Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055091.t1 | XP_027351310.1 | 60 | 90 | 35 | 1 | 56 | 144 | 144 | 233 | 3.50E-22 | 114.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055091.t1 | A0A445E7D2 | 57.8 | 90 | 37 | 1 | 56 | 144 | 139 | 228 | 9.0e-20 | 106.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051710 | MS.gene055091 | 0.802034 | 6.80E-49 | -1.69E-46 |
| MS.gene055091 | MS.gene058515 | 0.818182 | 2.24E-52 | -1.69E-46 |
| MS.gene055091 | MS.gene38429 | 0.817927 | 2.56E-52 | -1.69E-46 |
| MS.gene055091 | MS.gene46359 | 0.804754 | 1.86E-49 | -1.69E-46 |
| MS.gene055091 | MS.gene61742 | 0.853897 | 1.76E-61 | -1.69E-46 |
| MS.gene055091 | MS.gene63607 | 0.80338 | 3.58E-49 | -1.69E-46 |
| MS.gene055091 | MS.gene76987 | 0.808401 | 3.15E-50 | -1.69E-46 |
| MS.gene055091 | MS.gene78647 | 0.806813 | 6.86E-50 | -1.69E-46 |
| MS.gene055091 | MS.gene84612 | 0.806294 | 8.82E-50 | -1.69E-46 |
| MS.gene055091 | MS.gene84968 | 0.823057 | 1.70E-53 | -1.69E-46 |
| MS.gene055091 | MS.gene85436 | 0.803109 | 4.08E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCTTGCAGTTTGCATATT+GGG | 0.227948 | 7.4:-38393290 | MS.gene055091:CDS |
| ATTTGATTTCTTGTGGTTGC+AGG | 0.276775 | 7.4:+38394623 | None:intergenic |
| GTCCCTTGCAGTTTGCATAT+TGG | 0.312932 | 7.4:-38393291 | MS.gene055091:CDS |
| CAACCACAAGAAATCAAATA+TGG | 0.349528 | 7.4:-38394619 | MS.gene055091:intron |
| CAAGCACAGAGTAGTCTATA+AGG | 0.389984 | 7.4:+38393336 | None:intergenic |
| ACATTGGCTTTGCGTGTTCT+TGG | 0.422604 | 7.4:-38393236 | MS.gene055091:CDS |
| GGCTTTGCGTGTTCTTGGTA+AGG | 0.431277 | 7.4:-38393231 | MS.gene055091:CDS |
| TCAAAATATGCGTTGACAAA+CGG | 0.437688 | 7.4:+38394675 | None:intergenic |
| CATATTGGGTGAACGAGAGA+TGG | 0.458647 | 7.4:-38393276 | MS.gene055091:CDS |
| TTTGATTTCTTGTGGTTGCA+GGG | 0.485769 | 7.4:+38394624 | None:intergenic |
| GCTTCATTTGAGATTGGAAA+GGG | 0.489868 | 7.4:-38393116 | MS.gene055091:CDS |
| TCGTAGGCTTACTGTTGAGC+AGG | 0.504065 | 7.4:-38393165 | MS.gene055091:CDS |
| ACAGCTTCTGAGTACTTACA+AGG | 0.506273 | 7.4:+38393383 | None:intergenic |
| ATAATTACTAACCTATGTAC+TGG | 0.506330 | 7.4:-38394704 | None:intergenic |
| TCACCATATTTGATTTCTTG+TGG | 0.510515 | 7.4:+38394616 | None:intergenic |
| TTTCTCACCTTTCCCTTGGG+AGG | 0.518315 | 7.4:+38394410 | None:intergenic |
| TGCTTCATTTGAGATTGGAA+AGG | 0.521246 | 7.4:-38393117 | MS.gene055091:CDS |
| GAACGAGAGATGGGAAATGC+TGG | 0.522025 | 7.4:-38393266 | MS.gene055091:CDS |
| TTTCAAACTATATTGAACTT+CGG | 0.547944 | 7.4:-38393313 | MS.gene055091:CDS |
| AAATGCTGGACTTGAGACAT+TGG | 0.564065 | 7.4:-38393252 | MS.gene055091:CDS |
| CAACACTCCTCCCAAGGGAA+AGG | 0.566939 | 7.4:-38394417 | MS.gene055091:intron |
| ACTAACCTATGTACTGGTTG+AGG | 0.571028 | 7.4:-38394698 | None:intergenic |
| CACCCAATATGCAAACTGCA+AGG | 0.580961 | 7.4:+38393288 | None:intergenic |
| GGTGGATAGCTATGAGTAGG+TGG | 0.581024 | 7.4:-38393193 | MS.gene055091:CDS |
| CATATGCAACACTCCTCCCA+AGG | 0.586923 | 7.4:-38394423 | MS.gene055091:CDS |
| AAAGGGTTCTTTATTCTCGT+AGG | 0.591480 | 7.4:-38393099 | None:intergenic |
| GTGGATAGCTATGAGTAGGT+GGG | 0.595933 | 7.4:-38393192 | MS.gene055091:CDS |
| TGAGTAGGTGGGATAATCGT+AGG | 0.603697 | 7.4:-38393181 | MS.gene055091:CDS |
| CAAGGTGGATAGCTATGAGT+AGG | 0.604724 | 7.4:-38393196 | MS.gene055091:CDS |
| ATATTGGGTGAACGAGAGAT+GGG | 0.610011 | 7.4:-38393275 | MS.gene055091:CDS |
| ACCCAATATGCAAACTGCAA+GGG | 0.630150 | 7.4:+38393289 | None:intergenic |
| AACGGCCTCAACCAGTACAT+AGG | 0.634113 | 7.4:+38394693 | None:intergenic |
| GGAGTGTTGCATATGCTCGA+AGG | 0.640903 | 7.4:+38394431 | None:intergenic |
| CTACTCATAGCTATCCACCT+TGG | 0.643786 | 7.4:+38393197 | None:intergenic |
| ATATGCAACACTCCTCCCAA+GGG | 0.663884 | 7.4:-38394422 | MS.gene055091:CDS |
| GTAAGGATATCGATAAACCA+AGG | 0.685127 | 7.4:-38393214 | MS.gene055091:CDS |
| AGGATATCGATAAACCAAGG+TGG | 0.716367 | 7.4:-38393211 | MS.gene055091:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATAATAATAATAATAA+GGG | - | chr7.4:38393669-38393688 | MS.gene055091:intron | 0.0% |
| !! | TAATAATAATAATAATAATA+AGG | - | chr7.4:38393671-38393690 | MS.gene055091:intron | 0.0% |
| !! | AAAAAAAAAAAACAAAAATC+AGG | + | chr7.4:38393876-38393895 | None:intergenic | 10.0% |
| !!! | TATTTTTGAATTATGTAATT+CGG | - | chr7.4:38393908-38393927 | MS.gene055091:intron | 10.0% |
| !!! | AAAAAGTTGGTATATTTATA+GGG | + | chr7.4:38393736-38393755 | None:intergenic | 15.0% |
| !!! | AATTCCTTTTAATCTTATAT+AGG | + | chr7.4:38393828-38393847 | None:intergenic | 15.0% |
| !!! | ATTCCTTTTAATCTTATATA+GGG | + | chr7.4:38393827-38393846 | None:intergenic | 15.0% |
| !!! | TAGTGATTGAAAATTTTATA+TGG | + | chr7.4:38393796-38393815 | None:intergenic | 15.0% |
| !! | AAAATATCGATACTTCATTT+AGG | + | chr7.4:38394171-38394190 | None:intergenic | 20.0% |
| !! | AAATATCGATACTTCATTTA+GGG | + | chr7.4:38394170-38394189 | None:intergenic | 20.0% |
| !! | TTTCAAACTATATTGAACTT+CGG | - | chr7.4:38394477-38394496 | MS.gene055091:intron | 20.0% |
| !!! | AAATTTTCAATCACTACTTT+TGG | - | chr7.4:38393800-38393819 | MS.gene055091:intron | 20.0% |
| !!! | ATAAGGGTTAAATATGTTTT+TGG | - | chr7.4:38393709-38393728 | MS.gene055091:intron | 20.0% |
| !!! | ATATTTTATATGTATCCGAA+CGG | - | chr7.4:38394184-38394203 | MS.gene055091:intron | 20.0% |
| !!! | CAAAAAGTTGGTATATTTAT+AGG | + | chr7.4:38393737-38393756 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTCCTTTTTCTGA+CGG | - | chr7.4:38394332-38394351 | MS.gene055091:intron | 20.0% |
| ! | GTCAGACTTCATATTTAAAA+AGG | + | chr7.4:38393570-38393589 | None:intergenic | 25.0% |
| ! | TAATCTTCTCAAATTGTCTT+TGG | + | chr7.4:38393609-38393628 | None:intergenic | 25.0% |
| ! | TTAAATATGAAGTCTGACAT+AGG | - | chr7.4:38393572-38393591 | MS.gene055091:intron | 25.0% |
| ! | TTATTTATTCCGTCAGAAAA+AGG | + | chr7.4:38394344-38394363 | None:intergenic | 25.0% |
| !! | AAGTTGAAGGAAAATTTTAG+AGG | + | chr7.4:38393768-38393787 | None:intergenic | 25.0% |
| !! | AGTTGAAGGAAAATTTTAGA+GGG | + | chr7.4:38393767-38393786 | None:intergenic | 25.0% |
| !! | CTTAAATCTCTGATTTGAAT+AGG | + | chr7.4:38393636-38393655 | None:intergenic | 25.0% |
| !! | TTATATGGACTAAAAGTTGA+AGG | + | chr7.4:38393781-38393800 | None:intergenic | 25.0% |
| AAAGGAACAATGTGATGAAA+TGG | + | chr7.4:38393489-38393508 | None:intergenic | 30.0% | |
| CAACCACAAGAAATCAAATA+TGG | - | chr7.4:38393171-38393190 | MS.gene055091:CDS | 30.0% | |
| CATTGTATGATTAATTCCCA+AGG | + | chr7.4:38394299-38394318 | None:intergenic | 30.0% | |
| CTTTGTATGAATAATTCCCA+AGG | + | chr7.4:38394387-38394406 | None:intergenic | 30.0% | |
| GGTCCCTATATAAGATTAAA+AGG | - | chr7.4:38393821-38393840 | MS.gene055091:intron | 30.0% | |
| ! | TCAAAATATGCGTTGACAAA+CGG | + | chr7.4:38393118-38393137 | None:intergenic | 30.0% |
| ! | TCACCATATTTGATTTCTTG+TGG | + | chr7.4:38393177-38393196 | None:intergenic | 30.0% |
| ! | TTTAAAGTTCACGAGAAACT+CGG | + | chr7.4:38394037-38394056 | None:intergenic | 30.0% |
| !! | CTAAATTTTTGACTCGAGTT+CGG | + | chr7.4:38394064-38394083 | None:intergenic | 30.0% |
| !! | TTCTTTAACTTGCATTCTCA+AGG | + | chr7.4:38394262-38394281 | None:intergenic | 30.0% |
| AATACCATCAATGCAGCTTA+TGG | - | chr7.4:38393328-38393347 | MS.gene055091:CDS | 35.0% | |
| ACTTAATGATGTGGAGGAAA+TGG | + | chr7.4:38393282-38393301 | None:intergenic | 35.0% | |
| ATAGTAAACGTTGTAAGCCT+TGG | - | chr7.4:38394367-38394386 | MS.gene055091:intron | 35.0% | |
| ATTTCCTCCACATCATTAAG+TGG | - | chr7.4:38393281-38393300 | MS.gene055091:CDS | 35.0% | |
| GAAATACAAACGTATCCGTT+CGG | + | chr7.4:38394202-38394221 | None:intergenic | 35.0% | |
| GAGGAAATGGTCGTTAATAT+TGG | + | chr7.4:38393269-38393288 | None:intergenic | 35.0% | |
| GAGGGACTAAAACAAAAAGT+TGG | + | chr7.4:38393749-38393768 | None:intergenic | 35.0% | |
| GTAAGGATATCGATAAACCA+AGG | - | chr7.4:38394576-38394595 | MS.gene055091:intron | 35.0% | |
| TAGTAAACGTTGTAAGCCTT+GGG | - | chr7.4:38394368-38394387 | MS.gene055091:intron | 35.0% | |
| TTTCCTCCACATCATTAAGT+GGG | - | chr7.4:38393282-38393301 | MS.gene055091:CDS | 35.0% | |
| ! | ATTTGATTTCTTGTGGTTGC+AGG | + | chr7.4:38393170-38393189 | None:intergenic | 35.0% |
| ! | GATCTTGTTTATTGAAGCAG+AGG | + | chr7.4:38393432-38393451 | None:intergenic | 35.0% |
| ! | GCTTCATTTGAGATTGGAAA+GGG | - | chr7.4:38394674-38394693 | MS.gene055091:CDS | 35.0% |
| ! | GTTTGTCAACGCATATTTTG+AGG | - | chr7.4:38393117-38393136 | MS.gene055091:CDS | 35.0% |
| ! | TGCTTCATTTGAGATTGGAA+AGG | - | chr7.4:38394673-38394692 | MS.gene055091:CDS | 35.0% |
| ! | TTTGATTTCTTGTGGTTGCA+GGG | + | chr7.4:38393169-38393188 | None:intergenic | 35.0% |
| !! | GCTTTTGCTTCATTTGAGAT+TGG | - | chr7.4:38394668-38394687 | MS.gene055091:CDS | 35.0% |
| AAATGCTGGACTTGAGACAT+TGG | - | chr7.4:38394538-38394557 | MS.gene055091:intron | 40.0% | |
| ACAGCTTCTGAGTACTTACA+AGG | + | chr7.4:38394410-38394429 | None:intergenic | 40.0% | |
| ACCCAATATGCAAACTGCAA+GGG | + | chr7.4:38394504-38394523 | None:intergenic | 40.0% | |
| AGGATATCGATAAACCAAGG+TGG | - | chr7.4:38394579-38394598 | MS.gene055091:intron | 40.0% | |
| ATATTGGGTGAACGAGAGAT+GGG | - | chr7.4:38394515-38394534 | MS.gene055091:intron | 40.0% | |
| CAAGCACAGAGTAGTCTATA+AGG | + | chr7.4:38394457-38394476 | None:intergenic | 40.0% | |
| GAAAGTCCCACTTAATGATG+TGG | + | chr7.4:38393291-38393310 | None:intergenic | 40.0% | |
| TCCCTTGCAGTTTGCATATT+GGG | - | chr7.4:38394500-38394519 | MS.gene055091:intron | 40.0% | |
| TCTTCCATAAGCTGCATTGA+TGG | + | chr7.4:38393335-38393354 | None:intergenic | 40.0% | |
| TTTAGACCCACGAAACAGAT+CGG | + | chr7.4:38393531-38393550 | None:intergenic | 40.0% | |
| ! | AAATGAAGCGTCAAAGCCTT+GGG | - | chr7.4:38394280-38394299 | MS.gene055091:intron | 40.0% |
| ! | AGTTTTCTCACCTTTCCCTT+GGG | + | chr7.4:38393386-38393405 | None:intergenic | 40.0% |
| ! | CGACTCGATTCGATAAGAAT+TGG | + | chr7.4:38394109-38394128 | None:intergenic | 40.0% |
| ! | TAGTTTTCTCACCTTTCCCT+TGG | + | chr7.4:38393387-38393406 | None:intergenic | 40.0% |
| AGTCCCACTTAATGATGTGG+AGG | + | chr7.4:38393288-38393307 | None:intergenic | 45.0% | |
| AGTTCACGAGAAACTCGGTT+CGG | + | chr7.4:38394032-38394051 | None:intergenic | 45.0% | |
| ATATGCAACACTCCTCCCAA+GGG | - | chr7.4:38393368-38393387 | MS.gene055091:CDS | 45.0% | |
| ATTGGCCAGAAGGAGGTAAA+AGG | + | chr7.4:38393251-38393270 | None:intergenic | 45.0% | |
| CAAGGTGGATAGCTATGAGT+AGG | - | chr7.4:38394594-38394613 | MS.gene055091:intron | 45.0% | |
| CACCCAATATGCAAACTGCA+AGG | + | chr7.4:38394505-38394524 | None:intergenic | 45.0% | |
| CATATTGGGTGAACGAGAGA+TGG | - | chr7.4:38394514-38394533 | MS.gene055091:intron | 45.0% | |
| CGTTAATATTGGCCAGAAGG+AGG | + | chr7.4:38393258-38393277 | None:intergenic | 45.0% | |
| CTACTCATAGCTATCCACCT+TGG | + | chr7.4:38394596-38394615 | None:intergenic | 45.0% | |
| GGTCGTTAATATTGGCCAGA+AGG | + | chr7.4:38393261-38393280 | None:intergenic | 45.0% | |
| GTCCCTTGCAGTTTGCATAT+TGG | - | chr7.4:38394499-38394518 | MS.gene055091:intron | 45.0% | |
| GTGGATAGCTATGAGTAGGT+GGG | - | chr7.4:38394598-38394617 | MS.gene055091:intron | 45.0% | |
| TGAGTAGGTGGGATAATCGT+AGG | - | chr7.4:38394609-38394628 | MS.gene055091:intron | 45.0% | |
| TTGGCCAGAAGGAGGTAAAA+GGG | + | chr7.4:38393250-38393269 | None:intergenic | 45.0% | |
| ! | GAAATGAAGCGTCAAAGCCT+TGG | - | chr7.4:38394279-38394298 | MS.gene055091:intron | 45.0% |
| !! | ACATTGGCTTTGCGTGTTCT+TGG | - | chr7.4:38394554-38394573 | MS.gene055091:intron | 45.0% |
| CATATGCAACACTCCTCCCA+AGG | - | chr7.4:38393367-38393386 | MS.gene055091:CDS | 50.0% | |
| GAACGAGAGATGGGAAATGC+TGG | - | chr7.4:38394524-38394543 | MS.gene055091:intron | 50.0% | |
| GGCTTTGCGTGTTCTTGGTA+AGG | - | chr7.4:38394559-38394578 | MS.gene055091:intron | 50.0% | |
| GGTGGATAGCTATGAGTAGG+TGG | - | chr7.4:38394597-38394616 | MS.gene055091:intron | 50.0% | |
| TCGTAGGCTTACTGTTGAGC+AGG | - | chr7.4:38394625-38394644 | MS.gene055091:CDS | 50.0% | |
| TTTCTCACCTTTCCCTTGGG+AGG | + | chr7.4:38393383-38393402 | None:intergenic | 50.0% | |
| ! | CACTCCCTTTTACCTCCTTC+TGG | - | chr7.4:38393243-38393262 | MS.gene055091:CDS | 50.0% |
| !! | GGAGTGTTGCATATGCTCGA+AGG | + | chr7.4:38393362-38393381 | None:intergenic | 50.0% |
| AACAGATCGGAGGTCGGGAT+CGG | + | chr7.4:38393518-38393537 | None:intergenic | 55.0% | |
| AGACCCACGAAACAGATCGG+AGG | + | chr7.4:38393528-38393547 | None:intergenic | 55.0% | |
| CAACACTCCTCCCAAGGGAA+AGG | - | chr7.4:38393373-38393392 | MS.gene055091:CDS | 55.0% | |
| CACGAAACAGATCGGAGGTC+GGG | + | chr7.4:38393523-38393542 | None:intergenic | 55.0% | |
| CCACGAAACAGATCGGAGGT+CGG | + | chr7.4:38393524-38393543 | None:intergenic | 55.0% | |
| CGACCTCCGATCTGTTTCGT+GGG | - | chr7.4:38393522-38393541 | MS.gene055091:intron | 55.0% | |
| GGTCGGGATCGGTGAAGAAA+AGG | + | chr7.4:38393507-38393526 | None:intergenic | 55.0% | |
| !! | GAAACTCGGTTCGGCTCGTT+AGG | + | chr7.4:38394023-38394042 | None:intergenic | 55.0% |
| CCGACCTCCGATCTGTTTCG+TGG | - | chr7.4:38393521-38393540 | MS.gene055091:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 38393100 | 38394712 | 38393100 | ID=MS.gene055091 |
| chr7.4 | mRNA | 38393100 | 38394712 | 38393100 | ID=MS.gene055091.t1;Parent=MS.gene055091 |
| chr7.4 | exon | 38394620 | 38394712 | 38394620 | ID=MS.gene055091.t1.exon1;Parent=MS.gene055091.t1 |
| chr7.4 | CDS | 38394620 | 38394712 | 38394620 | ID=cds.MS.gene055091.t1;Parent=MS.gene055091.t1 |
| chr7.4 | exon | 38394418 | 38394468 | 38394418 | ID=MS.gene055091.t1.exon2;Parent=MS.gene055091.t1 |
| chr7.4 | CDS | 38394418 | 38394468 | 38394418 | ID=cds.MS.gene055091.t1;Parent=MS.gene055091.t1 |
| chr7.4 | exon | 38393100 | 38393405 | 38393100 | ID=MS.gene055091.t1.exon3;Parent=MS.gene055091.t1 |
| chr7.4 | CDS | 38393100 | 38393405 | 38393100 | ID=cds.MS.gene055091.t1;Parent=MS.gene055091.t1 |
| Gene Sequence |
| Protein sequence |
