Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64418.t1 | XP_013463516.1 | 100 | 114 | 0 | 0 | 1 | 114 | 1 | 114 | 2.90E-40 | 174.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64418.t1 | Q570U6 | 57.1 | 112 | 48 | 0 | 1 | 112 | 1 | 112 | 3.8e-25 | 115.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64418.t1 | I3TAT7 | 100.0 | 114 | 0 | 0 | 1 | 114 | 1 | 114 | 2.1e-40 | 174.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050787 | MS.gene64418 | 0.800577 | 1.35E-48 | -1.69E-46 |
| MS.gene051207 | MS.gene64418 | 0.805691 | 1.18E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene64418 | 0.812589 | 3.93E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene64418 | 0.805471 | 1.31E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64418.t1 | MTR_2g041310 | 100.000 | 114 | 0 | 0 | 1 | 114 | 1 | 114 | 4.36e-80 | 230 |
| MS.gene64418.t1 | MTR_4g125590 | 82.456 | 114 | 19 | 1 | 1 | 113 | 1 | 114 | 4.77e-64 | 190 |
| MS.gene64418.t1 | MTR_8g020200 | 70.175 | 114 | 34 | 0 | 1 | 114 | 1 | 114 | 6.06e-54 | 164 |
| MS.gene64418.t1 | MTR_3g070490 | 47.500 | 80 | 42 | 0 | 6 | 85 | 36 | 115 | 1.23e-22 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64418.t1 | AT1G17455 | 70.536 | 112 | 33 | 0 | 1 | 112 | 1 | 112 | 2.00e-51 | 158 |
| MS.gene64418.t1 | AT1G17455 | 70.536 | 112 | 33 | 0 | 1 | 112 | 1 | 112 | 2.00e-51 | 158 |
| MS.gene64418.t1 | AT2G06255 | 68.696 | 115 | 29 | 3 | 1 | 114 | 1 | 109 | 2.36e-48 | 150 |
| MS.gene64418.t1 | AT1G72630 | 66.667 | 114 | 37 | 1 | 1 | 113 | 5 | 118 | 3.99e-48 | 150 |
| MS.gene64418.t1 | AT2G29950 | 51.351 | 74 | 36 | 0 | 16 | 89 | 34 | 107 | 1.60e-20 | 80.5 |
| MS.gene64418.t1 | AT2G40080 | 48.571 | 70 | 36 | 0 | 16 | 85 | 22 | 91 | 2.32e-20 | 79.7 |
Find 17 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGAGATTATCAGGAATTT+TGG | 0.137257 | 2.1:-49383145 | None:intergenic |
| TGTACAAGTGCAGAACATTT+TGG | 0.228918 | 2.1:+49383077 | MS.gene64418:CDS |
| ACATTTCTGCTGAGATTATC+AGG | 0.272078 | 2.1:-49383153 | None:intergenic |
| CAAATTGATACTAAGATATT+AGG | 0.308401 | 2.1:+49383036 | MS.gene64418:CDS |
| GATGATACATTCTCTGGTAT+TGG | 0.392337 | 2.1:+49383003 | MS.gene64418:CDS |
| ACATTCTCTGGTATTGGAAA+TGG | 0.427951 | 2.1:+49383009 | MS.gene64418:CDS |
| ATGGAGGATGATACATTCTC+TGG | 0.503053 | 2.1:+49382997 | MS.gene64418:CDS |
| TCCATGGATGTTACTTCAGA+AGG | 0.520708 | 2.1:+49383255 | MS.gene64418:CDS |
| TCCTTCTGAAGTAACATCCA+TGG | 0.529648 | 2.1:-49383256 | None:intergenic |
| GATAATCTCAGCAGAAATGT+TGG | 0.548630 | 2.1:+49383156 | MS.gene64418:CDS |
| AACAAACCCTCTTTCTATGT+AGG | 0.557195 | 2.1:-49383333 | None:intergenic |
| AGTGGTGCTGTGAAATCAGA+TGG | 0.588608 | 2.1:+49383285 | MS.gene64418:CDS |
| CAACAACAATATTAGAAGAG+TGG | 0.606391 | 2.1:+49383194 | MS.gene64418:CDS |
| GTGAAATCAGATGGAAAAGG+TGG | 0.635941 | 2.1:+49383294 | MS.gene64418:CDS |
| ACTTCAGAAGGAGATTCAAG+TGG | 0.659737 | 2.1:+49383267 | MS.gene64418:CDS |
| GCTGTGAAATCAGATGGAAA+AGG | 0.691564 | 2.1:+49383291 | MS.gene64418:CDS |
| AAGGTGGACACAAAAGACAC+AGG | 0.696481 | 2.1:+49383310 | MS.gene64418:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAATTGATACTAAGATATT+AGG | + | chr2.1:49383036-49383055 | MS.gene64418:CDS | 20.0% |
| !! | AAGTTCTTTTACTAAATCCA+TGG | + | chr2.1:49383239-49383258 | MS.gene64418:CDS | 25.0% |
| !! | GAATTTTGGATTCATGATTT+TGG | - | chr2.1:49383134-49383153 | None:intergenic | 25.0% |
| CAACAACAATATTAGAAGAG+TGG | + | chr2.1:49383194-49383213 | MS.gene64418:CDS | 30.0% | |
| ACATTTCTGCTGAGATTATC+AGG | - | chr2.1:49383156-49383175 | None:intergenic | 35.0% | |
| GATAATCTCAGCAGAAATGT+TGG | + | chr2.1:49383156-49383175 | MS.gene64418:CDS | 35.0% | |
| GATGATACATTCTCTGGTAT+TGG | + | chr2.1:49383003-49383022 | MS.gene64418:CDS | 35.0% | |
| ! | ACATTCTCTGGTATTGGAAA+TGG | + | chr2.1:49383009-49383028 | MS.gene64418:CDS | 35.0% |
| ! | AGAACATTTTGGATCAGAAC+AGG | + | chr2.1:49383088-49383107 | MS.gene64418:CDS | 35.0% |
| ! | GCTGAGATTATCAGGAATTT+TGG | - | chr2.1:49383148-49383167 | None:intergenic | 35.0% |
| ! | TGTACAAGTGCAGAACATTT+TGG | + | chr2.1:49383077-49383096 | MS.gene64418:CDS | 35.0% |
| ACTTCAGAAGGAGATTCAAG+TGG | + | chr2.1:49383267-49383286 | MS.gene64418:CDS | 40.0% | |
| ATGGAGGATGATACATTCTC+TGG | + | chr2.1:49382997-49383016 | MS.gene64418:CDS | 40.0% | |
| GCTGTGAAATCAGATGGAAA+AGG | + | chr2.1:49383291-49383310 | MS.gene64418:CDS | 40.0% | |
| GTGAAATCAGATGGAAAAGG+TGG | + | chr2.1:49383294-49383313 | MS.gene64418:CDS | 40.0% | |
| ! | TCCATGGATGTTACTTCAGA+AGG | + | chr2.1:49383255-49383274 | MS.gene64418:CDS | 40.0% |
| !! | TCCTTCTGAAGTAACATCCA+TGG | - | chr2.1:49383259-49383278 | None:intergenic | 40.0% |
| AAGGTGGACACAAAAGACAC+AGG | + | chr2.1:49383310-49383329 | MS.gene64418:CDS | 45.0% | |
| !! | AGTGGTGCTGTGAAATCAGA+TGG | + | chr2.1:49383285-49383304 | MS.gene64418:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 49382997 | 49383341 | 49382997 | ID=MS.gene64418 |
| chr2.1 | mRNA | 49382997 | 49383341 | 49382997 | ID=MS.gene64418.t1;Parent=MS.gene64418 |
| chr2.1 | exon | 49382997 | 49383341 | 49382997 | ID=MS.gene64418.t1.exon1;Parent=MS.gene64418.t1 |
| chr2.1 | CDS | 49382997 | 49383341 | 49382997 | ID=cds.MS.gene64418.t1;Parent=MS.gene64418.t1 |
| Gene Sequence |
| Protein sequence |
