AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060604

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060604.t1 MTR_2g076690 94.967 457 23 0 1 457 1 457 0.0 908
MS.gene060604.t1 MTR_2g076840 83.778 450 70 3 7 456 1 447 0.0 785
MS.gene060604.t1 MTR_2g076840 65.394 419 94 6 44 461 361 729 1.83e-172 502
MS.gene060604.t1 MTR_2g076920 79.375 480 73 4 1 461 1 473 0.0 778
MS.gene060604.t1 MTR_2g076710 84.152 448 62 3 3 443 1 446 0.0 773
MS.gene060604.t1 MTR_2g076970 81.153 451 84 1 7 457 1 450 0.0 769
MS.gene060604.t1 MTR_2g076900 80.043 461 84 3 7 461 1 459 0.0 765
MS.gene060604.t1 MTR_2g076990 82.785 395 66 1 65 457 1 395 0.0 669
MS.gene060604.t1 MTR_8g076920 45.969 459 232 5 10 455 19 474 6.74e-150 436
MS.gene060604.t1 MTR_5g020980 46.053 456 236 5 16 462 19 473 2.42e-146 427
MS.gene060604.t1 MTR_5g020990 41.778 450 236 7 21 447 36 482 8.31e-119 356
MS.gene060604.t1 MTR_4g107370 40.606 495 239 10 6 455 5 489 1.18e-114 347
MS.gene060604.t1 MTR_1g022250 42.827 467 230 10 6 445 2 458 5.16e-113 341
MS.gene060604.t1 MTR_2g030420 40.351 456 246 5 16 451 15 464 2.83e-112 339
MS.gene060604.t1 MTR_7g008040 40.741 459 225 9 16 435 10 460 7.30e-107 328
MS.gene060604.t1 MTR_6g009720 38.745 462 233 9 16 434 14 468 6.86e-105 322
MS.gene060604.t1 MTR_6g009520 40.260 462 220 11 16 435 14 461 3.56e-103 317
MS.gene060604.t1 MTR_6g009770 40.460 435 204 9 46 435 23 447 4.46e-103 317
MS.gene060604.t1 MTR_6g009553 39.606 457 213 11 30 435 4 448 2.28e-102 314
MS.gene060604.t1 MTR_4g128260 36.798 481 254 8 16 460 10 476 1.50e-101 313
MS.gene060604.t1 MTR_6g042290 39.207 454 239 11 5 435 12 451 3.29e-101 312
MS.gene060604.t1 MTR_6g009540 40.351 456 228 11 16 435 12 459 6.13e-101 311
MS.gene060604.t1 MTR_1g022245 37.660 470 242 9 7 436 1 459 7.83e-99 306
MS.gene060604.t1 MTR_2g061820 36.100 482 252 10 14 457 3 466 1.73e-98 305
MS.gene060604.t1 MTR_3g103150 50.179 279 131 4 162 435 1 276 3.32e-91 279
MS.gene060604.t1 MTR_6g009510 37.020 443 219 9 5 435 12 406 2.11e-87 275
MS.gene060604.t1 MTR_6g009660 38.107 412 213 12 43 435 23 411 1.33e-77 250
MS.gene060604.t1 MTR_3g092060 33.684 380 213 10 68 437 66 416 3.58e-68 226
MS.gene060604.t1 MTR_5g021020 34.491 432 232 10 10 435 19 405 4.43e-67 221
MS.gene060604.t1 MTR_7g117950 32.773 357 201 8 65 415 61 384 3.54e-64 214
MS.gene060604.t1 MTR_8g027200 29.771 393 213 12 54 435 48 388 1.07e-46 168
MS.gene060604.t1 MTR_2g076820 92.424 66 5 0 366 431 1 66 1.50e-36 129
MS.gene060604.t1 MTR_5g021000 61.905 105 38 1 333 435 1 105 3.96e-36 131
MS.gene060604.t1 MTR_5g020720 39.891 183 77 4 264 435 25 185 1.78e-31 120
MS.gene060604.t1 MTR_1g011680 43.902 123 69 0 318 440 14 136 8.36e-29 112
MS.gene060604.t1 MTR_2g076910 66.667 75 24 1 7 81 1 74 1.86e-27 105
MS.gene060604.t1 MTR_5g021010 49.524 105 45 3 339 435 17 121 4.13e-21 89.0
MS.gene060604.t1 MTR_2g076830 78.723 47 10 0 404 450 9 55 4.38e-17 75.9
MS.gene060604.t1 MTR_3g117230 30.047 213 131 7 190 393 129 332 7.90e-15 76.6
MS.gene060604.t1 MTR_3g112260 26.855 283 185 9 190 459 126 399 1.36e-14 75.9
MS.gene060604.t1 MTR_3g062510 26.804 194 127 6 216 401 171 357 1.71e-14 75.5
MS.gene060604.t1 MTR_3g062510 28.313 166 106 4 216 373 171 331 2.85e-14 74.7
MS.gene060604.t1 MTR_7g099640 30.270 185 109 8 210 383 194 369 2.18e-13 72.4
MS.gene060604.t1 MTR_7g099640 30.270 185 109 8 210 383 194 369 2.24e-13 72.0
MS.gene060604.t1 MTR_1g083330 30.488 164 99 5 230 384 193 350 5.74e-13 70.9
MS.gene060604.t1 MTR_4g033235 30.488 164 99 5 230 384 194 351 6.36e-13 70.9
MS.gene060604.t1 MTR_7g089040 32.026 153 88 6 228 372 408 552 8.48e-13 71.2
MS.gene060604.t1 MTR_4g094270 29.609 179 105 7 210 376 163 332 1.93e-12 69.3
MS.gene060604.t1 MTR_7g096060 29.319 191 122 5 206 388 242 427 4.12e-12 68.9
MS.gene060604.t1 MTR_8g099605 31.395 172 97 6 221 376 179 345 8.29e-12 67.8
MS.gene060604.t1 MTR_5g069780 31.214 173 106 5 204 368 313 480 8.70e-12 67.8
MS.gene060604.t1 MTR_8g099605 31.579 171 96 6 222 376 421 586 1.17e-11 67.8
MS.gene060604.t1 MTR_7g010800 28.495 186 117 6 201 377 203 381 2.02e-11 66.6
MS.gene060604.t1 MTR_3g462930 30.857 175 108 5 204 370 304 473 2.42e-11 66.6
MS.gene060604.t1 MTR_7g088850 29.762 168 101 6 217 372 291 453 3.21e-11 65.9
MS.gene060604.t1 MTR_3g023890 26.267 217 138 6 211 417 105 309 5.40e-11 65.5
MS.gene060604.t1 MTR_1g113750 41.053 95 50 3 366 454 16 110 5.43e-11 60.5
MS.gene060604.t1 MTR_1g063380 28.571 168 107 5 213 372 487 649 6.49e-11 65.1
MS.gene060604.t1 MTR_7g101870 24.096 249 144 9 161 373 136 375 7.14e-11 65.1
MS.gene060604.t1 MTR_7g101870 27.684 177 115 5 205 373 480 651 9.04e-11 64.7
MS.gene060604.t1 MTR_2g095210 26.378 254 154 11 213 451 415 650 8.88e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060604.t1 AT3G50940 53.237 417 194 1 25 440 30 446 4.10e-159 458
MS.gene060604.t1 AT3G50930 48.206 446 212 5 23 452 74 516 5.38e-148 434
MS.gene060604.t1 AT2G18193 43.722 446 223 5 16 447 20 451 3.93e-127 378
MS.gene060604.t1 AT5G17740 41.295 448 249 4 16 451 19 464 6.38e-122 366
MS.gene060604.t1 AT2G18190 42.151 465 240 6 4 450 5 458 5.41e-121 362
MS.gene060604.t1 AT5G17760 41.286 482 259 7 4 463 3 482 7.59e-121 362
MS.gene060604.t1 AT5G17730 41.256 446 239 10 10 439 9 447 1.66e-118 355
MS.gene060604.t1 AT3G28600 41.342 462 217 9 16 441 9 452 4.53e-114 344
MS.gene060604.t1 AT3G28510 40.263 457 231 8 16 438 13 461 7.88e-111 337
MS.gene060604.t1 AT3G28540 39.956 458 218 8 16 432 13 454 2.25e-109 333
MS.gene060604.t1 AT3G28540 39.956 458 218 8 16 432 13 454 3.27e-109 333
MS.gene060604.t1 AT3G28610 43.478 414 193 9 51 441 57 452 4.05e-108 329
MS.gene060604.t1 AT3G28580 39.355 465 238 10 1 431 1 455 4.72e-108 329
MS.gene060604.t1 AT4G05380 40.803 473 238 11 3 447 1 459 5.29e-108 328
MS.gene060604.t1 AT4G25835 39.492 433 228 6 16 420 10 436 6.67e-106 324
MS.gene060604.t1 AT5G40010 40.088 454 228 9 16 431 13 460 4.43e-104 320
MS.gene060604.t1 AT5G57480 35.870 460 251 6 16 437 10 463 1.63e-101 313
MS.gene060604.t1 AT5G40000 39.167 480 239 11 7 451 4 465 4.88e-101 310
MS.gene060604.t1 AT5G17750 39.759 415 210 9 3 403 2 390 1.78e-100 306
MS.gene060604.t1 AT3G28570 38.578 464 235 11 16 456 11 447 6.81e-100 307
MS.gene060604.t1 AT3G28570 37.996 479 246 12 2 456 3 454 7.45e-100 307
MS.gene060604.t1 AT3G28600 39.341 455 197 8 16 441 9 413 9.74e-99 303
MS.gene060604.t1 AT1G43910 38.065 465 242 7 10 443 6 455 1.17e-98 305
MS.gene060604.t1 AT3G28520 39.278 471 244 8 18 455 1 462 4.25e-97 300
MS.gene060604.t1 AT3G28580 38.789 446 245 10 1 431 1 433 2.70e-96 298
MS.gene060604.t1 AT4G30250 36.170 470 254 9 6 437 2 463 1.06e-95 298
MS.gene060604.t1 AT4G30250 36.034 469 254 9 7 437 1 461 5.45e-95 296
MS.gene060604.t1 AT2G46620 33.099 426 231 10 54 455 49 444 7.38e-74 241
MS.gene060604.t1 AT5G17760 39.506 324 179 4 4 312 3 324 1.20e-71 230
MS.gene060604.t1 AT3G29800 31.448 442 232 13 30 449 11 403 7.47e-64 213
MS.gene060604.t1 AT4G05340 58.824 68 28 0 323 390 26 93 6.32e-20 84.7
MS.gene060604.t1 AT3G28560 29.534 193 106 5 35 214 36 211 7.17e-18 83.6
MS.gene060604.t1 AT1G45000 28.521 284 179 11 190 459 126 399 4.73e-15 77.4
MS.gene060604.t1 AT5G58290 26.214 206 137 6 204 401 152 350 5.64e-15 77.0
MS.gene060604.t1 AT5G43010 30.256 195 119 6 190 376 126 311 7.31e-15 76.6
MS.gene060604.t1 AT3G05530 31.098 164 98 5 230 384 194 351 2.63e-13 72.0
MS.gene060604.t1 AT4G29040 30.337 178 105 7 217 383 198 367 4.36e-13 71.2
MS.gene060604.t1 AT2G20140 29.714 175 110 5 217 383 198 367 8.21e-13 70.5
MS.gene060604.t1 AT5G19990 29.050 179 106 7 210 376 107 276 1.31e-12 69.3
MS.gene060604.t1 AT5G19990 29.609 179 105 7 210 376 165 334 2.33e-12 68.9
MS.gene060604.t1 AT5G19990 29.609 179 105 7 210 376 165 334 2.33e-12 68.9
MS.gene060604.t1 AT5G20000 29.050 179 106 7 210 376 165 334 2.39e-12 68.9
MS.gene060604.t1 AT5G58870 30.857 175 108 5 204 370 326 495 9.10e-12 67.8
MS.gene060604.t1 AT4G23940 30.601 183 104 7 210 376 434 609 1.33e-11 67.4
MS.gene060604.t1 AT2G03670 31.013 158 85 6 234 376 49 197 1.44e-11 67.0
MS.gene060604.t1 AT2G03670 31.013 158 85 6 234 376 49 197 1.68e-11 66.2
MS.gene060604.t1 AT3G47060 30.508 177 110 5 204 372 322 493 4.34e-11 65.9

Find 71 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGATTGAAGTTCTTCT+TGG 0.223470 2.2:+17336114 MS.gene060604:CDS
CTTCTCTCTTAGTTTCTTCC+TGG 0.230380 2.2:-17336245 None:intergenic
TTTCAACAGATGACACTTTC+GGG 0.253746 2.2:+17335886 MS.gene060604:intron
ATTGGATGGTTGAACTTAAT+AGG 0.269533 2.2:-17335078 None:intergenic
TTGAAATTCTTCAATGACTA+TGG 0.286598 2.2:-17334656 None:intergenic
AAGGGAAATTAAGAATGATT+TGG 0.292518 2.2:+17335137 MS.gene060604:CDS
TTGTATGGTCCTCCTGGTAC+TGG 0.303664 2.2:+17335225 MS.gene060604:CDS
GATGAAGAAGTTGTTCATAA+TGG 0.310288 2.2:+17335426 MS.gene060604:CDS
CAACTTCTTCATCTTCTTCT+CGG 0.328790 2.2:-17335416 None:intergenic
CAAGAATTCGACATTATGAT+AGG 0.351643 2.2:+17334881 MS.gene060604:CDS
TGAATGCAGTAGATGGTCTT+TGG 0.365588 2.2:+17335914 MS.gene060604:CDS
TGTTCATAATGGGCACAATA+AGG 0.386190 2.2:+17335437 MS.gene060604:CDS
AGTTAAAGCAACCAAATCTA+AGG 0.392234 2.2:+17334753 MS.gene060604:CDS
ATGAAGAAGTTGTTCATAAT+GGG 0.392993 2.2:+17335427 MS.gene060604:CDS
ACCGCGTTTCCAAGCTTTCC+CGG 0.395418 2.2:-17335196 None:intergenic
AGTTGCTTTAGTTCCTAGAT+AGG 0.400041 2.2:-17334716 None:intergenic
GGAATTTGATAAGGTCTTGC+AGG 0.402644 2.2:-17336203 None:intergenic
GTCCCGATTGAACAATGTTT+AGG 0.406316 2.2:-17334597 None:intergenic
ACTTGGATCTCACTAATGTT+GGG 0.408726 2.2:+17335301 MS.gene060604:CDS
AACTCTTCTGCAATTTCAGC+TGG 0.411104 2.2:-17336153 None:intergenic
GAGGCAGCAGAAGCCTATCT+AGG 0.421960 2.2:+17334703 MS.gene060604:CDS
TGGCTTGGAGGAATTTGATA+AGG 0.429033 2.2:-17336212 None:intergenic
GAGGCCACTGCAGATAAAAC+TGG 0.436495 2.2:-17334526 None:intergenic
AGTCCTAAACATTGTTCAAT+CGG 0.445916 2.2:+17334594 MS.gene060604:CDS
CCGTTCTATACTTGTGATAG+AGG 0.447088 2.2:+17335365 MS.gene060604:CDS
GACTTGGATCTCACTAATGT+TGG 0.453484 2.2:+17335300 MS.gene060604:CDS
TTGAAGGTGTTAGTGTAAAG+TGG 0.455356 2.2:+17334830 MS.gene060604:CDS
TGGTGAATTGCGCGTTGAAT+TGG 0.456678 2.2:-17334636 None:intergenic
TACTTGTTGTATGGTCCTCC+TGG 0.457478 2.2:+17335219 MS.gene060604:CDS
GCAAGAGTGTTGAAACTCAT+TGG 0.462379 2.2:-17335096 None:intergenic
TTAGCTCTCTCCATCACATA+AGG 0.464927 2.2:-17334988 None:intergenic
TCACAACAAATCACAGAGAC+AGG 0.469535 2.2:+17335968 MS.gene060604:CDS
AAACGCGGTTACTTGTTGTA+TGG 0.474020 2.2:+17335210 MS.gene060604:CDS
ATAGAAGAACCGGGAAAGCT+TGG 0.478091 2.2:+17335187 MS.gene060604:CDS
AGCAAAGAAGAACAAGATCT+AGG 0.484846 2.2:+17336273 MS.gene060604:CDS
CAATTGATGAAGAGCTTCAA+AGG 0.486235 2.2:+17335118 MS.gene060604:CDS
CCAAGCCAAGAAAATGATCC+AGG 0.489155 2.2:+17336227 MS.gene060604:CDS
ATAGTCATTGAAGAATTTCA+AGG 0.496242 2.2:+17334658 MS.gene060604:CDS
GTCTAGCTTGATTGCAGCCA+TGG 0.496694 2.2:+17335251 MS.gene060604:CDS
AAGCTAGACTTCCCAGTACC+AGG 0.497146 2.2:-17335237 None:intergenic
GGACTATTGAATGCAGTAGA+TGG 0.497808 2.2:+17335907 MS.gene060604:CDS
GTCCTAAACATTGTTCAATC+GGG 0.500773 2.2:+17334595 MS.gene060604:CDS
GATCTAGGCAGAGAAAATAT+AGG 0.509105 2.2:+17336288 MS.gene060604:CDS
CAACTATGATATCTATGACT+TGG 0.513130 2.2:+17335284 MS.gene060604:CDS
ATTTGCTTGTCCATTCTACC+AGG 0.513687 2.2:-17336012 None:intergenic
AGTTCGCATTAGCGCAATTG+AGG 0.515687 2.2:-17334545 None:intergenic
TTCATATTTGCCTTATGTGA+TGG 0.523892 2.2:+17334978 MS.gene060604:CDS
TGTATGGTCCTCCTGGTACT+GGG 0.528478 2.2:+17335226 MS.gene060604:CDS
AATTGATGAAGAGCTTCAAA+GGG 0.533769 2.2:+17335119 MS.gene060604:CDS
TCTGTTAAGACCTGGTAGAA+TGG 0.545715 2.2:+17336002 MS.gene060604:CDS
CTACCAGGTCTTAACAGAGC+AGG 0.546075 2.2:-17335997 None:intergenic
TATCACAAGTATAGAACGGT+TGG 0.555140 2.2:-17335361 None:intergenic
ACAAATTAAACAAGGAATTG+TGG 0.559399 2.2:+17335011 MS.gene060604:CDS
AAGAATTCGACATTATGATA+GGG 0.568700 2.2:+17334882 MS.gene060604:CDS
ACTCAAATGAATATAATCGT+TGG 0.570247 2.2:+17335046 MS.gene060604:CDS
ACATTATGATAGGGACTCCT+CGG 0.578511 2.2:+17334891 MS.gene060604:CDS
CTTGGATCTCACTAATGTTG+GGG 0.595649 2.2:+17335302 MS.gene060604:CDS
GATCCTGCTCTGTTAAGACC+TGG 0.601081 2.2:+17335994 MS.gene060604:CDS
GTTCATGTTCAGTAATGCAA+AGG 0.603050 2.2:-17336086 None:intergenic
TAGTCATTGAAGAATTTCAA+GGG 0.606257 2.2:+17334659 MS.gene060604:CDS
GAGTGTTGAAACTCATTGGA+TGG 0.615289 2.2:-17335092 None:intergenic
ACCGGGAAAGCTTGGAAACG+CGG 0.621197 2.2:+17335195 MS.gene060604:CDS
TCTTATTTCTGACACAGCCG+AGG 0.630279 2.2:-17334908 None:intergenic
CCTCTATCACAAGTATAGAA+CGG 0.641804 2.2:-17335365 None:intergenic
ATAGTTGAGATAGTTAGCCA+TGG 0.661661 2.2:-17335268 None:intergenic
GATGGTCTTTGGTCATGCTG+TGG 0.668698 2.2:+17335925 MS.gene060604:CDS
ACAATGTTTAGGACTTCACG+AGG 0.686927 2.2:-17334586 None:intergenic
AGAGCTAAACAAATTAAACA+AGG 0.691321 2.2:+17335003 MS.gene060604:CDS
AATTGCAGAAGAGTTAACAA+AGG 0.702150 2.2:+17336161 MS.gene060604:CDS
TCTTTGGTCATGCTGTGGAG+AGG 0.703389 2.2:+17335930 MS.gene060604:CDS
CTAGACTTCCCAGTACCAGG+AGG 0.750100 2.2:-17335234 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTAGCTGAAAAAATT+TGG + chr2.2:17335639-17335658 MS.gene060604:intron 15.0%
!! TCTTTCAAATAATTACAGTT+TGG - chr2.2:17335511-17335530 None:intergenic 20.0%
!!! AAAGAATTTTATAGAAGAAC+CGG + chr2.2:17335177-17335196 MS.gene060604:CDS 20.0%
!!! ATTAGTTTAAAATAGTTGAC+AGG - chr2.2:17335852-17335871 None:intergenic 20.0%
! AAGAATTCGACATTATGATA+GGG + chr2.2:17334882-17334901 MS.gene060604:CDS 25.0%
! AAGGGAAATTAAGAATGATT+TGG + chr2.2:17335137-17335156 MS.gene060604:CDS 25.0%
! ACAAATTAAACAAGGAATTG+TGG + chr2.2:17335011-17335030 MS.gene060604:CDS 25.0%
! ACTCAAATGAATATAATCGT+TGG + chr2.2:17335046-17335065 MS.gene060604:CDS 25.0%
! AGAGCTAAACAAATTAAACA+AGG + chr2.2:17335003-17335022 MS.gene060604:CDS 25.0%
! ATAGTCATTGAAGAATTTCA+AGG + chr2.2:17334658-17334677 MS.gene060604:CDS 25.0%
! ATGAAGAAGTTGTTCATAAT+GGG + chr2.2:17335427-17335446 MS.gene060604:CDS 25.0%
! TAGTCATTGAAGAATTTCAA+GGG + chr2.2:17334659-17334678 MS.gene060604:CDS 25.0%
! TTGAAATTCTTCAATGACTA+TGG - chr2.2:17334659-17334678 None:intergenic 25.0%
!! AAGAATTTTATAGAAGAACC+GGG + chr2.2:17335178-17335197 MS.gene060604:CDS 25.0%
!! CTTTTTATGTTCCTTAGATT+TGG - chr2.2:17334767-17334786 None:intergenic 25.0%
!!! CTTTTCTGCTTTTAAGAAAT+TGG + chr2.2:17336053-17336072 MS.gene060604:CDS 25.0%
AATTGATGAAGAGCTTCAAA+GGG + chr2.2:17335119-17335138 MS.gene060604:CDS 30.0%
AATTGCAGAAGAGTTAACAA+AGG + chr2.2:17336161-17336180 MS.gene060604:CDS 30.0%
AATTTGACGTACATGAAATC+TGG + chr2.2:17335530-17335549 MS.gene060604:intron 30.0%
ACAGGAACAAAAGAATATGT+TGG - chr2.2:17335834-17335853 None:intergenic 30.0%
AGTCCTAAACATTGTTCAAT+CGG + chr2.2:17334594-17334613 MS.gene060604:CDS 30.0%
AGTTAAAGCAACCAAATCTA+AGG + chr2.2:17334753-17334772 MS.gene060604:CDS 30.0%
ATGATGTCTGTTCATATCAT+GGG + chr2.2:17335790-17335809 MS.gene060604:intron 30.0%
ATTGGATGGTTGAACTTAAT+AGG - chr2.2:17335081-17335100 None:intergenic 30.0%
CAACTATGATATCTATGACT+TGG + chr2.2:17335284-17335303 MS.gene060604:CDS 30.0%
CAAGAATTCGACATTATGAT+AGG + chr2.2:17334881-17334900 MS.gene060604:CDS 30.0%
GAAAAGATTGAAGTTCTTCT+TGG + chr2.2:17336114-17336133 MS.gene060604:CDS 30.0%
GAAGTCATGTTGTTAACAAT+AGG - chr2.2:17335603-17335622 None:intergenic 30.0%
GATGAAGAAGTTGTTCATAA+TGG + chr2.2:17335426-17335445 MS.gene060604:CDS 30.0%
TATGATGTCTGTTCATATCA+TGG + chr2.2:17335789-17335808 MS.gene060604:intron 30.0%
TTCATATTTGCCTTATGTGA+TGG + chr2.2:17334978-17334997 MS.gene060604:CDS 30.0%
! AAGATTTTGTCGACAATTGT+TGG + chr2.2:17335672-17335691 MS.gene060604:intron 30.0%
! TTCTCGGTTTTGCAATTTTA+CGG - chr2.2:17335403-17335422 None:intergenic 30.0%
! TTTTCAACAGATGACACTTT+CGG + chr2.2:17335885-17335904 MS.gene060604:intron 30.0%
!! CTGAAAAAATTTGGTCTTGT+TGG + chr2.2:17335648-17335667 MS.gene060604:intron 30.0%
AATCATCTGCATGATTTAGC+CGG + chr2.2:17335566-17335585 MS.gene060604:intron 35.0%
ACAATAGGACCAAAATTCAC+CGG - chr2.2:17335588-17335607 None:intergenic 35.0%
ACTTGGATCTCACTAATGTT+GGG + chr2.2:17335301-17335320 MS.gene060604:CDS 35.0%
AGCAAAGAAGAACAAGATCT+AGG + chr2.2:17336273-17336292 MS.gene060604:CDS 35.0%
ATAGTTGAGATAGTTAGCCA+TGG - chr2.2:17335271-17335290 None:intergenic 35.0%
CAACTTCTTCATCTTCTTCT+CGG - chr2.2:17335419-17335438 None:intergenic 35.0%
CAATTGATGAAGAGCTTCAA+AGG + chr2.2:17335118-17335137 MS.gene060604:CDS 35.0%
CCTCTATCACAAGTATAGAA+CGG - chr2.2:17335368-17335387 None:intergenic 35.0%
CGTTGAATTGGCAAAAAAGA+TGG - chr2.2:17334627-17334646 None:intergenic 35.0%
CTAAATATTATGTCCTCGTG+CGG - chr2.2:17335628-17335647 None:intergenic 35.0%
GATCTAGGCAGAGAAAATAT+AGG + chr2.2:17336288-17336307 MS.gene060604:CDS 35.0%
GTCCTAAACATTGTTCAATC+GGG + chr2.2:17334595-17334614 MS.gene060604:CDS 35.0%
GTTCATGTTCAGTAATGCAA+AGG - chr2.2:17336089-17336108 None:intergenic 35.0%
TATCACAAGTATAGAACGGT+TGG - chr2.2:17335364-17335383 None:intergenic 35.0%
TGATGTCTGTTCATATCATG+GGG + chr2.2:17335791-17335810 MS.gene060604:intron 35.0%
TTTCAACAGATGACACTTTC+GGG + chr2.2:17335886-17335905 MS.gene060604:intron 35.0%
! AGTTGCTTTAGTTCCTAGAT+AGG - chr2.2:17334719-17334738 None:intergenic 35.0%
! ATGATTTAGCCGGTGAATTT+TGG + chr2.2:17335576-17335595 MS.gene060604:intron 35.0%
! GACAAGAAACCAAGTTTTTG+AGG + chr2.2:17334684-17334703 MS.gene060604:CDS 35.0%
! TTCTTCCTGGATCATTTTCT+TGG - chr2.2:17336235-17336254 None:intergenic 35.0%
!! GAAGTGAGTGATGTTTTTGA+AGG + chr2.2:17334814-17334833 MS.gene060604:CDS 35.0%
!! TGTTCATAATGGGCACAATA+AGG + chr2.2:17335437-17335456 MS.gene060604:CDS 35.0%
!! TTGAAGGTGTTAGTGTAAAG+TGG + chr2.2:17334830-17334849 MS.gene060604:CDS 35.0%
AACTCTTCTGCAATTTCAGC+TGG - chr2.2:17336156-17336175 None:intergenic 40.0%
ACATTATGATAGGGACTCCT+CGG + chr2.2:17334891-17334910 MS.gene060604:CDS 40.0%
ATTTGCTTGTCCATTCTACC+AGG - chr2.2:17336015-17336034 None:intergenic 40.0%
CAAACTATGTACATACGACG+AGG + chr2.2:17335724-17335743 MS.gene060604:intron 40.0%
CCGTTCTATACTTGTGATAG+AGG + chr2.2:17335365-17335384 MS.gene060604:CDS 40.0%
CTTCTCTCTTAGTTTCTTCC+TGG - chr2.2:17336248-17336267 None:intergenic 40.0%
CTTGGATCTCACTAATGTTG+GGG + chr2.2:17335302-17335321 MS.gene060604:CDS 40.0%
GACTTGGATCTCACTAATGT+TGG + chr2.2:17335300-17335319 MS.gene060604:CDS 40.0%
GGAATTTGATAAGGTCTTGC+AGG - chr2.2:17336206-17336225 None:intergenic 40.0%
GGACTATTGAATGCAGTAGA+TGG + chr2.2:17335907-17335926 MS.gene060604:CDS 40.0%
GTCCCGATTGAACAATGTTT+AGG - chr2.2:17334600-17334619 None:intergenic 40.0%
TCACAACAAATCACAGAGAC+AGG + chr2.2:17335968-17335987 MS.gene060604:CDS 40.0%
TCTGTTAAGACCTGGTAGAA+TGG + chr2.2:17336002-17336021 MS.gene060604:CDS 40.0%
TGAATGCAGTAGATGGTCTT+TGG + chr2.2:17335914-17335933 MS.gene060604:CDS 40.0%
! ACAATGTTTAGGACTTCACG+AGG - chr2.2:17334589-17334608 None:intergenic 40.0%
! TGGCTTGGAGGAATTTGATA+AGG - chr2.2:17336215-17336234 None:intergenic 40.0%
! TTAGCTCTCTCCATCACATA+AGG - chr2.2:17334991-17335010 None:intergenic 40.0%
!! AAACGCGGTTACTTGTTGTA+TGG + chr2.2:17335210-17335229 MS.gene060604:CDS 40.0%
!! GAGTGTTGAAACTCATTGGA+TGG - chr2.2:17335095-17335114 None:intergenic 40.0%
!! GCAAGAGTGTTGAAACTCAT+TGG - chr2.2:17335099-17335118 None:intergenic 40.0%
AGTTCGCATTAGCGCAATTG+AGG - chr2.2:17334548-17334567 None:intergenic 45.0%
ATAGAAGAACCGGGAAAGCT+TGG + chr2.2:17335187-17335206 MS.gene060604:CDS 45.0%
CCAAGCCAAGAAAATGATCC+AGG + chr2.2:17336227-17336246 MS.gene060604:CDS 45.0%
CTTCTGCTGCCTCAAAAACT+TGG - chr2.2:17334696-17334715 None:intergenic 45.0%
TGGTGAATTGCGCGTTGAAT+TGG - chr2.2:17334639-17334658 None:intergenic 45.0%
TGTACATACGACGAGGTCAT+AGG + chr2.2:17335731-17335750 MS.gene060604:intron 45.0%
! CCTGGATCATTTTCTTGGCT+TGG - chr2.2:17336230-17336249 None:intergenic 45.0%
!! CAAGCCAGTTTTATCTGCAG+TGG + chr2.2:17334522-17334541 MS.gene060604:CDS 45.0%
!! TACTTGTTGTATGGTCCTCC+TGG + chr2.2:17335219-17335238 MS.gene060604:CDS 45.0%
!! TCTTATTTCTGACACAGCCG+AGG - chr2.2:17334911-17334930 None:intergenic 45.0%
!!! GGATCATTTTCTTGGCTTGG+AGG - chr2.2:17336227-17336246 None:intergenic 45.0%
AACATGACTTCTACCGCACG+AGG + chr2.2:17335612-17335631 MS.gene060604:intron 50.0%
AAGCTAGACTTCCCAGTACC+AGG - chr2.2:17335240-17335259 None:intergenic 50.0%
CTACCAGGTCTTAACAGAGC+AGG - chr2.2:17336000-17336019 None:intergenic 50.0%
GAGGCCACTGCAGATAAAAC+TGG - chr2.2:17334529-17334548 None:intergenic 50.0%
GATCCTGCTCTGTTAAGACC+TGG + chr2.2:17335994-17336013 MS.gene060604:CDS 50.0%
GATGGTCTTTGGTCATGCTG+TGG + chr2.2:17335925-17335944 MS.gene060604:CDS 50.0%
TCTTTGGTCATGCTGTGGAG+AGG + chr2.2:17335930-17335949 MS.gene060604:CDS 50.0%
TGTATGGTCCTCCTGGTACT+GGG + chr2.2:17335226-17335245 MS.gene060604:CDS 50.0%
TTGTATGGTCCTCCTGGTAC+TGG + chr2.2:17335225-17335244 MS.gene060604:CDS 50.0%
! GTCTAGCTTGATTGCAGCCA+TGG + chr2.2:17335251-17335270 MS.gene060604:CDS 50.0%
ACCGCGTTTCCAAGCTTTCC+CGG - chr2.2:17335199-17335218 None:intergenic 55.0%
ACCGGGAAAGCTTGGAAACG+CGG + chr2.2:17335195-17335214 MS.gene060604:CDS 55.0%
CTAGACTTCCCAGTACCAGG+AGG - chr2.2:17335237-17335256 None:intergenic 55.0%
GAGGCAGCAGAAGCCTATCT+AGG + chr2.2:17334703-17334722 MS.gene060604:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 17334490 17336323 17334490 ID=MS.gene060604
chr2.2 mRNA 17334490 17336323 17334490 ID=MS.gene060604.t1;Parent=MS.gene060604
chr2.2 exon 17334490 17335458 17334490 ID=MS.gene060604.t1.exon1;Parent=MS.gene060604.t1
chr2.2 CDS 17334490 17335458 17334490 ID=cds.MS.gene060604.t1;Parent=MS.gene060604.t1
chr2.2 exon 17335895 17336323 17335895 ID=MS.gene060604.t1.exon2;Parent=MS.gene060604.t1
chr2.2 CDS 17335895 17336323 17335895 ID=cds.MS.gene060604.t1;Parent=MS.gene060604.t1
Gene Sequence

>MS.gene060604

ATGACAATGATCTCAAAAATGTTTGATAGCAGCAAGCCAGTTTTATCTGCAGTGGCCTCAATTGCGCTAATGCGAACTGTCACAAATGAACTCATTCCTCGTGAAGTCCTAAACATTGTTCAATCGGGACTTCACCATCTTTTTTGCCAATTCAACGCGCAATTCACCATAGTCATTGAAGAATTTCAAGGGATGACAAGAAACCAAGTTTTTGAGGCAGCAGAAGCCTATCTAGGAACTAAAGCAACTGTCTCAGCAGAAAGAGTTAAAGCAACCAAATCTAAGGAACATAAAAAGCTTTCTTTCAATCTAGATAGAGATGAAGAAGTGAGTGATGTTTTTGAAGGTGTTAGTGTAAAGTGGAAACTAATATGCATACAAGTCGACTCATCAAGAATTCGACATTATGATAGGGACTCCTCGGCTGTGTCAGAAATAAGATCATATGAGCTAACTTTTCACAAGAAACACAAAAACAAGATCTTTGATTCATATTTGCCTTATGTGATGGAGAGAGCTAAACAAATTAAACAAGGAATTGTGGCACTTAAGATTCACTCAAATGAATATAATCGTTGGTGTCATGATCCTATTAAGTTCAACCATCCAATGAGTTTCAACACTCTTGCAATTGATGAAGAGCTTCAAAGGGAAATTAAGAATGATTTGGACAAATTTGTGAGTGCTAAAGAATTTTATAGAAGAACCGGGAAAGCTTGGAAACGCGGTTACTTGTTGTATGGTCCTCCTGGTACTGGGAAGTCTAGCTTGATTGCAGCCATGGCTAACTATCTCAACTATGATATCTATGACTTGGATCTCACTAATGTTGGGGATAATAAGAGCTTAAAGCAACTTATTCTCAGCATGTCCAACCGTTCTATACTTGTGATAGAGGATATTGATTGCTCCGTAAAATTGCAAAACCGAGAAGAAGATGAAGAAGTTGTTCATAATGGGCACAATAAGGTATGAATTTCTCTTTGAAAATATGATTCATCTTTGAGTTTCTTTCATACCAAACTGTAATTATTTGAAAGAATTTGACGTACATGAAATCTGGTTTAGATCTATTTAATCATCTGCATGATTTAGCCGGTGAATTTTGGTCCTATTGTTAACAACATGACTTCTACCGCACGAGGACATAATATTTAGCTGAAAAAATTTGGTCTTGTTGGTAAGATTTTGTCGACAATTGTTGGTCAAGTTTGTAAAATATGACATGTATTTTCAAACTATGTACATACGACGAGGTCATAGGTTCGAAATACTTACATATGTAAGTACGCTTTGATTTATGATGTCTGTTCATATCATGGGGACTAATTGAAAGCAATACCAACATATTCTTTTGTTCCTGTCAACTATTTTAAACTAATGTTTGTTGATACTTTTTCAACAGATGACACTTTCGGGACTATTGAATGCAGTAGATGGTCTTTGGTCATGCTGTGGAGAGGAACACATAATTGTTTTCACAACAAATCACAGAGACAGGCTTGATCCTGCTCTGTTAAGACCTGGTAGAATGGACAAGCAAATTCACTTGTCATATTGCAACTTTTCTGCTTTTAAGAAATTGGTTATCAACTACCTTTGCATTACTGAACATGAACTCTTTGAAAAGATTGAAGTTCTTCTTGGACAAGTTCAAGTTACTCCAGCTGAAATTGCAGAAGAGTTAACAAAGGATGTTGATGCTACAGAATGCCTGCAAGACCTTATCAAATTCCTCCAAGCCAAGAAAATGATCCAGGAAGAAACTAAGAGAGAAGAAAATAGCAAAGAAGAACAAGATCTAGGCAGAGAAAATATAGGTCTATCTAAGTAG
Protein sequence

>MS.gene060604.t1

MTMISKMFDSSKPVLSAVASIALMRTVTNELIPREVLNIVQSGLHHLFCQFNAQFTIVIEEFQGMTRNQVFEAAEAYLGTKATVSAERVKATKSKEHKKLSFNLDRDEEVSDVFEGVSVKWKLICIQVDSSRIRHYDRDSSAVSEIRSYELTFHKKHKNKIFDSYLPYVMERAKQIKQGIVALKIHSNEYNRWCHDPIKFNHPMSFNTLAIDEELQREIKNDLDKFVSAKEFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYLNYDIYDLDLTNVGDNKSLKQLILSMSNRSILVIEDIDCSVKLQNREEDEEVVHNGHNKMTLSGLLNAVDGLWSCCGEEHIIVFTTNHRDRLDPALLRPGRMDKQIHLSYCNFSAFKKLVINYLCITEHELFEKIEVLLGQVQVTPAEIAEELTKDVDATECLQDLIKFLQAKKMIQEETKREENSKEEQDLGRENIGLSK