AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64170.t1 MTR_5g029990 98.658 298 4 0 1 298 1 298 0.0 620
MS.gene64170.t1 MTR_6g406250 31.373 306 186 8 6 293 49 348 2.34e-49 167
MS.gene64170.t1 MTR_2g104960 31.475 305 190 9 4 296 12 309 7.68e-38 137
MS.gene64170.t1 MTR_3g005210 28.990 307 194 9 7 296 1 300 3.42e-36 132
MS.gene64170.t1 MTR_5g072620 30.392 306 187 8 9 296 2 299 1.32e-35 130
MS.gene64170.t1 MTR_3g005170 27.723 303 197 7 10 296 8 304 1.48e-34 128
MS.gene64170.t1 MTR_4g009040 31.169 308 172 10 12 296 13 303 1.71e-34 127
MS.gene64170.t1 MTR_4g009040 30.449 312 173 10 12 296 13 307 4.42e-34 127
MS.gene64170.t1 MTR_3g005170 28.520 277 177 6 10 271 8 278 8.12e-34 125
MS.gene64170.t1 MTR_4g006940 31.410 312 189 10 3 296 2 306 9.99e-34 126
MS.gene64170.t1 MTR_8g062440 31.046 306 180 10 11 296 7 301 4.89e-33 124
MS.gene64170.t1 MTR_8g062440 30.263 304 188 9 10 296 6 302 8.84e-33 123
MS.gene64170.t1 MTR_8g062110 28.763 299 194 6 12 296 9 302 3.35e-32 122
MS.gene64170.t1 MTR_8g062110 28.763 299 194 6 12 296 18 311 3.58e-32 122
MS.gene64170.t1 MTR_2g101330 30.451 266 169 5 10 260 3 267 4.12e-32 121
MS.gene64170.t1 MTR_3g031650 28.990 307 190 9 9 296 2 299 4.37e-32 121
MS.gene64170.t1 MTR_8g062440 31.359 287 173 8 11 280 7 286 4.85e-32 121
MS.gene64170.t1 MTR_1g022445 29.747 316 180 11 9 297 5 305 1.02e-31 120
MS.gene64170.t1 MTR_4g006940 33.205 259 149 9 3 243 2 254 3.40e-31 118
MS.gene64170.t1 MTR_4g006940 33.205 259 149 9 3 243 2 254 3.40e-31 118
MS.gene64170.t1 MTR_1g022440 30.216 278 161 10 9 263 5 272 4.66e-31 119
MS.gene64170.t1 MTR_3g031650 29.670 273 167 7 9 263 2 267 7.65e-31 117
MS.gene64170.t1 MTR_8g062110 28.731 268 173 5 11 264 8 271 8.52e-31 117
MS.gene64170.t1 MTR_8g062110 28.731 268 173 5 11 264 8 271 1.06e-30 117
MS.gene64170.t1 MTR_6g406250 31.455 213 134 5 6 206 49 261 1.69e-30 115
MS.gene64170.t1 MTR_4g077130 27.687 307 188 10 12 296 9 303 1.85e-29 114
MS.gene64170.t1 MTR_4g077100 27.152 302 196 8 12 296 11 305 2.15e-29 114
MS.gene64170.t1 MTR_5g056600 26.829 246 162 6 13 243 5 247 2.29e-29 114
MS.gene64170.t1 MTR_4g077100 28.063 253 162 5 12 248 11 259 3.34e-29 112
MS.gene64170.t1 MTR_3g005170 27.852 298 172 9 10 296 8 273 2.50e-27 108
MS.gene64170.t1 MTR_4g009690 31.274 259 152 6 12 252 13 263 5.33e-27 107
MS.gene64170.t1 MTR_4g009690 30.769 260 153 6 12 252 13 264 8.58e-27 107
MS.gene64170.t1 MTR_7g074880 28.253 269 167 7 12 260 8 270 1.15e-26 107
MS.gene64170.t1 MTR_7g074850 28.417 278 163 10 12 263 8 275 1.10e-24 101
MS.gene64170.t1 MTR_7g074820 28.897 263 160 9 12 252 8 265 1.96e-24 100
MS.gene64170.t1 MTR_7g074730 27.306 271 174 8 12 263 8 274 2.95e-24 100
MS.gene64170.t1 MTR_4g081440 28.115 313 184 13 13 292 25 329 1.51e-23 99.0
MS.gene64170.t1 MTR_7g074710 27.820 266 172 5 12 260 8 270 1.86e-23 98.2
MS.gene64170.t1 MTR_4g081440 27.796 313 184 13 13 292 21 324 6.04e-23 97.4
MS.gene64170.t1 MTR_2g028620 29.572 257 153 9 12 247 10 259 6.46e-23 97.1
MS.gene64170.t1 MTR_2g028620 29.572 257 153 9 12 247 10 259 1.24e-22 95.9
MS.gene64170.t1 MTR_8g062110 29.327 208 133 4 98 296 14 216 1.70e-20 88.6
MS.gene64170.t1 MTR_4g023730 31.461 178 107 5 11 177 7 180 1.27e-19 87.8
MS.gene64170.t1 MTR_8g062110 28.889 180 115 3 94 264 10 185 4.16e-19 83.6
MS.gene64170.t1 MTR_7g074870 26.667 225 150 4 48 260 11 232 1.97e-18 84.0
MS.gene64170.t1 MTR_2g013250 22.826 276 178 7 12 254 7 280 1.80e-14 73.2
MS.gene64170.t1 MTR_2g013230 25.294 170 117 5 12 171 8 177 2.34e-11 63.9
MS.gene64170.t1 MTR_7g117190 25.676 148 104 3 12 153 7 154 2.71e-11 63.5
MS.gene64170.t1 MTR_7g117190 25.676 148 104 3 12 153 7 154 3.42e-11 63.5
MS.gene64170.t1 MTR_2g013260 24.118 170 119 4 12 171 8 177 5.55e-11 62.4
MS.gene64170.t1 MTR_2g013260 24.118 170 119 4 12 171 8 177 7.81e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64170.t1 AT2G23910 44.816 299 146 7 8 293 6 298 6.48e-87 262
MS.gene64170.t1 AT4G30470 43.667 300 151 6 6 293 4 297 2.92e-84 256
MS.gene64170.t1 AT2G23910 46.617 266 128 5 8 263 6 267 8.88e-83 251
MS.gene64170.t1 AT2G23910 46.617 266 128 5 8 263 6 267 9.67e-83 251
MS.gene64170.t1 AT2G23910 44.574 258 128 6 45 293 13 264 1.65e-73 227
MS.gene64170.t1 AT5G14700 30.323 310 193 8 6 293 49 357 1.40e-40 145
MS.gene64170.t1 AT5G58490 30.592 304 185 7 11 296 8 303 2.54e-37 135
MS.gene64170.t1 AT1G51410 31.353 303 184 8 11 296 8 303 3.14e-37 135
MS.gene64170.t1 AT2G02400 31.104 299 176 10 7 281 1 293 3.17e-35 129
MS.gene64170.t1 AT1G09510 31.783 258 154 7 12 252 8 260 2.56e-34 127
MS.gene64170.t1 AT5G19440 29.801 302 188 8 12 296 10 304 2.73e-34 127
MS.gene64170.t1 AT4G35420 27.687 307 191 7 5 297 1 290 1.42e-33 125
MS.gene64170.t1 AT1G15950 30.201 298 189 8 11 296 12 302 6.48e-32 121
MS.gene64170.t1 AT1G15950 30.201 298 189 8 11 296 12 302 6.48e-32 121
MS.gene64170.t1 AT1G09480 28.525 305 188 8 12 296 8 302 6.84e-32 120
MS.gene64170.t1 AT1G15950 30.201 298 189 8 11 296 12 302 6.87e-32 121
MS.gene64170.t1 AT1G80820 30.592 304 180 9 12 297 8 298 7.38e-32 121
MS.gene64170.t1 AT1G09480 28.525 305 188 8 12 296 55 349 1.49e-31 121
MS.gene64170.t1 AT1G15950 31.615 291 177 10 11 289 12 292 4.84e-31 118
MS.gene64170.t1 AT1G80820 33.735 249 135 8 12 242 8 244 9.85e-31 116
MS.gene64170.t1 AT2G33600 30.619 307 178 8 7 292 4 296 2.17e-30 117
MS.gene64170.t1 AT1G15950 31.698 265 162 8 11 263 12 269 2.48e-30 115
MS.gene64170.t1 AT1G15950 31.698 265 162 8 11 263 12 269 2.85e-30 115
MS.gene64170.t1 AT2G33590 29.450 309 181 9 6 292 3 296 3.14e-30 116
MS.gene64170.t1 AT1G09490 29.085 306 189 9 10 296 6 302 1.24e-29 115
MS.gene64170.t1 AT1G66800 30.483 269 160 7 12 263 8 266 1.41e-28 112
MS.gene64170.t1 AT1G68540 27.236 246 161 6 14 243 6 249 1.83e-27 109
MS.gene64170.t1 AT5G42800 31.538 260 146 9 10 246 6 256 2.34e-27 110
MS.gene64170.t1 AT4G27250 25.723 311 201 9 11 297 12 316 4.25e-25 103
MS.gene64170.t1 AT1G25460 27.132 258 164 9 25 263 17 269 5.21e-25 102
MS.gene64170.t1 AT4G27250 32.105 190 116 5 11 187 12 201 9.79e-25 102
MS.gene64170.t1 AT1G09500 28.016 257 166 5 12 252 8 261 4.43e-24 99.0
MS.gene64170.t1 AT1G09500 28.016 257 166 5 12 252 8 261 1.11e-23 99.0
MS.gene64170.t1 AT5G14700 29.493 217 137 5 6 206 49 265 1.68e-23 97.8
MS.gene64170.t1 AT1G09490 30.088 226 135 7 56 266 25 242 4.31e-22 94.0
MS.gene64170.t1 AT1G61720 27.099 262 159 7 9 243 10 266 9.12e-22 94.0
MS.gene64170.t1 AT1G76470 27.362 307 194 10 10 296 5 302 3.36e-20 89.4
MS.gene64170.t1 AT2G45400 25.749 334 189 11 3 297 31 344 2.31e-18 84.7
MS.gene64170.t1 AT2G45400 26.545 275 157 7 2 243 30 292 8.31e-18 82.4
MS.gene64170.t1 AT1G68540 27.632 152 100 3 110 252 1 151 1.15e-17 80.1
MS.gene64170.t1 AT2G45400 28.505 214 137 4 3 202 31 242 2.02e-17 80.5
MS.gene64170.t1 AT1G09500 27.273 209 137 4 56 252 22 227 5.77e-16 77.0
MS.gene64170.t1 AT1G15950 28.986 207 136 5 98 296 1 204 3.45e-15 73.9

Find 58 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAAGTTGAACAAATTAA+TGG 0.319570 5.2:+18817974 MS.gene64170:CDS
GTCTTTGTGACCGTTGATTT+AGG 0.322077 5.2:+18817591 MS.gene64170:CDS
AGCATAATTGATGCTCTTAG+AGG 0.347208 5.2:+18815402 MS.gene64170:CDS
CATGCATCCATTCAACAATA+TGG 0.361898 5.2:+18815126 MS.gene64170:CDS
TAAGACCATAGAACCTGATT+TGG 0.363147 5.2:+18816702 MS.gene64170:CDS
AATGGACAGAGGAGTGAATA+TGG 0.373451 5.2:+18817479 MS.gene64170:CDS
TTCATCCAAATCAGGTTCTA+TGG 0.374831 5.2:-18816707 None:intergenic
CAATGTATTTCATCCAAATC+AGG 0.379342 5.2:-18816715 None:intergenic
CGGTAATGAACAGGAATATA+TGG 0.382600 5.2:+18816564 MS.gene64170:intron
CAAATTAATGGTGGACTTTG+AGG 0.389339 5.2:+18817986 MS.gene64170:CDS
GTTTGTGTCATGGATGCTTC+AGG 0.401929 5.2:+18815051 MS.gene64170:CDS
CCTCCGCAACTGCTGTGGTT+TGG 0.409242 5.2:+18816668 MS.gene64170:CDS
ATGGATGCTTCAGGTCAATT+AGG 0.424212 5.2:+18815060 MS.gene64170:CDS
CCGCAACTGCTGTGGTTTGG+AGG 0.433210 5.2:+18816671 MS.gene64170:CDS
TCAGCAGCTCCTCTCAAGTA+AGG 0.433540 5.2:-18817552 None:intergenic
GATGCTCTTAGAGGTTGCTC+TGG 0.436699 5.2:+18815411 MS.gene64170:CDS
ATTATGCTATGATAATCAAA+AGG 0.437929 5.2:-18815387 None:intergenic
TCATAATTGGGCTGGTCAAA+GGG 0.439042 5.2:-18815456 None:intergenic
ATCATAATTGGGCTGGTCAA+AGG 0.444436 5.2:-18815457 None:intergenic
TGCTTACATCATAATTGGGC+TGG 0.462300 5.2:-18815464 None:intergenic
CAATTTCTGTCGCAAATTCA+AGG 0.478165 5.2:+18816747 MS.gene64170:CDS
ATGTTGTGCAGTGATGACTA+TGG 0.478181 5.2:+18817921 MS.gene64170:intron
GCAGGAGAAGATCCATTCAA+TGG 0.492642 5.2:+18815321 MS.gene64170:intron
TCAGCTGAAATCCCATTGAA+TGG 0.493043 5.2:-18815333 None:intergenic
CGCAACTGCTGTGGTTTGGA+GGG 0.495829 5.2:+18816672 MS.gene64170:CDS
ATAATTCAGTCATACCTTTG+TGG 0.497881 5.2:-18817756 None:intergenic
TCAGTCATACCTTTGTGGTA+AGG 0.505180 5.2:-18817751 None:intergenic
GTTCAAAGACTTCTTCAAAG+AGG 0.514233 5.2:+18815093 MS.gene64170:CDS
CACATCCTCCGCAACTGCTG+TGG 0.524456 5.2:+18816663 MS.gene64170:CDS
CACTCCTCTGTCCATTGCTA+AGG 0.525382 5.2:-18817472 None:intergenic
CCTCCAAACCACAGCAGTTG+CGG 0.528661 5.2:-18816671 None:intergenic
CAGGAGAAGATCCATTCAAT+GGG 0.530786 5.2:+18815322 MS.gene64170:intron
TTATGCTATGATAATCAAAA+GGG 0.531153 5.2:-18815386 None:intergenic
AATATGGTATCAATCAATGC+TGG 0.538235 5.2:+18817495 MS.gene64170:CDS
TGACTAGTTATGTCAAAAGA+AGG 0.541260 5.2:-18815024 None:intergenic
ATATGTACCATATTGTTGAA+TGG 0.548967 5.2:-18815133 None:intergenic
CTGATTTGGATGAAATACAT+TGG 0.555033 5.2:+18816716 MS.gene64170:CDS
ACAAACAAACAGTTATGGCA+TGG 0.567066 5.2:+18817414 MS.gene64170:intron
TACGAGGATGTCTCATCGTA+CGG 0.580649 5.2:+18817642 MS.gene64170:CDS
AACAGCGTTTGCCTTAGCAA+TGG 0.587609 5.2:+18817461 MS.gene64170:CDS
ATCAAACACCCTTACTTGAG+AGG 0.597030 5.2:+18817543 MS.gene64170:CDS
CAGCAGCTCCTCTCAAGTAA+GGG 0.598914 5.2:-18817551 None:intergenic
TTTGAAGAAGTCTTTGAACA+AGG 0.606201 5.2:-18815089 None:intergenic
CAAACAAACAGTTATGGCAT+GGG 0.606327 5.2:+18817415 MS.gene64170:intron
TAGTCATACTGTTTGTGTCA+TGG 0.607789 5.2:+18815041 MS.gene64170:CDS
GAAGTTGAACAAATTAATGG+TGG 0.616912 5.2:+18817977 MS.gene64170:CDS
TAACTTATGTGCAAGCTGAA+CGG 0.619871 5.2:-18817709 None:intergenic
TTGTGGTAAGGAAGAAGATG+TGG 0.620890 5.2:-18817739 None:intergenic
CCAAACCACAGCAGTTGCGG+AGG 0.635300 5.2:-18816668 None:intergenic
TTGTTGAATGGATGCATGAA+CGG 0.636150 5.2:-18815121 None:intergenic
CATCTTCTTCCTTACCACAA+AGG 0.639609 5.2:+18817742 MS.gene64170:CDS
TTTGCCTTAGCAATGGACAG+AGG 0.681382 5.2:+18817468 MS.gene64170:CDS
TACTCACATCTGTGTCTACG+AGG 0.683844 5.2:+18817626 MS.gene64170:CDS
GCTGCTGAAATGTACGAGGA+TGG 0.684788 5.2:+18817567 MS.gene64170:CDS
ACAGGAATATATGGCTGACG+TGG 0.685182 5.2:+18816573 MS.gene64170:intron
TAATTGGGCTGGTCAAAGGG+AGG 0.685897 5.2:-18815453 None:intergenic
GGTAAGGAAGAAGATGTGGT+AGG 0.716806 5.2:-18817735 None:intergenic
AGGAGCTGCTGAAATGTACG+AGG 0.764112 5.2:+18817563 MS.gene64170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGATTAAAAAGTTAAAA+AGG - chr5.2:18815503-18815522 None:intergenic 10.0%
!! AAAGATTAAAAAGTTAAAAA+GGG - chr5.2:18815502-18815521 None:intergenic 10.0%
!!! CATTTTTGTTTAAATTTAAT+AGG + chr5.2:18816967-18816986 MS.gene64170:intron 10.0%
!! AAAAATTGTAACATAAACAT+TGG - chr5.2:18815642-18815661 None:intergenic 15.0%
!! GAAAAAATTATTTAGATTGT+GGG + chr5.2:18816888-18816907 MS.gene64170:intron 15.0%
!! TGAAAAAATTATTTAGATTG+TGG + chr5.2:18816887-18816906 MS.gene64170:intron 15.0%
!!! TAACTTTTTCTATATTCAAT+AGG - chr5.2:18817377-18817396 None:intergenic 15.0%
!!! TCAAACATTTTAACTTATAA+AGG + chr5.2:18817783-18817802 MS.gene64170:intron 15.0%
!!! TTTATTTGATTTTGACTATA+TGG + chr5.2:18817867-18817886 MS.gene64170:intron 15.0%
!! AAAAACAAATACTTCTACAT+GGG - chr5.2:18816489-18816508 None:intergenic 20.0%
!! AAGCACTAATACAAATTTAA+AGG - chr5.2:18815866-18815885 None:intergenic 20.0%
!! ATTATGCTATGATAATCAAA+AGG - chr5.2:18815390-18815409 None:intergenic 20.0%
!! ATTCATCTTAAATGAATAAG+TGG + chr5.2:18815188-18815207 MS.gene64170:intron 20.0%
!! CTAAGCAAATTATTGTAATT+TGG + chr5.2:18816039-18816058 MS.gene64170:intron 20.0%
!! GATCAAATTTCATAGAAATT+TGG + chr5.2:18815695-18815714 MS.gene64170:intron 20.0%
!! TAAAAACAAATACTTCTACA+TGG - chr5.2:18816490-18816509 None:intergenic 20.0%
!! TATACTTTAAACATTGTGTT+TGG + chr5.2:18816783-18816802 MS.gene64170:intron 20.0%
!! TTAAATGACTAAGACATATT+TGG - chr5.2:18816122-18816141 None:intergenic 20.0%
!! TTATGCTATGATAATCAAAA+GGG - chr5.2:18815389-18815408 None:intergenic 20.0%
!!! ATAGGTTAAAGTTTGAAATT+TGG + chr5.2:18816985-18817004 MS.gene64170:intron 20.0%
!!! TATCAAAAGACACAATTTTA+TGG - chr5.2:18817126-18817145 None:intergenic 20.0%
!!! TTTCATAGAAATTTGGATTT+TGG + chr5.2:18815702-18815721 MS.gene64170:intron 20.0%
!!! TTTTTGCTTACATCATAATT+GGG - chr5.2:18815471-18815490 None:intergenic 20.0%
! AATGTACAAACAAACAGTTA+TGG + chr5.2:18817409-18817428 MS.gene64170:intron 25.0%
! ATATGTACCATATTGTTGAA+TGG - chr5.2:18815136-18815155 None:intergenic 25.0%
! CAAGAAGTTGAACAAATTAA+TGG + chr5.2:18817974-18817993 MS.gene64170:CDS 25.0%
! TAATGATTGAATAGAAGTCT+TGG - chr5.2:18816823-18816842 None:intergenic 25.0%
! TAATTCTAGATAGACTCTTA+AGG - chr5.2:18815617-18815636 None:intergenic 25.0%
! TTGTTACTAATCAATCATGA+TGG + chr5.2:18816245-18816264 MS.gene64170:intron 25.0%
!! AAAGGTATGACTGAATTATA+TGG + chr5.2:18817760-18817779 MS.gene64170:intron 25.0%
!! AGCTCGATTGATAATAATAT+TGG - chr5.2:18815249-18815268 None:intergenic 25.0%
!! CAATGATCCAAATTTTGTTA+TGG + chr5.2:18816303-18816322 MS.gene64170:intron 25.0%
!! CTTTTGATAAGATTCTTATG+TGG + chr5.2:18817137-18817156 MS.gene64170:intron 25.0%
!! GCTCGATTGATAATAATATT+GGG - chr5.2:18815248-18815267 None:intergenic 25.0%
!! GTTTTTGCTTACATCATAAT+TGG - chr5.2:18815472-18815491 None:intergenic 25.0%
!! TAATTGATGATAACATGTGT+CGG + chr5.2:18816544-18816563 MS.gene64170:intron 25.0%
!!! AATAATTTGCTTAGTTGTCA+TGG - chr5.2:18816033-18816052 None:intergenic 25.0%
!!! CTTATAAAGGTGTTCAAATA+AGG + chr5.2:18817796-18817815 MS.gene64170:intron 25.0%
!!! GACTCATTCATTTAGTTTTA+AGG + chr5.2:18817840-18817859 MS.gene64170:intron 25.0%
!!! GTTCAAATAAGGTTGTTTTA+TGG + chr5.2:18817807-18817826 MS.gene64170:intron 25.0%
AAGAGTTGCTACAAAAACAT+AGG + chr5.2:18816392-18816411 MS.gene64170:intron 30.0%
AATATGGTATCAATCAATGC+TGG + chr5.2:18817495-18817514 MS.gene64170:CDS 30.0%
AGAAGAAACTAGAGTTAAGA+GGG - chr5.2:18815971-18815990 None:intergenic 30.0%
ATAATTCAGTCATACCTTTG+TGG - chr5.2:18817759-18817778 None:intergenic 30.0%
CAAGTTGCCATAACAAAATT+TGG - chr5.2:18816313-18816332 None:intergenic 30.0%
CAATGTATTTCATCCAAATC+AGG - chr5.2:18816718-18816737 None:intergenic 30.0%
CACCAAAAATCCTAAATCAA+CGG - chr5.2:18817604-18817623 None:intergenic 30.0%
CATCAGAATCATCTTATCAA+AGG - chr5.2:18817191-18817210 None:intergenic 30.0%
GAAGTTGAACAAATTAATGG+TGG + chr5.2:18817977-18817996 MS.gene64170:CDS 30.0%
TAAAATTGTATGACTGCATG+CGG + chr5.2:18817302-18817321 MS.gene64170:intron 30.0%
TAGAAGAAACTAGAGTTAAG+AGG - chr5.2:18815972-18815991 None:intergenic 30.0%
TATGTCTTAGTCATTTAAGC+TGG + chr5.2:18816124-18816143 MS.gene64170:intron 30.0%
TCAGAATCATCTTATCTGAT+CGG - chr5.2:18817085-18817104 None:intergenic 30.0%
TTAGAATCATCTGATCTGAT+CGG - chr5.2:18817255-18817274 None:intergenic 30.0%
TTGATAAGATGATTCTGATG+TGG + chr5.2:18817191-18817210 MS.gene64170:intron 30.0%
TTTGAAGAAGTCTTTGAACA+AGG - chr5.2:18815092-18815111 None:intergenic 30.0%
! TGACTAGTTATGTCAAAAGA+AGG - chr5.2:18815027-18815046 None:intergenic 30.0%
!! CTGATTTGGATGAAATACAT+TGG + chr5.2:18816716-18816735 MS.gene64170:CDS 30.0%
!!! TTTTGGAAGTACTATCTTCT+TGG + chr5.2:18815719-18815738 MS.gene64170:intron 30.0%
ACAAACAAACAGTTATGGCA+TGG + chr5.2:18817414-18817433 MS.gene64170:intron 35.0%
AGCAAGCAAACACCATTTAT+TGG + chr5.2:18815900-18815919 MS.gene64170:intron 35.0%
AGCATAATTGATGCTCTTAG+AGG + chr5.2:18815402-18815421 MS.gene64170:CDS 35.0%
ATCAATCGAGCTAAGTTCAT+TGG + chr5.2:18815257-18815276 MS.gene64170:intron 35.0%
CAAACAAACAGTTATGGCAT+GGG + chr5.2:18817415-18817434 MS.gene64170:intron 35.0%
CAATTTCTGTCGCAAATTCA+AGG + chr5.2:18816747-18816766 MS.gene64170:CDS 35.0%
CAGATAAGATGATTCTGATG+TGG + chr5.2:18817087-18817106 MS.gene64170:intron 35.0%
CAGATCAGATGATTCTAATG+TGG + chr5.2:18817257-18817276 MS.gene64170:intron 35.0%
CATGCATCCATTCAACAATA+TGG + chr5.2:18815126-18815145 MS.gene64170:CDS 35.0%
CGGTAATGAACAGGAATATA+TGG + chr5.2:18816564-18816583 MS.gene64170:intron 35.0%
CTGTTGATATTGTTCTGCTT+TGG + chr5.2:18815294-18815313 MS.gene64170:intron 35.0%
GACTAAGACATATTTGGTCA+AGG - chr5.2:18816116-18816135 None:intergenic 35.0%
GTTCAAAGACTTCTTCAAAG+AGG + chr5.2:18815093-18815112 MS.gene64170:CDS 35.0%
TAACTTATGTGCAAGCTGAA+CGG - chr5.2:18817712-18817731 None:intergenic 35.0%
TAAGACCATAGAACCTGATT+TGG + chr5.2:18816702-18816721 MS.gene64170:CDS 35.0%
TAGTCATACTGTTTGTGTCA+TGG + chr5.2:18815041-18815060 MS.gene64170:CDS 35.0%
TCAATCGAGCTAAGTTCATT+GGG + chr5.2:18815258-18815277 MS.gene64170:intron 35.0%
TTCATCCAAATCAGGTTCTA+TGG - chr5.2:18816710-18816729 None:intergenic 35.0%
TTGATCAGATGATTCTGATG+TGG + chr5.2:18817021-18817040 MS.gene64170:intron 35.0%
TTGTTGAATGGATGCATGAA+CGG - chr5.2:18815124-18815143 None:intergenic 35.0%
TTTGATATTTGCACGCAATG+TGG + chr5.2:18816272-18816291 MS.gene64170:intron 35.0%
! ACTCTAGTTTCTTCTACTTC+TGG + chr5.2:18815976-18815995 MS.gene64170:intron 35.0%
! CTCTAGTTTCTTCTACTTCT+GGG + chr5.2:18815977-18815996 MS.gene64170:intron 35.0%
!! CAAATTAATGGTGGACTTTG+AGG + chr5.2:18817986-18818005 MS.gene64170:CDS 35.0%
!!! CGTTGATTTAGGATTTTTGG+TGG + chr5.2:18817602-18817621 MS.gene64170:CDS 35.0%
!!! GACCGTTGATTTAGGATTTT+TGG + chr5.2:18817599-18817618 MS.gene64170:CDS 35.0%
!!! TGCAGTGTTTTAGTCAACTA+TGG - chr5.2:18816513-18816532 None:intergenic 35.0%
AACAAGCAAGTGAGTAACTC+AGG - chr5.2:18815948-18815967 None:intergenic 40.0%
AAGTGAGTAACTCAGGCAAA+TGG - chr5.2:18815941-18815960 None:intergenic 40.0%
AATGGACAGAGGAGTGAATA+TGG + chr5.2:18817479-18817498 MS.gene64170:CDS 40.0%
AGAATTCGAGATTCGAACTC+TGG + chr5.2:18815211-18815230 MS.gene64170:intron 40.0%
ATCAAACACCCTTACTTGAG+AGG + chr5.2:18817543-18817562 MS.gene64170:CDS 40.0%
ATGGATGCTTCAGGTCAATT+AGG + chr5.2:18815060-18815079 MS.gene64170:CDS 40.0%
ATGTTGTGCAGTGATGACTA+TGG + chr5.2:18817921-18817940 MS.gene64170:intron 40.0%
CAGATGATTCTAATGTGGTG+TGG + chr5.2:18817262-18817281 MS.gene64170:intron 40.0%
CAGGAGAAGATCCATTCAAT+GGG + chr5.2:18815322-18815341 MS.gene64170:intron 40.0%
CATCTTCTTCCTTACCACAA+AGG + chr5.2:18817742-18817761 MS.gene64170:CDS 40.0%
GAATAGAAGTCTTGGACAGT+AGG - chr5.2:18816815-18816834 None:intergenic 40.0%
TCAGCTGAAATCCCATTGAA+TGG - chr5.2:18815336-18815355 None:intergenic 40.0%
TCAGTCATACCTTTGTGGTA+AGG - chr5.2:18817754-18817773 None:intergenic 40.0%
TGCTTACATCATAATTGGGC+TGG - chr5.2:18815467-18815486 None:intergenic 40.0%
TTGTGGTAAGGAAGAAGATG+TGG - chr5.2:18817742-18817761 None:intergenic 40.0%
TTGTTCTGCTTTGGTTATGC+AGG + chr5.2:18815303-18815322 MS.gene64170:intron 40.0%
! AACATGTGTCGGTAATGAAC+AGG + chr5.2:18816555-18816574 MS.gene64170:intron 40.0%
!! ATCATAATTGGGCTGGTCAA+AGG - chr5.2:18815460-18815479 None:intergenic 40.0%
!! GTCTTTGTGACCGTTGATTT+AGG + chr5.2:18817591-18817610 MS.gene64170:CDS 40.0%
!! TCATAATTGGGCTGGTCAAA+GGG - chr5.2:18815459-18815478 None:intergenic 40.0%
AACAGCGTTTGCCTTAGCAA+TGG + chr5.2:18817461-18817480 MS.gene64170:CDS 45.0%
ACACCATTTATTGGTGCACG+TGG + chr5.2:18815909-18815928 MS.gene64170:intron 45.0%
ACAGGAATATATGGCTGACG+TGG + chr5.2:18816573-18816592 MS.gene64170:intron 45.0%
CATTTATTGGTGCACGTGGT+AGG + chr5.2:18815913-18815932 MS.gene64170:intron 45.0%
CTACCACGTGCACCAATAAA+TGG - chr5.2:18815915-18815934 None:intergenic 45.0%
GCAGGAGAAGATCCATTCAA+TGG + chr5.2:18815321-18815340 MS.gene64170:intron 45.0%
GGTAAGGAAGAAGATGTGGT+AGG - chr5.2:18817738-18817757 None:intergenic 45.0%
GTTTGTGTCATGGATGCTTC+AGG + chr5.2:18815051-18815070 MS.gene64170:CDS 45.0%
TACGAGGATGTCTCATCGTA+CGG + chr5.2:18817642-18817661 MS.gene64170:CDS 45.0%
TACTCACATCTGTGTCTACG+AGG + chr5.2:18817626-18817645 MS.gene64170:CDS 45.0%
TTGCTTAGTTGTCATGGCCT+TGG - chr5.2:18816027-18816046 None:intergenic 45.0%
TTTGCCTTAGCAATGGACAG+AGG + chr5.2:18817468-18817487 MS.gene64170:CDS 45.0%
! AATACCCTTTTCGTACGCCA+AGG + chr5.2:18816007-18816026 MS.gene64170:intron 45.0%
AGGAGCTGCTGAAATGTACG+AGG + chr5.2:18817563-18817582 MS.gene64170:CDS 50.0%
CACTCCTCTGTCCATTGCTA+AGG - chr5.2:18817475-18817494 None:intergenic 50.0%
CAGCAGCTCCTCTCAAGTAA+GGG - chr5.2:18817554-18817573 None:intergenic 50.0%
CTGATGTGGCTGATTGCATG+CGG + chr5.2:18817035-18817054 MS.gene64170:intron 50.0%
GATGCTCTTAGAGGTTGCTC+TGG + chr5.2:18815411-18815430 MS.gene64170:CDS 50.0%
GCTGCTGAAATGTACGAGGA+TGG + chr5.2:18817567-18817586 MS.gene64170:CDS 50.0%
GTGGTGTGGTTGACTGTATG+CGG + chr5.2:18817276-18817295 MS.gene64170:intron 50.0%
TCAGCAGCTCCTCTCAAGTA+AGG - chr5.2:18817555-18817574 None:intergenic 50.0%
! ATGGCCTTGGCGTACGAAAA+GGG - chr5.2:18816014-18816033 None:intergenic 50.0%
!! TAATTGGGCTGGTCAAAGGG+AGG - chr5.2:18815456-18815475 None:intergenic 50.0%
CCTCCAAACCACAGCAGTTG+CGG - chr5.2:18816674-18816693 None:intergenic 55.0%
! CATGGCCTTGGCGTACGAAA+AGG - chr5.2:18816015-18816034 None:intergenic 55.0%
! CGCAACTGCTGTGGTTTGGA+GGG + chr5.2:18816672-18816691 MS.gene64170:CDS 55.0%
CACATCCTCCGCAACTGCTG+TGG + chr5.2:18816663-18816682 MS.gene64170:CDS 60.0%
CCAAACCACAGCAGTTGCGG+AGG - chr5.2:18816671-18816690 None:intergenic 60.0%
CCTCCGCAACTGCTGTGGTT+TGG + chr5.2:18816668-18816687 MS.gene64170:CDS 60.0%
! CCGCAACTGCTGTGGTTTGG+AGG + chr5.2:18816671-18816690 MS.gene64170:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 18815018 18818013 18815018 ID=MS.gene64170
chr5.2 mRNA 18815018 18818013 18815018 ID=MS.gene64170.t1;Parent=MS.gene64170
chr5.2 exon 18815018 18815147 18815018 ID=MS.gene64170.t1.exon1;Parent=MS.gene64170.t1
chr5.2 CDS 18815018 18815147 18815018 ID=cds.MS.gene64170.t1;Parent=MS.gene64170.t1
chr5.2 exon 18815325 18815479 18815325 ID=MS.gene64170.t1.exon2;Parent=MS.gene64170.t1
chr5.2 CDS 18815325 18815479 18815325 ID=cds.MS.gene64170.t1;Parent=MS.gene64170.t1
chr5.2 exon 18816577 18816768 18816577 ID=MS.gene64170.t1.exon3;Parent=MS.gene64170.t1
chr5.2 CDS 18816577 18816768 18816577 ID=cds.MS.gene64170.t1;Parent=MS.gene64170.t1
chr5.2 exon 18817426 18817763 18817426 ID=MS.gene64170.t1.exon4;Parent=MS.gene64170.t1
chr5.2 CDS 18817426 18817763 18817426 ID=cds.MS.gene64170.t1;Parent=MS.gene64170.t1
chr5.2 exon 18817932 18818013 18817932 ID=MS.gene64170.t1.exon5;Parent=MS.gene64170.t1
chr5.2 CDS 18817932 18818013 18817932 ID=cds.MS.gene64170.t1;Parent=MS.gene64170.t1
Gene Sequence

>MS.gene64170

ATGGCACCTTCTTTTGACATAACTAGTCATACTGTTTGTGTCATGGATGCTTCAGGTCAATTAGGCTTTAGCCTTGTTCAAAGACTTCTTCAAAGAGGATATACCGTTCATGCATCCATTCAACAATATGGTACATATTATGTTATTAAATGTTTTTTTATTGTTGTCAAATTCATCTTAAATGAATAAGTGGAGAATTCGAGATTCGAACTCTGGTCACTGCATATCCCAATATTATTATCAATCGAGCTAAGTTCATTGGGACAATTGATCAGACTGTTGATATTGTTCTGCTTTGGTTATGCAGGAGAAGATCCATTCAATGGGATTTCAGCTGATTCTGATAAATTAAAGATTTTTCGATCAGACCCTTTTGATTATCATAGCATAATTGATGCTCTTAGAGGTTGCTCTGGATTGTTTTACTCATTTGAACCTCCCTTTGACCAGCCCAATTATGATGTAAGCAAAAACAAAAACACCCTTTTTAACTTTTTAATCTTTTAGCTTGTTTTTGCTCTATATTTCTCATATACAAATTTGAGTTAGATAAATTTATTTTATATTATATTAATATTAATCATGCATTATACATGCCTTAAGAGTCTATCTAGAATTAAACCAATGTTTATGTTACAATTTTTTATATTTACATTAAAAAAAGTTTTAAGCATATAGATCAAATTTCATAGAAATTTGGATTTTGGAAGTACTATCTTCTTGGACAAGTCTTGCAATAAAAAATGATATTGATGATGTAAGAATAGTTGAAGAGTTAATGCATGAGTTTTTTTTTGCTGTTGCTAGCTAGCTCTAGCTGTTGTGTGATATGAAAAAGTAGAGTTCCTTTAAATTTGTATTAGTGCTTTATGAAAACAAGCAAGCAAGCAAACACCATTTATTGGTGCACGTGGTAGGCACCATTTGCCTGAGTTACTCACTTGCTTGTTCCCTCTTAACTCTAGTTTCTTCTACTTCTGGGATTGATCAATACCCTTTTCGTACGCCAAGGCCATGACAACTAAGCAAATTATTGTAATTTGGTTTGTACTATAATTGATGATGATGATTTATTTGTTAAAGTTATGTCATAGTCCTTGACCAAATATGTCTTAGTCATTTAAGCTGGTTAATCTTCTAATAACGTGCTTATTTAATTGTATGATAAGTATAAGTGTATGATTATATCAACATTATCTATAAAAATATTAGCAATTATCTATTAAATTGTTACTAATCAATCATGATGGAAATTTTGATATTTGCACGCAATGTGGAACACATGCAATGATCCAAATTTTGTTATGGCAACTTGATAGAATGATAGTATACAATTAATTTTATGTCAATCATAGCATTATTGTGATTAATTTAAAGAGTTGCTACAAAAACATAGGCAAATATTTTATACAGAGAATTTTTTATATGTAAATTAGCATTCTCATATCAGAATCTTTTGTAAGTTCATCCCATGTAGAAGTATTTGTTTTTACCATAGTTGACTAAAACACTGCATTATTGTTTTCTAATTGATGATAACATGTGTCGGTAATGAACAGGAATATATGGCTGACGTGGAAGTTAGAGCAGCACACAACGTGCTTGAAGCTTGTGCTCAAACAGAAACAATAGATAAAGTTGTGTTCACATCCTCCGCAACTGCTGTGGTTTGGAGGGAAGATCGTAAGACCATAGAACCTGATTTGGATGAAATACATTGGAGTGATGTCAATTTCTGTCGCAAATTCAAGGTATATAATAGTACTATACTTTAAACATTGTGTTTGGACTTGTCCTACTGTCCAAGACTTCTATTCAATCATTAAATTGATGATGCACACAATAAATAAAATAAATCTACATGACTCATGAAAAAATTATTTAGATTGTGGGTATACATCTAGATCGTTCGATCAAGATCAAATTATCGAAACATCAATGCTCAATCACATTTTTGTTTAAATTTAATAGGTTAAAGTTTGAAATTTGGATTGTCTGATCTTTTGATCAGATGATTCTGATGTGGCTGATTGCATGCGGTCATAAAATTGTGATTTTGATTGTCCGATCAGATAAGATGATTCTGATGTGGTGACTGCATGCAACCATAAAATTGTGTCTTTTGATAAGATTCTTATGTGGTTGACTGCATGCAGTCATAAAATTTTGTCCTTTGATAAGATGATTCTGATGTGGCTGATTGCATGCAGTCATAAAATTGTGATTTTGATTGTCCGATCAGATCAGATGATTCTAATGTGGTGTGGTTGACTGTATGCGGTCATAAAATTGTATGACTGCATGCGGTCATCGATTGTGACTACACAACTGACAAGCATGAAAAATAGATTGATTACCTATTGAATATAGAAAAAGTTAATAATTGATTGAAATGTACAAACAAACAGTTATGGCATGGGATGTCAAAGACACTAGCAGAGAAAACAGCGTTTGCCTTAGCAATGGACAGAGGAGTGAATATGGTATCAATCAATGCTGGACTATTGATGACACATGATCTCTCTATCAAACACCCTTACTTGAGAGGAGCTGCTGAAATGTACGAGGATGGTGTCTTTGTGACCGTTGATTTAGGATTTTTGGTGGATACTCACATCTGTGTCTACGAGGATGTCTCATCGTACGGTCGTTATTTATGCTTTAATCACATTGTTAATACTCAAGACGACGCCGTTCAGCTTGCACATAAGTTAACACCTACCACATCTTCTTCCTTACCACAAAGGTATGACTGAATTATATGGTCAAACATTTTAACTTATAAAGGTGTTCAAATAAGGTTGTTTTATGGTCAAACATTTGACTCATTCATTTAGTTTTAAGGTTGTTTTATTTGATTTTGACTATATGGACAAATAATTCAACTAAAGTTTTTTTTTTTAATGTTGTGCAGTGATGACTATGGAAAGAGTTACATTGAACAGAAAATAAGCAACAAGAAGTTGAACAAATTAATGGTGGACTTTGAGGCTTAA
Protein sequence

>MS.gene64170.t1

MAPSFDITSHTVCVMDASGQLGFSLVQRLLQRGYTVHASIQQYGEDPFNGISADSDKLKIFRSDPFDYHSIIDALRGCSGLFYSFEPPFDQPNYDEYMADVEVRAAHNVLEACAQTETIDKVVFTSSATAVVWREDRKTIEPDLDEIHWSDVNFCRKFKLWHGMSKTLAEKTAFALAMDRGVNMVSINAGLLMTHDLSIKHPYLRGAAEMYEDGVFVTVDLGFLVDTHICVYEDVSSYGRYLCFNHIVNTQDDAVQLAHKLTPTTSSSLPQSDDYGKSYIEQKISNKKLNKLMVDFEA