AlfalfaGEDB | MS.gene63458

M. sativa cultivar XinJiangDaYe / MS.gene63458

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63458.t1	XP_003620539.1	97.8	90	2	0	1	90	195	284	4.90E-43	183.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63458.t1	A0A109QYD3	48.1	81	42	0	1	81	182	262	6.9e-14	77.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63458.t1	G7KNK3	97.8	90	2	0	1	90	195	284	3.5e-43	183.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050426	MS.gene63458	-0.821391	4.14E-53	-1.69E-46
MS.gene052011	MS.gene63458	-0.803533	3.33E-49	-1.69E-46
MS.gene052077	MS.gene63458	0.827856	1.24E-54	-1.69E-46
MS.gene052420	MS.gene63458	0.857807	1.27E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63458.t1	MTR_6g086560	97.778	90	2	1	90	195	284	9.03e-60	184
MS.gene63458.t1	MTR_3g045440	60.000	85	34	1	85	185	269	2.71e-29	105
MS.gene63458.t1	MTR_1g018320	45.679	81	44	1	81	183	263	7.12e-19	78.2
MS.gene63458.t1	MTR_5g016150	40.000	80	48	1	80	181	260	7.51e-16	70.5
MS.gene63458.t1	MTR_4g095310	41.096	73	43	1	73	181	253	2.96e-14	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63458.t1	AT3G24420	52.874	87	40	1	1	86	187	273	2.00e-24	92.8
MS.gene63458.t1	AT3G03990	42.857	84	48	0	1	84	182	265	1.40e-17	74.7
MS.gene63458.t1	AT4G37470	36.250	80	51	0	1	80	181	260	3.78e-13	62.8

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACATGGAGAAGAAAATTAA+AGG	0.264049	6.3:-79363307	MS.gene63458:CDS
TTGGAGATAATTGACACTTT+TGG	0.295294	6.3:-79363274	MS.gene63458:CDS
ATGTAAAGAGCTACACTATA+TGG	0.336358	6.3:+79363325	None:intergenic
TCAGCTGGTTGATGTGCTTA+AGG	0.340672	6.3:-79363224	MS.gene63458:CDS
CAGCTGGTTGATGTGCTTAA+GGG	0.382841	6.3:-79363223	MS.gene63458:CDS
ACATGGAGAAGAAAATTAAA+GGG	0.433639	6.3:-79363306	MS.gene63458:CDS
AATTAAAGGGAAAGTTACAT+TGG	0.494880	6.3:-79363293	MS.gene63458:CDS
AGCACATCAACCAGCTGAAG+TGG	0.517198	6.3:+79363229	None:intergenic
ATATAGTGTAGCTCTTTACA+TGG	0.517253	6.3:-79363323	MS.gene63458:CDS
ACTAACTGCTCCACTTCAGC+TGG	0.538136	6.3:-79363239	MS.gene63458:CDS
GAGGTTTGAATAATTGTGCA+TGG	0.540705	6.3:+79363364	None:intergenic
GTCACCGCAAAACACTGTCT+TGG	0.557039	6.3:+79363417	None:intergenic
GCAAAACACTGTCTTGGCTA+AGG	0.590734	6.3:+79363423	None:intergenic
ACTGTCTTGGCTAAGGACAA+TGG	0.594937	6.3:+79363430	None:intergenic
TGAAGTGGAGCAGTTAGTTG+AGG	0.612568	6.3:+79363244	None:intergenic
TATTCAAACCTCTAGTGACA+TGG	0.628354	6.3:-79363353	MS.gene63458:CDS
TGGAACAACCATGTCACTAG+AGG	0.653480	6.3:+79363345	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATTAAAGGGAAAGTTACAT+TGG	-	chr6.3:79363347-79363366	MS.gene63458:CDS	25.0%
!	ACATGGAGAAGAAAATTAAA+GGG	-	chr6.3:79363334-79363353	MS.gene63458:CDS	25.0%
!	TACATGGAGAAGAAAATTAA+AGG	-	chr6.3:79363333-79363352	MS.gene63458:CDS	25.0%
	ATATAGTGTAGCTCTTTACA+TGG	-	chr6.3:79363317-79363336	MS.gene63458:CDS	30.0%
	ATGTAAAGAGCTACACTATA+TGG	+	chr6.3:79363318-79363337	None:intergenic	30.0%
!	TTGGAGATAATTGACACTTT+TGG	-	chr6.3:79363366-79363385	MS.gene63458:CDS	30.0%
	GAGGTTTGAATAATTGTGCA+TGG	+	chr6.3:79363279-79363298	None:intergenic	35.0%
	TATTCAAACCTCTAGTGACA+TGG	-	chr6.3:79363287-79363306	MS.gene63458:CDS	35.0%
!!!	ATGTGCTTAAGGGTGTTTTA+GGG	-	chr6.3:79363427-79363446	MS.gene63458:CDS	35.0%
!	TTAGCCAAGACAGTGTTTTG+CGG	-	chr6.3:79363219-79363238	MS.gene63458:CDS	40.0%
!!!	GATGTGCTTAAGGGTGTTTT+AGG	-	chr6.3:79363426-79363445	MS.gene63458:CDS	40.0%
	ACTGTCTTGGCTAAGGACAA+TGG	+	chr6.3:79363213-79363232	None:intergenic	45.0%
	GCAAAACACTGTCTTGGCTA+AGG	+	chr6.3:79363220-79363239	None:intergenic	45.0%
	TGAAGTGGAGCAGTTAGTTG+AGG	+	chr6.3:79363399-79363418	None:intergenic	45.0%
	TGGAACAACCATGTCACTAG+AGG	+	chr6.3:79363298-79363317	None:intergenic	45.0%
!	CAGCTGGTTGATGTGCTTAA+GGG	-	chr6.3:79363417-79363436	MS.gene63458:CDS	45.0%
!	TCAGCTGGTTGATGTGCTTA+AGG	-	chr6.3:79363416-79363435	MS.gene63458:CDS	45.0%
	ACTAACTGCTCCACTTCAGC+TGG	-	chr6.3:79363401-79363420	MS.gene63458:CDS	50.0%
	AGCACATCAACCAGCTGAAG+TGG	+	chr6.3:79363414-79363433	None:intergenic	50.0%
	GTCACCGCAAAACACTGTCT+TGG	+	chr6.3:79363226-79363245	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	79363195	79363467	79363195	ID=MS.gene63458
chr6.3	mRNA	79363195	79363467	79363195	ID=MS.gene63458.t1;Parent=MS.gene63458
chr6.3	exon	79363195	79363467	79363195	ID=MS.gene63458.t1.exon1;Parent=MS.gene63458.t1
chr6.3	CDS	79363195	79363467	79363195	ID=cds.MS.gene63458.t1;Parent=MS.gene63458.t1

Gene Sequence	>MS.gene63458 ATGAGAAATGAAGTTCCATTGTCCTTAGCCAAGACAGTGTTTTGCGGTGACTATAGAGATATACTTGAGAAAGTTGAAACTCCATGCACAATTATTCAAACCTCTAGTGACATGGTTGTTCCATATAGTGTAGCTCTTTACATGGAGAAGAAAATTAAAGGGAAAGTTACATTGGAGATAATTGACACTTTTGGACATTTTCCTCAACTAACTGCTCCACTTCAGCTGGTTGATGTGCTTAAGGGTGTTTTAGGGTTTGATCACTTCCCTTGA
Protein sequence	>MS.gene63458.t1 MRNEVPLSLAKTVFCGDYRDILEKVETPCTIIQTSSDMVVPYSVALYMEKKIKGKVTLEIIDTFGHFPQLTAPLQLVDVLKGVLGFDHFP