Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63573.t1 | XP_013459401.1 | 97 | 134 | 4 | 0 | 1 | 134 | 191 | 324 | 5.10E-65 | 256.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63573.t1 | A0A072UV00 | 97.0 | 134 | 4 | 0 | 1 | 134 | 191 | 324 | 3.7e-65 | 256.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene63573 | 0.829416 | 5.21E-55 | -1.69E-46 |
| MS.gene052628 | MS.gene63573 | 0.814067 | 1.86E-51 | -1.69E-46 |
| MS.gene052630 | MS.gene63573 | 0.801416 | 9.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63573.t1 | MTR_3g037110 | 97.015 | 134 | 4 | 0 | 1 | 134 | 191 | 324 | 1.11e-93 | 273 |
| MS.gene63573.t1 | MTR_4g109020 | 66.418 | 134 | 39 | 1 | 1 | 134 | 197 | 324 | 6.75e-55 | 174 |
| MS.gene63573.t1 | MTR_4g082350 | 34.021 | 97 | 61 | 1 | 1 | 97 | 237 | 330 | 8.32e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63573.t1 | AT5G61340 | 47.059 | 136 | 69 | 2 | 1 | 133 | 190 | 325 | 2.36e-36 | 127 |
| MS.gene63573.t1 | AT5G61340 | 47.059 | 136 | 69 | 2 | 1 | 133 | 223 | 358 | 3.86e-36 | 127 |
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGCATGTATTGTTCTA+AGG | 0.246591 | 3.2:-25405878 | MS.gene63573:CDS |
| AACATCAATGGCTTTGTTCT+TGG | 0.252405 | 3.2:-25405850 | MS.gene63573:CDS |
| TTGAAGCTTTGTTTCAATTC+CGG | 0.255839 | 3.2:-25405797 | MS.gene63573:CDS |
| ATAGAGGAGGACAGTTATTA+TGG | 0.314276 | 3.2:-25405585 | MS.gene63573:CDS |
| TGAAGCTTTGTTTCAATTCC+GGG | 0.319255 | 3.2:-25405796 | MS.gene63573:CDS |
| ACTTCCTGTTAATGTAGGTT+TGG | 0.385687 | 3.2:-25405826 | MS.gene63573:CDS |
| TTGGCACTTCCTGTTAATGT+AGG | 0.399443 | 3.2:-25405831 | MS.gene63573:CDS |
| GCAGCCAAACCTACATTAAC+AGG | 0.400186 | 3.2:+25405822 | None:intergenic |
| AACATGGCACTGACGCTATC+TGG | 0.405176 | 3.2:-25405933 | MS.gene63573:CDS |
| TACAAAAGCTGCAAAGTAAG+AGG | 0.421991 | 3.2:-25405654 | MS.gene63573:CDS |
| CTTGAAAATTCTTGATTCCC+AGG | 0.431044 | 3.2:+25405559 | None:intergenic |
| GGAGGGACATGGAGGTTATA+TGG | 0.435325 | 3.2:-25405907 | MS.gene63573:CDS |
| GAAAGCATGTATTGTTCTAA+GGG | 0.472724 | 3.2:-25405877 | MS.gene63573:CDS |
| GGACAGTTATTATGGTTACC+TGG | 0.477672 | 3.2:-25405577 | MS.gene63573:CDS |
| TTTCAATTCCGGGTCGTGAG+AGG | 0.505017 | 3.2:-25405786 | MS.gene63573:CDS |
| GAATTGAAACAAAGCTTCAA+TGG | 0.515494 | 3.2:+25405800 | None:intergenic |
| CTATCTGGTGTGGAGGGACA+TGG | 0.522310 | 3.2:-25405918 | MS.gene63573:CDS |
| TTTGAGGATTGAACTCATAG+AGG | 0.542567 | 3.2:-25405601 | MS.gene63573:CDS |
| TTCAATTCCGGGTCGTGAGA+GGG | 0.542858 | 3.2:-25405785 | MS.gene63573:CDS |
| GACAGTTATTATGGTTACCT+GGG | 0.552491 | 3.2:-25405576 | MS.gene63573:CDS |
| ATTACATTGATGAAAAGTCA+TGG | 0.569559 | 3.2:-25405761 | MS.gene63573:CDS |
| TGTAATACCCTCTCACGACC+CGG | 0.576169 | 3.2:+25405778 | None:intergenic |
| GAGGATTGAACTCATAGAGG+AGG | 0.588877 | 3.2:-25405598 | MS.gene63573:CDS |
| GGCACTGACGCTATCTGGTG+TGG | 0.597359 | 3.2:-25405928 | MS.gene63573:CDS |
| ACTGACGCTATCTGGTGTGG+AGG | 0.630820 | 3.2:-25405925 | MS.gene63573:CDS |
| CTGACGCTATCTGGTGTGGA+GGG | 0.659888 | 3.2:-25405924 | MS.gene63573:CDS |
| AAAGCATGTATTGTTCTAAG+GGG | 0.661511 | 3.2:-25405876 | MS.gene63573:CDS |
| AAGAGGATCATTGATTGACA+AGG | 0.665322 | 3.2:-25405637 | MS.gene63573:CDS |
| TCTGGTGTGGAGGGACATGG+AGG | 0.689970 | 3.2:-25405915 | MS.gene63573:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTACATTGATGAAAAGTCA+TGG | - | chr3.2:25405717-25405736 | MS.gene63573:CDS | 25.0% |
| GAATTGAAACAAAGCTTCAA+TGG | + | chr3.2:25405681-25405700 | None:intergenic | 30.0% | |
| ! | AAAGCATGTATTGTTCTAAG+GGG | - | chr3.2:25405602-25405621 | MS.gene63573:CDS | 30.0% |
| ! | GAAAGCATGTATTGTTCTAA+GGG | - | chr3.2:25405601-25405620 | MS.gene63573:CDS | 30.0% |
| ! | TGAAAGCATGTATTGTTCTA+AGG | - | chr3.2:25405600-25405619 | MS.gene63573:CDS | 30.0% |
| !! | TTGAAGCTTTGTTTCAATTC+CGG | - | chr3.2:25405681-25405700 | MS.gene63573:CDS | 30.0% |
| !!! | AGGAAGACAAACATTTTTTG+AGG | - | chr3.2:25405861-25405880 | MS.gene63573:CDS | 30.0% |
| ACTTCCTGTTAATGTAGGTT+TGG | - | chr3.2:25405652-25405671 | MS.gene63573:CDS | 35.0% | |
| ATAGAGGAGGACAGTTATTA+TGG | - | chr3.2:25405893-25405912 | MS.gene63573:CDS | 35.0% | |
| CTTGAAAATTCTTGATTCCC+AGG | + | chr3.2:25405922-25405941 | None:intergenic | 35.0% | |
| GACAGTTATTATGGTTACCT+GGG | - | chr3.2:25405902-25405921 | MS.gene63573:CDS | 35.0% | |
| TACAAAAGCTGCAAAGTAAG+AGG | - | chr3.2:25405824-25405843 | MS.gene63573:CDS | 35.0% | |
| TCTAAGGGGAAAAACATCAA+TGG | - | chr3.2:25405616-25405635 | MS.gene63573:CDS | 35.0% | |
| TTTGAGGATTGAACTCATAG+AGG | - | chr3.2:25405877-25405896 | MS.gene63573:CDS | 35.0% | |
| ! | AAGAGGATCATTGATTGACA+AGG | - | chr3.2:25405841-25405860 | MS.gene63573:CDS | 35.0% |
| ! | GTCATGGTTTTTCATAGCTT+TGG | - | chr3.2:25405733-25405752 | MS.gene63573:CDS | 35.0% |
| !! | AACATCAATGGCTTTGTTCT+TGG | - | chr3.2:25405628-25405647 | MS.gene63573:CDS | 35.0% |
| !! | TGAAGCTTTGTTTCAATTCC+GGG | - | chr3.2:25405682-25405701 | MS.gene63573:CDS | 35.0% |
| !!! | ATGGTTTTTCATAGCTTTGG+AGG | - | chr3.2:25405736-25405755 | MS.gene63573:CDS | 35.0% |
| !!! | TGGTTTTTCATAGCTTTGGA+GGG | - | chr3.2:25405737-25405756 | MS.gene63573:CDS | 35.0% |
| GGACAGTTATTATGGTTACC+TGG | - | chr3.2:25405901-25405920 | MS.gene63573:CDS | 40.0% | |
| TTGGCACTTCCTGTTAATGT+AGG | - | chr3.2:25405647-25405666 | MS.gene63573:CDS | 40.0% | |
| GAGGATTGAACTCATAGAGG+AGG | - | chr3.2:25405880-25405899 | MS.gene63573:CDS | 45.0% | |
| GCAGCCAAACCTACATTAAC+AGG | + | chr3.2:25405659-25405678 | None:intergenic | 45.0% | |
| GGAGGGACATGGAGGTTATA+TGG | - | chr3.2:25405571-25405590 | MS.gene63573:CDS | 50.0% | |
| TGTAATACCCTCTCACGACC+CGG | + | chr3.2:25405703-25405722 | None:intergenic | 50.0% | |
| TTCAATTCCGGGTCGTGAGA+GGG | - | chr3.2:25405693-25405712 | MS.gene63573:CDS | 50.0% | |
| TTTCAATTCCGGGTCGTGAG+AGG | - | chr3.2:25405692-25405711 | MS.gene63573:CDS | 50.0% | |
| ACTGACGCTATCTGGTGTGG+AGG | - | chr3.2:25405553-25405572 | MS.gene63573:CDS | 55.0% | |
| CTATCTGGTGTGGAGGGACA+TGG | - | chr3.2:25405560-25405579 | MS.gene63573:CDS | 55.0% | |
| CTGACGCTATCTGGTGTGGA+GGG | - | chr3.2:25405554-25405573 | MS.gene63573:CDS | 55.0% | |
| TCTGGTGTGGAGGGACATGG+AGG | - | chr3.2:25405563-25405582 | MS.gene63573:CDS | 60.0% | |
| !! | GGCACTGACGCTATCTGGTG+TGG | - | chr3.2:25405550-25405569 | MS.gene63573:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 25405548 | 25405952 | 25405548 | ID=MS.gene63573 |
| chr3.2 | mRNA | 25405548 | 25405952 | 25405548 | ID=MS.gene63573.t1;Parent=MS.gene63573 |
| chr3.2 | exon | 25405548 | 25405952 | 25405548 | ID=MS.gene63573.t1.exon1;Parent=MS.gene63573.t1 |
| chr3.2 | CDS | 25405548 | 25405952 | 25405548 | ID=cds.MS.gene63573.t1;Parent=MS.gene63573.t1 |
| Gene Sequence |
| Protein sequence |
