Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63607.t1 | AES65228.2 | 91 | 100 | 9 | 0 | 3 | 102 | 128 | 227 | 7.70E-43 | 183.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63607.t1 | Q0JBP5 | 75.0 | 32 | 8 | 0 | 103 | 134 | 56 | 87 | 8.4e-06 | 51.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63607.t1 | A0A072V727 | 91.0 | 100 | 9 | 0 | 3 | 102 | 64 | 163 | 5.5e-43 | 183.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055091 | MS.gene63607 | 0.80338 | 3.58E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene63607 | 0.824223 | 9.07E-54 | -1.69E-46 |
| MS.gene059276 | MS.gene63607 | 0.803062 | 4.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63607.t1 | MTR_2g036890 | 90.099 | 101 | 10 | 0 | 2 | 102 | 63 | 163 | 2.97e-57 | 188 |
| MS.gene63607.t1 | MTR_2g036890 | 90.099 | 101 | 10 | 0 | 2 | 102 | 63 | 163 | 3.41e-57 | 189 |
| MS.gene63607.t1 | MTR_2g036890 | 90.099 | 101 | 10 | 0 | 2 | 102 | 127 | 227 | 5.55e-57 | 189 |
| MS.gene63607.t1 | MTR_2g012650 | 88.776 | 98 | 11 | 0 | 5 | 102 | 66 | 163 | 3.95e-55 | 182 |
| MS.gene63607.t1 | MTR_2g012650 | 88.776 | 98 | 11 | 0 | 5 | 102 | 66 | 163 | 4.36e-55 | 182 |
| MS.gene63607.t1 | MTR_2g012650 | 88.776 | 98 | 11 | 0 | 5 | 102 | 66 | 163 | 5.51e-55 | 182 |
| MS.gene63607.t1 | MTR_2g012650 | 88.776 | 98 | 11 | 0 | 5 | 102 | 155 | 252 | 1.07e-54 | 182 |
| MS.gene63607.t1 | MTR_2g012650 | 88.776 | 98 | 11 | 0 | 5 | 102 | 154 | 251 | 1.42e-54 | 182 |
| MS.gene63607.t1 | MTR_4g415340 | 70.408 | 98 | 12 | 1 | 5 | 102 | 66 | 146 | 1.14e-38 | 132 |
| MS.gene63607.t1 | MTR_1g015830 | 93.750 | 32 | 2 | 0 | 103 | 134 | 37 | 68 | 3.39e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGAATTTCAGGTTAAA+AGG | 0.298794 | 3.2:-24346808 | MS.gene63607:intron |
| ATTCAGATGAAGAGGATATT+AGG | 0.300475 | 3.2:-24347746 | MS.gene63607:CDS |
| AATTCTGATGACAATGGTTC+TGG | 0.331848 | 3.2:-24347813 | MS.gene63607:CDS |
| AGCTGAATTTCAAGTTCTCT+TGG | 0.357070 | 3.2:+24347259 | None:intergenic |
| TGGTGGAAGTGTTGGATTGA+TGG | 0.357231 | 3.2:-24346765 | MS.gene63607:CDS |
| ATATCTCAGAAAATGTATAA+TGG | 0.425060 | 3.2:-24346737 | MS.gene63607:CDS |
| AATTGCCAATGGACAGGAAC+TGG | 0.442067 | 3.2:-24347210 | MS.gene63607:CDS |
| AATGGTTCTGGACTAATAGA+AGG | 0.461206 | 3.2:-24347801 | MS.gene63607:CDS |
| GAAGAGGATATTAGGACAAA+TGG | 0.473711 | 3.2:-24347738 | MS.gene63607:CDS |
| GTGTATGGTGGTGGAAGTGT+TGG | 0.474413 | 3.2:-24346773 | MS.gene63607:CDS |
| AGCCACATCTGAGGATTTAG+TGG | 0.476918 | 3.2:-24347712 | MS.gene63607:CDS |
| CAATTTGAGACACCGACAAC+TGG | 0.481431 | 3.2:+24347228 | None:intergenic |
| AGCTGGGATGTACCAGTTGT+CGG | 0.485709 | 3.2:-24347240 | MS.gene63607:CDS |
| AAGAGAACTTGAAATTCAGC+TGG | 0.485994 | 3.2:-24347257 | MS.gene63607:CDS |
| TTTCGCCAGTTCCTGTCCAT+TGG | 0.496888 | 3.2:+24347205 | None:intergenic |
| GTCTCAAATTGCCAATGGAC+AGG | 0.501435 | 3.2:-24347216 | MS.gene63607:CDS |
| GGTTAAAAGGAACATATACT+TGG | 0.504303 | 3.2:-24346795 | MS.gene63607:CDS |
| AACTGGCGAAAATATATTCA+AGG | 0.505745 | 3.2:-24347193 | MS.gene63607:intron |
| CTCATTGTCCTCCTCAGTCT+CGG | 0.508233 | 3.2:+24347776 | None:intergenic |
| ATGGCTAGAATTAATGTTGT+GGG | 0.512597 | 3.2:-24347849 | MS.gene63607:CDS |
| AGGAACATATACTTGGTGTA+TGG | 0.516067 | 3.2:-24346788 | MS.gene63607:CDS |
| CTGAGGATTTAGTGGATCTG+AGG | 0.530617 | 3.2:-24347704 | MS.gene63607:intron |
| CAGCAAAATTCTGATGACAA+TGG | 0.555565 | 3.2:-24347819 | MS.gene63607:CDS |
| AATGGCTAGAATTAATGTTG+TGG | 0.564684 | 3.2:-24347850 | MS.gene63607:CDS |
| ATATACTTGGTGTATGGTGG+TGG | 0.570706 | 3.2:-24346782 | MS.gene63607:CDS |
| TCGGTGTCTCAAATTGCCAA+TGG | 0.599064 | 3.2:-24347221 | MS.gene63607:CDS |
| GAGGATATTAGGACAAATGG+TGG | 0.617892 | 3.2:-24347735 | MS.gene63607:CDS |
| AATAGAAGGTTCCGAGACTG+AGG | 0.628708 | 3.2:-24347787 | MS.gene63607:CDS |
| TCTCAGAAAATGTATAATGG+TGG | 0.630996 | 3.2:-24346734 | MS.gene63607:CDS |
| ATCCACTAAATCCTCAGATG+TGG | 0.632134 | 3.2:+24347710 | None:intergenic |
| AGAGAACTTGAAATTCAGCT+GGG | 0.638594 | 3.2:-24347256 | MS.gene63607:CDS |
| AAATGGTGGAGCCACATCTG+AGG | 0.640560 | 3.2:-24347721 | MS.gene63607:CDS |
| AGAAGGTTCCGAGACTGAGG+AGG | 0.656494 | 3.2:-24347784 | MS.gene63607:CDS |
| AACATATACTTGGTGTATGG+TGG | 0.677448 | 3.2:-24346785 | MS.gene63607:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATATATTTTCATATTAAGT+AGG | - | chr3.2:24347283-24347302 | MS.gene63607:CDS | 10.0% |
| !! | ATAAGTTATTCCAAAATTAT+TGG | - | chr3.2:24347808-24347827 | MS.gene63607:CDS | 15.0% |
| !!! | AATTATGAAAGTATTTAACA+TGG | + | chr3.2:24346836-24346855 | None:intergenic | 15.0% |
| !!! | TTGTATAAAAAAAGTATGAA+CGG | + | chr3.2:24346796-24346815 | None:intergenic | 15.0% |
| !! | ATATCAATTTGGTTATGTTT+GGG | - | chr3.2:24347701-24347720 | MS.gene63607:intron | 20.0% |
| !! | ATATCTCAGAAAATGTATAA+TGG | - | chr3.2:24347616-24347635 | MS.gene63607:intron | 20.0% |
| !! | TTTCTAATTGGAATAAAGAT+TGG | + | chr3.2:24346756-24346775 | None:intergenic | 20.0% |
| !!! | GTTATTGATTGATATCAATT+TGG | - | chr3.2:24347690-24347709 | MS.gene63607:intron | 20.0% |
| ! | AATGAATGAATACATCCTAT+TGG | + | chr3.2:24347310-24347329 | None:intergenic | 25.0% |
| ! | ATTCATTCATTTCCTTATAG+AGG | - | chr3.2:24347319-24347338 | MS.gene63607:intron | 25.0% |
| ! | GATATCAATTTGGTTATGTT+TGG | - | chr3.2:24347700-24347719 | MS.gene63607:intron | 25.0% |
| !! | AAAAAAAGTATGAACGGAAA+TGG | + | chr3.2:24346790-24346809 | None:intergenic | 25.0% |
| !! | AATCAAAACTTTTCTATCCA+AGG | + | chr3.2:24346706-24346725 | None:intergenic | 25.0% |
| !! | ATATATTTTCTTTGACACCT+TGG | - | chr3.2:24346686-24346705 | MS.gene63607:intron | 25.0% |
| !! | GAGAAACAAACCAATAATTT+TGG | + | chr3.2:24347821-24347840 | None:intergenic | 25.0% |
| !! | GCAAGATTTTCATTTCTAAT+TGG | + | chr3.2:24346768-24346787 | None:intergenic | 25.0% |
| !! | TTAATGTTGTTGTCTTTTTG+TGG | - | chr3.2:24347524-24347543 | MS.gene63607:intron | 25.0% |
| !!! | AGTTTTGAACTTACAAAATG+AGG | + | chr3.2:24347227-24347246 | None:intergenic | 25.0% |
| !!! | TATAGTGTTTGAGTTTGTTT+TGG | + | chr3.2:24347267-24347286 | None:intergenic | 25.0% |
| AACTGGCGAAAATATATTCA+AGG | - | chr3.2:24347160-24347179 | MS.gene63607:intron | 30.0% | |
| ATGGCTAGAATTAATGTTGT+GGG | - | chr3.2:24346504-24346523 | MS.gene63607:intron | 30.0% | |
| ATTCAGATGAAGAGGATATT+AGG | - | chr3.2:24346607-24346626 | MS.gene63607:intron | 30.0% | |
| GCTAGCTAGATAGTATTAAT+CGG | + | chr3.2:24346903-24346922 | None:intergenic | 30.0% | |
| GGTTAAAAGGAACATATACT+TGG | - | chr3.2:24347558-24347577 | MS.gene63607:intron | 30.0% | |
| TATGTAAGACTTCCTCTATA+AGG | + | chr3.2:24347334-24347353 | None:intergenic | 30.0% | |
| TCATATTAAGTAGGACCAAT+AGG | - | chr3.2:24347292-24347311 | MS.gene63607:CDS | 30.0% | |
| TCTCAGAAAATGTATAATGG+TGG | - | chr3.2:24347619-24347638 | MS.gene63607:intron | 30.0% | |
| TTCAAGGTATATAGTGCTAT+TGG | - | chr3.2:24347176-24347195 | MS.gene63607:intron | 30.0% | |
| ! | CTTTTTGTGGTTTGAATTTC+AGG | - | chr3.2:24347537-24347556 | MS.gene63607:intron | 30.0% |
| !! | GGTTTGAATTTCAGGTTAAA+AGG | - | chr3.2:24347545-24347564 | MS.gene63607:intron | 30.0% |
| AAGAGAACTTGAAATTCAGC+TGG | - | chr3.2:24347096-24347115 | MS.gene63607:intron | 35.0% | |
| AATTCTGATGACAATGGTTC+TGG | - | chr3.2:24346540-24346559 | MS.gene63607:intron | 35.0% | |
| AGAGAACTTGAAATTCAGCT+GGG | - | chr3.2:24347097-24347116 | MS.gene63607:intron | 35.0% | |
| CAGCAAAATTCTGATGACAA+TGG | - | chr3.2:24346534-24346553 | MS.gene63607:intron | 35.0% | |
| GAAGAGGATATTAGGACAAA+TGG | - | chr3.2:24346615-24346634 | MS.gene63607:intron | 35.0% | |
| GAATAAAGATTGGAGAGCTT+TGG | + | chr3.2:24346746-24346765 | None:intergenic | 35.0% | |
| GGATTCATTACAAATCATGC+AGG | + | chr3.2:24346882-24346901 | None:intergenic | 35.0% | |
| TATCCAAGATTTCTTGTCTC+TGG | - | chr3.2:24347201-24347220 | MS.gene63607:CDS | 35.0% | |
| ! | GTTTTCTCATTCAGATGAAG+AGG | - | chr3.2:24346599-24346618 | MS.gene63607:intron | 35.0% |
| !! | AACATATACTTGGTGTATGG+TGG | - | chr3.2:24347568-24347587 | MS.gene63607:intron | 35.0% |
| !! | AATGGTTCTGGACTAATAGA+AGG | - | chr3.2:24346552-24346571 | MS.gene63607:intron | 35.0% |
| !! | AGCTGAATTTCAAGTTCTCT+TGG | + | chr3.2:24347097-24347116 | None:intergenic | 35.0% |
| !! | AGGAACATATACTTGGTGTA+TGG | - | chr3.2:24347565-24347584 | MS.gene63607:intron | 35.0% |
| ATCCACTAAATCCTCAGATG+TGG | + | chr3.2:24346646-24346665 | None:intergenic | 40.0% | |
| GACGAACGAATTTGAGAAGA+GGG | + | chr3.2:24347413-24347432 | None:intergenic | 40.0% | |
| GAGGATATTAGGACAAATGG+TGG | - | chr3.2:24346618-24346637 | MS.gene63607:intron | 40.0% | |
| TGACGAACGAATTTGAGAAG+AGG | + | chr3.2:24347414-24347433 | None:intergenic | 40.0% | |
| !! | ATATACTTGGTGTATGGTGG+TGG | - | chr3.2:24347571-24347590 | MS.gene63607:intron | 40.0% |
| AATAGAAGGTTCCGAGACTG+AGG | - | chr3.2:24346566-24346585 | MS.gene63607:intron | 45.0% | |
| AATTGCCAATGGACAGGAAC+TGG | - | chr3.2:24347143-24347162 | MS.gene63607:intron | 45.0% | |
| AGCCACATCTGAGGATTTAG+TGG | - | chr3.2:24346641-24346660 | MS.gene63607:intron | 45.0% | |
| AGGCCAGAGACAAGAAATCT+TGG | + | chr3.2:24347207-24347226 | None:intergenic | 45.0% | |
| GTCTCAAATTGCCAATGGAC+AGG | - | chr3.2:24347137-24347156 | MS.gene63607:intron | 45.0% | |
| ! | CAATTTGAGACACCGACAAC+TGG | + | chr3.2:24347128-24347147 | None:intergenic | 45.0% |
| ! | CTGAGGATTTAGTGGATCTG+AGG | - | chr3.2:24346649-24346668 | MS.gene63607:intron | 45.0% |
| ! | TCGGTGTCTCAAATTGCCAA+TGG | - | chr3.2:24347132-24347151 | MS.gene63607:intron | 45.0% |
| !! | TGGTGGAAGTGTTGGATTGA+TGG | - | chr3.2:24347588-24347607 | MS.gene63607:intron | 45.0% |
| AGCTGGGATGTACCAGTTGT+CGG | - | chr3.2:24347113-24347132 | MS.gene63607:intron | 50.0% | |
| CTCATTGTCCTCCTCAGTCT+CGG | + | chr3.2:24346580-24346599 | None:intergenic | 50.0% | |
| TTTCGCCAGTTCCTGTCCAT+TGG | + | chr3.2:24347151-24347170 | None:intergenic | 50.0% | |
| ! | AAATGGTGGAGCCACATCTG+AGG | - | chr3.2:24346632-24346651 | MS.gene63607:intron | 50.0% |
| !! | GTGTATGGTGGTGGAAGTGT+TGG | - | chr3.2:24347580-24347599 | MS.gene63607:intron | 50.0% |
| AGAAGGTTCCGAGACTGAGG+AGG | - | chr3.2:24346569-24346588 | MS.gene63607:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 24346504 | 24347871 | 24346504 | ID=MS.gene63607 |
| chr3.2 | mRNA | 24346504 | 24347871 | 24346504 | ID=MS.gene63607.t1;Parent=MS.gene63607 |
| chr3.2 | exon | 24347705 | 24347871 | 24347705 | ID=MS.gene63607.t1.exon1;Parent=MS.gene63607.t1 |
| chr3.2 | CDS | 24347705 | 24347871 | 24347705 | ID=cds.MS.gene63607.t1;Parent=MS.gene63607.t1 |
| chr3.2 | exon | 24347194 | 24347332 | 24347194 | ID=MS.gene63607.t1.exon2;Parent=MS.gene63607.t1 |
| chr3.2 | CDS | 24347194 | 24347332 | 24347194 | ID=cds.MS.gene63607.t1;Parent=MS.gene63607.t1 |
| chr3.2 | exon | 24346700 | 24346816 | 24346700 | ID=MS.gene63607.t1.exon3;Parent=MS.gene63607.t1 |
| chr3.2 | CDS | 24346700 | 24346816 | 24346700 | ID=cds.MS.gene63607.t1;Parent=MS.gene63607.t1 |
| chr3.2 | exon | 24346504 | 24346509 | 24346504 | ID=MS.gene63607.t1.exon4;Parent=MS.gene63607.t1 |
| chr3.2 | CDS | 24346504 | 24346509 | 24346504 | ID=cds.MS.gene63607.t1;Parent=MS.gene63607.t1 |
| Gene Sequence |
| Protein sequence |
