AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64175


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64175.t1 MTR_5g030070 98.885 269 3 0 1 269 1 269 0.0 546
MS.gene64175.t1 MTR_8g066790 75.093 269 67 0 1 269 1 269 2.78e-151 423
MS.gene64175.t1 MTR_8g101600 50.373 268 133 0 1 268 1 268 8.19e-100 293
MS.gene64175.t1 MTR_4g061010 45.956 272 141 3 1 266 1 272 2.12e-78 239
MS.gene64175.t1 MTR_4g129670 45.318 267 142 3 1 264 1 266 3.84e-76 232
MS.gene64175.t1 MTR_1g095530 41.445 263 144 3 4 266 5 257 2.09e-66 208
MS.gene64175.t1 MTR_6g033335 41.016 256 141 3 4 259 5 250 2.27e-66 208
MS.gene64175.t1 MTR_4g112570 34.231 260 168 2 3 259 6 265 6.97e-54 176
MS.gene64175.t1 MTR_8g097280 31.984 247 159 4 1 244 3 243 7.28e-49 163
MS.gene64175.t1 MTR_2g056130 31.250 256 159 3 3 256 4 244 5.01e-44 150
MS.gene64175.t1 MTR_5g097460 28.458 253 155 6 5 252 50 281 1.38e-29 114
MS.gene64175.t1 MTR_8g097250 28.866 97 64 2 119 212 28 122 7.32e-12 61.6
MS.gene64175.t1 MTR_5g097450 30.233 129 66 4 118 241 84 193 1.23e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64175.t1 AT5G46700 67.286 269 88 0 1 269 1 269 2.13e-128 365
MS.gene64175.t1 AT2G19580 57.249 269 114 1 1 268 1 269 1.65e-100 295
MS.gene64175.t1 AT3G45600 44.528 265 141 3 1 259 1 265 1.75e-81 247
MS.gene64175.t1 AT4G28050 42.636 258 146 1 4 259 5 262 4.27e-78 237
MS.gene64175.t1 AT4G30430 41.603 262 151 1 3 262 4 265 2.35e-76 233
MS.gene64175.t1 AT2G23810 42.966 263 147 2 4 263 5 267 3.11e-76 233
MS.gene64175.t1 AT5G60220 43.893 262 141 3 1 256 1 262 2.04e-63 202
MS.gene64175.t1 AT1G18520 38.550 262 153 4 3 257 4 264 5.14e-61 194
MS.gene64175.t1 AT3G12090 40.316 253 139 4 4 256 5 245 1.29e-58 188
MS.gene64175.t1 AT4G23410 37.066 259 152 3 3 261 4 251 3.13e-55 179
MS.gene64175.t1 AT1G63260 32.171 258 167 3 1 257 3 253 2.74e-54 176
MS.gene64175.t1 AT1G63260 32.510 243 162 2 1 242 3 244 3.36e-51 169
MS.gene64175.t1 AT1G63260 32.510 243 162 2 1 242 3 244 3.36e-51 169
MS.gene64175.t1 AT1G63260 32.510 243 162 2 1 242 3 244 4.40e-51 169
MS.gene64175.t1 AT1G63260 32.719 217 144 2 1 216 3 218 3.48e-46 154
MS.gene64175.t1 AT3G45600 43.275 171 92 2 1 166 1 171 2.99e-43 146
MS.gene64175.t1 AT2G03840 29.707 239 160 4 19 251 32 268 1.40e-37 134
MS.gene64175.t1 AT1G63260 30.337 178 123 1 1 178 3 179 2.22e-33 121
MS.gene64175.t1 AT5G23030 27.132 258 183 2 1 256 2 256 2.92e-31 117
MS.gene64175.t1 AT5G57810 26.316 133 82 1 131 247 173 305 4.43e-16 77.0

Find 47 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACCTAATAGGGCTTATT+TGG 0.104253 5.2:+18864472 MS.gene64175:CDS
CTGGTCTCATTGGTTCCTTT+TGG 0.214283 5.2:+18864336 MS.gene64175:CDS
CTCTTAGTTGTGTCTCTTGC+TGG 0.223492 5.2:+18864317 MS.gene64175:CDS
ATGTCTGCTGCATTGTTTAT+TGG 0.243435 5.2:-18865066 None:intergenic
TATTATCTTGCTAGTTTGTT+TGG 0.252856 5.2:+18864406 MS.gene64175:CDS
TATTCCAAATAAGCCCTATT+AGG 0.318593 5.2:-18864476 None:intergenic
TTAATTGTGGTTTATTTGAT+TGG 0.318944 5.2:+18865222 MS.gene64175:CDS
CAATCATTGGTGCTGGAATC+TGG 0.324358 5.2:+18864225 MS.gene64175:CDS
ATGGTATGATTGAACCTAAT+AGG 0.329364 5.2:+18864462 MS.gene64175:CDS
TTGCAACAAGGTAGAATATA+AGG 0.332287 5.2:-18864373 None:intergenic
GCGAAAGAGATCCTCAGTTT+TGG 0.353207 5.2:-18865265 None:intergenic
GTGTCTCTTGCTGGTCTCAT+TGG 0.354014 5.2:+18864326 MS.gene64175:CDS
GGCTTATTTGGAATACCGTT+TGG 0.361064 5.2:+18864484 MS.gene64175:CDS
CATTTGTGAACCCAACATAC+TGG 0.397625 5.2:+18865037 MS.gene64175:CDS
CTCATGCAAGGCTGGTTTGT+TGG 0.405503 5.2:+18865140 MS.gene64175:CDS
TTGGACTAATCCAGTATGTT+GGG 0.406980 5.2:-18865047 None:intergenic
CAGATTCCAGCACCAATGAT+TGG 0.408669 5.2:-18864224 None:intergenic
ATCCTTTCAATCCCAATCAT+TGG 0.408710 5.2:+18864212 MS.gene64175:CDS
TGCATTATTTATACCTGCAT+TGG 0.409082 5.2:-18864653 None:intergenic
AGTTGTAACAAAGTTGTGTT+TGG 0.435133 5.2:-18865113 None:intergenic
AGATTCCAGCACCAATGATT+GGG 0.447069 5.2:-18864223 None:intergenic
TGGTATGATTGAACCTAATA+GGG 0.447157 5.2:+18864463 MS.gene64175:CDS
TTTGTTGGCAAATCTTAGAA+AGG 0.458886 5.2:+18865155 MS.gene64175:CDS
GCAATGAGCAACAACATCAT+AGG 0.463723 5.2:+18864167 MS.gene64175:CDS
ATTGGACTAATCCAGTATGT+TGG 0.468680 5.2:-18865048 None:intergenic
GTGTACATGGTTACTCTTCG+TGG 0.469403 5.2:+18864437 MS.gene64175:CDS
CATGTGTCAAGTTTCTTCAA+TGG 0.476338 5.2:+18864270 MS.gene64175:CDS
CACCAATGATTGGGATTGAA+AGG 0.479147 5.2:-18864214 None:intergenic
TCAATCCCAATCATTGGTGC+TGG 0.486549 5.2:+18864218 MS.gene64175:CDS
AACTGCGACTCATGCAAGGC+TGG 0.493492 5.2:+18865132 MS.gene64175:CDS
CAGACATGGATTGTCTTCAA+TGG 0.495893 5.2:+18865082 MS.gene64175:CDS
CAATGGCCAGTCATAATCCT+AGG 0.501117 5.2:+18864287 MS.gene64175:CDS
TTGAAAGGATTACAGCTACT+AGG 0.501297 5.2:-18864199 None:intergenic
ATGGTTACTCTTCGTGGTCA+TGG 0.501755 5.2:+18864443 MS.gene64175:CDS
GAGACATGAGATTCTCCAAA+AGG 0.516594 5.2:-18864351 None:intergenic
TGGCAAATCTTAGAAAGGAA+TGG 0.530252 5.2:+18865160 MS.gene64175:CDS
GTGTATGCACATTGTGTTGG+TGG 0.546354 5.2:-18865015 None:intergenic
TGCACGTCTTACACCAATGC+AGG 0.553446 5.2:+18864640 MS.gene64175:CDS
AATAAACAATGCAGCAGACA+TGG 0.558636 5.2:+18865068 MS.gene64175:CDS
CTCTTTCGCAAGTACAAACA+AGG 0.567497 5.2:+18865279 MS.gene64175:CDS
AACTTGACACATGTGTCTGC+TGG 0.569579 5.2:-18864260 None:intergenic
TTACAACTGCGACTCATGCA+AGG 0.584340 5.2:+18865128 MS.gene64175:CDS
AGAACACCTAGGATTATGAC+TGG 0.617019 5.2:-18864293 None:intergenic
AATGTGTATGCACATTGTGT+TGG 0.656084 5.2:-18865018 None:intergenic
TAAGCACAAGCATTGCAACA+AGG 0.674562 5.2:-18864385 None:intergenic
CAACTAAGAGAAGAACACCT+AGG 0.686330 5.2:-18864304 None:intergenic
CTTAGTGAAACAAATATGTG+TGG 0.704201 5.2:+18864575 MS.gene64175:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGACTGAAAAATAATTAAAG+GGG - chr5.2:18864983-18865002 None:intergenic 20.0%
!! CAGACTGAAAAATAATTAAA+GGG - chr5.2:18864984-18865003 None:intergenic 20.0%
!! TTAAAGGGGAAAAAAATTAT+TGG - chr5.2:18864969-18864988 None:intergenic 20.0%
!! TTAATTGTGGTTTATTTGAT+TGG + chr5.2:18865222-18865241 MS.gene64175:CDS 20.0%
!!! CTTTAATTATTTTTCAGTCT+GGG + chr5.2:18864983-18865002 MS.gene64175:intron 20.0%
! CCAGACTGAAAAATAATTAA+AGG - chr5.2:18864985-18865004 None:intergenic 25.0%
!!! AGTTAGAAGTTCTTTTAAGT+GGG + chr5.2:18864535-18864554 MS.gene64175:CDS 25.0%
!!! CCTTTAATTATTTTTCAGTC+TGG + chr5.2:18864982-18865001 MS.gene64175:intron 25.0%
!!! TATTATCTTGCTAGTTTGTT+TGG + chr5.2:18864406-18864425 MS.gene64175:CDS 25.0%
!!! TTTGGTTATTTTTGTGTACA+TGG + chr5.2:18864424-18864443 MS.gene64175:CDS 25.0%
AGTTGTAACAAAGTTGTGTT+TGG - chr5.2:18865116-18865135 None:intergenic 30.0%
ATCTCAAGATAAACCCATTT+CGG + chr5.2:18864762-18864781 MS.gene64175:intron 30.0%
ATGGTATGATTGAACCTAAT+AGG + chr5.2:18864462-18864481 MS.gene64175:CDS 30.0%
CTTAGTGAAACAAATATGTG+TGG + chr5.2:18864575-18864594 MS.gene64175:CDS 30.0%
TATTCCAAATAAGCCCTATT+AGG - chr5.2:18864479-18864498 None:intergenic 30.0%
TGCATTATTTATACCTGCAT+TGG - chr5.2:18864656-18864675 None:intergenic 30.0%
TGGTATGATTGAACCTAATA+GGG + chr5.2:18864463-18864482 MS.gene64175:CDS 30.0%
TTGCAACAAGGTAGAATATA+AGG - chr5.2:18864376-18864395 None:intergenic 30.0%
TTTGTTGGCAAATCTTAGAA+AGG + chr5.2:18865155-18865174 MS.gene64175:CDS 30.0%
! GAGTTAGAAGTTCTTTTAAG+TGG + chr5.2:18864534-18864553 MS.gene64175:CDS 30.0%
! GTTGAATCAAAGTTTTCGTA+TGG + chr5.2:18864601-18864620 MS.gene64175:CDS 30.0%
!!! CAGTGTTGTTGTTTTAATTG+TGG + chr5.2:18865209-18865228 MS.gene64175:CDS 30.0%
AATAAACAATGCAGCAGACA+TGG + chr5.2:18865068-18865087 MS.gene64175:CDS 35.0%
AATGTGTATGCACATTGTGT+TGG - chr5.2:18865021-18865040 None:intergenic 35.0%
ACCCAGAAAAATCATCCAAA+CGG - chr5.2:18864502-18864521 None:intergenic 35.0%
ATCCTTTCAATCCCAATCAT+TGG + chr5.2:18864212-18864231 MS.gene64175:CDS 35.0%
ATGTCTGCTGCATTGTTTAT+TGG - chr5.2:18865069-18865088 None:intergenic 35.0%
ATTGGACTAATCCAGTATGT+TGG - chr5.2:18865051-18865070 None:intergenic 35.0%
CATGTGTCAAGTTTCTTCAA+TGG + chr5.2:18864270-18864289 MS.gene64175:CDS 35.0%
TGAACCTAATAGGGCTTATT+TGG + chr5.2:18864472-18864491 MS.gene64175:CDS 35.0%
TGGCAAATCTTAGAAAGGAA+TGG + chr5.2:18865160-18865179 MS.gene64175:CDS 35.0%
TTGAAAGGATTACAGCTACT+AGG - chr5.2:18864202-18864221 None:intergenic 35.0%
TTGGACTAATCCAGTATGTT+GGG - chr5.2:18865050-18865069 None:intergenic 35.0%
! ACCGTTTGGATGATTTTTCT+GGG + chr5.2:18864498-18864517 MS.gene64175:CDS 35.0%
! GATCTGTATCTAGTTTCACT+AGG - chr5.2:18864724-18864743 None:intergenic 35.0%
! TACCGTTTGGATGATTTTTC+TGG + chr5.2:18864497-18864516 MS.gene64175:CDS 35.0%
!! GTGACATTGACTTGAAATCT+GGG + chr5.2:18864793-18864812 MS.gene64175:intron 35.0%
!! TGATTGGTTGCTTTGCTTTT+AGG + chr5.2:18865238-18865257 MS.gene64175:CDS 35.0%
!! TTTTAGGAATGCCAAAACTG+AGG + chr5.2:18865254-18865273 MS.gene64175:CDS 35.0%
AGAACACCTAGGATTATGAC+TGG - chr5.2:18864296-18864315 None:intergenic 40.0%
AGATTCCAGCACCAATGATT+GGG - chr5.2:18864226-18864245 None:intergenic 40.0%
CAACTAAGAGAAGAACACCT+AGG - chr5.2:18864307-18864326 None:intergenic 40.0%
CAAGATAAACCCATTTCGGT+TGG + chr5.2:18864766-18864785 MS.gene64175:intron 40.0%
CACCAATGATTGGGATTGAA+AGG - chr5.2:18864217-18864236 None:intergenic 40.0%
CATTTGTGAACCCAACATAC+TGG + chr5.2:18865037-18865056 MS.gene64175:CDS 40.0%
CTCTTTCGCAAGTACAAACA+AGG + chr5.2:18865279-18865298 MS.gene64175:CDS 40.0%
GAGACATGAGATTCTCCAAA+AGG - chr5.2:18864354-18864373 None:intergenic 40.0%
GCAATGAGCAACAACATCAT+AGG + chr5.2:18864167-18864186 MS.gene64175:CDS 40.0%
! CAGACATGGATTGTCTTCAA+TGG + chr5.2:18865082-18865101 MS.gene64175:CDS 40.0%
! GGCTTATTTGGAATACCGTT+TGG + chr5.2:18864484-18864503 MS.gene64175:CDS 40.0%
! TAAGCACAAGCATTGCAACA+AGG - chr5.2:18864388-18864407 None:intergenic 40.0%
! TCAAAGTTTTCGTATGGCTC+AGG + chr5.2:18864607-18864626 MS.gene64175:CDS 40.0%
!! GGTGACATTGACTTGAAATC+TGG + chr5.2:18864792-18864811 MS.gene64175:intron 40.0%
AACTTGACACATGTGTCTGC+TGG - chr5.2:18864263-18864282 None:intergenic 45.0%
ATGGTTACTCTTCGTGGTCA+TGG + chr5.2:18864443-18864462 MS.gene64175:CDS 45.0%
CAATGGCCAGTCATAATCCT+AGG + chr5.2:18864287-18864306 MS.gene64175:CDS 45.0%
CAGATTCCAGCACCAATGAT+TGG - chr5.2:18864227-18864246 None:intergenic 45.0%
CTCTTAGTTGTGTCTCTTGC+TGG + chr5.2:18864317-18864336 MS.gene64175:CDS 45.0%
GTGTACATGGTTACTCTTCG+TGG + chr5.2:18864437-18864456 MS.gene64175:CDS 45.0%
GTGTATGCACATTGTGTTGG+TGG - chr5.2:18865018-18865037 None:intergenic 45.0%
TCAATCCCAATCATTGGTGC+TGG + chr5.2:18864218-18864237 MS.gene64175:CDS 45.0%
TTACAACTGCGACTCATGCA+AGG + chr5.2:18865128-18865147 MS.gene64175:CDS 45.0%
! GCGAAAGAGATCCTCAGTTT+TGG - chr5.2:18865268-18865287 None:intergenic 45.0%
! TAAACCCATTTCGGTTGGTC+TGG + chr5.2:18864771-18864790 MS.gene64175:intron 45.0%
!! CAATCATTGGTGCTGGAATC+TGG + chr5.2:18864225-18864244 MS.gene64175:CDS 45.0%
!!! CTGGTCTCATTGGTTCCTTT+TGG + chr5.2:18864336-18864355 MS.gene64175:CDS 45.0%
GTCACCAGACCAACCGAAAT+GGG - chr5.2:18864778-18864797 None:intergenic 50.0%
GTGTGTTTCTCGTCTCAGGT+TGG + chr5.2:18864817-18864836 MS.gene64175:intron 50.0%
TGCACGTCTTACACCAATGC+AGG + chr5.2:18864640-18864659 MS.gene64175:CDS 50.0%
TGTCACCAGACCAACCGAAA+TGG - chr5.2:18864779-18864798 None:intergenic 50.0%
! GTGTCTCTTGCTGGTCTCAT+TGG + chr5.2:18864326-18864345 MS.gene64175:CDS 50.0%
!! CTCATGCAAGGCTGGTTTGT+TGG + chr5.2:18865140-18865159 MS.gene64175:CDS 50.0%
AACTGCGACTCATGCAAGGC+TGG + chr5.2:18865132-18865151 MS.gene64175:CDS 55.0%
! GGGAGTGTGTTTCTCGTCTC+AGG + chr5.2:18864813-18864832 MS.gene64175:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 18864164 18865311 18864164 ID=MS.gene64175
chr5.2 mRNA 18864164 18865311 18864164 ID=MS.gene64175.t1;Parent=MS.gene64175
chr5.2 exon 18864164 18864661 18864164 ID=MS.gene64175.t1.exon1;Parent=MS.gene64175.t1
chr5.2 CDS 18864164 18864661 18864164 ID=cds.MS.gene64175.t1;Parent=MS.gene64175.t1
chr5.2 exon 18865000 18865311 18865000 ID=MS.gene64175.t1.exon2;Parent=MS.gene64175.t1
chr5.2 CDS 18865000 18865311 18865000 ID=cds.MS.gene64175.t1;Parent=MS.gene64175.t1
Gene Sequence

>MS.gene64175

ATGGCAATGAGCAACAACATCATAGGATTCATAAACCTAGTAGCTGTAATCCTTTCAATCCCAATCATTGGTGCTGGAATCTGGCTAACAAACGAACCAGCAGACACATGTGTCAAGTTTCTTCAATGGCCAGTCATAATCCTAGGTGTTCTTCTCTTAGTTGTGTCTCTTGCTGGTCTCATTGGTTCCTTTTGGAGAATCTCATGTCTCCTTATATTCTACCTTGTTGCAATGCTTGTGCTTATTATCTTGCTAGTTTGTTTGGTTATTTTTGTGTACATGGTTACTCTTCGTGGTCATGGTATGATTGAACCTAATAGGGCTTATTTGGAATACCGTTTGGATGATTTTTCTGGGTTTTTGAAAAGAAGAGTTAGAAGTTCTTTTAAGTGGGATGCTATTAGAAGTTGTCTTAGTGAAACAAATATGTGTGGTGAGTTGAATCAAAGTTTTCGTATGGCTCAGGACTTCTTTAATGCACGTCTTACACCAATGCAGGTATAAATAATGCAATTTTTTTAGTTTATTTTACACATTTTCTCTCTACTGTACTTGATCCTAGTGAAACTAGATACAGATCTTTAAGTAAGAAAGTAAGATCTCAAGATAAACCCATTTCGGTTGGTCTGGTGACATTGACTTGAAATCTGGGAGTGTGTTTCTCGTCTCAGGTTGGATAAATGATTGAAAGAAAATTAGTTATTACTAGTATTAACAATAAAAAATAAACAAATTACTGTTTGATAATTAAAAAAAATTCACAAAAATAGTTTCTGAGTTTTCTTTACTTACATATATTTTGCCAATAATTTTTTTCCCCTTTAATTATTTTTCAGTCTGGGTGCTGCAAGCCACCAACACAATGTGCATACACATTTGTGAACCCAACATACTGGATTAGTCCAATAAACAATGCAGCAGACATGGATTGTCTTCAATGGAGCAATGACCAAACACAACTTTGTTACAACTGCGACTCATGCAAGGCTGGTTTGTTGGCAAATCTTAGAAAGGAATGGAAAAGAGCTAATGTGATATTGATCATCAGTGTTGTTGTTTTAATTGTGGTTTATTTGATTGGTTGCTTTGCTTTTAGGAATGCCAAAACTGAGGATCTCTTTCGCAAGTACAAACAAGGCTACACTTGA

Protein sequence

>MS.gene64175.t1

MAMSNNIIGFINLVAVILSIPIIGAGIWLTNEPADTCVKFLQWPVIILGVLLLVVSLAGLIGSFWRISCLLIFYLVAMLVLIILLVCLVIFVYMVTLRGHGMIEPNRAYLEYRLDDFSGFLKRRVRSSFKWDAIRSCLSETNMCGELNQSFRMAQDFFNARLTPMQSGCCKPPTQCAYTFVNPTYWISPINNAADMDCLQWSNDQTQLCYNCDSCKAGLLANLRKEWKRANVILIISVVVLIVVYLIGCFAFRNAKTEDLFRKYKQGYT