AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63370.t1 MTR_2g075300 67.579 475 143 3 1 475 1 464 0.0 660
MS.gene63370.t1 MTR_2g084535 59.259 486 174 8 7 474 5 484 0.0 580
MS.gene63370.t1 MTR_7g105400 41.767 498 234 9 9 474 11 484 1.98e-127 389
MS.gene63370.t1 MTR_7g105390 44.619 446 221 6 50 474 44 484 3.26e-125 384
MS.gene63370.t1 MTR_4g097590 40.700 457 232 8 53 474 55 507 1.83e-114 357
MS.gene63370.t1 MTR_7g105390 42.691 431 199 6 50 474 62 450 1.52e-110 344
MS.gene63370.t1 MTR_8g076210 28.861 395 225 15 57 428 17 378 9.67e-27 114
MS.gene63370.t1 MTR_5g021530 28.182 440 251 20 11 428 5 401 2.71e-24 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63370.t1 AT5G22860 51.494 435 193 7 52 474 53 481 8.37e-144 431
MS.gene63370.t1 AT5G22860 51.707 410 180 7 52 449 53 456 1.83e-133 404
MS.gene63370.t1 AT2G24280 43.210 486 241 8 10 474 5 476 1.80e-129 394
MS.gene63370.t1 AT5G22860 51.145 393 174 7 52 432 53 439 2.31e-125 382
MS.gene63370.t1 AT5G22860 51.145 393 174 7 52 432 53 439 2.31e-125 382
MS.gene63370.t1 AT5G65760 43.820 445 215 8 53 474 58 490 5.70e-119 368
MS.gene63370.t1 AT2G24280 42.117 444 222 8 10 432 5 434 1.04e-111 346
MS.gene63370.t1 AT5G22860 50.833 360 159 7 56 403 57 410 2.75e-109 342
MS.gene63370.t1 AT5G22860 50.833 360 159 7 56 403 57 410 2.75e-109 342
MS.gene63370.t1 AT5G22860 50.833 360 159 7 56 403 57 410 2.75e-109 342
MS.gene63370.t1 AT5G22860 50.949 316 138 6 52 356 53 362 1.54e-93 296
MS.gene63370.t1 AT3G28680 44.944 178 87 4 184 355 18 190 6.77e-42 151
MS.gene63370.t1 AT4G36195 29.204 452 255 21 1 428 1 411 7.49e-26 112
MS.gene63370.t1 AT4G36195 29.204 452 255 21 1 428 1 411 7.49e-26 112
MS.gene63370.t1 AT4G36195 29.204 452 255 21 1 428 1 411 7.66e-26 112
MS.gene63370.t1 AT2G18080 28.010 407 215 19 57 428 49 412 1.70e-23 105
MS.gene63370.t1 AT4G36190 26.915 457 259 19 1 428 1 411 1.83e-21 99.0

Find 204 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGCAAGTCTTGAATTTC+TGG 0.103087 2.4:+22039727 None:intergenic
GGATGTTCTGATTCATTTAA+AGG 0.152019 2.4:-22042178 MS.gene63370:CDS
AATGCTTCCCTTGATTTAAA+TGG 0.157015 2.4:+22042258 None:intergenic
TCATTGAAATTGAGGGTTTC+AGG 0.175778 2.4:+22042617 None:intergenic
GAGTCTGTTATGACAGTTTC+AGG 0.200001 2.4:+22037110 None:intergenic
TACAACATATCTTTGTTTAA+AGG 0.210814 2.4:+22042526 None:intergenic
TGAAGGGGTACAACTATTAT+TGG 0.217329 2.4:+22037143 None:intergenic
TGCCCCAATACTTGTCTCTT+TGG 0.234570 2.4:-22042471 MS.gene63370:CDS
ACAAATTCATATTACATTCT+TGG 0.237490 2.4:-22039800 MS.gene63370:CDS
TGTTGTAAATTTCAAGTATT+GGG 0.241494 2.4:-22042510 MS.gene63370:CDS
TCAATCAACTTGACTTCTTC+AGG 0.246564 2.4:+22036253 None:intergenic
TGTGGTTGTTGCTGTTGTTT+GGG 0.252353 2.4:+22037244 None:intergenic
TCACTATCAGTGTATTTATC+TGG 0.262447 2.4:+22039418 None:intergenic
AATTGAGGGTTTCAGGGTTT+TGG 0.285807 2.4:+22042624 None:intergenic
GCTCTTTGTGCAGGTGAAAT+TGG 0.304719 2.4:+22037302 None:intergenic
TTAGGTTTGGGTGCAGGTTT+TGG 0.311890 2.4:+22037380 None:intergenic
GATACAACATGTTTAGGTTT+GGG 0.321124 2.4:+22037368 None:intergenic
GATATCTGTGAGGGCATTAA+TGG 0.323021 2.4:-22039523 MS.gene63370:CDS
ATGAAGATGAAGATTGAATC+TGG 0.323660 2.4:-22037565 MS.gene63370:CDS
TGATGATAATTCAACAACTA+TGG 0.325869 2.4:-22036994 MS.gene63370:CDS
GCCCCAATACTTGTCTCTTT+GGG 0.330791 2.4:-22042470 MS.gene63370:CDS
CTCGATTGACTAACAATAGA+TGG 0.334500 2.4:+22037607 None:intergenic
GAGCGATGCAAGAAAGATTA+TGG 0.336880 2.4:-22039170 MS.gene63370:CDS
AGTTCTGATACAACATGTTT+AGG 0.337559 2.4:+22037362 None:intergenic
TTGTGGTTGTTGCTGTTGTT+TGG 0.339640 2.4:+22037243 None:intergenic
AGAAGTTACTGCTTTATATC+AGG 0.341333 2.4:-22037521 MS.gene63370:intron
TGATACAACATGTTTAGGTT+TGG 0.355940 2.4:+22037367 None:intergenic
AGAATCAGTTAGCTGCAGTT+AGG 0.368570 2.4:-22036180 MS.gene63370:CDS
CTTGAATTTCTGGCTTAGAA+TGG 0.372736 2.4:+22039737 None:intergenic
CAGTGCTTGCTACATGGTTT+CGG 0.373629 2.4:-22040072 MS.gene63370:CDS
GGAGTGGAGCTGACGAGGCC+AGG 0.378526 2.4:+22040020 None:intergenic
TGCAAAGGAATTTAACGGTT+GGG 0.380715 2.4:-22039389 MS.gene63370:CDS
TTGACTTCTTCAGGATCCTT+AGG 0.380760 2.4:+22036262 None:intergenic
CTCAAACTCTATGGTGTTTG+TGG 0.382594 2.4:+22037226 None:intergenic
CCCTCACAGATATCCGCAAC+TGG 0.392376 2.4:+22039532 None:intergenic
AAGGAATCAGGCCATTCATT+TGG 0.393296 2.4:-22036793 MS.gene63370:CDS
CACCCAAAGAGACAAGTATT+GGG 0.395483 2.4:+22042468 None:intergenic
CACTCAACTGTTGAAGCATC+AGG 0.395931 2.4:+22042675 None:intergenic
ATTAGAGAAAATAATGTTGC+TGG 0.399588 2.4:+22038983 None:intergenic
GATATCCTTAGTAGGATATA+CGG 0.401625 2.4:-22039484 MS.gene63370:CDS
CATTGAAATTGAGGGTTTCA+GGG 0.404798 2.4:+22042618 None:intergenic
ATTGATGCAAAGGAATTTAA+CGG 0.409107 2.4:-22039394 MS.gene63370:CDS
AGAGGGTGGCTCAAACTCTA+TGG 0.415273 2.4:+22037217 None:intergenic
CGTTCAGAGTTGCACGGAAA+TGG 0.416783 2.4:-22039261 MS.gene63370:intron
ATGTTGTAAATTTCAAGTAT+TGG 0.419169 2.4:-22042511 MS.gene63370:CDS
AAAGTCTTCATTGAAATTGA+GGG 0.419394 2.4:+22042610 None:intergenic
GAAAGTCTTCATTGAAATTG+AGG 0.422429 2.4:+22042609 None:intergenic
GAATCAGTTAGCTGCAGTTA+GGG 0.425506 2.4:-22036179 MS.gene63370:CDS
GAAACTGCAAGGAGACTTGT+TGG 0.427105 2.4:-22036230 MS.gene63370:CDS
CCACATCACCATCGGGGCCC+TGG 0.427669 2.4:-22040038 MS.gene63370:CDS
TTTCAGTTAGATAGTTGAAA+TGG 0.431390 2.4:+22042558 None:intergenic
CGTTACTTCTTGCAGTCCAT+TGG 0.432382 2.4:-22039623 MS.gene63370:intron
TATTTGCAAGCATGAGATCC+TGG 0.434627 2.4:+22038583 None:intergenic
ATTTAACGGTTGGGGTTGGC+AGG 0.437970 2.4:-22039380 MS.gene63370:intron
CAGGTGAAATTGGTTGATGT+TGG 0.438140 2.4:+22037312 None:intergenic
AATCAGTGCCATTTAAATCA+AGG 0.440026 2.4:-22042266 MS.gene63370:CDS
GCTAGTGGAATCAAGGAATC+AGG 0.441634 2.4:-22036805 MS.gene63370:CDS
AAGTCCCCGGTGATTGTGAT+TGG 0.441949 2.4:-22042138 MS.gene63370:CDS
TTCTTGAAGTTGAAAGGTTG+AGG 0.443975 2.4:+22039200 None:intergenic
AGTCCCCGGTGATTGTGATT+GGG 0.444479 2.4:-22042137 MS.gene63370:CDS
GGTTGGCTATGTGGATTTGA+AGG 0.445012 2.4:+22037035 None:intergenic
TGATATAAAGCAGTAACTTC+TGG 0.447141 2.4:+22037523 None:intergenic
TGAATTATCATCAGAGACTA+AGG 0.447682 2.4:+22037004 None:intergenic
CCAGGGCCCCGATGGTGATG+TGG 0.449045 2.4:+22040038 None:intergenic
ATCAGAGACTAAGGTAAGAA+TGG 0.449613 2.4:+22037013 None:intergenic
CCATGTTATACGTGACCCTC+AGG 0.450308 2.4:+22039575 None:intergenic
TTCTTGCAGTCCATTGGAAA+AGG 0.453113 2.4:-22039617 MS.gene63370:intron
GGAGGTATTGTGGCTGGCTA+TGG 0.454528 2.4:-22039463 MS.gene63370:CDS
GGCATTAATGGAGCTGCCTA+TGG 0.457456 2.4:-22039511 MS.gene63370:CDS
CTGGGGCTTCTGATGCTCCT+AGG 0.460524 2.4:-22037546 MS.gene63370:CDS
TTGAAGCATCAGGGATTTGA+TGG 0.461636 2.4:+22042685 None:intergenic
CCACCCAAAGAGACAAGTAT+TGG 0.461702 2.4:+22042467 None:intergenic
CAGGGCCCCGATGGTGATGT+GGG 0.461958 2.4:+22040039 None:intergenic
CTAATGGGCTAAAAGATCCT+TGG 0.462105 2.4:-22038965 MS.gene63370:CDS
AATATCCCCACATCACCATC+GGG 0.462616 2.4:-22040045 MS.gene63370:CDS
ATATAAAGTTGGTTCTCAAA+AGG 0.463493 2.4:-22039010 MS.gene63370:CDS
GTTTCTGTGATAACCAACTT+TGG 0.466566 2.4:+22036945 None:intergenic
GAGTGGAGCTGACGAGGCCA+GGG 0.470743 2.4:+22040021 None:intergenic
TCATCAAGATATTCCAAAGT+TGG 0.477476 2.4:-22036958 MS.gene63370:intron
GCAAAGGAATTTAACGGTTG+GGG 0.478149 2.4:-22039388 MS.gene63370:CDS
AAAGCAGTAACTTCTGGCCT+AGG 0.480115 2.4:+22037529 None:intergenic
GAACATCCGCGTAATCTTGA+AGG 0.480782 2.4:+22042193 None:intergenic
ACATGTTTAGGTTTGGGTGC+AGG 0.487189 2.4:+22037374 None:intergenic
TTGTTTAGTTGTTGTATCAA+AGG 0.488142 2.4:+22037280 None:intergenic
CCAATACTTGTCTCTTTGGG+TGG 0.490052 2.4:-22042467 MS.gene63370:CDS
GTGTGCAAGACTATCTTTGA+TGG 0.491729 2.4:-22036837 MS.gene63370:intron
TGAAGATGAAGATTGAATCT+GGG 0.497747 2.4:-22037564 MS.gene63370:CDS
TGCGTAATTAACCAAATGAA+TGG 0.500360 2.4:+22036782 None:intergenic
ACGTACATGTCCTCCGTAGC+AGG 0.501884 2.4:+22039118 None:intergenic
GTATCAAAGGCTCTTTGTGC+AGG 0.503378 2.4:+22037293 None:intergenic
TTGATTCCACTAGCTCCTCT+AGG 0.507030 2.4:+22036814 None:intergenic
ACGGAAATGGTGGCGCCCAT+TGG 0.509165 2.4:-22039248 MS.gene63370:CDS
AGAAGAACAGATAAGGAAGT+AGG 0.511566 2.4:-22036128 None:intergenic
TTCAAGAACACAAACACATT+GGG 0.511769 2.4:-22042237 MS.gene63370:CDS
TGTTATGACAGTTTCAGGAT+TGG 0.512182 2.4:+22037115 None:intergenic
CTTAGAATGGAAAGACCATT+CGG 0.513113 2.4:+22039750 None:intergenic
GCATAGTTCTTGAAGTTGAA+AGG 0.513354 2.4:+22039194 None:intergenic
TCAGAGACTAAGGTAAGAAT+GGG 0.519151 2.4:+22037014 None:intergenic
ATGGTGGCGCCCATTGGCAT+AGG 0.519789 2.4:-22039242 MS.gene63370:CDS
TTTCAGGATTGGCAAACTGA+AGG 0.520149 2.4:+22037126 None:intergenic
TAGAGGAGCTAGTGGAATCA+AGG 0.523322 2.4:-22036812 MS.gene63370:CDS
ACTATGCAGTGCTTGCTACA+TGG 0.523584 2.4:-22040078 MS.gene63370:intron
TCTTGTTAGCATCGGTACTA+CGG 0.524106 2.4:-22042291 MS.gene63370:intron
TTCAGGATTGGCAAACTGAA+GGG 0.524117 2.4:+22037127 None:intergenic
GCATTATCCGTCATAAAATC+CGG 0.524995 2.4:+22042422 None:intergenic
CTAAAAGATCCTTGGAGTAG+TGG 0.525390 2.4:-22038957 MS.gene63370:CDS
GGGCTACTTAAACGCAGCGC+AGG 0.525535 2.4:-22042217 MS.gene63370:CDS
ATGATGTATCAGAGACTGAC+TGG 0.525984 2.4:+22037183 None:intergenic
ATGCAAAGGAATTTAACGGT+TGG 0.530074 2.4:-22039390 MS.gene63370:CDS
GAAGAATTCCCTATGCCAAT+GGG 0.531114 2.4:+22039233 None:intergenic
TAATTGCCAGGATATAAAGT+TGG 0.531504 2.4:-22039021 MS.gene63370:intron
ATGGAGATGATATCCTTAGT+AGG 0.531817 2.4:-22039492 MS.gene63370:CDS
GTGGCCCTTCCGACGATCAA+TGG 0.532452 2.4:-22038816 MS.gene63370:intron
AAATATCCCCACATCACCAT+CGG 0.533495 2.4:-22040046 MS.gene63370:CDS
CCTCACAGATATCCGCAACT+GGG 0.533634 2.4:+22039533 None:intergenic
GTAAGAATGGGTTGGCTATG+TGG 0.534872 2.4:+22037026 None:intergenic
TGACTGGGTGGATGAGATAG+AGG 0.535637 2.4:+22037199 None:intergenic
ATTTGCAAGCATGAGATCCT+GGG 0.540218 2.4:+22038584 None:intergenic
AAGTAGAGCTTGCAAGGAAG+CGG 0.540251 2.4:+22042400 None:intergenic
ATCAGTGCCATTTAAATCAA+GGG 0.544935 2.4:-22042265 MS.gene63370:CDS
AGGAATTTAACGGTTGGGGT+TGG 0.548262 2.4:-22039384 MS.gene63370:CDS
AAAGAAAGGTTGACCAATGT+GGG 0.548744 2.4:-22037639 MS.gene63370:intron
GTCCCCGGTGATTGTGATTG+GGG 0.549779 2.4:-22042136 MS.gene63370:CDS
TATCAAGAGACTAGATCCTA+AGG 0.551435 2.4:-22036278 MS.gene63370:CDS
GGAAGAATTCCCTATGCCAA+TGG 0.553453 2.4:+22039232 None:intergenic
CATTCGGCTGAGACGCTACT+CGG 0.553610 2.4:+22039766 None:intergenic
GTTGTAAATTTCAAGTATTG+GGG 0.553624 2.4:-22042509 MS.gene63370:CDS
TTTATCACTTCCTGCTACGG+AGG 0.553697 2.4:-22039128 MS.gene63370:CDS
TACCTCCGTATATCCTACTA+AGG 0.556731 2.4:+22039479 None:intergenic
TGACGAGGCCAGGGCCCCGA+TGG 0.559413 2.4:+22040030 None:intergenic
TGGTTAATTACGCAGACAAG+TGG 0.559552 2.4:-22036773 MS.gene63370:CDS
TAGCAGGAAGTGATAAAATG+AGG 0.561200 2.4:+22039134 None:intergenic
AGACTAAGGTAAGAATGGGT+TGG 0.561487 2.4:+22037018 None:intergenic
ATTCAAGAACACAAACACAT+TGG 0.562139 2.4:-22042238 MS.gene63370:CDS
GTACACAAGTAGAGCTTGCA+AGG 0.563319 2.4:+22042394 None:intergenic
ATATACGGAGGTATTGTGGC+TGG 0.564129 2.4:-22039469 MS.gene63370:CDS
TAAAGAAAGGTTGACCAATG+TGG 0.571134 2.4:-22037640 MS.gene63370:intron
ATTAGACGCGCAGAAGTCCC+CGG 0.572087 2.4:-22042151 MS.gene63370:CDS
GTTGGCTATGTGGATTTGAA+GGG 0.572160 2.4:+22037036 None:intergenic
TAGGATATACGGAGGTATTG+TGG 0.572161 2.4:-22039473 MS.gene63370:CDS
TAATTCAAGAAGAACAGATA+AGG 0.577878 2.4:-22036135 MS.gene63370:CDS
TGATAGTGATATTGATGCAA+AGG 0.581365 2.4:-22039404 MS.gene63370:CDS
GTAGAGGAGTGGAGCTGACG+AGG 0.584668 2.4:+22040015 None:intergenic
AGTTGAGTGTATTGTCAGCA+TGG 0.584815 2.4:-22042661 MS.gene63370:CDS
TGGTGGCGCCCATTGGCATA+GGG 0.585572 2.4:-22039241 MS.gene63370:CDS
ATGGATGGTGGAACCCACAT+TGG 0.586636 2.4:+22037626 None:intergenic
TCCCCGGTGATTGTGATTGG+GGG 0.591156 2.4:-22042135 MS.gene63370:CDS
GACTATCTTTGATGGCCTAG+AGG 0.592466 2.4:-22036829 MS.gene63370:CDS
TCTTTGATCAACTAACCACC+CGG 0.592846 2.4:+22038551 None:intergenic
AAGTGAAATCGAATATTCGC+CGG 0.593816 2.4:-22042441 MS.gene63370:CDS
TCAGTTAGCTGCAGTTAGGG+AGG 0.594703 2.4:-22036176 MS.gene63370:CDS
GTGTGATACTATCGAAGTAG+AGG 0.596110 2.4:+22039999 None:intergenic
AAAGATCCTTGGAGTAGTGG+AGG 0.598756 2.4:-22038954 MS.gene63370:CDS
CTCACAGATATCCGCAACTG+GGG 0.599177 2.4:+22039534 None:intergenic
GTAAATTTCAAGTATTGGGG+TGG 0.599707 2.4:-22042506 MS.gene63370:CDS
GAAGATGAAGATTGAATCTG+GGG 0.599986 2.4:-22037563 MS.gene63370:CDS
ACTAAGGATATCATCTCCAT+AGG 0.600675 2.4:+22039495 None:intergenic
GAAAGGTTGAGGTTGAAACA+TGG 0.600936 2.4:+22039211 None:intergenic
GCATACCATTGATCGTCGGA+AGG 0.600987 2.4:+22038811 None:intergenic
AGTTGATCAAAGAAAGCAAG+AGG 0.603484 2.4:-22038541 MS.gene63370:intron
TCAGGATTGGCAAACTGAAG+GGG 0.604635 2.4:+22037128 None:intergenic
CCTGAGGGTCACGTATAACA+TGG 0.605375 2.4:-22039575 MS.gene63370:CDS
ATTGACTAACAATAGATGGA+TGG 0.606516 2.4:+22037611 None:intergenic
TCAGAGTTGCACGGAAATGG+TGG 0.607104 2.4:-22039258 MS.gene63370:intron
AGCTCTACTTGTGTACATTG+AGG 0.608262 2.4:-22042387 MS.gene63370:intron
AGGGCCCCGATGGTGATGTG+GGG 0.609240 2.4:+22040040 None:intergenic
GTGGGAGAACTTGAGACAGT+TGG 0.613052 2.4:-22036754 MS.gene63370:intron
AGCAGGAAGTGATAAAATGA+GGG 0.616183 2.4:+22039135 None:intergenic
AACATCCGCGTAATCTTGAA+GGG 0.617142 2.4:+22042194 None:intergenic
CCAGTTGCGGATATCTGTGA+GGG 0.619242 2.4:-22039532 MS.gene63370:CDS
AAGATCCTTGGAGTAGTGGA+GGG 0.619362 2.4:-22038953 MS.gene63370:intron
CGAGTAGCGTCTCAGCCGAA+TGG 0.620320 2.4:-22039765 MS.gene63370:CDS
GACTAACAATAGATGGATGG+TGG 0.620667 2.4:+22037614 None:intergenic
TCACAGATATCCGCAACTGG+GGG 0.623202 2.4:+22039535 None:intergenic
ATTACACGTTCAGAGTTGCA+CGG 0.624817 2.4:-22039267 MS.gene63370:intron
TGATGGCCTAGAGGAGCTAG+TGG 0.626712 2.4:-22036820 MS.gene63370:CDS
ACTCAACTGTTGAAGCATCA+GGG 0.627053 2.4:+22042676 None:intergenic
GGTTAATTACGCAGACAAGT+GGG 0.629434 2.4:-22036772 MS.gene63370:CDS
GTGGTTGTTGCTGTTGTTTG+GGG 0.630545 2.4:+22037245 None:intergenic
CATGTTATACGTGACCCTCA+GGG 0.631235 2.4:+22039576 None:intergenic
CATACCATTGATCGTCGGAA+GGG 0.631836 2.4:+22038812 None:intergenic
TATTGTTAGTCAATCGAGTG+AGG 0.636723 2.4:-22037602 MS.gene63370:CDS
AAGCATCAGGGATTTGATGG+TGG 0.637086 2.4:+22042688 None:intergenic
TGATTTATCAGATAGTCTTG+TGG 0.643629 2.4:-22038835 MS.gene63370:CDS
GAAGTGATAAAATGAGGGCG+AGG 0.644408 2.4:+22039140 None:intergenic
CCCAGTTGCGGATATCTGTG+AGG 0.648176 2.4:-22039533 MS.gene63370:CDS
CACAGATATCCGCAACTGGG+GGG 0.655013 2.4:+22039536 None:intergenic
ACTCATGACTGATGCATCAG+AGG 0.657955 2.4:-22037081 MS.gene63370:CDS
AAGTTGATTGAGAAACTGCA+AGG 0.658398 2.4:-22036241 MS.gene63370:CDS
ATACTATCGAAGTAGAGGAG+TGG 0.659406 2.4:+22040004 None:intergenic
CTTTGATCAACTAACCACCC+GGG 0.659576 2.4:+22038552 None:intergenic
TGGTGGTAACAAAGAAACAC+AGG 0.665359 2.4:+22042705 None:intergenic
ACCCAAAGAGACAAGTATTG+GGG 0.666185 2.4:+22042469 None:intergenic
GACTGGGTGGATGAGATAGA+GGG 0.674656 2.4:+22037200 None:intergenic
GTTTACCCTCCACTACTCCA+AGG 0.674807 2.4:+22038948 None:intergenic
ATAAGTTGAAAGAGTCCCTG+AGG 0.681664 2.4:-22039591 MS.gene63370:CDS
ACATGCATACCATTGATCGT+CGG 0.682623 2.4:+22038807 None:intergenic
TGATGTATCAGAGACTGACT+GGG 0.690008 2.4:+22037184 None:intergenic
TAAGTTGAAAGAGTCCCTGA+GGG 0.701964 2.4:-22039590 MS.gene63370:CDS
ATCCTTAGTAGGATATACGG+AGG 0.710424 2.4:-22039481 MS.gene63370:CDS
TGGGTGGATGAGATAGAGGG+TGG 0.720660 2.4:+22037203 None:intergenic
GCAGGCCCTTCAAGATTACG+CGG 0.737103 2.4:-22042199 MS.gene63370:CDS
TGTATCAGAGACTGACTGGG+TGG 0.747740 2.4:+22037187 None:intergenic
ATATCCCCACATCACCATCG+GGG 0.767234 2.4:-22040044 MS.gene63370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.4 gene 22036129 22042768 22036129 ID=MS.gene63370
chr2.4 mRNA 22036129 22042768 22036129 ID=MS.gene63370.t1;Parent=MS.gene63370
chr2.4 exon 22042388 22042768 22042388 ID=MS.gene63370.t1.exon1;Parent=MS.gene63370.t1
chr2.4 CDS 22042388 22042768 22042388 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22042121 22042304 22042121 ID=MS.gene63370.t1.exon2;Parent=MS.gene63370.t1
chr2.4 CDS 22042121 22042304 22042121 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22039958 22040091 22039958 ID=MS.gene63370.t1.exon3;Parent=MS.gene63370.t1
chr2.4 CDS 22039958 22040091 22039958 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22039729 22039841 22039729 ID=MS.gene63370.t1.exon4;Parent=MS.gene63370.t1
chr2.4 CDS 22039729 22039841 22039729 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22039381 22039630 22039381 ID=MS.gene63370.t1.exon5;Parent=MS.gene63370.t1
chr2.4 CDS 22039381 22039630 22039381 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22039124 22039276 22039124 ID=MS.gene63370.t1.exon6;Parent=MS.gene63370.t1
chr2.4 CDS 22039124 22039276 22039124 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22038954 22039033 22038954 ID=MS.gene63370.t1.exon7;Parent=MS.gene63370.t1
chr2.4 CDS 22038954 22039033 22038954 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22038817 22038866 22038817 ID=MS.gene63370.t1.exon8;Parent=MS.gene63370.t1
chr2.4 CDS 22038817 22038866 22038817 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22038542 22038615 22038542 ID=MS.gene63370.t1.exon9;Parent=MS.gene63370.t1
chr2.4 CDS 22038542 22038615 22038542 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22037522 22037653 22037522 ID=MS.gene63370.t1.exon10;Parent=MS.gene63370.t1
chr2.4 CDS 22037522 22037653 22037522 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22036959 22037411 22036959 ID=MS.gene63370.t1.exon11;Parent=MS.gene63370.t1
chr2.4 CDS 22036959 22037411 22036959 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22036755 22036850 22036755 ID=MS.gene63370.t1.exon12;Parent=MS.gene63370.t1
chr2.4 CDS 22036755 22036850 22036755 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
chr2.4 exon 22036129 22036305 22036129 ID=MS.gene63370.t1.exon13;Parent=MS.gene63370.t1
chr2.4 CDS 22036129 22036305 22036129 ID=cds.MS.gene63370.t1;Parent=MS.gene63370.t1
Gene Sequence

>MS.gene63370

ATGGAGAAGTTTATATCTTTTAATCTTCTCTGCATATTCTTCCTGTGTTTCTTTGTTACCACCATCAAATCCCTGATGCTTCAACAGTTGAGTGTATTGTCAGCATGGAAAACTATCAAGTACCAAAACCCTGAAACCCTCAATTTCAATGAAGACTTTCATACATATTTTTACAAACAAACTTTAGACCATTTCAACTATCTAACTGAAACCTCTAAAACCTTTAAACAAAGATATGTTGTAAATTTCAAGTATTGGGGTGGTGCTAATTCTAGTGCCCCAATACTTGTCTCTTTGGGTGGAGAAAGTGAAATCGAATATTCGCCGGATTTTATGACGGATAATGCCGCTTCCTTGCAAGCTCTACTTGTGTACATTGAGGTAATAAAGACGACTATTTCTATTAACACTCAATGATCTAAATCAAGTTAGTTTTCTGATTATGCTCTTAATTTTCTTGTTAGCATCGGTACTACGGAAAATCAGTGCCATTTAAATCAAGGGAAGCATTCAAGAACACAAACACATTGGGCTACTTAAACGCAGCGCAGGCCCTTCAAGATTACGCGGATGTTCTGATTCATTTAAAGGCTTCATTAGACGCGCAGAAGTCCCCGGTGATTGTGATTGGGGGATCATACGCTGGAAGTAAGTTTCCAAATCATGTTTCTCTTTTTATTCACTTTTTATCATCTATTTCTGCTTGTTCCATTTTTTTTTACTCCTTCTATGTTTAAACTTTTAAATTATGTTTTCTTTTAATTTTATTTTTCTATTTTGTGTTTCTCTTATTTTTGTTATTAAAATTATGCTGTTATCATCATTCCATAATTTTTAACGTTGAATTTCAATTATGTAGTATTTGAATTCTAATTCTATTTTTTCTTTTTGTTTTCTCTAATTTTTATTTGTAAGTTGCTTTTTCATTTATTTTTCTTGTTCTTTGAATTATACTTTTATTTTAATTTTATCTTCTAGTTGTATAATTTTGGTTTATATTTTTCTAATTTATTTTTGCCACCTTTCTTTAAATATTGAACCAACTATTTTGCATTTGCAGTATTAAACTTGATGGTCATTTGTAGTTTTACTTGGCTATGCGTCCATAATATATATATATATGGTGTATTTTTTTGGTGACCTCTAATCTGCCAATCATCAAATTGATAAAAGCTTAAGTCGTTGTTGCAAACTATTTTTTTATCAAGATTACTCCTGATACATACATTAGAAAGACATCTTTTGTAAAGACTAGACAAGTATTGGTATTTTACCATTCTAGAAAGATATAATCATACAAAGACAAAGTCTAATTGTCTTGATCATGTGAGCTAATTATGCAGCAAAGGACAGTGAACCAACAACTTAAAATTTCAGCAGGTTTGTAGCCAATGCTTAATTTCCCTGGGTGTGTAATTAGACCCCATATGATCACAGTCCTTGACCAATGCCATAAAACGTTATACTACTAAAACCATCCGGCAACTATCTCCTATAGCAAATGCATCTTTTCTAAGGGCGGCCACTTTTTTGTCAGTGAGAGTGTTCACCCTTTTATTCAACTACTGTTTATTCCTATTAATAGAAAACTGTAAAGTGATGAATCTAGAGAGCTTCTAGCTTCTCTGAAATAATTGTTTTTGTGTTTTCTGTTAGAATGATTTTCATTGTGATTGCAAAAGATAGATGAGTCTATTTATAGACATGTGTTTGCTACTCTATCTGACTGACAACTTTCAGACAGCAATCCCGGCGAGACAGTTTATTGCCAGCTCATACAGTGAGGCAGTGACACAATTCTGTTAGACAGTTACAGAGGGAGAGTAATCTAGTGTGATAACCCTATTGTGATCATTCTGATCACAAGCTAATATATGCTACGCTATATCTTCAATACCCCCCTGCAAGCTTAACCAGGAGTGAGCAATCGCATATAGTTAGCTTGAAGCGTAGGTCTTCAAATCTAGATGGAGGAAGAGCCTCGGTCATGATATCTGCATGTCAGAGCTGGCCTGGAATGTGAGTGACCAATAACTGCTTCTGAATGACCTTCTCTCTGACAAAGAACAGGTCTAATTCCATGTGCTTGGTACAAGCATAGAGTACAGGATTATGAGCAAGAGCAATGGTGCTCATATTGTCACAGAATACTTTATTTGTGCTGTAGGGAACACCTAGCTCAGTAAGAAGAGACTGAACCCACAAAACCACCAGGAAATGATGTTAGGGCCCAGGAAAACACAGGCACCTGAGTTGGACCTTCTGTCATCAGGTTGTGAGCCCGAATCAGCATCACAGTAGGCATAAAGAGAGATAGGTATGAAAGGCATTGGTTGCAGATCAAGGCCTCAAGTGATAGTGCCTTTAAGGTATCTTAAGATATGTTTGGGGGTGCAGACCAGTGAGCATCCAGAGTGGTCATAAATTGGCATACTTTATTGACAGCAAAACATCTGTCTGGTCAGGTCTGGTAACATATTGAAGGGCACCCACAATTGATCTATATAGAGTAGGATCAGAGAATGGAGAAAAGCCTTCTTTGGAAAGTTTGATTCCAGAAACCATAGGAGAGGGGAGAGATTTAGCTTCATGCATGATTTTTCTAGTGAGCAAATATCTTCAATACTTGTATATGGTTTTCTCTTAATTAATGTTTATATATTTGTTAACTATGCAGTGCTTGCTACATGGTTTCGGCTGAAATATCCCCACATCACCATCGGGGCCCTGGCCTCGTCAGCTCCACTCCTCTACTTCGATAGTATCACACCAGAAAATGCGTACTTTGATGTTATTACACGAGATTTCAAAGTAATTCACGATAGTTTTTTCTTTTTTCATCTTTCATATTATTTGATTTATTATTATTTTTTTTTCAAAAACATAATTAAGATGGTTTGTTTCTAAATCAATTTATTGGTGTGCAGGACACTAGTGAGACATGCTACAAATTCATATTACATTCTTGGTCTGAAATTGACCGAGTAGCGTCTCAGCCGAATGGTCTTTCCATTCTAAGCCAGAAATTCAAGACTTGCCAGTAAAAACAAATTTTCAATTTATTTCCTCCCTTTTGTTTTGATTGAACTAGGATTTTAAGTGCAGTGTTTAATTTACTCAAACCGTTACTTCTTGCAGTCCATTGGAAAAGGCTGATAAGTTGAAAGAGTCCCTGAGGGTCACGTATAACATGGCAGCTCAATACAATTACCCCCCAGTTGCGGATATCTGTGAGGGCATTAATGGAGCTGCCTATGGAGATGATATCCTTAGTAGGATATACGGAGGTATTGTGGCTGGCTATGGAAACATCACATGCGATGATAATCCAGATAAATACACTGATAGTGATATTGATGCAAAGGAATTTAACGGTTGGGGTTGGCAGGTAATCACATTTTAATTGCAGTCCTTATATGAAAGAGTAAATAATGAATTATTACTTTGTATATCTTGTTGATCTAATAATAAATTGAATTATTACACGTTCAGAGTTGCACGGAAATGGTGGCGCCCATTGGCATAGGGAATTCTTCCATGTTTCAACCTCAACCTTTCAACTTCAAGAACTATGCTGAGCGATGCAAGAAAGATTATGGAGTATCACCTCGCCCTCATTTTATCACTTCCTGCTACGGAGGACATGTACGTAGTTTTATAACATTTAGATATCAATTTCACTATTGAGATTGTATTAACAGCACAATTAATTTCATCATTGTGACTAATTGCCAGGATATAAAGTTGGTTCTCAAAAGGTTTGCCAGCAACATTATTTTCTCTAATGGGCTAAAAGATCCTTGGAGTAGTGGAGGGTAAACACATAAAATCCAATAAATAAATTCATCTTGGTTATTTTAGAACAATGTTTTAAAATAAATTGTGGCTGTTACATATTTCAGGGTTCTGCATGATTTATCAGATAGTCTTGTGGCCCTTCCGACGATCAATGGTATGCATGTCTTGGTATTGGGTGAATACACACAATTTAGTTGTCTATTTATATTATATAATAAAAATTATAATATAAATAAATAGAGAACACATTGTAGTAGTATTAAGAGTACCTTGCTGATTTTTATCATTTTATATCAAAATGTAAACCAATTAATCAAATGGTTATGTAAATGTTTAACAAATGTTTATTTTTCAGGAACTCATACCCAGGATCTCATGCTTGCAAATAAAAATGATCCCGGGTGGTTAGTTGATCAAAGAAAGCAAGAGGTAGAGATAATCAAAAGATGGATTGCACAGTATTACGCTGATAGAGCATGAAGTTAAGATCTAATAAGGGGATGTGCCTGTGAATTGTTAGAAAGATGGTGCTTTTTAAGTCGTAGTTCCTCTAAGGTTAAAAATGGAATCTCCTTCAAAAAGATCAGAATGTTAAAAATATATTGTGTTCGTGTTATCTACTACTGAAGAATTACAGTTTGATATAATATGTGGGGATGATGGTTTACGTGACAAAAGTTACACCTACTATTTATCAAAACCAAAGTTACACCTATTTTATGTGACTTCATTTGAAATAATACCCGAGTGTGTGATTATTGTTATGGTTTAATTTTAGGAAAATGGTTATGTCCCTTAGGGTTGTGGATTTTAGGTTAATTGATGAAATTTTTATGTCATATATGTGAAGAGAGAATTTAAAATATTTTTTCTGCTAAAAAAATTGACATAGTTGGATTAAAATAATGGGAGCTAGAAATAAGGTTTTGAGTGAAATCAATTGGATTGGAATGTTAGTTTGCGGTGCTTTCTTATTTAGATCTTTCTTGAGTGAAACAAATTTGAGTTTGACAGATTAATTAAAATACACTTTGACTGATAAGCTTGTACTAAGGTTTATACTTATATGTTTATGTAGTTTATGAATTTTGTATGCCTCTAAATATAAAAATGAATTTGATTATTATAATAATACAAATACAGATACCAAGAGTCTTGAGATAAATGAATTTAACATATGATCACGATAAGGTCAATGTGGATACTTTAACACTAAAAGGAGATGAAAAAACTTTGCCTTGGTGATAATATGGATTCAGATTTGCTAAAATGATCCAACGCTTTAGAAAAAGTCAAAATGTTATATTGTAAAGAAAGGTTGACCAATGTGGGTTCCACCATCCATCTATTGTTAGTCAATCGAGTGAGGTTCAGAAGACTCAAATGAAGATGAAGATTGAATCTGGGGCTTCTGATGCTCCTAGGCCAGAAGTTACTGCTTTATATCAGGTAATCTTGCACACTCTTCACCCTCCTAACCCATTAACATAGACTCATCTTCAGACTCTGAAGATGAAGTACCTCTTAGCCAAAAATACACCGATCTACAAAAAGTTAAGACCCCCTCACCAAAACCTGCACCCAAACCTAAACATGTTGTATCAGAACTATGCACTGATATCATAGTTTCAGAAATCCAACATCAACCAATTTCACCTGCACAAAGAGCCTTTGATACAACAACTAAACAAGTTGTATCAGAACCCCAAACAACAGCAACAACCACAAACACCATAGAGTTTGAGCCACCCTCTATCTCATCCACCCAGTCAGTCTCTGATACATCATCAGTATCAGAAGACAAACCAATAATAGTTGTACCCCTTCAGTTTGCCAATCCTGAAACTGTCATAACAGACTCTGATGTACTCATGACTGATGCATCAGAGGATGTAAGTATGCTTGTAGCTAACCCTTCAAATCCACATAGCCAACCCATTCTTACCTTAGTCTCTGATGATAATTCAACAACTATGGAAATATCAAATGTTCATCAAGATATTCCAAAGTTGGTTATCACAGAAACCCAAACAATTCCAACAACCTGAACCTCTTAACAAATAACCATCTCACCCCCTCCAACCTTAACCTCTAACATAGTTCTGAGAGAGGTGTGCAAGACTATCTTTGATGGCCTAGAGGAGCTAGTGGAATCAAGGAATCAGGCCATTCATTTGGTTAATTACGCAGACAAGTGGGAGAACTTGAGACAGTTGGTAAATAAGAGACTTGATGACCTTCAATCCATCTATATTCAAGCTTAAGATGCAACTTTGAGAAAATGGTTTAAGAAAGTTGTGCAGAGTATGGAAGCAGTTTGAGCTCAAAAAGTCAGTCAAGCAACTGTCAGACACTCCTCAATATCCTGATCCTACTAGGGAGATTCCTCAAATTGTCAAGAATGGCCTTGATACCTCCTGGATAACAAGCATGTTCATCAAACCTTCCCAATAGCCGCAGGTTCCTTAATTTTCTTATTCCCTCATAGGGGAAATAAGGTTATTAGGTGGTATAACATTTGTATATGCTTAATCGATCTTCTTCTAACAACCTATGCATTTTGTTATCATTTCCAATTAGATGATCCACTAATTCAACTGACAGAGAATTATAAATGGATCAATTTTTTTTATTTTTACCGGAGATTGGGAATAGATTGGAGAAGGTGGTTATCAAGAGACTAGATCCTAAGGATCCTGAAGAAGTCAAGTTGATTGAGAAACTGCAAGGAGACTTGTTGGATCAGAAAATGATTTCTGAAGATTTGAAGAATCAGTTAGCTGCAGTTAGGGAGGAACAGAAGAGACAGATTATAATTCAAGAAGAACAGATAAGGAAGTAG
Protein sequence

>MS.gene63370.t1

MEKFISFNLLCIFFLCFFVTTIKSLMLQQLSVLSAWKTIKYQNPETLNFNEDFHTYFYKQTLDHFNYLTETSKTFKQRYVVNFKYWGGANSSAPILVSLGGESEIEYSPDFMTDNAASLQALLVYIEHRYYGKSVPFKSREAFKNTNTLGYLNAAQALQDYADVLIHLKASLDAQKSPVIVIGGSYAGMLATWFRLKYPHITIGALASSAPLLYFDSITPENAYFDVITRDFKDTSETCYKFILHSWSEIDRVASQPNGLSILSQKFKTCHPLEKADKLKESLRVTYNMAAQYNYPPVADICEGINGAAYGDDILSRIYGGIVAGYGNITCDDNPDKYTDSDIDAKEFNGWGWQSCTEMVAPIGIGNSSMFQPQPFNFKNYAERCKKDYGVSPRPHFITSCYGGHDIKLVLKRFASNIIFSNGLKDPWSSGGVLHDLSDSLVALPTINGTHTQDLMLANKNDPGWLVDQRKQEVDQCGFHHPSIVSQSSEVQKTQMKMKIESGASDAPRPEVTALYQTPSPKPAPKPKHVVSELCTDIIVSEIQHQPISPAQRAFDTTTKQVVSEPQTTATTTNTIEFEPPSISSTQSVSDTSSVSEDKPIIVVPLQFANPETVITDSDVLMTDASEDVSMLVANPSNPHSQPILTLVSDDNSTTMEISNVHQDIPKLTIFDGLEELVESRNQAIHLVNYADKWENLRQLVVIKRLDPKDPEEVKLIEKLQGDLLDQKMISEDLKNQLAAVREEQKRQIIIQEEQIRK