.4:67865984-67866003 | MS.gene04925:CDS | 45.0% | |
| CTTGTAAGCGTCTTGTGCTT+CGG | - | chr3.4:67865983-67866002 | None:intergenic | 45.0% | |
| TACCTGCGAAAATCGCAATG+TGG | + | chr3.4:67865813-67865832 | MS.gene04925:CDS | 45.0% | |
| ! | GTCTTCTTGCTTGCTGCATT+CGG | + | chr3.4:67865501-67865520 | MS.gene04925:CDS | 45.0% |
| ! | TACCACATTGCGATTTTCGC+AGG | - | chr3.4:67865818-67865837 | None:intergenic | 45.0% |
| GTCTTGTGCTTCGGAGACAG+AGG | - | chr3.4:67865974-67865993 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 67865471 | 67866059 | 67865471 | ID=MS.gene04925 |
| chr3.4 | mRNA | 67865471 | 67866059 | 67865471 | ID=MS.gene04925.t1;Parent=MS.gene04925 |
| chr3.4 | exon | 67865471 | 67865522 | 67865471 | ID=MS.gene04925.t1.exon1;Parent=MS.gene04925.t1 |
| chr3.4 | CDS | 67865471 | 67865522 | 67865471 | ID=cds.MS.gene04925.t1;Parent=MS.gene04925.t1 |
| chr3.4 | exon | 67865689 | 67866059 | 67865689 | ID=MS.gene04925.t1.exon2;Parent=MS.gene04925.t1 |
| chr3.4 | CDS | 67865689 | 67866059 | 67865689 | ID=cds.MS.gene04925.t1;Parent=MS.gene04925.t1 |
| Gene Sequence |
| Protein sequence |
