AlfalfaGEDB | MS.gene052628

M. sativa cultivar XinJiangDaYe / MS.gene052628

KEGG: K16292
GO: GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00112.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052628.t1	XP_013460973.1	97.9	287	6	0	1	287	76	362	6.10E-164	586.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052628.t1	P12412	84.3	287	45	0	1	287	76	362	2.3e-143	509.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052628.t1	G8A2F7	97.9	287	6	0	1	287	76	362	4.4e-164	586.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049227	MS.gene052628	0.815189	1.05E-51	-1.69E-46
MS.gene049807	MS.gene052628	0.806732	7.13E-50	-1.69E-46
MS.gene051279	MS.gene052628	0.817004	4.13E-52	-1.69E-46
MS.gene052239	MS.gene052628	-0.814079	1.85E-51	-1.69E-46
MS.gene052628	MS.gene052630	0.934548	3.40E-96	-1.69E-46
MS.gene052628	MS.gene05894	0.821253	4.45E-53	-1.69E-46
MS.gene052628	MS.gene061100	0.811154	8.07E-51	-1.69E-46
MS.gene052628	MS.gene061707	0.817458	3.26E-52	-1.69E-46
MS.gene052628	MS.gene062042	0.802733	4.88E-49	-1.69E-46
MS.gene052628	MS.gene06341	0.812654	3.80E-51	-1.69E-46
MS.gene052628	MS.gene064518	0.803889	2.81E-49	-1.69E-46
MS.gene052628	MS.gene069852	0.801046	1.08E-48	-1.69E-46
MS.gene052628	MS.gene074317	0.826509	2.61E-54	-1.69E-46
MS.gene052628	MS.gene07723	0.80552	1.28E-49	-1.69E-46
MS.gene052628	MS.gene24050	0.807649	4.56E-50	-1.69E-46
MS.gene052628	MS.gene25981	-0.806191	9.27E-50	-1.69E-46
MS.gene052628	MS.gene27206	0.833418	5.40E-56	-1.69E-46
MS.gene052628	MS.gene27646	-0.809376	1.95E-50	-1.69E-46
MS.gene052628	MS.gene29082	0.987764	1.89E-171	-1.69E-46
MS.gene052628	MS.gene31222	0.854522	1.16E-61	-1.69E-46
MS.gene052628	MS.gene31803	0.800351	1.50E-48	-1.69E-46
MS.gene052628	MS.gene33059	0.812928	3.31E-51	-1.69E-46
MS.gene052628	MS.gene34164	0.829812	4.17E-55	-1.69E-46
MS.gene052628	MS.gene34266	0.839058	1.99E-57	-1.69E-46
MS.gene052628	MS.gene35375	0.814563	1.45E-51	-1.69E-46
MS.gene052628	MS.gene35858	0.80392	2.77E-49	-1.69E-46
MS.gene052628	MS.gene36334	0.807874	4.08E-50	-1.69E-46
MS.gene052628	MS.gene37106	0.818141	2.29E-52	-1.69E-46
MS.gene052628	MS.gene42534	0.802791	4.74E-49	-1.69E-46
MS.gene052628	MS.gene47768	0.831571	1.55E-55	-1.69E-46
MS.gene052628	MS.gene51877	0.831188	1.92E-55	-1.69E-46
MS.gene052628	MS.gene58248	0.812937	3.30E-51	-1.69E-46
MS.gene052628	MS.gene63573	0.814067	1.86E-51	-1.69E-46
MS.gene052628	MS.gene67631	0.810578	1.07E-50	-1.69E-46
MS.gene052628	MS.gene68663	0.830665	2.58E-55	-1.69E-46
MS.gene052628	MS.gene71236	0.801619	8.27E-49	-1.69E-46
MS.gene052628	MS.gene72487	0.802272	6.07E-49	-1.69E-46
MS.gene052628	MS.gene74758	0.806999	6.26E-50	-1.69E-46
MS.gene052628	MS.gene76518	0.816301	5.94E-52	-1.69E-46
MS.gene052628	MS.gene76890	0.824982	6.01E-54	-1.69E-46
MS.gene052628	MS.gene78715	0.87887	1.98E-69	-1.69E-46
MS.gene052628	MS.gene82912	0.820391	7.03E-53	-1.69E-46
MS.gene052628	MS.gene85133	0.815982	6.99E-52	-1.69E-46
MS.gene052628	MS.gene90591	0.805719	1.17E-49	-1.69E-46
MS.gene052628	MS.gene97017	0.807232	5.59E-50	-1.69E-46
MS.gene052628	MS.gene99427	0.819771	9.75E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene045501	MS.gene052628	PPI
MS.gene031581	MS.gene052628	PPI
MS.gene043473	MS.gene052628	PPI
MS.gene026873	MS.gene052628	PPI
MS.gene006646	MS.gene052628	PPI
MS.gene003680	MS.gene052628	PPI
MS.gene012206	MS.gene052628	PPI
MS.gene26669	MS.gene052628	PPI
MS.gene028850	MS.gene052628	PPI
MS.gene052628	MS.gene006680	PPI
MS.gene069978	MS.gene052628	PPI
MS.gene50738	MS.gene052628	PPI
MS.gene31410	MS.gene052628	PPI
MS.gene006665	MS.gene052628	PPI
MS.gene031580	MS.gene052628	PPI
MS.gene51064	MS.gene052628	PPI
MS.gene026876	MS.gene052628	PPI
MS.gene014304	MS.gene052628	PPI
MS.gene045525	MS.gene052628	PPI
MS.gene24818	MS.gene052628	PPI
MS.gene23321	MS.gene052628	PPI
MS.gene69195	MS.gene052628	PPI
MS.gene052628	MS.gene006720	PPI
MS.gene63396	MS.gene052628	PPI
MS.gene058156	MS.gene052628	PPI
MS.gene67468	MS.gene052628	PPI
MS.gene031638	MS.gene052628	PPI
MS.gene73838	MS.gene052628	PPI
MS.gene045458	MS.gene052628	PPI
MS.gene064873	MS.gene052628	PPI
MS.gene63719	MS.gene052628	PPI
MS.gene031619	MS.gene052628	PPI
MS.gene052628	MS.gene26670	PPI
MS.gene052628	MS.gene005934	PPI
MS.gene052628	MS.gene065787	PPI
MS.gene73835	MS.gene052628	PPI
MS.gene87639	MS.gene052628	PPI
MS.gene031635	MS.gene052628	PPI
MS.gene26853	MS.gene052628	PPI
MS.gene027420	MS.gene052628	PPI
MS.gene052628	MS.gene64795	PPI
MS.gene73834	MS.gene052628	PPI
MS.gene005933	MS.gene052628	PPI
MS.gene006682	MS.gene052628	PPI
MS.gene73815	MS.gene052628	PPI
MS.gene026874	MS.gene052628	PPI
MS.gene59493	MS.gene052628	PPI
MS.gene045455	MS.gene052628	PPI
MS.gene052628	MS.gene045503	PPI
MS.gene045459	MS.gene052628	PPI
MS.gene045526	MS.gene052628	PPI
MS.gene66833	MS.gene052628	PPI
MS.gene033151	MS.gene052628	PPI
MS.gene09529	MS.gene052628	PPI
MS.gene25255	MS.gene052628	PPI
MS.gene055760	MS.gene052628	PPI
MS.gene045461	MS.gene052628	PPI
MS.gene052628	MS.gene045522	PPI
MS.gene031621	MS.gene052628	PPI
MS.gene73817	MS.gene052628	PPI
MS.gene065785	MS.gene052628	PPI
MS.gene95543	MS.gene052628	PPI
MS.gene26670	MS.gene052628	PPI
MS.gene003677	MS.gene052628	PPI
MS.gene060218	MS.gene052628	PPI
MS.gene73840	MS.gene052628	PPI
MS.gene052628	MS.gene045520	PPI
MS.gene34090	MS.gene052628	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052628.t1	MTR_3g075390	97.909	287	6	0	1	287	76	362	0.0	596
MS.gene052628.t1	MTR_4g104370	77.352	287	63	2	1	287	76	360	1.29e-168	471
MS.gene052628.t1	MTR_4g080700	60.821	268	96	5	2	268	79	338	2.33e-115	335
MS.gene052628.t1	MTR_4g080730	60.448	268	97	5	2	268	79	338	2.41e-115	335
MS.gene052628.t1	MTR_4g107930	59.191	272	98	3	2	268	80	343	7.12e-115	334
MS.gene052628.t1	MTR_4g047610	61.567	268	98	4	2	268	78	341	7.77e-115	334
MS.gene052628.t1	MTR_5g022560	59.779	271	98	4	2	268	79	342	1.67e-114	333
MS.gene052628.t1	MTR_4g079770	61.194	268	98	4	2	268	81	343	3.18e-114	333
MS.gene052628.t1	MTR_4g079470	59.926	272	95	4	2	268	81	343	5.08e-114	332
MS.gene052628.t1	MTR_1g107550	60.674	267	102	3	5	270	92	356	1.00e-112	333
MS.gene052628.t1	MTR_4g079800	58.824	272	98	4	2	268	81	343	1.11e-112	329
MS.gene052628.t1	MTR_4g094918	57.090	268	112	3	5	270	76	342	2.89e-109	321
MS.gene052628.t1	MTR_1g018840	56.777	273	115	3	1	271	92	363	1.03e-108	323
MS.gene052628.t1	MTR_1g018840	56.777	273	115	3	1	271	92	363	1.89e-108	323
MS.gene052628.t1	MTR_2g075830	60.741	270	95	3	2	268	78	339	2.86e-108	317
MS.gene052628.t1	MTR_4g079440	59.551	267	97	5	5	269	87	344	8.14e-108	317
MS.gene052628.t1	MTR_3g102220	57.196	271	113	3	2	270	93	362	1.57e-107	320
MS.gene052628.t1	MTR_2g090890	57.090	268	105	3	5	271	80	338	2.50e-107	315
MS.gene052628.t1	MTR_3g116080	57.895	266	107	3	5	270	88	348	3.21e-107	315
MS.gene052628.t1	MTR_4g080360	54.478	268	120	1	2	269	76	341	8.48e-107	314
MS.gene052628.t1	MTR_2g083930	52.593	270	126	2	2	269	74	343	8.97e-99	293
MS.gene052628.t1	MTR_7g079230	52.239	268	116	4	2	268	78	334	1.40e-92	278
MS.gene052628.t1	MTR_7g078600	50.549	273	120	5	2	272	81	340	2.13e-92	277
MS.gene052628.t1	MTR_3g112430	51.866	268	128	1	5	272	78	344	2.43e-92	280
MS.gene052628.t1	MTR_2g016180	48.148	270	128	7	5	270	95	356	1.69e-78	246
MS.gene052628.t1	MTR_2g016270	47.778	270	114	7	5	270	95	341	7.00e-78	244
MS.gene052628.t1	MTR_2g015850	46.642	268	138	4	2	269	101	363	9.23e-77	238
MS.gene052628.t1	MTR_4g125300	45.387	271	128	9	5	269	92	348	3.27e-66	210
MS.gene052628.t1	MTR_8g086470	46.886	273	127	10	5	270	96	357	1.00e-64	207
MS.gene052628.t1	MTR_4g065077	46.886	273	127	10	5	270	96	357	1.39e-64	207
MS.gene052628.t1	MTR_2g015920	41.812	287	152	9	5	286	97	373	4.48e-64	206
MS.gene052628.t1	MTR_2g067900	42.909	275	141	10	5	269	58	326	3.24e-58	189
MS.gene052628.t1	MTR_1g023810	42.731	227	122	6	44	267	40	261	6.10e-57	184
MS.gene052628.t1	MTR_2g015930	41.667	276	138	10	5	270	97	359	1.05e-56	186
MS.gene052628.t1	MTR_1g023210	39.711	277	139	9	1	261	88	352	4.86e-56	184
MS.gene052628.t1	MTR_4g071060	38.267	277	142	8	1	261	85	348	6.33e-54	179
MS.gene052628.t1	MTR_2g064475	38.816	304	138	11	2	267	55	348	8.40e-53	176
MS.gene052628.t1	MTR_7g078630	48.815	211	88	6	32	229	747	950	1.96e-52	184
MS.gene052628.t1	MTR_2g067560	40.727	275	138	11	5	263	58	323	4.06e-52	183
MS.gene052628.t1	MTR_1g094135	36.765	272	139	9	9	261	101	358	6.23e-49	167
MS.gene052628.t1	MTR_2g066980	33.922	283	133	9	2	268	73	317	3.42e-46	158
MS.gene052628.t1	MTR_7g078580	64.646	99	34	1	171	269	4	101	9.59e-44	145
MS.gene052628.t1	MTR_2g015840	32.836	268	110	6	2	268	72	270	1.07e-38	137
MS.gene052628.t1	MTR_7g079130	64.835	91	32	0	179	269	7	97	1.46e-38	131
MS.gene052628.t1	MTR_4g072790	34.470	264	134	7	1	261	85	312	5.34e-38	137
MS.gene052628.t1	MTR_2g047960	77.215	79	18	0	62	140	7	85	2.00e-36	125
MS.gene052628.t1	MTR_4g073660	33.829	269	133	10	1	261	84	315	3.67e-35	129
MS.gene052628.t1	MTR_4g072340	33.829	269	133	10	1	261	85	316	4.86e-35	129
MS.gene052628.t1	MTR_1g025450	41.954	174	77	7	52	221	54	207	8.28e-33	126
MS.gene052628.t1	MTR_2g016380	45.902	122	59	4	153	270	18	136	7.84e-29	110
MS.gene052628.t1	MTR_7g111060	27.536	276	164	11	11	261	66	330	2.47e-24	100
MS.gene052628.t1	MTR_7g111050	30.738	244	137	10	55	275	106	340	4.74e-23	97.4
MS.gene052628.t1	MTR_3g029560	32.381	210	117	7	64	269	49	237	3.88e-21	90.1
MS.gene052628.t1	MTR_7g079260	56.944	72	15	1	195	266	7	62	1.12e-20	85.1
MS.gene052628.t1	MTR_8g087510	41.538	130	70	5	94	221	23	148	4.20e-19	82.8
MS.gene052628.t1	MTR_7g078310	37.063	143	76	4	3	142	113	244	1.56e-18	83.6
MS.gene052628.t1	MTR_7g111050	29.798	198	116	7	55	231	101	296	2.96e-15	74.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052628.t1	AT5G50260	73.868	287	74	1	2	287	75	361	7.90e-164	459
MS.gene052628.t1	AT3G48340	69.444	288	85	2	2	287	75	361	2.03e-149	423
MS.gene052628.t1	AT3G48350	67.596	287	91	2	2	287	75	360	6.35e-145	411
MS.gene052628.t1	AT1G20850	58.582	268	108	2	3	270	90	354	1.15e-110	324
MS.gene052628.t1	AT5G45890	55.147	272	110	4	3	268	79	344	2.26e-109	320
MS.gene052628.t1	AT4G35350	59.398	266	104	2	5	270	92	353	8.45e-109	319
MS.gene052628.t1	AT5G43060	59.023	266	103	3	5	270	95	354	1.01e-108	323
MS.gene052628.t1	AT1G47128	56.554	267	111	2	5	271	93	354	1.83e-108	322
MS.gene052628.t1	AT3G19390	54.909	275	118	3	2	275	82	351	1.11e-106	317
MS.gene052628.t1	AT4G36880	56.364	275	116	4	5	277	96	368	1.50e-105	312
MS.gene052628.t1	AT3G48350	68.966	203	62	1	2	203	75	277	4.02e-102	300
MS.gene052628.t1	AT3G19400	53.004	283	125	5	2	281	83	360	5.83e-100	297
MS.gene052628.t1	AT4G11310	54.579	273	112	5	5	272	90	355	1.96e-97	291
MS.gene052628.t1	AT1G06260	54.380	274	111	5	1	270	80	343	5.11e-97	289
MS.gene052628.t1	AT4G11320	54.212	273	113	5	5	272	97	362	1.06e-95	286
MS.gene052628.t1	AT4G11320	54.212	273	113	5	5	272	97	362	1.06e-95	286
MS.gene052628.t1	AT1G09850	52.632	266	121	2	5	270	74	334	1.21e-94	286
MS.gene052628.t1	AT3G49340	49.259	270	132	3	2	269	74	340	3.85e-92	276
MS.gene052628.t1	AT2G27420	50.000	272	128	3	5	269	77	347	6.30e-91	274
MS.gene052628.t1	AT4G23520	49.451	273	130	5	5	275	89	355	1.29e-90	273
MS.gene052628.t1	AT2G34080	49.265	272	129	4	2	269	78	344	1.07e-86	263
MS.gene052628.t1	AT1G29090	48.746	279	122	6	2	269	86	354	2.36e-83	254
MS.gene052628.t1	AT1G29080	45.926	270	142	3	2	269	78	345	3.54e-82	251
MS.gene052628.t1	AT3G43960	47.183	284	138	8	2	279	80	357	1.76e-80	248
MS.gene052628.t1	AT4G35350	58.794	199	78	2	5	203	92	286	5.35e-75	231
MS.gene052628.t1	AT1G29110	43.750	272	134	5	2	269	77	333	7.38e-71	222
MS.gene052628.t1	AT3G19400	53.000	200	87	4	2	198	83	278	1.30e-64	204
MS.gene052628.t1	AT5G60360	43.173	271	134	9	5	269	100	356	2.46e-62	201
MS.gene052628.t1	AT3G45310	42.125	273	134	10	5	269	100	356	1.54e-60	196
MS.gene052628.t1	AT5G60360	42.804	271	134	10	5	269	100	355	1.00e-59	194
MS.gene052628.t1	AT3G45310	41.328	271	138	10	5	269	100	355	5.43e-58	189
MS.gene052628.t1	AT5G60360	43.028	251	127	8	5	249	100	340	1.86e-57	188
MS.gene052628.t1	AT2G21430	37.906	277	144	8	1	261	85	349	2.81e-54	180
MS.gene052628.t1	AT4G39090	38.290	269	138	8	9	261	96	352	2.39e-52	175
MS.gene052628.t1	AT3G54940	39.350	277	142	9	1	261	88	354	4.92e-52	174
MS.gene052628.t1	AT4G16190	39.033	269	138	9	9	261	100	358	9.92e-50	169
MS.gene052628.t1	AT3G54940	39.896	193	104	4	1	185	88	276	1.08e-35	129
MS.gene052628.t1	AT1G02305	29.373	303	177	11	10	287	68	358	4.92e-27	108
MS.gene052628.t1	AT4G01610	29.352	293	166	13	10	275	65	343	1.85e-23	98.6
MS.gene052628.t1	AT4G01610	28.669	293	168	13	10	275	65	343	1.67e-21	93.2
MS.gene052628.t1	AT1G02300	31.381	239	132	10	72	287	146	375	2.50e-20	90.1
MS.gene052628.t1	AT2G22160	51.351	74	32	1	3	76	33	102	4.67e-16	72.8

Find 75 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAACCTATCTGTCTTTATT+TGG	0.189761	3.1:+62270483	None:intergenic
CCGTTGATGGAACTAATTAT+TGG	0.225728	3.1:-62269833	MS.gene052628:CDS
TTTGAGTACATTAAGCAAAA+AGG	0.295259	3.1:-62270380	MS.gene052628:CDS
ATCAGATCCTCCGGCATCTA+TGG	0.300046	3.1:+62270099	None:intergenic
ACATGACCAACCATGAATTT+AGG	0.309976	3.1:-62271065	MS.gene052628:CDS
GAACTCATGGGGAGCGGAAT+GGG	0.328427	3.1:-62269802	MS.gene052628:CDS
AAGCTCCTGCTTCTGTTGAT+TGG	0.348923	3.1:-62270951	MS.gene052628:CDS
CTATTACAGGGAGTATTTAC+TGG	0.367645	3.1:-62269909	MS.gene052628:intron
GCATCTATGGCTACGGATAC+AGG	0.385812	3.1:+62270112	None:intergenic
TCAACTGTTGTAGCTGTTGA+AGG	0.388321	3.1:-62270512	MS.gene052628:CDS
TTGTTAAGTTTCAACTTGTA+AGG	0.389898	3.1:+62271096	None:intergenic
AGGATGCAATGGTGGGCTAA+TGG	0.392108	3.1:-62270411	MS.gene052628:CDS
GATCCATCATTTGCTGTGTA+AGG	0.397020	3.1:+62270332	None:intergenic
GTCCCAGCTGTATCAATTGA+TGG	0.409945	3.1:-62270193	MS.gene052628:CDS
CACTTCCACGTGGCGTGCCT+CGG	0.411657	3.1:+62271001	None:intergenic
ATCAACAGAAGCAGGAGCTT+TGG	0.419799	3.1:+62270954	None:intergenic
TGACCATCAATTGATACAGC+TGG	0.435646	3.1:+62270190	None:intergenic
GGTGTAGCAATAGTAGGATA+TGG	0.443941	3.1:-62269861	MS.gene052628:CDS
TGATGGTCATGAGACTGTTC+CGG	0.449890	3.1:-62270176	MS.gene052628:CDS
GGATATGGAACAACCGTTGA+TGG	0.454887	3.1:-62269846	MS.gene052628:CDS
CGAGGCACGCCACGTGGAAG+TGG	0.460548	3.1:-62271000	MS.gene052628:CDS
TCATCCTTGGTAGATGATAA+AGG	0.462977	3.1:+62269666	None:intergenic
TAAACCAAATAAAGACAGAT+AGG	0.464117	3.1:-62270487	MS.gene052628:CDS
TTCCTCCAATCAACAGAAGC+AGG	0.475830	3.1:+62270946	None:intergenic
CATAGGTAGTCCTAAATTCA+TGG	0.476124	3.1:+62271055	None:intergenic
CTCCTGCTTCTGTTGATTGG+AGG	0.481655	3.1:-62270948	MS.gene052628:CDS
ATTGGATTGTGAGGAACTCA+TGG	0.493389	3.1:-62269815	MS.gene052628:CDS
AATCATGGTGTAGCAATAGT+AGG	0.495226	3.1:-62269867	MS.gene052628:CDS
GTCACTGAGGTCAAGGATCA+AGG	0.495703	3.1:-62270913	MS.gene052628:CDS
TCTAACAAGGAAGGACTTTG+TGG	0.498260	3.1:-62269744	MS.gene052628:CDS
GTAGATGATAAAGGTCCTGC+TGG	0.507201	3.1:+62269675	None:intergenic
AAACATTCAAAGCTCATCCT+TGG	0.508057	3.1:+62269653	None:intergenic
TACCCTTACACAGCAAATGA+TGG	0.512248	3.1:-62270335	MS.gene052628:CDS
GAATTTAGGACTACCTATGC+TGG	0.513702	3.1:-62271051	MS.gene052628:CDS
GGAACTCATGGGGAGCGGAA+TGG	0.524402	3.1:-62269803	MS.gene052628:CDS
AGGACTTTGTGGCATAGCTA+TGG	0.526821	3.1:-62269733	MS.gene052628:CDS
TGTGACAATCAAGAAAATCA+AGG	0.527505	3.1:-62270431	MS.gene052628:CDS
GAAAATCAAGGATGCAATGG+TGG	0.535112	3.1:-62270419	MS.gene052628:CDS
TCCTCCGGCATCTATGGCTA+CGG	0.543132	3.1:+62270105	None:intergenic
GACTACCTATGCTGGATCAA+AGG	0.546319	3.1:-62271043	MS.gene052628:CDS
CAAGAAAATCAAGGATGCAA+TGG	0.548537	3.1:-62270422	MS.gene052628:CDS
AAACTGGAAATCAGATCCTC+CGG	0.552551	3.1:+62270090	None:intergenic
TCTGTTGATTGGAGGAAGAA+AGG	0.553167	3.1:-62270940	MS.gene052628:CDS
GGCTACGGATACAGGTTGGT+TGG	0.553342	3.1:+62270120	None:intergenic
AGGTGCTGTCACTGAGGTCA+AGG	0.558185	3.1:-62270920	MS.gene052628:CDS
ATCCATCATTTGCTGTGTAA+GGG	0.560080	3.1:+62270333	None:intergenic
TGTGGTAAAGAGCTAAATCA+TGG	0.564153	3.1:-62269882	MS.gene052628:CDS
CCAATAATTAGTTCCATCAA+CGG	0.573198	3.1:+62269833	None:intergenic
TTGGATTGTGAGGAACTCAT+GGG	0.574375	3.1:-62269814	MS.gene052628:CDS
AACTCATGGGGAGCGGAATG+GGG	0.577276	3.1:-62269801	MS.gene052628:CDS
GACCATCAATTGATACAGCT+GGG	0.584179	3.1:+62270191	None:intergenic
AAAGCATCTTCATCGTTAGC+CGG	0.584931	3.1:+62270157	None:intergenic
AAATTCATGGTTGGTCATGT+CGG	0.587621	3.1:+62271068	None:intergenic
GGAGCGGAATGGGGTGAACA+AGG	0.588520	3.1:-62269792	MS.gene052628:CDS
ATTAACCTTTGATCCAGCAT+AGG	0.590030	3.1:+62271038	None:intergenic
GGAGTATTTACTGGCGATTG+TGG	0.593617	3.1:-62269900	MS.gene052628:CDS
TGTGAGGAACTCATGGGGAG+CGG	0.601438	3.1:-62269808	MS.gene052628:CDS
ATGTTCCGAGGCACGCCACG+TGG	0.610728	3.1:-62271006	MS.gene052628:CDS
GCGTGCCTCGGAACATTCTG+TGG	0.611727	3.1:+62271013	None:intergenic
AGGAATGTATCTAACAAGGA+AGG	0.612463	3.1:-62269753	MS.gene052628:CDS
GAACTAATTATTGGATTGTG+AGG	0.613342	3.1:-62269824	MS.gene052628:CDS
GGTAGTCCTAAATTCATGGT+TGG	0.613532	3.1:+62271059	None:intergenic
AAAATCAAGGATGCAATGGT+GGG	0.617377	3.1:-62270418	MS.gene052628:CDS
ATAAGCTCTTGTTCAGACAA+AGG	0.634536	3.1:+62270458	None:intergenic
AAGGTTACATCAGAATGCAA+AGG	0.641547	3.1:-62269773	MS.gene052628:CDS
GCAAAGGAATGTATCTAACA+AGG	0.642601	3.1:-62269757	MS.gene052628:CDS
CTATGGCTACGGATACAGGT+TGG	0.643007	3.1:+62270116	None:intergenic
GTATCCGTAGCCATAGATGC+CGG	0.655334	3.1:-62270109	MS.gene052628:CDS
AGGACCTTTATCATCTACCA+AGG	0.670444	3.1:-62269670	MS.gene052628:CDS
AATCACCACAGAATGTTCCG+AGG	0.693791	3.1:-62271018	MS.gene052628:CDS
TCCGTAGCCATAGATGCCGG+AGG	0.697215	3.1:-62270106	MS.gene052628:CDS
GTCAAGGATCAAGGCCAATG+TGG	0.699707	3.1:-62270904	MS.gene052628:intron
TGGATTGTGAGGAACTCATG+GGG	0.709984	3.1:-62269813	MS.gene052628:CDS
GAAGAAAGGTGCTGTCACTG+AGG	0.722701	3.1:-62270926	MS.gene052628:CDS
ATGAATGTTCCACTTCCACG+TGG	0.756623	3.1:+62270991	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTACTTTTTTATTTTGA+AGG	+	chr3.1:62270763-62270782	None:intergenic	10.0%
!!	AGTGAATTGAATTTATAAAT+AGG	+	chr3.1:62270038-62270057	None:intergenic	15.0%
!!	TAAAAAAGTAAAAAAAGAGA+TGG	-	chr3.1:62270769-62270788	MS.gene052628:intron	15.0%
!!	TAGTATAACTATTTGAAAAT+AGG	+	chr3.1:62270102-62270121	None:intergenic	15.0%
!!!	ATGAACAATTTTTGAATCTT+AGG	+	chr3.1:62270538-62270557	None:intergenic	20.0%
!	TAAACCAAATAAAGACAGAT+AGG	-	chr3.1:62270277-62270296	MS.gene052628:intron	25.0%
!	TATAATTGACATGCAACTTT+AGG	-	chr3.1:62270510-62270529	MS.gene052628:CDS	25.0%
!	TTGTTAAGTTTCAACTTGTA+AGG	+	chr3.1:62269671-62269690	None:intergenic	25.0%
!	TTTGAGTACATTAAGCAAAA+AGG	-	chr3.1:62270384-62270403	MS.gene052628:CDS	25.0%
!!	ATTTTGTATACACCTATTAC+AGG	-	chr3.1:62270842-62270861	MS.gene052628:intron	25.0%
!!	TATTGACTACATAAATGCAT+AGG	-	chr3.1:62270058-62270077	MS.gene052628:intron	25.0%
!!	TTGTGATTAATGTTTTGTAC+AGG	-	chr3.1:62270213-62270232	MS.gene052628:intron	25.0%
!!	TTTTGTATACACCTATTACA+GGG	-	chr3.1:62270843-62270862	MS.gene052628:intron	25.0%
!!!	CTTTTTTTAACTTACCACAT+TGG	+	chr3.1:62269877-62269896	None:intergenic	25.0%
	AAAGCTATAGTGCATAATAG+TGG	+	chr3.1:62270178-62270197	None:intergenic	30.0%
	CCAATAATTAGTTCCATCAA+CGG	+	chr3.1:62270934-62270953	None:intergenic	30.0%
	CTAACCTATCTGTCTTTATT+TGG	+	chr3.1:62270284-62270303	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	-	chr3.1:62270940-62270959	MS.gene052628:CDS	30.0%
	TCAAAAATTGTTCATGTTGC+AGG	-	chr3.1:62270543-62270562	MS.gene052628:intron	30.0%
	TGTGACAATCAAGAAAATCA+AGG	-	chr3.1:62270333-62270352	MS.gene052628:CDS	30.0%
!	ATCTGATTTCCAGTTTTACT+CGG	-	chr3.1:62270681-62270700	MS.gene052628:intron	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	+	chr3.1:62271067-62271086	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	+	chr3.1:62271068-62271087	None:intergenic	30.0%
	AAAATCAAGGATGCAATGGT+GGG	-	chr3.1:62270346-62270365	MS.gene052628:CDS	35.0%
	AAGGTTACATCAGAATGCAA+AGG	-	chr3.1:62270991-62271010	MS.gene052628:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	-	chr3.1:62270897-62270916	MS.gene052628:intron	35.0%
	ACATGACCAACCATGAATTT+AGG	-	chr3.1:62269699-62269718	MS.gene052628:CDS	35.0%
	AGGAATGTATCTAACAAGGA+AGG	-	chr3.1:62271011-62271030	MS.gene052628:CDS	35.0%
	ATAAGCTCTTGTTCAGACAA+AGG	+	chr3.1:62270309-62270328	None:intergenic	35.0%
	ATTAACCTTTGATCCAGCAT+AGG	+	chr3.1:62269729-62269748	None:intergenic	35.0%
	ATTCTCTTGTGTCAATAGAG+AGG	+	chr3.1:62270727-62270746	None:intergenic	35.0%
	CAAGAAAATCAAGGATGCAA+TGG	-	chr3.1:62270342-62270361	MS.gene052628:CDS	35.0%
	CAGTAAATACTCCCTGTAAT+AGG	+	chr3.1:62270857-62270876	None:intergenic	35.0%
	CATAGGTAGTCCTAAATTCA+TGG	+	chr3.1:62269712-62269731	None:intergenic	35.0%
	GAGTACATTAAGCAAAAAGG+TGG	-	chr3.1:62270387-62270406	MS.gene052628:CDS	35.0%
	GCAAAGGAATGTATCTAACA+AGG	-	chr3.1:62271007-62271026	MS.gene052628:CDS	35.0%
	GTTGCAAACCTGAAACTTAA+AGG	-	chr3.1:62269994-62270013	MS.gene052628:intron	35.0%
	TCATCCTTGGTAGATGATAA+AGG	+	chr3.1:62271101-62271120	None:intergenic	35.0%
	TGTGGTAAAGAGCTAAATCA+TGG	-	chr3.1:62270882-62270901	MS.gene052628:intron	35.0%
!	AAATTCATGGTTGGTCATGT+CGG	+	chr3.1:62269699-62269718	None:intergenic	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	-	chr3.1:62270931-62270950	MS.gene052628:CDS	35.0%
!	TTTTTACCTCCGAGTAAAAC+TGG	+	chr3.1:62270693-62270712	None:intergenic	35.0%
!!	ATCCATCATTTGCTGTGTAA+GGG	+	chr3.1:62270434-62270453	None:intergenic	35.0%
!!	CTATTACAGGGAGTATTTAC+TGG	-	chr3.1:62270855-62270874	MS.gene052628:intron	35.0%
	AAACTGGAAATCAGATCCTC+CGG	+	chr3.1:62270677-62270696	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	-	chr3.1:62271094-62271113	MS.gene052628:CDS	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	-	chr3.1:62270949-62270968	MS.gene052628:CDS	40.0%
	GAAAATCAAGGATGCAATGG+TGG	-	chr3.1:62270345-62270364	MS.gene052628:CDS	40.0%
	GACACCATCTGATCAAAGAT+CGG	-	chr3.1:62269948-62269967	MS.gene052628:intron	40.0%
	GACCATCAATTGATACAGCT+GGG	+	chr3.1:62270576-62270595	None:intergenic	40.0%
	GGTAGTCCTAAATTCATGGT+TGG	+	chr3.1:62269708-62269727	None:intergenic	40.0%
	GGTGTAGCAATAGTAGGATA+TGG	-	chr3.1:62270903-62270922	MS.gene052628:intron	40.0%
	TACCCTTACACAGCAAATGA+TGG	-	chr3.1:62270429-62270448	MS.gene052628:CDS	40.0%
	TCAACTGTTGTAGCTGTTGA+AGG	-	chr3.1:62270252-62270271	MS.gene052628:intron	40.0%
	TCATCCGATCTTTGATCAGA+TGG	+	chr3.1:62269955-62269974	None:intergenic	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	-	chr3.1:62271020-62271039	MS.gene052628:CDS	40.0%
	TGACCATCAATTGATACAGC+TGG	+	chr3.1:62270577-62270596	None:intergenic	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	-	chr3.1:62270950-62270969	MS.gene052628:CDS	40.0%
!	AAAGCATCTTCATCGTTAGC+CGG	+	chr3.1:62270610-62270629	None:intergenic	40.0%
!	GAATTTAGGACTACCTATGC+TGG	-	chr3.1:62269713-62269732	MS.gene052628:CDS	40.0%
!	GTTTTGTACAGGTAGTTGCT+GGG	-	chr3.1:62270224-62270243	MS.gene052628:intron	40.0%
!	TAAAGGTCCTGCTGGATTTT+TGG	+	chr3.1:62271084-62271103	None:intergenic	40.0%
!	TCTGTTGATTGGAGGAAGAA+AGG	-	chr3.1:62269824-62269843	MS.gene052628:CDS	40.0%
!	TGATTTCCAGTTTTACTCGG+AGG	-	chr3.1:62270684-62270703	MS.gene052628:intron	40.0%
!	TGTTTTGTACAGGTAGTTGC+TGG	-	chr3.1:62270223-62270242	MS.gene052628:intron	40.0%
!!	GATCCATCATTTGCTGTGTA+AGG	+	chr3.1:62270435-62270454	None:intergenic	40.0%
	AACTCCTCCAAAAATCCAGC+AGG	-	chr3.1:62271074-62271093	MS.gene052628:CDS	45.0%
	AAGCTCCTGCTTCTGTTGAT+TGG	-	chr3.1:62269813-62269832	MS.gene052628:CDS	45.0%
	AATCACCACAGAATGTTCCG+AGG	-	chr3.1:62269746-62269765	MS.gene052628:CDS	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	-	chr3.1:62271031-62271050	MS.gene052628:CDS	45.0%
	ATCAACAGAAGCAGGAGCTT+TGG	+	chr3.1:62269813-62269832	None:intergenic	45.0%
	ATGAATGTTCCACTTCCACG+TGG	+	chr3.1:62269776-62269795	None:intergenic	45.0%
	CCTGAAACTTAAAGGACTCG+TGG	-	chr3.1:62270002-62270021	MS.gene052628:intron	45.0%
	GACTACCTATGCTGGATCAA+AGG	-	chr3.1:62269721-62269740	MS.gene052628:CDS	45.0%
	GGATATGGAACAACCGTTGA+TGG	-	chr3.1:62270918-62270937	MS.gene052628:CDS	45.0%
	GTAGATGATAAAGGTCCTGC+TGG	+	chr3.1:62271092-62271111	None:intergenic	45.0%
	GTCCCAGCTGTATCAATTGA+TGG	-	chr3.1:62270571-62270590	MS.gene052628:intron	45.0%
	TGATGGTCATGAGACTGTTC+CGG	-	chr3.1:62270588-62270607	MS.gene052628:intron	45.0%
	TGGATTGTGAGGAACTCATG+GGG	-	chr3.1:62270951-62270970	MS.gene052628:CDS	45.0%
	TTCCTCCAATCAACAGAAGC+AGG	+	chr3.1:62269821-62269840	None:intergenic	45.0%
!	CCACGAGTCCTTTAAGTTTC+AGG	+	chr3.1:62270005-62270024	None:intergenic	45.0%
!	GGAGTATTTACTGGCGATTG+TGG	-	chr3.1:62270864-62270883	MS.gene052628:intron	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	+	chr3.1:62271063-62271082	None:intergenic	45.0%
!!	AAAAATATAATATATAACTT+AGG	+	chr3.1:62270155-62270174	None:intergenic	5.0%
	ATCAGATCCTCCGGCATCTA+TGG	+	chr3.1:62270668-62270687	None:intergenic	50.0%
	CTATGGCTACGGATACAGGT+TGG	+	chr3.1:62270651-62270670	None:intergenic	50.0%
	GCATCTATGGCTACGGATAC+AGG	+	chr3.1:62270655-62270674	None:intergenic	50.0%
	GTATCCGTAGCCATAGATGC+CGG	-	chr3.1:62270655-62270674	MS.gene052628:intron	50.0%
	GTCAAGGATCAAGGCCAATG+TGG	-	chr3.1:62269860-62269879	MS.gene052628:CDS	50.0%
	GTCACTGAGGTCAAGGATCA+AGG	-	chr3.1:62269851-62269870	MS.gene052628:CDS	50.0%
!	CTCCTGCTTCTGTTGATTGG+AGG	-	chr3.1:62269816-62269835	MS.gene052628:CDS	50.0%
!	CTGTTGACTGCGTGAATGCA+TGG	+	chr3.1:62269925-62269944	None:intergenic	50.0%
!!	AGGATGCAATGGTGGGCTAA+TGG	-	chr3.1:62270353-62270372	MS.gene052628:CDS	50.0%
!!	AGGTCCTGCTGGATTTTTGG+AGG	+	chr3.1:62271081-62271100	None:intergenic	50.0%
!!	GAAGAAAGGTGCTGTCACTG+AGG	-	chr3.1:62269838-62269857	MS.gene052628:CDS	50.0%
	AACTCATGGGGAGCGGAATG+GGG	-	chr3.1:62270963-62270982	MS.gene052628:CDS	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	-	chr3.1:62270962-62270981	MS.gene052628:CDS	55.0%
	TCCTCCGGCATCTATGGCTA+CGG	+	chr3.1:62270662-62270681	None:intergenic	55.0%
	TGTGAGGAACTCATGGGGAG+CGG	-	chr3.1:62270956-62270975	MS.gene052628:CDS	55.0%
!	AGGTGCTGTCACTGAGGTCA+AGG	-	chr3.1:62269844-62269863	MS.gene052628:CDS	55.0%
!!	GGCTACGGATACAGGTTGGT+TGG	+	chr3.1:62270647-62270666	None:intergenic	55.0%
	GCGTGCCTCGGAACATTCTG+TGG	+	chr3.1:62269754-62269773	None:intergenic	60.0%
	GGAACTCATGGGGAGCGGAA+TGG	-	chr3.1:62270961-62270980	MS.gene052628:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	-	chr3.1:62270972-62270991	MS.gene052628:CDS	60.0%
	TCCGTAGCCATAGATGCCGG+AGG	-	chr3.1:62270658-62270677	MS.gene052628:intron	60.0%
	ATGTTCCGAGGCACGCCACG+TGG	-	chr3.1:62269758-62269777	MS.gene052628:CDS	65.0%
	CACTTCCACGTGGCGTGCCT+CGG	+	chr3.1:62269766-62269785	None:intergenic	65.0%
	CGAGGCACGCCACGTGGAAG+TGG	-	chr3.1:62269764-62269783	MS.gene052628:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	62269659	62271127	62269659	ID=MS.gene052628
chr3.1	mRNA	62269659	62271127	62269659	ID=MS.gene052628.t1;Parent=MS.gene052628
chr3.1	exon	62270905	62271127	62270905	ID=MS.gene052628.t1.exon1;Parent=MS.gene052628.t1
chr3.1	CDS	62270905	62271127	62270905	ID=cds.MS.gene052628.t1;Parent=MS.gene052628.t1
chr3.1	exon	62270316	62270551	62270316	ID=MS.gene052628.t1.exon2;Parent=MS.gene052628.t1
chr3.1	CDS	62270316	62270551	62270316	ID=cds.MS.gene052628.t1;Parent=MS.gene052628.t1
chr3.1	exon	62270081	62270221	62270081	ID=MS.gene052628.t1.exon3;Parent=MS.gene052628.t1
chr3.1	CDS	62270081	62270221	62270081	ID=cds.MS.gene052628.t1;Parent=MS.gene052628.t1
chr3.1	exon	62269659	62269922	62269659	ID=MS.gene052628.t1.exon4;Parent=MS.gene052628.t1
chr3.1	CDS	62269659	62269922	62269659	ID=cds.MS.gene052628.t1;Parent=MS.gene052628.t1

Gene Sequence

>MS.gene052628

ATGGATAAGCCTTACAAGTTGAAACTTAACAAGTTTGCCGACATGACCAACCATGAATTTAGGACTACCTATGCTGGATCAAAGGTTAATCACCACAGAATGTTCCGAGGCACGCCACGTGGAAGTGGAACATTCATGTATGAGAATTTCACCAAAGCTCCTGCTTCTGTTGATTGGAGGAAGAAAGGTGCTGTCACTGAGGTCAAGGATCAAGGCCAATGTGGTAAGTTAAAAAAAGTTACTATTTAGATTCTTTAACATCACCATGCATTCACGCAGTCAACAGTTTGACACCATCTGATCAAAGATCGGATGACTCTTGTTACGAGCAATCTGTTGCAAACCTGAAACTTAAAGGACTCGTGGTGTAATTTAGCCTATTTATAAATTCAATTCACTTATTGACTACATAAATGCATAGGATTTTTGACTGAAGTCAACCTATTTTCAAATAGTTATACTAGTCATTAATAATCATATTCATAGAGCTTAACCTAAGTTATATATTATATTTTTCCACTATTATGCACTATAGCTTTTTATAACATTGTTGTTTGTGATTAATGTTTTGTACAGGTAGTTGCTGGGCTTTTTCAACTGTTGTAGCTGTTGAAGGCATAAACCAAATAAAGACAGATAGGTTAGTGCCTTTGTCTGAACAAGAGCTTATAGATTGTGACAATCAAGAAAATCAAGGATGCAATGGTGGGCTAATGGAATATGCTTTTGAGTACATTAAGCAAAAAGGTGGCTTAACAACAGAAAGCTATTACCCTTACACAGCAAATGATGGATCATGTGATGCAACAAAAGTGAGTAAAAGATGCTAAACTTCTATTGAAAATTTTTAATATAATTGACATGCAACTTTAGGAACCTAAGATTCAAAAATTGTTCATGTTGCAGGAAAATGTCCCAGCTGTATCAATTGATGGTCATGAGACTGTTCCGGCTAACGATGAAGATGCTTTGCTCAAAGCTGTTGCCAACCAACCTGTATCCGTAGCCATAGATGCCGGAGGATCTGATTTCCAGTTTTACTCGGAGGTAAAAATGAGAATGAATCCTCTCTATTGACACAAGAGAATAATGCAATCTAAACCTTCAAAATAAAAAAGTAAAAAAAGAGATGGAAGAAATATGCTATTATTAGCATTCAATTTTGAAACTAATGTGTATCTTAATTTTGTATACACCTATTACAGGGAGTATTTACTGGCGATTGTGGTAAAGAGCTAAATCATGGTGTAGCAATAGTAGGATATGGAACAACCGTTGATGGAACTAATTATTGGATTGTGAGGAACTCATGGGGAGCGGAATGGGGTGAACAAGGTTACATCAGAATGCAAAGGAATGTATCTAACAAGGAAGGACTTTGTGGCATAGCTATGGAAGCTTCCTACCCAGTTAAAAACTCCTCCAAAAATCCAGCAGGACCTTTATCATCTACCAAGGATGAGCTTTGA

Protein sequence

>MS.gene052628.t1

MDKPYKLKLNKFADMTNHEFRTTYAGSKVNHHRMFRGTPRGSGTFMYENFTKAPASVDWRKKGAVTEVKDQGQCGSCWAFSTVVAVEGINQIKTDRLVPLSEQELIDCDNQENQGCNGGLMEYAFEYIKQKGGLTTESYYPYTANDGSCDATKENVPAVSIDGHETVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCGKELNHGVAIVGYGTTVDGTNYWIVRNSWGAEWGEQGYIRMQRNVSNKEGLCGIAMEASYPVKNSSKNPAGPLSSTKDEL